MD12G1126200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015460
Physical Location & Seq
Forward (+)
20155007 .. 20156579
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1126200.v1.1.491

Sequence Viewer

Length: 537 bp
ATGTTAAGCCATGAGATGATCAGCCTGCAGCAGGTTTGCATTGGATTGGAATATTCTTTTGGTTTCTGGGATGTGCTGCTGCAAATGGATATTCAAGCTCGAATAGGAAGCTTATCATCCTCCTCTCACAACTTTCGGACCTCTCGAAAACATAACTCTTCATCTTCTTGTCCATCTTCTTGCTACTCGCCATTGAAGCCCTTGTGTTCCAGAAGGGATTCTCAGGAGCCCCAACAAAACGGTGACAACAAGGGTGATAAATTCTCAACAGATTGGGACAAGGCATGGGCAAACATAAGGAAACAAGGCAAAAACAAAAAGAAGGGTGGCTTCTTCTCAGGGTTTTCTTCTCCAAACAAGTACGTGAGCTGGAACCCTAGGCAGTCCAATTACCCACTGTCTGAGGAGGTTGATCCAATCAAGAGGACAGAAAGGTCCAACCTCATGCTATGGACTAGTCCAAGGTTTACCCTAGCAGGAGCCATTGTTATTGTCACCTTCCTTTTGGTATACACCATTCTTTATCCACTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.3

Weight (kDa)

9.54

Isoelectric Point (pI)

63.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53470 AT3G53470
fragaria_vesca FvH4_6g15150 FvH4_6g15150
malus_domestica MD12G1126200.v1.1
prunus_persica Prupe.6G242800_v2.0.a1
pyrus_communis pycom12g12440
rosa_chinensis RchiOBHm_Chr3g0468451
rosa_laevigata RLG00000024428
rosa_multiflora Rmu_sc0040156.1_g000005
rosa_roxburghii Rroxscaffold_6G00412750
rosa_rugosa Rorug03G0095500
rosa_samantha Rh3AG143800 Rh3BG165800 Rh3DG165400
rosa_wichuraiana Rw3G013570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 433
Acc36I ACCTGC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 510
AclWI GGATC 1 cut(s) 407
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 1 cut(s) 31
AflII CTTAAG 1 cut(s) 530
AgsI TTSAA 2 cut(s) 95, 196
AhlI ACTAGT 1 cut(s) 455
AjuI GAANNNNNNNTTGG 2 cut(s) 42, 74
AluBI AGCT 3 cut(s) 98, 111, 369
AluI AGCT 3 cut(s) 98, 111, 369
AlwI GGATC 1 cut(s) 407
ApeKI GCWGC 3 cut(s) 28, 76, 79
ApoI RAATTY 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 217
AspA2I CCTAGG 1 cut(s) 377
AspS9I GGNCC 2 cut(s) 138, 435
AsuHPI GGTGA 3 cut(s) 254, 266, 487
AvaII GGWCC 2 cut(s) 138, 435
AvrII CCTAGG 1 cut(s) 377
BanII GRGCYC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 40, 63, 66
BccI CCATC 1 cut(s) 181
BclI TGATCA 1 cut(s) 18
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 3 cut(s) 378, 456, 473
BfmI CTRYAG 1 cut(s) 26
BfrI CTTAAG 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 3 cut(s) 29, 77, 80
BlnI CCTAGG 1 cut(s) 377
BlsI GCNGC 3 cut(s) 30, 78, 81
Bme18I GGWCC 2 cut(s) 138, 435
BmgT120I GGNCC 2 cut(s) 138, 435
BmiI GGNNCC 3 cut(s) 228, 374, 481
BsaAI YACGTR 1 cut(s) 364
BsaJI CCNNGG 2 cut(s) 377, 461
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 1 cut(s) 31
BseDI CCNNGG 2 cut(s) 377, 461
BseGI GGATG 2 cut(s) 76, 116
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMII CTCAG 3 cut(s) 236, 351, 393
BseRI GAGGAG 2 cut(s) 112, 419
BseXI GCAGC 3 cut(s) 40, 63, 66
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 2 cut(s) 18, 412
BspCNI CTCAG 3 cut(s) 235, 350, 394
BspLI GGNNCC 3 cut(s) 228, 374, 481
BspMAI CTGCAG 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 1 cut(s) 407
BspTI CTTAAG 1 cut(s) 530
BssECI CCNNGG 2 cut(s) 377, 461
BssMI GATC 2 cut(s) 18, 412
BssNAI GTATAC 1 cut(s) 511
BssT1I CCWWGG 2 cut(s) 377, 461
Bst1107I GTATAC 1 cut(s) 511
Bst4CI ACNGT 2 cut(s) 242, 399
Bst6I CTCTTC 1 cut(s) 163
BstAFI CTTAAG 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 3 cut(s) 222, 337, 402
BstENI CCTNNNNNAGG 1 cut(s) 29
BstF5I GGATG 2 cut(s) 76, 116
BstKTI GATC 2 cut(s) 21, 415
BstMBI GATC 2 cut(s) 18, 412
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstSFI CTRYAG 1 cut(s) 26
BstV1I GCAGC 3 cut(s) 40, 63, 66
BstZ17I GTATAC 1 cut(s) 511
BtsCI GGATG 2 cut(s) 76, 116
BtsIMutI CAGTG 1 cut(s) 395
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 138, 435
Csp6I GTAC 1 cut(s) 361
CviAII CATG 3 cut(s) 11, 285, 445
CviJI RGCY 9 cut(s) 9, 24, 98, 111, 199, 229, 330, 369, 482
CviKI_1 RGCY 9 cut(s) 9, 24, 98, 111, 199, 229, 330, 369, 482
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 3 cut(s) 222, 337, 402
DpnI GATC 2 cut(s) 20, 414
DpnII GATC 2 cut(s) 18, 412
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 377, 461
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 138, 435
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoT14I CCWWGG 2 cut(s) 377, 461
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 2 cut(s) 377, 461
FaeI CATG 3 cut(s) 14, 288, 448
FaiI YATR 7 cut(s) 12, 153, 286, 296, 446, 451, 511
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 290
FatI CATG 3 cut(s) 10, 284, 444
FbaI TGATCA 1 cut(s) 18
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 3 cut(s) 29, 77, 80
FokI GGATG 2 cut(s) 83, 103
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 3 cut(s) 29, 77, 80
FspBI CTAG 3 cut(s) 378, 456, 473
GluI GCNGC 3 cut(s) 29, 77, 80
Hin1II CATG 3 cut(s) 14, 288, 448
HindIII AAGCTT 1 cut(s) 109
HinfI GANTC 1 cut(s) 218
HphI GGTGA 3 cut(s) 254, 266, 487
Hpy166II GTNNAC 2 cut(s) 468, 511
Hpy188I TCNGA 2 cut(s) 138, 403
Hpy188III TCNNGA 4 cut(s) 144, 210, 224, 421
Hpy8I GTNNAC 2 cut(s) 468, 511
HpyAV CCTTC 3 cut(s) 207, 316, 508
HpyCH4III ACNGT 2 cut(s) 242, 399
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 3 cut(s) 28, 39, 82
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 3 cut(s) 222, 337, 402
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 3 cut(s) 14, 288, 448
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 2 cut(s) 18, 412
LmnI GCTCC 2 cut(s) 226, 479
LpnPI CCDG 8 cut(s) 17, 38, 52, 209, 223, 324, 355, 462
Lsp1109I GCAGC 3 cut(s) 40, 63, 66
MaeI CTAG 3 cut(s) 378, 456, 473
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 242, 493
MalI GATC 2 cut(s) 20, 414
MboI GATC 2 cut(s) 18, 412
MboII GAAGA 5 cut(s) 150, 156, 168, 325, 339
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 2 cut(s) 260, 388
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 7 cut(s) 130, 133, 151, 397, 400, 417, 452
MroXI GAANNNNTTC 1 cut(s) 217
MseI TTAA 2 cut(s) 5, 531
MspCI CTTAAG 1 cut(s) 530
MwoI GCNNNNNNNGC 1 cut(s) 196
NdeII GATC 2 cut(s) 18, 412
NlaIII CATG 3 cut(s) 14, 288, 448
NlaIV GGNNCC 3 cut(s) 228, 374, 481
NmuCI GTSAC 2 cut(s) 242, 493
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 3 cut(s) 30, 78, 81
Ppu21I YACGTR 1 cut(s) 364
PspN4I GGNNCC 3 cut(s) 228, 374, 481
PspPI GGNCC 2 cut(s) 138, 435
PstI CTGCAG 1 cut(s) 30
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SaqAI TTAA 2 cut(s) 5, 531
SatI GCNGC 3 cut(s) 29, 77, 80
Sau3AI GATC 2 cut(s) 18, 412
Sau96I GGNCC 2 cut(s) 138, 435
SduI GDGCHC 1 cut(s) 231
SfcI CTRYAG 1 cut(s) 26
SinI GGWCC 2 cut(s) 138, 435
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SpeI ACTAGT 1 cut(s) 455
Sse9I AATT 2 cut(s) 260, 388
SspI AATATT 1 cut(s) 53
SspMI CTAG 3 cut(s) 378, 456, 473
StyI CCWWGG 2 cut(s) 377, 461
TaaI ACNGT 2 cut(s) 242, 399
TaiI ACGT 1 cut(s) 366
TaqI TCGA 2 cut(s) 100, 145
TasI AATT 2 cut(s) 260, 388
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 2 cut(s) 5, 531
Tru9I TTAA 2 cut(s) 5, 531
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 2 cut(s) 242, 493
TseI GCWGC 3 cut(s) 28, 76, 79
Tsp45I GTSAC 2 cut(s) 242, 493
TspDTI ATGAA 1 cut(s) 150
TspRI CASTG 1 cut(s) 402
Vha464I CTTAAG 1 cut(s) 530
VpaK11BI GGWCC 2 cut(s) 138, 435
XagI CCTNNNNNAGG 1 cut(s) 29
XapI RAATTY 1 cut(s) 260
XmaJI CCTAGG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 510
XmnI GAANNNNTTC 1 cut(s) 217
XspI CTAG 3 cut(s) 378, 456, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.