Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005963

Physical Location & Seq

Genomic Coordinates Reverse (-)

20677551 .. 20680759

Gene Structure 3209 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1131700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 930 bp

ATGCCTCGTTATGATGATCGATATGGCAACACCCGTCTTTATGTTGGTCGGTTGTCTTCACGCACGCGTTCACGTGATCTAGAACGGCTGTTCAACAGATATGGAAGAGTACGTGATGTGGACATGAAGCGGGAGTTTGCTTTTGTTGACTTTAGTGATCCTCGAGATGCTGATGATGCAAGATATAGCTTAGATGGTCGGGAATTTGATGGAAGTCGTCTCATTGTGGAATTTGCAAAAGGGGCACCACGTGGTTCGCGGGAATATTTAGGCAGAGGTCCTACTCCTGGATCTGGACGTTGCTTTAATTGTGGCATTGACGGCCACTGGGCTCGTGATTGCAAGGCTGGGGACTGGAAGAATAAGTGCTATCGCTGTGGAGAAAGAGGCCATATTGAAAGGCAGTGCAAAAATAGTCCAAAGGAATTAAGTCGCGGGCGGAGTCGCTCACCAGTTGGATCACGTTCTCGCCGTGGAAGAAGCCGTAGCCCAAGTTACAGCCGAAGCCGCAGCTATAGCCGTTCAAGGTCCCCAGCAAGGAGAGGGCGAAGTGAGGATCGCTATGACAGATCACGGAGCCCTTCTTACAGGAAGAGTCCACTGCCCTCAAAAGGAGGGAGGAAGCGCAGCCTGACACCTGATGTAGACAGTCCTCGTGGGAGACGTAGCCCTTCCCCCAGAAATGGTAGAATGGAAATGGAGAAAGAGGGCTCTGATTACAGCCCCAGGGTAAGAAGTAGAAGTCCGGTTAGCCCCAGGTTAAGGAGCAGAAGTCCTGTTAGCCCTGGTAGAGACAGCCCTGTTGTCAGGAAGTATCCAAGCCCTTCTGAAGCCGATGGTCGCGGTCGCAGTCCCAGTCCCGATCGGAGCCCCGTCGCTGATTATGATGATGACAACCGTCGCACTCCCAGAGGCAGCGAGTCACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0019904 GO:0022607 GO:0022613 GO:0022618 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031503 GO:0031974 GO:0031981 GO:0033036 GO:0033119 GO:0034613 GO:0034622 GO:0034641 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0045934 GO:0046483 GO:0046907 GO:0048024 GO:0048025 GO:0048519 GO:0048523 GO:0050657 GO:0050658 GO:0050684 GO:0050686 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051253 GO:0051641 GO:0051649 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:1901360 GO:1903311 GO:1903312

KEGG Pathways

Metabolic & Signaling

ko03040 ko05168 map03040 map05168

Pfam Domains

Protein Families

RRM_1 zf-CCHC

Protein Analysis

310

Amino Acids

35.33

Weight (kDa)

10.92

Isoelectric Point (pI)

96.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	13 - 74	6.6e-14	RNA recognition motif
zf-CCHC	PF00098	100 - 115	3e-06	Zinc knuckle

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37340 AT2G37340 AT2G37340 AT2G37340 AT2G37340 AT2G37340 AT3G53500 AT3G53500 AT3G53500
fragaria_vesca	FvH4_6g14771 FvH4_6g14771 FvH4_6g14771 FvH4_6g14771
malus_domestica	MD12G1131700.v1.1
prunus_persica	Prupe.6G246100_v2.0.a1 Prupe.6G246100_v2.0.a1 Prupe.6G246100_v2.0.a1
pyrus_communis	pycom12g12750
rosa_chinensis	RchiOBHm_Chr3g0468001
rosa_roxburghii	Rroxscaffold_6G00413220
rosa_rugosa	Rorug03G0091100 Rorug03G0091100 Rorug03G0091200
rosa_samantha	Rh3AG139800 Rh3CG161200 Rh3DG161700
rosa_wichuraiana	Rw3G013220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	244
AccI	GTMKAC	1 cut(s)	645
AccII	CGCG	4 cut(s)	67, 259, 435, 843
AciI	CCGC	6 cut(s)	130, 259, 435, 439, 508, 843
AclWI	GGATC	4 cut(s)	152, 298, 466, 564
AcoI	YGGCCR	1 cut(s)	322
AcsI	RAATTY	2 cut(s)	203, 230
AcuI	CTGAAG	1 cut(s)	849
AcvI	CACGTG	2 cut(s)	74, 251
AdeI	CACNNNGTG	1 cut(s)	251
AfaI	GTAC	1 cut(s)	111
AfiI	CCNNNNNNNGG	6 cut(s)	287, 293, 537, 611, 683, 762
AflIII	ACRYGT	1 cut(s)	65
AgsI	TTSAA	3 cut(s)	94, 398, 525
AjnI	CCWGG	4 cut(s)	286, 725, 755, 784
AluBI	AGCT	2 cut(s)	189, 513
AluI	AGCT	2 cut(s)	189, 513
Alw26I	GTCTC	3 cut(s)	224, 655, 786
AlwI	GGATC	4 cut(s)	152, 298, 466, 564
Ama87I	CYCGRG	1 cut(s)	162
AoxI	GGCC	2 cut(s)	322, 388
ApeKI	GCWGC	3 cut(s)	510, 627, 915
ApoI	RAATTY	2 cut(s)	203, 230
AspLEI	GCGC	1 cut(s)	627
AspS9I	GGNCC	2 cut(s)	278, 528
AsuHPI	GGTGA	2 cut(s)	441, 915
AvaI	CYCGRG	1 cut(s)	162
AvaII	GGWCC	2 cut(s)	278, 528
BaeGI	GKGCMC	1 cut(s)	247
BanI	GGYRCC	1 cut(s)	244
BanII	GRGCYC	4 cut(s)	334, 581, 713, 872
BauI	CACGAG	2 cut(s)	333, 654
BbrPI	CACGTG	2 cut(s)	74, 251
BbsI	GAAGAC	1 cut(s)	48
BbvI	GCAGC	2 cut(s)	522, 639
BccI	CCATC	3 cut(s)	188, 203, 830
BceAI	ACGGC	5 cut(s)	101, 337, 456, 468, 504
BciT130I	CCWGG	4 cut(s)	288, 727, 757, 786
BciVI	GTATCC	1 cut(s)	825
BcoDI	GTCTC	3 cut(s)	224, 655, 786
BfaI	CTAG	1 cut(s)	80
BfmI	CTRYAG	1 cut(s)	514
BfuI	GTATCC	1 cut(s)	825
BisI	GCNGC	4 cut(s)	508, 511, 628, 916
BlsI	GCNGC	4 cut(s)	509, 512, 629, 917
Bme1390I	CCNGG	4 cut(s)	288, 727, 757, 786
Bme18I	GGWCC	2 cut(s)	278, 528
BmeT110I	CYCGRG	1 cut(s)	162
BmgT120I	GGNCC	2 cut(s)	278, 528
BmiI	GGNNCC	4 cut(s)	246, 530, 578, 869
BmrFI	CCNGG	4 cut(s)	288, 727, 757, 786
BmrI	ACTGGG	2 cut(s)	337, 849
BmsI	GCATC	2 cut(s)	157, 166
BmuI	ACTGGG	2 cut(s)	337, 849
BoxI	GACNNNNGTC	1 cut(s)	897
BpiI	GAAGAC	1 cut(s)	48
Bsa29I	ATCGAT	1 cut(s)	19
BsaAI	YACGTR	3 cut(s)	74, 113, 251
BsaJI	CCNNGG	5 cut(s)	472, 725, 726, 755, 784
BsaWI	WCCGGW	1 cut(s)	745
BsaXI	ACNNNNNCTCC	2 cut(s)	757, 787
Bsc4I	CCNNNNNNNGG	6 cut(s)	287, 293, 537, 611, 683, 762
Bse1I	ACTGG	4 cut(s)	332, 359, 452, 855
BseBI	CCWGG	4 cut(s)	288, 727, 757, 786
BseCI	ATCGAT	1 cut(s)	19
BseDI	CCNNGG	5 cut(s)	472, 725, 726, 755, 784
BseLI	CCNNNNNNNGG	6 cut(s)	287, 293, 537, 611, 683, 762
BseNI	ACTGG	4 cut(s)	332, 359, 452, 855
BseSI	GKGCMC	1 cut(s)	247
BseXI	GCAGC	2 cut(s)	522, 639
BseYI	CCCAGC	2 cut(s)	347, 532
Bsh1236I	CGCG	4 cut(s)	67, 259, 435, 843
Bsh1285I	CGRYCG	2 cut(s)	847, 865
BshFI	GGCC	2 cut(s)	324, 390
BshNI	GGYRCC	1 cut(s)	244
BshVI	ATCGAT	1 cut(s)	19
BsiEI	CGRYCG	2 cut(s)	847, 865
BsiHKCI	CYCGRG	1 cut(s)	162
BsiSI	CCGG	1 cut(s)	746
BslFI	GGGAC	4 cut(s)	365, 514, 837, 843
BslI	CCNNNNNNNGG	6 cut(s)	287, 293, 537, 611, 683, 762
BsmAI	GTCTC	3 cut(s)	224, 655, 786
BsmBI	CGTCTC	2 cut(s)	224, 655
BsmFI	GGGAC	4 cut(s)	365, 514, 837, 843
BsnI	GGCC	2 cut(s)	324, 390
BsoBI	CYCGRG	1 cut(s)	162
Bsp1286I	GDGCHC	5 cut(s)	247, 334, 581, 713, 872
Bsp143I	GATC	8 cut(s)	16, 76, 157, 290, 458, 556, 569, 862
BspACI	CCGC	6 cut(s)	130, 259, 435, 439, 508, 843
BspANI	GGCC	2 cut(s)	324, 390
BspDI	ATCGAT	1 cut(s)	19
BspFNI	CGCG	4 cut(s)	67, 259, 435, 843
BspLI	GGNNCC	4 cut(s)	246, 530, 578, 869
BspPI	GGATC	4 cut(s)	152, 298, 466, 564
BspT107I	GGYRCC	1 cut(s)	244
BsrI	ACTGG	4 cut(s)	332, 359, 452, 855
BssECI	CCNNGG	5 cut(s)	472, 725, 726, 755, 784
BssMI	GATC	8 cut(s)	16, 76, 157, 290, 458, 556, 569, 862
BssSI	CACGAG	2 cut(s)	333, 654
Bst2BI	CACGAG	2 cut(s)	333, 654
Bst2UI	CCWGG	4 cut(s)	288, 727, 757, 786
Bst4CI	ACNGT	2 cut(s)	650, 899
Bst6I	CTCTTC	2 cut(s)	100, 587
BstBAI	YACGTR	3 cut(s)	74, 113, 251
BstC8I	GCNNGC	2 cut(s)	65, 437
BstDEI	CTNAG	1 cut(s)	190
BstDSI	CCRYGG	1 cut(s)	472
BstFNI	CGCG	4 cut(s)	67, 259, 435, 843
BstHHI	GCGC	1 cut(s)	627
BstKTI	GATC	8 cut(s)	19, 79, 160, 293, 461, 559, 572, 865
BstMAI	GTCTC	3 cut(s)	224, 655, 786
BstMBI	GATC	8 cut(s)	16, 76, 157, 290, 458, 556, 569, 862
BstMCI	CGRYCG	2 cut(s)	847, 865
BstMWI	GCNNNNNNNGC	5 cut(s)	176, 242, 321, 507, 516
BstNI	CCWGG	4 cut(s)	288, 727, 757, 786
BstPAI	GACNNNNGTC	1 cut(s)	897
BstSCI	CCNGG	4 cut(s)	286, 725, 755, 784
BstSFI	CTRYAG	1 cut(s)	514
BstSLI	GKGCMC	1 cut(s)	247
BstUI	CGCG	4 cut(s)	67, 259, 435, 843
BstV1I	GCAGC	2 cut(s)	522, 639
BstV2I	GAAGAC	1 cut(s)	48
BstX2I	RGATCY	1 cut(s)	290
BstYI	RGATCY	1 cut(s)	290
Bsu15I	ATCGAT	1 cut(s)	19
BsuI	GTATCC	1 cut(s)	825
BsuRI	GGCC	2 cut(s)	324, 390
BsuTUI	ATCGAT	1 cut(s)	19
BtgI	CCRYGG	1 cut(s)	472
BtsI	GCAGTG	2 cut(s)	410, 599
BtsIMutI	CAGTG	3 cut(s)	325, 410, 599
Cac8I	GCNNGC	2 cut(s)	65, 437
CfoI	GCGC	1 cut(s)	627
Cfr13I	GGNCC	2 cut(s)	278, 528
ClaI	ATCGAT	1 cut(s)	19
Csp6I	GTAC	1 cut(s)	110
CviAII	CATG	1 cut(s)	124
CviQI	GTAC	1 cut(s)	110
DdeI	CTNAG	1 cut(s)	190
DpnI	GATC	8 cut(s)	18, 78, 159, 292, 460, 558, 571, 864
DpnII	GATC	8 cut(s)	16, 76, 157, 290, 458, 556, 569, 862
DraIII	CACNNNGTG	1 cut(s)	251
EaeI	YGGCCR	1 cut(s)	322
Eam1104I	CTCTTC	2 cut(s)	100, 587
EarI	CTCTTC	2 cut(s)	100, 587
EciI	GGCGGA	1 cut(s)	454
Eco24I	GRGCYC	4 cut(s)	334, 581, 713, 872
Eco47I	GGWCC	2 cut(s)	278, 528
Eco57I	CTGAAG	1 cut(s)	849
Eco72I	CACGTG	2 cut(s)	74, 251
Eco88I	CYCGRG	1 cut(s)	162
EcoO109I	RGGNCCY	2 cut(s)	278, 528
EcoRII	CCWGG	4 cut(s)	286, 725, 755, 784
EcoT38I	GRGCYC	4 cut(s)	334, 581, 713, 872
Esp3I	CGTCTC	2 cut(s)	224, 655
FaeI	CATG	1 cut(s)	127
FaqI	GGGAC	4 cut(s)	365, 514, 837, 843
FatI	CATG	1 cut(s)	123
FauI	CCCGC	3 cut(s)	123, 252, 428
FblI	GTMKAC	1 cut(s)	645
Fnu4HI	GCNGC	4 cut(s)	508, 511, 628, 916
FriOI	GRGCYC	4 cut(s)	334, 581, 713, 872
Fsp4HI	GCNGC	4 cut(s)	508, 511, 628, 916
FspBI	CTAG	1 cut(s)	80
GlaI	GCGC	1 cut(s)	626
GluI	GCNGC	4 cut(s)	508, 511, 628, 916
GsaI	CCCAGC	2 cut(s)	351, 536
HaeIII	GGCC	2 cut(s)	324, 390
HapII	CCGG	1 cut(s)	746
HhaI	GCGC	1 cut(s)	627
Hin1II	CATG	1 cut(s)	127
Hin6I	GCGC	1 cut(s)	625
HinP1I	GCGC	1 cut(s)	625
HincII	GTYRAC	1 cut(s)	148
HindII	GTYRAC	1 cut(s)	148
HinfI	GANTC	3 cut(s)	442, 595, 920
HpaII	CCGG	1 cut(s)	746
HphI	GGTGA	2 cut(s)	441, 915
Hpy166II	GTNNAC	5 cut(s)	71, 121, 148, 599, 646
Hpy188I	TCNGA	3 cut(s)	715, 829, 867
Hpy188III	TCNNGA	7 cut(s)	80, 164, 200, 294, 335, 808, 860
Hpy8I	GTNNAC	5 cut(s)	71, 121, 148, 599, 646
Hpy99I	CGWCG	2 cut(s)	878, 903
HpyAV	CCTTC	3 cut(s)	591, 681, 834
HpyCH4III	ACNGT	2 cut(s)	650, 899
HpyCH4IV	ACGT	6 cut(s)	73, 112, 250, 298, 463, 664
HpyCH4V	TGCA	4 cut(s)	179, 236, 342, 408
HpyF10VI	GCNNNNNNNGC	5 cut(s)	176, 242, 321, 507, 516
HpyF3I	CTNAG	1 cut(s)	190
HpySE526I	ACGT	6 cut(s)	73, 112, 250, 298, 463, 664
Hsp92II	CATG	1 cut(s)	127
HspAI	GCGC	1 cut(s)	625
Kzo9I	GATC	8 cut(s)	16, 76, 157, 290, 458, 556, 569, 862
LmnI	GCTCC	3 cut(s)	576, 765, 867
Lsp1109I	GCAGC	2 cut(s)	522, 639
LweI	GCATC	2 cut(s)	157, 166
MaeI	CTAG	1 cut(s)	80
MaeII	ACGT	6 cut(s)	73, 112, 250, 298, 463, 664
MaeIII	GTNAC	2 cut(s)	494, 921
MalI	GATC	8 cut(s)	18, 78, 159, 292, 460, 558, 571, 864
MboI	GATC	8 cut(s)	16, 76, 157, 290, 458, 556, 569, 862
MboII	GAAGA	5 cut(s)	48, 117, 370, 489, 604
MflI	RGATCY	1 cut(s)	290
MhlI	GDGCHC	5 cut(s)	247, 334, 581, 713, 872
MluCI	AATT	4 cut(s)	203, 230, 307, 425
MluI	ACGCGT	1 cut(s)	65
MlyI	GAGTC	3 cut(s)	451, 604, 929
MmeI	TCCRAC	1 cut(s)	436
MseI	TTAA	3 cut(s)	306, 428, 761
MspI	CCGG	1 cut(s)	746
MspR9I	CCNGG	4 cut(s)	288, 727, 757, 786
MvaI	CCWGG	4 cut(s)	288, 727, 757, 786
MvnI	CGCG	4 cut(s)	67, 259, 435, 843
MwoI	GCNNNNNNNGC	5 cut(s)	176, 242, 321, 507, 516
NdeII	GATC	8 cut(s)	16, 76, 157, 290, 458, 556, 569, 862
NlaIII	CATG	1 cut(s)	127
NlaIV	GGNNCC	4 cut(s)	246, 530, 578, 869
NmuCI	GTSAC	1 cut(s)	921
PaeR7I	CTCGAG	1 cut(s)	162
PasI	CCCWGGG	1 cut(s)	726
PcsI	WCGNNNNNNNCGW	2 cut(s)	469, 661
PfoI	TCCNGGA	1 cut(s)	286
PkrI	GCNGC	4 cut(s)	509, 512, 629, 917
Ple19I	CGATCG	1 cut(s)	865
PleI	GAGTC	3 cut(s)	450, 603, 928
PmaCI	CACGTG	2 cut(s)	74, 251
PmlI	CACGTG	2 cut(s)	74, 251
PpsI	GAGTC	3 cut(s)	450, 603, 928
Ppu21I	YACGTR	3 cut(s)	74, 113, 251
PpuMI	RGGWCCY	2 cut(s)	278, 528
PshAI	GACNNNNGTC	1 cut(s)	897
Psp5II	RGGWCCY	2 cut(s)	278, 528
Psp6I	CCWGG	4 cut(s)	286, 725, 755, 784
PspCI	CACGTG	2 cut(s)	74, 251
PspFI	CCCAGC	2 cut(s)	347, 532
PspGI	CCWGG	4 cut(s)	286, 725, 755, 784
PspN4I	GGNNCC	4 cut(s)	246, 530, 578, 869
PspPI	GGNCC	2 cut(s)	278, 528
PspPPI	RGGWCCY	2 cut(s)	278, 528
PsuI	RGATCY	1 cut(s)	290
PvuI	CGATCG	1 cut(s)	865
RsaI	GTAC	1 cut(s)	111
RsaNI	GTAC	1 cut(s)	110
SaqAI	TTAA	3 cut(s)	306, 428, 761
SatI	GCNGC	4 cut(s)	508, 511, 628, 916
Sau3AI	GATC	8 cut(s)	16, 76, 157, 290, 458, 556, 569, 862
Sau96I	GGNCC	2 cut(s)	278, 528
SchI	GAGTC	3 cut(s)	451, 604, 929
ScrFI	CCNGG	4 cut(s)	288, 727, 757, 786
SduI	GDGCHC	5 cut(s)	247, 334, 581, 713, 872
SfaNI	GCATC	2 cut(s)	157, 166
SfcI	CTRYAG	1 cut(s)	514
Sfr274I	CTCGAG	1 cut(s)	162
SinI	GGWCC	2 cut(s)	278, 528
SlaI	CTCGAG	1 cut(s)	162
SmlI	CTYRAG	1 cut(s)	162
SmoI	CTYRAG	1 cut(s)	162
Sse9I	AATT	4 cut(s)	203, 230, 307, 425
SsiI	CCGC	6 cut(s)	130, 259, 435, 439, 508, 843
SspI	AATATT	1 cut(s)	266
SspMI	CTAG	1 cut(s)	80
StyD4I	CCNGG	4 cut(s)	286, 725, 755, 784
TaaI	ACNGT	2 cut(s)	650, 899
TaiI	ACGT	6 cut(s)	76, 115, 253, 301, 466, 667
TaqI	TCGA	2 cut(s)	19, 163
TasI	AATT	4 cut(s)	203, 230, 307, 425
TauI	GCSGC	1 cut(s)	510
Tru1I	TTAA	3 cut(s)	306, 428, 761
Tru9I	TTAA	3 cut(s)	306, 428, 761
TscAI	CASTG	3 cut(s)	332, 410, 606
TseFI	GTSAC	1 cut(s)	921
TseI	GCWGC	3 cut(s)	510, 627, 915
Tsp45I	GTSAC	1 cut(s)	921
TspDTI	ATGAA	1 cut(s)	140
TspGWI	ACGGA	1 cut(s)	589
TspRI	CASTG	3 cut(s)	332, 410, 606
VpaK11BI	GGWCC	2 cut(s)	278, 528
XapI	RAATTY	2 cut(s)	203, 230
XbaI	TCTAGA	1 cut(s)	79
XhoI	CTCGAG	1 cut(s)	162
XmiI	GTMKAC	1 cut(s)	645
XspI	CTAG	1 cut(s)	80

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.