Rw3G013220

serine arginine-rich splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005963
Physical Location & Seq
Forward (+)
13134884 .. 13136724
1841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013220.1

Sequence Viewer

Length: 831 bp
ATGCCTCGCTACGATGATCGATATGGCAATTCGCGTCTGTATGTTGGTCGCCTGGCCTCACGCACCCGGACGCGTGATCTGGACCGGATTTTCAGCAGATATGGGAGAGTACGAGATGTGGACATGAAGCGAGAGTTTGCATTTGTTGAATTTTATGATCCGCGAGATGCTGATGATGCAAGATATAGCTTAGATGGTAGGGAAGTTGATGGTAGTCGTCTGATTGTGGAGTTCGCAAAGGGGGCTCCACGTGGTTCCCGGGAATACATGGGCAGAGGTCCTCCACCTGGATCTGGGCGCTGCTTTAATTGTGGCATTGATGGTCACTGGGCTCGTGATTGTAAGGCTGGGGATTGGAAGAATAAATGCTATCGCTGTGGAGAAAGAGGCCATATAGAAAGGCAGTGCAAAAATAGCCCAAAAGAACTAAGGCGTGAGCGGAGTCGTTCACGTTCTCCTCGCCGCAGAAGTCGTAGCCCAAGTTACAGCCGAAGCTACAGCCGATCAAGGTCCCCGGTCAGGAGAGGACGGAGCTTGGAAAGGGATGATAGATCTCGGAGCCCTTCTTACAGAAGCCGTGATGTGAGCATGAGTCCATTGCCCAGGAAGCGAAGCCTTACACCTGAGGATTATAGCCCCCGTGGAAGGGGTAGCCCTTCTCCTAGAAATGGTAGCAAAAGCCCTAGCCCTAAGAGCAGAAGTCCGATTAGCCCTGACCGCGAGATTGATAGCCCTGTTCGCCCTTCTGAAGCTAATGGTCGTAGCCCCAGTCCAAGGGAGGATAGGAGCCCTGTCGATGAGGATGATTATGACAACCGCCGCTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000245 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0019904 GO:0022607 GO:0022613 GO:0022618 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031503 GO:0031974 GO:0031981 GO:0033036 GO:0033119 GO:0034613 GO:0034622 GO:0034641 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0045934 GO:0046483 GO:0046907 GO:0048024 GO:0048025 GO:0048519 GO:0048523 GO:0050657 GO:0050658 GO:0050684 GO:0050686 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051253 GO:0051641 GO:0051649 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:1901360 GO:1903311 GO:1903312
KEGG Pathways
Metabolic & Signaling
ko03040 ko05168 map03040 map05168
Pfam Domains
Protein Families
RRM_1 zf-CCHC

Protein Analysis

276

Amino Acids

31.96

Weight (kDa)

10.5

Isoelectric Point (pI)

102.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 13 - 74 2.3e-13 RNA recognition motif
zf-CCHC PF00098 100 - 115 2.6e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 439, 822
AccII CGCG 4 cut(s) 34, 73, 163, 720
AciI CCGC 6 cut(s) 161, 439, 463, 718, 817, 820
AclWI GGATC 2 cut(s) 152, 298
AcsI RAATTY 1 cut(s) 149
AcuI CTGAAG 1 cut(s) 768
AcvI CACGTG 1 cut(s) 251
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 7 cut(s) 287, 293, 519, 645, 646, 668, 774
AflIII ACRYGT 1 cut(s) 71
AgsI TTSAA 1 cut(s) 149
AjnI CCWGG 3 cut(s) 51, 286, 602
AluBI AGCT 4 cut(s) 189, 495, 534, 752
AluI AGCT 4 cut(s) 189, 495, 534, 752
AlwI GGATC 2 cut(s) 152, 298
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 2 cut(s) 54, 388
ApeKI GCWGC 1 cut(s) 300
ApoI RAATTY 1 cut(s) 149
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 82, 278, 510
AsuC2I CCSGG 4 cut(s) 67, 259, 260, 515
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 3 cut(s) 82, 278, 510
AxyI CCTNAGG 1 cut(s) 624
BanII GRGCYC 4 cut(s) 247, 334, 563, 791
BauI CACGAG 1 cut(s) 333
BbrPI CACGTG 1 cut(s) 251
BbvI GCAGC 1 cut(s) 287
BccI CCATC 3 cut(s) 188, 203, 314
BceAI ACGGC 1 cut(s) 561
BciT130I CCWGG 3 cut(s) 53, 288, 604
BcnI CCSGG 4 cut(s) 67, 259, 260, 515
BfaI CTAG 2 cut(s) 663, 684
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 1 cut(s) 301
BglII AGATCT 1 cut(s) 551
BisI GCNGC 3 cut(s) 301, 463, 820
BlsI GCNGC 3 cut(s) 302, 464, 821
Bme1390I CCNGG 7 cut(s) 53, 67, 259, 260, 288, 515, 604
Bme18I GGWCC 3 cut(s) 82, 278, 510
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 3 cut(s) 82, 278, 510
BmiI GGNNCC 5 cut(s) 246, 256, 512, 560, 788
BmrFI CCNGG 7 cut(s) 53, 67, 259, 260, 288, 515, 604
BmrI ACTGGG 2 cut(s) 337, 762
BmsI GCATC 2 cut(s) 157, 166
BmuI ACTGGG 2 cut(s) 337, 762
BpuMI CCSGG 4 cut(s) 67, 259, 260, 515
Bsa29I ATCGAT 1 cut(s) 19
BsaAI YACGTR 1 cut(s) 251
BsaJI CCNNGG 5 cut(s) 258, 513, 602, 640, 773
BsaWI WCCGGW 1 cut(s) 84
Bsc4I CCNNNNNNNGG 7 cut(s) 287, 293, 519, 645, 646, 668, 774
Bse1I ACTGG 2 cut(s) 332, 768
Bse21I CCTNAGG 1 cut(s) 624
Bse3DI GCAATG 1 cut(s) 596
BseBI CCWGG 3 cut(s) 53, 288, 604
BseCI ATCGAT 1 cut(s) 19
BseDI CCNNGG 5 cut(s) 258, 513, 602, 640, 773
BseGI GGATG 2 cut(s) 550, 808
BseLI CCNNNNNNNGG 7 cut(s) 287, 293, 519, 645, 646, 668, 774
BseMI GCAATG 1 cut(s) 596
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 2 cut(s) 332, 768
BseRI GAGGAG 1 cut(s) 447
BseXI GCAGC 1 cut(s) 287
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 4 cut(s) 34, 73, 163, 720
BshFI GGCC 2 cut(s) 56, 390
BshVI ATCGAT 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 4 cut(s) 67, 85, 259, 515
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 7 cut(s) 287, 293, 519, 645, 646, 668, 774
BsmFI GGGAC 1 cut(s) 496
BsnI GGCC 2 cut(s) 56, 390
BsoBI CYCGRG 1 cut(s) 258
Bsp1286I GDGCHC 4 cut(s) 247, 334, 563, 791
Bsp143I GATC 6 cut(s) 16, 76, 157, 290, 503, 551
BspACI CCGC 6 cut(s) 161, 439, 463, 718, 817, 820
BspANI GGCC 2 cut(s) 56, 390
BspCNI CTCAG 1 cut(s) 616
BspDI ATCGAT 1 cut(s) 19
BspFNI CGCG 4 cut(s) 34, 73, 163, 720
BspLI GGNNCC 5 cut(s) 246, 256, 512, 560, 788
BspPI GGATC 2 cut(s) 152, 298
BsrBI CCGCTC 2 cut(s) 439, 822
BsrDI GCAATG 1 cut(s) 596
BsrI ACTGG 2 cut(s) 332, 768
BssECI CCNNGG 5 cut(s) 258, 513, 602, 640, 773
BssMI GATC 6 cut(s) 16, 76, 157, 290, 503, 551
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 773
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 3 cut(s) 53, 288, 604
BstBAI YACGTR 1 cut(s) 251
BstDEI CTNAG 4 cut(s) 190, 428, 624, 690
BstDSI CCRYGG 1 cut(s) 640
BstF5I GGATG 2 cut(s) 550, 808
BstFNI CGCG 4 cut(s) 34, 73, 163, 720
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 6 cut(s) 19, 79, 160, 293, 506, 554
BstMBI GATC 6 cut(s) 16, 76, 157, 290, 503, 551
BstMWI GCNNNNNNNGC 7 cut(s) 176, 242, 414, 607, 693, 717, 738
BstNI CCWGG 3 cut(s) 53, 288, 604
BstSCI CCNGG 7 cut(s) 51, 65, 257, 258, 286, 513, 602
BstSFI CTRYAG 1 cut(s) 496
BstUI CGCG 4 cut(s) 34, 73, 163, 720
BstV1I GCAGC 1 cut(s) 287
BstX2I RGATCY 2 cut(s) 290, 551
BstYI RGATCY 2 cut(s) 290, 551
Bsu15I ATCGAT 1 cut(s) 19
Bsu36I CCTNAGG 1 cut(s) 624
BsuRI GGCC 2 cut(s) 56, 390
BsuTUI ATCGAT 1 cut(s) 19
BtgI CCRYGG 1 cut(s) 640
BtsCI GGATG 2 cut(s) 550, 808
BtsI GCAGTG 1 cut(s) 410
BtsIMutI CAGTG 2 cut(s) 325, 410
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 3 cut(s) 82, 278, 510
Cfr9I CCCGGG 1 cut(s) 258
ClaI ATCGAT 1 cut(s) 19
CseI GACGC 2 cut(s) 23, 79
Csp6I GTAC 1 cut(s) 110
CviAII CATG 3 cut(s) 124, 268, 589
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 4 cut(s) 190, 428, 624, 690
DpnI GATC 6 cut(s) 18, 78, 159, 292, 505, 553
DpnII GATC 6 cut(s) 16, 76, 157, 290, 503, 551
Eco130I CCWWGG 1 cut(s) 773
Eco24I GRGCYC 4 cut(s) 247, 334, 563, 791
Eco47I GGWCC 3 cut(s) 82, 278, 510
Eco57I CTGAAG 1 cut(s) 768
Eco72I CACGTG 1 cut(s) 251
Eco81I CCTNAGG 1 cut(s) 624
Eco88I CYCGRG 1 cut(s) 258
EcoO109I RGGNCCY 2 cut(s) 278, 510
EcoRII CCWGG 3 cut(s) 51, 286, 602
EcoT14I CCWWGG 1 cut(s) 773
EcoT38I GRGCYC 4 cut(s) 247, 334, 563, 791
ErhI CCWWGG 1 cut(s) 773
FaeI CATG 3 cut(s) 127, 271, 592
FaqI GGGAC 1 cut(s) 496
FatI CATG 3 cut(s) 123, 267, 588
Fnu4HI GCNGC 3 cut(s) 301, 463, 820
FokI GGATG 2 cut(s) 557, 815
FriOI GRGCYC 4 cut(s) 247, 334, 563, 791
Fsp4HI GCNGC 3 cut(s) 301, 463, 820
FspBI CTAG 2 cut(s) 663, 684
GlaI GCGC 1 cut(s) 299
GluI GCNGC 3 cut(s) 301, 463, 820
GsaI CCCAGC 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 2 cut(s) 56, 390
HapII CCGG 4 cut(s) 67, 85, 259, 515
HgaI GACGC 2 cut(s) 23, 79
HhaI GCGC 1 cut(s) 300
Hin1II CATG 3 cut(s) 127, 271, 592
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HinfI GANTC 2 cut(s) 442, 592
HpaII CCGG 4 cut(s) 67, 85, 259, 515
Hpy166II GTNNAC 2 cut(s) 121, 449
Hpy188I TCNGA 4 cut(s) 222, 558, 705, 748
Hpy188III TCNNGA 3 cut(s) 80, 335, 520
Hpy8I GTNNAC 2 cut(s) 121, 449
HpyAV CCTTC 4 cut(s) 573, 639, 666, 753
HpyCH4IV ACGT 2 cut(s) 250, 451
HpyCH4V TGCA 3 cut(s) 140, 179, 408
HpyF10VI GCNNNNNNNGC 7 cut(s) 176, 242, 414, 607, 693, 717, 738
HpyF3I CTNAG 4 cut(s) 190, 428, 624, 690
HpySE526I ACGT 2 cut(s) 250, 451
Hsp92II CATG 3 cut(s) 127, 271, 592
HspAI GCGC 1 cut(s) 298
Kzo9I GATC 6 cut(s) 16, 76, 157, 290, 503, 551
LmnI GCTCC 4 cut(s) 250, 531, 558, 786
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 2 cut(s) 157, 166
MaeI CTAG 2 cut(s) 663, 684
MaeII ACGT 2 cut(s) 250, 451
MaeIII GTNAC 2 cut(s) 323, 482
MalI GATC 6 cut(s) 18, 78, 159, 292, 505, 553
MbiI CCGCTC 2 cut(s) 439, 822
MboI GATC 6 cut(s) 16, 76, 157, 290, 503, 551
MboII GAAGA 1 cut(s) 370
MflI RGATCY 2 cut(s) 290, 551
MhlI GDGCHC 4 cut(s) 247, 334, 563, 791
MluCI AATT 3 cut(s) 28, 149, 307
MluI ACGCGT 1 cut(s) 71
MlyI GAGTC 2 cut(s) 451, 601
MseI TTAA 1 cut(s) 306
MspI CCGG 4 cut(s) 67, 85, 259, 515
MspR9I CCNGG 7 cut(s) 53, 67, 259, 260, 288, 515, 604
MvaI CCWGG 3 cut(s) 53, 288, 604
MvnI CGCG 4 cut(s) 34, 73, 163, 720
MwoI GCNNNNNNNGC 7 cut(s) 176, 242, 414, 607, 693, 717, 738
NciI CCSGG 4 cut(s) 67, 259, 260, 515
NdeII GATC 6 cut(s) 16, 76, 157, 290, 503, 551
NlaIII CATG 3 cut(s) 127, 271, 592
NlaIV GGNNCC 5 cut(s) 246, 256, 512, 560, 788
NmuCI GTSAC 1 cut(s) 323
PkrI GCNGC 3 cut(s) 302, 464, 821
PleI GAGTC 2 cut(s) 450, 600
PmaCI CACGTG 1 cut(s) 251
PmlI CACGTG 1 cut(s) 251
PpsI GAGTC 2 cut(s) 450, 600
Ppu21I YACGTR 1 cut(s) 251
PpuMI RGGWCCY 2 cut(s) 278, 510
Psp5II RGGWCCY 2 cut(s) 278, 510
Psp6I CCWGG 3 cut(s) 51, 286, 602
PspCI CACGTG 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 3 cut(s) 51, 286, 602
PspN4I GGNNCC 5 cut(s) 246, 256, 512, 560, 788
PspPI GGNCC 3 cut(s) 82, 278, 510
PspPPI RGGWCCY 2 cut(s) 278, 510
PsuI RGATCY 2 cut(s) 290, 551
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 3 cut(s) 301, 463, 820
Sau3AI GATC 6 cut(s) 16, 76, 157, 290, 503, 551
Sau96I GGNCC 3 cut(s) 82, 278, 510
SchI GAGTC 2 cut(s) 451, 601
ScrFI CCNGG 7 cut(s) 53, 67, 259, 260, 288, 515, 604
SduI GDGCHC 4 cut(s) 247, 334, 563, 791
SfaNI GCATC 2 cut(s) 157, 166
SfcI CTRYAG 1 cut(s) 496
SinI GGWCC 3 cut(s) 82, 278, 510
SmaI CCCGGG 1 cut(s) 260
Sse9I AATT 3 cut(s) 28, 149, 307
SsiI CCGC 6 cut(s) 161, 439, 463, 718, 817, 820
SspMI CTAG 2 cut(s) 663, 684
StyD4I CCNGG 7 cut(s) 51, 65, 257, 258, 286, 513, 602
StyI CCWWGG 1 cut(s) 773
TaiI ACGT 2 cut(s) 253, 454
TaqI TCGA 2 cut(s) 19, 795
TasI AATT 3 cut(s) 28, 149, 307
TauI GCSGC 2 cut(s) 465, 822
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 2 cut(s) 332, 410
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 1 cut(s) 140
TspGWI ACGGA 1 cut(s) 544
TspMI CCCGGG 1 cut(s) 258
TspRI CASTG 2 cut(s) 332, 410
VpaK11BI GGWCC 3 cut(s) 82, 278, 510
XapI RAATTY 1 cut(s) 149
XmaI CCCGGG 1 cut(s) 258
XspI CTAG 2 cut(s) 663, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.