MD12G1168300.v1.1
MYB Family

Trihelix transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013088
Physical Location & Seq
Forward (+)
24873784 .. 24875513
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1168300.v1.1.491

Sequence Viewer

Length: 891 bp
ATGGAACATCATCCTCATCATTTCCAACATCACCACCAGCAGCAACATCATCCCCAGCAGCTTCAGCTTCAACAACAACAGCAGCAGCAGCAGCAGCTTCAACAGCAGCATCCTAGTACTGCAACTGTACACGTGGACGCGAGTGATAGGTTTCCTCAATGGAGCGTTCAGGAGACGAAGGACTTGCTTCTGATCAGAGCCGAGCTCGATCAGACTTTTATGGAGACGAAGAGAAACAAGCTTCTGTGGGAAGTGATTTCTACGAAGATGAAGGAAAAGGGTCACCATCGCAGCGCAGAACAGTGCAAGTGCAAGTGGAAAAACCTCGTTACTCGTTACAAGGGTTGTGAGACAATGGAGCCGGAACCTATGAGGCAGCAATTCCCGTTTTACAATGAGCTACAAGCGATTTTCGCTGCACGGATCCAGAGAATGCTGTGGGCAGAAGAAAGCGGAGCTGCAAGCGGGTCGAAGAAGAAAACGCTTTCTTCAGACGATGAGGAAGATAACGAGGATAGCGAGGCAGAGAATAAGGGAAGCACGGCGAGGAACAAAAAGAAAAAGGTGAAGATGAGCAATATCGGAAGCAGCGGTACTAGTGGGAGTGCAAGTGGAAATAATGTGAGGGAGATATTGGAGGGGTTCATGAAGCAACAAATGCAAATGGAAATGCAATGGAGGGAAGAGTTTCAAGCAAGGGAGAACGAGAGGCGGTTAAAGGAGATGGAGTGGAGGCAGAGGATGGAAGCGTTGGAGAACGAGAGGATAATGATGGAGACGAGATGGAGGGAGAGGGAGGAGCAAAGGAGATTGAGGGAGGAGGCTAGGGCCGAGAAGAGGGATGCTCTTATAACTGCTTTGCTAAACAAGCTTAGAAGAGAGGATATGTAG

Protein Analysis

297

Amino Acids

35.42

Weight (kDa)

8.35

Isoelectric Point (pI)

63.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 53 - 137 1.6e-21 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38250
fragaria_vesca FvH4_6g11330 FvH4_6g11330
malus_domestica MD04G1154600.v1.1 MD12G1168300.v1.1
prunus_persica Prupe.6G278600_v2.0.a1
pyrus_communis pycom04g13960 pycom12g15980
rosa_chinensis RchiOBHm_Chr3g0462911
rosa_laevigata RLG00000024825
rosa_multiflora Rmu_sc0000766.1_g000017
rosa_roxburghii Rroxscaffold_6G00417110
rosa_rugosa Rorug03G0059700
rosa_samantha Rh3AG118000 Rh3BG122900 Rh3CG124700 Rh3DG123400
rosa_wichuraiana Rw3G010010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 851
AccII CGCG 1 cut(s) 140
AciI CCGC 4 cut(s) 453, 465, 591, 712
AclWI GGATC 2 cut(s) 418, 431
AcuI CTGAAG 2 cut(s) 47, 474
AcvI CACGTG 1 cut(s) 133
AfaI GTAC 3 cut(s) 118, 129, 595
AfiI CCNNNNNNNGG 1 cut(s) 837
AflIII ACRYGT 1 cut(s) 130
AgsI TTSAA 3 cut(s) 71, 101, 692
AhlI ACTAGT 1 cut(s) 596
AluBI AGCT 8 cut(s) 61, 67, 97, 205, 241, 400, 458, 871
AluI AGCT 8 cut(s) 61, 67, 97, 205, 241, 400, 458, 871
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 4 cut(s) 167, 218, 344, 770
AlwI GGATC 2 cut(s) 418, 431
AoxI GGCC 1 cut(s) 828
Asp700I GAANNNNTTC 1 cut(s) 687
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 828
AsuHPI GGTGA 3 cut(s) 23, 275, 577
BamHI GGATCC 1 cut(s) 423
BanII GRGCYC 1 cut(s) 207
BarI GAAGNNNNNNTAC 2 cut(s) 577, 609
BbrPI CACGTG 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 207
BccI CCATC 5 cut(s) 294, 718, 736, 766, 777
BceAI ACGGC 1 cut(s) 558
BcgI CGANNNNNNTGC 4 cut(s) 166, 200, 450, 484
BclI TGATCA 1 cut(s) 192
BcoDI GTCTC 4 cut(s) 167, 218, 344, 770
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 3 cut(s) 114, 597, 825
BmcAI AGTACT 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 828
BmiI GGNNCC 3 cut(s) 360, 366, 425
BmsI GCATC 2 cut(s) 118, 832
BplI GAGNNNNNCTC 4 cut(s) 189, 221, 829, 861
BsaAI YACGTR 1 cut(s) 133
BsaXI ACNNNNNCTCC 4 cut(s) 595, 625, 713, 743
Bsc4I CCNNNNNNNGG 1 cut(s) 837
Bse3DI GCAATG 1 cut(s) 680
BseGI GGATG 5 cut(s) 10, 49, 109, 747, 847
BseLI CCNNNNNNNGG 1 cut(s) 837
BseMI GCAATG 1 cut(s) 680
BseRI GAGGAG 2 cut(s) 812, 833
BseYI CCCAGC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 402
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 1 cut(s) 830
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 837
BsmAI GTCTC 4 cut(s) 167, 218, 344, 770
BsmBI CGTCTC 3 cut(s) 167, 218, 770
BsmI GAATGC 1 cut(s) 438
BsnI GGCC 1 cut(s) 830
Bsp1286I GDGCHC 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 127
Bsp143I GATC 3 cut(s) 192, 208, 423
BspACI CCGC 4 cut(s) 453, 465, 591, 712
BspANI GGCC 1 cut(s) 830
BspFNI CGCG 1 cut(s) 140
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 3 cut(s) 360, 366, 425
BspPI GGATC 2 cut(s) 418, 431
BsrDI GCAATG 1 cut(s) 680
BsrGI TGTACA 1 cut(s) 127
BssMI GATC 3 cut(s) 192, 208, 423
Bst4CI ACNGT 2 cut(s) 127, 303
Bst6I CTCTTC 4 cut(s) 224, 678, 830, 871
BstAPI GCANNNNNTGC 1 cut(s) 658
BstAUI TGTACA 1 cut(s) 127
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 463
BstDEI CTNAG 1 cut(s) 872
BstEII GGTNACC 1 cut(s) 281
BstF5I GGATG 5 cut(s) 10, 49, 109, 747, 847
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 3 cut(s) 195, 211, 426
BstMAI GTCTC 4 cut(s) 167, 218, 344, 770
BstMBI GATC 3 cut(s) 192, 208, 423
BstMWI GCNNNNNNNGC 8 cut(s) 64, 88, 91, 94, 103, 413, 658, 868
BstPI GGTNACC 1 cut(s) 281
BstUI CGCG 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 423
BstYI RGATCY 1 cut(s) 423
BsuRI GGCC 1 cut(s) 830
BtgZI GCGATG 1 cut(s) 272
BtsCI GGATG 5 cut(s) 10, 49, 109, 747, 847
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 463
CciI TCATGA 1 cut(s) 645
CfoI GCGC 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 828
CseI GACGC 1 cut(s) 146
Csp6I GTAC 3 cut(s) 117, 128, 594
CviAII CATG 1 cut(s) 646
CviQI GTAC 3 cut(s) 117, 128, 594
DdeI CTNAG 1 cut(s) 872
DpnI GATC 3 cut(s) 194, 210, 425
DpnII GATC 3 cut(s) 192, 208, 423
Eam1104I CTCTTC 4 cut(s) 224, 678, 830, 871
EarI CTCTTC 4 cut(s) 224, 678, 830, 871
Ecl136II GAGCTC 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 2 cut(s) 47, 474
Eco72I CACGTG 1 cut(s) 133
Eco91I GGTNACC 1 cut(s) 281
EcoICRI GAGCTC 1 cut(s) 205
EcoO65I GGTNACC 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 207
Esp3I CGTCTC 3 cut(s) 167, 218, 770
FaeI CATG 1 cut(s) 649
FaiI YATR 5 cut(s) 221, 371, 647, 851, 887
FatI CATG 1 cut(s) 645
FauI CCCGC 1 cut(s) 458
FbaI TGATCA 1 cut(s) 192
FokI GGATG 4 cut(s) 36, 96, 754, 854
FriOI GRGCYC 1 cut(s) 207
FspBI CTAG 3 cut(s) 114, 597, 825
GlaI GCGC 1 cut(s) 295
GsaI CCCAGC 1 cut(s) 58
HaeIII GGCC 1 cut(s) 830
HapII CCGG 1 cut(s) 362
HgaI GACGC 1 cut(s) 146
HhaI GCGC 1 cut(s) 296
Hin1II CATG 1 cut(s) 649
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HindIII AAGCTT 2 cut(s) 239, 869
HpaII CCGG 1 cut(s) 362
HphI GGTGA 3 cut(s) 23, 275, 577
Hpy166II GTNNAC 2 cut(s) 130, 136
Hpy188I TCNGA 5 cut(s) 192, 197, 213, 493, 584
Hpy188III TCNNGA 3 cut(s) 170, 427, 646
Hpy8I GTNNAC 2 cut(s) 130, 136
HpyAV CCTTC 2 cut(s) 172, 265
HpyCH4III ACNGT 2 cut(s) 127, 303
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 8 cut(s) 122, 306, 312, 419, 461, 608, 661, 673
HpyF10VI GCNNNNNNNGC 8 cut(s) 64, 88, 91, 94, 103, 413, 658, 868
HpyF3I CTNAG 1 cut(s) 872
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 1 cut(s) 649
HspAI GCGC 1 cut(s) 294
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 3 cut(s) 192, 208, 423
LmnI GCTCC 4 cut(s) 162, 358, 455, 799
LpnPI CCDG 5 cut(s) 50, 68, 155, 375, 440
LweI GCATC 2 cut(s) 118, 832
MaeI CTAG 3 cut(s) 114, 597, 825
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 281, 328, 335
MalI GATC 3 cut(s) 194, 210, 425
MboI GATC 3 cut(s) 192, 208, 423
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 1 cut(s) 380
MmeI TCCRAC 2 cut(s) 49, 732
MroXI GAANNNNTTC 1 cut(s) 687
MseI TTAA 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 591
MspI CCGG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 438
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 8 cut(s) 64, 88, 91, 94, 103, 413, 658, 868
NdeII GATC 3 cut(s) 192, 208, 423
NlaIII CATG 1 cut(s) 649
NlaIV GGNNCC 3 cut(s) 360, 366, 425
NmeAIII GCCGAG 2 cut(s) 226, 856
NmuCI GTSAC 1 cut(s) 281
PagI TCATGA 1 cut(s) 645
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PctI GAATGC 1 cut(s) 438
PdmI GAANNNNTTC 1 cut(s) 687
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
Ppu21I YACGTR 1 cut(s) 133
PsiI TTATAA 1 cut(s) 851
Psp124BI GAGCTC 1 cut(s) 207
PspCI CACGTG 1 cut(s) 133
PspEI GGTNACC 1 cut(s) 281
PspFI CCCAGC 1 cut(s) 54
PspN4I GGNNCC 3 cut(s) 360, 366, 425
PspPI GGNCC 1 cut(s) 828
PsuI RGATCY 1 cut(s) 423
RsaI GTAC 3 cut(s) 118, 129, 595
RsaNI GTAC 3 cut(s) 117, 128, 594
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 716
Sau3AI GATC 3 cut(s) 192, 208, 423
Sau96I GGNCC 1 cut(s) 828
ScaI AGTACT 1 cut(s) 118
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 2 cut(s) 118, 832
SpeI ACTAGT 1 cut(s) 596
Sse9I AATT 1 cut(s) 380
SsiI CCGC 4 cut(s) 453, 465, 591, 712
SspMI CTAG 3 cut(s) 114, 597, 825
SstI GAGCTC 1 cut(s) 207
TaaI ACNGT 2 cut(s) 127, 303
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 207, 470
TasI AATT 1 cut(s) 380
TatI WGTACW 2 cut(s) 116, 127
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TscAI CASTG 1 cut(s) 308
TseFI GTSAC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 3 cut(s) 284, 634, 662
TspGWI ACGGA 1 cut(s) 436
TspRI CASTG 1 cut(s) 308
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 3 cut(s) 114, 597, 825
ZrmI AGTACT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.