Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006051

Physical Location & Seq

Genomic Coordinates Forward (+)

25265610 .. 25269648

Gene Structure 4039 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1172300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGGAGAACCGGTTCGCAGTCTACATCTTCGTGTCGGCGCTGCTCTTCCTCTTAACCATGGAAGCGAAAACTATAGACCCTTACAAGGTCCTTGGAGTGGAGCGGAATGCAAGTCAGCGTGAAATTCAGAAAGCGTTCCACAAGCTCTCTCTTCAATATCACCCAGACAAGAATAAAAGCAAGGGAGCTCAAGCAAAGTTTGCTGAGATAAACAATGCATATGAGATTTTATCTGATGAAGAGAAGAGGAAAAATTATGATATGTATGGAGATGAAAAGGGAAATCCTGGATTTGGAGCTGGTTCCCCTGAAGATCATGGTGAATATACTTACTTCACAAATGGTGGACCAGGTCAGAACCACTTTACCTATGGACCTGGTGACTGGCAGAGCATGGGTGGGCAGGGAGGTTCCAAGTCATTCTCTTTTTCCTTTGGAGGTCCCAGTGGTCCAAGTTCATTTAGTTTTGGTATGGAAGACATTTTCTCAAACTTTTTTGGGGGTAAACCTGGAGGTGGGGGTCAGTTTGATGGTTTCAGTGGTTCAACTGGGTCTCGACCTGGTTCTCGGCCTGGGTCTAGGACTTCTCCGGTGAGCATTACAACCATCAGTCCACAGGTGTATAAGAAAGAAATAGTTCACCGAGGGATGACTTGGCTCCTGTTCTCTTACACTCCTTCATTGAAAGGGAAGCAACATGTTGAAAATATCATAGAGGAAGTTGCGAGTTCCCTGCAGGGAGCCTTAAAGGTTGGAAGCATAAACTGTGAGACGGAACCATCTCTCTGCAAGGACCTTGGCATATACCCCAGCAGAATGCCCAGAGTATTTGTGTACAAAGTGAGTGAAAAGGGTTCTTTGGTTGAGTATGATGGTGATTGGGCTGCAAAACCTTTGAAGTCATTTTGCCAAGACAATCTGCCAAGGTTTTCGAAACGGGTTGACTTGAACCGCTTCGAGTCTTCCACTTTTACAACCAATAAATTACCTAGTGTGGTGCTTCTTTCCACCAAGAAAGATACACCTGTTATCTGGCGTGTCCTCAGTGGATTGCATCACAATCACTTCAGTTTCTATGATGCAGAGGTCCACGATGCTTCTGTTCCAACAGTGAAGAAGCTGGGAGTTGGTGCACTTCCAGCTATAGTTGGTTGGCTTCCGAACGGGGAGAAGCATGTCTTAAAAGCAGGCATTACCACCAAAGATATGAAGTCTACAATTAAGGAGCTCAGTGCTTTACTCGAAGGTTTTGAAAAAAAGAGCAAGAAGGCAGCTTCAAGTCAGGCCAAGAAATCACCGACTGATTCGGGGGAGAATCAAGTACCTCTATTGACACATTCTAATTTTGATGCCCTTTGTGGTGAGAAAACTCCAGTTTGCATCATTGGCGCATTCAGATCTTCCAAAGCAAGAAATACGCTGGAATCAATTTTGACTGCGGTCTCTCGGAAATCGCTATCAAGGCGACAGAGTTCAGCTCAGGGCAAGGATTCTGTTTCCTACACTCTCTTGGATGCCGCCAAGCAGGCGACCTTCTTAAATGCTTTCGACGAAGCAGGATTTCAATCATTAGAGAAGGTCTTGGTTGCCTACAAACCTCGGAGGGGGACATTCGCGGCATTTGGCGGTGAAATGACTACAGAAGAAGTAGAGGGGTTTATTAGCTCTGTGCTTAACGGGGACATACATTTTACCAAAACACGGCAGAAACCCGTGCTCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005788 GO:0005911 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009506 GO:0009628 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0016491 GO:0022414 GO:0030054 GO:0030154 GO:0031974 GO:0032501 GO:0032502 GO:0032989 GO:0033554 GO:0034605 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050896 GO:0051704 GO:0051716 GO:0055044 GO:0055114 GO:0060560 GO:0070013 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ

Protein Analysis

575

Amino Acids

62.83

Weight (kDa)

9.19

Isoelectric Point (pI)

39.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	26 - 87	1.2e-26	DnaJ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08970 AT3G08970
fragaria_vesca	FvH4_6g11110 FvH4_6g11110 FvH4_6g11110 FvH4_6g11110 FvH4_6g11110 FvH4_6g11110
malus_domestica	MD00G1062300.v1.1 MD12G1172300.v1.1
prunus_persica	Prupe.6G280600_v2.0.a1 Prupe.6G280600_v2.0.a1
pyrus_communis	pycom04g14320 pycom12g16350
rosa_chinensis	RchiOBHm_Chr3g0462541
rosa_laevigata	RLG00000024850
rosa_multiflora	Rmu_co8373099.1_g000001 Rmu_sc0003624.1_g000017 Rmu_sc0023157.1_g000002
rosa_roxburghii	Rroxscaffold_6G00417410 Rroxscaffold_6G00417430
rosa_rugosa	Rorug03G0057500
rosa_samantha	Rh3AG115400 Rh3BG118800 Rh3CG122200 Rh3DG120900
rosa_wichuraiana	Rw3G009770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	103
AccI	GTMKAC	2 cut(s)	21, 1214
AccII	CGCG	1 cut(s)	1616
AciI	CCGC	6 cut(s)	103, 952, 1439, 1518, 1616, 1626
AcsI	RAATTY	1 cut(s)	123
AcuI	CTGAAG	2 cut(s)	330, 1051
AfaI	GTAC	2 cut(s)	836, 1323
AfiI	CCNNNNNNNGG	7 cut(s)	85, 97, 293, 515, 1604, 1701, 1719
AflIII	ACRYGT	1 cut(s)	697
AgeI	ACCGGT	1 cut(s)	9
AgsI	TTSAA	9 cut(s)	155, 546, 685, 704, 898, 949, 1253, 1278, 1565
AjnI	CCWGG	6 cut(s)	286, 349, 376, 508, 559, 571
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	9 cut(s)	145, 188, 299, 1120, 1142, 1228, 1274, 1478, 1665
AluI	AGCT	9 cut(s)	145, 188, 299, 1120, 1142, 1228, 1274, 1478, 1665
Alw21I	GWGCWC	4 cut(s)	190, 1135, 1230, 1719
Alw26I	GTCTC	3 cut(s)	558, 764, 1447
Alw44I	GTGCAC	1 cut(s)	1131
AoxI	GGCC	2 cut(s)	569, 1284
ApaLI	GTGCAC	1 cut(s)	1131
ApeKI	GCWGC	3 cut(s)	40, 884, 1271
ApoI	RAATTY	1 cut(s)	123
AsiGI	ACCGGT	1 cut(s)	9
Asp700I	GAANNNNTTC	4 cut(s)	11, 134, 636, 953
AspLEI	GCGC	2 cut(s)	40, 1391
AspS9I	GGNCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
AsuHPI	GGTGA	9 cut(s)	152, 332, 392, 604, 632, 887, 1287, 1373, 1640
AsuII	TTCGAA	1 cut(s)	932
AvaII	GGWCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
BaeGI	GKGCMC	1 cut(s)	1135
BaeI	ACNNNNGTAYC	2 cut(s)	1011, 1044
BanII	GRGCYC	2 cut(s)	190, 1230
BbsI	GAAGAC	2 cut(s)	483, 954
Bbv12I	GWGCWC	4 cut(s)	190, 1135, 1230, 1719
BbvI	GCAGC	3 cut(s)	27, 871, 1283
BccI	CCATC	4 cut(s)	524, 614, 787, 866
BceAI	ACGGC	1 cut(s)	1718
BcgI	CGANNNNNNTGC	2 cut(s)	715, 749
BciT130I	CCWGG	6 cut(s)	288, 351, 378, 510, 561, 573
BcoDI	GTCTC	3 cut(s)	558, 764, 1447
BfaI	CTAG	2 cut(s)	579, 990
BfmI	CTRYAG	4 cut(s)	72, 734, 1143, 1638
BfoI	RGCGCY	1 cut(s)	41
BglI	GCCNNNNNGGC	1 cut(s)	1526
BglII	AGATCT	1 cut(s)	1397
BisI	GCNGC	5 cut(s)	41, 885, 1272, 1518, 1617
BlsI	GCNGC	5 cut(s)	42, 886, 1273, 1519, 1618
Bme1390I	CCNGG	6 cut(s)	288, 351, 378, 510, 561, 573
Bme18I	GGWCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
BmgT120I	GGNCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
BmiI	GGNNCC	6 cut(s)	304, 412, 442, 659, 742, 777
BmrFI	CCNGG	6 cut(s)	288, 351, 378, 510, 561, 573
BmrI	ACTGGG	2 cut(s)	438, 558
BmsI	GCATC	6 cut(s)	1063, 1069, 1084, 1339, 1389, 1504
BmuI	ACTGGG	2 cut(s)	438, 558
BoxI	GACNNNNGTC	1 cut(s)	1439
BpiI	GAAGAC	2 cut(s)	483, 954
BplI	GAGNNNNNCTC	2 cut(s)	1462, 1494
BpmI	CTGGAG	2 cut(s)	531, 1356
Bpu10I	CCTNAGC	2 cut(s)	1479, 1718
Bpu14I	TTCGAA	1 cut(s)	932
BpuEI	CTTGAG	1 cut(s)	174
BsaBI	GATNNNNATC	1 cut(s)	1564
BsaI	GGTCTC	2 cut(s)	558, 1447
BsaJI	CCNNGG	7 cut(s)	57, 91, 572, 643, 796, 923, 1598
BsaWI	WCCGGW	2 cut(s)	9, 589
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	7 cut(s)	85, 97, 293, 515, 1604, 1701, 1719
Bse118I	RCCGGY	1 cut(s)	9
Bse1I	ACTGG	4 cut(s)	389, 444, 553, 1373
Bse8I	GATNNNNATC	1 cut(s)	1564
BseBI	CCWGG	6 cut(s)	288, 351, 378, 510, 561, 573
BseDI	CCNNGG	7 cut(s)	57, 91, 572, 643, 796, 923, 1598
BseGI	GGATG	2 cut(s)	654, 1519
BseJI	GATNNNNATC	1 cut(s)	1564
BseLI	CCNNNNNNNGG	7 cut(s)	85, 97, 293, 515, 1604, 1701, 1719
BseMII	CTCAG	4 cut(s)	195, 1057, 1243, 1493
BseNI	ACTGG	4 cut(s)	389, 444, 553, 1373
BseSI	GKGCMC	1 cut(s)	1135
BseXI	GCAGC	3 cut(s)	27, 871, 1283
BseYI	CCCAGC	2 cut(s)	809, 1120
Bsh1236I	CGCG	1 cut(s)	1616
BshFI	GGCC	2 cut(s)	571, 1286
BshTI	ACCGGT	1 cut(s)	9
BsiHKAI	GWGCWC	4 cut(s)	190, 1135, 1230, 1719
BsiSI	CCGG	2 cut(s)	10, 590
BslFI	GGGAC	3 cut(s)	426, 1621, 1694
BslI	CCNNNNNNNGG	7 cut(s)	85, 97, 293, 515, 1604, 1701, 1719
BsmAI	GTCTC	3 cut(s)	558, 764, 1447
BsmBI	CGTCTC	1 cut(s)	764
BsmFI	GGGAC	3 cut(s)	426, 1621, 1694
BsmI	GAATGC	3 cut(s)	112, 822, 1391
BsnI	GGCC	2 cut(s)	571, 1286
Bso31I	GGTCTC	2 cut(s)	558, 1447
Bsp119I	TTCGAA	1 cut(s)	932
Bsp1286I	GDGCHC	4 cut(s)	190, 1135, 1230, 1719
Bsp1407I	TGTACA	1 cut(s)	834
Bsp143I	GATC	2 cut(s)	313, 1397
Bsp19I	CCATGG	1 cut(s)	57
BspACI	CCGC	6 cut(s)	103, 952, 1439, 1518, 1616, 1626
BspANI	GGCC	2 cut(s)	571, 1286
BspCNI	CTCAG	4 cut(s)	196, 1056, 1242, 1492
BspFNI	CGCG	1 cut(s)	1616
BspLI	GGNNCC	6 cut(s)	304, 412, 442, 659, 742, 777
BspMAI	CTGCAG	1 cut(s)	738
BspQI	GCTCTTC	1 cut(s)	50
BspT104I	TTCGAA	1 cut(s)	932
BspTNI	GGTCTC	2 cut(s)	558, 1447
BsrBI	CCGCTC	1 cut(s)	103
BsrFI	RCCGGY	1 cut(s)	9
BsrGI	TGTACA	1 cut(s)	834
BsrI	ACTGG	4 cut(s)	389, 444, 553, 1373
BssAI	RCCGGY	1 cut(s)	9
BssECI	CCNNGG	7 cut(s)	57, 91, 572, 643, 796, 923, 1598
BssMI	GATC	2 cut(s)	313, 1397
BssT1I	CCWWGG	4 cut(s)	57, 91, 796, 923
Bst2UI	CCWGG	6 cut(s)	288, 351, 378, 510, 561, 573
Bst4CI	ACNGT	2 cut(s)	767, 1111
Bst6I	CTCTTC	4 cut(s)	50, 156, 234, 239
BstAPI	GCANNNNNTGC	1 cut(s)	200
BstAUI	TGTACA	1 cut(s)	834
BstBI	TTCGAA	1 cut(s)	932
BstC8I	GCNNGC	2 cut(s)	1189, 1527
BstDEI	CTNAG	5 cut(s)	204, 1043, 1229, 1479, 1718
BstDSI	CCRYGG	1 cut(s)	57
BstF5I	GGATG	2 cut(s)	654, 1519
BstFNI	CGCG	1 cut(s)	1616
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	2 cut(s)	40, 1391
BstKTI	GATC	2 cut(s)	316, 1400
BstMAI	GTCTC	3 cut(s)	558, 764, 1447
BstMBI	GATC	2 cut(s)	313, 1397
BstMWI	GCNNNNNNNGC	5 cut(s)	200, 1139, 1386, 1462, 1526
BstNI	CCWGG	6 cut(s)	288, 351, 378, 510, 561, 573
BstNSI	RCATGY	2 cut(s)	701, 1178
BstPAI	GACNNNNGTC	1 cut(s)	1439
BstSCI	CCNGG	6 cut(s)	286, 349, 376, 508, 559, 571
BstSFI	CTRYAG	4 cut(s)	72, 734, 1143, 1638
BstSLI	GKGCMC	1 cut(s)	1135
BstUI	CGCG	1 cut(s)	1616
BstV1I	GCAGC	3 cut(s)	27, 871, 1283
BstV2I	GAAGAC	2 cut(s)	483, 954
BstX2I	RGATCY	1 cut(s)	1397
BstYI	RGATCY	1 cut(s)	1397
BsuRI	GGCC	2 cut(s)	571, 1286
BtgI	CCRYGG	1 cut(s)	57
BtsCI	GGATG	2 cut(s)	654, 1519
BtsIMutI	CAGTG	5 cut(s)	451, 544, 1051, 1116, 1237
Cac8I	GCNNGC	2 cut(s)	1189, 1527
CfoI	GCGC	2 cut(s)	40, 1391
Cfr10I	RCCGGY	1 cut(s)	9
Cfr13I	GGNCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
CsiI	ACCWGGT	3 cut(s)	349, 376, 559
Csp6I	GTAC	2 cut(s)	835, 1322
CspAI	ACCGGT	1 cut(s)	9
CviAII	CATG	5 cut(s)	58, 317, 394, 698, 1175
CviQI	GTAC	2 cut(s)	835, 1322
DdeI	CTNAG	5 cut(s)	204, 1043, 1229, 1479, 1718
DpnI	GATC	2 cut(s)	315, 1399
DpnII	GATC	2 cut(s)	313, 1397
Eam1104I	CTCTTC	4 cut(s)	50, 156, 234, 239
EarI	CTCTTC	4 cut(s)	50, 156, 234, 239
Ecl136II	GAGCTC	2 cut(s)	188, 1228
Eco130I	CCWWGG	4 cut(s)	57, 91, 796, 923
Eco24I	GRGCYC	2 cut(s)	190, 1230
Eco31I	GGTCTC	2 cut(s)	558, 1447
Eco47I	GGWCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
Eco53kI	GAGCTC	2 cut(s)	188, 1228
Eco57I	CTGAAG	2 cut(s)	330, 1051
EcoICRI	GAGCTC	2 cut(s)	188, 1228
EcoO109I	RGGNCCY	3 cut(s)	88, 440, 793
EcoRII	CCWGG	6 cut(s)	286, 349, 376, 508, 559, 571
EcoT14I	CCWWGG	4 cut(s)	57, 91, 796, 923
EcoT22I	ATGCAT	1 cut(s)	220
EcoT38I	GRGCYC	2 cut(s)	190, 1230
ErhI	CCWWGG	4 cut(s)	57, 91, 796, 923
Esp3I	CGTCTC	1 cut(s)	764
FaeI	CATG	5 cut(s)	61, 320, 397, 701, 1178
FalI	AAGNNNNNCTT	2 cut(s)	1163, 1195
FaqI	GGGAC	3 cut(s)	426, 1621, 1694
FatI	CATG	5 cut(s)	57, 316, 393, 697, 1174
FauNDI	CATATG	1 cut(s)	220
FblI	GTMKAC	2 cut(s)	21, 1214
Fnu4HI	GCNGC	5 cut(s)	41, 885, 1272, 1518, 1617
FokI	GGATG	2 cut(s)	661, 1526
FriOI	GRGCYC	2 cut(s)	190, 1230
Fsp4HI	GCNGC	5 cut(s)	41, 885, 1272, 1518, 1617
FspBI	CTAG	2 cut(s)	579, 990
GlaI	GCGC	2 cut(s)	39, 1390
GluI	GCNGC	5 cut(s)	41, 885, 1272, 1518, 1617
GsaI	CCCAGC	2 cut(s)	813, 1124
GsuI	CTGGAG	2 cut(s)	531, 1356
HaeII	RGCGCY	1 cut(s)	41
HaeIII	GGCC	2 cut(s)	571, 1286
HapII	CCGG	2 cut(s)	10, 590
HhaI	GCGC	2 cut(s)	40, 1391
Hin1II	CATG	5 cut(s)	61, 320, 397, 701, 1178
Hin6I	GCGC	2 cut(s)	38, 1389
HinP1I	GCGC	2 cut(s)	38, 1389
HincII	GTYRAC	1 cut(s)	943
HindII	GTYRAC	1 cut(s)	943
HinfI	GANTC	5 cut(s)	959, 1304, 1315, 1424, 1490
HpaII	CCGG	2 cut(s)	10, 590
HphI	GGTGA	9 cut(s)	152, 332, 392, 604, 632, 887, 1287, 1373, 1640
Hpy188I	TCNGA	7 cut(s)	129, 235, 357, 1161, 1397, 1449, 1602
Hpy188III	TCNNGA	1 cut(s)	555
Hpy99I	CGWCG	1 cut(s)	1553
HpyAV	CCTTC	5 cut(s)	687, 1238, 1261, 1543, 1570
HpyCH4III	ACNGT	2 cut(s)	767, 1111
HpyCH4V	TGCA	9 cut(s)	110, 218, 736, 789, 887, 1054, 1082, 1133, 1380
HpyF10VI	GCNNNNNNNGC	5 cut(s)	200, 1139, 1386, 1462, 1526
HpyF3I	CTNAG	5 cut(s)	204, 1043, 1229, 1479, 1718
Hsp92II	CATG	5 cut(s)	61, 320, 397, 701, 1178
HspAI	GCGC	2 cut(s)	38, 1389
Kzo9I	GATC	2 cut(s)	313, 1397
LguI	GCTCTTC	1 cut(s)	50
LmnI	GCTCC	6 cut(s)	100, 185, 296, 663, 740, 1225
Lsp1109I	GCAGC	3 cut(s)	27, 871, 1283
LweI	GCATC	6 cut(s)	1063, 1069, 1084, 1339, 1389, 1504
MabI	ACCWGGT	3 cut(s)	349, 376, 559
MaeI	CTAG	2 cut(s)	579, 990
MaeIII	GTNAC	1 cut(s)	380
MalI	GATC	2 cut(s)	315, 1399
MbiI	CCGCTC	1 cut(s)	103
MboI	GATC	2 cut(s)	313, 1397
MflI	RGATCY	1 cut(s)	1397
MhlI	GDGCHC	4 cut(s)	190, 1135, 1230, 1719
MluCI	AATT	6 cut(s)	123, 253, 983, 1218, 1342, 1428
MlyI	GAGTC	1 cut(s)	968
MmeI	TCCRAC	2 cut(s)	733, 1130
Mph1103I	ATGCAT	1 cut(s)	220
MroXI	GAANNNNTTC	4 cut(s)	11, 134, 636, 953
MseI	TTAA	6 cut(s)	53, 746, 1181, 1221, 1538, 1674
MslI	CAYNNNNRTG	1 cut(s)	29
MspI	CCGG	2 cut(s)	10, 590
MspR9I	CCNGG	6 cut(s)	288, 351, 378, 510, 561, 573
Mva1269I	GAATGC	3 cut(s)	112, 822, 1391
MvaI	CCWGG	6 cut(s)	288, 351, 378, 510, 561, 573
MvnI	CGCG	1 cut(s)	1616
MwoI	GCNNNNNNNGC	5 cut(s)	200, 1139, 1386, 1462, 1526
NcoI	CCATGG	1 cut(s)	57
NdeI	CATATG	1 cut(s)	220
NdeII	GATC	2 cut(s)	313, 1397
NlaIII	CATG	5 cut(s)	61, 320, 397, 701, 1178
NlaIV	GGNNCC	6 cut(s)	304, 412, 442, 659, 742, 777
NmeAIII	GCCGAG	1 cut(s)	547
NmuCI	GTSAC	1 cut(s)	380
NsiI	ATGCAT	1 cut(s)	220
NspI	RCATGY	2 cut(s)	701, 1178
NspV	TTCGAA	1 cut(s)	932
PciI	ACATGT	1 cut(s)	697
PciSI	GCTCTTC	1 cut(s)	50
PctI	GAATGC	3 cut(s)	112, 822, 1391
PdmI	GAANNNNTTC	4 cut(s)	11, 134, 636, 953
PfeI	GAWTC	4 cut(s)	1304, 1315, 1424, 1490
PflFI	GACNNNGTC	1 cut(s)	351
PfoI	TCCNGGA	1 cut(s)	286
PinAI	ACCGGT	1 cut(s)	9
PkrI	GCNGC	5 cut(s)	42, 886, 1273, 1519, 1618
PleI	GAGTC	1 cut(s)	967
PpsI	GAGTC	1 cut(s)	967
PpuMI	RGGWCCY	3 cut(s)	88, 440, 793
PscI	ACATGT	1 cut(s)	697
PshAI	GACNNNNGTC	1 cut(s)	1439
Psp124BI	GAGCTC	2 cut(s)	190, 1230
Psp5II	RGGWCCY	3 cut(s)	88, 440, 793
Psp6I	CCWGG	6 cut(s)	286, 349, 376, 508, 559, 571
PspFI	CCCAGC	2 cut(s)	809, 1120
PspGI	CCWGG	6 cut(s)	286, 349, 376, 508, 559, 571
PspN4I	GGNNCC	6 cut(s)	304, 412, 442, 659, 742, 777
PspPI	GGNCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
PspPPI	RGGWCCY	3 cut(s)	88, 440, 793
PstI	CTGCAG	1 cut(s)	738
PsuI	RGATCY	1 cut(s)	1397
PsyI	GACNNNGTC	1 cut(s)	351
RsaI	GTAC	2 cut(s)	836, 1323
RsaNI	GTAC	2 cut(s)	835, 1322
RseI	CAYNNNNRTG	1 cut(s)	29
SacI	GAGCTC	2 cut(s)	190, 1230
SapI	GCTCTTC	1 cut(s)	50
SaqAI	TTAA	6 cut(s)	53, 746, 1181, 1221, 1538, 1674
SatI	GCNGC	5 cut(s)	41, 885, 1272, 1518, 1617
Sau3AI	GATC	2 cut(s)	313, 1397
Sau96I	GGNCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
SbfI	CCTGCAGG	1 cut(s)	738
SchI	GAGTC	1 cut(s)	968
ScrFI	CCNGG	6 cut(s)	288, 351, 378, 510, 561, 573
SdaI	CCTGCAGG	1 cut(s)	738
SduI	GDGCHC	4 cut(s)	190, 1135, 1230, 1719
SexAI	ACCWGGT	3 cut(s)	349, 376, 559
SfaNI	GCATC	6 cut(s)	1063, 1069, 1084, 1339, 1389, 1504
SfcI	CTRYAG	4 cut(s)	72, 734, 1143, 1638
SfuI	TTCGAA	1 cut(s)	932
SinI	GGWCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
SmiMI	CAYNNNNRTG	1 cut(s)	29
SmlI	CTYRAG	1 cut(s)	189
SmoI	CTYRAG	1 cut(s)	189
Sse8387I	CCTGCAGG	1 cut(s)	738
Sse9I	AATT	6 cut(s)	123, 253, 983, 1218, 1342, 1428
SsiI	CCGC	6 cut(s)	103, 952, 1439, 1518, 1616, 1626
SspMI	CTAG	2 cut(s)	579, 990
SstI	GAGCTC	2 cut(s)	190, 1230
StyD4I	CCNGG	6 cut(s)	286, 349, 376, 508, 559, 571
StyI	CCWWGG	4 cut(s)	57, 91, 796, 923
TaaI	ACNGT	2 cut(s)	767, 1111
TaqI	TCGA	5 cut(s)	556, 932, 957, 1242, 1548
TasI	AATT	6 cut(s)	123, 253, 983, 1218, 1342, 1428
TatI	WGTACW	1 cut(s)	834
TauI	GCSGC	2 cut(s)	1520, 1619
TfiI	GAWTC	4 cut(s)	1304, 1315, 1424, 1490
Tru1I	TTAA	6 cut(s)	53, 746, 1181, 1221, 1538, 1674
Tru9I	TTAA	6 cut(s)	53, 746, 1181, 1221, 1538, 1674
TscAI	CASTG	5 cut(s)	451, 544, 1051, 1116, 1237
TseFI	GTSAC	1 cut(s)	380
TseI	GCWGC	3 cut(s)	40, 884, 1271
Tsp45I	GTSAC	1 cut(s)	380
TspDTI	ATGAA	5 cut(s)	252, 288, 447, 669, 1223
TspGWI	ACGGA	1 cut(s)	788
TspRI	CASTG	5 cut(s)	451, 544, 1051, 1116, 1237
Tth111I	GACNNNGTC	1 cut(s)	351
VneI	GTGCAC	1 cut(s)	1131
VpaK11BI	GGWCC	7 cut(s)	88, 347, 374, 440, 449, 793, 1087
XapI	RAATTY	1 cut(s)	123
XceI	RCATGY	2 cut(s)	701, 1178
XcmI	CCANNNNNNNNNTGG	1 cut(s)	368
XmiI	GTMKAC	2 cut(s)	21, 1214
XmnI	GAANNNNTTC	4 cut(s)	11, 134, 636, 953
XspI	CTAG	2 cut(s)	579, 990
Zsp2I	ATGCAT	1 cut(s)	220

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.