Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003105

Physical Location & Seq

Genomic Coordinates Reverse (-)

27373190 .. 27377224

Gene Structure 4035 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1191600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGAAGCCACATCTATTTTGTGGCACGGTAATTCTCTTCCTTTTCCTTTGCCGAACGATAGCTGACATCGATTCAGACAAGAAAGCCCTTCTCGATTTTATTTCTGCTGTTCCTCATGGCCGAAGAGTTAACTGGGATCCTGCTAATCCAGTCTGCAAGTCTTGGGTTGGCATCACTTGTACCTTGGATGGCACCCGTGTGGTTTCTGTCCGACTTCCAGGTGCTGGACTGTTTGGTCCTATACCAGCAAACACTCTTGGCAAGCTAGATGCCCTCATGATCCTTAGCCTTAGGTCTAACGGCCTCGGTGGGAATCTTCCATCTGATATTTTCTCACTTCCTTCCTTACGCTACATATTCCTTCAAAACAATAATCTTACGGGTAATATTCCCTCTTCTCTGAGTCCCAATCTTATCTTTCTTGATTTGTCGTCCAACTCCATCACCGGTAACATTCCAGCCACAATCCAAAACTTTACGCGTCTCATGGGGCTGAACCTCCAGAACAATTCCCTCACTGGATCTATTCCTGATATCAAAACTCCAAAGCTTATGCGTTTGAATTTGAGCTACAACCAACTTAATGGTTCGATTCCTCCTACCCTCCAAAAATTCCCCACTTCCTCCTTTGAAGGAAACTTGAAGTTATGTGGCTCACCACTAAACCATTGTTCTTCGATCACTCCCTCGCCTTCTCCATCCCCCAATCTTCCACCAACAGCCCCCTTGAAATCAAAAAATGGATCAAAGAGGAAGCTTAGTATAAAGGCTATCATCGCCATTGCAAGTGGTGGTTTTGCAGTGTTGTTTCTCTCAGTTCTGGTGATAGTGCTATGCTGTTTGAAGAAAAAAGACAGCGAAGGCAGTGCTGTAGGGAAAACAAAAGGCGAACAGCCAAAAGAGGACTTTGGAAGTGGGGTGCAAGAGGCAGAGAAGAACAAGCTGGTTTTCTTCGAAGGCTCTTCTTATAACTTTGATCTGGAGGATTTGCTAAAAGCTTCGGCTGAAGTCCTAGGAAAGGGGAGTTATGGGACTACTTATAAGGCCATCTTGGAGGAGGGAACGACAGTGGTGGTGAAAAGGATGAAAGAAGTGGTGGTCGGGAAAAGAGAGTTCGAACAGCAAATGGAGAATGCCGGGAGAATTAGTCAGCACTCAAACGTTGTTCCTCTTCGAGCTTTTTACTACTCCAAAGATGAGAAACTCCTGGTTTATGACTACATTCCAGCTGGTAGCTTCTCAGCATTATTGCACGGAAACAGGGAAAGCGGACAGCATCCACCAGACTGGGAAACCAGATTAAGGATCTCTCTTGGATGTGCCAAGGGCCTTGCCCACATCCATTCTGCCAGTGAAGGGAAATTCATCCATGGCAACGTCAAATCCTCCAATGTCCTCCTCATGCAGGACCTCACTGGCAGCATCTCAGACTTCGGCCTAGCTCCTCTCATGAACTTTACAGCTATCCCTTCCAGAAGCGTGGGCTACCGAGCTCCCGAGGTTATTGAGACCAAGAAGTCGTTTCAAAAATCCGATGTTTATAGTTTTGGTGTTATGCTCCTTGAGATGCTGACTGGCAAAGCACCAGTACAATCACCTGGACGCGACGACATTGTTGATCTTCCAAGATGGGTCCAGTCTGTTGTTCGAGAGGAATGGACAGCCGAGGTGTTTGACGTGGAGCTAATGAGGTATGAAAATATCGAAGAGGAGTTGGTACAAATGCTTCAAATAGCAATGACTTGCGTGGCAACGGTGCCAGACATGAGACCTACCATGGAGGAAGTTGTTAGGATGATCGAGGAAATCCGGCCACCGGATTCTGAGAACCGACCATCGTCAGAAGACAAGTCCAAGGACTCGAGTGTCCAGACCCCACAAGGGCCAGCCTCGCCGTACCAGCCGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

637

Amino Acids

69.55

Weight (kDa)

7.54

Isoelectric Point (pI)

43.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	24 - 61	3e-07	Leucine rich repeat N-terminal domain
PK_Tyr_Ser-Thr	PF07714	336 - 599	4.8e-34	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	336 - 598	1.3e-33	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08680 AT3G08680
fragaria_vesca	FvH4_6g09530 FvH4_6g09530 FvH4_6g09530 FvH4_6g09530 FvH4_6g09530 FvH4_6g09530
malus_domestica	MD12G1191600.v1.1
prunus_persica	Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1 Prupe.6G296300_v2.0.a1
pyrus_communis	pycom04g15600 pycom12g17870
rosa_chinensis	RchiOBHm_Chr3g0460121
rosa_laevigata	RLG00000025042
rosa_multiflora	Rmu_sc0000449.1_g000052
rosa_roxburghii	Rroxscaffold_6G00419820
rosa_rugosa	Rorug03G0039700 Rorug03G0039800 Rorug03G0039900 Rorug03G0040000 Rorug03G0040100
rosa_samantha	Rh3AG098100 Rh3BG101200 Rh3CG103300 Rh3DG102500
rosa_wichuraiana	Rw3G008280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	969, 1041
AasI	GACNNNNNNGTC	3 cut(s)	234, 1516, 1865
AccB1I	GGYRCC	2 cut(s)	191, 1756
AccB7I	CCANNNNNTGG	1 cut(s)	224
AccII	CGCG	2 cut(s)	481, 1605
AciI	CCGC	1 cut(s)	1269
AclI	AACGTT	1 cut(s)	1161
AclWI	GGATC	6 cut(s)	131, 144, 274, 529, 751, 1313
AcoI	YGGCCR	2 cut(s)	118, 1811
AcsI	RAATTY	3 cut(s)	562, 611, 1361
AcuI	CTGAAG	1 cut(s)	1026
AfaI	GTAC	4 cut(s)	181, 1590, 1719, 1898
AfiI	CCNNNNNNNGG	7 cut(s)	224, 1018, 1302, 1356, 1405, 1816, 1881
AflIII	ACRYGT	1 cut(s)	479
AgeI	ACCGGT	1 cut(s)	446
AgsI	TTSAA	8 cut(s)	365, 562, 632, 643, 730, 844, 1526, 1730
AjiI	CACGTC	1 cut(s)	1678
AjnI	CCWGG	3 cut(s)	217, 1206, 1597
AleI	CACNNNNGTG	1 cut(s)	197
Alw21I	GWGCWC	1 cut(s)	1495
Alw26I	GTCTC	3 cut(s)	488, 1502, 1762
AlwI	GGATC	6 cut(s)	131, 144, 274, 529, 751, 1313
AlwNI	CAGNNNCTG	1 cut(s)	224
Ama87I	CYCGRG	2 cut(s)	1496, 1861
AoxI	GGCC	7 cut(s)	118, 301, 1044, 1327, 1435, 1811, 1883
ApeKI	GCWGC	1 cut(s)	1419
ApoI	RAATTY	3 cut(s)	562, 611, 1361
AsiGI	ACCGGT	1 cut(s)	446
AspA2I	CCTAGG	1 cut(s)	1012
AspS9I	GGNCC	5 cut(s)	236, 1327, 1408, 1633, 1883
AsuC2I	CCSGG	1 cut(s)	1138
AsuHPI	GGTGA	5 cut(s)	436, 648, 835, 1087, 1587
AsuII	TTCGAA	2 cut(s)	954, 1116
AvaI	CYCGRG	2 cut(s)	1496, 1861
AvaII	GGWCC	3 cut(s)	236, 1408, 1633
AvrII	CCTAGG	1 cut(s)	1012
AxyI	CCTNAGG	1 cut(s)	290
BamHI	GGATCC	1 cut(s)	136
BanI	GGYRCC	2 cut(s)	191, 1756
BanII	GRGCYC	1 cut(s)	1495
BarI	GAAGNNNNNNTAC	2 cut(s)	1710, 1742
BbsI	GAAGAC	1 cut(s)	1851
Bbv12I	GWGCWC	1 cut(s)	1495
BbvI	GCAGC	1 cut(s)	1431
BccI	CCATC	7 cut(s)	182, 328, 449, 706, 1055, 1623, 1843
BceAI	ACGGC	2 cut(s)	316, 1879
BcgI	CGANNNNNNTGC	2 cut(s)	848, 882
BciT130I	CCWGG	3 cut(s)	219, 1208, 1599
BcnI	CCSGG	1 cut(s)	1138
BcoDI	GTCTC	3 cut(s)	488, 1502, 1762
BfaI	CTAG	3 cut(s)	266, 1013, 1439
BfmI	CTRYAG	1 cut(s)	870
BisI	GCNGC	1 cut(s)	1420
BlnI	CCTAGG	1 cut(s)	1012
BlsI	GCNGC	1 cut(s)	1421
Bme1390I	CCNGG	4 cut(s)	219, 1138, 1208, 1599
Bme18I	GGWCC	3 cut(s)	236, 1408, 1633
BmeT110I	CYCGRG	2 cut(s)	1496, 1861
BmgBI	CACGTC	1 cut(s)	1678
BmgT120I	GGNCC	5 cut(s)	236, 1327, 1408, 1633, 1883
BmiI	GGNNCC	4 cut(s)	138, 193, 1634, 1758
BmrFI	CCNGG	4 cut(s)	219, 1138, 1208, 1599
BmrI	ACTGGG	2 cut(s)	142, 1297
BmsI	GCATC	5 cut(s)	180, 259, 1285, 1431, 1557
BmuI	ACTGGG	2 cut(s)	142, 1297
BoxI	GACNNNNGTC	1 cut(s)	1837
BpiI	GAAGAC	1 cut(s)	1851
BpmI	CTGGAG	2 cut(s)	485, 1001
Bpu10I	CCTNAGC	1 cut(s)	284
Bpu14I	TTCGAA	2 cut(s)	954, 1116
BpuEI	CTTGAG	1 cut(s)	1583
BpuMI	CCSGG	1 cut(s)	1138
Bsa29I	ATCGAT	1 cut(s)	69
BsaI	GGTCTC	2 cut(s)	1502, 1762
BsaJI	CCNNGG	9 cut(s)	183, 304, 1012, 1323, 1369, 1497, 1665, 1776, 1854
BsaWI	WCCGGW	2 cut(s)	446, 1816
BsaXI	ACNNNNNCTCC	2 cut(s)	1132, 1162
Bsc4I	CCNNNNNNNGG	7 cut(s)	224, 1018, 1302, 1356, 1405, 1816, 1881
Bse118I	RCCGGY	1 cut(s)	446
Bse1I	ACTGG	9 cut(s)	137, 149, 523, 1292, 1350, 1420, 1579, 1586, 1636
Bse21I	CCTNAGG	1 cut(s)	290
Bse3DI	GCAATG	2 cut(s)	780, 1743
BseBI	CCWGG	3 cut(s)	219, 1208, 1599
BseCI	ATCGAT	1 cut(s)	69
BseDI	CCNNGG	9 cut(s)	183, 304, 1012, 1323, 1369, 1497, 1665, 1776, 1854
BseGI	GGATG	8 cut(s)	193, 698, 1089, 1276, 1322, 1338, 1365, 1800
BseLI	CCNNNNNNNGG	7 cut(s)	224, 1018, 1302, 1356, 1405, 1816, 1881
BseMI	GCAATG	2 cut(s)	780, 1743
BseMII	CTCAG	5 cut(s)	392, 828, 1254, 1440, 1815
BseNI	ACTGG	9 cut(s)	137, 149, 523, 1292, 1350, 1420, 1579, 1586, 1636
BseRI	GAGGAG	4 cut(s)	1070, 1388, 1434, 1724
BseXI	GCAGC	1 cut(s)	1431
Bsh1236I	CGCG	2 cut(s)	481, 1605
BshFI	GGCC	7 cut(s)	120, 303, 1046, 1329, 1437, 1813, 1885
BshNI	GGYRCC	2 cut(s)	191, 1756
BshTI	ACCGGT	1 cut(s)	446
BshVI	ATCGAT	1 cut(s)	69
BsiHKAI	GWGCWC	1 cut(s)	1495
BsiHKCI	CYCGRG	2 cut(s)	1496, 1861
BsiSI	CCGG	5 cut(s)	447, 1137, 1810, 1817, 1904
BslFI	GGGAC	2 cut(s)	390, 1045
BslI	CCNNNNNNNGG	7 cut(s)	224, 1018, 1302, 1356, 1405, 1816, 1881
BsmAI	GTCTC	3 cut(s)	488, 1502, 1762
BsmBI	CGTCTC	1 cut(s)	488
BsmFI	GGGAC	2 cut(s)	390, 1045
BsmI	GAATGC	1 cut(s)	1138
BsnI	GGCC	7 cut(s)	120, 303, 1046, 1329, 1437, 1813, 1885
Bso31I	GGTCTC	2 cut(s)	1502, 1762
BsoBI	CYCGRG	2 cut(s)	1496, 1861
Bsp119I	TTCGAA	2 cut(s)	954, 1116
Bsp1286I	GDGCHC	1 cut(s)	1495
Bsp143I	GATC	9 cut(s)	136, 279, 521, 678, 743, 976, 1305, 1618, 1797
Bsp19I	CCATGG	2 cut(s)	1369, 1776
BspACI	CCGC	1 cut(s)	1269
BspANI	GGCC	7 cut(s)	120, 303, 1046, 1329, 1437, 1813, 1885
BspCNI	CTCAG	5 cut(s)	393, 827, 1253, 1439, 1816
BspDI	ATCGAT	1 cut(s)	69
BspFNI	CGCG	2 cut(s)	481, 1605
BspHI	TCATGA	2 cut(s)	276, 1449
BspLI	GGNNCC	4 cut(s)	138, 193, 1634, 1758
BspPI	GGATC	6 cut(s)	131, 144, 274, 529, 751, 1313
BspQI	GCTCTTC	1 cut(s)	967
BspT104I	TTCGAA	2 cut(s)	954, 1116
BspT107I	GGYRCC	2 cut(s)	191, 1756
BspTNI	GGTCTC	2 cut(s)	1502, 1762
BsrDI	GCAATG	2 cut(s)	780, 1743
BsrFI	RCCGGY	1 cut(s)	446
BsrI	ACTGG	9 cut(s)	137, 149, 523, 1292, 1350, 1420, 1579, 1586, 1636
BssAI	RCCGGY	1 cut(s)	446
BssECI	CCNNGG	9 cut(s)	183, 304, 1012, 1323, 1369, 1497, 1665, 1776, 1854
BssMI	GATC	9 cut(s)	136, 279, 521, 678, 743, 976, 1305, 1618, 1797
BssT1I	CCWWGG	6 cut(s)	183, 1012, 1323, 1369, 1776, 1854
Bst2UI	CCWGG	3 cut(s)	219, 1208, 1599
Bst4CI	ACNGT	4 cut(s)	28, 231, 1069, 1756
Bst6I	CTCTTC	6 cut(s)	41, 118, 400, 967, 1176, 1701
BstBI	TTCGAA	2 cut(s)	954, 1116
BstC8I	GCNNGC	2 cut(s)	263, 1887
BstDEI	CTNAG	8 cut(s)	284, 290, 401, 758, 814, 1240, 1426, 1824
BstDSI	CCRYGG	2 cut(s)	1369, 1776
BstENI	CCTNNNNNAGG	2 cut(s)	1016, 1403
BstF5I	GGATG	8 cut(s)	193, 698, 1089, 1276, 1322, 1338, 1365, 1800
BstFNI	CGCG	2 cut(s)	481, 1605
BstKTI	GATC	9 cut(s)	139, 282, 524, 681, 746, 979, 1308, 1621, 1800
BstMAI	GTCTC	3 cut(s)	488, 1502, 1762
BstMBI	GATC	9 cut(s)	136, 279, 521, 678, 743, 976, 1305, 1618, 1797
BstMWI	GCNNNNNNNGC	3 cut(s)	776, 1891, 1900
BstNI	CCWGG	3 cut(s)	219, 1208, 1599
BstPAI	GACNNNNGTC	1 cut(s)	1837
BstSCI	CCNGG	4 cut(s)	217, 1136, 1206, 1597
BstSFI	CTRYAG	1 cut(s)	870
BstUI	CGCG	2 cut(s)	481, 1605
BstV1I	GCAGC	1 cut(s)	1431
BstV2I	GAAGAC	1 cut(s)	1851
BstX2I	RGATCY	3 cut(s)	136, 521, 1305
BstXI	CCANNNNNNTGG	3 cut(s)	584, 1287, 1480
BstYI	RGATCY	3 cut(s)	136, 521, 1305
Bsu15I	ATCGAT	1 cut(s)	69
Bsu36I	CCTNAGG	1 cut(s)	290
BsuRI	GGCC	7 cut(s)	120, 303, 1046, 1329, 1437, 1813, 1885
BsuTUI	ATCGAT	1 cut(s)	69
BtgI	CCRYGG	2 cut(s)	1369, 1776
BtgZI	GCGATG	1 cut(s)	760
BtrI	CACGTC	1 cut(s)	1678
BtsCI	GGATG	8 cut(s)	193, 698, 1089, 1276, 1322, 1338, 1365, 1800
BtsI	GCAGTG	2 cut(s)	807, 871
BtsIMutI	CAGTG	6 cut(s)	516, 807, 871, 1074, 1357, 1413
Cac8I	GCNNGC	2 cut(s)	263, 1887
CaiI	CAGNNNCTG	1 cut(s)	224
CciI	TCATGA	2 cut(s)	276, 1449
Cfr10I	RCCGGY	1 cut(s)	446
Cfr13I	GGNCC	5 cut(s)	236, 1327, 1408, 1633, 1883
ClaI	ATCGAT	1 cut(s)	69
CseI	GACGC	2 cut(s)	470, 1611
Csp6I	GTAC	4 cut(s)	180, 1589, 1718, 1897
CspAI	ACCGGT	1 cut(s)	446
CviAII	CATG	8 cut(s)	116, 277, 487, 1370, 1402, 1450, 1765, 1777
CviQI	GTAC	4 cut(s)	180, 1589, 1718, 1897
DdeI	CTNAG	8 cut(s)	284, 290, 401, 758, 814, 1240, 1426, 1824
DpnI	GATC	9 cut(s)	138, 281, 523, 680, 745, 978, 1307, 1620, 1799
DpnII	GATC	9 cut(s)	136, 279, 521, 678, 743, 976, 1305, 1618, 1797
DrdI	GACNNNNNNGTC	3 cut(s)	234, 1516, 1865
DseDI	GACNNNNNNGTC	3 cut(s)	234, 1516, 1865
EaeI	YGGCCR	2 cut(s)	118, 1811
Eam1104I	CTCTTC	6 cut(s)	41, 118, 400, 967, 1176, 1701
EarI	CTCTTC	6 cut(s)	41, 118, 400, 967, 1176, 1701
Ecl136II	GAGCTC	1 cut(s)	1493
Eco130I	CCWWGG	6 cut(s)	183, 1012, 1323, 1369, 1776, 1854
Eco24I	GRGCYC	1 cut(s)	1495
Eco31I	GGTCTC	2 cut(s)	1502, 1762
Eco32I	GATATC	1 cut(s)	535
Eco47I	GGWCC	3 cut(s)	236, 1408, 1633
Eco53kI	GAGCTC	1 cut(s)	1493
Eco57I	CTGAAG	1 cut(s)	1026
Eco81I	CCTNAGG	1 cut(s)	290
Eco88I	CYCGRG	2 cut(s)	1496, 1861
EcoICRI	GAGCTC	1 cut(s)	1493
EcoNI	CCTNNNNNAGG	2 cut(s)	1016, 1403
EcoO109I	RGGNCCY	2 cut(s)	1327, 1408
EcoRII	CCWGG	3 cut(s)	217, 1206, 1597
EcoRV	GATATC	1 cut(s)	535
EcoT14I	CCWWGG	6 cut(s)	183, 1012, 1323, 1369, 1776, 1854
EcoT38I	GRGCYC	1 cut(s)	1495
ErhI	CCWWGG	6 cut(s)	183, 1012, 1323, 1369, 1776, 1854
Esp3I	CGTCTC	1 cut(s)	488
FaeI	CATG	8 cut(s)	119, 280, 490, 1373, 1405, 1453, 1768, 1780
FalI	AAGNNNNNCTT	2 cut(s)	1034, 1066
FaqI	GGGAC	2 cut(s)	390, 1045
FatI	CATG	8 cut(s)	115, 276, 486, 1369, 1401, 1449, 1764, 1776
Fnu4HI	GCNGC	1 cut(s)	1420
FokI	GGATG	8 cut(s)	200, 685, 1096, 1263, 1325, 1329, 1352, 1807
FriOI	GRGCYC	1 cut(s)	1495
Fsp4HI	GCNGC	1 cut(s)	1420
FspBI	CTAG	3 cut(s)	266, 1013, 1439
GluI	GCNGC	1 cut(s)	1420
GsuI	CTGGAG	2 cut(s)	485, 1001
HaeIII	GGCC	7 cut(s)	120, 303, 1046, 1329, 1437, 1813, 1885
HapII	CCGG	5 cut(s)	447, 1137, 1810, 1817, 1904
HgaI	GACGC	2 cut(s)	470, 1611
Hin1II	CATG	8 cut(s)	119, 280, 490, 1373, 1405, 1453, 1768, 1780
HincII	GTYRAC	1 cut(s)	130
HindII	GTYRAC	1 cut(s)	130
HindIII	AAGCTT	3 cut(s)	548, 755, 996
HinfI	GANTC	6 cut(s)	71, 313, 403, 592, 1820, 1859
HpaI	GTTAAC	1 cut(s)	130
HpaII	CCGG	5 cut(s)	447, 1137, 1810, 1817, 1904
HphI	GGTGA	5 cut(s)	436, 648, 835, 1087, 1587
Hpy166II	GTNNAC	1 cut(s)	130
Hpy188I	TCNGA	8 cut(s)	76, 212, 325, 402, 1429, 1534, 1825, 1843
Hpy8I	GTNNAC	1 cut(s)	130
Hpy99I	CGWCG	1 cut(s)	1610
HpyAV	CCTTC	9 cut(s)	98, 351, 371, 626, 702, 854, 950, 1349, 1479
HpyCH4III	ACNGT	4 cut(s)	28, 231, 1069, 1756
HpyCH4IV	ACGT	3 cut(s)	1161, 1377, 1677
HpyCH4V	TGCA	6 cut(s)	156, 785, 800, 922, 1252, 1405
HpyF10VI	GCNNNNNNNGC	3 cut(s)	776, 1891, 1900
HpyF3I	CTNAG	8 cut(s)	284, 290, 401, 758, 814, 1240, 1426, 1824
HpySE526I	ACGT	3 cut(s)	1161, 1377, 1677
Hsp92II	CATG	8 cut(s)	119, 280, 490, 1373, 1405, 1453, 1768, 1780
KspAI	GTTAAC	1 cut(s)	130
Kzo9I	GATC	9 cut(s)	136, 279, 521, 678, 743, 976, 1305, 1618, 1797
LguI	GCTCTTC	1 cut(s)	967
LmnI	GCTCC	4 cut(s)	1447, 1498, 1563, 1681
Lsp1109I	GCAGC	1 cut(s)	1431
LweI	GCATC	5 cut(s)	180, 259, 1285, 1431, 1557
MaeI	CTAG	3 cut(s)	266, 1013, 1439
MaeII	ACGT	3 cut(s)	1161, 1377, 1677
MaeIII	GTNAC	1 cut(s)	449
MalI	GATC	9 cut(s)	138, 281, 523, 680, 745, 978, 1307, 1620, 1799
MboI	GATC	9 cut(s)	136, 279, 521, 678, 743, 976, 1305, 1618, 1797
MflI	RGATCY	3 cut(s)	136, 521, 1305
MhlI	GDGCHC	1 cut(s)	1495
MluCI	AATT	6 cut(s)	30, 508, 562, 611, 1143, 1361
MluI	ACGCGT	1 cut(s)	479
MlyI	GAGTC	2 cut(s)	412, 1853
MmeI	TCCRAC	2 cut(s)	235, 459
MseI	TTAA	3 cut(s)	129, 582, 1301
MslI	CAYNNNNRTG	1 cut(s)	197
MspA1I	CMGCKG	1 cut(s)	1229
MspI	CCGG	5 cut(s)	447, 1137, 1810, 1817, 1904
MspR9I	CCNGG	4 cut(s)	219, 1138, 1208, 1599
Mva1269I	GAATGC	1 cut(s)	1138
MvaI	CCWGG	3 cut(s)	219, 1208, 1599
MvnI	CGCG	2 cut(s)	481, 1605
MwoI	GCNNNNNNNGC	3 cut(s)	776, 1891, 1900
NciI	CCSGG	1 cut(s)	1138
NcoI	CCATGG	2 cut(s)	1369, 1776
NdeII	GATC	9 cut(s)	136, 279, 521, 678, 743, 976, 1305, 1618, 1797
NlaIII	CATG	8 cut(s)	119, 280, 490, 1373, 1405, 1453, 1768, 1780
NlaIV	GGNNCC	4 cut(s)	138, 193, 1634, 1758
NmeAIII	GCCGAG	1 cut(s)	1690
NspV	TTCGAA	2 cut(s)	954, 1116
OliI	CACNNNNGTG	1 cut(s)	197
PaeR7I	CTCGAG	1 cut(s)	1861
PagI	TCATGA	2 cut(s)	276, 1449
PciSI	GCTCTTC	1 cut(s)	967
PctI	GAATGC	1 cut(s)	1138
PfeI	GAWTC	4 cut(s)	71, 313, 592, 1820
PflMI	CCANNNNNTGG	1 cut(s)	224
PinAI	ACCGGT	1 cut(s)	446
PkrI	GCNGC	1 cut(s)	1421
PleI	GAGTC	2 cut(s)	411, 1853
PpsI	GAGTC	2 cut(s)	411, 1853
PpuMI	RGGWCCY	1 cut(s)	1408
PshAI	GACNNNNGTC	1 cut(s)	1837
PsiI	TTATAA	2 cut(s)	969, 1041
Psp124BI	GAGCTC	1 cut(s)	1495
Psp1406I	AACGTT	1 cut(s)	1161
Psp5II	RGGWCCY	1 cut(s)	1408
Psp6I	CCWGG	3 cut(s)	217, 1206, 1597
PspGI	CCWGG	3 cut(s)	217, 1206, 1597
PspN4I	GGNNCC	4 cut(s)	138, 193, 1634, 1758
PspPI	GGNCC	5 cut(s)	236, 1327, 1408, 1633, 1883
PspPPI	RGGWCCY	1 cut(s)	1408
PspXI	VCTCGAGB	1 cut(s)	1861
PstNI	CAGNNNCTG	1 cut(s)	224
PsuI	RGATCY	3 cut(s)	136, 521, 1305
PvuII	CAGCTG	1 cut(s)	1229
RsaI	GTAC	4 cut(s)	181, 1590, 1719, 1898
RsaNI	GTAC	4 cut(s)	180, 1589, 1718, 1897
RseI	CAYNNNNRTG	1 cut(s)	197
SacI	GAGCTC	1 cut(s)	1495
SapI	GCTCTTC	1 cut(s)	967
SaqAI	TTAA	3 cut(s)	129, 582, 1301
SatI	GCNGC	1 cut(s)	1420
Sau3AI	GATC	9 cut(s)	136, 279, 521, 678, 743, 976, 1305, 1618, 1797
Sau96I	GGNCC	5 cut(s)	236, 1327, 1408, 1633, 1883
SchI	GAGTC	2 cut(s)	412, 1853
ScrFI	CCNGG	4 cut(s)	219, 1138, 1208, 1599
SduI	GDGCHC	1 cut(s)	1495
SfaNI	GCATC	5 cut(s)	180, 259, 1285, 1431, 1557
SfcI	CTRYAG	1 cut(s)	870
Sfr274I	CTCGAG	1 cut(s)	1861
SfuI	TTCGAA	2 cut(s)	954, 1116
SinI	GGWCC	3 cut(s)	236, 1408, 1633
SlaI	CTCGAG	1 cut(s)	1861
SmiMI	CAYNNNNRTG	1 cut(s)	197
SmlI	CTYRAG	2 cut(s)	1562, 1861
SmoI	CTYRAG	2 cut(s)	1562, 1861
Sse9I	AATT	6 cut(s)	30, 508, 562, 611, 1143, 1361
SsiI	CCGC	1 cut(s)	1269
SspI	AATATT	1 cut(s)	388
SspMI	CTAG	3 cut(s)	266, 1013, 1439
SstI	GAGCTC	1 cut(s)	1495
StyD4I	CCNGG	4 cut(s)	217, 1136, 1206, 1597
StyI	CCWWGG	6 cut(s)	183, 1012, 1323, 1369, 1776, 1854
TaaI	ACNGT	4 cut(s)	28, 231, 1069, 1756
TaiI	ACGT	3 cut(s)	1164, 1380, 1680
TasI	AATT	6 cut(s)	30, 508, 562, 611, 1143, 1361
TatI	WGTACW	1 cut(s)	1588
TfiI	GAWTC	4 cut(s)	71, 313, 592, 1820
Tru1I	TTAA	3 cut(s)	129, 582, 1301
Tru9I	TTAA	3 cut(s)	129, 582, 1301
TscAI	CASTG	6 cut(s)	523, 807, 871, 1074, 1357, 1420
TseI	GCWGC	1 cut(s)	1419
TspDTI	ATGAA	5 cut(s)	17, 1100, 1354, 1466, 1710
TspGWI	ACGGA	1 cut(s)	1269
TspRI	CASTG	6 cut(s)	523, 807, 871, 1074, 1357, 1420
Van91I	CCANNNNNTGG	1 cut(s)	224
VpaK11BI	GGWCC	3 cut(s)	236, 1408, 1633
XagI	CCTNNNNNAGG	2 cut(s)	1016, 1403
XapI	RAATTY	3 cut(s)	562, 611, 1361
XhoI	CTCGAG	1 cut(s)	1861
XmaJI	CCTAGG	1 cut(s)	1012
XspI	CTAG	3 cut(s)	266, 1013, 1439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.