Rorug03G0040100
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003105
Physical Location & Seq
Forward (+)
3142762 .. 3143446
685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0040100.1

Sequence Viewer

Length: 555 bp
ATGGATGTCGTGAAGGGAAGGTGGACGAAGAATCCGATCATCAAGGCGTGGAAGCGATGCAGTACTTTACCTAAACAAAGCAGCACGAAAGGCTCTACAGCAGCTGCTTCATTGACAAGAAGCAAATCATGGAGCTCCAGTACTACTAGTAGTACTGGCACTTGCAAGCAGAAGAAGAACAAGGCCAAGGCATGCCAAGTCGCTCCGGCTGGGTGTTTCACAGTCTACGTTGGACCCGAAAAGCAGCGATTTGTGGTGAGGATGGAGTTTCTTAATCATCCATTGTTCAGGATGCTGTTGGAGGATGCAGCATTGGAGTATGGGTACAAAAGTGACGGTCCGATATTGCTTCCTTGCCATGTGAATCTGTTCTGCAATGTTGTGGCAGAGATGGAGAGCAATGATATCGATGAGGATAGGATCAGTGCTCCCAGTAATTGTTCTCCGATAAACTTCTGTCCGGCTCGTCGTAGAAACTGTGCCAGCAACAGAGGCTACGGTGGTGCTTATAGGGTACTTACTCCAACATCGTCATCATCAATGGTGGTTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

184

Amino Acids

20.2

Weight (kDa)

9.55

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 43 - 128 1.8e-24 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 225
AclWI GGATC 1 cut(s) 428
AfaI GTAC 5 cut(s) 64, 142, 154, 326, 516
AhlI ACTAGT 1 cut(s) 146
AluBI AGCT 2 cut(s) 104, 135
AluI AGCT 2 cut(s) 104, 135
Alw21I GWGCWC 2 cut(s) 137, 430
AlwI GGATC 1 cut(s) 428
AlwNI CAGNNNCTG 1 cut(s) 104
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 5 cut(s) 81, 101, 104, 244, 308
Asp700I GAANNNNTTC 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 233, 338
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 2 cut(s) 233, 338
BaeI ACNNNNGTAYC 2 cut(s) 316, 349
BanII GRGCYC 1 cut(s) 137
Bbv12I GWGCWC 2 cut(s) 137, 430
BbvI GCAGC 5 cut(s) 91, 93, 113, 256, 320
BccI CCATC 2 cut(s) 256, 385
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 2 cut(s) 147, 553
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 5 cut(s) 82, 102, 105, 245, 309
BlsI GCNGC 5 cut(s) 83, 103, 106, 246, 310
BmcAI AGTACT 3 cut(s) 64, 142, 154
Bme18I GGWCC 2 cut(s) 233, 338
BmgT120I GGNCC 2 cut(s) 233, 338
BmiI GGNNCC 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 3 cut(s) 47, 282, 295
BmuI ACTGGG 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 121
Bsa29I ATCGAT 1 cut(s) 408
BsaJI CCNNGG 1 cut(s) 186
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 308, 338
Bse1I ACTGG 3 cut(s) 138, 160, 432
Bse3DI GCAATG 2 cut(s) 382, 406
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 5 cut(s) 10, 267, 277, 297, 310
BseMI GCAATG 2 cut(s) 382, 406
BseNI ACTGG 3 cut(s) 138, 160, 432
BseXI GCAGC 5 cut(s) 91, 93, 113, 256, 320
BseYI CCCAGC 1 cut(s) 209
BshFI GGCC 1 cut(s) 185
BshVI ATCGAT 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 137, 430
BsiSI CCGG 2 cut(s) 206, 461
BsnI GGCC 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 137, 430
Bsp143I GATC 2 cut(s) 36, 420
BspANI GGCC 1 cut(s) 185
BspDI ATCGAT 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 235
BspPI GGATC 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 382, 406
BsrI ACTGG 3 cut(s) 138, 160, 432
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 2 cut(s) 36, 420
BssT1I CCWWGG 1 cut(s) 186
Bst4CI ACNGT 4 cut(s) 223, 338, 479, 500
BstC8I GCNNGC 3 cut(s) 167, 193, 484
BstEII GGTNACC 1 cut(s) 547
BstF5I GGATG 5 cut(s) 10, 267, 277, 297, 310
BstKTI GATC 2 cut(s) 39, 423
BstMBI GATC 2 cut(s) 36, 420
BstMWI GCNNNNNNNGC 2 cut(s) 90, 492
BstNSI RCATGY 1 cut(s) 195
BstPI GGTNACC 1 cut(s) 547
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 5 cut(s) 91, 93, 113, 256, 320
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 1 cut(s) 185
BsuTUI ATCGAT 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 70
BtsCI GGATG 5 cut(s) 10, 267, 277, 297, 310
BtsIMutI CAGTG 1 cut(s) 430
Cac8I GCNNGC 3 cut(s) 167, 193, 484
CaiI CAGNNNCTG 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 233, 338
ClaI ATCGAT 1 cut(s) 408
CpoI CGGWCCG 1 cut(s) 338
Csp6I GTAC 5 cut(s) 63, 141, 153, 325, 515
CspI CGGWCCG 1 cut(s) 338
CviAII CATG 3 cut(s) 129, 192, 359
CviJI RGCY 7 cut(s) 93, 104, 135, 185, 209, 464, 495
CviKI_1 RGCY 7 cut(s) 93, 104, 135, 185, 209, 464, 495
CviQI GTAC 5 cut(s) 63, 141, 153, 325, 515
DpnI GATC 2 cut(s) 38, 422
DpnII GATC 2 cut(s) 36, 420
Ecl136II GAGCTC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 137
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 2 cut(s) 233, 338
Eco53kI GAGCTC 1 cut(s) 135
Eco91I GGTNACC 1 cut(s) 547
EcoICRI GAGCTC 1 cut(s) 135
EcoO65I GGTNACC 1 cut(s) 547
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 132, 195, 362
FaiI YATR 5 cut(s) 130, 193, 321, 360, 510
FatI CATG 3 cut(s) 128, 191, 358
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 5 cut(s) 82, 102, 105, 245, 309
FokI GGATG 5 cut(s) 17, 264, 274, 304, 317
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 5 cut(s) 82, 102, 105, 245, 309
FspBI CTAG 2 cut(s) 147, 553
GluI GCNGC 5 cut(s) 82, 102, 105, 245, 309
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 121
HaeIII GGCC 1 cut(s) 185
HapII CCGG 2 cut(s) 206, 461
Hin1II CATG 3 cut(s) 132, 195, 362
HinfI GANTC 2 cut(s) 31, 364
HpaII CCGG 2 cut(s) 206, 461
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 2 cut(s) 24, 226
Hpy188I TCNGA 3 cut(s) 36, 342, 447
Hpy188III TCNNGA 2 cut(s) 10, 289
Hpy8I GTNNAC 2 cut(s) 24, 226
Hpy99I CGWCG 1 cut(s) 471
HpyAV CCTTC 2 cut(s) 7, 12
HpyCH4III ACNGT 4 cut(s) 223, 338, 479, 500
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 4 cut(s) 60, 165, 308, 375
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 492
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 3 cut(s) 132, 195, 362
Kzo9I GATC 2 cut(s) 36, 420
LmnI GCTCC 4 cut(s) 132, 140, 208, 433
LpnPI CCDG 8 cut(s) 141, 151, 195, 219, 274, 445, 474, 496
Lsp1109I GCAGC 5 cut(s) 91, 93, 113, 256, 320
LweI GCATC 3 cut(s) 47, 282, 295
MaeI CTAG 2 cut(s) 147, 553
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 332, 547
MalI GATC 2 cut(s) 38, 422
MboI GATC 2 cut(s) 36, 420
MboII GAAGA 3 cut(s) 40, 184, 187
MhlI GDGCHC 2 cut(s) 137, 430
MluCI AATT 1 cut(s) 436
MmeI TCCRAC 3 cut(s) 211, 279, 548
MnlI CCTC 4 cut(s) 252, 295, 406, 485
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 2 cut(s) 206, 461
MwoI GCNNNNNNNGC 2 cut(s) 90, 492
NdeII GATC 2 cut(s) 36, 420
NlaIII CATG 3 cut(s) 132, 195, 362
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 195
PaeI GCATGC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 234
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 2 cut(s) 31, 364
PkrI GCNGC 5 cut(s) 83, 103, 106, 246, 310
Psp124BI GAGCTC 1 cut(s) 137
PspEI GGTNACC 1 cut(s) 547
PspFI CCCAGC 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 2 cut(s) 233, 338
PstNI CAGNNNCTG 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 5 cut(s) 64, 142, 154, 326, 516
RsaNI GTAC 5 cut(s) 63, 141, 153, 325, 515
Rsr2I CGGWCCG 1 cut(s) 338
RsrII CGGWCCG 1 cut(s) 338
SacI GAGCTC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 5 cut(s) 82, 102, 105, 245, 309
Sau3AI GATC 2 cut(s) 36, 420
Sau96I GGNCC 2 cut(s) 233, 338
ScaI AGTACT 3 cut(s) 64, 142, 154
SduI GDGCHC 2 cut(s) 137, 430
SetI ASST 6 cut(s) 23, 73, 106, 137, 231, 554
SfaNI GCATC 3 cut(s) 47, 282, 295
SfcI CTRYAG 1 cut(s) 96
SinI GGWCC 2 cut(s) 233, 338
SpeI ACTAGT 1 cut(s) 146
SphI GCATGC 1 cut(s) 195
Sse9I AATT 1 cut(s) 436
SspMI CTAG 2 cut(s) 147, 553
SstI GAGCTC 1 cut(s) 137
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 223, 338, 479, 500
TaiI ACGT 1 cut(s) 231
TaqI TCGA 1 cut(s) 408
TasI AATT 1 cut(s) 436
TatI WGTACW 3 cut(s) 62, 140, 152
TfiI GAWTC 2 cut(s) 31, 364
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 5 cut(s) 81, 101, 104, 244, 308
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 99
TspRI CASTG 1 cut(s) 430
VpaK11BI GGWCC 2 cut(s) 233, 338
XceI RCATGY 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 2 cut(s) 147, 553
ZrmI AGTACT 3 cut(s) 64, 142, 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.