MD12G1194100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016361
Physical Location & Seq
Reverse (-)
27564251 .. 27566090
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1194100.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGAAAGCATGAACCGTGGAGGAAGTTTCTCCTTCAGCTGCTCCGAGTCTGACTTGAACTTTTCTTCCCTCTCACATTTCACCAACGACGACGACGAAGACGATGAATCTTACATCGAAATAGAACTTGACCATCACCATGATCACCCTAAACCATCAAGCCAAGAGGATGATGAGGAGGAGGATGAAGATCTTGGTCGTGGAGGACTAGACCGTGCGGATTATTTGCGCATATCGTTTTCGTCTAGTCTTCTCCAGGAGCTCTCCGCCACCAACCTCCCTCCCAATCACGACTCTGAGGCAGCTGATCACCCAGTAAACCAGATTCCGTCGTCGCCAACGGTGTCATATTCTTGCCCTCTGAGCTCTTCTTCCATGGAAGGCAGCTGTAGGAATCCGATAGGATTGGATGAGCCTTCAAGGCTCCGCAGAAGTACTAGCGCAAAAGGAAGATTACCCAGAGTCAACCGGCTAGTCAACACGCTGCTGTTTGGTCTCCGGTCATCATCAGAGTCGCCTACCCCCGCAGACGACGCTGACCTGGCACGTAGCAGGAAAGCATCAAACATCAAAACCTCAATAAACGGTGGCCTCATGAAGTTTCTCTTCAAGTTCCGAGCCATGAATCTTGGAGCACTGGTAGCTTCATTTGTAAAGCCCCGCCAAGTTGCTTGTGACGATGACGATCCTCATCAATCCCAAAACATTAATTGTCGATGCAAGAACAAGAAATCGAAGGAGAGTAGTAGCAGTACTCTTCAATGGTTGGTTCCAAAGAAGCAAGGGAAAAGCAGCAGGACCAAGAACTCTTCAGAAGCAGTAGGAGGAGGTGACAAGTCCAGAGTGTTATTGGAGACAAACTTGAGCGCAGTTAGAGGGGTTTTAGAAACAATAGGAACTAGCATGAGCACAGGCATTGGTCGCAAAGAAAGAAGAACAAGGAGCTGCCAAAGTTCAATTAAGTCTTCCCCAATTCACCAAGGATTTACAAGTGATGACCATAGTAATAAGGTATATCTTTATACAAGAGAAACCTCAATTCAGGCTGCAATTTCCCACTGCAAAACTTCATTTGAACAAACATCCATATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

365

Amino Acids

39.98

Weight (kDa)

6.15

Isoelectric Point (pI)

63.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016361)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g09310
malus_domestica MD12G1194100.v1.1
prunus_persica Prupe.6G298800_v2.0.a1
pyrus_communis pycom04g15810 pycom12g18030
rosa_chinensis RchiOBHm_Chr3g0459701
rosa_laevigata RLG00000025066
rosa_multiflora Rmu_sc0000240.1_g000023
rosa_roxburghii Rroxscaffold_6G00420070
rosa_rugosa Rorug03G0037500 Rorug03G0037600
rosa_samantha Rh3AG096100 Rh3BG098800 Rh3CG100700 Rh3DG100100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 230
AciI CCGC 5 cut(s) 218, 267, 427, 525, 661
AclWI GGATC 1 cut(s) 680
AcuI CTGAAG 2 cut(s) 19, 795
AfaI GTAC 2 cut(s) 436, 754
AgsI TTSAA 6 cut(s) 58, 420, 610, 761, 957, 1076
AjnI CCWGG 2 cut(s) 255, 540
AloI GAACNNNNNNTCC 2 cut(s) 50, 82
AluBI AGCT 7 cut(s) 39, 262, 305, 366, 387, 644, 945
AluI AGCT 7 cut(s) 39, 262, 305, 366, 387, 644, 945
Alw21I GWGCWC 4 cut(s) 264, 368, 637, 911
Alw26I GTCTC 2 cut(s) 500, 848
AlwI GGATC 1 cut(s) 680
AoxI GGCC 1 cut(s) 589
ApeKI GCWGC 7 cut(s) 39, 302, 384, 484, 792, 945, 1046
AseI ATTAAT 1 cut(s) 708
AspLEI GCGC 3 cut(s) 231, 443, 869
AspS9I GGNCC 1 cut(s) 798
AsuHPI GGTGA 6 cut(s) 73, 128, 137, 302, 842, 968
AvaII GGWCC 1 cut(s) 798
BanII GRGCYC 2 cut(s) 264, 368
BbsI GAAGAC 3 cut(s) 105, 242, 957
Bbv12I GWGCWC 4 cut(s) 264, 368, 637, 911
BbvI GCAGC 7 cut(s) 26, 314, 396, 471, 804, 932, 1033
BccI CCATC 2 cut(s) 141, 163
BciT130I CCWGG 2 cut(s) 257, 542
BclI TGATCA 2 cut(s) 142, 307
BcoDI GTCTC 2 cut(s) 500, 848
BfaI CTAG 5 cut(s) 209, 246, 438, 473, 900
BfmI CTRYAG 1 cut(s) 388
BglI GCCNNNNNGGC 1 cut(s) 421
BglII AGATCT 1 cut(s) 190
BisI GCNGC 7 cut(s) 40, 303, 385, 485, 793, 946, 1047
BlsI GCNGC 7 cut(s) 41, 304, 386, 486, 794, 947, 1048
BmcAI AGTACT 2 cut(s) 436, 754
Bme1390I CCNGG 2 cut(s) 257, 542
Bme18I GGWCC 1 cut(s) 798
BmgT120I GGNCC 1 cut(s) 798
BmiI GGNNCC 2 cut(s) 425, 771
BmrFI CCNGG 2 cut(s) 257, 542
BmrI ACTGGG 1 cut(s) 308
BmsI GCATC 2 cut(s) 569, 707
BmuI ACTGGG 1 cut(s) 308
BpiI GAAGAC 3 cut(s) 105, 242, 957
BpmI CTGGAG 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 883
BsaAI YACGTR 1 cut(s) 548
BsaBI GATNNNNATC 3 cut(s) 189, 684, 690
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 3 cut(s) 16, 375, 979
BsaWI WCCGGW 1 cut(s) 498
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bse118I RCCGGY 1 cut(s) 468
Bse1I ACTGG 2 cut(s) 314, 642
Bse8I GATNNNNATC 3 cut(s) 189, 684, 690
BseBI CCWGG 2 cut(s) 257, 542
BseDI CCNNGG 3 cut(s) 16, 375, 979
BseGI GGATG 4 cut(s) 175, 190, 415, 1082
BseJI GATNNNNATC 3 cut(s) 189, 684, 690
BseMII CTCAG 2 cut(s) 288, 353
BseNI ACTGG 2 cut(s) 314, 642
BseRI GAGGAG 3 cut(s) 191, 194, 840
BseXI GCAGC 7 cut(s) 26, 314, 396, 471, 804, 932, 1033
BshFI GGCC 1 cut(s) 591
BsiHKAI GWGCWC 4 cut(s) 264, 368, 637, 911
BsiSI CCGG 2 cut(s) 469, 499
BsmAI GTCTC 2 cut(s) 500, 848
BsnI GGCC 1 cut(s) 591
Bso31I GGTCTC 1 cut(s) 500
Bsp1286I GDGCHC 4 cut(s) 264, 368, 637, 911
Bsp143I GATC 4 cut(s) 142, 190, 307, 685
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 5 cut(s) 218, 267, 427, 525, 661
BspANI GGCC 1 cut(s) 591
BspCNI CTCAG 2 cut(s) 289, 354
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 2 cut(s) 425, 771
BspPI GGATC 1 cut(s) 680
BspQI GCTCTTC 1 cut(s) 373
BspTNI GGTCTC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 468
BsrI ACTGG 2 cut(s) 314, 642
BssAI RCCGGY 1 cut(s) 468
BssECI CCNNGG 3 cut(s) 16, 375, 979
BssMI GATC 4 cut(s) 142, 190, 307, 685
BssT1I CCWWGG 2 cut(s) 375, 979
Bst2UI CCWGG 2 cut(s) 257, 542
Bst4CI ACNGT 4 cut(s) 17, 215, 343, 587
Bst6I CTCTTC 4 cut(s) 373, 611, 762, 814
BstBAI YACGTR 1 cut(s) 548
BstDEI CTNAG 2 cut(s) 297, 362
BstDSI CCRYGG 2 cut(s) 16, 375
BstF5I GGATG 4 cut(s) 175, 190, 415, 1082
BstHHI GCGC 3 cut(s) 231, 443, 869
BstKTI GATC 4 cut(s) 145, 193, 310, 688
BstMAI GTCTC 2 cut(s) 500, 848
BstMBI GATC 4 cut(s) 142, 190, 307, 685
BstMWI GCNNNNNNNGC 6 cut(s) 363, 421, 533, 542, 641, 921
BstNI CCWGG 2 cut(s) 257, 542
BstSCI CCNGG 2 cut(s) 255, 540
BstSFI CTRYAG 1 cut(s) 388
BstV1I GCAGC 7 cut(s) 26, 314, 396, 471, 804, 932, 1033
BstV2I GAAGAC 3 cut(s) 105, 242, 957
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 1 cut(s) 591
BtgI CCRYGG 2 cut(s) 16, 375
BtsCI GGATG 4 cut(s) 175, 190, 415, 1082
BtsI GCAGTG 1 cut(s) 1057
BtsIMutI CAGTG 2 cut(s) 635, 1057
CciI TCATGA 1 cut(s) 594
CfoI GCGC 3 cut(s) 231, 443, 869
Cfr10I RCCGGY 1 cut(s) 468
Cfr13I GGNCC 1 cut(s) 798
CseI GACGC 1 cut(s) 542
Csp6I GTAC 2 cut(s) 435, 753
CviAII CATG 6 cut(s) 10, 140, 376, 595, 622, 904
CviQI GTAC 2 cut(s) 435, 753
DdeI CTNAG 2 cut(s) 297, 362
DpnI GATC 4 cut(s) 144, 192, 309, 687
DpnII GATC 4 cut(s) 142, 190, 307, 685
Eam1104I CTCTTC 4 cut(s) 373, 611, 762, 814
EarI CTCTTC 4 cut(s) 373, 611, 762, 814
EciI GGCGGA 1 cut(s) 256
Ecl136II GAGCTC 2 cut(s) 262, 366
Eco130I CCWWGG 2 cut(s) 375, 979
Eco24I GRGCYC 2 cut(s) 264, 368
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 798
Eco53kI GAGCTC 2 cut(s) 262, 366
Eco57I CTGAAG 2 cut(s) 19, 795
EcoICRI GAGCTC 2 cut(s) 262, 366
EcoRII CCWGG 2 cut(s) 255, 540
EcoT14I CCWWGG 2 cut(s) 375, 979
EcoT38I GRGCYC 2 cut(s) 264, 368
ErhI CCWWGG 2 cut(s) 375, 979
FaeI CATG 6 cut(s) 13, 143, 379, 598, 625, 907
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FatI CATG 6 cut(s) 9, 139, 375, 594, 621, 903
FauI CCCGC 2 cut(s) 532, 668
FbaI TGATCA 2 cut(s) 142, 307
Fnu4HI GCNGC 7 cut(s) 40, 303, 385, 485, 793, 946, 1047
FokI GGATG 4 cut(s) 182, 197, 422, 1069
FriOI GRGCYC 2 cut(s) 264, 368
Fsp4HI GCNGC 7 cut(s) 40, 303, 385, 485, 793, 946, 1047
FspBI CTAG 5 cut(s) 209, 246, 438, 473, 900
FspI TGCGCA 1 cut(s) 230
GlaI GCGC 3 cut(s) 230, 442, 868
GluI GCNGC 7 cut(s) 40, 303, 385, 485, 793, 946, 1047
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 1 cut(s) 591
HapII CCGG 2 cut(s) 469, 499
HgaI GACGC 1 cut(s) 542
HhaI GCGC 3 cut(s) 231, 443, 869
Hin1II CATG 6 cut(s) 13, 143, 379, 598, 625, 907
Hin6I GCGC 3 cut(s) 229, 441, 867
HinP1I GCGC 3 cut(s) 229, 441, 867
HincII GTYRAC 2 cut(s) 466, 478
HindII GTYRAC 2 cut(s) 466, 478
HinfI GANTC 8 cut(s) 47, 107, 293, 325, 394, 462, 512, 625
HpaII CCGG 2 cut(s) 469, 499
HphI GGTGA 6 cut(s) 73, 128, 137, 302, 842, 968
Hpy166II GTNNAC 3 cut(s) 319, 466, 478
Hpy188I TCNGA 8 cut(s) 46, 52, 298, 363, 399, 511, 617, 814
Hpy188III TCNNGA 4 cut(s) 290, 595, 840, 1092
Hpy8I GTNNAC 3 cut(s) 319, 466, 478
Hpy99I CGWCG 6 cut(s) 92, 95, 98, 334, 337, 536
HpyAV CCTTC 4 cut(s) 43, 374, 426, 730
HpyCH4III ACNGT 4 cut(s) 17, 215, 343, 587
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 3 cut(s) 720, 1049, 1062
HpyF10VI GCNNNNNNNGC 6 cut(s) 363, 421, 533, 542, 641, 921
HpyF3I CTNAG 2 cut(s) 297, 362
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 6 cut(s) 13, 143, 379, 598, 625, 907
HspAI GCGC 3 cut(s) 229, 441, 867
Ksp22I TGATCA 2 cut(s) 142, 307
Kzo9I GATC 4 cut(s) 142, 190, 307, 685
LguI GCTCTTC 1 cut(s) 373
LmnI GCTCC 5 cut(s) 47, 259, 429, 632, 942
Lsp1109I GCAGC 7 cut(s) 26, 314, 396, 471, 804, 932, 1033
LweI GCATC 2 cut(s) 569, 707
MaeI CTAG 5 cut(s) 209, 246, 438, 473, 900
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 2 cut(s) 674, 830
MalI GATC 4 cut(s) 144, 192, 309, 687
MboI GATC 4 cut(s) 142, 190, 307, 685
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 4 cut(s) 264, 368, 637, 911
MluCI AATT 5 cut(s) 709, 957, 972, 1038, 1050
MlyI GAGTC 4 cut(s) 56, 287, 471, 521
MseI TTAA 2 cut(s) 708, 960
MslI CAYNNNNRTG 1 cut(s) 138
MspA1I CMGCKG 3 cut(s) 39, 305, 387
MspI CCGG 2 cut(s) 469, 499
MspR9I CCNGG 2 cut(s) 257, 542
MvaI CCWGG 2 cut(s) 257, 542
MwoI GCNNNNNNNGC 6 cut(s) 363, 421, 533, 542, 641, 921
NcoI CCATGG 1 cut(s) 375
NdeII GATC 4 cut(s) 142, 190, 307, 685
NlaIII CATG 6 cut(s) 13, 143, 379, 598, 625, 907
NlaIV GGNNCC 2 cut(s) 425, 771
NmuCI GTSAC 2 cut(s) 674, 830
NsbI TGCGCA 1 cut(s) 230
PagI TCATGA 1 cut(s) 594
PciSI GCTCTTC 1 cut(s) 373
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 99
PfeI GAWTC 4 cut(s) 107, 325, 394, 625
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 7 cut(s) 41, 304, 386, 486, 794, 947, 1048
PleI GAGTC 4 cut(s) 55, 287, 470, 520
PpsI GAGTC 4 cut(s) 55, 287, 470, 520
Ppu21I YACGTR 1 cut(s) 548
PshBI ATTAAT 1 cut(s) 708
Psp124BI GAGCTC 2 cut(s) 264, 368
Psp6I CCWGG 2 cut(s) 255, 540
PspGI CCWGG 2 cut(s) 255, 540
PspN4I GGNNCC 2 cut(s) 425, 771
PspPI GGNCC 1 cut(s) 798
PsuI RGATCY 1 cut(s) 190
PvuII CAGCTG 3 cut(s) 39, 305, 387
RsaI GTAC 2 cut(s) 436, 754
RsaNI GTAC 2 cut(s) 435, 753
RseI CAYNNNNRTG 1 cut(s) 138
SacI GAGCTC 2 cut(s) 264, 368
SapI GCTCTTC 1 cut(s) 373
SaqAI TTAA 2 cut(s) 708, 960
SatI GCNGC 7 cut(s) 40, 303, 385, 485, 793, 946, 1047
Sau3AI GATC 4 cut(s) 142, 190, 307, 685
Sau96I GGNCC 1 cut(s) 798
ScaI AGTACT 2 cut(s) 436, 754
SchI GAGTC 4 cut(s) 56, 287, 471, 521
ScrFI CCNGG 2 cut(s) 257, 542
SduI GDGCHC 4 cut(s) 264, 368, 637, 911
SfaNI GCATC 2 cut(s) 569, 707
SfcI CTRYAG 1 cut(s) 388
SinI GGWCC 1 cut(s) 798
SmiMI CAYNNNNRTG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 862
SmoI CTYRAG 1 cut(s) 862
Sse9I AATT 5 cut(s) 709, 957, 972, 1038, 1050
SsiI CCGC 5 cut(s) 218, 267, 427, 525, 661
SspMI CTAG 5 cut(s) 209, 246, 438, 473, 900
SstI GAGCTC 2 cut(s) 264, 368
StyD4I CCNGG 2 cut(s) 255, 540
StyI CCWWGG 2 cut(s) 375, 979
TaaI ACNGT 4 cut(s) 17, 215, 343, 587
TaiI ACGT 1 cut(s) 550
TaqI TCGA 3 cut(s) 117, 715, 734
TasI AATT 5 cut(s) 709, 957, 972, 1038, 1050
TatI WGTACW 2 cut(s) 434, 752
TfiI GAWTC 4 cut(s) 107, 325, 394, 625
Tru1I TTAA 2 cut(s) 708, 960
Tru9I TTAA 2 cut(s) 708, 960
TscAI CASTG 2 cut(s) 642, 1064
TseFI GTSAC 2 cut(s) 674, 830
TseI GCWGC 7 cut(s) 39, 302, 384, 484, 792, 945, 1046
Tsp45I GTSAC 2 cut(s) 674, 830
TspDTI ATGAA 7 cut(s) 26, 120, 201, 611, 636, 638, 1059
TspGWI ACGGA 1 cut(s) 318
TspRI CASTG 2 cut(s) 642, 1064
VpaK11BI GGWCC 1 cut(s) 798
VspI ATTAAT 1 cut(s) 708
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XspI CTAG 5 cut(s) 209, 246, 438, 473, 900
ZrmI AGTACT 2 cut(s) 436, 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.