RLG00000025066

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016361
Physical Location & Seq
Reverse (-)
41734839 .. 41736532
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025066

Sequence Viewer

Length: 1170 bp
ATGGAAAGAATGAGTTGTAGTACTCTGCGAGATCTGGGCTGTGGAGGAAGCTTCTCCTTCAGCTGCTCCGACTCCGACTTAAACTTCTCCTCCGCCCTCTCCCATTTCACTGACGACGTCGGTGATGATGTTGTTGAAGAAGACGATGATAGTGACGTCGACGATGAAGAATCATACATCGAAATAGCACTGGATCAACACCATCCTAAAGCAAGCCATGATGATGGTGATGGAGACCGGTTAGACCAGGTCAATTACCTGCGCATATCGTTTTCGTCAAGCTTTCCCTTCCCAGACTTCTCCAACCACACCACCAACCCTAATACTCAAGACGACACGAACATCGTCTCCGAGACGACTACTGACCCAGCTGGAGCAGAAAAGCAGGCAGCATCATCTTCATCAATGACGTCAATATCAGAGTCGTCCACCTCAATGGAAGACTTTAGAGGCTCTGATCATGATGTTAGAACCAAAAGGCCTAAATTGCCGGCAGTCAACCGCCTAGTCAATACGTTGTTCTTTAGTCTTTGGTCATCGTCGGACACTACCGCCAAGAAACTGAGCACCGCTCAGGACCATTATAATATTAGTCATCCCCAATTGCACGTGGACAACGATGACTTCGTACAGAACAGGAAAGTATCAAACATAAAAACCACAGTCAATGGCGGCATCATGAAGTTGCTTTTCAAGTTTCGAGCTATGAATCTTGGAACTTTGGTAGCTTCATTTGTGAAACCCCGAAAAGTTGTTTGTCCTCCTGATCAAGAGTACTGCTATTTTAGTAACGGAGTACGCAAGAACAAGAAATCAAAAGCTCAGTCGACTCTTCAGAATCAGAAGCCCTTAGACAAATTCTTTGTACAAAAGAAGCAGCAGGGAAAGCGTGTCAAGATGGACTCTTCACGAGAGGTAGGAGGCGGTGAGAAATCAAGAGTGTTGGAGAAAAAGTTGAACGCAGTTAGAGGGGTTTTAGAAGCCATTGGAAGTAGTATGAGCACAGGCGTTAACCGCAAAGAAACAAGAACAAGGAGCTGCCCAAGTTCTATCAAGTCCTCTCCAATTCATAAAGAATTTGCAGGTGATCAGAGTAAAGTATTTGCAAGAGAAACCTCAATTCAGGCTGCAATTGCCCATTGTAAAATTTCATTTGAACAAATATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

390

Amino Acids

43.09

Weight (kDa)

6.28

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016361)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g09310
malus_domestica MD12G1194100.v1.1
prunus_persica Prupe.6G298800_v2.0.a1
pyrus_communis pycom04g15810 pycom12g18030
rosa_chinensis RchiOBHm_Chr3g0459701
rosa_laevigata RLG00000025066
rosa_multiflora Rmu_sc0000240.1_g000023
rosa_roxburghii Rroxscaffold_6G00420070
rosa_rugosa Rorug03G0037500 Rorug03G0037600
rosa_samantha Rh3AG096100 Rh3BG098800 Rh3CG100700 Rh3DG100100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AarI CACCTGC 1 cut(s) 1073
AatII GACGTC 3 cut(s) 120, 159, 413
Acc16I TGCGCA 1 cut(s) 263
Acc36I ACCTGC 2 cut(s) 267, 1073
AccBSI CCGCTC 1 cut(s) 572
AccI GTMKAC 2 cut(s) 159, 827
AciI CCGC 7 cut(s) 93, 502, 552, 570, 672, 924, 1015
AclWI GGATC 1 cut(s) 201
AcsI RAATTY 3 cut(s) 857, 1076, 1146
AcuI CTGAAG 2 cut(s) 43, 818
AcvI CACGTG 1 cut(s) 610
AcyI GRCGYC 3 cut(s) 117, 156, 410
AfaI GTAC 5 cut(s) 22, 630, 776, 798, 867
AgeI ACCGGT 1 cut(s) 237
AgsI TTSAA 4 cut(s) 137, 694, 958, 1157
AjnI CCWGG 1 cut(s) 246
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 8 cut(s) 51, 63, 282, 371, 704, 728, 821, 1038
AluI AGCT 8 cut(s) 51, 63, 282, 371, 704, 728, 821, 1038
Alw21I GWGCWC 2 cut(s) 569, 1004
Alw26I GTCTC 3 cut(s) 228, 347, 352
AlwI GGATC 1 cut(s) 201
AoxI GGCC 1 cut(s) 479
ApeKI GCWGC 5 cut(s) 63, 389, 877, 1038, 1127
ApoI RAATTY 3 cut(s) 857, 1076, 1146
ArsI GACNNNNNNTTYG 4 cut(s) 809, 841, 845, 877
AsiGI ACCGGT 1 cut(s) 237
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 577
AsuHPI GGTGA 4 cut(s) 134, 239, 938, 1097
AvaII GGWCC 1 cut(s) 577
BauI CACGAG 1 cut(s) 909
BbrPI CACGTG 1 cut(s) 610
BbsI GAAGAC 2 cut(s) 147, 447
Bbv12I GWGCWC 2 cut(s) 569, 1004
BbvI GCAGC 5 cut(s) 50, 401, 889, 1025, 1114
BccI CCATC 4 cut(s) 210, 218, 224, 892
BciT130I CCWGG 1 cut(s) 248
BclI TGATCA 3 cut(s) 457, 766, 1087
BcoDI GTCTC 3 cut(s) 228, 347, 352
BfaI CTAG 1 cut(s) 506
BfuAI ACCTGC 2 cut(s) 267, 1073
BglII AGATCT 1 cut(s) 31
BisI GCNGC 6 cut(s) 64, 390, 673, 878, 1039, 1128
BlsI GCNGC 6 cut(s) 65, 391, 674, 879, 1040, 1129
BmcAI AGTACT 2 cut(s) 22, 776
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 577
BmgT120I GGNCC 1 cut(s) 577
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 2 cut(s) 401, 684
BpiI GAAGAC 2 cut(s) 147, 447
BplI GAGNNNNNCTC 2 cut(s) 556, 588
BpmI CTGGAG 1 cut(s) 393
Bpu10I CCTNAGC 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 610
BsaHI GRCGYC 3 cut(s) 117, 156, 410
BsaI GGTCTC 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 237
BsaXI ACNNNNNCTCC 6 cut(s) 74, 104, 332, 362, 938, 968
Bse118I RCCGGY 2 cut(s) 237, 490
Bse1I ACTGG 1 cut(s) 195
BseBI CCWGG 1 cut(s) 248
BseGI GGATG 2 cut(s) 202, 595
BseMII CTCAG 3 cut(s) 554, 587, 836
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 79
BseXI GCAGC 5 cut(s) 50, 401, 889, 1025, 1114
BseYI CCCAGC 1 cut(s) 367
BshFI GGCC 1 cut(s) 481
BshTI ACCGGT 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 569, 1004
BsiSI CCGG 2 cut(s) 238, 491
BsmAI GTCTC 3 cut(s) 228, 347, 352
BsmBI CGTCTC 2 cut(s) 347, 352
BsnI GGCC 1 cut(s) 481
Bso31I GGTCTC 1 cut(s) 228
Bsp1286I GDGCHC 2 cut(s) 569, 1004
Bsp1407I TGTACA 1 cut(s) 865
Bsp143I GATC 5 cut(s) 31, 193, 457, 766, 1087
BspACI CCGC 7 cut(s) 93, 502, 552, 570, 672, 924, 1015
BspANI GGCC 1 cut(s) 481
BspCNI CTCAG 3 cut(s) 555, 586, 835
BspHI TCATGA 2 cut(s) 460, 678
BspMI ACCTGC 2 cut(s) 267, 1073
BspPI GGATC 1 cut(s) 201
BspTNI GGTCTC 1 cut(s) 228
BsrBI CCGCTC 1 cut(s) 572
BsrFI RCCGGY 2 cut(s) 237, 490
BsrGI TGTACA 1 cut(s) 865
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 2 cut(s) 237, 490
BssMI GATC 5 cut(s) 31, 193, 457, 766, 1087
BssNI GRCGYC 3 cut(s) 117, 156, 410
BssSI CACGAG 1 cut(s) 909
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 1 cut(s) 664
Bst6I CTCTTC 2 cut(s) 837, 910
BstACI GRCGYC 3 cut(s) 117, 156, 410
BstAUI TGTACA 1 cut(s) 865
BstBAI YACGTR 1 cut(s) 610
BstC8I GCNNGC 3 cut(s) 214, 387, 492
BstDEI CTNAG 4 cut(s) 563, 573, 822, 850
BstF5I GGATG 2 cut(s) 202, 595
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 5 cut(s) 34, 196, 460, 769, 1090
BstMAI GTCTC 3 cut(s) 228, 347, 352
BstMBI GATC 5 cut(s) 31, 193, 457, 766, 1087
BstMWI GCNNNNNNNGC 4 cut(s) 487, 886, 1014, 1133
BstNI CCWGG 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 246
BstV1I GCAGC 5 cut(s) 50, 401, 889, 1025, 1114
BstV2I GAAGAC 2 cut(s) 147, 447
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 2 cut(s) 224, 436
BstYI RGATCY 1 cut(s) 31
BsuRI GGCC 1 cut(s) 481
BtsCI GGATG 2 cut(s) 202, 595
BtsIMutI CAGTG 2 cut(s) 108, 188
BveI ACCTGC 2 cut(s) 267, 1073
Cac8I GCNNGC 3 cut(s) 214, 387, 492
CciI TCATGA 2 cut(s) 460, 678
CfoI GCGC 1 cut(s) 264
Cfr10I RCCGGY 2 cut(s) 237, 490
Cfr13I GGNCC 1 cut(s) 577
CsiI ACCWGGT 1 cut(s) 246
Csp6I GTAC 5 cut(s) 21, 629, 775, 797, 866
CspAI ACCGGT 1 cut(s) 237
CviAII CATG 3 cut(s) 218, 461, 679
CviQI GTAC 5 cut(s) 21, 629, 775, 797, 866
DdeI CTNAG 4 cut(s) 563, 573, 822, 850
DpnI GATC 5 cut(s) 33, 195, 459, 768, 1089
DpnII GATC 5 cut(s) 31, 193, 457, 766, 1087
Eam1104I CTCTTC 2 cut(s) 837, 910
EarI CTCTTC 2 cut(s) 837, 910
EciI GGCGGA 1 cut(s) 82
Eco147I AGGCCT 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 577
Eco57I CTGAAG 2 cut(s) 43, 818
Eco72I CACGTG 1 cut(s) 610
EcoRII CCWGG 1 cut(s) 246
Esp3I CGTCTC 2 cut(s) 347, 352
FaeI CATG 3 cut(s) 221, 464, 682
FatI CATG 3 cut(s) 217, 460, 678
FbaI TGATCA 3 cut(s) 457, 766, 1087
FblI GTMKAC 2 cut(s) 159, 827
Fnu4HI GCNGC 6 cut(s) 64, 390, 673, 878, 1039, 1128
FokI GGATG 2 cut(s) 189, 582
Fsp4HI GCNGC 6 cut(s) 64, 390, 673, 878, 1039, 1128
FspBI CTAG 1 cut(s) 506
FspI TGCGCA 1 cut(s) 263
GlaI GCGC 1 cut(s) 263
GluI GCNGC 6 cut(s) 64, 390, 673, 878, 1039, 1128
GsaI CCCAGC 1 cut(s) 371
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 1 cut(s) 481
HapII CCGG 2 cut(s) 238, 491
HhaI GCGC 1 cut(s) 264
Hin1I GRCGYC 3 cut(s) 117, 156, 410
Hin1II CATG 3 cut(s) 221, 464, 682
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 4 cut(s) 160, 499, 828, 1012
HindII GTYRAC 4 cut(s) 160, 499, 828, 1012
HindIII AAGCTT 2 cut(s) 49, 280
HinfI GANTC 7 cut(s) 71, 170, 422, 709, 829, 838, 902
HpaI GTTAAC 1 cut(s) 1012
HpaII CCGG 2 cut(s) 238, 491
HphI GGTGA 4 cut(s) 134, 239, 938, 1097
Hpy166II GTNNAC 6 cut(s) 160, 429, 499, 613, 828, 1012
Hpy188I TCNGA 9 cut(s) 70, 76, 352, 421, 457, 544, 837, 843, 1092
Hpy8I GTNNAC 6 cut(s) 160, 429, 499, 613, 828, 1012
Hpy99I CGWCG 5 cut(s) 119, 122, 161, 164, 544
HpyAV CCTTC 2 cut(s) 67, 298
HpyCH4III ACNGT 1 cut(s) 664
HpyCH4IV ACGT 5 cut(s) 117, 156, 410, 515, 609
HpyCH4V TGCA 4 cut(s) 607, 1082, 1106, 1130
HpyF10VI GCNNNNNNNGC 4 cut(s) 487, 886, 1014, 1133
HpyF3I CTNAG 4 cut(s) 563, 573, 822, 850
HpySE526I ACGT 5 cut(s) 117, 156, 410, 515, 609
Hsp92I GRCGYC 3 cut(s) 117, 156, 410
Hsp92II CATG 3 cut(s) 221, 464, 682
HspAI GCGC 1 cut(s) 262
KroI GCCGGC 1 cut(s) 490
KroNI GCCGGC 1 cut(s) 492
Ksp22I TGATCA 3 cut(s) 457, 766, 1087
KspAI GTTAAC 1 cut(s) 1012
Kzo9I GATC 5 cut(s) 31, 193, 457, 766, 1087
LmnI GCTCC 3 cut(s) 71, 374, 1035
Lsp1109I GCAGC 5 cut(s) 50, 401, 889, 1025, 1114
LweI GCATC 2 cut(s) 401, 684
MabI ACCWGGT 1 cut(s) 246
MaeI CTAG 1 cut(s) 506
MaeII ACGT 5 cut(s) 117, 156, 410, 515, 609
MaeIII GTNAC 2 cut(s) 152, 788
MalI GATC 5 cut(s) 33, 195, 459, 768, 1089
MbiI CCGCTC 1 cut(s) 572
MboI GATC 5 cut(s) 31, 193, 457, 766, 1087
MboII GAAGA 7 cut(s) 149, 152, 179, 390, 452, 824, 897
MfeI CAATTG 2 cut(s) 602, 1131
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 569, 1004
MluCI AATT 9 cut(s) 253, 485, 602, 857, 1065, 1076, 1119, 1131, 1146
MlyI GAGTC 4 cut(s) 65, 431, 823, 896
MmeI TCCRAC 5 cut(s) 93, 99, 327, 522, 924
MroNI GCCGGC 1 cut(s) 490
MseI TTAA 2 cut(s) 80, 1011
MslI CAYNNNNRTG 2 cut(s) 222, 434
MspA1I CMGCKG 2 cut(s) 63, 371
MspI CCGG 2 cut(s) 238, 491
MspR9I CCNGG 1 cut(s) 248
MunI CAATTG 2 cut(s) 602, 1131
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 487, 886, 1014, 1133
NaeI GCCGGC 1 cut(s) 492
NdeII GATC 5 cut(s) 31, 193, 457, 766, 1087
NgoMIV GCCGGC 1 cut(s) 490
NlaIII CATG 3 cut(s) 221, 464, 682
NmuCI GTSAC 1 cut(s) 152
NsbI TGCGCA 1 cut(s) 263
PagI TCATGA 2 cut(s) 460, 678
PaqCI CACCTGC 1 cut(s) 1073
PceI AGGCCT 1 cut(s) 481
PcsI WCGNNNNNNNCGW 2 cut(s) 615, 624
PdiI GCCGGC 1 cut(s) 492
PfeI GAWTC 3 cut(s) 170, 709, 838
PflFI GACNNNGTC 2 cut(s) 116, 248
PinAI ACCGGT 1 cut(s) 237
PkrI GCNGC 6 cut(s) 65, 391, 674, 879, 1040, 1129
PleI GAGTC 4 cut(s) 65, 430, 823, 896
PmaCI CACGTG 1 cut(s) 610
PmlI CACGTG 1 cut(s) 610
PpsI GAGTC 4 cut(s) 65, 430, 823, 896
Ppu21I YACGTR 1 cut(s) 610
PsiI TTATAA 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 246
PspCI CACGTG 1 cut(s) 610
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 1 cut(s) 246
PspPI GGNCC 1 cut(s) 577
PsuI RGATCY 1 cut(s) 31
PsyI GACNNNGTC 2 cut(s) 116, 248
PvuII CAGCTG 2 cut(s) 63, 371
RsaI GTAC 5 cut(s) 22, 630, 776, 798, 867
RsaNI GTAC 5 cut(s) 21, 629, 775, 797, 866
RseI CAYNNNNRTG 2 cut(s) 222, 434
SalI GTCGAC 2 cut(s) 158, 826
SaqAI TTAA 2 cut(s) 80, 1011
SatI GCNGC 6 cut(s) 64, 390, 673, 878, 1039, 1128
Sau3AI GATC 5 cut(s) 31, 193, 457, 766, 1087
Sau96I GGNCC 1 cut(s) 577
ScaI AGTACT 2 cut(s) 22, 776
SchI GAGTC 4 cut(s) 65, 431, 823, 896
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 2 cut(s) 569, 1004
SexAI ACCWGGT 1 cut(s) 246
SfaNI GCATC 2 cut(s) 401, 684
SgrDI CGTCGACG 1 cut(s) 158
SinI GGWCC 1 cut(s) 577
SmiMI CAYNNNNRTG 2 cut(s) 222, 434
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 9 cut(s) 253, 485, 602, 857, 1065, 1076, 1119, 1131, 1146
SseBI AGGCCT 1 cut(s) 481
SsiI CCGC 7 cut(s) 93, 502, 552, 570, 672, 924, 1015
SspI AATATT 1 cut(s) 589
SspMI CTAG 1 cut(s) 506
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 664
TaiI ACGT 5 cut(s) 120, 159, 413, 518, 612
TaqI TCGA 4 cut(s) 159, 180, 700, 827
TasI AATT 9 cut(s) 253, 485, 602, 857, 1065, 1076, 1119, 1131, 1146
TatI WGTACW 3 cut(s) 20, 774, 865
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 3 cut(s) 170, 709, 838
Tru1I TTAA 2 cut(s) 80, 1011
Tru9I TTAA 2 cut(s) 80, 1011
TscAI CASTG 2 cut(s) 115, 195
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 5 cut(s) 63, 389, 877, 1038, 1127
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 7 cut(s) 180, 390, 695, 720, 722, 1058, 1140
TspGWI ACGGA 1 cut(s) 807
TspRI CASTG 2 cut(s) 115, 195
Tth111I GACNNNGTC 2 cut(s) 116, 248
VpaK11BI GGWCC 1 cut(s) 577
XapI RAATTY 3 cut(s) 857, 1076, 1146
XmiI GTMKAC 2 cut(s) 159, 827
XspI CTAG 1 cut(s) 506
ZraI GACGTC 3 cut(s) 118, 157, 411
ZrmI AGTACT 2 cut(s) 22, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.