MD12G1194400.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013238
Physical Location & Seq
Reverse (-)
27583460 .. 27584068
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1194400.v1.1.491

Sequence Viewer

Length: 609 bp
ATGATAGAAAATAGCAACCACAACAAACCCAACAAGCGAAAACGCTTATGTCTCATTACCATTGGTGTTATCCTGTTCGTGAACTTCCTACTCTTCCTCATCGCCCTGATTCTTGCCCTAACAGTTTTCAAGTCCAAACAGCCGAAAACTGAAATCCTCACCGCCTCCGTGGATGGCGTGGCTCCTCGCGTCACCTTCCCCGCTGTTCGGATCGAACTCAACATCACCGTCGACCTCACGATCAACGTCCAGAACCGAAACCACGCAAGCTTCAAGCACGGCGCGGGAAAGGCTTTGCTTCTTTACCGAGGCACACAGGTCGGGGATGTGGATTTGTACCCGGGTAACATTCCGGCGAGGGGCAACGCCACGCTGCCCTGCCGGCTTACTTTGCAGGTGGACCGGGTGGCGAACGACATAAGCAAATTGATCAGTGATGTGCTGGGGGGTGAGATTATGCTCCAAACGAAAACCCGGATTCCAGGTAGGGTTACGTTCTTGGGGTTCATCAAGAAGCATGCTGTCGCTGTATCGGCGTGCCAGTTGACGATTGGGTTTCCGAACATGAAGGTGAAAAAACAAGATTGCAAGACCAAGGCCAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

203

Amino Acids

22.21

Weight (kDa)

10.18

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 80 - 177 1.2e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013238)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05975
fragaria_vesca FvH4_6g09280
malus_domestica MD04G1179300.v1.1 MD12G1194400.v1.1
prunus_persica Prupe.6G299100_v2.0.a1
pyrus_communis pycom04g15840 pycom12g18060
rosa_chinensis RchiOBHm_Chr3g0459661
rosa_laevigata RLG00000025069
rosa_multiflora Rmu_sc0000240.1_g000019
rosa_roxburghii Rroxscaffold_6G00420100
rosa_rugosa Rorug03G0036900
rosa_samantha Rh3AG095800 Rh3BG098300 Rh3CG100300 Rh3DG099700
rosa_wichuraiana Rw0G013780 Rw0G017640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 385
Acc36I ACCTGC 1 cut(s) 385
AccI GTMKAC 1 cut(s) 231
AccII CGCG 2 cut(s) 189, 284
AciI CCGC 3 cut(s) 162, 201, 284
AclWI GGATC 1 cut(s) 218
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 2 cut(s) 207, 359
AgsI TTSAA 2 cut(s) 130, 274
AjnI CCWGG 1 cut(s) 481
AluBI AGCT 1 cut(s) 270
AluI AGCT 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 1 cut(s) 597
ApeKI GCWGC 1 cut(s) 373
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 1 cut(s) 400
AsuC2I CCSGG 4 cut(s) 341, 342, 404, 475
AsuHPI GGTGA 5 cut(s) 151, 184, 217, 461, 583
AvaI CYCGRG 1 cut(s) 340
AvaII GGWCC 1 cut(s) 400
BbvI GCAGC 1 cut(s) 360
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciT130I CCWGG 1 cut(s) 483
BclI TGATCA 1 cut(s) 429
BcnI CCSGG 4 cut(s) 341, 342, 404, 475
BcoDI GTCTC 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 385
BglI GCCNNNNNGGC 1 cut(s) 382
BisI GCNGC 1 cut(s) 374
BlsI GCNGC 1 cut(s) 375
Bme1390I CCNGG 5 cut(s) 341, 342, 404, 475, 483
Bme18I GGWCC 1 cut(s) 400
BmeT110I CYCGRG 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 400
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 5 cut(s) 341, 342, 404, 475, 483
BpuMI CCSGG 4 cut(s) 341, 342, 404, 475
BsaJI CCNNGG 4 cut(s) 168, 307, 340, 594
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 359
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 1 cut(s) 541
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 4 cut(s) 168, 307, 340, 594
BseGI GGATG 2 cut(s) 178, 331
BseLI CCNNNNNNNGG 2 cut(s) 207, 359
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 360
BseYI CCCAGC 1 cut(s) 442
Bsh1236I CGCG 2 cut(s) 189, 284
BshFI GGCC 1 cut(s) 599
BsiHKCI CYCGRG 1 cut(s) 340
BsiSI CCGG 5 cut(s) 341, 353, 382, 403, 475
BslI CCNNNNNNNGG 2 cut(s) 207, 359
BsmAI GTCTC 1 cut(s) 56
BsnI GGCC 1 cut(s) 599
BsoBI CYCGRG 1 cut(s) 340
Bsp143I GATC 3 cut(s) 210, 240, 429
BspACI CCGC 3 cut(s) 162, 201, 284
BspANI GGCC 1 cut(s) 599
BspFNI CGCG 2 cut(s) 189, 284
BspLI GGNNCC 1 cut(s) 183
BspMI ACCTGC 1 cut(s) 385
BspPI GGATC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 1 cut(s) 541
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 4 cut(s) 168, 307, 340, 594
BssMI GATC 3 cut(s) 210, 240, 429
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 124, 229
Bst6I CTCTTC 1 cut(s) 98
BstC8I GCNNGC 4 cut(s) 268, 383, 519, 538
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 2 cut(s) 178, 331
BstFNI CGCG 2 cut(s) 189, 284
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 3 cut(s) 213, 243, 432
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 3 cut(s) 210, 240, 429
BstMWI GCNNNNNNNGC 4 cut(s) 290, 382, 391, 533
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 1 cut(s) 521
BstSCI CCNGG 5 cut(s) 339, 340, 402, 473, 481
BstUI CGCG 2 cut(s) 189, 284
BstV1I GCAGC 1 cut(s) 360
BsuRI GGCC 1 cut(s) 599
BtgI CCRYGG 1 cut(s) 168
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 2 cut(s) 178, 331
BtsIMutI CAGTG 1 cut(s) 439
BveI ACCTGC 1 cut(s) 385
Cac8I GCNNGC 4 cut(s) 268, 383, 519, 538
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 400
Cfr9I CCCGGG 1 cut(s) 340
CseI GACGC 1 cut(s) 178
Csp6I GTAC 1 cut(s) 337
CviAII CATG 2 cut(s) 518, 565
CviJI RGCY 6 cut(s) 142, 182, 270, 293, 385, 599
CviKI_1 RGCY 6 cut(s) 142, 182, 270, 293, 385, 599
CviQI GTAC 1 cut(s) 337
DpnI GATC 3 cut(s) 212, 242, 431
DpnII GATC 3 cut(s) 210, 240, 429
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 594
Eco47I GGWCC 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 2 cut(s) 521, 568
FaiI YATR 5 cut(s) 49, 419, 458, 519, 566
FatI CATG 2 cut(s) 517, 564
FauI CCCGC 2 cut(s) 208, 277
FbaI TGATCA 1 cut(s) 429
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 1 cut(s) 374
FokI GGATG 2 cut(s) 185, 338
Fsp4HI GCNGC 1 cut(s) 374
GlaI GCGC 1 cut(s) 283
GluI GCNGC 1 cut(s) 374
GsaI CCCAGC 1 cut(s) 446
HaeIII GGCC 1 cut(s) 599
HapII CCGG 5 cut(s) 341, 353, 382, 403, 475
HgaI GACGC 1 cut(s) 178
HhaI GCGC 1 cut(s) 284
Hin1II CATG 2 cut(s) 521, 568
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 2 cut(s) 232, 546
HindII GTYRAC 2 cut(s) 232, 546
HindIII AAGCTT 1 cut(s) 268
HinfI GANTC 2 cut(s) 109, 478
HpaII CCGG 5 cut(s) 341, 353, 382, 403, 475
HphI GGTGA 5 cut(s) 151, 184, 217, 461, 583
Hpy166II GTNNAC 4 cut(s) 82, 232, 400, 546
Hpy188I TCNGA 2 cut(s) 210, 561
Hpy188III TCNNGA 4 cut(s) 79, 238, 250, 511
Hpy8I GTNNAC 4 cut(s) 82, 232, 400, 546
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 205, 562
HpyCH4III ACNGT 2 cut(s) 124, 229
HpyCH4IV ACGT 2 cut(s) 246, 494
HpyCH4V TGCA 2 cut(s) 394, 588
HpyF10VI GCNNNNNNNGC 4 cut(s) 290, 382, 391, 533
HpySE526I ACGT 2 cut(s) 246, 494
Hsp92II CATG 2 cut(s) 521, 568
HspAI GCGC 1 cut(s) 282
KroI GCCGGC 1 cut(s) 381
KroNI GCCGGC 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 3 cut(s) 210, 240, 429
LmnI GCTCC 2 cut(s) 187, 465
Lsp1109I GCAGC 1 cut(s) 360
MaeII ACGT 2 cut(s) 246, 494
MaeIII GTNAC 3 cut(s) 190, 344, 490
MalI GATC 3 cut(s) 212, 242, 431
MboI GATC 3 cut(s) 210, 240, 429
MboII GAAGA 1 cut(s) 85
MluCI AATT 1 cut(s) 425
MnlI CCTC 7 cut(s) 107, 167, 175, 195, 245, 302, 351
MroNI GCCGGC 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 5 cut(s) 341, 353, 382, 403, 475
MspR9I CCNGG 5 cut(s) 341, 342, 404, 475, 483
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 2 cut(s) 189, 284
MwoI GCNNNNNNNGC 4 cut(s) 290, 382, 391, 533
NaeI GCCGGC 1 cut(s) 383
NciI CCSGG 4 cut(s) 341, 342, 404, 475
NdeII GATC 3 cut(s) 210, 240, 429
NgoMIV GCCGGC 1 cut(s) 381
NlaIII CATG 2 cut(s) 521, 568
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 190
NspI RCATGY 1 cut(s) 521
PaeI GCATGC 1 cut(s) 521
PaqCI CACCTGC 1 cut(s) 385
PdiI GCCGGC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 109, 478
PkrI GCNGC 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 481
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 1 cut(s) 400
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 569
SalI GTCGAC 1 cut(s) 230
SatI GCNGC 1 cut(s) 374
Sau3AI GATC 3 cut(s) 210, 240, 429
Sau96I GGNCC 1 cut(s) 400
ScrFI CCNGG 5 cut(s) 341, 342, 404, 475, 483
SetI ASST 9 cut(s) 197, 237, 249, 272, 321, 399, 487, 497, 573
SinI GGWCC 1 cut(s) 400
SmaI CCCGGG 1 cut(s) 342
SmiMI CAYNNNNRTG 1 cut(s) 569
SphI GCATGC 1 cut(s) 521
Sse9I AATT 1 cut(s) 425
SsiI CCGC 3 cut(s) 162, 201, 284
StyD4I CCNGG 5 cut(s) 339, 340, 402, 473, 481
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 2 cut(s) 124, 229
TaiI ACGT 2 cut(s) 249, 497
TaqI TCGA 2 cut(s) 213, 231
TasI AATT 1 cut(s) 425
TfiI GAWTC 2 cut(s) 109, 478
TscAI CASTG 1 cut(s) 439
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 1 cut(s) 373
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 2 cut(s) 496, 581
TspGWI ACGGA 1 cut(s) 157
TspMI CCCGGG 1 cut(s) 340
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 400
XceI RCATGY 1 cut(s) 521
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmaI CCCGGG 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.