MD12G1198800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016360
Physical Location & Seq
Forward (+)
27977319 .. 27980853
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1198800.v1.1.491

Sequence Viewer

Length: 2460 bp
ATGGCTGCTGAAACTGTTGCCTCACTTCAAATTCCTACCACTGATGAGCAAGTTGAGAAGAAGATCATTGAAGGAGAAAAGGCTATCGAACAAGATGGTGTTTCTTTAACCAAAGAAAATGGAGAAGAAGCGCACAAAACTGAGGATTTAGAGGGTCTAGCAACACCATCCGAGCCTCCGGCAGTTGAGGCTGAGAAGGCTGCTGATGCTCCCGCGCAAGATGTTCCGCTTGTAGAAGAAACAAAATTGGAAAATGAAGCCGTTCCTGATTCAAAAACACTCGATATCCCAAAGCATGTGGTTGATGCTGTTGAGAGCCCGATAGAGGTTCAGAGTGCAATTGAGTCTGTAGAAAGAGAACTGCCCAAAGAAGTGGCCACAGATAGCATTGAAATGGGTCAGGTGGAGCAACCAAAGATTGTTGGTACTCCTGTGCCAGATGTTCCGCTCGTAGAAGAAACAAAGGCGGAAAATGAATCCATTCCTGATTCAGAAACACTCAATATTCCAAAGCAAGTGGCTGATGATGTTGAGATCCCGGCAGAGGTTCAAAGTGTAGTTGAGTCTGTAGTGAGAGAAGCACCGAAAGAACCATCCCTAGATAGCATTGAAGTAGGGAAAGTGGAGGAACCGAAGATAGTTGATGCTCCTCTATCATCAGTTGAAGCCATTGAGAAACCAGAGGAGCCATTGGAGGTCACTTCGGACGAAGGATCCATAGCACCAGTCCTAGAAAAGATTAAAGACTCAGAACCGGAGTTTTTTGAAGTGAAGGACAAACTAGAAGAACCTGAGCAAGTAGAGCCTGAAGAGATTGTGCAGGATGAGACAGTGAAAGATGAAGGCTCGATAGCTGTAAAAGCTGAGCCTACCAGAGTCTTGGAGGAAGGGAAAACTGAAACAAAGGACGCACCTAGTCTAGCTGTGGAGGAAAAACCAGAACAGCCCAAACTTGTGGAACAAGAAAAGAAACATGATGAAGTTGGGCTTGTGGAAAGTGTGGAGGTTAAAAAAGTGAAAGAGGAAGGATCTTCAGTTGATAAGGTTGAAAATTCAACAGTCGTGGAAGAAGAAAAAAATGATGAAGATAACAAGCCTAGTCGAATTGAAAATCTTGAGGAGGCTTCGCTTAGTCGAGAAGCTCAAATTGAAGGGGATGCTTCTCTACCTGATGTCACAGAAAAGTTAGCTACTGAGGACGAAAAGAAAGAAATAAGTGCAGTTGATGTGGTTGAGAAGCTAGCAGAAGAGCCAGCTGTGGAGACTGAAAAGGTCGGGGAAGAGAGTGTGGAAACCAAAGAGAATGAGAAGAGCAATATGATTCCTTTGGATTCAACTCAAATAATTAAAGAAGACGAAAATAAGGAATCAAGTGGGGTTGATGTGGTTGAGAAGCTAGAGGACGAGGCAGCTGTGGAACTGGAAAAGGTTGGAGAAAAGAAGGTGGAGACTGTAGCGGATGGAAAGAGAGATTTGGTAACTGAGGATTCAATTCAACCAATCATAGAGGAACAAAGGAAAGAATTAAATGGAGGTGATTTGATTGAGAAGCTAGTAGACGAGGATGTAGTGAAGTTGGAAAATTTTGGAGAAGAGAATGTGGCAAAGGATGTCAAAGCTGAAGATAATGAAGAGAGGAATGTGATAGCTGAGGATCCAGCTCTGCCAATTGAGGAGAGTAAAAAGAAAGAATTAGACAGCTCTGATGTGGTTGAGAAGCTAGCAGAGGAGGCGGTAGTGAAGGTAGAAAGTGGAGAACAAGAGATGGTGGCTAAGAATGGCGGAATTGAAGAGAACGAAAAGAGAAATGAAAAAGTTGAGGATTCAACTCAACCAATCGATGTGGTTGAGAAGGTAGCAGAAGAGGCAGTGGTGGAAACAGAAAAGCCTGCAGAAGAGAATGAAACAAAGAATGTGAAATTTGAAGACGACCAAAACAAAACTGTACTTGAAGAGGATGTCAAGAACTCTATTACTTCTCCCTCTGAATTTATAGAAAAATCATTTGAGGGAGCTGCAAAAGATGCTGAACCTGCGGTGGAAACTGAAGCAGCAGAAAAGATACAAAATGCGACTCCATCTGATGTTGAAACCAAGACAGATAAGAGTGCGGAGGAGAAGCCAGACGAAGTAAGTACAGCTGTTGAAGTTGATGAATCAGAAGTGAAAGGAGAGGAAACTGTTAAAACAGATGCTGATAGATTGGAGAAAATTAAGGACGAAGTTGCGAAACCTGACCAGAACTTAGAGCCTCCTCCCACCAAGGATGGTGATCAAGCAAAACCCCCTGAGGACCTGCCAAAGGAAGTTCCAGCTAAGTCCTCTCAGAAACAGTCGAATAACCTTCTGTCAAAGGTAAAACATTCACTGGTGAAGGCGAAGAAGGCTATCATAGGTGGGAAATCTCCAAGCTCGAAAAATCCTGCTATCGGAACAAAGGAGGATATCAAAGTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

820

Amino Acids

89.61

Weight (kDa)

4.3

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g08611
malus_domestica MD04G1185000.v1.1 MD12G1198800.v1.1
prunus_persica Prupe.6G303400_v2.0.a1
pyrus_communis pycom12g18520
rosa_chinensis RchiOBHm_Chr3g0458711
rosa_laevigata RLG00000025140
rosa_multiflora Rmu_sc0001634.1_g000013
rosa_roxburghii Rroxscaffold_6G00420850
rosa_rugosa Rorug03G0031400
rosa_samantha Rh3AG089000 Rh3BG091800 Rh3CG092200 Rh3DG092800
rosa_wichuraiana Rw3G007610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2041, 2304
AccBSI CCGCTC 1 cut(s) 448
AccI GTMKAC 1 cut(s) 1557
AccII CGCG 1 cut(s) 215
AciI CCGC 9 cut(s) 213, 227, 446, 467, 1457, 1733, 1782, 2036, 2111
AclWI GGATC 6 cut(s) 529, 708, 721, 1036, 1649, 1662
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 5 cut(s) 30, 1051, 1582, 1919, 1988
AcuI CTGAAG 4 cut(s) 828, 1017, 1641, 2067
AfaI GTAC 3 cut(s) 427, 1947, 2137
AfiI CCNNNNNNNGG 4 cut(s) 325, 544, 2302, 2429
Alw26I GTCTC 3 cut(s) 821, 1256, 1442
AlwI GGATC 6 cut(s) 529, 708, 721, 1036, 1649, 1662
AlwNI CAGNNNCTG 1 cut(s) 2195
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 5 cut(s) 5, 200, 1409, 2015, 2051
ApoI RAATTY 5 cut(s) 30, 1051, 1582, 1919, 1988
Asp700I GAANNNNTTC 2 cut(s) 261, 480
AspLEI GCGC 2 cut(s) 133, 217
AspS9I GGNCC 1 cut(s) 2293
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 3 cut(s) 1547, 2282, 2383
AsuNHI GCTAGC 2 cut(s) 1240, 1720
AvaII GGWCC 1 cut(s) 2293
AxyI CCTNAGG 1 cut(s) 2289
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BalI TGGCCA 1 cut(s) 377
BamHI GGATCC 2 cut(s) 713, 1654
BanII GRGCYC 1 cut(s) 320
BbsI GAAGAC 2 cut(s) 1359, 1932
BbvCI CCTCAGC 1 cut(s) 1650
BbvI GCAGC 4 cut(s) 187, 1421, 2002, 2063
BccI CCATC 7 cut(s) 89, 175, 601, 1454, 1759, 2086, 2261
BceAI ACGGC 1 cut(s) 245
BclI TGATCA 1 cut(s) 2272
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 3 cut(s) 821, 1256, 1442
BfmI CTRYAG 4 cut(s) 348, 567, 1452, 1890
BfuAI ACCTGC 2 cut(s) 2041, 2304
BisI GCNGC 5 cut(s) 6, 201, 1410, 2016, 2052
BlpI GCTNAGC 1 cut(s) 864
BlsI GCNGC 5 cut(s) 7, 202, 1411, 2017, 2053
Bme1390I CCNGG 1 cut(s) 539
Bme18I GGWCC 1 cut(s) 2293
BmgT120I GGNCC 1 cut(s) 2293
BmiI GGNNCC 4 cut(s) 630, 687, 715, 1656
BmrFI CCNGG 1 cut(s) 539
BmsI GCATC 6 cut(s) 196, 295, 634, 1147, 2014, 2182
BmtI GCTAGC 2 cut(s) 1244, 1724
BpiI GAAGAC 2 cut(s) 1359, 1932
Bpu10I CCTNAGC 2 cut(s) 792, 1650
Bpu1102I GCTNAGC 1 cut(s) 864
BpuEI CTTGAG 1 cut(s) 1136
BpuMI CCSGG 1 cut(s) 539
Bsa29I ATCGAT 1 cut(s) 1839
BsaBI GATNNNNATC 1 cut(s) 2271
BsaJI CCNNGG 1 cut(s) 2262
BsaWI WCCGGW 1 cut(s) 754
Bsc4I CCNNNNNNNGG 4 cut(s) 325, 544, 2302, 2429
Bse1I ACTGG 3 cut(s) 725, 1425, 2373
Bse21I CCTNAGG 1 cut(s) 2289
Bse8I GATNNNNATC 1 cut(s) 2271
BseCI ATCGAT 1 cut(s) 1839
BseDI CCNNGG 1 cut(s) 2262
BseGI GGATG 9 cut(s) 167, 593, 829, 1162, 1465, 1570, 1615, 1963, 2272
BseJI GATNNNNATC 1 cut(s) 2271
BseLI CCNNNNNNNGG 4 cut(s) 325, 544, 2302, 2429
BseNI ACTGG 3 cut(s) 725, 1425, 2373
BseRI GAGGAG 7 cut(s) 639, 698, 1133, 1688, 1742, 2129, 2244
BseXI GCAGC 4 cut(s) 187, 1421, 2002, 2063
BsgI GTGCAG 2 cut(s) 839, 1239
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 1839
BsiSI CCGG 3 cut(s) 179, 539, 755
BslI CCNNNNNNNGG 4 cut(s) 325, 544, 2302, 2429
BsmAI GTCTC 3 cut(s) 821, 1256, 1442
BsnI GGCC 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 6 cut(s) 63, 534, 713, 1028, 1654, 2272
Bsp1720I GCTNAGC 1 cut(s) 864
BspACI CCGC 9 cut(s) 213, 227, 446, 467, 1457, 1733, 1782, 2036, 2111
BspANI GGCC 1 cut(s) 377
BspDI ATCGAT 1 cut(s) 1839
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 4 cut(s) 630, 687, 715, 1656
BspMAI CTGCAG 1 cut(s) 1894
BspMI ACCTGC 2 cut(s) 2041, 2304
BspOI GCTAGC 2 cut(s) 1244, 1724
BspPI GGATC 6 cut(s) 529, 708, 721, 1036, 1649, 1662
BspQI GCTCTTC 2 cut(s) 1242, 1304
BsrBI CCGCTC 1 cut(s) 448
BsrI ACTGG 3 cut(s) 725, 1425, 2373
BssECI CCNNGG 1 cut(s) 2262
BssMI GATC 6 cut(s) 63, 534, 713, 1028, 1654, 2272
BssT1I CCWWGG 1 cut(s) 2262
Bst4CI ACNGT 7 cut(s) 16, 832, 1060, 1453, 1945, 2182, 2334
BstAPI GCANNNNNTGC 1 cut(s) 2024
BstC8I GCNNGC 4 cut(s) 1242, 1254, 1722, 1890
BstENI CCTNNNNNAGG 1 cut(s) 2300
BstF5I GGATG 9 cut(s) 167, 593, 829, 1162, 1465, 1570, 1615, 1963, 2272
BstFNI CGCG 1 cut(s) 215
BstHHI GCGC 2 cut(s) 133, 217
BstKTI GATC 6 cut(s) 66, 537, 716, 1031, 1657, 2275
BstMAI GTCTC 3 cut(s) 821, 1256, 1442
BstMBI GATC 6 cut(s) 63, 534, 713, 1028, 1654, 2272
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 537
BstSFI CTRYAG 4 cut(s) 348, 567, 1452, 1890
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 187, 1421, 2002, 2063
BstV2I GAAGAC 2 cut(s) 1359, 1932
BstX2I RGATCY 4 cut(s) 534, 713, 1028, 1654
BstXI CCANNNNNNTGG 3 cut(s) 373, 880, 955
BstYI RGATCY 4 cut(s) 534, 713, 1028, 1654
Bsu15I ATCGAT 1 cut(s) 1839
Bsu36I CCTNAGG 1 cut(s) 2289
BsuRI GGCC 1 cut(s) 377
BsuTUI ATCGAT 1 cut(s) 1839
BtsCI GGATG 9 cut(s) 167, 593, 829, 1162, 1465, 1570, 1615, 1963, 2272
BtsI GCAGTG 1 cut(s) 1875
BtsIMutI CAGTG 4 cut(s) 39, 837, 1875, 2366
BveI ACCTGC 2 cut(s) 2041, 2304
Cac8I GCNNGC 4 cut(s) 1242, 1254, 1722, 1890
CaiI CAGNNNCTG 1 cut(s) 2195
CfoI GCGC 2 cut(s) 133, 217
Cfr13I GGNCC 1 cut(s) 2293
ClaI ATCGAT 1 cut(s) 1839
CseI GACGC 1 cut(s) 917
Csp6I GTAC 3 cut(s) 426, 1946, 2136
CspCI CAANNNNNGTGG 8 cut(s) 279, 314, 498, 533, 1044, 1079, 1824, 1859
CviAII CATG 2 cut(s) 296, 974
CviQI GTAC 3 cut(s) 426, 1946, 2136
DpnI GATC 6 cut(s) 65, 536, 715, 1030, 1656, 2274
DpnII GATC 6 cut(s) 63, 534, 713, 1028, 1654, 2272
EaeI YGGCCR 1 cut(s) 375
EciI GGCGGA 2 cut(s) 482, 1797
Eco130I CCWWGG 1 cut(s) 2262
Eco24I GRGCYC 1 cut(s) 320
Eco32I GATATC 2 cut(s) 286, 2446
Eco47I GGWCC 1 cut(s) 2293
Eco57I CTGAAG 4 cut(s) 828, 1017, 1641, 2067
Eco81I CCTNAGG 1 cut(s) 2289
EcoNI CCTNNNNNAGG 1 cut(s) 2300
EcoO109I RGGNCCY 1 cut(s) 2293
EcoRV GATATC 2 cut(s) 286, 2446
EcoT14I CCWWGG 1 cut(s) 2262
EcoT38I GRGCYC 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 2262
FaeI CATG 2 cut(s) 299, 977
FaiI YATR 7 cut(s) 297, 719, 975, 1319, 1505, 1994, 2393
FalI AAGNNNNNCTT 2 cut(s) 972, 1004
FatI CATG 2 cut(s) 295, 973
FauI CCCGC 1 cut(s) 220
FbaI TGATCA 1 cut(s) 2272
FblI GTMKAC 1 cut(s) 1557
Fnu4HI GCNGC 5 cut(s) 6, 201, 1410, 2016, 2052
FokI GGATG 9 cut(s) 154, 580, 836, 1169, 1472, 1577, 1622, 1970, 2279
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 5 cut(s) 6, 201, 1410, 2016, 2052
GlaI GCGC 2 cut(s) 132, 216
GluI GCNGC 5 cut(s) 6, 201, 1410, 2016, 2052
HaeIII GGCC 1 cut(s) 377
HapII CCGG 3 cut(s) 179, 539, 755
HgaI GACGC 1 cut(s) 917
HhaI GCGC 2 cut(s) 133, 217
Hin1II CATG 2 cut(s) 299, 977
Hin6I GCGC 2 cut(s) 131, 215
HinP1I GCGC 2 cut(s) 131, 215
HpaII CCGG 3 cut(s) 179, 539, 755
HphI GGTGA 3 cut(s) 1547, 2282, 2383
Hpy166II GTNNAC 1 cut(s) 1558
Hpy188III TCNNGA 5 cut(s) 266, 485, 1115, 1136, 1963
Hpy8I GTNNAC 1 cut(s) 1558
HpyCH4III ACNGT 7 cut(s) 16, 832, 1060, 1453, 1945, 2182, 2334
HpyCH4V TGCA 5 cut(s) 338, 820, 1220, 1892, 2018
Hsp92II CATG 2 cut(s) 299, 977
HspAI GCGC 2 cut(s) 131, 215
Ksp22I TGATCA 1 cut(s) 2272
Kzo9I GATC 6 cut(s) 63, 534, 713, 1028, 1654, 2272
LguI GCTCTTC 2 cut(s) 1242, 1304
LmnI GCTCC 5 cut(s) 214, 406, 652, 685, 2012
Lsp1109I GCAGC 4 cut(s) 187, 1421, 2002, 2063
LweI GCATC 6 cut(s) 196, 295, 634, 1147, 2014, 2182
MaeIII GTNAC 3 cut(s) 697, 1174, 1477
MalI GATC 6 cut(s) 65, 536, 715, 1030, 1656, 2274
MbiI CCGCTC 1 cut(s) 448
MboI GATC 6 cut(s) 63, 534, 713, 1028, 1654, 2272
MfeI CAATTG 2 cut(s) 339, 1668
MflI RGATCY 4 cut(s) 534, 713, 1028, 1654
MhlI GDGCHC 1 cut(s) 320
MlsI TGGCCA 1 cut(s) 377
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 5 cut(s) 353, 572, 740, 885, 2068
MmeI TCCRAC 2 cut(s) 1411, 1557
Mox20I TGGCCA 1 cut(s) 377
MroXI GAANNNNTTC 2 cut(s) 261, 480
MscI TGGCCA 1 cut(s) 377
MseI TTAA 8 cut(s) 107, 741, 1008, 1347, 1526, 2184, 2214, 2454
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 3 cut(s) 1256, 1412, 2141
MspI CCGG 3 cut(s) 179, 539, 755
MspR9I CCNGG 1 cut(s) 539
MunI CAATTG 2 cut(s) 339, 1668
MvnI CGCG 1 cut(s) 215
NciI CCSGG 1 cut(s) 539
NdeII GATC 6 cut(s) 63, 534, 713, 1028, 1654, 2272
NheI GCTAGC 2 cut(s) 1240, 1720
NlaIII CATG 2 cut(s) 299, 977
NlaIV GGNNCC 4 cut(s) 630, 687, 715, 1656
NmuCI GTSAC 2 cut(s) 697, 1174
NspI RCATGY 1 cut(s) 299
PciSI GCTCTTC 2 cut(s) 1242, 1304
PdmI GAANNNNTTC 2 cut(s) 261, 480
PfeI GAWTC 9 cut(s) 269, 476, 488, 1321, 1331, 1367, 1487, 1823, 2156
PkrI GCNGC 5 cut(s) 7, 202, 1411, 2017, 2053
PleI GAGTC 5 cut(s) 352, 571, 740, 884, 2068
PpsI GAGTC 5 cut(s) 352, 571, 740, 884, 2068
PpuMI RGGWCCY 1 cut(s) 2293
Psp5II RGGWCCY 1 cut(s) 2293
PspN4I GGNNCC 4 cut(s) 630, 687, 715, 1656
PspPI GGNCC 1 cut(s) 2293
PspPPI RGGWCCY 1 cut(s) 2293
PstI CTGCAG 1 cut(s) 1894
PstNI CAGNNNCTG 1 cut(s) 2195
PsuI RGATCY 4 cut(s) 534, 713, 1028, 1654
PvuII CAGCTG 3 cut(s) 1256, 1412, 2141
RsaI GTAC 3 cut(s) 427, 1947, 2137
RsaNI GTAC 3 cut(s) 426, 1946, 2136
RseI CAYNNNNRTG 1 cut(s) 392
SapI GCTCTTC 2 cut(s) 1242, 1304
SaqAI TTAA 8 cut(s) 107, 741, 1008, 1347, 1526, 2184, 2214, 2454
SatI GCNGC 5 cut(s) 6, 201, 1410, 2016, 2052
Sau3AI GATC 6 cut(s) 63, 534, 713, 1028, 1654, 2272
Sau96I GGNCC 1 cut(s) 2293
SchI GAGTC 5 cut(s) 353, 572, 740, 885, 2068
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 6 cut(s) 196, 295, 634, 1147, 2014, 2182
SfcI CTRYAG 4 cut(s) 348, 567, 1452, 1890
SinI GGWCC 1 cut(s) 2293
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 1115
SmoI CTYRAG 1 cut(s) 1115
SsiI CCGC 9 cut(s) 213, 227, 446, 467, 1457, 1733, 1782, 2036, 2111
SspI AATATT 1 cut(s) 505
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 1 cut(s) 2262
TaaI ACNGT 7 cut(s) 16, 832, 1060, 1453, 1945, 2182, 2334
TaqI TCGA 8 cut(s) 87, 282, 848, 1102, 1135, 1839, 2336, 2414
TatI WGTACW 2 cut(s) 1945, 2135
TfiI GAWTC 9 cut(s) 269, 476, 488, 1321, 1331, 1367, 1487, 1823, 2156
Tru1I TTAA 8 cut(s) 107, 741, 1008, 1347, 1526, 2184, 2214, 2454
Tru9I TTAA 8 cut(s) 107, 741, 1008, 1347, 1526, 2184, 2214, 2454
TscAI CASTG 4 cut(s) 46, 837, 1875, 2373
TseFI GTSAC 2 cut(s) 697, 1174
TseI GCWGC 5 cut(s) 5, 200, 1409, 2015, 2051
Tsp45I GTSAC 2 cut(s) 697, 1174
TspDTI ATGAA 9 cut(s) 270, 489, 855, 993, 1098, 1644, 1824, 1917, 2169
TspRI CASTG 4 cut(s) 46, 837, 1875, 2373
VpaK11BI GGWCC 1 cut(s) 2293
XagI CCTNNNNNAGG 1 cut(s) 2300
XapI RAATTY 5 cut(s) 30, 1051, 1582, 1919, 1988
XceI RCATGY 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 1557
XmnI GAANNNNTTC 2 cut(s) 261, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.