MD12G1200400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008146
Physical Location & Seq
Reverse (-)
28117678 .. 28120454
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1200400.v1.1.491

Sequence Viewer

Length: 2013 bp
ATGCTCATAAAGCTCCAGTCTTTAACATTTTCCAAGAGATTCCCCTTCTGGGTCTGTTCGAATGCATGCTTTTCTGCACTTTCCTGCACTAAAACCATTGAAATGTTATCGGGAAACAATCAGGACCCAGAAAATTCATCCGACTTTGAACAAAATATCCAGTCTTTGAGGAATCAGCTTGTACCGGATAACTTAATCCGGGTTCTGGATAACACTGATGATTTGAGCTCAGCAGTGAAGGTGTCGAAATGGGCTTCCCTGCAAAAACGGTTTAATCACACCGCCGATACGTATTTTCGGATTGCTTTGAAACTGGGTTTGGCTGGAAATGTTGAGGAGATGGAGGGGTTTTGTCAAAATATGGTGAAAGATGGGTGTCCCGATGTCGAAGAGGCGCTTTTGGCACCGATTGATGTGTTTGTTAGGCATTGCAGATTAAGTGAGGCAATGAGGGTGCTTGTGAATTTGAAAGCAGGTGGCTTTAGGCCTTCAATTGAGACATTTAATGGTGTATTGGGTGCCCTTGTGAAGGATAAGAGAGATTTTCGAGATGTTTTGTTCGTCTATAAGGAGATTGTGACGGCAGGAATCATGCCAAGTATTGATACTTTGAATTATTTGTTAGAGGCATTGTTGGAGAGCGGCCGCATTGAGTCTGCTGTTGATCAGTATAGAAGAATGAAGAAGAAAGGGTGTAGTCCTAACAGTAGGACTTTTGAGATTCTCATGGGATCCTTTATTGTAAAGGACCGAGTTGATGAAGCTGTTATTGTTTTCGATGAAATGCTTGATCTTGGTTGTCAGCCTGATTTGAGTTTTTATTCCTGCATAATACCTTTGTTTTGTCAGGAAAATAAACTGGAGGAGGGAATTAGATTGTTTGAAATGATGAGAACTTCTAATTTTACGGCAGATTCATTGGTTTATGAGGTTCTGTTGCAGTCTTTATGCAAGAACCTTAGATTGGATGATGCGATAAAGGTTTTAGAAGAGATGATGGAAACTGGGTTGACGCCGCCAAATAATGTCTTTGCAATTGTAGTATATGTGTTTTGTAAATTGGGGAAGGTAGACGACGCGATGAAGTTCTTGGAAGATAAGCAAATCATGGAAACTTCTGCCTGCAACGTACTGCTTGAAGTTTGCTGCAGTACTGGAAAATTTCTTAAGGCAGAAGATTTACTTGTGGAAATGTCTGAAAGAAATGTGGCTGATTGTAATTCTTGGAATATTGTTATCAGATGGCTCTCCGAGCACGCAAGGATTAGGGAAGTGCGTGAACTTCTTGGTAGAATGGTGGTTTCTTGTTCTCTTCCCGACTGTGACACATACTCGGCTCTAGTCGTTTGCCACTGCAAAATGAGCAACTACAGAAATGCTATGGATATATTTGATCATATTCGTGCCAAAAGCTGGGTTTTGGATCATACATCTTACTCTGAGCTTGTCAAAGGCCTTTGCCTAGGGGAAATGACTCATGAGGCTACTGAAGTGTTCTGTTACATGTCTAGTAATAGATGTTCGATCGAGCCTTCCTCGTTCAATATGTTGATCAAGGATTTGTGTGAGACAGGAAAGGTTGACGAAGCAGCAAGGATACAACAATTGGCCTATTATTCCGGTACTTCTTCTACCAGTTCAACTTACTCAACTATTATGCTTGGATTGTCAAAATTAGACAAGGTAAAAGATCTGCTGATAGTCCTCTCAAAAATGCTGGTGGAGGGTTGCAGTCTTGATTTGGATGCGTACTGCGTGCTCATACAAAGCATGAGTGTGCAGAATCGAATGAAAGAATGCTTATTGCTTTTCAATATGATGGTCGATGAGGGTCTTATACCTGATTCTGAGAGACTATTTAATCTACTCTCTTGTATAGCCAACTATTCTCAGTTGCACATGATTTCGTGTTCAATAAATAAACTTACTTCTGATAGTAACAGGCCGGACTGTGACAATTCTTCTGTAGATACCGTTAGCAACATCCTTGCAGAGGGCTTAGATGCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

671

Amino Acids

75.45

Weight (kDa)

4.94

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 137 - 226 3.2e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 189 - 246 7.3e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 199 - 246 9.1e-11 PPR repeat family
PPR_long PF17177 254 - 416 8.4e-06 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 265 - 363 6.5e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 266 - 298 7.5e-06 PPR repeat
PPR_2 PF13041 269 - 318 1.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 464
AccB1I GGYRCC 2 cut(s) 403, 518
AccB7I CCANNNNNTGG 1 cut(s) 1413
AccBSI CCGCTC 1 cut(s) 642
AccI GTMKAC 1 cut(s) 1071
AccII CGCG 1 cut(s) 1079
AciI CCGC 4 cut(s) 282, 642, 646, 1016
AclWI GGATC 3 cut(s) 726, 739, 1431
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 3 cut(s) 133, 463, 1160
AcuI CTGAAG 1 cut(s) 1509
AcyI GRCGYC 1 cut(s) 1013
AfaI GTAC 5 cut(s) 183, 1131, 1153, 1624, 1751
AfiI CCNNNNNNNGG 6 cut(s) 49, 205, 529, 964, 1413, 1993
AflII CTTAAG 1 cut(s) 1166
AflIII ACRYGT 1 cut(s) 1503
AjuI GAANNNNNNNTTGG 4 cut(s) 302, 334, 947, 979
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
AluBI AGCT 6 cut(s) 13, 178, 228, 764, 1413, 1444
AluI AGCT 6 cut(s) 13, 178, 228, 764, 1413, 1444
Alw21I GWGCWC 3 cut(s) 230, 1257, 1761
Alw26I GTCTC 3 cut(s) 491, 1562, 1846
AlwI GGATC 3 cut(s) 726, 739, 1431
AoxI GGCC 5 cut(s) 485, 643, 1453, 1608, 1943
ApeKI GCWGC 2 cut(s) 1146, 1589
ApoI RAATTY 3 cut(s) 133, 463, 1160
AspA2I CCTAGG 1 cut(s) 1462
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 2 cut(s) 124, 748
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 376
AsuII TTCGAA 1 cut(s) 59
AvaII GGWCC 2 cut(s) 124, 748
AvrII CCTAGG 1 cut(s) 1462
BaeGI GKGCMC 1 cut(s) 523
BamHI GGATCC 1 cut(s) 731
BanI GGYRCC 2 cut(s) 403, 518
BanII GRGCYC 1 cut(s) 230
Bbv12I GWGCWC 3 cut(s) 230, 1257, 1761
BbvI GCAGC 2 cut(s) 1133, 1601
BccI CCATC 5 cut(s) 334, 365, 991, 1236, 1813
BceAI ACGGC 2 cut(s) 597, 924
BciVI GTATCC 1 cut(s) 1590
BclI TGATCA 3 cut(s) 664, 1393, 1551
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 3 cut(s) 491, 1562, 1846
BfaI CTAG 3 cut(s) 1340, 1463, 1509
BfmI CTRYAG 3 cut(s) 1147, 1369, 1965
BfoI RGCGCY 1 cut(s) 398
BfrI CTTAAG 1 cut(s) 1166
BfuAI ACCTGC 1 cut(s) 464
BfuI GTATCC 1 cut(s) 1590
BglII AGATCT 1 cut(s) 1690
BisI GCNGC 5 cut(s) 643, 646, 1016, 1147, 1590
BlnI CCTAGG 1 cut(s) 1462
BlpI GCTNAGC 1 cut(s) 229
BlsI GCNGC 5 cut(s) 644, 647, 1017, 1148, 1591
BmcAI AGTACT 1 cut(s) 1153
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 2 cut(s) 124, 748
BmgT120I GGNCC 2 cut(s) 124, 748
BmiI GGNNCC 4 cut(s) 126, 405, 520, 733
BmrFI CCNGG 1 cut(s) 200
BmrI ACTGGG 2 cut(s) 323, 1014
BmsI GCATC 3 cut(s) 961, 1735, 1993
BmuI ACTGGG 2 cut(s) 323, 1014
BplI GAGNNNNNCTC 2 cut(s) 1520, 1552
BpmI CTGGAG 1 cut(s) 881
Bpu1102I GCTNAGC 1 cut(s) 229
Bpu14I TTCGAA 1 cut(s) 59
BpuMI CCSGG 1 cut(s) 200
BsaAI YACGTR 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 1013
BsaJI CCNNGG 1 cut(s) 1462
BsaWI WCCGGW 2 cut(s) 184, 1619
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 205, 529, 964, 1413, 1993
Bse1I ACTGG 7 cut(s) 16, 160, 318, 864, 1009, 1159, 1635
Bse3DI GCAATG 2 cut(s) 427, 453
BseDI CCNNGG 1 cut(s) 1462
BseGI GGATG 4 cut(s) 137, 973, 1750, 1983
BseLI CCNNNNNNNGG 6 cut(s) 49, 205, 529, 964, 1413, 1993
BseMI GCAATG 2 cut(s) 427, 453
BseMII CTCAG 4 cut(s) 243, 1431, 1839, 1904
BseNI ACTGG 7 cut(s) 16, 160, 318, 864, 1009, 1159, 1635
BseRI GAGGAG 2 cut(s) 350, 878
BseSI GKGCMC 1 cut(s) 523
BseX3I CGGCCG 1 cut(s) 643
BseXI GCAGC 2 cut(s) 1133, 1601
BseYI CCCAGC 1 cut(s) 1413
BsgI GTGCAG 3 cut(s) 60, 70, 1799
Bsh1236I CGCG 1 cut(s) 1079
Bsh1285I CGRYCG 2 cut(s) 646, 1527
BshFI GGCC 5 cut(s) 487, 645, 1455, 1610, 1945
BshNI GGYRCC 2 cut(s) 403, 518
BsiEI CGRYCG 2 cut(s) 646, 1527
BsiHKAI GWGCWC 3 cut(s) 230, 1257, 1761
BsiSI CCGG 4 cut(s) 185, 199, 1620, 1946
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 6 cut(s) 49, 205, 529, 964, 1413, 1993
BsmAI GTCTC 3 cut(s) 491, 1562, 1846
BsmFI GGGAC 1 cut(s) 363
BsmI GAATGC 2 cut(s) 67, 1802
BsnI GGCC 5 cut(s) 487, 645, 1455, 1610, 1945
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 4 cut(s) 230, 523, 1257, 1761
Bsp143I GATC 8 cut(s) 664, 731, 790, 1393, 1423, 1524, 1551, 1690
Bsp1720I GCTNAGC 1 cut(s) 229
BspACI CCGC 4 cut(s) 282, 642, 646, 1016
BspANI GGCC 5 cut(s) 487, 645, 1455, 1610, 1945
BspCNI CTCAG 4 cut(s) 242, 1432, 1840, 1903
BspFNI CGCG 1 cut(s) 1079
BspHI TCATGA 1 cut(s) 1477
BspLI GGNNCC 4 cut(s) 126, 405, 520, 733
BspMAI CTGCAG 1 cut(s) 1151
BspMI ACCTGC 1 cut(s) 464
BspPI GGATC 3 cut(s) 726, 739, 1431
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 403, 518
BspTI CTTAAG 1 cut(s) 1166
BsrBI CCGCTC 1 cut(s) 642
BsrDI GCAATG 2 cut(s) 427, 453
BsrI ACTGG 7 cut(s) 16, 160, 318, 864, 1009, 1159, 1635
BssECI CCNNGG 1 cut(s) 1462
BssMI GATC 8 cut(s) 664, 731, 790, 1393, 1423, 1524, 1551, 1690
BssNI GRCGYC 1 cut(s) 1013
BssT1I CCWWGG 1 cut(s) 1462
Bst4CI ACNGT 5 cut(s) 270, 707, 1322, 1952, 1975
Bst6I CTCTTC 3 cut(s) 384, 984, 1317
BstACI GRCGYC 1 cut(s) 1013
BstAFI CTTAAG 1 cut(s) 1166
BstBAI YACGTR 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 67, 1123, 1257, 1757
BstDEI CTNAG 6 cut(s) 229, 959, 1440, 1848, 1890, 1999
BstENI CCTNNNNNAGG 2 cut(s) 527, 1991
BstF5I GGATG 4 cut(s) 137, 973, 1750, 1983
BstFNI CGCG 1 cut(s) 1079
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 8 cut(s) 667, 734, 793, 1396, 1426, 1527, 1554, 1693
BstMAI GTCTC 3 cut(s) 491, 1562, 1846
BstMBI GATC 8 cut(s) 664, 731, 790, 1393, 1423, 1524, 1551, 1690
BstMCI CGRYCG 2 cut(s) 646, 1527
BstMWI GCNNNNNNNGC 4 cut(s) 10, 401, 1252, 1362
BstNSI RCATGY 2 cut(s) 69, 1507
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 3 cut(s) 1147, 1369, 1965
BstSLI GKGCMC 1 cut(s) 523
BstSNI TACGTA 1 cut(s) 291
BstUI CGCG 1 cut(s) 1079
BstV1I GCAGC 2 cut(s) 1133, 1601
BstX2I RGATCY 2 cut(s) 731, 1690
BstYI RGATCY 2 cut(s) 731, 1690
BstZI CGGCCG 1 cut(s) 643
BsuI GTATCC 1 cut(s) 1590
BsuRI GGCC 5 cut(s) 487, 645, 1455, 1610, 1945
BtgZI GCGATG 1 cut(s) 1094
BtsCI GGATG 4 cut(s) 137, 973, 1750, 1983
BtsI GCAGTG 2 cut(s) 240, 1351
BtsIMutI CAGTG 3 cut(s) 213, 240, 1351
BveI ACCTGC 1 cut(s) 464
Cac8I GCNNGC 4 cut(s) 67, 1123, 1257, 1757
CciI TCATGA 1 cut(s) 1477
CciNI GCGGCCGC 1 cut(s) 643
CfoI GCGC 1 cut(s) 397
Cfr13I GGNCC 2 cut(s) 124, 748
CseI GACGC 2 cut(s) 1021, 1085
Csp6I GTAC 5 cut(s) 182, 1130, 1152, 1623, 1750
CviAII CATG 9 cut(s) 66, 592, 727, 1108, 1478, 1504, 1771, 1900, 2010
CviQI GTAC 5 cut(s) 182, 1130, 1152, 1623, 1750
DdeI CTNAG 6 cut(s) 229, 959, 1440, 1848, 1890, 1999
DpnI GATC 8 cut(s) 666, 733, 792, 1395, 1425, 1526, 1553, 1692
DpnII GATC 8 cut(s) 664, 731, 790, 1393, 1423, 1524, 1551, 1690
EaeI YGGCCR 1 cut(s) 643
EagI CGGCCG 1 cut(s) 643
Eam1104I CTCTTC 3 cut(s) 384, 984, 1317
EarI CTCTTC 3 cut(s) 384, 984, 1317
Ecl136II GAGCTC 1 cut(s) 228
EclXI CGGCCG 1 cut(s) 643
Eco105I TACGTA 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 1462
Eco147I AGGCCT 2 cut(s) 487, 1455
Eco24I GRGCYC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 124, 748
Eco52I CGGCCG 1 cut(s) 643
Eco53kI GAGCTC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 1509
EcoICRI GAGCTC 1 cut(s) 228
EcoNI CCTNNNNNAGG 2 cut(s) 527, 1991
EcoO109I RGGNCCY 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 1462
EcoT22I ATGCAT 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 230
ErhI CCWWGG 1 cut(s) 1462
FaeI CATG 9 cut(s) 69, 595, 730, 1111, 1481, 1507, 1774, 1903, 2013
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 1167, 1199
FaqI GGGAC 1 cut(s) 363
FatI CATG 9 cut(s) 65, 591, 726, 1107, 1477, 1503, 1770, 1899, 2009
FbaI TGATCA 3 cut(s) 664, 1393, 1551
FblI GTMKAC 1 cut(s) 1071
Fnu4HI GCNGC 5 cut(s) 643, 646, 1016, 1147, 1590
FokI GGATG 4 cut(s) 124, 980, 1757, 1970
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 5 cut(s) 643, 646, 1016, 1147, 1590
FspBI CTAG 3 cut(s) 1340, 1463, 1509
GlaI GCGC 1 cut(s) 396
GluI GCNGC 5 cut(s) 643, 646, 1016, 1147, 1590
GsaI CCCAGC 1 cut(s) 1417
GsuI CTGGAG 1 cut(s) 881
HaeII RGCGCY 1 cut(s) 398
HaeIII GGCC 5 cut(s) 487, 645, 1455, 1610, 1945
HapII CCGG 4 cut(s) 185, 199, 1620, 1946
HgaI GACGC 2 cut(s) 1021, 1085
HhaI GCGC 1 cut(s) 397
Hin1I GRCGYC 1 cut(s) 1013
Hin1II CATG 9 cut(s) 69, 595, 730, 1111, 1481, 1507, 1774, 1903, 2013
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 2 cut(s) 1011, 1582
HindII GTYRAC 2 cut(s) 1011, 1582
HinfI GANTC 9 cut(s) 39, 172, 588, 653, 721, 914, 1474, 1783, 1844
HpaII CCGG 4 cut(s) 185, 199, 1620, 1946
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 4 cut(s) 1011, 1072, 1280, 1582
Hpy188I TCNGA 8 cut(s) 142, 300, 1198, 1241, 1252, 1441, 1849, 1933
Hpy188III TCNNGA 9 cut(s) 111, 122, 206, 380, 548, 848, 1316, 1478, 1736
Hpy8I GTNNAC 4 cut(s) 1011, 1072, 1280, 1582
Hpy99I CGWCG 1 cut(s) 1079
HpyAV CCTTC 6 cut(s) 55, 232, 498, 523, 1060, 1542
HpyCH4III ACNGT 5 cut(s) 270, 707, 1322, 1952, 1975
HpyCH4IV ACGT 2 cut(s) 290, 1128
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 401, 1252, 1362
HpyF3I CTNAG 6 cut(s) 229, 959, 1440, 1848, 1890, 1999
HpySE526I ACGT 2 cut(s) 290, 1128
Hsp92I GRCGYC 1 cut(s) 1013
Hsp92II CATG 9 cut(s) 69, 595, 730, 1111, 1481, 1507, 1774, 1903, 2013
HspAI GCGC 1 cut(s) 395
Ksp22I TGATCA 3 cut(s) 664, 1393, 1551
Kzo9I GATC 8 cut(s) 664, 731, 790, 1393, 1423, 1524, 1551, 1690
LmnI GCTCC 1 cut(s) 18
Lsp1109I GCAGC 2 cut(s) 1133, 1601
LweI GCATC 3 cut(s) 961, 1735, 1993
MaeI CTAG 3 cut(s) 1340, 1463, 1509
MaeII ACGT 2 cut(s) 290, 1128
MaeIII GTNAC 5 cut(s) 577, 1322, 1499, 1937, 1952
MalI GATC 8 cut(s) 666, 733, 792, 1395, 1425, 1526, 1553, 1692
MbiI CCGCTC 1 cut(s) 642
MboI GATC 8 cut(s) 664, 731, 790, 1393, 1423, 1524, 1551, 1690
MfeI CAATTG 3 cut(s) 492, 1035, 1604
MflI RGATCY 2 cut(s) 731, 1690
MhlI GDGCHC 4 cut(s) 230, 523, 1257, 1761
MlyI GAGTC 2 cut(s) 662, 1468
MmeI TCCRAC 2 cut(s) 165, 615
Mph1103I ATGCAT 1 cut(s) 67
MseI TTAA 7 cut(s) 23, 194, 273, 437, 504, 1167, 1860
MslI CAYNNNNRTG 3 cut(s) 101, 1401, 1775
MspCI CTTAAG 1 cut(s) 1166
MspI CCGG 4 cut(s) 185, 199, 1620, 1946
MspR9I CCNGG 1 cut(s) 200
MunI CAATTG 3 cut(s) 492, 1035, 1604
Mva1269I GAATGC 2 cut(s) 67, 1802
MvnI CGCG 1 cut(s) 1079
MwoI GCNNNNNNNGC 4 cut(s) 10, 401, 1252, 1362
NciI CCSGG 1 cut(s) 200
NdeII GATC 8 cut(s) 664, 731, 790, 1393, 1423, 1524, 1551, 1690
NlaIII CATG 9 cut(s) 69, 595, 730, 1111, 1481, 1507, 1774, 1903, 2013
NlaIV GGNNCC 4 cut(s) 126, 405, 520, 733
NmeAIII GCCGAG 1 cut(s) 1313
NmuCI GTSAC 3 cut(s) 577, 1322, 1952
NotI GCGGCCGC 1 cut(s) 643
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 2 cut(s) 69, 1507
NspV TTCGAA 1 cut(s) 59
PaeI GCATGC 1 cut(s) 69
PagI TCATGA 1 cut(s) 1477
PaqCI CACCTGC 1 cut(s) 464
PceI AGGCCT 2 cut(s) 487, 1455
PciI ACATGT 1 cut(s) 1503
PctI GAATGC 2 cut(s) 67, 1802
PfeI GAWTC 7 cut(s) 39, 172, 588, 721, 914, 1783, 1844
PflMI CCANNNNNTGG 1 cut(s) 1413
PkrI GCNGC 5 cut(s) 644, 647, 1017, 1148, 1591
Ple19I CGATCG 1 cut(s) 1527
PleI GAGTC 2 cut(s) 661, 1468
PpsI GAGTC 2 cut(s) 661, 1468
Ppu21I YACGTR 1 cut(s) 291
PpuMI RGGWCCY 1 cut(s) 124
PscI ACATGT 1 cut(s) 1503
Psp124BI GAGCTC 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 124
PspFI CCCAGC 1 cut(s) 1413
PspN4I GGNNCC 4 cut(s) 126, 405, 520, 733
PspPI GGNCC 2 cut(s) 124, 748
PspPPI RGGWCCY 1 cut(s) 124
PsrI GAACNNNNNNTAC 2 cut(s) 1504, 1536
PstI CTGCAG 1 cut(s) 1151
PsuI RGATCY 2 cut(s) 731, 1690
PvuI CGATCG 1 cut(s) 1527
RsaI GTAC 5 cut(s) 183, 1131, 1153, 1624, 1751
RsaNI GTAC 5 cut(s) 182, 1130, 1152, 1623, 1750
RseI CAYNNNNRTG 3 cut(s) 101, 1401, 1775
SacI GAGCTC 1 cut(s) 230
SaqAI TTAA 7 cut(s) 23, 194, 273, 437, 504, 1167, 1860
SatI GCNGC 5 cut(s) 643, 646, 1016, 1147, 1590
Sau3AI GATC 8 cut(s) 664, 731, 790, 1393, 1423, 1524, 1551, 1690
Sau96I GGNCC 2 cut(s) 124, 748
ScaI AGTACT 1 cut(s) 1153
SchI GAGTC 2 cut(s) 662, 1468
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 4 cut(s) 230, 523, 1257, 1761
SfaNI GCATC 3 cut(s) 961, 1735, 1993
SfcI CTRYAG 3 cut(s) 1147, 1369, 1965
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 2 cut(s) 124, 748
SmiMI CAYNNNNRTG 3 cut(s) 101, 1401, 1775
SmlI CTYRAG 1 cut(s) 1166
SmoI CTYRAG 1 cut(s) 1166
SnaBI TACGTA 1 cut(s) 291
SphI GCATGC 1 cut(s) 69
SseBI AGGCCT 2 cut(s) 487, 1455
SsiI CCGC 4 cut(s) 282, 642, 646, 1016
SspI AATATT 1 cut(s) 1231
SspMI CTAG 3 cut(s) 1340, 1463, 1509
SstI GAGCTC 1 cut(s) 230
StuI AGGCCT 2 cut(s) 487, 1455
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 1462
TaaI ACNGT 5 cut(s) 270, 707, 1322, 1952, 1975
TaiI ACGT 2 cut(s) 293, 1131
TaqI TCGA 9 cut(s) 59, 245, 387, 547, 777, 1523, 1527, 1786, 1824
TaqII GACCGA 1 cut(s) 765
TatI WGTACW 1 cut(s) 1151
TauI GCSGC 3 cut(s) 645, 648, 1018
TfiI GAWTC 7 cut(s) 39, 172, 588, 721, 914, 1783, 1844
Tru1I TTAA 7 cut(s) 23, 194, 273, 437, 504, 1167, 1860
Tru9I TTAA 7 cut(s) 23, 194, 273, 437, 504, 1167, 1860
TscAI CASTG 3 cut(s) 220, 240, 1358
TseFI GTSAC 3 cut(s) 577, 1322, 1952
TseI GCWGC 2 cut(s) 1146, 1589
Tsp45I GTSAC 3 cut(s) 577, 1322, 1952
TspDTI ATGAA 7 cut(s) 126, 695, 774, 795, 906, 1097, 1805
TspRI CASTG 3 cut(s) 220, 240, 1358
Van91I CCANNNNNTGG 1 cut(s) 1413
Vha464I CTTAAG 1 cut(s) 1166
VpaK11BI GGWCC 2 cut(s) 124, 748
XagI CCTNNNNNAGG 2 cut(s) 527, 1991
XapI RAATTY 3 cut(s) 133, 463, 1160
XceI RCATGY 2 cut(s) 69, 1507
XmaJI CCTAGG 1 cut(s) 1462
XmiI GTMKAC 1 cut(s) 1071
XspI CTAG 3 cut(s) 1340, 1463, 1509
ZrmI AGTACT 1 cut(s) 1153
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.