Rorug03G0027500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008146
Physical Location & Seq
Forward (+)
2126181 .. 2126758
578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0027500.1

Sequence Viewer

Length: 456 bp
ATGAGGAAATTGATTATTGTTGGGTTTGTGAAGCAGGTTAGTATTCCACCTAGTGATATATACAAAGCCCTAGCCGTTTACATCCAGTCTCCCGGCTCCGCCTTCCTCTTCTGCGGCGCCGTCACCGTCGCTCGGCCCTCGGCGGTGCTCTCCCTCCCCTGGCCGGAGCCCGGTGGTCAGCTCCAACTCACCGCCGACGCCGCCCCACTCTCCGCCAAGATCGGGGTCTCGGTCGAGGACCTGGCCACGCTGCCGTCTTTGGATGTCACCGCCGAGAAGCGAATTGAGCGTTTGGCGATGAAGGTTGGGGAGAATCTGTTCAATTTTATGCAGTCGTTTTGTGGTGTCGATGGCTCCAAGCTGGTGGTGCCCATGGATATATTGGATCGATGGTTCAAGAAGTTCCAGGAGAGGGCTAAGCGAGACCCCGAGTACTTGAAGGGCTTCGTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

151

Amino Acids

16.57

Weight (kDa)

8.93

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hikeshi-like_C PF21057 95 - 147 1.3e-12 Hikeshi-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 25
AccB1I GGYRCC 2 cut(s) 116, 367
AciI CCGC 7 cut(s) 99, 114, 143, 192, 201, 213, 270
AclWI GGATC 1 cut(s) 393
AcoI YGGCCR 2 cut(s) 161, 243
AcyI GRCGYC 2 cut(s) 117, 198
AdeI CACNNNGTG 1 cut(s) 53
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 6 cut(s) 132, 159, 163, 170, 222, 412
AgsI TTSAA 3 cut(s) 322, 397, 439
AjnI CCWGG 3 cut(s) 158, 240, 405
AloI GAACNNNNNNTCC 4 cut(s) 302, 334, 377, 409
AluBI AGCT 2 cut(s) 181, 361
AluI AGCT 2 cut(s) 181, 361
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 3 cut(s) 93, 232, 417
AlwI GGATC 1 cut(s) 393
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 3 cut(s) 134, 161, 243
ApeKI GCWGC 1 cut(s) 250
Asp700I GAANNNNTTC 2 cut(s) 317, 443
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 135, 238
AsuC2I CCSGG 2 cut(s) 93, 171
AsuHPI GGTGA 3 cut(s) 115, 181, 259
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 372
BalI TGGCCA 1 cut(s) 245
BanI GGYRCC 2 cut(s) 116, 367
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 237
BccI CCATC 2 cut(s) 344, 384
BceAI ACGGC 3 cut(s) 59, 104, 238
BciT130I CCWGG 3 cut(s) 160, 242, 407
BcnI CCSGG 2 cut(s) 93, 171
BcoDI GTCTC 3 cut(s) 93, 232, 417
BfaI CTAG 2 cut(s) 51, 71
BfoI RGCGCY 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 25
BisI GCNGC 3 cut(s) 115, 201, 251
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 3 cut(s) 116, 202, 252
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 5 cut(s) 93, 160, 171, 242, 407
Bme18I GGWCC 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 2 cut(s) 135, 238
BmiI GGNNCC 5 cut(s) 97, 118, 168, 355, 369
BmrFI CCNGG 5 cut(s) 93, 160, 171, 242, 407
Bpu1102I GCTNAGC 1 cut(s) 417
BpuMI CCSGG 2 cut(s) 93, 171
Bsa29I ATCGAT 1 cut(s) 388
BsaHI GRCGYC 2 cut(s) 117, 198
BsaI GGTCTC 2 cut(s) 232, 417
BsaJI CCNNGG 3 cut(s) 138, 158, 372
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 6 cut(s) 132, 159, 163, 170, 222, 412
Bse1I ACTGG 1 cut(s) 85
BseBI CCWGG 3 cut(s) 160, 242, 407
BseCI ATCGAT 1 cut(s) 388
BseDI CCNNGG 3 cut(s) 138, 158, 372
BseGI GGATG 2 cut(s) 81, 268
BseLI CCNNNNNNNGG 6 cut(s) 132, 159, 163, 170, 222, 412
BseNI ACTGG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 1 cut(s) 237
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 3 cut(s) 136, 163, 245
BshNI GGYRCC 2 cut(s) 116, 367
BshVI ATCGAT 1 cut(s) 388
BsiEI CGRYCG 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 3 cut(s) 93, 164, 171
BslI CCNNNNNNNGG 6 cut(s) 132, 159, 163, 170, 222, 412
BsmAI GTCTC 3 cut(s) 93, 232, 417
BsnI GGCC 3 cut(s) 136, 163, 245
Bso31I GGTCTC 2 cut(s) 232, 417
BsoBI CYCGRG 1 cut(s) 428
Bsp1286I GDGCHC 3 cut(s) 150, 171, 372
Bsp143I GATC 2 cut(s) 219, 385
Bsp1720I GCTNAGC 1 cut(s) 417
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 7 cut(s) 99, 114, 143, 192, 201, 213, 270
BspANI GGCC 3 cut(s) 136, 163, 245
BspDI ATCGAT 1 cut(s) 388
BspLI GGNNCC 5 cut(s) 97, 118, 168, 355, 369
BspMI ACCTGC 1 cut(s) 25
BspPI GGATC 1 cut(s) 393
BspT107I GGYRCC 2 cut(s) 116, 367
BspTNI GGTCTC 2 cut(s) 232, 417
BsrI ACTGG 1 cut(s) 85
BssECI CCNNGG 3 cut(s) 138, 158, 372
BssMI GATC 2 cut(s) 219, 385
BssNI GRCGYC 2 cut(s) 117, 198
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 3 cut(s) 160, 242, 407
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 2 cut(s) 117, 198
BstDEI CTNAG 1 cut(s) 417
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 81, 268
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 2 cut(s) 222, 388
BstMAI GTCTC 3 cut(s) 93, 232, 417
BstMBI GATC 2 cut(s) 219, 385
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 3 cut(s) 200, 286, 367
BstNI CCWGG 3 cut(s) 160, 242, 407
BstSCI CCNGG 5 cut(s) 91, 158, 169, 240, 405
BstSLI GKGCMC 1 cut(s) 372
BstV1I GCAGC 1 cut(s) 237
BstXI CCANNNNNNTGG 1 cut(s) 364
Bsu15I ATCGAT 1 cut(s) 388
BsuRI GGCC 3 cut(s) 136, 163, 245
BsuTUI ATCGAT 1 cut(s) 388
BtgI CCRYGG 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 311
BtsCI GGATG 2 cut(s) 81, 268
BveI ACCTGC 1 cut(s) 25
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 135, 238
ClaI ATCGAT 1 cut(s) 388
CseI GACGC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 433
CviAII CATG 1 cut(s) 373
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 1 cut(s) 417
DinI GGCGCC 1 cut(s) 118
DpnI GATC 2 cut(s) 221, 387
DpnII GATC 2 cut(s) 219, 385
DraIII CACNNNGTG 1 cut(s) 53
EaeI YGGCCR 2 cut(s) 161, 243
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 2 cut(s) 88, 202
Eco130I CCWWGG 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 232, 417
Eco47I GGWCC 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 3 cut(s) 158, 240, 405
EcoT14I CCWWGG 1 cut(s) 372
EcoT38I GRGCYC 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 1 cut(s) 376
FaiI YATR 5 cut(s) 59, 61, 329, 374, 380
FatI CATG 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 115, 201, 251
FokI GGATG 2 cut(s) 68, 275
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 3 cut(s) 115, 201, 251
FspBI CTAG 2 cut(s) 51, 71
GlaI GCGC 1 cut(s) 118
GluI GCNGC 3 cut(s) 115, 201, 251
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 3 cut(s) 136, 163, 245
HapII CCGG 3 cut(s) 93, 164, 171
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 119
Hin1I GRCGYC 2 cut(s) 117, 198
Hin1II CATG 1 cut(s) 376
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HinfI GANTC 1 cut(s) 313
HpaII CCGG 3 cut(s) 93, 164, 171
HphI GGTGA 3 cut(s) 115, 181, 259
Hpy166II GTNNAC 1 cut(s) 79
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 131, 200
HpyAV CCTTC 3 cut(s) 112, 295, 433
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 1 cut(s) 331
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 286, 367
HpyF3I CTNAG 1 cut(s) 417
Hsp92I GRCGYC 2 cut(s) 117, 198
Hsp92II CATG 1 cut(s) 376
HspAI GCGC 1 cut(s) 117
KasI GGCGCC 1 cut(s) 116
Kzo9I GATC 2 cut(s) 219, 385
LmnI GCTCC 4 cut(s) 101, 166, 186, 359
Lsp1109I GCAGC 1 cut(s) 237
MaeI CTAG 2 cut(s) 51, 71
MaeIII GTNAC 2 cut(s) 121, 265
MalI GATC 2 cut(s) 221, 387
MboI GATC 2 cut(s) 219, 385
MboII GAAGA 1 cut(s) 100
MhlI GDGCHC 3 cut(s) 150, 171, 372
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 3 cut(s) 8, 282, 322
MluNI TGGCCA 1 cut(s) 245
Mly113I GGCGCC 1 cut(s) 117
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 5 cut(s) 116, 148, 164, 229, 405
Mox20I TGGCCA 1 cut(s) 245
MroXI GAANNNNTTC 2 cut(s) 317, 443
MscI TGGCCA 1 cut(s) 245
Msp20I TGGCCA 1 cut(s) 245
MspI CCGG 3 cut(s) 93, 164, 171
MspR9I CCNGG 5 cut(s) 93, 160, 171, 242, 407
MvaI CCWGG 3 cut(s) 160, 242, 407
MwoI GCNNNNNNNGC 3 cut(s) 200, 286, 367
NarI GGCGCC 1 cut(s) 117
NciI CCSGG 2 cut(s) 93, 171
NcoI CCATGG 1 cut(s) 372
NdeII GATC 2 cut(s) 219, 385
NlaIII CATG 1 cut(s) 376
NlaIV GGNNCC 5 cut(s) 97, 118, 168, 355, 369
NmeAIII GCCGAG 3 cut(s) 112, 119, 298
NmuCI GTSAC 2 cut(s) 121, 265
PdmI GAANNNNTTC 2 cut(s) 317, 443
PfeI GAWTC 1 cut(s) 313
PfoI TCCNGGA 1 cut(s) 405
PkrI GCNGC 3 cut(s) 116, 202, 252
PluTI GGCGCC 1 cut(s) 120
PpuMI RGGWCCY 1 cut(s) 238
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 3 cut(s) 158, 240, 405
PspGI CCWGG 3 cut(s) 158, 240, 405
PspN4I GGNNCC 5 cut(s) 97, 118, 168, 355, 369
PspPI GGNCC 2 cut(s) 135, 238
PspPPI RGGWCCY 1 cut(s) 238
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
SatI GCNGC 3 cut(s) 115, 201, 251
Sau3AI GATC 2 cut(s) 219, 385
Sau96I GGNCC 2 cut(s) 135, 238
ScaI AGTACT 1 cut(s) 434
ScrFI CCNGG 5 cut(s) 93, 160, 171, 242, 407
SduI GDGCHC 3 cut(s) 150, 171, 372
SetI ASST 6 cut(s) 39, 52, 183, 243, 306, 363
SfoI GGCGCC 1 cut(s) 118
SinI GGWCC 1 cut(s) 238
Sse9I AATT 3 cut(s) 8, 282, 322
SsiI CCGC 7 cut(s) 99, 114, 143, 192, 201, 213, 270
SspDI GGCGCC 1 cut(s) 116
SspMI CTAG 2 cut(s) 51, 71
StyD4I CCNGG 5 cut(s) 91, 158, 169, 240, 405
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 127
TaqI TCGA 3 cut(s) 234, 348, 388
TaqII GACCGA 1 cut(s) 220
TasI AATT 3 cut(s) 8, 282, 322
TatI WGTACW 1 cut(s) 432
TauI GCSGC 2 cut(s) 117, 203
TfiI GAWTC 1 cut(s) 313
TseFI GTSAC 2 cut(s) 121, 265
TseI GCWGC 1 cut(s) 250
Tsp45I GTSAC 2 cut(s) 121, 265
TspDTI ATGAA 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 379
XmnI GAANNNNTTC 2 cut(s) 317, 443
XspI CTAG 2 cut(s) 51, 71
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.