MD12G1204700.v1.1

NHL repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008360
Physical Location & Seq
Reverse (-)
28548726 .. 28556932
8207 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1204700.v1.1.491

Sequence Viewer

Length: 3246 bp
ATGGCCATGAAACTACTATCGCCACCCACTTCTCTCTCACAGCCCACCAAGCTCTGCTCTTTATACGGCAGCTCGAAAGGATTCAAACCCATTTCAGTCTCGACCCGACTCTTCCAGTGGAGGCCGAAACGGTTCGACTTCTCGAAGAAAATGGTTGTGAAAGCTGGTGTGAAAGTGGAGGAGAAAAATGTGCAAGAATCATCGGGAAGTGAGTGGGGGAAAGTGTCTGCTGTGCTGTTTGACATGGATGGAGTTCTCTGCGACAGCGAAGAGCCGTCTCGATTGGCCGCAGTCGACGTTTTCGCCGAGATGGGGGTGCAGGTCACGGTGGAGGACTTTGTGCCATTCATGGGAACTGAAGCGAACTTCTTAGGAGGTGTTGCTTCCGTTAAGGGGGTAAAGGGATTCGATCCTGAGGCAGCGAAGAAGAGGTTCTTTGAGATATATTTGGATAAGTATGCAAAACCAAATTCTGGAATAGGATTTCCAGGTGCCCTTGAACTTATTACTCAGTGTAAAGAAAAAGGCCTTAAAGTTGCTGTTGCATCTAGTGCTGATCGAATCAAGGTTAATGCAAACCTAGCTGCTGCAAATCTACCGTTGTCAATGTTTGATGCTATTGTGTCAGCAGATGCTTTTGAGAAACTGAAACCTGCTCCTGATATCTTCTTGGCTGCATCCAAAATCTTGGATGTCCCGACTAGTGAGTGTATTGTTATTGAAGATGCATTAGCTGGAGTGCAGGCCGCCAAAGCTGCCAAAATGAAATGCATAGCAGTCAAAACTACTTTATCAGAGGCGGAGCTGAAGGCTGCTGGTCCATCCCTAATCCGAAAAGAAATAGGAAATATTTCCCTTGATGATATTCTCGGTGGTGGCTCTGGCTATAATGGGACGATTCAGGGACCTCAAGTTTCTTATATGTCATCTCAAAACACAACAGAAAAGGTCACAGAAATAAATTCTGAGTTAATACAGAAAACTGGTGCTTCCAATGGTGGGGTTTTCTCAGATGGAAGGGTTCTTCGGCGAGATATAGTGAGATATGGAAGCTTGGGGATTTCTTTGTCTTGTCTTGCCTTCGCCATCTCAAACTGGAAGGCAATGCAATATACGTCACCTCAAGCTATTTGGAATGTGATATTTGGGATTAACCAGCCATCTCTTAAACAGAAGGAAGGTGAATCAAGATCTGAAAGAATCCAACAATTTGTGAACTACATATCTGACCTGGAAACCAGAGGAACTGCTCCTATCGTGCCAGAATTTCCGGCAAAACTTGATTGGTTGAATACAGCTCCCATTACGCTTAGCAGGGATCTGAAAGGAAAAGTTGTTCTGCTAGATTTTTGGACCTATTGTTGTATAAACTGTATGCATGTACTGCCGGATCTAGAGTTTCTGGAGAAAAAATACAAAGATATGCCGTTCACTGTTGTGGGAGTCCATTCTGCAAAGTTTGATAATGAGAAGGATTTAGAAGCCATTCGCAATGCAGTGTTGCGCTATGGCATCACTCACCCAGTTGTCAATGATGGAGATATGTATCTATGGCGAGAACTAGGTATCAATTCTTGGCCAACATTTGCTATTGTTGGACCCAATGGTAGGCTCCTTGCACAACTGTCAGGTGAAGGTCGCCGCAAGGATCTTGACTGTTTGGTAGAGGCAGCTCTTTTGTTTTATGGCAGAAAGAAGATGTTGGACAATGCACCGATTCCTTTAAATTTGGAGAAAGATAATGATCCTCGTTTAGTTACATCCCCGCTAAAGTTTCCAGGAAAGCTTGCAATTGATGTTCTAAATGATAGGCTCTTCATTTCAGACAGTAATCATAACCGCATAGTGGTAACCGACCTAGATGGAAAATTTATTGTCCAAATAGGAAGTAGTGGAGAGGAAGGTTTGCGTGATGGTTCATTCGATGATGCCACCTTCAATCGGCCTCAGGGCCTAGCCTACAACCCAAGGAAAAACCTCCTCTATGTTGCAGATACTGAAAACCATGCTTTGAGGGAGATTGATTTTGTTAATGAGACAGTCCGTACTCTTGCTGGAAATGGAACCAAAGGCTCTGACTACCAAGGGGGAGGGAAAGGAACTACATTCTTTCAGCTCCTCAACTCACCATGGGACGTGTGCTTTCATCCAGTCAATGAGAAAGTTTATATTGCCATGGCTGGCTCACATCAAATCTGGGAACACAGTACAGATGATGGAGCTACTAGATCATTTAGTGGTGATGGTTACGAAAGAAACTTGAATGGATCAAGCTCTTCAAGCACATCGTTCGCACAGCCTTCTGGAATTTCATTATCTCTTGATCTCAGGGAGCTATATATTGCTGATAGTGAGAGTAGCTCCATTAGGGCACTTGATTTAAAAACAGGAGGGTCGAAATTGCTAGCTGGTGGTGATCCATATTTTGCGGAAAATTTGTTTAAGTTTGGTGACCACGATGGAATAGGGTCTGAAGTACTTCTTCAACACCCCCTAGGAGTCTTATGTGCAAAGAATGGTGAAGTTTATATAGCAGATAGTTACAATCACAAGATAAAGAAACTAGATCCAGCTGCTAAAAGAGTGAGTACCGTAGCAGGGACAGGAAAAGCTGGCTTCAAGGACGGAACATCTCTAGAAGCTCAGCTTTCAGAGCCATCAGGAATCGTTGAATCAAAAAACGGAAGAATTTTCGTAGCTGATACAAACAACAGTCTAATCAGATATCTAGACTTAAACAGGGAAGAAGCTGAACTTCTTACTTTGGAGCTGAAAGGGGTTCAGCCTCCCGTAGCAAAATCCAAGTCTTTGAAACGCCTCAGAAGGCGATCATCAGCTGACACGCAGACCATTACAGTTGATGGCGGCCCATCGAGCGAGGGCAACCTGTCTATTAAAATATCAGTACCTGAAGGGTATCATTTTTCAAAGGAAGCTCGCAGTAAGTTTAGCGTTGAAACTGATCCTGAAACTGCGGTTTCCATTGAGCCCGTTGATGGATATCTTAGTCCCGAAGGATCAGCAACTCTTCATTTCAAGAGAGCATCTCCCTCAGCTTCAATGGGGAGAATAAATTGCAAGGTTTACTATTGCAAGGAAGATGAGGTATGCTTATACCAATCGTTATTATTCGAAGTTCCATTTCGCGAGGAAATTCCAGGATCCAACCCAGAAGAAATAACACTGGCACACGTTGTAAAGCCGAAAACTTCAACAAGCAGCTTACAACTACCGGATGCGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 NHL Thioredoxin_8

Protein Analysis

1082

Amino Acids

117.85

Weight (kDa)

5.95

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 76 - 253 1.5e-22 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 78 - 260 1e-24 Haloacid dehalogenase-like hydrolase
AhpC-TSA PF00578 421 - 538 1e-06 AhpC/TSA family
Redoxin PF08534 431 - 541 1e-07 Redoxin
Thioredoxin_8 PF13905 443 - 537 1.9e-16 Thioredoxin-like
NHL PF01436 646 - 672 5.3e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 310, 661
AccB1I GGYRCC 1 cut(s) 491
AccB7I CCANNNNNTGG 1 cut(s) 473
AccI GTMKAC 1 cut(s) 294
AccII CGCG 2 cut(s) 3147, 3241
AciI CCGC 9 cut(s) 288, 747, 800, 1642, 1766, 1840, 2429, 2865, 2975
AcoI YGGCCR 3 cut(s) 3, 285, 1577
AcsI RAATTY 9 cut(s) 469, 961, 1265, 1726, 1868, 2307, 2434, 2688, 3153
AcuI CTGAAG 4 cut(s) 378, 827, 2493, 2931
AfaI GTAC 6 cut(s) 1383, 2047, 2209, 2478, 2590, 2907
AflIII ACRYGT 2 cut(s) 2136, 3190
AhlI ACTAGT 1 cut(s) 701
AjiI CACGTC 1 cut(s) 2137
AjnI CCWGG 4 cut(s) 487, 1230, 1777, 3157
AleI CACNNNNGTG 1 cut(s) 1436
AloI GAACNNNNNNTCC 2 cut(s) 1320, 1352
Alw26I GTCTC 3 cut(s) 103, 282, 2030
AlwNI CAGNNNCTG 2 cut(s) 1997, 2909
AoxI GGCC 9 cut(s) 3, 122, 285, 526, 744, 1577, 1943, 1951, 2866
ApoI RAATTY 9 cut(s) 469, 961, 1265, 1726, 1868, 2307, 2434, 2688, 3153
ArsI GACNNNNNNTTYG 2 cut(s) 2789, 2821
Asp700I GAANNNNTTC 6 cut(s) 80, 131, 431, 850, 1485, 2478
AspA2I CCTAGG 1 cut(s) 2494
AspLEI GCGC 3 cut(s) 1506, 3241, 3243
AspS9I GGNCC 6 cut(s) 818, 905, 1353, 1598, 1951, 2867
AsuHPI GGTGA 9 cut(s) 1110, 1193, 1511, 1643, 2118, 2252, 2426, 2462, 2531
AsuII TTCGAA 1 cut(s) 3132
AsuNHI GCTAGC 1 cut(s) 2404
AvaII GGWCC 4 cut(s) 818, 905, 1353, 1598
AvrII CCTAGG 1 cut(s) 2494
AxyI CCTNAGG 2 cut(s) 414, 1947
BaeGI GKGCMC 2 cut(s) 496, 2374
BalI TGGCCA 2 cut(s) 5, 1579
BamHI GGATCC 1 cut(s) 3161
BanI GGYRCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 2991
BbvCI CCTCAGC 1 cut(s) 3052
BceAI ACGGC 3 cut(s) 82, 259, 1411
BcgI CGANNNNNNTGC 2 cut(s) 2272, 2306
BciT130I CCWGG 4 cut(s) 489, 1232, 1779, 3159
BcoDI GTCTC 3 cut(s) 103, 282, 2030
BcuI ACTAGT 1 cut(s) 701
BfuAI ACCTGC 2 cut(s) 310, 661
BglII AGATCT 1 cut(s) 1190
BlnI CCTAGG 1 cut(s) 2494
BlpI GCTNAGC 2 cut(s) 1310, 2643
BmcAI AGTACT 1 cut(s) 2478
Bme1390I CCNGG 4 cut(s) 489, 1232, 1779, 3159
Bme18I GGWCC 4 cut(s) 818, 905, 1353, 1598
BmgBI CACGTC 1 cut(s) 2137
BmgT120I GGNCC 6 cut(s) 818, 905, 1353, 1598, 1951, 2867
BmiI GGNNCC 6 cut(s) 493, 906, 1600, 1613, 2065, 3163
BmrFI CCNGG 4 cut(s) 489, 1232, 1779, 3159
BmrI ACTGGG 1 cut(s) 1517
BmsI GCATC 9 cut(s) 554, 604, 622, 686, 715, 1521, 1918, 3053, 3226
BmtI GCTAGC 1 cut(s) 2408
BmuI ACTGGG 1 cut(s) 1517
BplI GAGNNNNNCTC 4 cut(s) 2345, 2377, 3031, 3063
BpmI CTGGAG 2 cut(s) 756, 1424
Bpu10I CCTNAGC 1 cut(s) 3052
Bpu1102I GCTNAGC 2 cut(s) 1310, 2643
Bpu14I TTCGAA 1 cut(s) 3132
BpuEI CTTGAG 2 cut(s) 894, 1107
BsaBI GATNNNNATC 3 cut(s) 1545, 1743, 3000
BsaJI CCNNGG 5 cut(s) 1967, 2083, 2129, 2175, 2494
BsaWI WCCGGW 1 cut(s) 3232
Bse1I ACTGG 6 cut(s) 115, 988, 1100, 1523, 2150, 3189
Bse21I CCTNAGG 2 cut(s) 414, 1947
Bse3DI GCAATG 2 cut(s) 1110, 1498
Bse8I GATNNNNATC 3 cut(s) 1545, 1743, 3000
BseBI CCWGG 4 cut(s) 489, 1232, 1779, 3159
BseDI CCNNGG 5 cut(s) 1967, 2083, 2129, 2175, 2494
BseGI GGATG 7 cut(s) 253, 677, 697, 821, 1760, 2146, 3241
BseJI GATNNNNATC 3 cut(s) 1545, 1743, 3000
BseMI GCAATG 2 cut(s) 1110, 1498
BseMII CTCAG 9 cut(s) 405, 524, 957, 1023, 1961, 2341, 2657, 2833, 3066
BseNI ACTGG 6 cut(s) 115, 988, 1100, 1523, 2150, 3189
BsePI GCGCGC 1 cut(s) 3239
BseRI GAGGAG 3 cut(s) 194, 1970, 2108
BseSI GKGCMC 2 cut(s) 496, 2374
BsgI GTGCAG 2 cut(s) 338, 761
Bsh1236I CGCG 2 cut(s) 3147, 3241
BshFI GGCC 9 cut(s) 5, 124, 287, 528, 746, 1579, 1945, 1953, 2868
BshNI GGYRCC 1 cut(s) 491
BsiSI CCGG 3 cut(s) 1271, 1388, 3233
BslFI GGGAC 6 cut(s) 680, 907, 918, 2147, 2614, 2994
BsmAI GTCTC 3 cut(s) 103, 282, 2030
BsmBI CGTCTC 1 cut(s) 282
BsmFI GGGAC 6 cut(s) 680, 907, 918, 2147, 2614, 2994
BsnI GGCC 9 cut(s) 5, 124, 287, 528, 746, 1579, 1945, 1953, 2868
Bsp119I TTCGAA 1 cut(s) 3132
Bsp1286I GDGCHC 3 cut(s) 496, 2374, 2991
Bsp1720I GCTNAGC 2 cut(s) 1310, 2643
Bsp19I CCATGG 2 cut(s) 2129, 2175
Bsp68I TCGCGA 1 cut(s) 3147
BspACI CCGC 9 cut(s) 288, 747, 800, 1642, 1766, 1840, 2429, 2865, 2975
BspANI GGCC 9 cut(s) 5, 124, 287, 528, 746, 1579, 1945, 1953, 2868
BspCNI CTCAG 9 cut(s) 406, 523, 958, 1022, 1960, 2340, 2656, 2832, 3065
BspFNI CGCG 2 cut(s) 3147, 3241
BspLI GGNNCC 6 cut(s) 493, 906, 1600, 1613, 2065, 3163
BspMI ACCTGC 2 cut(s) 310, 661
BspOI GCTAGC 1 cut(s) 2408
BspQI GCTCTTC 3 cut(s) 264, 1820, 2281
BspT104I TTCGAA 1 cut(s) 3132
BspT107I GGYRCC 1 cut(s) 491
BsrDI GCAATG 2 cut(s) 1110, 1498
BsrI ACTGG 6 cut(s) 115, 988, 1100, 1523, 2150, 3189
BssECI CCNNGG 5 cut(s) 1967, 2083, 2129, 2175, 2494
BssHII GCGCGC 1 cut(s) 3239
BssT1I CCWWGG 5 cut(s) 1967, 2083, 2129, 2175, 2494
Bst2UI CCWGG 4 cut(s) 489, 1232, 1779, 3159
Bst6I CTCTTC 6 cut(s) 116, 264, 422, 1820, 2281, 3033
BstAPI GCANNNNNTGC 2 cut(s) 551, 1384
BstBI TTCGAA 1 cut(s) 3132
BstC8I GCNNGC 7 cut(s) 744, 1788, 2182, 2406, 2614, 2938, 3241
BstDSI CCRYGG 2 cut(s) 2129, 2175
BstEII GGTNACC 2 cut(s) 1849, 2450
BstF5I GGATG 7 cut(s) 253, 677, 697, 821, 1760, 2146, 3241
BstFNI CGCG 2 cut(s) 3147, 3241
BstHHI GCGC 3 cut(s) 1506, 3241, 3243
BstMAI GTCTC 3 cut(s) 103, 282, 2030
BstMWI GCNNNNNNNGC 9 cut(s) 49, 551, 581, 752, 755, 1384, 2280, 2653, 2874
BstNI CCWGG 4 cut(s) 489, 1232, 1779, 3159
BstNSI RCATGY 1 cut(s) 1382
BstPI GGTNACC 2 cut(s) 1849, 2450
BstSCI CCNGG 4 cut(s) 487, 1230, 1777, 3157
BstSLI GKGCMC 2 cut(s) 496, 2374
BstUI CGCG 2 cut(s) 3147, 3241
BstX2I RGATCY 6 cut(s) 1190, 1318, 1390, 1648, 2566, 3161
BstXI CCANNNNNNTGG 1 cut(s) 688
BstYI RGATCY 6 cut(s) 1190, 1318, 1390, 1648, 2566, 3161
Bsu36I CCTNAGG 2 cut(s) 414, 1947
BsuRI GGCC 9 cut(s) 5, 124, 287, 528, 746, 1579, 1945, 1953, 2868
BtgI CCRYGG 2 cut(s) 2129, 2175
BtrI CACGTC 1 cut(s) 2137
BtsCI GGATG 7 cut(s) 253, 677, 697, 821, 1760, 2146, 3241
BtsI GCAGTG 1 cut(s) 1503
BtsIMutI CAGTG 5 cut(s) 122, 518, 1431, 1503, 3182
BtuMI TCGCGA 1 cut(s) 3147
BveI ACCTGC 2 cut(s) 310, 661
Cac8I GCNNGC 7 cut(s) 744, 1788, 2182, 2406, 2614, 2938, 3241
CaiI CAGNNNCTG 2 cut(s) 1997, 2909
CfoI GCGC 3 cut(s) 1506, 3241, 3243
Cfr13I GGNCC 6 cut(s) 818, 905, 1353, 1598, 1951, 2867
Csp6I GTAC 6 cut(s) 1382, 2046, 2208, 2477, 2589, 2906
CviAII CATG 7 cut(s) 7, 244, 349, 1379, 2006, 2130, 2176
CviQI GTAC 6 cut(s) 1382, 2046, 2208, 2477, 2589, 2906
DraI TTTAAA 2 cut(s) 1725, 2382
EaeI YGGCCR 3 cut(s) 3, 285, 1577
Eam1104I CTCTTC 6 cut(s) 116, 264, 422, 1820, 2281, 3033
EarI CTCTTC 6 cut(s) 116, 264, 422, 1820, 2281, 3033
EciI GGCGGA 1 cut(s) 815
Eco130I CCWWGG 5 cut(s) 1967, 2083, 2129, 2175, 2494
Eco147I AGGCCT 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 2991
Eco32I GATATC 3 cut(s) 664, 2726, 3002
Eco47I GGWCC 4 cut(s) 818, 905, 1353, 1598
Eco57I CTGAAG 4 cut(s) 378, 827, 2493, 2931
Eco81I CCTNAGG 2 cut(s) 414, 1947
Eco91I GGTNACC 2 cut(s) 1849, 2450
EcoO109I RGGNCCY 2 cut(s) 905, 1951
EcoO65I GGTNACC 2 cut(s) 1849, 2450
EcoRII CCWGG 4 cut(s) 487, 1230, 1777, 3157
EcoRV GATATC 3 cut(s) 664, 2726, 3002
EcoT14I CCWWGG 5 cut(s) 1967, 2083, 2129, 2175, 2494
EcoT22I ATGCAT 3 cut(s) 730, 773, 1380
EcoT38I GRGCYC 1 cut(s) 2991
ErhI CCWWGG 5 cut(s) 1967, 2083, 2129, 2175, 2494
Esp3I CGTCTC 1 cut(s) 282
FaeI CATG 7 cut(s) 10, 247, 352, 1382, 2009, 2133, 2179
FalI AAGNNNNNCTT 8 cut(s) 419, 451, 2466, 2498, 2631, 2663, 2739, 2771
FaqI GGGAC 6 cut(s) 680, 907, 918, 2147, 2614, 2994
FatI CATG 7 cut(s) 6, 243, 348, 1378, 2005, 2129, 2175
FauI CCCGC 1 cut(s) 1773
FblI GTMKAC 1 cut(s) 294
FokI GGATG 6 cut(s) 260, 664, 704, 808, 1747, 2133
FriOI GRGCYC 1 cut(s) 2991
GlaI GCGC 3 cut(s) 1505, 3240, 3242
GsuI CTGGAG 2 cut(s) 756, 1424
HaeIII GGCC 9 cut(s) 5, 124, 287, 528, 746, 1579, 1945, 1953, 2868
HapII CCGG 3 cut(s) 1271, 1388, 3233
HhaI GCGC 3 cut(s) 1506, 3241, 3243
Hin1II CATG 7 cut(s) 10, 247, 352, 1382, 2009, 2133, 2179
Hin6I GCGC 3 cut(s) 1504, 3239, 3241
HinP1I GCGC 3 cut(s) 1504, 3239, 3241
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 2 cut(s) 1051, 1784
HpaII CCGG 3 cut(s) 1271, 1388, 3233
HphI GGTGA 9 cut(s) 1110, 1193, 1511, 1643, 2118, 2252, 2426, 2462, 2531
Hpy166II GTNNAC 4 cut(s) 295, 1216, 1431, 3085
Hpy8I GTNNAC 4 cut(s) 295, 1216, 1431, 3085
Hpy99I CGWCG 1 cut(s) 299
HpyCH4IV ACGT 4 cut(s) 297, 1115, 2136, 3192
HpyF10VI GCNNNNNNNGC 9 cut(s) 49, 551, 581, 752, 755, 1384, 2280, 2653, 2874
HpySE526I ACGT 4 cut(s) 297, 1115, 2136, 3192
Hsp92II CATG 7 cut(s) 10, 247, 352, 1382, 2009, 2133, 2179
HspAI GCGC 3 cut(s) 1504, 3239, 3241
LguI GCTCTTC 3 cut(s) 264, 1820, 2281
LweI GCATC 9 cut(s) 554, 604, 622, 686, 715, 1521, 1918, 3053, 3226
MaeII ACGT 4 cut(s) 297, 1115, 2136, 3192
MaeIII GTNAC 8 cut(s) 322, 949, 1116, 1756, 1849, 2246, 2450, 2540
MfeI CAATTG 1 cut(s) 1791
MflI RGATCY 6 cut(s) 1190, 1318, 1390, 1648, 2566, 3161
MhlI GDGCHC 3 cut(s) 496, 2374, 2991
MlsI TGGCCA 2 cut(s) 5, 1579
MluNI TGGCCA 2 cut(s) 5, 1579
MlyI GAGTC 3 cut(s) 102, 1452, 2508
MmeI TCCRAC 4 cut(s) 1228, 1576, 1683, 3189
Mox20I TGGCCA 2 cut(s) 5, 1579
Mph1103I ATGCAT 3 cut(s) 730, 773, 1380
MroXI GAANNNNTTC 6 cut(s) 80, 131, 431, 850, 1485, 2478
MscI TGGCCA 2 cut(s) 5, 1579
MslI CAYNNNNRTG 1 cut(s) 1436
Msp20I TGGCCA 2 cut(s) 5, 1579
MspA1I CMGCKG 2 cut(s) 2573, 2837
MspI CCGG 3 cut(s) 1271, 1388, 3233
MspR9I CCNGG 4 cut(s) 489, 1232, 1779, 3159
MunI CAATTG 1 cut(s) 1791
MvaI CCWGG 4 cut(s) 489, 1232, 1779, 3159
MvnI CGCG 2 cut(s) 3147, 3241
MwoI GCNNNNNNNGC 9 cut(s) 49, 551, 581, 752, 755, 1384, 2280, 2653, 2874
NcoI CCATGG 2 cut(s) 2129, 2175
NheI GCTAGC 1 cut(s) 2404
NlaIII CATG 7 cut(s) 10, 247, 352, 1382, 2009, 2133, 2179
NlaIV GGNNCC 6 cut(s) 493, 906, 1600, 1613, 2065, 3163
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 4 cut(s) 322, 949, 1116, 2450
NruI TCGCGA 1 cut(s) 3147
NsiI ATGCAT 3 cut(s) 730, 773, 1380
NspI RCATGY 1 cut(s) 1382
NspV TTCGAA 1 cut(s) 3132
OliI CACNNNNGTG 1 cut(s) 1436
PauI GCGCGC 1 cut(s) 3239
PceI AGGCCT 1 cut(s) 528
PciSI GCTCTTC 3 cut(s) 264, 1820, 2281
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PdmI GAANNNNTTC 6 cut(s) 80, 131, 431, 850, 1485, 2478
PflMI CCANNNNNTGG 1 cut(s) 473
PfoI TCCNGGA 2 cut(s) 1777, 3157
PleI GAGTC 3 cut(s) 102, 1451, 2507
PpsI GAGTC 3 cut(s) 102, 1451, 2507
PpuMI RGGWCCY 1 cut(s) 905
Psp5II RGGWCCY 1 cut(s) 905
Psp6I CCWGG 4 cut(s) 487, 1230, 1777, 3157
PspEI GGTNACC 2 cut(s) 1849, 2450
PspGI CCWGG 4 cut(s) 487, 1230, 1777, 3157
PspN4I GGNNCC 6 cut(s) 493, 906, 1600, 1613, 2065, 3163
PspPI GGNCC 6 cut(s) 818, 905, 1353, 1598, 1951, 2867
PspPPI RGGWCCY 1 cut(s) 905
PstNI CAGNNNCTG 2 cut(s) 1997, 2909
PsuI RGATCY 6 cut(s) 1190, 1318, 1390, 1648, 2566, 3161
PteI GCGCGC 1 cut(s) 3239
PvuII CAGCTG 2 cut(s) 2573, 2837
RruI TCGCGA 1 cut(s) 3147
RsaI GTAC 6 cut(s) 1383, 2047, 2209, 2478, 2590, 2907
RsaNI GTAC 6 cut(s) 1382, 2046, 2208, 2477, 2589, 2906
RseI CAYNNNNRTG 1 cut(s) 1436
SalI GTCGAC 1 cut(s) 293
SapI GCTCTTC 3 cut(s) 264, 1820, 2281
Sau96I GGNCC 6 cut(s) 818, 905, 1353, 1598, 1951, 2867
ScaI AGTACT 1 cut(s) 2478
SchI GAGTC 3 cut(s) 102, 1452, 2508
ScrFI CCNGG 4 cut(s) 489, 1232, 1779, 3159
SduI GDGCHC 3 cut(s) 496, 2374, 2991
SfaNI GCATC 9 cut(s) 554, 604, 622, 686, 715, 1521, 1918, 3053, 3226
SfuI TTCGAA 1 cut(s) 3132
SinI GGWCC 4 cut(s) 818, 905, 1353, 1598
SmiMI CAYNNNNRTG 1 cut(s) 1436
SmlI CTYRAG 2 cut(s) 909, 1122
SmoI CTYRAG 2 cut(s) 909, 1122
SpeI ACTAGT 1 cut(s) 701
SseBI AGGCCT 1 cut(s) 528
SsiI CCGC 9 cut(s) 288, 747, 800, 1642, 1766, 1840, 2429, 2865, 2975
SspI AATATT 1 cut(s) 850
StuI AGGCCT 1 cut(s) 528
StyD4I CCNGG 4 cut(s) 487, 1230, 1777, 3157
StyI CCWWGG 5 cut(s) 1967, 2083, 2129, 2175, 2494
TaiI ACGT 4 cut(s) 300, 1118, 2139, 3195
TatI WGTACW 3 cut(s) 1381, 2207, 2476
TauI GCSGC 4 cut(s) 290, 749, 1644, 2868
TscAI CASTG 5 cut(s) 122, 518, 1438, 1503, 3189
TseFI GTSAC 4 cut(s) 322, 949, 1116, 2450
Tsp45I GTSAC 4 cut(s) 322, 949, 1116, 2450
TspDTI ATGAA 8 cut(s) 23, 337, 779, 1807, 1908, 2135, 2301, 3020
TspGWI ACGGA 4 cut(s) 376, 2033, 2640, 2697
TspRI CASTG 5 cut(s) 122, 518, 1438, 1503, 3189
Van91I CCANNNNNTGG 1 cut(s) 473
VpaK11BI GGWCC 4 cut(s) 818, 905, 1353, 1598
XapI RAATTY 9 cut(s) 469, 961, 1265, 1726, 1868, 2307, 2434, 2688, 3153
XbaI TCTAGA 3 cut(s) 1393, 2635, 2728
XceI RCATGY 1 cut(s) 1382
XmaJI CCTAGG 1 cut(s) 2494
XmiI GTMKAC 1 cut(s) 294
XmnI GAANNNNTTC 6 cut(s) 80, 131, 431, 850, 1485, 2478
ZrmI AGTACT 1 cut(s) 2478
Zsp2I ATGCAT 3 cut(s) 730, 773, 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.