Rroxscaffold_6G00422020

NHL repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008360
Physical Location & Seq
Reverse (-)
43044322 .. 43054526
10205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422020.1

Sequence Viewer

Length: 3231 bp
ATGGCCATGAAGCTACTCTCACCACCCTCCTCTTCTCTCTCACAACCCACCAGGCTCTTCTGCTCTCACCCCAACTCAACCAGACCAAAACCCATTTCAGTTCCAAACCTTCTCTTCCAAAGGACCCCAAAACGGTTCGTTTTCTCCAAGAGAATGGTAGTCAAAGCTTGTGTGAAAGTGGAGGAGAAGAACGTTCAAGAATCTTCTGGAAGTGAGTGGGGGAAGGTCTCTGCTGTGCTGTTTGACATGGATGGAGTTCTCTGCAATAGCGAAGAGCCTTCTAGAAGGGCCGCAGTCGAAGTTTTCGCGGAAATGGGGGTTGAGACCACGGTGGAGGACTTTGTTCCTTTCGGAGGAACTGGTGAAGCGAATTTTTTAGGAGGTGTTGCTGCTGTTAAGGGGGTAAAGGGATTCGATACCGAGGCAGCAAAGAAGAGATTCTTTGAGATATATTTGGATAAGTATGCAAAACCAGATTCAGGAATAGGATACCCCGGTGCCCTTGAACTTGTTACTCAGTGTAAAAGCAAAGGCCTTAAGGTTGCTGTTGCATCCAGTGCTGATCTAATCAAGGTTAAGGCAAATCTAGCTGCTGCCAATTTACCACTTTCATTGTTTGATGCTATTGTGTCAGCAGATGCTTTTGAGAATCTGAAACCTTCTCCTGATATATTCTTGGCTGCATCAAAGATCTTGGATGTAATCCCTAGTGAGTGTATTGTTATTGAGGATGCACTAGCTGGAGTGCAGGCTGCCAACGCTGCAAAAATGAGATGTATAGCTGTGAAAACTACTCTATCAGAAGAGGCTCTAATGAGTGCTGGCCCATCCATTATTCGAAATCAAATAGGAAATATTTCACTCGATGATATTCTCAGTGGTGGCTCTGATGGCTATAATGGGAAGATGCAGGGACTTCAATTTCTTAATTCTTCATCTCAAACCACATCAGAAAAGCTCACGGAAAGAGATACTGATTCTTCTGGTGCTGCCAATGACGGCATTTTCTCGGTTGTTCGGCGAGATATAGTGAAATATGGAAGCTTGGGGATTGCTTTGTCTTGTCTTGCCTTCACCATATCAAACTGGAAGGCAATGCAATATGCATCACCTAACGCTATTTGGAATGTGATATTTGGGGTCAACCAGCCATATATTGCACAGAAAGAAGGTGAATCAAAGTTGGAAAGAATCCAACAATTTGTGAACTATATATCTGACCTGGAAACCAGAGGAACTGCTCCTATTGTGCCAGAATTTCCACCAAAACTTGATTGGTTGAATACAGCTCCCATCAAGTTAAGCAAGGATCTCAAAGGAAAAGTGGTTGTGCTGGACTTTTGGACCTATTGCTGTATAAATTGTATGCATGTACTGCCAGACCTGGAGTTTTTGGAGAAAAAATACAAAGACATGCCGTTCACTGTGGTGGGAGTTCATTCGGCAAAATTTGATAATGAGAAGGATTTAGAGGCCATTCGGAATGCAGTGTTACGCTATAACATCACTCACCCAGTAGTCAATGATGGAGATATGTATCTATGGAGAGAGCTAGGTGTGAATTCATGGCCAACTTTTGCTGTTGTTGGGCCCAATGGAAGACTTCTTGCTCAACTATCAGGTGAAGGCCGCCGCAAGGATCTTGATGATTTGGTGGAGGCAGCTCTTTTGTACTATGGCCGAAAGAAAATCTTGGACAATGCACCAATTCCTTTGAGTTTGGAGAAAGATAATGATCCCCGTCTGTTTACATCTCCATTAAAGTTTCCTGGAAAGCTGGCGGTTGATGTCGAAAACAATAGGCTCTTCATTTCAGACAGTAATCATAACCGCATAGTTGTAACCGACCTAGATGGAAATTTTATTGTCCAAATTGGAAGTACAGGAGAGGAAGGTCTACGTGATGGTTCTTTTGATGATGCCACCTTTAATCGGCCTCAGGGCCTGGCTTACAATACAAAAAAAAATCTTCTCTACGTCGCGGATACTGAAAACCATGCGTTGAGGGAGATTGACTTTGTTAACGAAACAGTGCGTACTCTAGCTGGAAATGGAACCAAAGGCTCTGATTACAGAGGAGGAGGAAAAGGAAGCACTCAGCTACTCAACTCTCCATGGGATGTCTGCTTTCATCCATTCAATGAGAAAGTTTATATTGCCATGGCTGGCCAACATCAAATTTGGCAACTTGATACAGCTGATGGGATTACTAGAGCATTTAGTGGTGATGGGTATGAAAGAAATCTGAATGGATCAAGCTCTTCAAGCACATCATTTGCACAGCCTTCCGGAATTTCATTATCTCCAGATATGACGGAGCTGTATATTGCTGATAGTGAGAGTAGCTCCATCAGGGCAGTTGTTTTAAAAACAGGAGGATCGAGATTGCTAGCGGGTGGTGATCCAGTTTTCTCAGACAATTTGTTTAAGTTTGGGGACCATGATGGAATGGGTTCTGAAGTACTTCTTCAACATCCACTCGGTGTCTTGTGTACAAAGGGCGGTCAAATCTATATAGCAGATAGCTATAATCATAAGATAAAGAAGCTAGATCCAGCTAGTAAAAGAGTTAGTACCATAGCAGGGACAGGGAAAGCTGGTTTCAAGGACGGAACAGCTCTTGAAGCTCAGCTTTCAGAGCCATCAGGAATCATTGAAGCGAAAAATGGGAGACTCTTCATAGCCGATACAAACAACAGTCTAATCAGATATATTGACTTAAGCAACAAAGAACCTGAACTTCTTACTCTGGAGCTAAAAGGGGTTCAGCCTCCCACAGCAAAATCCAAGTCTTTGAAACGCCTCAGAAGGCGCTCATCAGCTGACACACAGACCGTTACAGTTGATGGTGGTTCATCCAATGAGGGGAACCTCTATATTAAAATATCTTTACCTGAAGAGTATCATTTTTCAAAGGAAGCTCGCAGTAAGTTTAGTGTTGAAACTGAGCCTGAAACTGCAGTTCTCGTTGATCCCTCAGATGGATATCTTAGTCCAGAAGGATCAGCAGTACTTCATTTTAAGAGGCCTTCTCCCTCAGCTTCTGTGGGGAGAGTTAATTGCACGGTTTACTATTGCAAAGAAGATGAGGTCTGTTTATACCAATCCTTATTATTTGAGGTACCATTCCATGAGGAAATTCCAGAATCCAACCCAGAAGAAATCACACTTGCCTACCTTGTAAAGCCCAGAACTTCAACAGACAGCTTACAGCTACCAGTTGCACGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 NHL Thioredoxin_8

Protein Analysis

1076

Amino Acids

117.41

Weight (kDa)

5.6

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 77 - 255 1.9e-24 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 79 - 262 3.8e-22 Haloacid dehalogenase-like hydrolase
Redoxin PF08534 429 - 544 8.8e-09 Redoxin
AhpC-TSA PF00578 430 - 535 1.8e-07 AhpC/TSA family
Thioredoxin_8 PF13905 440 - 534 1.2e-16 Thioredoxin-like
NHL PF01436 643 - 669 2.4e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 3121
AccB1I GGYRCC 2 cut(s) 497, 3121
AccI GTMKAC 1 cut(s) 1897
AccII CGCG 2 cut(s) 308, 1982
AccIII TCCGGA 1 cut(s) 2288
AciI CCGC 9 cut(s) 291, 308, 1630, 1633, 1781, 1831, 1982, 2393, 2502
AclI AACGTT 1 cut(s) 192
AclWI GGATC 9 cut(s) 1317, 1647, 1730, 2260, 2386, 2396, 2546, 2966, 3010
AcoI YGGCCR 4 cut(s) 3, 1568, 1678, 2167
AcsI RAATTY 8 cut(s) 370, 1256, 1448, 1561, 1858, 2178, 2292, 3138
AcuI CTGAAG 2 cut(s) 2478, 2916
AdeI CACNNNGTG 1 cut(s) 2483
AfaI GTAC 9 cut(s) 1374, 1673, 1882, 2038, 2463, 2494, 2575, 3012, 3123
AfiI CCNNNNNNNGG 8 cut(s) 132, 353, 479, 1636, 1932, 2583, 2865, 2981
AflII CTTAAG 2 cut(s) 536, 2719
AjnI CCWGG 5 cut(s) 50, 1221, 1383, 1768, 1944
AjuI GAANNNNNNNTTGG 2 cut(s) 79, 111
AleI CACNNNNGTG 1 cut(s) 1427
Alw26I GTCTC 3 cut(s) 232, 317, 2665
AlwI GGATC 9 cut(s) 1317, 1647, 1730, 2260, 2386, 2396, 2546, 2966, 3010
AlwNI CAGNNNCTG 2 cut(s) 1945, 3044
Aor13HI TCCGGA 1 cut(s) 2288
ApaI GGGCCC 1 cut(s) 1593
ApeKI GCWGC 9 cut(s) 389, 425, 590, 593, 680, 752, 761, 989, 1661
ApoI RAATTY 8 cut(s) 370, 1256, 1448, 1561, 1858, 2178, 2292, 3138
ArsI GACNNNNNNTTYG 2 cut(s) 2774, 2806
Asp700I GAANNNNTTC 4 cut(s) 437, 856, 2452, 2463
Asp718I GGTACC 1 cut(s) 3121
AspLEI GCGC 1 cut(s) 2814
AspS9I GGNCC 8 cut(s) 123, 288, 824, 1344, 1589, 1590, 1942, 2437
AsuC2I CCSGG 1 cut(s) 495
AsuII TTCGAA 1 cut(s) 838
AsuNHI GCTAGC 1 cut(s) 2389
AvaII GGWCC 3 cut(s) 123, 1344, 2437
AxyI CCTNAGG 1 cut(s) 1938
BaeGI GKGCMC 2 cut(s) 502, 1593
BaeI ACNNNNGTAYC 2 cut(s) 481, 514
BalI TGGCCA 3 cut(s) 5, 1570, 2169
BanI GGYRCC 2 cut(s) 497, 3121
BanII GRGCYC 1 cut(s) 1593
BbsI GAAGAC 1 cut(s) 1606
BbvCI CCTCAGC 1 cut(s) 3037
BbvI GCAGC 9 cut(s) 376, 437, 577, 580, 667, 739, 748, 976, 1673
BceAI ACGGC 2 cut(s) 1015, 1402
BciT130I CCWGG 5 cut(s) 52, 1223, 1385, 1770, 1946
BciVI GTATCC 2 cut(s) 482, 1978
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 3 cut(s) 232, 317, 2665
BfmI CTRYAG 1 cut(s) 2958
BfoI RGCGCY 1 cut(s) 2815
BfrI CTTAAG 2 cut(s) 536, 2719
BfuI GTATCC 2 cut(s) 482, 1978
BglII AGATCT 1 cut(s) 690
BlpI GCTNAGC 1 cut(s) 2628
BmcAI AGTACT 2 cut(s) 2463, 3012
Bme1390I CCNGG 6 cut(s) 52, 495, 1223, 1385, 1770, 1946
Bme18I GGWCC 3 cut(s) 123, 1344, 2437
BmgT120I GGNCC 8 cut(s) 123, 288, 824, 1344, 1589, 1590, 1942, 2437
BmiI GGNNCC 7 cut(s) 125, 499, 1591, 2056, 2438, 2870, 3123
BmrFI CCNGG 6 cut(s) 52, 495, 1223, 1385, 1770, 1946
BmrI ACTGGG 1 cut(s) 1508
BmsI GCATC 8 cut(s) 560, 610, 628, 692, 721, 897, 1115, 1909
BmtI GCTAGC 1 cut(s) 2393
BmuI ACTGGG 1 cut(s) 1508
BpiI GAAGAC 1 cut(s) 1606
BplI GAGNNNNNCTC 4 cut(s) 2330, 2362, 3016, 3048
BpmI CTGGAG 4 cut(s) 762, 1406, 2289, 2771
Bpu10I CCTNAGC 1 cut(s) 3037
Bpu1102I GCTNAGC 1 cut(s) 2628
Bpu14I TTCGAA 1 cut(s) 838
BpuMI CCSGG 1 cut(s) 495
BsaAI YACGTR 1 cut(s) 1901
BsaBI GATNNNNATC 3 cut(s) 1536, 1734, 2985
BsaI GGTCTC 2 cut(s) 232, 317
BsaJI CCNNGG 5 cut(s) 327, 420, 493, 2114, 2160
BsaWI WCCGGW 1 cut(s) 2288
Bsc4I CCNNNNNNNGG 8 cut(s) 132, 353, 479, 1636, 1932, 2583, 2865, 2981
Bse1I ACTGG 6 cut(s) 364, 555, 1091, 1514, 2405, 3218
Bse21I CCTNAGG 1 cut(s) 1938
Bse3DI GCAATG 1 cut(s) 1101
Bse8I GATNNNNATC 3 cut(s) 1536, 1734, 2985
BseAI TCCGGA 1 cut(s) 2288
BseBI CCWGG 5 cut(s) 52, 1223, 1385, 1770, 1946
BseDI CCNNGG 5 cut(s) 327, 420, 493, 2114, 2160
BseGI GGATG 9 cut(s) 256, 551, 703, 736, 827, 2125, 2131, 2473, 2855
BseJI GATNNNNATC 3 cut(s) 1536, 1734, 2985
BseLI CCNNNNNNNGG 8 cut(s) 132, 353, 479, 1636, 1932, 2583, 2865, 2981
BseMI GCAATG 1 cut(s) 1101
BseNI ACTGG 6 cut(s) 364, 555, 1091, 1514, 2405, 3218
BseRI GAGGAG 4 cut(s) 19, 197, 2091, 2094
BseSI GKGCMC 2 cut(s) 502, 1593
BseXI GCAGC 9 cut(s) 376, 437, 577, 580, 667, 739, 748, 976, 1673
BsgI GTGCAG 1 cut(s) 767
Bsh1236I CGCG 2 cut(s) 308, 1982
BshNI GGYRCC 2 cut(s) 497, 3121
BsiSI CCGG 2 cut(s) 495, 2289
BslFI GGGAC 3 cut(s) 927, 2450, 2599
BslI CCNNNNNNNGG 8 cut(s) 132, 353, 479, 1636, 1932, 2583, 2865, 2981
BsmAI GTCTC 3 cut(s) 232, 317, 2665
BsmFI GGGAC 3 cut(s) 927, 2450, 2599
BsmI GAATGC 1 cut(s) 1489
Bso31I GGTCTC 2 cut(s) 232, 317
Bsp119I TTCGAA 1 cut(s) 838
Bsp120I GGGCCC 1 cut(s) 1589
Bsp1286I GDGCHC 2 cut(s) 502, 1593
Bsp13I TCCGGA 1 cut(s) 2288
Bsp1407I TGTACA 1 cut(s) 2492
Bsp1720I GCTNAGC 1 cut(s) 2628
Bsp19I CCATGG 2 cut(s) 2114, 2160
BspACI CCGC 9 cut(s) 291, 308, 1630, 1633, 1781, 1831, 1982, 2393, 2502
BspEI TCCGGA 1 cut(s) 2288
BspFNI CGCG 2 cut(s) 308, 1982
BspLI GGNNCC 7 cut(s) 125, 499, 1591, 2056, 2438, 2870, 3123
BspMAI CTGCAG 1 cut(s) 2962
BspOI GCTAGC 1 cut(s) 2393
BspPI GGATC 9 cut(s) 1317, 1647, 1730, 2260, 2386, 2396, 2546, 2966, 3010
BspQI GCTCTTC 4 cut(s) 62, 267, 1811, 2266
BspT104I TTCGAA 1 cut(s) 838
BspT107I GGYRCC 2 cut(s) 497, 3121
BspTI CTTAAG 2 cut(s) 536, 2719
BspTNI GGTCTC 2 cut(s) 232, 317
BsrDI GCAATG 1 cut(s) 1101
BsrGI TGTACA 1 cut(s) 2492
BsrI ACTGG 6 cut(s) 364, 555, 1091, 1514, 2405, 3218
BssECI CCNNGG 5 cut(s) 327, 420, 493, 2114, 2160
BssT1I CCWWGG 2 cut(s) 2114, 2160
Bst2UI CCWGG 5 cut(s) 52, 1223, 1385, 1770, 1946
Bst4CI ACNGT 9 cut(s) 135, 331, 1426, 1820, 2032, 2699, 2836, 2842, 3067
BstAFI CTTAAG 2 cut(s) 536, 2719
BstAPI GCANNNNNTGC 2 cut(s) 557, 1375
BstAUI TGTACA 1 cut(s) 2492
BstBAI YACGTR 1 cut(s) 1901
BstBI TTCGAA 1 cut(s) 838
BstC8I GCNNGC 7 cut(s) 750, 823, 1779, 2167, 2391, 2923, 3226
BstDSI CCRYGG 3 cut(s) 327, 2114, 2160
BstENI CCTNNNNNAGG 1 cut(s) 351
BstF5I GGATG 9 cut(s) 256, 551, 703, 736, 827, 2125, 2131, 2473, 2855
BstFNI CGCG 2 cut(s) 308, 1982
BstH2I RGCGCY 1 cut(s) 2815
BstHHI GCGC 1 cut(s) 2814
BstMAI GTCTC 3 cut(s) 232, 317, 2665
BstMWI GCNNNNNNNGC 9 cut(s) 557, 587, 758, 761, 891, 1375, 2265, 2624, 2638
BstNI CCWGG 5 cut(s) 52, 1223, 1385, 1770, 1946
BstNSI RCATGY 2 cut(s) 1373, 1417
BstSCI CCNGG 6 cut(s) 50, 493, 1221, 1383, 1768, 1944
BstSFI CTRYAG 1 cut(s) 2958
BstSLI GKGCMC 2 cut(s) 502, 1593
BstUI CGCG 2 cut(s) 308, 1982
BstV1I GCAGC 9 cut(s) 376, 437, 577, 580, 667, 739, 748, 976, 1673
BstV2I GAAGAC 1 cut(s) 1606
BstX2I RGATCY 4 cut(s) 690, 1309, 1639, 2551
BstXI CCANNNNNNTGG 1 cut(s) 154
BstYI RGATCY 4 cut(s) 690, 1309, 1639, 2551
Bsu36I CCTNAGG 1 cut(s) 1938
BsuI GTATCC 2 cut(s) 482, 1978
BtgI CCRYGG 3 cut(s) 327, 2114, 2160
BtsCI GGATG 9 cut(s) 256, 551, 703, 736, 827, 2125, 2131, 2473, 2855
BtsI GCAGTG 1 cut(s) 1494
BtsIMutI CAGTG 6 cut(s) 524, 562, 883, 1422, 1494, 2037
Cac8I GCNNGC 7 cut(s) 750, 823, 1779, 2167, 2391, 2923, 3226
CaiI CAGNNNCTG 2 cut(s) 1945, 3044
CfoI GCGC 1 cut(s) 2814
Cfr13I GGNCC 8 cut(s) 123, 288, 824, 1344, 1589, 1590, 1942, 2437
Csp6I GTAC 9 cut(s) 1373, 1672, 1881, 2037, 2462, 2493, 2574, 3011, 3122
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviQI GTAC 9 cut(s) 1373, 1672, 1881, 2037, 2462, 2493, 2574, 3011, 3122
DraI TTTAAA 1 cut(s) 2367
DraIII CACNNNGTG 1 cut(s) 2483
EaeI YGGCCR 4 cut(s) 3, 1568, 1678, 2167
Eco130I CCWWGG 2 cut(s) 2114, 2160
Eco147I AGGCCT 2 cut(s) 534, 3028
Eco24I GRGCYC 1 cut(s) 1593
Eco31I GGTCTC 2 cut(s) 232, 317
Eco32I GATATC 1 cut(s) 2987
Eco47I GGWCC 3 cut(s) 123, 1344, 2437
Eco57I CTGAAG 2 cut(s) 2478, 2916
Eco81I CCTNAGG 1 cut(s) 1938
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoO109I RGGNCCY 2 cut(s) 123, 1942
EcoRI GAATTC 1 cut(s) 1561
EcoRII CCWGG 5 cut(s) 50, 1221, 1383, 1768, 1944
EcoRV GATATC 1 cut(s) 2987
EcoT14I CCWWGG 2 cut(s) 2114, 2160
EcoT22I ATGCAT 2 cut(s) 1108, 1371
EcoT38I GRGCYC 1 cut(s) 1593
ErhI CCWWGG 2 cut(s) 2114, 2160
FalI AAGNNNNNCTT 8 cut(s) 425, 457, 1676, 1708, 2451, 2483, 2616, 2648
FaqI GGGAC 3 cut(s) 927, 2450, 2599
FauI CCCGC 1 cut(s) 2386
FblI GTMKAC 1 cut(s) 1897
FokI GGATG 9 cut(s) 263, 538, 710, 743, 814, 2118, 2132, 2460, 2842
FriOI GRGCYC 1 cut(s) 1593
GlaI GCGC 1 cut(s) 2813
GsuI CTGGAG 4 cut(s) 762, 1406, 2289, 2771
HaeII RGCGCY 1 cut(s) 2815
HapII CCGG 2 cut(s) 495, 2289
HhaI GCGC 1 cut(s) 2814
Hin6I GCGC 1 cut(s) 2812
HinP1I GCGC 1 cut(s) 2812
HincII GTYRAC 2 cut(s) 1144, 2023
HindII GTYRAC 2 cut(s) 1144, 2023
HindIII AAGCTT 2 cut(s) 165, 1042
HpaI GTTAAC 1 cut(s) 2023
HpaII CCGG 2 cut(s) 495, 2289
Hpy166II GTNNAC 8 cut(s) 1144, 1207, 1422, 1749, 1898, 2023, 2493, 3070
Hpy8I GTNNAC 8 cut(s) 1144, 1207, 1422, 1749, 1898, 2023, 2493, 3070
Hpy99I CGWCG 1 cut(s) 1982
HpyCH4III ACNGT 9 cut(s) 135, 331, 1426, 1820, 2032, 2699, 2836, 2842, 3067
HpyCH4IV ACGT 3 cut(s) 192, 1900, 1977
HpyF10VI GCNNNNNNNGC 9 cut(s) 557, 587, 758, 761, 891, 1375, 2265, 2624, 2638
HpySE526I ACGT 3 cut(s) 192, 1900, 1977
HspAI GCGC 1 cut(s) 2812
Kpn2I TCCGGA 1 cut(s) 2288
KpnI GGTACC 1 cut(s) 3125
KspAI GTTAAC 1 cut(s) 2023
LguI GCTCTTC 4 cut(s) 62, 267, 1811, 2266
LmnI GCTCC 5 cut(s) 1246, 1294, 2317, 2351, 2752
Lsp1109I GCAGC 9 cut(s) 376, 437, 577, 580, 667, 739, 748, 976, 1673
LweI GCATC 8 cut(s) 560, 610, 628, 692, 721, 897, 1115, 1909
MaeII ACGT 3 cut(s) 192, 1900, 1977
MaeIII GTNAC 4 cut(s) 511, 1491, 1840, 2836
MflI RGATCY 4 cut(s) 690, 1309, 1639, 2551
MhlI GDGCHC 2 cut(s) 502, 1593
MlsI TGGCCA 3 cut(s) 5, 1570, 2169
MluNI TGGCCA 3 cut(s) 5, 1570, 2169
MlyI GAGTC 1 cut(s) 2667
MmeI TCCRAC 3 cut(s) 1164, 1219, 3174
Mox20I TGGCCA 3 cut(s) 5, 1570, 2169
Mph1103I ATGCAT 2 cut(s) 1108, 1371
MroI TCCGGA 1 cut(s) 2288
MroXI GAANNNNTTC 4 cut(s) 437, 856, 2452, 2463
MscI TGGCCA 3 cut(s) 5, 1570, 2169
MslI CAYNNNNRTG 1 cut(s) 1427
Msp20I TGGCCA 3 cut(s) 5, 1570, 2169
MspA1I CMGCKG 2 cut(s) 2198, 2822
MspCI CTTAAG 2 cut(s) 536, 2719
MspI CCGG 2 cut(s) 495, 2289
MspR9I CCNGG 6 cut(s) 52, 495, 1223, 1385, 1770, 1946
Mva1269I GAATGC 1 cut(s) 1489
MvaI CCWGG 5 cut(s) 52, 1223, 1385, 1770, 1946
MvnI CGCG 2 cut(s) 308, 1982
MwoI GCNNNNNNNGC 9 cut(s) 557, 587, 758, 761, 891, 1375, 2265, 2624, 2638
NciI CCSGG 1 cut(s) 495
NcoI CCATGG 2 cut(s) 2114, 2160
NheI GCTAGC 1 cut(s) 2389
NlaIV GGNNCC 7 cut(s) 125, 499, 1591, 2056, 2438, 2870, 3123
NsiI ATGCAT 2 cut(s) 1108, 1371
NspI RCATGY 2 cut(s) 1373, 1417
NspV TTCGAA 1 cut(s) 838
OliI CACNNNNGTG 1 cut(s) 1427
PceI AGGCCT 2 cut(s) 534, 3028
PciSI GCTCTTC 4 cut(s) 62, 267, 1811, 2266
PctI GAATGC 1 cut(s) 1489
PdmI GAANNNNTTC 4 cut(s) 437, 856, 2452, 2463
PfoI TCCNGGA 1 cut(s) 1768
PleI GAGTC 1 cut(s) 2667
PpsI GAGTC 1 cut(s) 2667
Ppu21I YACGTR 1 cut(s) 1901
PpuMI RGGWCCY 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 123
Psp6I CCWGG 5 cut(s) 50, 1221, 1383, 1768, 1944
PspGI CCWGG 5 cut(s) 50, 1221, 1383, 1768, 1944
PspN4I GGNNCC 7 cut(s) 125, 499, 1591, 2056, 2438, 2870, 3123
PspOMI GGGCCC 1 cut(s) 1589
PspPI GGNCC 8 cut(s) 123, 288, 824, 1344, 1589, 1590, 1942, 2437
PspPPI RGGWCCY 1 cut(s) 123
PstI CTGCAG 1 cut(s) 2962
PstNI CAGNNNCTG 2 cut(s) 1945, 3044
PsuI RGATCY 4 cut(s) 690, 1309, 1639, 2551
PvuII CAGCTG 2 cut(s) 2198, 2822
RsaI GTAC 9 cut(s) 1374, 1673, 1882, 2038, 2463, 2494, 2575, 3012, 3123
RsaNI GTAC 9 cut(s) 1373, 1672, 1881, 2037, 2462, 2493, 2574, 3011, 3122
RseI CAYNNNNRTG 1 cut(s) 1427
SapI GCTCTTC 4 cut(s) 62, 267, 1811, 2266
Sau96I GGNCC 8 cut(s) 123, 288, 824, 1344, 1589, 1590, 1942, 2437
ScaI AGTACT 2 cut(s) 2463, 3012
SchI GAGTC 1 cut(s) 2667
ScrFI CCNGG 6 cut(s) 52, 495, 1223, 1385, 1770, 1946
SduI GDGCHC 2 cut(s) 502, 1593
SfaNI GCATC 8 cut(s) 560, 610, 628, 692, 721, 897, 1115, 1909
SfcI CTRYAG 1 cut(s) 2958
SfuI TTCGAA 1 cut(s) 838
SinI GGWCC 3 cut(s) 123, 1344, 2437
SmiMI CAYNNNNRTG 1 cut(s) 1427
SmlI CTYRAG 2 cut(s) 536, 2719
SmoI CTYRAG 2 cut(s) 536, 2719
SseBI AGGCCT 2 cut(s) 534, 3028
SsiI CCGC 9 cut(s) 291, 308, 1630, 1633, 1781, 1831, 1982, 2393, 2502
SspI AATATT 1 cut(s) 856
StuI AGGCCT 2 cut(s) 534, 3028
StyD4I CCNGG 6 cut(s) 50, 493, 1221, 1383, 1768, 1944
StyI CCWWGG 2 cut(s) 2114, 2160
TaaI ACNGT 9 cut(s) 135, 331, 1426, 1820, 2032, 2699, 2836, 2842, 3067
TaiI ACGT 3 cut(s) 195, 1903, 1980
TaqI TCGA 6 cut(s) 297, 414, 838, 864, 1791, 2381
TatI WGTACW 6 cut(s) 1372, 1671, 1880, 2461, 2492, 3010
TauI GCSGC 3 cut(s) 293, 1632, 1635
TscAI CASTG 6 cut(s) 524, 562, 883, 1429, 1494, 2037
TseI GCWGC 9 cut(s) 389, 425, 590, 593, 680, 752, 761, 989, 1661
TspGWI ACGGA 3 cut(s) 977, 2330, 2625
TspRI CASTG 6 cut(s) 524, 562, 883, 1429, 1494, 2037
Vha464I CTTAAG 2 cut(s) 536, 2719
VpaK11BI GGWCC 3 cut(s) 123, 1344, 2437
XagI CCTNNNNNAGG 1 cut(s) 351
XapI RAATTY 8 cut(s) 370, 1256, 1448, 1561, 1858, 2178, 2292, 3138
XbaI TCTAGA 1 cut(s) 281
XceI RCATGY 2 cut(s) 1373, 1417
XcmI CCANNNNNNNNNTGG 1 cut(s) 1272
XmiI GTMKAC 1 cut(s) 1897
XmnI GAANNNNTTC 4 cut(s) 437, 856, 2452, 2463
ZrmI AGTACT 2 cut(s) 2463, 3012
Zsp2I ATGCAT 2 cut(s) 1108, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.