MD12G1206000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012318
Physical Location & Seq
Reverse (-)
28644187 .. 28648928
4742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1206000.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGATTTCCAGAAGAAGAGAATCCAACTACTGCTCTTCATAGTTGGCATCATCGTCCTCAGTATAACAGCCGAAAAATGTCGGCAACTGCTTGGGGAAGAGCGTGCATCTCAGAGTGGGAAGTTTACAGTCTTTGACTGCTTTGATATGAGTTCCGGAACACTAGCTTGTGCTGTGAAAGAGGGAGTGAAGCTGTATGTTAACAACATCAGAGCTGCTCATGTTGAGACGATAAAGCATAAAGCGATTGAAAGTGAATTGTCTGATGCATTGTCGCAGGGGATATCTGCTAAAGATGCAGCCAAACAAGCACAGGAAGTAGGAGCAAAGGCAGCGAAGCTGGCAACTCGGAAAGCCAAGCGGATAATAGGTCCTATCATCTCGTCCGGATGGGACTTCTTTGAAGCCATATACTATGGTGGTACAATTACAGAAGGGACCTTCAGGGGCACGGGAACCTTGTTTGGCACCTATGGAGGAGGCTTTGTAGGAGAGCAAAGGCTTGGGAGATTTGGTTACCTTGTGGGAAGTCAATTGGGCAGTTGGGCAGGAGGTAGGATTGGATTGATGGTATATGATGTGATTGATGGAGTGAACTACTTGCTCCAATTTGGTCTACCCGAAAACAGTGAAGTTCGTGAAACTTCTGCTTATGAAAGTTCTGAAGGTTCTGATGACATTAATGTTGATGAAGTCCCCACATATGAGAACCCTGAAGCTGAAGCCTACAAAACCTATGAGAGCTCCGAAGCATATGAACATTCTGAACTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

28.16

Weight (kDa)

5.1

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56423 AT1G56423
fragaria_vesca FvH4_6g07440
malus_domestica MD04G1193100.v1.1 MD12G1206000.v1.1
prunus_persica Prupe.6G313200_v2.0.a1
pyrus_communis pycom04g08470 pycom04g17090 pycom12g19390
rosa_chinensis RchiOBHm_Chr3g0457041
rosa_laevigata RLG00000025249
rosa_multiflora Rmu_sc0000488.1_g000003
rosa_roxburghii Rroxscaffold_6G00422240
rosa_rugosa Rorug03G0018700
rosa_samantha Rh3AG078500 Rh3BG080300 Rh3CG080300 Rh3DG082100
rosa_wichuraiana Rw3G006740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 467
AccI GTMKAC 1 cut(s) 616
AccIII TCCGGA 2 cut(s) 155, 386
AciI CCGC 1 cut(s) 361
AcuI CTGAAG 4 cut(s) 427, 684, 735, 741
AfaI GTAC 1 cut(s) 424
AgsI TTSAA 2 cut(s) 251, 404
AluBI AGCT 6 cut(s) 167, 193, 215, 340, 719, 744
AluI AGCT 6 cut(s) 167, 193, 215, 340, 719, 744
Alw21I GWGCWC 1 cut(s) 746
Alw26I GTCTC 1 cut(s) 221
Aor13HI TCCGGA 2 cut(s) 155, 386
ApeKI GCWGC 3 cut(s) 215, 299, 332
AseI ATTAAT 1 cut(s) 681
AspS9I GGNCC 2 cut(s) 371, 438
AvaII GGWCC 2 cut(s) 371, 438
BaeGI GKGCMC 1 cut(s) 452
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 746
Bbv12I GWGCWC 1 cut(s) 746
BbvI GCAGC 3 cut(s) 202, 311, 344
BccI CCATC 3 cut(s) 384, 562, 581
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 1 cut(s) 164
BisI GCNGC 3 cut(s) 216, 300, 333
BlsI GCNGC 3 cut(s) 217, 301, 334
Bme18I GGWCC 2 cut(s) 371, 438
BmgT120I GGNCC 2 cut(s) 371, 438
BmiI GGNNCC 3 cut(s) 439, 457, 469
BmsI GCATC 4 cut(s) 57, 116, 256, 286
BsaWI WCCGGW 2 cut(s) 155, 386
BsaXI ACNNNNNCTCC 4 cut(s) 471, 499, 501, 529
BseAI TCCGGA 2 cut(s) 155, 386
BseGI GGATG 1 cut(s) 395
BseMII CTCAG 3 cut(s) 73, 125, 761
BseRI GAGGAG 1 cut(s) 492
BseSI GKGCMC 1 cut(s) 452
BseXI GCAGC 3 cut(s) 202, 311, 344
BshNI GGYRCC 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 746
BsiSI CCGG 2 cut(s) 156, 387
BslFI GGGAC 3 cut(s) 407, 451, 680
BsmAI GTCTC 1 cut(s) 221
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 3 cut(s) 407, 451, 680
Bsp1286I GDGCHC 2 cut(s) 452, 746
Bsp13I TCCGGA 2 cut(s) 155, 386
BspACI CCGC 1 cut(s) 361
BspCNI CTCAG 3 cut(s) 72, 124, 762
BspEI TCCGGA 2 cut(s) 155, 386
BspLI GGNNCC 3 cut(s) 439, 457, 469
BspQI GCTCTTC 2 cut(s) 41, 93
BspT107I GGYRCC 1 cut(s) 467
Bst4CI ACNGT 2 cut(s) 130, 629
Bst6I CTCTTC 3 cut(s) 11, 41, 93
BstC8I GCNNGC 2 cut(s) 105, 342
BstDEI CTNAG 3 cut(s) 59, 111, 770
BstEII GGTNACC 1 cut(s) 515
BstF5I GGATG 1 cut(s) 395
BstMAI GTCTC 1 cut(s) 221
BstMWI GCNNNNNNNGC 4 cut(s) 296, 308, 332, 341
BstPI GGTNACC 1 cut(s) 515
BstSLI GKGCMC 1 cut(s) 452
BstV1I GCAGC 3 cut(s) 202, 311, 344
BtsCI GGATG 1 cut(s) 395
BtsIMutI CAGTG 1 cut(s) 634
Cac8I GCNNGC 2 cut(s) 105, 342
Cfr13I GGNCC 2 cut(s) 371, 438
Csp6I GTAC 1 cut(s) 423
CviAII CATG 1 cut(s) 221
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 3 cut(s) 59, 111, 770
Eam1104I CTCTTC 3 cut(s) 11, 41, 93
EarI CTCTTC 3 cut(s) 11, 41, 93
Ecl136II GAGCTC 1 cut(s) 744
Eco24I GRGCYC 1 cut(s) 746
Eco32I GATATC 1 cut(s) 285
Eco47I GGWCC 2 cut(s) 371, 438
Eco53kI GAGCTC 1 cut(s) 744
Eco57I CTGAAG 4 cut(s) 427, 684, 735, 741
Eco91I GGTNACC 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 744
EcoO109I RGGNCCY 2 cut(s) 371, 438
EcoO65I GGTNACC 1 cut(s) 515
EcoRV GATATC 1 cut(s) 285
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 746
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 1 cut(s) 224
FaqI GGGAC 3 cut(s) 407, 451, 680
FatI CATG 1 cut(s) 220
FauNDI CATATG 2 cut(s) 703, 754
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 3 cut(s) 216, 300, 333
FokI GGATG 1 cut(s) 402
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 3 cut(s) 216, 300, 333
FspBI CTAG 1 cut(s) 164
GluI GCNGC 3 cut(s) 216, 300, 333
HapII CCGG 2 cut(s) 156, 387
Hin1II CATG 1 cut(s) 224
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 1 cut(s) 21
HpaI GTTAAC 1 cut(s) 202
HpaII CCGG 2 cut(s) 156, 387
Hpy166II GTNNAC 4 cut(s) 126, 202, 595, 617
Hpy188I TCNGA 8 cut(s) 114, 212, 265, 351, 664, 673, 748, 766
Hpy188III TCNNGA 4 cut(s) 10, 156, 387, 638
Hpy8I GTNNAC 4 cut(s) 126, 202, 595, 617
HpyAV CCTTC 3 cut(s) 428, 451, 659
HpyCH4III ACNGT 2 cut(s) 130, 629
HpyCH4V TGCA 3 cut(s) 107, 269, 299
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 308, 332, 341
HpyF3I CTNAG 3 cut(s) 59, 111, 770
Hsp92II CATG 1 cut(s) 224
Kpn2I TCCGGA 2 cut(s) 155, 386
KspAI GTTAAC 1 cut(s) 202
LguI GCTCTTC 2 cut(s) 41, 93
LmnI GCTCC 3 cut(s) 323, 609, 749
LpnPI CCDG 9 cut(s) 23, 169, 263, 299, 326, 400, 430, 534, 726
Lsp1109I GCAGC 3 cut(s) 202, 311, 344
LweI GCATC 4 cut(s) 57, 116, 256, 286
MaeI CTAG 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 515
MboII GAAGA 4 cut(s) 25, 28, 28, 110
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 2 cut(s) 452, 746
MluCI AATT 4 cut(s) 257, 426, 533, 608
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 6 cut(s) 68, 175, 470, 473, 545, 765
Mph1103I ATGCAT 1 cut(s) 271
MroI TCCGGA 2 cut(s) 155, 386
MseI TTAA 2 cut(s) 201, 681
MspI CCGG 2 cut(s) 156, 387
MunI CAATTG 1 cut(s) 533
MwoI GCNNNNNNNGC 4 cut(s) 296, 308, 332, 341
NdeI CATATG 2 cut(s) 703, 754
NlaIII CATG 1 cut(s) 224
NlaIV GGNNCC 3 cut(s) 439, 457, 469
NsiI ATGCAT 1 cut(s) 271
PciSI GCTCTTC 2 cut(s) 41, 93
PfeI GAWTC 1 cut(s) 21
PkrI GCNGC 3 cut(s) 217, 301, 334
PpuMI RGGWCCY 2 cut(s) 371, 438
PshBI ATTAAT 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 746
Psp5II RGGWCCY 2 cut(s) 371, 438
PspEI GGTNACC 1 cut(s) 515
PspN4I GGNNCC 3 cut(s) 439, 457, 469
PspPI GGNCC 2 cut(s) 371, 438
PspPPI RGGWCCY 2 cut(s) 371, 438
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SacI GAGCTC 1 cut(s) 746
SapI GCTCTTC 2 cut(s) 41, 93
SaqAI TTAA 2 cut(s) 201, 681
SatI GCNGC 3 cut(s) 216, 300, 333
Sau96I GGNCC 2 cut(s) 371, 438
SduI GDGCHC 2 cut(s) 452, 746
SfaNI GCATC 4 cut(s) 57, 116, 256, 286
SinI GGWCC 2 cut(s) 371, 438
Sse9I AATT 4 cut(s) 257, 426, 533, 608
SsiI CCGC 1 cut(s) 361
SspMI CTAG 1 cut(s) 164
SstI GAGCTC 1 cut(s) 746
TaaI ACNGT 2 cut(s) 130, 629
TasI AATT 4 cut(s) 257, 426, 533, 608
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 2 cut(s) 201, 681
Tru9I TTAA 2 cut(s) 201, 681
TscAI CASTG 1 cut(s) 634
TseI GCWGC 3 cut(s) 215, 299, 332
TspDTI ATGAA 4 cut(s) 28, 669, 705, 771
TspRI CASTG 1 cut(s) 634
VpaK11BI GGWCC 2 cut(s) 371, 438
VspI ATTAAT 1 cut(s) 681
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 1 cut(s) 164
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.