RLG00000025249

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012318
Physical Location & Seq
Reverse (-)
43386846 .. 43388585
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025249

Sequence Viewer

Length: 927 bp
ATGGATTTCCAGATGAACAGGATCCAATTAGCAGCCTTCATTGCTGGCATCATCGTCCTCAGTATCACAGCTGAAAAATGTCGGCAGCTGGTTGGGGAAGAGCATTCGTCTCAGAGTGGGAAGTTTACAATTATGAACTGCTTTGATATGAGTTCTGGAACGCTAGCTTGTGGGGTGAAAGAGGGTGTGAAACTATATGCCAACAACATCAGATCTGCTCATGTCGAGGCAGCAAGGCATAAAGCAATCGAAAGTGCTTTGGCTGATGCATTGTCACAGGGGATGGGTTCTGAAGAAGCAGCCAAGCTAGCACAGAAAGCAGGAGCAAAGGCAGCAAAATTAGCAAAACGGCAAGCCAAGCGGATAATAGGTCCTATAATCTCGTCTGGATGGGACTTTTTCGAAGCTATATACTACGGTGGTGCAGTGACAGAAGGGATCGTCAGGGGCACTGGCACCTTGTTTGGCACCTATGCTGGAGGCTTTCTAGGAGACCAAAGGCTTGGGAGATTCGGTTATCTTGTGGGGAGTCAGTTGGGCAGTTGGGTAGGAGGTAGGATTGGATTGATGGTCTATGATGTGACTAGTGGAGTTAATTACATAATTAGATTTGGTATACCCGAAAGTAGTGAAGTTGGTGAAGCTCCTCCTTATGAAAGGTCTGATGACTCTTACGTTAGTGAAGCCCCTACATACACGAGCTGTGAAGATTCTTACATTAGTCAATTGGGCAGCTGGGTAAGAGGTAGGATTAAATTGATGGTCTGTGATGTGATTTTTGGTATACCCAAAAGTAGTGAAGTTGGTGAAGCTCCTCCTTATGAAAGTTCTGATGACTCTTACGTCAGTGAAGCTCCTGCATACGCGAGCTCTGAAGATTCTAACGTATATGAGTCTTCATACGAATCTTCCAATGAAGAGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

309

Amino Acids

33.07

Weight (kDa)

4.9

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56423 AT1G56423
fragaria_vesca FvH4_6g07440
malus_domestica MD04G1193100.v1.1 MD12G1206000.v1.1
prunus_persica Prupe.6G313200_v2.0.a1
pyrus_communis pycom04g08470 pycom04g17090 pycom12g19390
rosa_chinensis RchiOBHm_Chr3g0457041
rosa_laevigata RLG00000025249
rosa_multiflora Rmu_sc0000488.1_g000003
rosa_roxburghii Rroxscaffold_6G00422240
rosa_rugosa Rorug03G0018700
rosa_samantha Rh3AG078500 Rh3BG080300 Rh3CG080300 Rh3DG082100
rosa_wichuraiana Rw3G006740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 842
AccB1I GGYRCC 2 cut(s) 455, 467
AccI GTMKAC 2 cut(s) 616, 784
AccII CGCG 1 cut(s) 866
AciI CCGC 1 cut(s) 361
AclWI GGATC 3 cut(s) 16, 29, 446
AcuI CTGAAG 2 cut(s) 312, 894
AhlI ACTAGT 1 cut(s) 584
Alw21I GWGCWC 2 cut(s) 872, 924
Alw26I GTCTC 2 cut(s) 114, 486
AlwI GGATC 3 cut(s) 16, 29, 446
ApeKI GCWGC 6 cut(s) 32, 85, 230, 299, 332, 732
AspS9I GGNCC 1 cut(s) 371
AsuHPI GGTGA 3 cut(s) 187, 650, 818
AsuII TTCGAA 1 cut(s) 402
AsuNHI GCTAGC 2 cut(s) 163, 307
AvaII GGWCC 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 452
BamHI GGATCC 1 cut(s) 21
BanI GGYRCC 2 cut(s) 455, 467
BanII GRGCYC 2 cut(s) 872, 924
BauI CACGAG 1 cut(s) 697
BbsI GAAGAC 1 cut(s) 888
Bbv12I GWGCWC 2 cut(s) 872, 924
BbvI GCAGC 6 cut(s) 44, 97, 242, 311, 344, 744
BccI CCATC 4 cut(s) 277, 384, 562, 754
BceAI ACGGC 1 cut(s) 365
BcoDI GTCTC 2 cut(s) 114, 486
BcuI ACTAGT 1 cut(s) 584
BfaI CTAG 4 cut(s) 164, 308, 488, 585
BglII AGATCT 1 cut(s) 212
BisI GCNGC 6 cut(s) 33, 86, 231, 300, 333, 733
BlsI GCNGC 6 cut(s) 34, 87, 232, 301, 334, 734
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 3 cut(s) 23, 457, 469
BmsI GCATC 2 cut(s) 57, 256
BmtI GCTAGC 2 cut(s) 167, 311
BpiI GAAGAC 1 cut(s) 888
BpmI CTGGAG 1 cut(s) 498
Bpu14I TTCGAA 1 cut(s) 402
BsaI GGTCTC 1 cut(s) 486
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bse1I ACTGG 1 cut(s) 457
Bse3DI GCAATG 1 cut(s) 39
BseGI GGATG 2 cut(s) 288, 395
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 2 cut(s) 73, 125
BseNI ACTGG 1 cut(s) 457
BseRI GAGGAG 2 cut(s) 636, 804
BseSI GKGCMC 1 cut(s) 452
BseXI GCAGC 6 cut(s) 44, 97, 242, 311, 344, 744
BseYI CCCAGC 1 cut(s) 735
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 866
BshNI GGYRCC 2 cut(s) 455, 467
BsiHKAI GWGCWC 2 cut(s) 872, 924
BslFI GGGAC 1 cut(s) 407
BsmAI GTCTC 2 cut(s) 114, 486
BsmBI CGTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 407
BsmI GAATGC 1 cut(s) 103
Bso31I GGTCTC 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 402
Bsp1286I GDGCHC 3 cut(s) 452, 872, 924
Bsp143I GATC 3 cut(s) 21, 212, 438
BspACI CCGC 1 cut(s) 361
BspCNI CTCAG 2 cut(s) 72, 124
BspFNI CGCG 1 cut(s) 866
BspLI GGNNCC 3 cut(s) 23, 457, 469
BspOI GCTAGC 2 cut(s) 167, 311
BspPI GGATC 3 cut(s) 16, 29, 446
BspQI GCTCTTC 2 cut(s) 93, 912
BspT104I TTCGAA 1 cut(s) 402
BspT107I GGYRCC 2 cut(s) 455, 467
BspTNI GGTCTC 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 1 cut(s) 457
BssMI GATC 3 cut(s) 21, 212, 438
BssNAI GTATAC 2 cut(s) 617, 785
BssSI CACGAG 1 cut(s) 697
Bst1107I GTATAC 2 cut(s) 617, 785
Bst2BI CACGAG 1 cut(s) 697
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 2 cut(s) 93, 912
BstBI TTCGAA 1 cut(s) 402
BstC8I GCNNGC 5 cut(s) 46, 165, 309, 354, 868
BstDEI CTNAG 2 cut(s) 59, 111
BstF5I GGATG 2 cut(s) 288, 395
BstFNI CGCG 1 cut(s) 866
BstKTI GATC 3 cut(s) 24, 215, 441
BstMAI GTCTC 2 cut(s) 114, 486
BstMBI GATC 3 cut(s) 21, 212, 438
BstMWI GCNNNNNNNGC 6 cut(s) 41, 308, 317, 332, 341, 358
BstSLI GKGCMC 1 cut(s) 452
BstUI CGCG 1 cut(s) 866
BstV1I GCAGC 6 cut(s) 44, 97, 242, 311, 344, 744
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 2 cut(s) 21, 212
BstXI CCANNNNNNTGG 1 cut(s) 503
BstYI RGATCY 2 cut(s) 21, 212
BstZ17I GTATAC 2 cut(s) 617, 785
BtsCI GGATG 2 cut(s) 288, 395
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 3 cut(s) 432, 450, 853
Cac8I GCNNGC 5 cut(s) 46, 165, 309, 354, 868
Cfr13I GGNCC 1 cut(s) 371
CviAII CATG 1 cut(s) 221
DdeI CTNAG 2 cut(s) 59, 111
DpnI GATC 3 cut(s) 23, 214, 440
DpnII GATC 3 cut(s) 21, 212, 438
DrdI GACNNNNNNGTC 1 cut(s) 842
DseDI GACNNNNNNGTC 1 cut(s) 842
Eam1104I CTCTTC 2 cut(s) 93, 912
EarI CTCTTC 2 cut(s) 93, 912
Ecl136II GAGCTC 2 cut(s) 870, 922
Eco24I GRGCYC 2 cut(s) 872, 924
Eco31I GGTCTC 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 371
Eco53kI GAGCTC 2 cut(s) 870, 922
Eco57I CTGAAG 2 cut(s) 312, 894
EcoICRI GAGCTC 2 cut(s) 870, 922
EcoO109I RGGNCCY 1 cut(s) 371
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 2 cut(s) 872, 924
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 1 cut(s) 224
FaqI GGGAC 1 cut(s) 407
FatI CATG 1 cut(s) 220
FblI GTMKAC 2 cut(s) 616, 784
Fnu4HI GCNGC 6 cut(s) 33, 86, 231, 300, 333, 733
FokI GGATG 2 cut(s) 295, 402
FriOI GRGCYC 2 cut(s) 872, 924
Fsp4HI GCNGC 6 cut(s) 33, 86, 231, 300, 333, 733
FspBI CTAG 4 cut(s) 164, 308, 488, 585
GluI GCNGC 6 cut(s) 33, 86, 231, 300, 333, 733
GsaI CCCAGC 1 cut(s) 739
GsuI CTGGAG 1 cut(s) 498
Hin1II CATG 1 cut(s) 224
HinfI GANTC 8 cut(s) 510, 529, 668, 710, 836, 878, 893, 905
HphI GGTGA 3 cut(s) 187, 650, 818
Hpy166II GTNNAC 3 cut(s) 126, 617, 785
Hpy188I TCNGA 7 cut(s) 114, 212, 292, 664, 832, 874, 926
Hpy188III TCNNGA 3 cut(s) 10, 156, 387
Hpy8I GTNNAC 3 cut(s) 126, 617, 785
HpyAV CCTTC 2 cut(s) 46, 428
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4IV ACGT 3 cut(s) 675, 843, 885
HpyCH4V TGCA 3 cut(s) 269, 425, 860
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 308, 317, 332, 341, 358
HpyF3I CTNAG 2 cut(s) 59, 111
HpySE526I ACGT 3 cut(s) 675, 843, 885
Hsp92II CATG 1 cut(s) 224
Kzo9I GATC 3 cut(s) 21, 212, 438
LguI GCTCTTC 2 cut(s) 93, 912
LmnI GCTCC 4 cut(s) 323, 649, 817, 859
Lsp1109I GCAGC 6 cut(s) 44, 97, 242, 311, 344, 744
LweI GCATC 2 cut(s) 57, 256
MaeI CTAG 4 cut(s) 164, 308, 488, 585
MaeII ACGT 3 cut(s) 675, 843, 885
MaeIII GTNAC 3 cut(s) 273, 427, 580
MalI GATC 3 cut(s) 23, 214, 440
MboI GATC 3 cut(s) 21, 212, 438
MboII GAAGA 6 cut(s) 110, 305, 719, 887, 888, 900
MfeI CAATTG 1 cut(s) 725
MflI RGATCY 2 cut(s) 21, 212
MhlI GDGCHC 3 cut(s) 452, 872, 924
MluCI AATT 7 cut(s) 26, 129, 338, 595, 603, 725, 755
MlyI GAGTC 4 cut(s) 538, 662, 830, 902
MnlI CCTC 8 cut(s) 68, 175, 220, 473, 545, 657, 737, 825
Mph1103I ATGCAT 1 cut(s) 271
MseI TTAA 2 cut(s) 594, 753
MspA1I CMGCKG 3 cut(s) 71, 88, 735
MunI CAATTG 1 cut(s) 725
Mva1269I GAATGC 1 cut(s) 103
MvnI CGCG 1 cut(s) 866
MwoI GCNNNNNNNGC 6 cut(s) 41, 308, 317, 332, 341, 358
NdeII GATC 3 cut(s) 21, 212, 438
NheI GCTAGC 2 cut(s) 163, 307
NlaIII CATG 1 cut(s) 224
NlaIV GGNNCC 3 cut(s) 23, 457, 469
NmuCI GTSAC 3 cut(s) 273, 427, 580
NsiI ATGCAT 1 cut(s) 271
NspV TTCGAA 1 cut(s) 402
PciSI GCTCTTC 2 cut(s) 93, 912
PctI GAATGC 1 cut(s) 103
PfeI GAWTC 4 cut(s) 510, 710, 878, 905
PkrI GCNGC 6 cut(s) 34, 87, 232, 301, 334, 734
PleI GAGTC 4 cut(s) 537, 662, 830, 901
PpsI GAGTC 4 cut(s) 537, 662, 830, 901
PpuMI RGGWCCY 1 cut(s) 371
Psp124BI GAGCTC 2 cut(s) 872, 924
Psp5II RGGWCCY 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 735
PspN4I GGNNCC 3 cut(s) 23, 457, 469
PspPI GGNCC 1 cut(s) 371
PspPPI RGGWCCY 1 cut(s) 371
PsuI RGATCY 2 cut(s) 21, 212
PvuII CAGCTG 3 cut(s) 71, 88, 735
SacI GAGCTC 2 cut(s) 872, 924
SapI GCTCTTC 2 cut(s) 93, 912
SaqAI TTAA 2 cut(s) 594, 753
SatI GCNGC 6 cut(s) 33, 86, 231, 300, 333, 733
Sau3AI GATC 3 cut(s) 21, 212, 438
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 4 cut(s) 538, 662, 830, 902
SduI GDGCHC 3 cut(s) 452, 872, 924
SfaNI GCATC 2 cut(s) 57, 256
SfuI TTCGAA 1 cut(s) 402
SinI GGWCC 1 cut(s) 371
SpeI ACTAGT 1 cut(s) 584
Sse9I AATT 7 cut(s) 26, 129, 338, 595, 603, 725, 755
SsiI CCGC 1 cut(s) 361
SspMI CTAG 4 cut(s) 164, 308, 488, 585
SstI GAGCTC 2 cut(s) 872, 924
TaaI ACNGT 1 cut(s) 419
TaiI ACGT 3 cut(s) 678, 846, 888
TaqI TCGA 3 cut(s) 225, 249, 402
TasI AATT 7 cut(s) 26, 129, 338, 595, 603, 725, 755
TfiI GAWTC 4 cut(s) 510, 710, 878, 905
Tru1I TTAA 2 cut(s) 594, 753
Tru9I TTAA 2 cut(s) 594, 753
TscAI CASTG 3 cut(s) 432, 457, 853
TseFI GTSAC 3 cut(s) 273, 427, 580
TseI GCWGC 6 cut(s) 32, 85, 230, 299, 332, 732
Tsp45I GTSAC 3 cut(s) 273, 427, 580
TspDTI ATGAA 6 cut(s) 28, 29, 149, 669, 837, 888
TspRI CASTG 3 cut(s) 432, 457, 853
VpaK11BI GGWCC 1 cut(s) 371
XmiI GTMKAC 2 cut(s) 616, 784
XspI CTAG 4 cut(s) 164, 308, 488, 585
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.