Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007777

Physical Location & Seq

Genomic Coordinates Forward (+)

32974321 .. 32981117

Gene Structure 6797 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1263400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGGACGCGCTGAGGAAGCAGCTCGACCAGCTCATGGGGGCCAACCGAAACGGCGACGTTCGGGAGGTCAACCGCAAGTATTACGATCGCGATGTGTGTCGCCTCTACTTGGTCGGCCTCTGCCCCCACGAGCTTTTTCAGTTAACGAAAATGGATATGGGTCCGTGCCCTAATGTCCACTCTTTGCAACTCAGAAAAGAATACGAGGAAGCGAAAGCGAAAGGGACTGATAACTATGACAGAGACCTGGAGGATGTCATAGATAGGCTCATTGTTGAGTGTGATAGGAAAATTACTAGAGCTCTTAAGCGCCTCGAGGATGATGATGCCAAGGCTGCTATTGCCATCTCTGTCTCTGAAGTTACTCAGACACCAGAGATACTTGAGTTGTCAAAGCAGATCAAGGAGAAGTTGAAAGAAGTTGATCAATATGATCTTGAAGGCAAGACAGATCTTAAGATTCGGGCTTTGGAGATAGTAGAAGAACTCAGAGGCATTAGAGCAGATAAACAGTCCACGTTACTTTTGGATGCTTTCAATAAGGACCGGGCAGCTTTACCTCAACCCCTGCCAAACCCACCTCCTTTGGCACCCTTGCCTGTAGTTGTTCCTGATGCAAGAACACAGGAAATGATAAATGAAAAGTTGAAGAAGGCAGAGGACCTTGGTGAGCAAGGTATGGTTGATGAAGCACAAAAGGCATTGGAAGAGGCTGAAGCGCTGAAGAAGCTGCCTGCCAGACAAGAGCCTATCGTGGATTCCTCCAAGTACACTGCTGCTGATGTTCGTATTACTGATCAAAAGCTACGCGTTTGTGACATCTGTGGAGCATTTTTGAGTGTTTATGACAGTGACCGTCGTTTAGCTGATCACTTTGGCGGGAAGCTTCATTTAGGCTATATACAAATCCGTGAGAAGTTAGCAGAGCTGCAGGAAGAGAAAAACAAGAGTCGTAAGGTTGATAGGTCTGATGATAGGAGATCAAAGGAGCGAAGTAAGGAATGTGACAGGGAATCAAGTAGGGATAGAGAACGTGGAGCTAGCCGGGACCGAGGTAGGGATTATGATCGTAGGAGCAGAGATCGCGATAGGTACTATGATCGAGATCGTGGATATGATCGCGATCGTGATAGAGATTCAGATCGCTCCCACAATTATGATTCAAGAAGTCGCCGTAGGTCACGCTCACGATCGAGGGAACGTTCCAGGGATTATGATCGCCACAGGCGTTATGACCGGTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LUC7

Protein Analysis

415

Amino Acids

48.59

Weight (kDa)

8.48

Isoelectric Point (pI)

57.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LUC7	PF03194	2 - 169	6.1e-39	LUC7 N_terminus
LUC7	PF03194	208 - 325	2.1e-31	LUC7 N_terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007777)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03340 AT3G03340 AT3G03340 AT5G17440 AT5G17440
fragaria_vesca	FvH4_6g00250 FvH4_6g00250 FvH4_6g00250
malus_domestica	MD04G1246800.v1.1 MD12G1263400.v1.1
prunus_persica	Prupe.6G363000_v2.0.a1
pyrus_communis	pycom04g21750 pycom12g24040
rosa_chinensis	RchiOBHm_Chr3g0447621
rosa_laevigata	RLG00000025983
rosa_multiflora	Rmu_sc0003550.1_g000003
rosa_roxburghii	Rroxscaffold_6G00425830
rosa_rugosa	Rorug02G0603600 Rorug02G0603700
rosa_samantha	Rh3AG003800 Rh3BG003300 Rh3CG003600 Rh3DG003600
rosa_wichuraiana	Rw3G000270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	314
AccB1I	GGYRCC	1 cut(s)	589
AccB7I	CCANNNNNTGG	1 cut(s)	34
AccII	CGCG	5 cut(s)	8, 90, 810, 1086, 1122
AciI	CCGC	2 cut(s)	73, 879
AclI	AACGTT	1 cut(s)	1201
AcuI	CTGAAG	3 cut(s)	378, 735, 743
AfaI	GTAC	3 cut(s)	770, 1094, 1241
AfeI	AGCGCT	1 cut(s)	720
AfiI	CCNNNNNNNGG	3 cut(s)	34, 109, 1057
AflII	CTTAAG	2 cut(s)	305, 455
AflIII	ACRYGT	1 cut(s)	808
AgeI	ACCGGT	1 cut(s)	1236
AgsI	TTSAA	5 cut(s)	415, 440, 538, 649, 1164
AjnI	CCWGG	2 cut(s)	246, 1205
Alw21I	GWGCWC	1 cut(s)	304
Alw26I	GTCTC	2 cut(s)	237, 358
Ama87I	CYCGRG	1 cut(s)	314
Aor51HI	AGCGCT	1 cut(s)	720
AoxI	GGCC	2 cut(s)	39, 115
ApeKI	GCWGC	6 cut(s)	19, 335, 551, 730, 776, 928
AsiGI	ACCGGT	1 cut(s)	1236
AspLEI	GCGC	3 cut(s)	10, 312, 721
AspS9I	GGNCC	5 cut(s)	39, 161, 544, 661, 1048
AsuC2I	CCSGG	2 cut(s)	548, 1046
AsuHPI	GGTGA	1 cut(s)	680
AsuNHI	GCTAGC	1 cut(s)	1040
AvaI	CYCGRG	1 cut(s)	314
AvaII	GGWCC	4 cut(s)	161, 544, 661, 1048
BaeGI	GKGCMC	1 cut(s)	170
BaeI	ACNNNNGTAYC	2 cut(s)	371, 404
BanI	GGYRCC	1 cut(s)	589
BanII	GRGCYC	1 cut(s)	304
BauI	CACGAG	1 cut(s)	128
Bbv12I	GWGCWC	1 cut(s)	304
BbvCI	CCTCAGC	1 cut(s)	11
BbvI	GCAGC	6 cut(s)	31, 322, 563, 717, 763, 915
BccI	CCATC	1 cut(s)	353
BceAI	ACGGC	2 cut(s)	67, 1158
BciT130I	CCWGG	2 cut(s)	248, 1207
BclI	TGATCA	3 cut(s)	424, 796, 868
BcnI	CCSGG	2 cut(s)	548, 1046
BcoDI	GTCTC	2 cut(s)	237, 358
BfaI	CTAG	3 cut(s)	297, 1041, 1243
BfmI	CTRYAG	2 cut(s)	600, 929
BfoI	RGCGCY	2 cut(s)	313, 722
BfrI	CTTAAG	2 cut(s)	305, 455
BglII	AGATCT	1 cut(s)	451
BisI	GCNGC	6 cut(s)	20, 336, 552, 731, 777, 929
BlsI	GCNGC	6 cut(s)	21, 337, 553, 732, 778, 930
Bme1390I	CCNGG	4 cut(s)	248, 548, 1046, 1207
Bme18I	GGWCC	4 cut(s)	161, 544, 661, 1048
BmeT110I	CYCGRG	1 cut(s)	314
BmgT120I	GGNCC	5 cut(s)	39, 161, 544, 661, 1048
BmiI	GGNNCC	4 cut(s)	40, 162, 591, 1049
BmrFI	CCNGG	4 cut(s)	248, 548, 1046, 1207
BmsI	GCATC	3 cut(s)	316, 520, 604
BmtI	GCTAGC	1 cut(s)	1044
BpmI	CTGGAG	1 cut(s)	269
Bpu10I	CCTNAGC	1 cut(s)	11
BpuEI	CTTGAG	1 cut(s)	404
BpuMI	CCSGG	2 cut(s)	548, 1046
BsaBI	GATNNNNATC	5 cut(s)	1065, 1104, 1122, 1140, 1215
BsaI	GGTCTC	1 cut(s)	237
BsaJI	CCNNGG	4 cut(s)	330, 664, 1051, 1206
BsaWI	WCCGGW	1 cut(s)	1236
Bsc4I	CCNNNNNNNGG	3 cut(s)	34, 109, 1057
Bse118I	RCCGGY	1 cut(s)	1236
Bse8I	GATNNNNATC	5 cut(s)	1065, 1104, 1122, 1140, 1215
BseBI	CCWGG	2 cut(s)	248, 1207
BseDI	CCNNGG	4 cut(s)	330, 664, 1051, 1206
BseGI	GGATG	3 cut(s)	259, 325, 535
BseJI	GATNNNNATC	5 cut(s)	1065, 1104, 1122, 1140, 1215
BseLI	CCNNNNNNNGG	3 cut(s)	34, 109, 1057
BseMII	CTCAG	3 cut(s)	205, 380, 502
BseSI	GKGCMC	1 cut(s)	170
BseXI	GCAGC	6 cut(s)	31, 322, 563, 717, 763, 915
Bsh1236I	CGCG	5 cut(s)	8, 90, 810, 1086, 1122
Bsh1285I	CGRYCG	3 cut(s)	88, 1126, 1193
BshFI	GGCC	2 cut(s)	41, 117
BshNI	GGYRCC	1 cut(s)	589
BshTI	ACCGGT	1 cut(s)	1236
BsiEI	CGRYCG	3 cut(s)	88, 1126, 1193
BsiHKAI	GWGCWC	1 cut(s)	304
BsiHKCI	CYCGRG	1 cut(s)	314
BsiSI	CCGG	3 cut(s)	547, 1045, 1237
BslFI	GGGAC	2 cut(s)	238, 1061
BslI	CCNNNNNNNGG	3 cut(s)	34, 109, 1057
BsmAI	GTCTC	2 cut(s)	237, 358
BsmFI	GGGAC	2 cut(s)	238, 1061
BsnI	GGCC	2 cut(s)	41, 117
Bso31I	GGTCTC	1 cut(s)	237
BsoBI	CYCGRG	1 cut(s)	314
Bsp1286I	GDGCHC	2 cut(s)	170, 304
Bsp68I	TCGCGA	3 cut(s)	90, 1086, 1122
BspACI	CCGC	2 cut(s)	73, 879
BspANI	GGCC	2 cut(s)	41, 117
BspCNI	CTCAG	3 cut(s)	204, 379, 501
BspFNI	CGCG	5 cut(s)	8, 90, 810, 1086, 1122
BspLI	GGNNCC	4 cut(s)	40, 162, 591, 1049
BspMAI	CTGCAG	1 cut(s)	933
BspOI	GCTAGC	1 cut(s)	1044
BspT107I	GGYRCC	1 cut(s)	589
BspTI	CTTAAG	2 cut(s)	305, 455
BspTNI	GGTCTC	1 cut(s)	237
BsrFI	RCCGGY	1 cut(s)	1236
BssAI	RCCGGY	1 cut(s)	1236
BssECI	CCNNGG	4 cut(s)	330, 664, 1051, 1206
BssSI	CACGAG	1 cut(s)	128
BssT1I	CCWWGG	2 cut(s)	330, 664
Bst2BI	CACGAG	1 cut(s)	128
Bst2UI	CCWGG	2 cut(s)	248, 1207
Bst4CI	ACNGT	3 cut(s)	513, 851, 857
Bst6I	CTCTTC	2 cut(s)	702, 930
BstAFI	CTTAAG	2 cut(s)	305, 455
BstC8I	GCNNGC	2 cut(s)	735, 1042
BstDEI	CTNAG	4 cut(s)	11, 191, 366, 488
BstF5I	GGATG	3 cut(s)	259, 325, 535
BstFNI	CGCG	5 cut(s)	8, 90, 810, 1086, 1122
BstH2I	RGCGCY	2 cut(s)	313, 722
BstHHI	GCGC	3 cut(s)	10, 312, 721
BstMAI	GTCTC	2 cut(s)	237, 358
BstMCI	CGRYCG	3 cut(s)	88, 1126, 1193
BstMWI	GCNNNNNNNGC	7 cut(s)	16, 28, 335, 341, 698, 727, 1083
BstNI	CCWGG	2 cut(s)	248, 1207
BstSCI	CCNGG	4 cut(s)	246, 546, 1044, 1205
BstSFI	CTRYAG	2 cut(s)	600, 929
BstSLI	GKGCMC	1 cut(s)	170
BstUI	CGCG	5 cut(s)	8, 90, 810, 1086, 1122
BstV1I	GCAGC	6 cut(s)	31, 322, 563, 717, 763, 915
BstX2I	RGATCY	1 cut(s)	451
BstYI	RGATCY	1 cut(s)	451
BsuRI	GGCC	2 cut(s)	41, 117
BtgZI	GCGATG	1 cut(s)	105
BtsCI	GGATG	3 cut(s)	259, 325, 535
BtsI	GCAGTG	1 cut(s)	771
BtsIMutI	CAGTG	2 cut(s)	771, 856
BtuMI	TCGCGA	3 cut(s)	90, 1086, 1122
Cac8I	GCNNGC	2 cut(s)	735, 1042
CfoI	GCGC	3 cut(s)	10, 312, 721
Cfr10I	RCCGGY	1 cut(s)	1236
Cfr13I	GGNCC	5 cut(s)	39, 161, 544, 661, 1048
CseI	GACGC	1 cut(s)	14
Csp6I	GTAC	3 cut(s)	769, 1093, 1240
CspAI	ACCGGT	1 cut(s)	1236
CspCI	CAANNNNNGTGG	2 cut(s)	567, 602
CviAII	CATG	1 cut(s)	34
CviQI	GTAC	3 cut(s)	769, 1093, 1240
DdeI	CTNAG	4 cut(s)	11, 191, 366, 488
Eam1104I	CTCTTC	2 cut(s)	702, 930
EarI	CTCTTC	2 cut(s)	702, 930
Ecl136II	GAGCTC	1 cut(s)	302
Eco130I	CCWWGG	2 cut(s)	330, 664
Eco24I	GRGCYC	1 cut(s)	304
Eco31I	GGTCTC	1 cut(s)	237
Eco47I	GGWCC	4 cut(s)	161, 544, 661, 1048
Eco47III	AGCGCT	1 cut(s)	720
Eco53kI	GAGCTC	1 cut(s)	302
Eco57I	CTGAAG	3 cut(s)	378, 735, 743
Eco88I	CYCGRG	1 cut(s)	314
EcoICRI	GAGCTC	1 cut(s)	302
EcoO109I	RGGNCCY	1 cut(s)	661
EcoRII	CCWGG	2 cut(s)	246, 1205
EcoT14I	CCWWGG	2 cut(s)	330, 664
EcoT38I	GRGCYC	1 cut(s)	304
ErhI	CCWWGG	2 cut(s)	330, 664
FaeI	CATG	1 cut(s)	37
FaqI	GGGAC	2 cut(s)	238, 1061
FatI	CATG	1 cut(s)	33
FauI	CCCGC	1 cut(s)	872
FbaI	TGATCA	3 cut(s)	424, 796, 868
Fnu4HI	GCNGC	6 cut(s)	20, 336, 552, 731, 777, 929
FokI	GGATG	3 cut(s)	266, 332, 542
FriOI	GRGCYC	1 cut(s)	304
Fsp4HI	GCNGC	6 cut(s)	20, 336, 552, 731, 777, 929
FspBI	CTAG	3 cut(s)	297, 1041, 1243
GlaI	GCGC	3 cut(s)	9, 311, 720
GluI	GCNGC	6 cut(s)	20, 336, 552, 731, 777, 929
GsuI	CTGGAG	1 cut(s)	269
HaeII	RGCGCY	2 cut(s)	313, 722
HaeIII	GGCC	2 cut(s)	41, 117
HapII	CCGG	3 cut(s)	547, 1045, 1237
HgaI	GACGC	1 cut(s)	14
HhaI	GCGC	3 cut(s)	10, 312, 721
Hin1II	CATG	1 cut(s)	37
Hin6I	GCGC	3 cut(s)	8, 310, 719
HinP1I	GCGC	3 cut(s)	8, 310, 719
HincII	GTYRAC	2 cut(s)	70, 144
HindII	GTYRAC	2 cut(s)	70, 144
HindIII	AAGCTT	1 cut(s)	884
HinfI	GANTC	6 cut(s)	460, 758, 949, 1013, 1136, 1160
HpaI	GTTAAC	1 cut(s)	144
HpaII	CCGG	3 cut(s)	547, 1045, 1237
HphI	GGTGA	1 cut(s)	680
Hpy166II	GTNNAC	5 cut(s)	70, 144, 178, 516, 771
Hpy188I	TCNGA	6 cut(s)	194, 358, 369, 491, 970, 1141
Hpy8I	GTNNAC	5 cut(s)	70, 144, 178, 516, 771
Hpy99I	CGWCG	2 cut(s)	59, 861
HpyAV	CCTTC	2 cut(s)	434, 646
HpyCH4III	ACNGT	3 cut(s)	513, 851, 857
HpyCH4IV	ACGT	4 cut(s)	57, 518, 1033, 1201
HpyCH4V	TGCA	3 cut(s)	187, 617, 931
HpyF10VI	GCNNNNNNNGC	7 cut(s)	16, 28, 335, 341, 698, 727, 1083
HpyF3I	CTNAG	4 cut(s)	11, 191, 366, 488
HpySE526I	ACGT	4 cut(s)	57, 518, 1033, 1201
Hsp92II	CATG	1 cut(s)	37
HspAI	GCGC	3 cut(s)	8, 310, 719
Ksp22I	TGATCA	3 cut(s)	424, 796, 868
KspAI	GTTAAC	1 cut(s)	144
LmnI	GCTCC	5 cut(s)	827, 988, 1037, 1074, 1151
Lsp1109I	GCAGC	6 cut(s)	31, 322, 563, 717, 763, 915
LweI	GCATC	3 cut(s)	316, 520, 604
MaeI	CTAG	3 cut(s)	297, 1041, 1243
MaeII	ACGT	4 cut(s)	57, 518, 1033, 1201
MaeIII	GTNAC	6 cut(s)	361, 519, 815, 851, 1004, 1179
MboII	GAAGA	5 cut(s)	494, 661, 719, 736, 947
MflI	RGATCY	1 cut(s)	451
MhlI	GDGCHC	2 cut(s)	170, 304
MluCI	AATT	2 cut(s)	291, 1153
MluI	ACGCGT	1 cut(s)	808
MlyI	GAGTC	1 cut(s)	958
MseI	TTAA	3 cut(s)	143, 306, 456
MslI	CAYNNNNRTG	1 cut(s)	1155
MspCI	CTTAAG	2 cut(s)	305, 455
MspI	CCGG	3 cut(s)	547, 1045, 1237
MspR9I	CCNGG	4 cut(s)	248, 548, 1046, 1207
MvaI	CCWGG	2 cut(s)	248, 1207
MvnI	CGCG	5 cut(s)	8, 90, 810, 1086, 1122
MwoI	GCNNNNNNNGC	7 cut(s)	16, 28, 335, 341, 698, 727, 1083
NciI	CCSGG	2 cut(s)	548, 1046
NheI	GCTAGC	1 cut(s)	1040
NlaIII	CATG	1 cut(s)	37
NlaIV	GGNNCC	4 cut(s)	40, 162, 591, 1049
NmuCI	GTSAC	4 cut(s)	815, 851, 1004, 1179
NruI	TCGCGA	3 cut(s)	90, 1086, 1122
PaeR7I	CTCGAG	1 cut(s)	314
PcsI	WCGNNNNNNNCGW	1 cut(s)	1225
PfeI	GAWTC	5 cut(s)	460, 758, 1013, 1136, 1160
PflMI	CCANNNNNTGG	1 cut(s)	34
PinAI	ACCGGT	1 cut(s)	1236
PkrI	GCNGC	6 cut(s)	21, 337, 553, 732, 778, 930
Ple19I	CGATCG	3 cut(s)	88, 1126, 1193
PleI	GAGTC	1 cut(s)	957
PpsI	GAGTC	1 cut(s)	957
PpuMI	RGGWCCY	1 cut(s)	661
Psp124BI	GAGCTC	1 cut(s)	304
Psp1406I	AACGTT	1 cut(s)	1201
Psp5II	RGGWCCY	1 cut(s)	661
Psp6I	CCWGG	2 cut(s)	246, 1205
PspGI	CCWGG	2 cut(s)	246, 1205
PspN4I	GGNNCC	4 cut(s)	40, 162, 591, 1049
PspPI	GGNCC	5 cut(s)	39, 161, 544, 661, 1048
PspPPI	RGGWCCY	1 cut(s)	661
PspXI	VCTCGAGB	1 cut(s)	314
PstI	CTGCAG	1 cut(s)	933
PsuI	RGATCY	1 cut(s)	451
PvuI	CGATCG	3 cut(s)	88, 1126, 1193
RruI	TCGCGA	3 cut(s)	90, 1086, 1122
RsaI	GTAC	3 cut(s)	770, 1094, 1241
RsaNI	GTAC	3 cut(s)	769, 1093, 1240
RseI	CAYNNNNRTG	1 cut(s)	1155
SacI	GAGCTC	1 cut(s)	304
SaqAI	TTAA	3 cut(s)	143, 306, 456
SatI	GCNGC	6 cut(s)	20, 336, 552, 731, 777, 929
Sau96I	GGNCC	5 cut(s)	39, 161, 544, 661, 1048
SchI	GAGTC	1 cut(s)	958
ScrFI	CCNGG	4 cut(s)	248, 548, 1046, 1207
SduI	GDGCHC	2 cut(s)	170, 304
SfaNI	GCATC	3 cut(s)	316, 520, 604
SfcI	CTRYAG	2 cut(s)	600, 929
Sfr274I	CTCGAG	1 cut(s)	314
SinI	GGWCC	4 cut(s)	161, 544, 661, 1048
SlaI	CTCGAG	1 cut(s)	314
SmiMI	CAYNNNNRTG	1 cut(s)	1155
SmlI	CTYRAG	4 cut(s)	305, 314, 383, 455
SmoI	CTYRAG	4 cut(s)	305, 314, 383, 455
Sse9I	AATT	2 cut(s)	291, 1153
SsiI	CCGC	2 cut(s)	73, 879
SspMI	CTAG	3 cut(s)	297, 1041, 1243
SstI	GAGCTC	1 cut(s)	304
StyD4I	CCNGG	4 cut(s)	246, 546, 1044, 1205
StyI	CCWWGG	2 cut(s)	330, 664
TaaI	ACNGT	3 cut(s)	513, 851, 857
TaiI	ACGT	4 cut(s)	60, 521, 1036, 1204
TaqI	TCGA	4 cut(s)	24, 315, 1102, 1193
TaqII	GACCGA	1 cut(s)	1065
TasI	AATT	2 cut(s)	291, 1153
TatI	WGTACW	1 cut(s)	768
TfiI	GAWTC	5 cut(s)	460, 758, 1013, 1136, 1160
Tru1I	TTAA	3 cut(s)	143, 306, 456
Tru9I	TTAA	3 cut(s)	143, 306, 456
TscAI	CASTG	2 cut(s)	778, 856
TseFI	GTSAC	4 cut(s)	815, 851, 1004, 1179
TseI	GCWGC	6 cut(s)	19, 335, 551, 730, 776, 928
Tsp45I	GTSAC	4 cut(s)	815, 851, 1004, 1179
TspDTI	ATGAA	3 cut(s)	654, 702, 878
TspGWI	ACGGA	2 cut(s)	153, 899
TspRI	CASTG	2 cut(s)	778, 856
Van91I	CCANNNNNTGG	1 cut(s)	34
Vha464I	CTTAAG	2 cut(s)	305, 455
VpaK11BI	GGWCC	4 cut(s)	161, 544, 661, 1048
XcmI	CCANNNNNNNNNTGG	1 cut(s)	523
XhoI	CTCGAG	1 cut(s)	314
XspI	CTAG	3 cut(s)	297, 1041, 1243

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.