MD13G1003300.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005277
Physical Location & Seq
Reverse (-)
210349 .. 220393
10045 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1003300.v1.1.491

Sequence Viewer

Length: 3525 bp
ATGTCGCAGAAAACCAGGGCCCCACCTTCCTTCCAGTCAATCAAATCATTGCCCGGAGATTTTAGGTTCTCAGGTCTACCAGCTTCTGATCGATTTGGAAAATCTGATGATGGGAATTTGGGAAATAGCAATGTCATCTCCTCGAGTATTCCAGAAAATGGTGGTTTTGAGGGAGTTGAGGGTAGTATTGGGCCTCTTGGCGACATGGAACAGGTAGACGATTCACCATATGGCAGGAACACAATCTCCATCGAAGACAGCCCCCCAAGGGGTGATGAAGACTTGGATTCTGTTGCTCCATCTATGCCTTCAATAGCTTCATCCCGTAGGGAACATAGGTGGGGTGACACCAATCCTTATGCTGTGAAGAAGCTTCAATCTTGGTTCCAACTTCCAAACCGTAATTGGGAACTGGGGAGAATATTGTCTACCTCAGGCGCTGAATCTGTTATATCACTTCCTAACGATAAAGTTGTAACGGTAAAAACTGAAGATTTGGTTTCAGCTAATCCAGATATCCTTGATGGTGTGGATGATCTCATGCAACTTAGTTACCTAAATGAGCCATCAGTGTTGTACAATCTTCAGTATAGATACAATCAGGATATGATTTATACAAAAGCGGGACCGGTGTTGGTTGCTGTCAATCCCTTTAAGAGAGTTTCTTTATATGGGAATGAATACATTGAAGCATACAAGCGTAAAGCAGTTGAAAGTCCACATGTGTATGCCATTGCAGACACAGCCATTCGGGAAATGGTTAGAGATGAAGTAAATCAGTCTATAATTATAAGTGGGGAAAGTGGAGCAGGAAAAACTGAAACTGCGAAGATAGCAATGCAATATTTGGCTGCACTTGGAGGTGGAAGTGGAATAGAGCATGAAATTTTGAAAACTAACCCGATACTGGAAGCCTTTGGTAATGCGAAAACATTGAGAAATGACAACTCAAGTCGCTTTGGAAAATTGATTGAAATCCACTTTAGTGAAACTGGAAAAATTTCTGGTGCCAATATTCAAACTTTTTTGCTTGAAAAGTCTAGAGTGGTCCAATGCACTGAAGGTGAAAGGTCCTATCATATATTTTATCAGCTTTGTGCTGGAGCTCCACCTGCTCTTAGAGAGATGCTGAACTTGAAGAGTGCAGATGAGTACCAATATCTGAAGCAAAGCAATTGTTATTCCATTTCTGGGGTTAATGATGCTGAAGAATTCAGTGTGGTGAAGGAAGCTCTAGATGTTGTCCATATAAACAAAGAAGACCAGCAAGGTGTATTTGCAATGCTTGCTGCAGTTTTGTGGCTGGGAAACATCTCATTTAGTGTGATTGATAATGAAAATCATGTTGAACCTGTAGAAGATGAAGGTTTGTTCAATGTCGCAAAATTGATTGGATGTGGTGTAGATGAGCTTAAGCTTGCTTTATCAACTCGAAATATGAGAGTTGGTATTGACAGTATTGTCCAAAAGCTAACGCTATCACAGGCCATCGACACAAGAGATGCTTTGGCAAAGTCAATTTATGCATGTCTGTTTGAGTGGCTGGTTGAACAAATTAACAAATCACTTGCAGTAGGAAAACGGCGTACTGGCAGATCTATCAGCATTCTTGATATCTATGGTTTTGAATCATTTGATAGAAATAGTTTTGAACAGTTCTGCATTAATTATGCAAATGAGAGATTGCAACAACACTTCAATCGTCACTTATTCAAGTTAGAGCAGGATGAATACATCCAAGATGGAATTGATTGGACAAAAGTTGAATTTGAAGACAACCAAGATTGTCTTGGTCTTTTTGAAAAGAGGCCGTTGGGATTACTGTCCCTGTTAGATGAGGAGTCCACCTTCCCAAATGGTACTGATTTGACCTTTGCCAACAAGCTTAAGCAACATCTGAGTTCTAATTCTTGTTTCAAAGGAGAACGAGACAAATCGTTTGCTGTCAGTCATTATGCAGGAGAGGTTTCATATGACACAACAGGATTTTTGGAGAAGAACAGGGACTTACTGCATTTAGATTCTATTCAGCTTCTTTCATCATGCTCATGCCACCTTCCTCAGATATTCGCATCTAGTATGCTTAATCGACCTGAGAAGCCTTTAGTTGGTCCCCTGTATAAAACAGGTGGCGGAGTAGACTCCCAGAAGCTGAGTGTTGCAACGAAATTTAAGAGTCAATTGTTCCTATTGATGAAACGTCTAGAAAACACCACTCCACATTTCATACGTTGTATCAAGCCCAACAATCTTCAATCCCCTGGACTATATGAGCAAGGACTCGTACTGCAGCAGCTGAGATGTTGTGGAGTTCTAGAAGTGGTTCGCATATCAAGATCTGGCTTTCCTACCAGAATGTCACACCAGAAGTTTGCAAGAAGATATGGTTTTCTTCTGCTGGAGAATGTTGCATCGCAGGATCCACTTAGTGTTTCAGTAGCTATTCTTCACCAGTTCAACATATTACCAGAGATGTATCAAGTGGGCTATACAAAGTTGTTTTTCCGAACTGGACAGATTGGGGTGCTTGAGGACACCAGAAACCGTACGCTCCATGGTATTTTACGTGTTCAAAGCTGCTTTAGAGGACACCAAGCCCGGTGTTACCTCAAGGAGCTGAGGAGAGGAATCACCACGCTTCAATCATTCATAAGGGGACAGAAAACCAGGAAGGAGTACTCAATATTACTAGAGAGGCATAGAGCTGCTGTTGTCATACAAAAGCAGGTCAAAAGCAGGTTTGCAAGGAAGAAATTCAACAACATTTATGCTGCATCAATCGTGATACAATCAGTTCTTCGTGGCTGGTTGGTCAGAAAATGCTCAGGAAGTATAGGATTGTTGAAGCCTGGAAGCATGAAGGCTAACGAGTCAGATGAGGTTCTGGTAAAGGCATCTTTCCTTGCTGAACTACAGCGCCGCGTTCTTAAAGCTGAAGCTGCCTTGCGAGAGAAGGAAGAGGAGAATGACATCCTCCACCAACGTCTCCAACAGTATGAGAGCCGCTGGTCGGAATATGAGCTGAAAATGAAATCCATGGAAGAAGTGTGGCAGAAACAAATGAGATCCCTACAATCCAGCCTCTCAATTGCGAAGAAGAGCTTAGCCATTGATGATTCTGGGAGAAATTCTGATGCATCAGTGAATGCAAGTGATGACCGTGACCAGAGTTGGGATACAGGAAGTAACCATAGAGGCCAAGATAGCAATGGGGTGAGACCATTGAGTGCAGGTTTGAGCGTCATAAGCCGGTTAACTGAAGAATTTGATCAGAGGAGTCAAGTTTTTGGCGACGATGCCAAGTTCTTGGTGGAGGTAAAGTCTGGTAAAGTGGAAGCAAGTCTGAATCCAGACCAAGAGCTAAGAAGGTTGAAGCAGATGTTTGAGGCTTGGAAGAAGGATTACGGGGCAAGACTAAGGGAGACAAAGTTGATTATGCATAAGATTGGGAATGAAGAGGGGGGCAGTGCTGACAGGGTGAAAAAGAAGTGGTGGGGAAGGCGGAACAGCTCGAGGATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_head

Protein Analysis

1175

Amino Acids

132.2

Weight (kDa)

7.97

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_VIII-1_N PF25369 125 - 169 3.5e-23 Myosin-1-like, N-terminal SH3 domain
Myosin_head PF00063 177 - 836 1.1e-243 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 791
AarI CACCTGC 1 cut(s) 1120
AasI GACNNNNNNGTC 1 cut(s) 1460
Acc36I ACCTGC 4 cut(s) 1120, 2716, 2727, 3221
AccB1I GGYRCC 1 cut(s) 1007
AccB7I CCANNNNNTGG 1 cut(s) 158
AccI GTMKAC 4 cut(s) 76, 216, 428, 2139
AccII CGCG 1 cut(s) 2922
AciI CCGC 5 cut(s) 623, 2133, 2920, 3004, 3502
AclWI GGATC 3 cut(s) 2414, 2427, 3060
AcsI RAATTY 9 cut(s) 115, 885, 999, 1211, 1766, 2168, 2753, 3127, 3263
AcuI CTGAAG 7 cut(s) 510, 569, 1080, 1184, 1227, 2955, 3279
AdeI CACNNNGTG 1 cut(s) 2429
AfaI GTAC 7 cut(s) 578, 1154, 1588, 1861, 2286, 2548, 2678
AfiI CCNNNNNNNGG 9 cut(s) 158, 268, 269, 406, 907, 1191, 2108, 3010, 3172
AflII CTTAAG 2 cut(s) 1412, 1886
AflIII ACRYGT 2 cut(s) 721, 2566
AgeI ACCGGT 1 cut(s) 628
AjnI CCWGG 4 cut(s) 14, 2260, 2666, 2848
AleI CACNNNNGTG 1 cut(s) 984
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
Alw21I GWGCWC 1 cut(s) 1108
Alw26I GTCTC 4 cut(s) 1923, 2990, 3211, 3416
AlwI GGATC 3 cut(s) 2414, 2427, 3060
AlwNI CAGNNNCTG 4 cut(s) 86, 440, 2152, 2296
Ama87I CYCGRG 2 cut(s) 142, 3511
AoxI GGCC 5 cut(s) 18, 191, 1485, 1808, 3196
ApaI GGGCCC 1 cut(s) 22
ApeKI GCWGC 8 cut(s) 851, 1289, 2290, 2293, 2577, 2705, 2771, 2939
ApoI RAATTY 9 cut(s) 115, 885, 999, 1211, 1766, 2168, 2753, 3127, 3263
ArsI GACNNNNNNTTYG 2 cut(s) 1922, 1954
AseI ATTAAT 1 cut(s) 1665
AsiGI ACCGGT 1 cut(s) 628
Asp700I GAANNNNTTC 3 cut(s) 1000, 2322, 2753
AspLEI GCGC 2 cut(s) 440, 2919
AspS9I GGNCC 7 cut(s) 18, 19, 191, 626, 1048, 1071, 2111
AsuC2I CCSGG 2 cut(s) 54, 2599
AsuHPI GGTGA 9 cut(s) 216, 284, 356, 1076, 1234, 2441, 2623, 3226, 3490
AvaI CYCGRG 2 cut(s) 142, 3511
AvaII GGWCC 4 cut(s) 626, 1048, 1071, 2111
AxyI CCTNAGG 1 cut(s) 433
BaeGI GKGCMC 1 cut(s) 22
BamHI GGATCC 1 cut(s) 2419
BanI GGYRCC 1 cut(s) 1007
BanII GRGCYC 2 cut(s) 22, 1108
BarI GAAGNNNNNNTAC 4 cut(s) 2430, 2462, 3386, 3418
BbsI GAAGAC 4 cut(s) 261, 285, 1266, 1779
Bbv12I GWGCWC 1 cut(s) 1108
BbvCI CCTCAGC 1 cut(s) 2618
BbvI GCAGC 8 cut(s) 838, 1276, 2302, 2305, 2564, 2692, 2758, 2926
BccI CCATC 7 cut(s) 104, 257, 307, 518, 574, 1496, 1736
BceAI ACGGC 2 cut(s) 1598, 1795
BciT130I CCWGG 4 cut(s) 16, 2262, 2668, 2850
BciVI GTATCC 1 cut(s) 3169
BclI TGATCA 1 cut(s) 3268
BcnI CCSGG 2 cut(s) 54, 2599
BcoDI GTCTC 4 cut(s) 1923, 2990, 3211, 3416
BfaI CTAG 6 cut(s) 1041, 1235, 2076, 2204, 2315, 2690
BfmI CTRYAG 4 cut(s) 1290, 1353, 2288, 2912
BfoI RGCGCY 2 cut(s) 441, 2920
BfrI CTTAAG 2 cut(s) 1412, 1886
BfuAI ACCTGC 4 cut(s) 1120, 2716, 2727, 3221
BfuI GTATCC 1 cut(s) 3169
BglII AGATCT 2 cut(s) 1595, 2336
BlpI GCTNAGC 1 cut(s) 3103
BmcAI AGTACT 1 cut(s) 2678
Bme1390I CCNGG 6 cut(s) 16, 54, 2262, 2599, 2668, 2850
Bme18I GGWCC 4 cut(s) 626, 1048, 1071, 2111
BmeT110I CYCGRG 2 cut(s) 142, 3511
BmgT120I GGNCC 7 cut(s) 18, 19, 191, 626, 1048, 1071, 2111
BmiI GGNNCC 7 cut(s) 20, 21, 386, 627, 1009, 2113, 2421
BmrFI CCNGG 6 cut(s) 16, 54, 2262, 2599, 2668, 2850
BmrI ACTGGG 1 cut(s) 422
BmuI ACTGGG 1 cut(s) 422
BpiI GAAGAC 4 cut(s) 261, 285, 1266, 1779
BpmI CTGGAG 2 cut(s) 1122, 2420
Bpu10I CCTNAGC 2 cut(s) 2618, 2824
Bpu1102I GCTNAGC 1 cut(s) 3103
BpuEI CTTGAG 3 cut(s) 934, 2549, 2594
BpuMI CCSGG 2 cut(s) 54, 2599
Bsa29I ATCGAT 1 cut(s) 91
BsaAI YACGTR 1 cut(s) 2567
BsaBI GATNNNNATC 1 cut(s) 974
BsaI GGTCTC 1 cut(s) 3211
BsaJI CCNNGG 5 cut(s) 15, 266, 2260, 2554, 3036
BsaWI WCCGGW 1 cut(s) 628
BsaXI ACNNNNNCTCC 6 cut(s) 230, 260, 3305, 3335, 3413, 3443
Bsc4I CCNNNNNNNGG 9 cut(s) 158, 268, 269, 406, 907, 1191, 2108, 3010, 3172
Bse118I RCCGGY 2 cut(s) 628, 3249
Bse1I ACTGG 7 cut(s) 34, 417, 912, 997, 1594, 2452, 2515
Bse21I CCTNAGG 1 cut(s) 433
Bse3DI GCAATG 6 cut(s) 47, 136, 732, 843, 1287, 3214
Bse8I GATNNNNATC 1 cut(s) 974
BseBI CCWGG 4 cut(s) 16, 2262, 2668, 2850
BseCI ATCGAT 1 cut(s) 91
BseDI CCNNGG 5 cut(s) 15, 266, 2260, 2554, 3036
BseGI GGATG 6 cut(s) 320, 538, 1400, 1732, 1734, 2970
BseJI GATNNNNATC 1 cut(s) 974
BseLI CCNNNNNNNGG 9 cut(s) 158, 268, 269, 406, 907, 1191, 2108, 3010, 3172
BseMI GCAATG 6 cut(s) 47, 136, 732, 843, 1287, 3214
BseMII CTCAG 9 cut(s) 84, 447, 1889, 2075, 2085, 2144, 2288, 2609, 2838
BseNI ACTGG 7 cut(s) 34, 417, 912, 997, 1594, 2452, 2515
BseRI GAGGAG 5 cut(s) 130, 1853, 2635, 2975, 3289
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 8 cut(s) 838, 1276, 2302, 2305, 2564, 2692, 2758, 2926
BseYI CCCAGC 1 cut(s) 1303
BsgI GTGCAG 3 cut(s) 837, 1164, 3249
Bsh1236I CGCG 1 cut(s) 2922
BshFI GGCC 5 cut(s) 20, 193, 1487, 1810, 3198
BshNI GGYRCC 1 cut(s) 1007
BshTI ACCGGT 1 cut(s) 628
BshVI ATCGAT 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 1108
BsiHKCI CYCGRG 2 cut(s) 142, 3511
BsiSI CCGG 4 cut(s) 54, 629, 2599, 3250
BsiWI CGTACG 1 cut(s) 2546
BslFI GGGAC 5 cut(s) 639, 1810, 2018, 2097, 2670
BslI CCNNNNNNNGG 9 cut(s) 158, 268, 269, 406, 907, 1191, 2108, 3010, 3172
BsmAI GTCTC 4 cut(s) 1923, 2990, 3211, 3416
BsmBI CGTCTC 1 cut(s) 2990
BsmFI GGGAC 5 cut(s) 639, 1810, 2018, 2097, 2670
BsmI GAATGC 2 cut(s) 1605, 3151
BsnI GGCC 5 cut(s) 20, 193, 1487, 1810, 3198
Bso31I GGTCTC 1 cut(s) 3211
BsoBI CYCGRG 2 cut(s) 142, 3511
Bsp120I GGGCCC 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 22, 1108
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 7 cut(s) 88, 535, 1595, 2336, 2419, 3065, 3268
Bsp1720I GCTNAGC 1 cut(s) 3103
Bsp19I CCATGG 2 cut(s) 2554, 3036
BspACI CCGC 5 cut(s) 623, 2133, 2920, 3004, 3502
BspANI GGCC 5 cut(s) 20, 193, 1487, 1810, 3198
BspCNI CTCAG 9 cut(s) 83, 446, 1890, 2074, 2086, 2145, 2289, 2610, 2837
BspDI ATCGAT 1 cut(s) 91
BspFNI CGCG 1 cut(s) 2922
BspLI GGNNCC 7 cut(s) 20, 21, 386, 627, 1009, 2113, 2421
BspMAI CTGCAG 2 cut(s) 1294, 2292
BspMI ACCTGC 4 cut(s) 1120, 2716, 2727, 3221
BspPI GGATC 3 cut(s) 2414, 2427, 3060
BspQI GCTCTTC 1 cut(s) 3092
BspT107I GGYRCC 1 cut(s) 1007
BspTI CTTAAG 2 cut(s) 1412, 1886
BspTNI GGTCTC 1 cut(s) 3211
BsrDI GCAATG 6 cut(s) 47, 136, 732, 843, 1287, 3214
BsrFI RCCGGY 2 cut(s) 628, 3249
BsrGI TGTACA 1 cut(s) 576
BsrI ACTGG 7 cut(s) 34, 417, 912, 997, 1594, 2452, 2515
BssAI RCCGGY 2 cut(s) 628, 3249
BssECI CCNNGG 5 cut(s) 15, 266, 2260, 2554, 3036
BssMI GATC 7 cut(s) 88, 535, 1595, 2336, 2419, 3065, 3268
BssT1I CCWWGG 3 cut(s) 266, 2554, 3036
Bst2UI CCWGG 4 cut(s) 16, 2262, 2668, 2850
Bst4CI ACNGT 8 cut(s) 401, 481, 1457, 1656, 1824, 2546, 2994, 3161
Bst6I CTCTTC 4 cut(s) 1133, 2952, 3092, 3450
BstAFI CTTAAG 2 cut(s) 1412, 1886
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstAUI TGTACA 1 cut(s) 576
BstBAI YACGTR 1 cut(s) 2567
BstC8I GCNNGC 2 cut(s) 1287, 1419
BstDSI CCRYGG 2 cut(s) 2554, 3036
BstF5I GGATG 6 cut(s) 320, 538, 1400, 1732, 1734, 2970
BstFNI CGCG 1 cut(s) 2922
BstH2I RGCGCY 2 cut(s) 441, 2920
BstHHI GCGC 2 cut(s) 440, 2919
BstKTI GATC 7 cut(s) 91, 538, 1598, 2339, 2422, 3068, 3271
BstMAI GTCTC 4 cut(s) 1923, 2990, 3211, 3416
BstMBI GATC 7 cut(s) 88, 535, 1595, 2336, 2419, 3065, 3268
BstMWI GCNNNNNNNGC 7 cut(s) 743, 833, 1112, 1286, 2939, 3204, 3246
BstNI CCWGG 4 cut(s) 16, 2262, 2668, 2850
BstNSI RCATGY 2 cut(s) 725, 1530
BstSCI CCNGG 6 cut(s) 14, 52, 2260, 2597, 2666, 2848
BstSFI CTRYAG 4 cut(s) 1290, 1353, 2288, 2912
BstSLI GKGCMC 1 cut(s) 22
BstUI CGCG 1 cut(s) 2922
BstV1I GCAGC 8 cut(s) 838, 1276, 2302, 2305, 2564, 2692, 2758, 2926
BstV2I GAAGAC 4 cut(s) 261, 285, 1266, 1779
BstX2I RGATCY 4 cut(s) 1595, 2336, 2419, 3065
BstXI CCANNNNNNTGG 1 cut(s) 3307
BstYI RGATCY 4 cut(s) 1595, 2336, 2419, 3065
Bsu15I ATCGAT 1 cut(s) 91
Bsu36I CCTNAGG 1 cut(s) 433
BsuI GTATCC 1 cut(s) 3169
BsuRI GGCC 5 cut(s) 20, 193, 1487, 1810, 3198
BsuTUI ATCGAT 1 cut(s) 91
BtgI CCRYGG 2 cut(s) 2554, 3036
BtgZI GCGATG 1 cut(s) 2397
BtsCI GGATG 6 cut(s) 320, 538, 1400, 1732, 1734, 2970
BtsI GCAGTG 1 cut(s) 3472
BtsIMutI CAGTG 5 cut(s) 576, 1056, 1222, 3147, 3472
BveI ACCTGC 4 cut(s) 1120, 2716, 2727, 3221
Cac8I GCNNGC 2 cut(s) 1287, 1419
CaiI CAGNNNCTG 4 cut(s) 86, 440, 2152, 2296
CfoI GCGC 2 cut(s) 440, 2919
Cfr10I RCCGGY 2 cut(s) 628, 3249
Cfr13I GGNCC 7 cut(s) 18, 19, 191, 626, 1048, 1071, 2111
ClaI ATCGAT 1 cut(s) 91
CseI GACGC 1 cut(s) 3229
Csp6I GTAC 7 cut(s) 577, 1153, 1587, 1860, 2285, 2547, 2677
CspAI ACCGGT 1 cut(s) 628
CviQI GTAC 7 cut(s) 577, 1153, 1587, 1860, 2285, 2547, 2677
DpnI GATC 7 cut(s) 90, 537, 1597, 2338, 2421, 3067, 3270
DpnII GATC 7 cut(s) 88, 535, 1595, 2336, 2419, 3065, 3268
DraIII CACNNNGTG 1 cut(s) 2429
DrdI GACNNNNNNGTC 1 cut(s) 1460
DseDI GACNNNNNNGTC 1 cut(s) 1460
Eam1104I CTCTTC 4 cut(s) 1133, 2952, 3092, 3450
EarI CTCTTC 4 cut(s) 1133, 2952, 3092, 3450
EciI GGCGGA 2 cut(s) 2148, 3517
Ecl136II GAGCTC 1 cut(s) 1106
Eco130I CCWWGG 3 cut(s) 266, 2554, 3036
Eco24I GRGCYC 2 cut(s) 22, 1108
Eco31I GGTCTC 1 cut(s) 3211
Eco32I GATATC 2 cut(s) 517, 1615
Eco47I GGWCC 4 cut(s) 626, 1048, 1071, 2111
Eco53kI GAGCTC 1 cut(s) 1106
Eco57I CTGAAG 7 cut(s) 510, 569, 1080, 1184, 1227, 2955, 3279
Eco81I CCTNAGG 1 cut(s) 433
Eco88I CYCGRG 2 cut(s) 142, 3511
EcoICRI GAGCTC 1 cut(s) 1106
EcoO109I RGGNCCY 3 cut(s) 18, 19, 1071
EcoRI GAATTC 1 cut(s) 1211
EcoRII CCWGG 4 cut(s) 14, 2260, 2666, 2848
EcoRV GATATC 2 cut(s) 517, 1615
EcoT14I CCWWGG 3 cut(s) 266, 2554, 3036
EcoT22I ATGCAT 3 cut(s) 1528, 3139, 3441
EcoT38I GRGCYC 2 cut(s) 22, 1108
ErhI CCWWGG 3 cut(s) 266, 2554, 3036
Esp3I CGTCTC 1 cut(s) 2990
FalI AAGNNNNNCTT 6 cut(s) 1489, 1521, 1773, 1805, 3086, 3118
FaqI GGGAC 5 cut(s) 639, 1810, 2018, 2097, 2670
FauI CCCGC 1 cut(s) 616
FauNDI CATATG 2 cut(s) 229, 1972
FbaI TGATCA 1 cut(s) 3268
FblI GTMKAC 4 cut(s) 76, 216, 428, 2139
FokI GGATG 6 cut(s) 307, 545, 1407, 1721, 1739, 2957
FriOI GRGCYC 2 cut(s) 22, 1108
FspBI CTAG 6 cut(s) 1041, 1235, 2076, 2204, 2315, 2690
GlaI GCGC 2 cut(s) 439, 2918
GsaI CCCAGC 1 cut(s) 1307
GsuI CTGGAG 2 cut(s) 1122, 2420
HaeII RGCGCY 2 cut(s) 441, 2920
HaeIII GGCC 5 cut(s) 20, 193, 1487, 1810, 3198
HapII CCGG 4 cut(s) 54, 629, 2599, 3250
HgaI GACGC 1 cut(s) 3229
HhaI GCGC 2 cut(s) 440, 2919
Hin6I GCGC 2 cut(s) 438, 2917
HinP1I GCGC 2 cut(s) 438, 2917
HincII GTYRAC 1 cut(s) 3255
HindII GTYRAC 1 cut(s) 3255
HindIII AAGCTT 3 cut(s) 371, 1415, 1883
HpaI GTTAAC 1 cut(s) 3255
HpaII CCGG 4 cut(s) 54, 629, 2599, 3250
HphI GGTGA 9 cut(s) 216, 284, 356, 1076, 1234, 2441, 2623, 3226, 3490
Hpy166II GTNNAC 7 cut(s) 77, 217, 429, 719, 1845, 2140, 3255
Hpy8I GTNNAC 7 cut(s) 77, 217, 429, 719, 1845, 2140, 3255
Hpy99I CGWCG 1 cut(s) 3296
HpyCH4III ACNGT 8 cut(s) 401, 481, 1457, 1656, 1824, 2546, 2994, 3161
HpyCH4IV ACGT 4 cut(s) 2200, 2230, 2566, 2983
HpyF10VI GCNNNNNNNGC 7 cut(s) 743, 833, 1112, 1286, 2939, 3204, 3246
HpySE526I ACGT 4 cut(s) 2200, 2230, 2566, 2983
HspAI GCGC 2 cut(s) 438, 2917
Ksp22I TGATCA 1 cut(s) 3268
KspAI GTTAAC 1 cut(s) 3255
Kzo9I GATC 7 cut(s) 88, 535, 1595, 2336, 2419, 3065, 3268
LguI GCTCTTC 1 cut(s) 3092
LmnI GCTCC 6 cut(s) 301, 806, 1103, 1111, 2556, 2614
Lsp1109I GCAGC 8 cut(s) 838, 1276, 2302, 2305, 2564, 2692, 2758, 2926
MaeI CTAG 6 cut(s) 1041, 1235, 2076, 2204, 2315, 2690
MaeII ACGT 4 cut(s) 2200, 2230, 2566, 2983
MaeIII GTNAC 8 cut(s) 344, 475, 551, 1703, 2358, 2603, 3161, 3185
MalI GATC 7 cut(s) 90, 537, 1597, 2338, 2421, 3067, 3270
MboI GATC 7 cut(s) 88, 535, 1595, 2336, 2419, 3065, 3268
MfeI CAATTG 3 cut(s) 1174, 2180, 3087
MflI RGATCY 4 cut(s) 1595, 2336, 2419, 3065
MhlI GDGCHC 2 cut(s) 22, 1108
MlyI GAGTC 6 cut(s) 1850, 2135, 2185, 2274, 2879, 3286
MmeI TCCRAC 3 cut(s) 412, 2991, 3013
Mph1103I ATGCAT 3 cut(s) 1528, 3139, 3441
MroXI GAANNNNTTC 3 cut(s) 1000, 2322, 2753
MslI CAYNNNNRTG 3 cut(s) 726, 984, 2047
MspA1I CMGCKG 2 cut(s) 2296, 3006
MspCI CTTAAG 2 cut(s) 1412, 1886
MspI CCGG 4 cut(s) 54, 629, 2599, 3250
MspR9I CCNGG 6 cut(s) 16, 54, 2262, 2599, 2668, 2850
MunI CAATTG 3 cut(s) 1174, 2180, 3087
Mva1269I GAATGC 2 cut(s) 1605, 3151
MvaI CCWGG 4 cut(s) 16, 2262, 2668, 2850
MvnI CGCG 1 cut(s) 2922
MwoI GCNNNNNNNGC 7 cut(s) 743, 833, 1112, 1286, 2939, 3204, 3246
NciI CCSGG 2 cut(s) 54, 2599
NcoI CCATGG 2 cut(s) 2554, 3036
NdeI CATATG 2 cut(s) 229, 1972
NdeII GATC 7 cut(s) 88, 535, 1595, 2336, 2419, 3065, 3268
NlaIV GGNNCC 7 cut(s) 20, 21, 386, 627, 1009, 2113, 2421
NmuCI GTSAC 4 cut(s) 344, 1703, 2358, 3161
NsiI ATGCAT 3 cut(s) 1528, 3139, 3441
NspI RCATGY 2 cut(s) 725, 1530
OliI CACNNNNGTG 1 cut(s) 984
PaeR7I CTCGAG 2 cut(s) 142, 3511
PaqCI CACCTGC 1 cut(s) 1120
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 3092
PctI GAATGC 2 cut(s) 1605, 3151
PdmI GAANNNNTTC 3 cut(s) 1000, 2322, 2753
PfeI GAWTC 8 cut(s) 221, 287, 443, 1628, 2021, 2628, 3116, 3346
Pfl23II CGTACG 1 cut(s) 2546
PflMI CCANNNNNTGG 1 cut(s) 158
PinAI ACCGGT 1 cut(s) 628
PleI GAGTC 6 cut(s) 1849, 2135, 2184, 2274, 2878, 3285
PpsI GAGTC 6 cut(s) 1849, 2135, 2184, 2274, 2878, 3285
Ppu21I YACGTR 1 cut(s) 2567
PpuMI RGGWCCY 1 cut(s) 1071
PscI ACATGT 1 cut(s) 721
PshBI ATTAAT 1 cut(s) 1665
PsiI TTATAA 1 cut(s) 791
Psp124BI GAGCTC 1 cut(s) 1108
Psp5II RGGWCCY 1 cut(s) 1071
Psp6I CCWGG 4 cut(s) 14, 2260, 2666, 2848
PspFI CCCAGC 1 cut(s) 1303
PspGI CCWGG 4 cut(s) 14, 2260, 2666, 2848
PspLI CGTACG 1 cut(s) 2546
PspN4I GGNNCC 7 cut(s) 20, 21, 386, 627, 1009, 2113, 2421
PspOMI GGGCCC 1 cut(s) 18
PspPI GGNCC 7 cut(s) 18, 19, 191, 626, 1048, 1071, 2111
PspPPI RGGWCCY 1 cut(s) 1071
PspXI VCTCGAGB 2 cut(s) 142, 3511
PstI CTGCAG 2 cut(s) 1294, 2292
PstNI CAGNNNCTG 4 cut(s) 86, 440, 2152, 2296
PsuI RGATCY 4 cut(s) 1595, 2336, 2419, 3065
PvuII CAGCTG 1 cut(s) 2296
RsaI GTAC 7 cut(s) 578, 1154, 1588, 1861, 2286, 2548, 2678
RsaNI GTAC 7 cut(s) 577, 1153, 1587, 1860, 2285, 2547, 2677
RseI CAYNNNNRTG 3 cut(s) 726, 984, 2047
SacI GAGCTC 1 cut(s) 1108
SapI GCTCTTC 1 cut(s) 3092
Sau3AI GATC 7 cut(s) 88, 535, 1595, 2336, 2419, 3065, 3268
Sau96I GGNCC 7 cut(s) 18, 19, 191, 626, 1048, 1071, 2111
ScaI AGTACT 1 cut(s) 2678
SchI GAGTC 6 cut(s) 1850, 2135, 2185, 2274, 2879, 3286
ScrFI CCNGG 6 cut(s) 16, 54, 2262, 2599, 2668, 2850
SduI GDGCHC 2 cut(s) 22, 1108
SfcI CTRYAG 4 cut(s) 1290, 1353, 2288, 2912
Sfr274I CTCGAG 2 cut(s) 142, 3511
SinI GGWCC 4 cut(s) 626, 1048, 1071, 2111
SlaI CTCGAG 2 cut(s) 142, 3511
SmiMI CAYNNNNRTG 3 cut(s) 726, 984, 2047
SmlI CTYRAG 7 cut(s) 142, 949, 1412, 1886, 2528, 2609, 3511
SmoI CTYRAG 7 cut(s) 142, 949, 1412, 1886, 2528, 2609, 3511
SsiI CCGC 5 cut(s) 623, 2133, 2920, 3004, 3502
SspI AATATT 4 cut(s) 423, 845, 1015, 2685
SspMI CTAG 6 cut(s) 1041, 1235, 2076, 2204, 2315, 2690
SstI GAGCTC 1 cut(s) 1108
StyD4I CCNGG 6 cut(s) 14, 52, 2260, 2597, 2666, 2848
StyI CCWWGG 3 cut(s) 266, 2554, 3036
TaaI ACNGT 8 cut(s) 401, 481, 1457, 1656, 1824, 2546, 2994, 3161
TaiI ACGT 4 cut(s) 2203, 2233, 2569, 2986
TaqI TCGA 7 cut(s) 91, 143, 252, 1432, 1491, 2089, 3512
TatI WGTACW 2 cut(s) 576, 2676
TauI GCSGC 2 cut(s) 2922, 3006
TfiI GAWTC 8 cut(s) 221, 287, 443, 1628, 2021, 2628, 3116, 3346
TscAI CASTG 5 cut(s) 576, 1063, 1222, 3147, 3472
TseFI GTSAC 4 cut(s) 344, 1703, 2358, 3161
TseI GCWGC 8 cut(s) 851, 1289, 2290, 2293, 2577, 2705, 2771, 2939
Tsp45I GTSAC 4 cut(s) 344, 1703, 2358, 3161
TspRI CASTG 5 cut(s) 576, 1063, 1222, 3147, 3472
Van91I CCANNNNNTGG 1 cut(s) 158
Vha464I CTTAAG 2 cut(s) 1412, 1886
VpaK11BI GGWCC 4 cut(s) 626, 1048, 1071, 2111
VspI ATTAAT 1 cut(s) 1665
XapI RAATTY 9 cut(s) 115, 885, 999, 1211, 1766, 2168, 2753, 3127, 3263
XbaI TCTAGA 4 cut(s) 1040, 1234, 2203, 2314
XceI RCATGY 2 cut(s) 725, 1530
XcmI CCANNNNNNNNNTGG 5 cut(s) 402, 754, 1787, 3206, 3307
XhoI CTCGAG 2 cut(s) 142, 3511
XmiI GTMKAC 4 cut(s) 76, 216, 428, 2139
XmnI GAANNNNTTC 3 cut(s) 1000, 2322, 2753
XspI CTAG 6 cut(s) 1041, 1235, 2076, 2204, 2315, 2690
ZrmI AGTACT 1 cut(s) 2678
Zsp2I ATGCAT 3 cut(s) 1528, 3139, 3441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.