RchiOBHm_Chr4g0446931
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005277
Physical Location & Seq
Forward (+)
66878477 .. 66888995
10519 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41441

Sequence Viewer

Length: 3534 bp
ATGTCACAGAAATCTACGTCCACACCTTCCTTCCAGTCAATCAAATCATTGCCTGGTGATTTTAGGTTCTCAGGCGCACCGGCTTCTGATCGATTTGGAGATGACGATAATGTCAGAAATAGTAATATCATCTCCTCCAGTATTCCAGAAAATGGTGAATTAAAGGATAGCACTGCTGAAGGGGTTGAAGGTAGTCCCGGGCCTCTTGGTGACACGGACCAAGTAATTGATGATTCACCATATACCGGCGACACTATCTCCACTGAAGACAGGCAGTCAAGTGTTGATGAAGACCTGGATTCTGTAACTCCACCTTTACCCTCAATATCTTCATTTCGCACAGAACGTCGGTGGGGTGACACCACGTCATATGCTGGGAAGAAGAAGCTACAATCTTGGTACCAACTTCCAAATGGGAATTGGGAACTGGGGAAAATATTGTCGACTTCAGTCACTGAAACTGTTATATCACTGCCTAACGAGAAGGTTATAAAGGTAAAAACTGAAGATTTGGTTCCAGCAAATCCTGATATCCTTAATGGTGTGGATGATCTCATGCAACTTAGTTACTTAAACGAACCATCAGTGCTGTACAATCTTCAGTATAGATACAATCGAGATATGATTTATACAAAAGCGGGACCAGTGTTGGTTGCTATCAACCCCTTTAAGAAAGTTTGTTTATATGGGAACGAATACATTGAAGCATATAAGCATAAAGCTGTTGATAGTCCACATGTATATGCCATCACAGACACAGCCATCCGAGAAATGGTTCGAGATGAAGTAAATCAATCGATAATTATAAGTGGGGAAAGTGGAGCAGGAAAAACTGAAACTGCAAAGATAGCAATGCAATATTTGGCCGCACTTGGAGGTGGAAGTGGAATAGAACATGAAATATTGAAAACTAATCCGATACTAGAAGCCTTTGGTAATGCGAAAACATTAAGAAATGACAACTCAAGTCGCTTTGGAAAATTGATTGAAATCCACTTTAGTGAAACTGGAAAGATATCTGGTGCCAACATTCAAACTTTTTTGCTTGAAAAGTCTAGAGTCGTCCAATGCACTGAGGGAGAAAGGTCATACCATATATTTTATCAGCTGTGTGCTGGAGCTCCACCTGCTCTTAGAGAGATGCTGAACTTGAAGAGTGCAGATGAGTACAAATATCTGCAGCAAAGCAATTGTTATTCCATTACTGGAGTTGATGATGCTGAAGAATTTCGTGTGGTCAAGGAAGCTCTAGATGTCGTCCATATTAACAAGGAAGACCAAGAAAGTGTCTTTGCAATGCTTGCTGCAGTCTTGTGGCTGGGAAACATCTCATTTAGTGTGATTGATAATGAAAATCATGTGGAAGCTGTGGCAGATGAAGGTTTGTTTACTGTTGCTAAATTGGTTGGATGTTGCCCGGAGGAGCTTATGCTTGCTTTATCAACTCGCAAAATGCGAGTTGGTAATGACAATATTGTCCAAAAGCTAACACTATCACAGGCTGTCGATACGAGAGATGCTTTGGCAAAGTCGATCTATGCATGTTTGTTTGAGTGGTTGGTTGAACAAATTAACAAATCACTTGCAGTAGGAAAGCGGCGTACTGGCAGATCTATTAGCATTCTGGATATCTATGGTTTTGAATCCTTTGATAGAAATAGTTTTGAACAATTCTGCATTAATTATGCAAATGAGAGATTGCAGCAGCACTTCAATCGCCACTTGTTCAAACTAGAGCAGGAGGAATATATTCAGGATGGCATTGATTGGACAAGAGTTGAATTTGAAGACAACCAAGATTGCCTTGGCCTTTTTGAAAAGAGACCGTTGGGATTACTGTCCCTCTTAGATGAAGAGTCCACTTTCCCAAATGGTAGTGATTTGACCTTCGCCAACAAGCTTAAGCAACATCTGAGTTCTAATTCTTCTTTTAGAGGAGAACGAGACAAAGCCTTTACTATCAGTCATTATGCTGGAGAGGTTACGTATGACACAACAGGTTTTTTGGAGAAGAATAGAGACTTGCTGCATTTGGATTCCATTCAGCTTCTTTCCTCATGCTCATGCCACCTTCCTCAGATATTTGCATCTAGTATGCTCAATCGATCTGAGAAGCCTGTAGTTGGCCCCTTGCATAAATTAGGTGGAGCAGACTCCCAGAAGCTGAGTGTTGCAACAAAATTTAAGGGCCAATTGTTCCTACTGATGAAACGTCTTGAGAACACCACTCCACATTTCATACGTTGTATCAAGCCCAACAATAGTCAATCTCCAGGAATATATGAGCAAGGACTTGTCCTGCAGCAGCTAAGATGCTGTGGAGTCCTTGAAGTGGTTCGAATATCGAGATCTGGCTTTCCTACCAGAATGTCTCACCAGAAGTTTGCAAGAAGATATGGTTTTCTTCTGCTGGAGAATGTTGCATCACAGGAGCCACTTAGTGTTTCAGTTGCTATTCTTCATCAGTTCAATATATTACCAGAGATGTATCAAGTGGGCTATACAAAATTGTTTTTCCGTACTGGACAGATTGGGGTGCTTGAGGATACCAGAAATCGTACACTCCATGGTATTTTACGCGTTCAAAGCTGCTTTAGAGGACACCAAGCTCGATGTTACCTTAAGGAGCTGAGAAGAGGAATCACCACGCTTCAATCCTTTGTAAGGGGAGAGAAAACGAGAAAGGAGTATGCAGTTTTACTGCAGAGGCATAGAGCTGCTGTATTCATACAAAAGCTGATGAAAAGCAGGATTGCAAGGAAGAAATTCATTAACATTTGTGATGCATCAATCGTGATACAATCAGTTTATCGTGGCTGGTTTGTCAGAAGATGCTCAGGAGGTATAGGATTAATGAAACCTGGGAGCACAAAGGCGAACGAGTCAGATGAGGTGCTGGTCAAATCATCTTTTCTAGCTGAATTACAACGTCGTGTTCTTAAGGCTGAAGCTGCCTTGAGAGAGAAGGAAGAGGAGAACGACATTCTCCACCAACGCCTCCAACAGTATGAGAACCGCTGGTCTGAATATGAGCTGAAAATGAAATCCATGGAAGAAGTATGGCAGAAACAAATGAGATCCCTGCAATCCAGCCTCTCAATTGCCAAGAAGAGCCTTGCCATTGATGATTCTGAGAGAAATTCTGATGCATCGGTCAATGCAAGTGATGATCGCGACTACAGTTGGGATACAGGAAGCAACCATAAAGGCCAAGATAGCAATGGGGGAAGACCCATGAGTGCCGGTTTGAGTGTTATAAGCCGGTTGACTGAAGAATTTGACCAGAGGAGTCAAGTATTTGGTGATGACGCCAAGTTCTTGGTGGAGGTAAAATCTGGTCAAGTTGAAGCAAGTCTGAATCCAGACCGAGAGCTTAGAAGGTTGAAGCAGATGTTTGAGGCTTGGAAGAAGGATTATGGTGCAAGACTTCGGGAGACAAAGGTGGTGTTGACTAAGCTTGGAAATGAAGAGGGTACTGTTGATAGGGCAAAAAAGAAGTGGTGGGGAAGGAGGAACAGCTCGAGGATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_head

Protein Analysis

1177

Amino Acids

132.89

Weight (kDa)

6.91

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_VIII-1_N PF25369 130 - 174 2e-24 Myosin-1-like, N-terminal SH3 domain
Myosin_head PF00063 182 - 840 6.4e-243 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 491, 806, 3257
AarI CACCTGC 1 cut(s) 1135
AasI GACNNNNNNGTC 2 cut(s) 110, 1475
Acc36I ACCTGC 1 cut(s) 1135
Acc65I GGTACC 1 cut(s) 399
AccB1I GGYRCC 2 cut(s) 399, 1022
AccB7I CCANNNNNTGG 1 cut(s) 152
AccI GTMKAC 1 cut(s) 443
AccII CGCG 2 cut(s) 2580, 3174
AciI CCGC 4 cut(s) 638, 867, 1597, 3016
AclWI GGATC 1 cut(s) 3072
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 6 cut(s) 1226, 1781, 2180, 2765, 3139, 3275
AcuI CTGAAG 8 cut(s) 198, 285, 432, 525, 584, 1242, 2967, 3291
AcyI GRCGYC 1 cut(s) 3309
AdeI CACNNNGTG 1 cut(s) 2441
AfaI GTAC 7 cut(s) 401, 593, 1169, 1603, 2521, 2560, 3475
AfiI CCNNNNNNNGG 6 cut(s) 152, 245, 772, 2123, 2332, 2664
AflII CTTAAG 3 cut(s) 1901, 2621, 2939
AflIII ACRYGT 2 cut(s) 736, 2578
AhdI GACNNNNNGTC 2 cut(s) 274, 364
AjiI CACGTC 1 cut(s) 366
AjnI CCWGG 4 cut(s) 52, 294, 2272, 2860
AleI CACNNNNGTG 1 cut(s) 999
Alw21I GWGCWC 2 cut(s) 1123, 2870
Alw26I GTCTC 5 cut(s) 1816, 1938, 2013, 2376, 3428
AlwI GGATC 1 cut(s) 3072
AlwNI CAGNNNCTG 2 cut(s) 455, 2164
Ama87I CYCGRG 2 cut(s) 197, 3520
AoxI GGCC 6 cut(s) 200, 864, 1807, 2125, 2188, 3208
ApoI RAATTY 6 cut(s) 1226, 1781, 2180, 2765, 3139, 3275
AseI ATTAAT 2 cut(s) 1680, 2852
Asp700I GAANNNNTTC 5 cut(s) 774, 1227, 1749, 2334, 2765
Asp718I GGTACC 1 cut(s) 399
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 5 cut(s) 200, 217, 641, 2126, 2188
AsuC2I CCSGG 3 cut(s) 198, 199, 1418
AsuHPI GGTGA 8 cut(s) 68, 167, 221, 228, 368, 2366, 2635, 3314
AsuII TTCGAA 1 cut(s) 2338
AvaI CYCGRG 2 cut(s) 197, 3520
AvaII GGWCC 2 cut(s) 217, 641
BanI GGYRCC 2 cut(s) 399, 1022
BanII GRGCYC 1 cut(s) 1123
BbsI GAAGAC 5 cut(s) 273, 297, 1281, 1794, 3235
Bbv12I GWGCWC 2 cut(s) 1123, 2870
BccI CCATC 4 cut(s) 589, 755, 770, 1751
BcgI CGANNNNNNTGC 2 cut(s) 1697, 1731
BciT130I CCWGG 4 cut(s) 54, 296, 2274, 2862
BciVI GTATCC 2 cut(s) 2539, 3181
BcnI CCSGG 3 cut(s) 198, 199, 1418
BcoDI GTCTC 5 cut(s) 1816, 1938, 2013, 2376, 3428
BfaI CTAG 6 cut(s) 923, 1056, 1250, 1733, 2091, 2915
BfmI CTRYAG 6 cut(s) 1178, 1305, 2118, 2300, 2702, 3178
BfrI CTTAAG 3 cut(s) 1901, 2621, 2939
BfuAI ACCTGC 1 cut(s) 1135
BfuI GTATCC 2 cut(s) 2539, 3181
BglII AGATCT 2 cut(s) 1610, 2348
Bme1390I CCNGG 7 cut(s) 54, 198, 199, 296, 1418, 2274, 2862
Bme18I GGWCC 2 cut(s) 217, 641
BmeRI GACNNNNNGTC 2 cut(s) 274, 364
BmeT110I CYCGRG 2 cut(s) 197, 3520
BmgBI CACGTC 1 cut(s) 366
BmgT120I GGNCC 5 cut(s) 200, 217, 641, 2126, 2188
BmiI GGNNCC 6 cut(s) 401, 516, 642, 1024, 2128, 2433
BmrFI CCNGG 7 cut(s) 54, 198, 199, 296, 1418, 2274, 2862
BmrI ACTGGG 1 cut(s) 437
BmuI ACTGGG 1 cut(s) 437
BoxI GACNNNNGTC 1 cut(s) 449
BpiI GAAGAC 5 cut(s) 273, 297, 1281, 1794, 3235
BpmI CTGGAG 6 cut(s) 121, 1137, 1227, 1995, 2256, 2432
Bpu10I CCTNAGC 1 cut(s) 2836
Bpu14I TTCGAA 1 cut(s) 2338
BpuEI CTTGAG 4 cut(s) 949, 2237, 2561, 2977
BpuMI CCSGG 3 cut(s) 198, 199, 1418
Bsa29I ATCGAT 3 cut(s) 91, 797, 2104
BsaAI YACGTR 1 cut(s) 1986
BsaBI GATNNNNATC 1 cut(s) 989
BsaHI GRCGYC 1 cut(s) 3309
BsaI GGTCTC 1 cut(s) 1816
BsaJI CCNNGG 5 cut(s) 197, 1804, 2566, 2861, 3048
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 6 cut(s) 152, 245, 772, 2123, 2332, 2664
Bse118I RCCGGY 4 cut(s) 79, 245, 3242, 3261
Bse1I ACTGG 8 cut(s) 34, 138, 432, 644, 1012, 1210, 1609, 2527
Bse3DI GCAATG 4 cut(s) 47, 858, 1302, 3226
Bse8I GATNNNNATC 1 cut(s) 989
BseBI CCWGG 4 cut(s) 54, 296, 2274, 2862
BseCI ATCGAT 3 cut(s) 91, 797, 2104
BseDI CCNNGG 5 cut(s) 197, 1804, 2566, 2861, 3048
BseGI GGATG 4 cut(s) 553, 762, 1415, 1762
BseJI GATNNNNATC 1 cut(s) 989
BseLI CCNNNNNNNGG 6 cut(s) 152, 245, 772, 2123, 2332, 2664
BseMI GCAATG 4 cut(s) 47, 858, 1302, 3226
BseMII CTCAG 9 cut(s) 84, 1065, 1904, 2090, 2100, 2156, 2621, 2850, 3123
BseNI ACTGG 8 cut(s) 34, 138, 432, 644, 1012, 1210, 1609, 2527
BseRI GAGGAG 5 cut(s) 124, 1436, 1950, 2987, 3301
BseYI CCCAGC 2 cut(s) 374, 1318
BsgI GTGCAG 1 cut(s) 1179
Bsh1236I CGCG 2 cut(s) 2580, 3174
BshFI GGCC 6 cut(s) 202, 866, 1809, 2127, 2190, 3210
BshNI GGYRCC 2 cut(s) 399, 1022
BshVI ATCGAT 3 cut(s) 91, 797, 2104
BsiHKAI GWGCWC 2 cut(s) 1123, 2870
BsiHKCI CYCGRG 2 cut(s) 197, 3520
BsiSI CCGG 6 cut(s) 80, 198, 246, 1418, 3243, 3262
BslFI GGGAC 3 cut(s) 180, 654, 1825
BslI CCNNNNNNNGG 6 cut(s) 152, 245, 772, 2123, 2332, 2664
BsmAI GTCTC 5 cut(s) 1816, 1938, 2013, 2376, 3428
BsmFI GGGAC 3 cut(s) 180, 654, 1825
BsmI GAATGC 1 cut(s) 1620
BsnI GGCC 6 cut(s) 202, 866, 1809, 2127, 2190, 3210
Bso31I GGTCTC 1 cut(s) 1816
BsoBI CYCGRG 2 cut(s) 197, 3520
Bsp119I TTCGAA 1 cut(s) 2338
Bsp1286I GDGCHC 2 cut(s) 1123, 2870
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 8 cut(s) 88, 550, 1533, 1610, 2105, 2348, 3077, 3169
Bsp19I CCATGG 2 cut(s) 2566, 3048
Bsp68I TCGCGA 1 cut(s) 3174
BspACI CCGC 4 cut(s) 638, 867, 1597, 3016
BspANI GGCC 6 cut(s) 202, 866, 1809, 2127, 2190, 3210
BspCNI CTCAG 9 cut(s) 83, 1066, 1905, 2089, 2101, 2157, 2622, 2849, 3124
BspDI ATCGAT 3 cut(s) 91, 797, 2104
BspFNI CGCG 2 cut(s) 2580, 3174
BspLI GGNNCC 6 cut(s) 401, 516, 642, 1024, 2128, 2433
BspMAI CTGCAG 4 cut(s) 1182, 1309, 2304, 2706
BspMI ACCTGC 1 cut(s) 1135
BspPI GGATC 1 cut(s) 3072
BspQI GCTCTTC 1 cut(s) 3104
BspT104I TTCGAA 1 cut(s) 2338
BspT107I GGYRCC 2 cut(s) 399, 1022
BspTI CTTAAG 3 cut(s) 1901, 2621, 2939
BspTNI GGTCTC 1 cut(s) 1816
BsrDI GCAATG 4 cut(s) 47, 858, 1302, 3226
BsrFI RCCGGY 4 cut(s) 79, 245, 3242, 3261
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 8 cut(s) 34, 138, 432, 644, 1012, 1210, 1609, 2527
BssAI RCCGGY 4 cut(s) 79, 245, 3242, 3261
BssECI CCNNGG 5 cut(s) 197, 1804, 2566, 2861, 3048
BssMI GATC 8 cut(s) 88, 550, 1533, 1610, 2105, 2348, 3077, 3169
BssNI GRCGYC 1 cut(s) 3309
BssT1I CCWWGG 3 cut(s) 1804, 2566, 3048
Bst2UI CCWGG 4 cut(s) 54, 296, 2274, 2862
Bst4CI ACNGT 7 cut(s) 463, 1393, 1827, 1839, 3006, 3182, 3478
Bst6I CTCTTC 6 cut(s) 1148, 1848, 2629, 2964, 3104, 3462
BstACI GRCGYC 1 cut(s) 3309
BstAFI CTTAAG 3 cut(s) 1901, 2621, 2939
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstAUI TGTACA 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 1986
BstBI TTCGAA 1 cut(s) 2338
BstC8I GCNNGC 2 cut(s) 1302, 1434
BstDSI CCRYGG 2 cut(s) 2566, 3048
BstENI CCTNNNNNAGG 1 cut(s) 2662
BstF5I GGATG 4 cut(s) 553, 762, 1415, 1762
BstFNI CGCG 2 cut(s) 2580, 3174
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 8 cut(s) 91, 553, 1536, 1613, 2108, 2351, 3080, 3172
BstMAI GTCTC 5 cut(s) 1816, 1938, 2013, 2376, 3428
BstMBI GATC 8 cut(s) 88, 550, 1533, 1610, 2105, 2348, 3077, 3169
BstMWI GCNNNNNNNGC 6 cut(s) 848, 1127, 1301, 2586, 2951, 3216
BstNI CCWGG 4 cut(s) 54, 296, 2274, 2862
BstNSI RCATGY 2 cut(s) 740, 1545
BstPAI GACNNNNGTC 1 cut(s) 449
BstSCI CCNGG 7 cut(s) 52, 196, 197, 294, 1416, 2272, 2860
BstSFI CTRYAG 6 cut(s) 1178, 1305, 2118, 2300, 2702, 3178
BstSNI TACGTA 1 cut(s) 1986
BstUI CGCG 2 cut(s) 2580, 3174
BstV2I GAAGAC 5 cut(s) 273, 297, 1281, 1794, 3235
BstX2I RGATCY 3 cut(s) 1610, 2348, 3077
BstXI CCANNNNNNTGG 1 cut(s) 3319
BstYI RGATCY 3 cut(s) 1610, 2348, 3077
Bsu15I ATCGAT 3 cut(s) 91, 797, 2104
BsuI GTATCC 2 cut(s) 2539, 3181
BsuRI GGCC 6 cut(s) 202, 866, 1809, 2127, 2190, 3210
BsuTUI ATCGAT 3 cut(s) 91, 797, 2104
BtgI CCRYGG 2 cut(s) 2566, 3048
BtrI CACGTC 1 cut(s) 366
BtsCI GGATG 4 cut(s) 553, 762, 1415, 1762
BtsI GCAGTG 2 cut(s) 171, 470
BtsIMutI CAGTG 7 cut(s) 171, 261, 453, 470, 591, 651, 1071
BtuMI TCGCGA 1 cut(s) 3174
BveI ACCTGC 1 cut(s) 1135
Cac8I GCNNGC 2 cut(s) 1302, 1434
CaiI CAGNNNCTG 2 cut(s) 455, 2164
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 4 cut(s) 79, 245, 3242, 3261
Cfr13I GGNCC 5 cut(s) 200, 217, 641, 2126, 2188
Cfr9I CCCGGG 1 cut(s) 197
ClaI ATCGAT 3 cut(s) 91, 797, 2104
CseI GACGC 1 cut(s) 3317
Csp6I GTAC 7 cut(s) 400, 592, 1168, 1602, 2520, 2559, 3474
CviQI GTAC 7 cut(s) 400, 592, 1168, 1602, 2520, 2559, 3474
DpnI GATC 8 cut(s) 90, 552, 1535, 1612, 2107, 2350, 3079, 3171
DpnII GATC 8 cut(s) 88, 550, 1533, 1610, 2105, 2348, 3077, 3169
DraIII CACNNNGTG 1 cut(s) 2441
DrdI GACNNNNNNGTC 2 cut(s) 110, 1475
DriI GACNNNNNGTC 2 cut(s) 274, 364
DseDI GACNNNNNNGTC 2 cut(s) 110, 1475
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 6 cut(s) 1148, 1848, 2629, 2964, 3104, 3462
Eam1105I GACNNNNNGTC 2 cut(s) 274, 364
EarI CTCTTC 6 cut(s) 1148, 1848, 2629, 2964, 3104, 3462
Ecl136II GAGCTC 1 cut(s) 1121
Eco105I TACGTA 1 cut(s) 1986
Eco130I CCWWGG 3 cut(s) 1804, 2566, 3048
Eco24I GRGCYC 1 cut(s) 1123
Eco31I GGTCTC 1 cut(s) 1816
Eco32I GATATC 3 cut(s) 532, 1017, 1630
Eco47I GGWCC 2 cut(s) 217, 641
Eco53kI GAGCTC 1 cut(s) 1121
Eco57I CTGAAG 8 cut(s) 198, 285, 432, 525, 584, 1242, 2967, 3291
Eco88I CYCGRG 2 cut(s) 197, 3520
EcoICRI GAGCTC 1 cut(s) 1121
EcoNI CCTNNNNNAGG 1 cut(s) 2662
EcoRII CCWGG 4 cut(s) 52, 294, 2272, 2860
EcoRV GATATC 3 cut(s) 532, 1017, 1630
EcoT14I CCWWGG 3 cut(s) 1804, 2566, 3048
EcoT22I ATGCAT 3 cut(s) 1543, 2788, 3151
EcoT38I GRGCYC 1 cut(s) 1123
ErhI CCWWGG 3 cut(s) 1804, 2566, 3048
FalI AAGNNNNNCTT 2 cut(s) 1788, 1820
FaqI GGGAC 3 cut(s) 180, 654, 1825
FauI CCCGC 1 cut(s) 631
FauNDI CATATG 1 cut(s) 370
FblI GTMKAC 1 cut(s) 443
FokI GGATG 4 cut(s) 560, 749, 1422, 1769
FriOI GRGCYC 1 cut(s) 1123
FspBI CTAG 6 cut(s) 923, 1056, 1250, 1733, 2091, 2915
GlaI GCGC 1 cut(s) 76
GsaI CCCAGC 2 cut(s) 378, 1322
GsuI CTGGAG 6 cut(s) 121, 1137, 1227, 1995, 2256, 2432
HaeIII GGCC 6 cut(s) 202, 866, 1809, 2127, 2190, 3210
HapII CCGG 6 cut(s) 80, 198, 246, 1418, 3243, 3262
HgaI GACGC 1 cut(s) 3317
HhaI GCGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 3309
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 3 cut(s) 444, 3267, 3450
HindII GTYRAC 3 cut(s) 444, 3267, 3450
HindIII AAGCTT 2 cut(s) 1898, 3455
HpaII CCGG 6 cut(s) 80, 198, 246, 1418, 3243, 3262
HphI GGTGA 8 cut(s) 68, 167, 221, 228, 368, 2366, 2635, 3314
Hpy166II GTNNAC 8 cut(s) 21, 444, 734, 1389, 1860, 2561, 3267, 3450
Hpy8I GTNNAC 8 cut(s) 21, 444, 734, 1389, 1860, 2561, 3267, 3450
Hpy99I CGWCG 2 cut(s) 351, 2934
HpyCH4III ACNGT 7 cut(s) 463, 1393, 1827, 1839, 3006, 3182, 3478
HpyCH4IV ACGT 7 cut(s) 17, 346, 365, 1985, 2212, 2242, 2929
HpyF10VI GCNNNNNNNGC 6 cut(s) 848, 1127, 1301, 2586, 2951, 3216
HpySE526I ACGT 7 cut(s) 17, 346, 365, 1985, 2212, 2242, 2929
Hsp92I GRCGYC 1 cut(s) 3309
HspAI GCGC 1 cut(s) 75
KpnI GGTACC 1 cut(s) 403
Kzo9I GATC 8 cut(s) 88, 550, 1533, 1610, 2105, 2348, 3077, 3169
LguI GCTCTTC 1 cut(s) 3104
LmnI GCTCC 8 cut(s) 821, 1118, 1126, 1423, 2147, 2431, 2626, 2865
MaeI CTAG 6 cut(s) 923, 1056, 1250, 1733, 2091, 2915
MaeII ACGT 7 cut(s) 17, 346, 365, 1985, 2212, 2242, 2929
MaeIII GTNAC 8 cut(s) 3, 209, 304, 356, 451, 566, 1981, 2615
MalI GATC 8 cut(s) 90, 552, 1535, 1612, 2107, 2350, 3079, 3171
MboI GATC 8 cut(s) 88, 550, 1533, 1610, 2105, 2348, 3077, 3169
MfeI CAATTG 3 cut(s) 1189, 2192, 3099
MflI RGATCY 3 cut(s) 1610, 2348, 3077
MhlI GDGCHC 2 cut(s) 1123, 2870
MluI ACGCGT 1 cut(s) 2578
MlyI GAGTC 6 cut(s) 1068, 1865, 2147, 2331, 2891, 3298
MmeI TCCRAC 2 cut(s) 1387, 3025
Mph1103I ATGCAT 3 cut(s) 1543, 2788, 3151
MroXI GAANNNNTTC 5 cut(s) 774, 1227, 1749, 2334, 2765
MslI CAYNNNNRTG 3 cut(s) 741, 999, 2062
MspA1I CMGCKG 2 cut(s) 1108, 3018
MspCI CTTAAG 3 cut(s) 1901, 2621, 2939
MspI CCGG 6 cut(s) 80, 198, 246, 1418, 3243, 3262
MspR9I CCNGG 7 cut(s) 54, 198, 199, 296, 1418, 2274, 2862
MunI CAATTG 3 cut(s) 1189, 2192, 3099
Mva1269I GAATGC 1 cut(s) 1620
MvaI CCWGG 4 cut(s) 54, 296, 2274, 2862
MvnI CGCG 2 cut(s) 2580, 3174
MwoI GCNNNNNNNGC 6 cut(s) 848, 1127, 1301, 2586, 2951, 3216
NciI CCSGG 3 cut(s) 198, 199, 1418
NcoI CCATGG 2 cut(s) 2566, 3048
NdeI CATATG 1 cut(s) 370
NdeII GATC 8 cut(s) 88, 550, 1533, 1610, 2105, 2348, 3077, 3169
NlaIV GGNNCC 6 cut(s) 401, 516, 642, 1024, 2128, 2433
NmuCI GTSAC 4 cut(s) 3, 209, 356, 451
NruI TCGCGA 1 cut(s) 3174
NsiI ATGCAT 3 cut(s) 1543, 2788, 3151
NspI RCATGY 2 cut(s) 740, 1545
NspV TTCGAA 1 cut(s) 2338
OliI CACNNNNGTG 1 cut(s) 999
PaeR7I CTCGAG 1 cut(s) 3520
PaqCI CACCTGC 1 cut(s) 1135
PciI ACATGT 1 cut(s) 736
PciSI GCTCTTC 1 cut(s) 3104
PcsI WCGNNNNNNNCGW 2 cut(s) 343, 1453
PctI GAATGC 1 cut(s) 1620
PdmI GAANNNNTTC 5 cut(s) 774, 1227, 1749, 2334, 2765
PfeI GAWTC 7 cut(s) 233, 299, 1643, 2036, 2640, 3128, 3358
PflMI CCANNNNNTGG 1 cut(s) 152
PfoI TCCNGGA 1 cut(s) 2272
PleI GAGTC 6 cut(s) 1067, 1864, 2147, 2330, 2890, 3297
PpsI GAGTC 6 cut(s) 1067, 1864, 2147, 2330, 2890, 3297
Ppu21I YACGTR 1 cut(s) 1986
PscI ACATGT 1 cut(s) 736
PshAI GACNNNNGTC 1 cut(s) 449
PshBI ATTAAT 2 cut(s) 1680, 2852
PsiI TTATAA 3 cut(s) 491, 806, 3257
Psp124BI GAGCTC 1 cut(s) 1123
Psp6I CCWGG 4 cut(s) 52, 294, 2272, 2860
PspFI CCCAGC 2 cut(s) 374, 1318
PspGI CCWGG 4 cut(s) 52, 294, 2272, 2860
PspN4I GGNNCC 6 cut(s) 401, 516, 642, 1024, 2128, 2433
PspPI GGNCC 5 cut(s) 200, 217, 641, 2126, 2188
PspXI VCTCGAGB 1 cut(s) 3520
PstI CTGCAG 4 cut(s) 1182, 1309, 2304, 2706
PstNI CAGNNNCTG 2 cut(s) 455, 2164
PsuI RGATCY 3 cut(s) 1610, 2348, 3077
PvuII CAGCTG 1 cut(s) 1108
RruI TCGCGA 1 cut(s) 3174
RsaI GTAC 7 cut(s) 401, 593, 1169, 1603, 2521, 2560, 3475
RsaNI GTAC 7 cut(s) 400, 592, 1168, 1602, 2520, 2559, 3474
RseI CAYNNNNRTG 3 cut(s) 741, 999, 2062
SacI GAGCTC 1 cut(s) 1123
SalI GTCGAC 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 3104
Sau3AI GATC 8 cut(s) 88, 550, 1533, 1610, 2105, 2348, 3077, 3169
Sau96I GGNCC 5 cut(s) 200, 217, 641, 2126, 2188
SchI GAGTC 6 cut(s) 1068, 1865, 2147, 2331, 2891, 3298
ScrFI CCNGG 7 cut(s) 54, 198, 199, 296, 1418, 2274, 2862
SduI GDGCHC 2 cut(s) 1123, 2870
SfcI CTRYAG 6 cut(s) 1178, 1305, 2118, 2300, 2702, 3178
Sfr274I CTCGAG 1 cut(s) 3520
SfuI TTCGAA 1 cut(s) 2338
SinI GGWCC 2 cut(s) 217, 641
SlaI CTCGAG 1 cut(s) 3520
SmaI CCCGGG 1 cut(s) 199
SmiMI CAYNNNNRTG 3 cut(s) 741, 999, 2062
SmlI CTYRAG 8 cut(s) 964, 1901, 2216, 2540, 2621, 2939, 2956, 3520
SmoI CTYRAG 8 cut(s) 964, 1901, 2216, 2540, 2621, 2939, 2956, 3520
SnaBI TACGTA 1 cut(s) 1986
SsiI CCGC 4 cut(s) 638, 867, 1597, 3016
SspI AATATT 4 cut(s) 438, 860, 903, 1474
SspMI CTAG 6 cut(s) 923, 1056, 1250, 1733, 2091, 2915
SstI GAGCTC 1 cut(s) 1123
StyD4I CCNGG 7 cut(s) 52, 196, 197, 294, 1416, 2272, 2860
StyI CCWWGG 3 cut(s) 1804, 2566, 3048
TaaI ACNGT 7 cut(s) 463, 1393, 1827, 1839, 3006, 3182, 3478
TaiI ACGT 7 cut(s) 20, 349, 368, 1988, 2215, 2245, 2932
TaqII GACCGA 2 cut(s) 3142, 3381
TatI WGTACW 2 cut(s) 591, 1167
TauI GCSGC 2 cut(s) 869, 1600
TfiI GAWTC 7 cut(s) 233, 299, 1643, 2036, 2640, 3128, 3358
TscAI CASTG 7 cut(s) 178, 268, 460, 477, 591, 651, 1078
TseFI GTSAC 4 cut(s) 3, 209, 356, 451
Tsp45I GTSAC 4 cut(s) 3, 209, 356, 451
TspGWI ACGGA 2 cut(s) 230, 2507
TspMI CCCGGG 1 cut(s) 197
TspRI CASTG 7 cut(s) 178, 268, 460, 477, 591, 651, 1078
Van91I CCANNNNNTGG 1 cut(s) 152
Vha464I CTTAAG 3 cut(s) 1901, 2621, 2939
VpaK11BI GGWCC 2 cut(s) 217, 641
VspI ATTAAT 2 cut(s) 1680, 2852
XagI CCTNNNNNAGG 1 cut(s) 2662
XapI RAATTY 6 cut(s) 1226, 1781, 2180, 2765, 3139, 3275
XbaI TCTAGA 2 cut(s) 1055, 1249
XceI RCATGY 2 cut(s) 740, 1545
XcmI CCANNNNNNNNNTGG 6 cut(s) 410, 417, 769, 1802, 3218, 3319
XhoI CTCGAG 1 cut(s) 3520
XmaI CCCGGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 443
XmnI GAANNNNTTC 5 cut(s) 774, 1227, 1749, 2334, 2765
XspI CTAG 6 cut(s) 923, 1056, 1250, 1733, 2091, 2915
Zsp2I ATGCAT 3 cut(s) 1543, 2788, 3151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.