MD16G1004100.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005277
Physical Location & Seq
Forward (+)
231947 .. 241755
9809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1004100.v1.1.491

Sequence Viewer

Length: 3489 bp
ATGTCGCAGAAAGCCAGGGCCCCACCTTCCTTCCAGTCAATCAAATCATTGCCCGGAGATTTTAGGTTCTCAGGTCTACCAGCTTCTGATCGATTTGGAAAATCCGATGATGGGAAATTGGGAAATAGCAATGTCATCTCCTCGAGTATTCCAGAAAATGGTGGTTTTGAGGGGGTTGAAGGGAGTAGCGGGCCTTTTGGCGACTTGGAACAGGTAGACGATTCACCATATGGCAGGAACACAATCTCCATCGAAGACAGGCCCTTAAGGGGTGATGAAGACTTGGATTATGTTGCTGCATCTGTGCCTTCAATATCTTCATCCCATAGGGAAAGTGGGTGGGGTGACACCAATCCTTATGCTGTGAAGAAGCTTCAATCTTGGTTCCAACTGCCAAATGGGAATTGGGAACTGGGGAGAATATTGTCGACCTCAGGCGCTGAATCTGTTATATCACTCCCTAACGATAAAGTTGTAACAGTAAAAACTGAAGATTTGGTTTCAGCTAATCCAGATATCCTTGATGGTGTGGATGATCTCATGCAACTTAGTTACCTAAATGAGCCATCAGTGTTGTACAATCTTCAATATAGATACAATCAAGATATGATTTATACAAAAGCGGGACCAGTGTTGGTTGCTGTCAATCCCTTTAAGAGAGTTTCTTTATATGGGAATGAATACATTGAAGCATACAAGCGTAAAGCTGTTGAAAATCCACATGTGTATGCCATTGCAGACACAGCCATTCGGGAAATGGTTCGAGATGAAGTAAATCAGTCTATAATCATAAGTGGGGAAAGTGGAGCAGGAAAAACTGAAACTGCAAAGATAGCAATGCAATATCTGGCTGCACTTGGAGGTGGAAGTGGAATAGAGCATGAAATTTTGAAAACTAATCCTATACTGGAAGCCTTTGGTAATGCAAAAACATTGAGAAATGACAACTCAAGTCGCTTTGGAAAATTGATTGAAATCCACTTCAGTGAAACTGGAAAAATTTCTGGTGCCAATATTCAAACTTTTTTGCTTGAAAAGTCTAGAGTGGTCCAATGCACTGAAGGAGAAAGGTCCTATCATATATTTTATCAGCTTTGTGCTGGAGCTCCACCTGCTCTTAGAGAGATGCTGAACTTGAAGAGTGCAGATGAGTATCAATATCTGAAGCAAAGCAATTGTTATTCCATTACTGGGGTTAATGATGCTGAAGAATTCAGTGTGGTGAAGGAAGCTCTAGATGTTGTCCATATAAATGAAGAAGACCAACAAAGTGTATTTGCAATGCTTGCTGCAGTTTTGTGGCTGGGAAACATCTCATTTAGTGTGATTGATAACGAAAATCATGTTGAACCTGTAGAAGATGAAGGTCTGTTCAATGTCGCAAAATTGATTGGATGTGGTGTGGATGAACTTAAGCTTGCTTTATCGACTCGAAAAATGAGAGTTGGTAATGACAGTATCGTCCAGAAGCTAACGCTATCACAGGCCATCGACACAAGAGATGCTTTGTCAAAGTCAATTTATGCATGTTTGTTTGAGTGGTTGGTTGAACAAATTAACAAATCACTTGCAGTAGGAAAACGGCGTACTGGCAGATCTATCAGCATTCTTGATATCTATGGTTTTGAATCATTTGATAGAAATAGTTTTGAACAGTTCTGCATTAATTATGCAAATGAGAGATTGCAACAACACTTCAATCGTCACTTATTCAAGTTAGAGCAGGATGAATATATCCAAGACGGAATTGATTGGACAAAAGTTGAATTTGAAGACAACCAAGATTGTCTTGGTCTTTTCGAAAAGAGACCGTTGGGATTACTGTCTCTGTTAGATGAGGAGTCCACCTTCCCAAATGGTACTGATTTGACCTTTGCCAACAAGCTCAAGCAACATCTGAGCTCCAATTCTTGTTTCAAAGGAGAACGAGGCAAATTGTTTGCTGTCAGTCATTATGCAGGAGAGGTTTCATATGACACAACAGGATTTTTGGAAAAGAACAGGGACTTGCTGCATTTAGATTCTATTCAGCTTCTTTCCTCATGCTCATGCCACCTTCCTCAGATATTTGCATCTAGTATGCTTAAAGTAGGTGGTGGAGTAGACTCCCAGAAGCTGAGTGTTGCAACAAAATTTAAGAGTCAATTGTTCCTATTGATGAAACGTCTAGAAAACACCACTCCACATTTCATACGTTGTATCAAGCCCAACAATCTTCAATCCCCTGGACTCTATGAGCAAGGACTTGTACTGCAGCAGCTGAGATGTTGTGGAGTTCTAGAAGTGGTTCGAATATCAAGATCTGGCTTTCCTACCAGAATGTCACACCAGAAGTTTGCAAGAAGATATGGTTTTCTTCTGCTGGAGAATGTTGCATCTCAGGATCCACTTAGTGTTTCAGTTGCTATTCTTCATCAGTTCAACATATTACCAGAGATGTATCAAGTGGGCTATACAAAGTTATTTTTCCGAACTGGACAGATTGGGGTGCTTGAGGACACCAGAAACCGTACGCTCCATGGTATTTTACGTGTTCAAAGCTGCTTTAGAGGACACCAAGCTCGGTGTTACCTCAAGGAGCTGAGGAGAGGAATCACCACTCTGCAGTCATTTATCAGGGGACAGAAAACCAGGAAGGAGTACTCAATATTACTAGAGAGGCATAGAGCTGCTGTTGTCATACAAAAGCAGGTGAAAAGCAGGTTTGCAAGGAAGAAATTCAACAACATTTATGATGCATCAATCGTGGTACAATCAGTTCTTCGTGGCTGGTTGGTCAGAAGATGCTCAGGAAGTATAGGATTGTTGAAGCCCGGAAGCACGAAGGCTAACGAGTCAGATGATGTGCTGGTAAAGGCATCTTTCCTTGCTGAATTACAGCGCCGCGTTCTTAAGGCTGAAGCTGCCCTGCGAGAGAAGGAAGAGGAGAATGACATCCTCCACCAACGTCTCCAACAGTATGAGAGCCGCTGGTCGGAATATGAGTTGAAAATGAAATCCATGGAAGAAGTGTGGCAGAAACAAATGAGATCCCTACAATCCAGCCTCTCAATTGCAAAGAAGAGCTTAGCCATTGATGATTCCGAGAGAAATTCTGATGCATCAGTGAATGCAAGTGATGACCGTGAGTACGGTTGGGATACAGGAAGTAACCATAGAGGCCAAGATAGCAATGGGGTGAGACCAATGAGTGCAGGTTTGAGCGTCATAAGTCGGTTAACTGAAGAGTTTGATCAGAGGAGTCAAGTTTTTGGCGATGATGCCAAGTTCTTGGTGGAGGTAAAGTCTGGTCAAGTGGAAGCGAGTCTGAATCCAGACCGAGAGCTAAGAAGGTTGAAGCAGATGTTTGAGGCTTGGAAGAAGGATTATGGGACAAGACTAAGGGAGACAAAGGTGATTTTGCATAAGATTGGAAATGAAGAGGGAGGCAGTGCTGACAGGGTGAAAAAGAAGTGGTGGGGAAGGCGGAACAGCTCAAGGATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_head

Protein Analysis

1163

Amino Acids

130.88

Weight (kDa)

7.08

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_VIII-1_N PF25369 125 - 169 9.3e-25 Myosin-1-like, N-terminal SH3 domain
Myosin_head PF00063 177 - 824 5.8e-241 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1120, 2680
AasI GACNNNNNNGTC 1 cut(s) 1460
Acc36I ACCTGC 4 cut(s) 1120, 2680, 2691, 3185
AccB1I GGYRCC 1 cut(s) 1007
AccB7I CCANNNNNTGG 1 cut(s) 158
AccI GTMKAC 4 cut(s) 76, 216, 428, 2103
AccII CGCG 1 cut(s) 2886
AciI CCGC 5 cut(s) 189, 623, 2884, 2968, 3466
AclWI GGATC 3 cut(s) 2378, 2391, 3024
AcsI RAATTY 7 cut(s) 885, 999, 1211, 1766, 2132, 2717, 3091
AcuI CTGAAG 7 cut(s) 510, 967, 1080, 1184, 1227, 2919, 3243
AdeI CACNNNGTG 1 cut(s) 2393
AfaI GTAC 8 cut(s) 578, 1588, 1861, 2250, 2512, 2642, 2751, 3131
AfiI CCNNNNNNNGG 5 cut(s) 111, 158, 269, 1191, 2974
AflII CTTAAG 3 cut(s) 265, 1412, 2891
AflIII ACRYGT 2 cut(s) 721, 2530
AjnI CCWGG 3 cut(s) 14, 2224, 2630
AjuI GAANNNNNNNTTGG 2 cut(s) 1898, 1930
AleI CACNNNNGTG 1 cut(s) 984
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
Alw21I GWGCWC 2 cut(s) 1108, 1904
Alw26I GTCTC 5 cut(s) 1801, 1830, 2954, 3175, 3380
AlwI GGATC 3 cut(s) 2378, 2391, 3024
AlwNI CAGNNNCTG 4 cut(s) 86, 440, 2116, 2260
Ama87I CYCGRG 1 cut(s) 142
AoxI GGCC 5 cut(s) 18, 191, 260, 1485, 3160
ApaI GGGCCC 1 cut(s) 22
ApeKI GCWGC 9 cut(s) 296, 851, 1289, 2011, 2254, 2257, 2541, 2669, 2903
ApoI RAATTY 7 cut(s) 885, 999, 1211, 1766, 2132, 2717, 3091
AseI ATTAAT 2 cut(s) 1665, 3483
Asp700I GAANNNNTTC 4 cut(s) 759, 1000, 2286, 2717
AspLEI GCGC 2 cut(s) 440, 2883
AspS9I GGNCC 7 cut(s) 18, 19, 191, 261, 626, 1048, 1071
AsuC2I CCSGG 2 cut(s) 54, 2814
AsuHPI GGTGA 9 cut(s) 216, 284, 356, 1234, 2587, 2704, 3190, 3406, 3454
AsuII TTCGAA 2 cut(s) 1800, 2290
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 3 cut(s) 626, 1048, 1071
AxyI CCTNAGG 1 cut(s) 433
BaeGI GKGCMC 1 cut(s) 22
BamHI GGATCC 1 cut(s) 2383
BanI GGYRCC 1 cut(s) 1007
BanII GRGCYC 3 cut(s) 22, 1108, 1904
BbsI GAAGAC 4 cut(s) 261, 285, 1266, 1779
Bbv12I GWGCWC 2 cut(s) 1108, 1904
BbvCI CCTCAGC 1 cut(s) 2582
BbvI GCAGC 9 cut(s) 283, 838, 1276, 1998, 2266, 2269, 2528, 2656, 2890
BccI CCATC 5 cut(s) 104, 257, 518, 574, 1496
BceAI ACGGC 1 cut(s) 1598
BciT130I CCWGG 3 cut(s) 16, 2226, 2632
BciVI GTATCC 1 cut(s) 3133
BclI TGATCA 1 cut(s) 3232
BcnI CCSGG 2 cut(s) 54, 2814
BcoDI GTCTC 5 cut(s) 1801, 1830, 2954, 3175, 3380
BfaI CTAG 6 cut(s) 1041, 1235, 2076, 2168, 2279, 2654
BfmI CTRYAG 4 cut(s) 1290, 1353, 2252, 2603
BfoI RGCGCY 2 cut(s) 441, 2884
BfrI CTTAAG 3 cut(s) 265, 1412, 2891
BfuAI ACCTGC 4 cut(s) 1120, 2680, 2691, 3185
BfuI GTATCC 1 cut(s) 3133
BglII AGATCT 2 cut(s) 1595, 2300
BlpI GCTNAGC 1 cut(s) 3067
BmcAI AGTACT 1 cut(s) 2642
Bme1390I CCNGG 5 cut(s) 16, 54, 2226, 2632, 2814
Bme18I GGWCC 3 cut(s) 626, 1048, 1071
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 7 cut(s) 18, 19, 191, 261, 626, 1048, 1071
BmiI GGNNCC 6 cut(s) 20, 21, 386, 627, 1009, 2385
BmrFI CCNGG 5 cut(s) 16, 54, 2226, 2632, 2814
BmrI ACTGGG 2 cut(s) 422, 1200
BmuI ACTGGG 2 cut(s) 422, 1200
BpiI GAAGAC 4 cut(s) 261, 285, 1266, 1779
BpmI CTGGAG 2 cut(s) 1122, 2384
Bpu10I CCTNAGC 2 cut(s) 2582, 2788
Bpu1102I GCTNAGC 1 cut(s) 3067
Bpu14I TTCGAA 2 cut(s) 1800, 2290
BpuEI CTTGAG 5 cut(s) 934, 1871, 2513, 2558, 3460
BpuMI CCSGG 2 cut(s) 54, 2814
Bsa29I ATCGAT 1 cut(s) 91
BsaAI YACGTR 1 cut(s) 2531
BsaBI GATNNNNATC 2 cut(s) 974, 1152
BsaI GGTCTC 2 cut(s) 1801, 3175
BsaJI CCNNGG 4 cut(s) 15, 2224, 2518, 3000
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 3269, 3299
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 158, 269, 1191, 2974
Bse1I ACTGG 8 cut(s) 34, 417, 629, 912, 997, 1195, 1594, 2479
Bse21I CCTNAGG 1 cut(s) 433
Bse3DI GCAATG 6 cut(s) 47, 136, 732, 843, 1287, 3178
Bse8I GATNNNNATC 2 cut(s) 974, 1152
BseBI CCWGG 3 cut(s) 16, 2226, 2632
BseCI ATCGAT 1 cut(s) 91
BseDI CCNNGG 4 cut(s) 15, 2224, 2518, 3000
BseGI GGATG 6 cut(s) 320, 538, 1400, 1411, 1732, 2934
BseJI GATNNNNATC 2 cut(s) 974, 1152
BseLI CCNNNNNNNGG 5 cut(s) 111, 158, 269, 1191, 2974
BseMI GCAATG 6 cut(s) 47, 136, 732, 843, 1287, 3178
BseMII CTCAG 9 cut(s) 84, 447, 1889, 2075, 2108, 2252, 2393, 2573, 2802
BseNI ACTGG 8 cut(s) 34, 417, 629, 912, 997, 1195, 1594, 2479
BseRI GAGGAG 5 cut(s) 130, 1853, 2599, 2939, 3253
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 9 cut(s) 283, 838, 1276, 1998, 2266, 2269, 2528, 2656, 2890
BseYI CCCAGC 1 cut(s) 1303
BsgI GTGCAG 3 cut(s) 837, 1164, 3213
Bsh1236I CGCG 1 cut(s) 2886
BshFI GGCC 5 cut(s) 20, 193, 262, 1487, 3162
BshNI GGYRCC 1 cut(s) 1007
BshVI ATCGAT 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 1108, 1904
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 2 cut(s) 54, 2814
BsiWI CGTACG 1 cut(s) 2510
BslFI GGGAC 4 cut(s) 639, 2018, 2634, 3385
BslI CCNNNNNNNGG 5 cut(s) 111, 158, 269, 1191, 2974
BsmAI GTCTC 5 cut(s) 1801, 1830, 2954, 3175, 3380
BsmBI CGTCTC 1 cut(s) 2954
BsmFI GGGAC 4 cut(s) 639, 2018, 2634, 3385
BsmI GAATGC 2 cut(s) 1605, 3115
BsnI GGCC 5 cut(s) 20, 193, 262, 1487, 3162
Bso31I GGTCTC 2 cut(s) 1801, 3175
BsoBI CYCGRG 1 cut(s) 142
Bsp119I TTCGAA 2 cut(s) 1800, 2290
Bsp120I GGGCCC 1 cut(s) 18
Bsp1286I GDGCHC 3 cut(s) 22, 1108, 1904
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 7 cut(s) 88, 535, 1595, 2300, 2383, 3029, 3232
Bsp1720I GCTNAGC 1 cut(s) 3067
Bsp19I CCATGG 2 cut(s) 2518, 3000
BspACI CCGC 5 cut(s) 189, 623, 2884, 2968, 3466
BspANI GGCC 5 cut(s) 20, 193, 262, 1487, 3162
BspCNI CTCAG 9 cut(s) 83, 446, 1890, 2074, 2109, 2253, 2392, 2574, 2801
BspDI ATCGAT 1 cut(s) 91
BspFNI CGCG 1 cut(s) 2886
BspLI GGNNCC 6 cut(s) 20, 21, 386, 627, 1009, 2385
BspMAI CTGCAG 3 cut(s) 1294, 2256, 2607
BspMI ACCTGC 4 cut(s) 1120, 2680, 2691, 3185
BspPI GGATC 3 cut(s) 2378, 2391, 3024
BspQI GCTCTTC 1 cut(s) 3056
BspT104I TTCGAA 2 cut(s) 1800, 2290
BspT107I GGYRCC 1 cut(s) 1007
BspTI CTTAAG 3 cut(s) 265, 1412, 2891
BspTNI GGTCTC 2 cut(s) 1801, 3175
BsrDI GCAATG 6 cut(s) 47, 136, 732, 843, 1287, 3178
BsrGI TGTACA 1 cut(s) 576
BsrI ACTGG 8 cut(s) 34, 417, 629, 912, 997, 1195, 1594, 2479
BssECI CCNNGG 4 cut(s) 15, 2224, 2518, 3000
BssMI GATC 7 cut(s) 88, 535, 1595, 2300, 2383, 3029, 3232
BssT1I CCWWGG 2 cut(s) 2518, 3000
Bst2UI CCWGG 3 cut(s) 16, 2226, 2632
Bst4CI ACNGT 9 cut(s) 481, 1457, 1656, 1812, 1824, 2510, 2958, 3125, 3134
Bst6I CTCTTC 5 cut(s) 1133, 2916, 3056, 3219, 3414
BstAFI CTTAAG 3 cut(s) 265, 1412, 2891
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstAUI TGTACA 1 cut(s) 576
BstBAI YACGTR 1 cut(s) 2531
BstBI TTCGAA 2 cut(s) 1800, 2290
BstC8I GCNNGC 3 cut(s) 191, 1287, 1419
BstDSI CCRYGG 2 cut(s) 2518, 3000
BstF5I GGATG 6 cut(s) 320, 538, 1400, 1411, 1732, 2934
BstFNI CGCG 1 cut(s) 2886
BstH2I RGCGCY 2 cut(s) 441, 2884
BstHHI GCGC 2 cut(s) 440, 2883
BstKTI GATC 7 cut(s) 91, 538, 1598, 2303, 2386, 3032, 3235
BstMAI GTCTC 5 cut(s) 1801, 1830, 2954, 3175, 3380
BstMBI GATC 7 cut(s) 88, 535, 1595, 2300, 2383, 3029, 3232
BstMWI GCNNNNNNNGC 6 cut(s) 743, 833, 1112, 1286, 2903, 3168
BstNI CCWGG 3 cut(s) 16, 2226, 2632
BstNSI RCATGY 2 cut(s) 725, 1530
BstSCI CCNGG 5 cut(s) 14, 52, 2224, 2630, 2812
BstSFI CTRYAG 4 cut(s) 1290, 1353, 2252, 2603
BstSLI GKGCMC 1 cut(s) 22
BstUI CGCG 1 cut(s) 2886
BstV1I GCAGC 9 cut(s) 283, 838, 1276, 1998, 2266, 2269, 2528, 2656, 2890
BstV2I GAAGAC 4 cut(s) 261, 285, 1266, 1779
BstX2I RGATCY 4 cut(s) 1595, 2300, 2383, 3029
BstXI CCANNNNNNTGG 1 cut(s) 3271
BstYI RGATCY 4 cut(s) 1595, 2300, 2383, 3029
Bsu15I ATCGAT 1 cut(s) 91
Bsu36I CCTNAGG 1 cut(s) 433
BsuI GTATCC 1 cut(s) 3133
BsuRI GGCC 5 cut(s) 20, 193, 262, 1487, 3162
BsuTUI ATCGAT 1 cut(s) 91
BtgI CCRYGG 2 cut(s) 2518, 3000
BtgZI GCGATG 1 cut(s) 3270
BtsCI GGATG 6 cut(s) 320, 538, 1400, 1411, 1732, 2934
BtsI GCAGTG 1 cut(s) 3436
BtsIMutI CAGTG 7 cut(s) 576, 636, 991, 1056, 1222, 3111, 3436
BveI ACCTGC 4 cut(s) 1120, 2680, 2691, 3185
Cac8I GCNNGC 3 cut(s) 191, 1287, 1419
CaiI CAGNNNCTG 4 cut(s) 86, 440, 2116, 2260
CfoI GCGC 2 cut(s) 440, 2883
Cfr13I GGNCC 7 cut(s) 18, 19, 191, 261, 626, 1048, 1071
ClaI ATCGAT 1 cut(s) 91
CseI GACGC 1 cut(s) 3193
Csp6I GTAC 8 cut(s) 577, 1587, 1860, 2249, 2511, 2641, 2750, 3130
CviAII CATG 9 cut(s) 541, 722, 881, 1343, 1527, 2043, 2049, 2519, 3001
CviQI GTAC 8 cut(s) 577, 1587, 1860, 2249, 2511, 2641, 2750, 3130
DpnI GATC 7 cut(s) 90, 537, 1597, 2302, 2385, 3031, 3234
DpnII GATC 7 cut(s) 88, 535, 1595, 2300, 2383, 3029, 3232
DraIII CACNNNGTG 1 cut(s) 2393
DrdI GACNNNNNNGTC 1 cut(s) 1460
DseDI GACNNNNNNGTC 1 cut(s) 1460
Eam1104I CTCTTC 5 cut(s) 1133, 2916, 3056, 3219, 3414
EarI CTCTTC 5 cut(s) 1133, 2916, 3056, 3219, 3414
EciI GGCGGA 1 cut(s) 3481
Ecl136II GAGCTC 2 cut(s) 1106, 1902
Eco130I CCWWGG 2 cut(s) 2518, 3000
Eco24I GRGCYC 3 cut(s) 22, 1108, 1904
Eco31I GGTCTC 2 cut(s) 1801, 3175
Eco32I GATATC 2 cut(s) 517, 1615
Eco47I GGWCC 3 cut(s) 626, 1048, 1071
Eco53kI GAGCTC 2 cut(s) 1106, 1902
Eco57I CTGAAG 7 cut(s) 510, 967, 1080, 1184, 1227, 2919, 3243
Eco81I CCTNAGG 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 142
EcoICRI GAGCTC 2 cut(s) 1106, 1902
EcoO109I RGGNCCY 4 cut(s) 18, 19, 261, 1071
EcoRI GAATTC 1 cut(s) 1211
EcoRII CCWGG 3 cut(s) 14, 2224, 2630
EcoRV GATATC 2 cut(s) 517, 1615
EcoT14I CCWWGG 2 cut(s) 2518, 3000
EcoT22I ATGCAT 3 cut(s) 1528, 2740, 3103
EcoT38I GRGCYC 3 cut(s) 22, 1108, 1904
ErhI CCWWGG 2 cut(s) 2518, 3000
Esp3I CGTCTC 1 cut(s) 2954
FaeI CATG 9 cut(s) 544, 725, 884, 1346, 1530, 2046, 2052, 2522, 3004
FalI AAGNNNNNCTT 6 cut(s) 1489, 1521, 1773, 1805, 3050, 3082
FaqI GGGAC 4 cut(s) 639, 2018, 2634, 3385
FatI CATG 9 cut(s) 540, 721, 880, 1342, 1526, 2042, 2048, 2518, 3000
FauI CCCGC 2 cut(s) 182, 616
FauNDI CATATG 2 cut(s) 229, 1972
FbaI TGATCA 1 cut(s) 3232
FblI GTMKAC 4 cut(s) 76, 216, 428, 2103
FokI GGATG 6 cut(s) 307, 545, 1407, 1418, 1739, 2921
FriOI GRGCYC 3 cut(s) 22, 1108, 1904
FspBI CTAG 6 cut(s) 1041, 1235, 2076, 2168, 2279, 2654
GlaI GCGC 2 cut(s) 439, 2882
GsaI CCCAGC 1 cut(s) 1307
GsuI CTGGAG 2 cut(s) 1122, 2384
HaeII RGCGCY 2 cut(s) 441, 2884
HaeIII GGCC 5 cut(s) 20, 193, 262, 1487, 3162
HapII CCGG 2 cut(s) 54, 2814
HgaI GACGC 1 cut(s) 3193
HhaI GCGC 2 cut(s) 440, 2883
Hin1II CATG 9 cut(s) 544, 725, 884, 1346, 1530, 2046, 2052, 2522, 3004
Hin6I GCGC 2 cut(s) 438, 2881
HinP1I GCGC 2 cut(s) 438, 2881
HincII GTYRAC 2 cut(s) 429, 3219
HindII GTYRAC 2 cut(s) 429, 3219
HindIII AAGCTT 2 cut(s) 371, 1415
HpaI GTTAAC 1 cut(s) 3219
HpaII CCGG 2 cut(s) 54, 2814
HphI GGTGA 9 cut(s) 216, 284, 356, 1234, 2587, 2704, 3190, 3406, 3454
Hpy166II GTNNAC 6 cut(s) 77, 217, 429, 1845, 2104, 3219
Hpy8I GTNNAC 6 cut(s) 77, 217, 429, 1845, 2104, 3219
HpyCH4III ACNGT 9 cut(s) 481, 1457, 1656, 1812, 1824, 2510, 2958, 3125, 3134
HpyCH4IV ACGT 4 cut(s) 2164, 2194, 2530, 2947
HpyF10VI GCNNNNNNNGC 6 cut(s) 743, 833, 1112, 1286, 2903, 3168
HpySE526I ACGT 4 cut(s) 2164, 2194, 2530, 2947
Hsp92II CATG 9 cut(s) 544, 725, 884, 1346, 1530, 2046, 2052, 2522, 3004
HspAI GCGC 2 cut(s) 438, 2881
Ksp22I TGATCA 1 cut(s) 3232
KspAI GTTAAC 1 cut(s) 3219
Kzo9I GATC 7 cut(s) 88, 535, 1595, 2300, 2383, 3029, 3232
LguI GCTCTTC 1 cut(s) 3056
LmnI GCTCC 6 cut(s) 806, 1103, 1111, 1907, 2520, 2578
Lsp1109I GCAGC 9 cut(s) 283, 838, 1276, 1998, 2266, 2269, 2528, 2656, 2890
MaeI CTAG 6 cut(s) 1041, 1235, 2076, 2168, 2279, 2654
MaeII ACGT 4 cut(s) 2164, 2194, 2530, 2947
MaeIII GTNAC 7 cut(s) 344, 475, 551, 1703, 2322, 2567, 3149
MalI GATC 7 cut(s) 90, 537, 1597, 2302, 2385, 3031, 3234
MboI GATC 7 cut(s) 88, 535, 1595, 2300, 2383, 3029, 3232
MfeI CAATTG 3 cut(s) 1174, 2144, 3051
MflI RGATCY 4 cut(s) 1595, 2300, 2383, 3029
MhlI GDGCHC 3 cut(s) 22, 1108, 1904
MlyI GAGTC 8 cut(s) 1423, 1850, 2099, 2149, 2223, 2843, 3250, 3313
MmeI TCCRAC 3 cut(s) 412, 2955, 2977
Mph1103I ATGCAT 3 cut(s) 1528, 2740, 3103
MroXI GAANNNNTTC 4 cut(s) 759, 1000, 2286, 2717
MslI CAYNNNNRTG 4 cut(s) 726, 984, 1251, 2047
MspA1I CMGCKG 2 cut(s) 2260, 2970
MspCI CTTAAG 3 cut(s) 265, 1412, 2891
MspI CCGG 2 cut(s) 54, 2814
MspR9I CCNGG 5 cut(s) 16, 54, 2226, 2632, 2814
MunI CAATTG 3 cut(s) 1174, 2144, 3051
Mva1269I GAATGC 2 cut(s) 1605, 3115
MvaI CCWGG 3 cut(s) 16, 2226, 2632
MvnI CGCG 1 cut(s) 2886
MwoI GCNNNNNNNGC 6 cut(s) 743, 833, 1112, 1286, 2903, 3168
NciI CCSGG 2 cut(s) 54, 2814
NcoI CCATGG 2 cut(s) 2518, 3000
NdeI CATATG 2 cut(s) 229, 1972
NdeII GATC 7 cut(s) 88, 535, 1595, 2300, 2383, 3029, 3232
NlaIII CATG 9 cut(s) 544, 725, 884, 1346, 1530, 2046, 2052, 2522, 3004
NlaIV GGNNCC 6 cut(s) 20, 21, 386, 627, 1009, 2385
NmuCI GTSAC 3 cut(s) 344, 1703, 2322
NsiI ATGCAT 3 cut(s) 1528, 2740, 3103
NspI RCATGY 2 cut(s) 725, 1530
NspV TTCGAA 2 cut(s) 1800, 2290
OliI CACNNNNGTG 1 cut(s) 984
PacI TTAATTAA 1 cut(s) 3487
PaeR7I CTCGAG 1 cut(s) 142
PaqCI CACCTGC 2 cut(s) 1120, 2680
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 3056
PctI GAATGC 2 cut(s) 1605, 3115
PdmI GAANNNNTTC 4 cut(s) 759, 1000, 2286, 2717
PfeI GAWTC 7 cut(s) 221, 443, 1628, 2021, 2592, 3080, 3310
Pfl23II CGTACG 1 cut(s) 2510
PflMI CCANNNNNTGG 1 cut(s) 158
PleI GAGTC 8 cut(s) 1423, 1849, 2099, 2148, 2223, 2842, 3249, 3312
PpsI GAGTC 8 cut(s) 1423, 1849, 2099, 2148, 2223, 2842, 3249, 3312
Ppu21I YACGTR 1 cut(s) 2531
PpuMI RGGWCCY 1 cut(s) 1071
PscI ACATGT 1 cut(s) 721
PshBI ATTAAT 2 cut(s) 1665, 3483
Psp124BI GAGCTC 2 cut(s) 1108, 1904
Psp5II RGGWCCY 1 cut(s) 1071
Psp6I CCWGG 3 cut(s) 14, 2224, 2630
PspFI CCCAGC 1 cut(s) 1303
PspGI CCWGG 3 cut(s) 14, 2224, 2630
PspLI CGTACG 1 cut(s) 2510
PspN4I GGNNCC 6 cut(s) 20, 21, 386, 627, 1009, 2385
PspOMI GGGCCC 1 cut(s) 18
PspPI GGNCC 7 cut(s) 18, 19, 191, 261, 626, 1048, 1071
PspPPI RGGWCCY 1 cut(s) 1071
PspXI VCTCGAGB 1 cut(s) 142
PsrI GAACNNNNNNTAC 2 cut(s) 2742, 2774
PstI CTGCAG 3 cut(s) 1294, 2256, 2607
PstNI CAGNNNCTG 4 cut(s) 86, 440, 2116, 2260
PsuI RGATCY 4 cut(s) 1595, 2300, 2383, 3029
PvuII CAGCTG 1 cut(s) 2260
RsaI GTAC 8 cut(s) 578, 1588, 1861, 2250, 2512, 2642, 2751, 3131
RsaNI GTAC 8 cut(s) 577, 1587, 1860, 2249, 2511, 2641, 2750, 3130
RseI CAYNNNNRTG 4 cut(s) 726, 984, 1251, 2047
SacI GAGCTC 2 cut(s) 1108, 1904
SalI GTCGAC 1 cut(s) 427
SapI GCTCTTC 1 cut(s) 3056
Sau3AI GATC 7 cut(s) 88, 535, 1595, 2300, 2383, 3029, 3232
Sau96I GGNCC 7 cut(s) 18, 19, 191, 261, 626, 1048, 1071
ScaI AGTACT 1 cut(s) 2642
SchI GAGTC 8 cut(s) 1423, 1850, 2099, 2149, 2223, 2843, 3250, 3313
ScrFI CCNGG 5 cut(s) 16, 54, 2226, 2632, 2814
SduI GDGCHC 3 cut(s) 22, 1108, 1904
SfcI CTRYAG 4 cut(s) 1290, 1353, 2252, 2603
Sfr274I CTCGAG 1 cut(s) 142
SfuI TTCGAA 2 cut(s) 1800, 2290
SinI GGWCC 3 cut(s) 626, 1048, 1071
SlaI CTCGAG 1 cut(s) 142
SmiMI CAYNNNNRTG 4 cut(s) 726, 984, 1251, 2047
SmlI CTYRAG 9 cut(s) 142, 265, 949, 1412, 1886, 2492, 2573, 2891, 3475
SmoI CTYRAG 9 cut(s) 142, 265, 949, 1412, 1886, 2492, 2573, 2891, 3475
SsiI CCGC 5 cut(s) 189, 623, 2884, 2968, 3466
SspI AATATT 3 cut(s) 423, 1015, 2649
SspMI CTAG 6 cut(s) 1041, 1235, 2076, 2168, 2279, 2654
SstI GAGCTC 2 cut(s) 1108, 1904
StyD4I CCNGG 5 cut(s) 14, 52, 2224, 2630, 2812
StyI CCWWGG 2 cut(s) 2518, 3000
TaaI ACNGT 9 cut(s) 481, 1457, 1656, 1812, 1824, 2510, 2958, 3125, 3134
TaiI ACGT 4 cut(s) 2167, 2197, 2533, 2950
TaqII GACCGA 1 cut(s) 3333
TatI WGTACW 3 cut(s) 576, 2248, 2640
TauI GCSGC 2 cut(s) 2886, 2970
TfiI GAWTC 7 cut(s) 221, 443, 1628, 2021, 2592, 3080, 3310
TscAI CASTG 7 cut(s) 576, 636, 991, 1063, 1222, 3111, 3436
TseFI GTSAC 3 cut(s) 344, 1703, 2322
TseI GCWGC 9 cut(s) 296, 851, 1289, 2011, 2254, 2257, 2541, 2669, 2903
Tsp45I GTSAC 3 cut(s) 344, 1703, 2322
TspGWI ACGGA 1 cut(s) 1758
TspRI CASTG 7 cut(s) 576, 636, 991, 1063, 1222, 3111, 3436
Van91I CCANNNNNTGG 1 cut(s) 158
Vha464I CTTAAG 3 cut(s) 265, 1412, 2891
VpaK11BI GGWCC 3 cut(s) 626, 1048, 1071
VspI ATTAAT 2 cut(s) 1665, 3483
XapI RAATTY 7 cut(s) 885, 999, 1211, 1766, 2132, 2717, 3091
XbaI TCTAGA 4 cut(s) 1040, 1234, 2167, 2278
XceI RCATGY 2 cut(s) 725, 1530
XcmI CCANNNNNNNNNTGG 7 cut(s) 332, 395, 402, 754, 1787, 3170, 3271
XhoI CTCGAG 1 cut(s) 142
XmiI GTMKAC 4 cut(s) 76, 216, 428, 2103
XmnI GAANNNNTTC 4 cut(s) 759, 1000, 2286, 2717
XspI CTAG 6 cut(s) 1041, 1235, 2076, 2168, 2279, 2654
ZrmI AGTACT 1 cut(s) 2642
Zsp2I ATGCAT 3 cut(s) 1528, 2740, 3103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.