Rh4CG463800
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005277
Physical Location & Seq
Forward (+)
69398947 .. 69413914
14968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG463800.1

Sequence Viewer

Length: 3540 bp
ATGAAAAAAGCAGGGCATTTGACGATTGCTTCCAGCAAGCAAAAATCTCGGAGGCGGATTCACATTTTCTCTGAGGTTGCAAAAGCTCTTTGGGGAAAATGTCACAGAAATCTAGGTCCACACCTTCCTTCCAGTCAATCAAATCATTGCCTGGCGATTTTAGAAAATGGTGAACTAAAGGATAGCATTGCTGAAGGTAGTCCCGGGCCTCTTGGTGACATGGACCAAGTAGTTGATGATTCACCATATACCGGCAACACTGTCTCCACTGAAGACAGGCAGTCAAGTGTTGATGAGGACCTGGATTCTGTAACTCCACCTTTACCCTCAATATCTTCATTTCGCACAGAACGTCGGTGGGGTGACACCACGTCATATGCTGGGAAGAAGAAGCTACAATCTTGGTACCAGCTTCCAAATGGGAATTGGGAACTGGGGAAAATATTGTCAACTTCAGTCACTGAAACTGTTATATCACTGCCTAACGAGAAGGTTATAAAGGTAAAAACTGAAGATTTGGTTCCAGCAAATCCTGATATCCTTGATGGTGTGGATGATCTCATGCAACTTAGTTACTTAAACGAACCATCAGTGCTGTACAATCTTCAGTATAGATACAATCGAGATATGATTTATACAAAAGCGGGACCAGTGTTGGTTGCTATCAACCCCTTTAAGAAAGTTTGTTTATATGGGAATGAATACATTGAAGCATATAAGCGTAAAGCTGTTGATAGTCCACATGTATATGCCATCACAGACACAGCCATCCGAGAAATGGTTCGAGATGAAGTAAATCAATCGATAATTATAAGTGGGGAAAGTGGAGCAGGAAAAACTGAAACTGCAAAGATAGCAATGCAATATTTGGCTGCACTTGGAGGTGGAAGTGGAATAGAACATGAAATATTGAAAACTAATCCGATACTAGAAGCCTTTGGTAATGCGAAAACATTAAGAAATGACAACTCAAGTCGCTTTGGAAAATTGATTGAAATCCACTTTAGTGAAACTGGAAAGATATCTGGTGCCAACATTCAAACTTTTTTGCTTGAAAAGTCTAGAGTCGTCCAATGCACTGAGGGAGAAAGGTCATACCATATATTTTATCAGCTGTGTGCTGGAGCTCCACCTGCTCTTAGAGAGATGCTGAACTTGAAGAGTGCAGATGAGTACAAATATCTGCAGCAAAGCAATTGTTATTCCATTACTGGAGTTGATGATGCCGAAGAATTTCGTGTGGTCAAGGAAGCTCTAGATGTTGTCCATATTAACAAGGAAGACCAAGAAAGTGTCTTTGCAATGCTTGCTGCAGTCTTGTGGCTGGGAAACATCTCATTTAGTGTGATTGATAATGAAAATCATGTGGAAGCTGTGGCAGATGAAGGTCTGTTTACTGTTGCTAAATTGGTTGGATGTAGCCCGGAGGAGCTTACGCTTGCTTTATCAACTCGCAAAATGCGAGTTGGTAATGACAATATTGTCCAAAAGCTAACACTATCACAGGCTGTCGATACGAGAGATGCTTTGGCAAAGTCGATCTATGCATGTTTGTTTGAGTGGTTGGTTGAACAAATTAACAAATCACTTGCAGTAGGAAAGCGGCGTACTGGCAGATCTATTAGCATTCTGGATATCTATGGTTTTGAATCCTTTGATAGAAATAGTTTTGAACAATTCTGCATTAATTATGCAAATGAGAGATTGCAGCAGCACTTCAATCGCCACTTGTTCAAACTAGAGCAGGAGGAATATATCCAGGATGGCATTGATTGGACAAGAGTTGAATTTGAAGACAACCAAGATTGCCTTGGCCTTTTTGAAAAGAGACCGTTGGGATTACTGTCCCTCTTAGATGAAGAGTCCACTTTCCCAAATGGTAGTGATTTGACCTTCGCCAACAAGCTTAAGCAACATCTGAGTTCTAATTCTTCTTTTAGAGGAGAACGAGACAAAGCCTTTACTGTCAGTCATTATGCTGGAGAGGTTACATATGACACAACAGGTTTTTTGGAGAAGAATAGAGACTTGCTGCATTTGGATTCCATTCAGCTTCTTTCCTCATGCTCATGCCACCTTCCTCAGATATTTGCATCTAGTATGCTCAATCGATCTGAGAAGCCTGTAGTTGGCCCCTTGCATAAATTAGGTGGAGCAGACTCCCAGAAGCTGAGTGTTGCAACAAAATTTAAGAGCCAATTGTTCCTACTGATGAAACGTCTTGAGAACACCACTCCACATTTCATACGTTGTATCAAGCCCAACAATAGTCAATCTCCAGGAATATATGAGCAAGGACTTGTCCTGCAGCAACTAAGATGTTGTGGAGTCCTTGAAGTGGTTCGAATATCGAGATCTGGCTTTCCTACCAGAATGTCTCACCAGAAGTTTGCAAGAAGATATGGTTTTCTTCTGCTGGAGAATGTTGCATCACAGGAGCCACTTAGTGTTTCAGTTGCTATTCTTCATCAGTTCAATATATTACCAGAGATGTATCAAGTGGGCTATACAAAATTGTTTTTCCGTACTGGACAGATTGGGGTGCTTGAGGATACCAGAAATCGTACACTCCATGGTATTTTACGCGTTCAAAGCTGCTTTAGAGGACACCAAGCTCGATGTTACCTTAAGGAGCTGAGAAGAGGAATCACCACGCTTCAATCCTTTGTAAGGGGAGAGAAAACGAGAAAGGAGTATGCAGTTTTACTACAGAGGCATAGAGCTGCTGTATTCATACAAAAGCTGATGAAAAGCAGGATTGCAAGGAAGAAATTCATTAACATTTGTGATGCATCAATCGTGATACAATCAGTTTATCGTGGCTGGTTTGTCAGAAGATGCTCAGGAGGTATAGGATTAATGAAACCTGGGAGCACAAAGGCGAACGAGTCAGATGAGGTGCTGGTCAAATCATCTTTTCTAGCTGAATTACAACGTCGTGTTCTTAAGGCTGAAGCTGCCTTGAGAGAGAAGGAAGAGGAGAACGACATTCTCCACCAACGCCTCCAACAGTATGAGAACCGCTGGTCTGAATATGAGCTGAAAATGAAATCCATGGAAGAAGTATGGCAGAAACAAATGAGATCCCTGCAATCCAGCCTCTCAATTGCCAAGAAGAGCCTTGCCATTGATGATTCTGAGAGAAATTCTGATGCATCGGTCAATGCAAGTGATGATCGCGACTACAGTTGGGATACAGGAAGCAACCATAAAGGCCAAGATAGCAATGGGGGAAGACCCATGAGTGCCGGTTTGAGTGTTATAAGCCGGTTGACTGAAGAATTTGACCAGAGGAGTCAAGTATTTGGTGATGACGCCAAGTTCTTGGTGGAGGTAAAATCTGGTCAAGTTGAAGCAAGTCTGAATCCAGACCGAGAGCTTAGAAGGTTGAAGCAGATGTTTGAGGCTTGGAAGAAGGATTATGGTGCAAGACTTCGGGAGACAAAGGTGGTGTTGACTAAGCTTGGAAATGAAGAGGGTACTGTTGATAGGGCAAAAAAGAAGTGGTGGGGAAGGAGGAGCAGCTCGAGGATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_head

Protein Analysis

1179

Amino Acids

133.37

Weight (kDa)

8.5

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_VIII-1_N PF25369 132 - 176 2e-24 Myosin-1-like, N-terminal SH3 domain
Myosin_head PF00063 184 - 842 7.8e-244 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 497, 812, 3263
AarI CACCTGC 1 cut(s) 1141
AasI GACNNNNNNGTC 1 cut(s) 1481
Acc36I ACCTGC 1 cut(s) 1141
Acc65I GGTACC 1 cut(s) 405
AccB1I GGYRCC 2 cut(s) 405, 1028
AccII CGCG 2 cut(s) 2586, 3180
AciI CCGC 4 cut(s) 55, 644, 1603, 3022
AclWI GGATC 1 cut(s) 3078
AcsI RAATTY 6 cut(s) 1232, 1787, 2186, 2771, 3145, 3281
AcuI CTGAAG 7 cut(s) 213, 291, 438, 531, 590, 2973, 3297
AcyI GRCGYC 1 cut(s) 3315
AdeI CACNNNGTG 1 cut(s) 2447
AfaI GTAC 7 cut(s) 407, 599, 1175, 1609, 2527, 2566, 3481
AfiI CCNNNNNNNGG 5 cut(s) 251, 778, 2129, 2338, 2670
AflII CTTAAG 3 cut(s) 1907, 2627, 2945
AflIII ACRYGT 2 cut(s) 742, 2584
AhdI GACNNNNNGTC 2 cut(s) 280, 370
AjiI CACGTC 1 cut(s) 372
AjnI CCWGG 5 cut(s) 150, 300, 1758, 2278, 2866
AleI CACNNNNGTG 1 cut(s) 1005
Alw21I GWGCWC 2 cut(s) 1129, 2876
Alw26I GTCTC 6 cut(s) 268, 1822, 1944, 2019, 2382, 3434
AlwI GGATC 1 cut(s) 3078
AlwNI CAGNNNCTG 2 cut(s) 461, 2170
Ama87I CYCGRG 2 cut(s) 203, 3526
AoxI GGCC 4 cut(s) 206, 1813, 2131, 3214
ApoI RAATTY 6 cut(s) 1232, 1787, 2186, 2771, 3145, 3281
AseI ATTAAT 2 cut(s) 1686, 2858
Asp700I GAANNNNTTC 4 cut(s) 780, 1233, 2340, 2771
Asp718I GGTACC 1 cut(s) 405
AspS9I GGNCC 6 cut(s) 116, 206, 223, 298, 647, 2132
AsuC2I CCSGG 3 cut(s) 204, 205, 1424
AsuHPI GGTGA 7 cut(s) 182, 227, 234, 374, 2372, 2641, 3320
AsuII TTCGAA 1 cut(s) 2344
AvaI CYCGRG 2 cut(s) 203, 3526
AvaII GGWCC 4 cut(s) 116, 223, 298, 647
BanI GGYRCC 2 cut(s) 405, 1028
BanII GRGCYC 1 cut(s) 1129
BbsI GAAGAC 4 cut(s) 279, 1287, 1800, 3241
Bbv12I GWGCWC 2 cut(s) 1129, 2876
BccI CCATC 5 cut(s) 539, 595, 761, 776, 1757
BcgI CGANNNNNNTGC 4 cut(s) 29, 63, 1703, 1737
BciT130I CCWGG 5 cut(s) 152, 302, 1760, 2280, 2868
BciVI GTATCC 2 cut(s) 2545, 3187
BcnI CCSGG 3 cut(s) 204, 205, 1424
BcoDI GTCTC 6 cut(s) 268, 1822, 1944, 2019, 2382, 3434
BfaI CTAG 7 cut(s) 113, 929, 1062, 1256, 1739, 2097, 2921
BfmI CTRYAG 6 cut(s) 1184, 1311, 2124, 2306, 2708, 3184
BfrI CTTAAG 3 cut(s) 1907, 2627, 2945
BfuAI ACCTGC 1 cut(s) 1141
BfuI GTATCC 2 cut(s) 2545, 3187
BglII AGATCT 2 cut(s) 1616, 2354
Bme1390I CCNGG 8 cut(s) 152, 204, 205, 302, 1424, 1760, 2280, 2868
Bme18I GGWCC 4 cut(s) 116, 223, 298, 647
BmeRI GACNNNNNGTC 2 cut(s) 280, 370
BmeT110I CYCGRG 2 cut(s) 203, 3526
BmgBI CACGTC 1 cut(s) 372
BmgT120I GGNCC 6 cut(s) 116, 206, 223, 298, 647, 2132
BmiI GGNNCC 6 cut(s) 407, 522, 648, 1030, 2134, 2439
BmrFI CCNGG 8 cut(s) 152, 204, 205, 302, 1424, 1760, 2280, 2868
BmrI ACTGGG 1 cut(s) 443
BmuI ACTGGG 1 cut(s) 443
BpiI GAAGAC 4 cut(s) 279, 1287, 1800, 3241
BpmI CTGGAG 5 cut(s) 1143, 1233, 2001, 2262, 2438
Bpu10I CCTNAGC 1 cut(s) 2842
Bpu14I TTCGAA 1 cut(s) 2344
BpuEI CTTGAG 4 cut(s) 955, 2243, 2567, 2983
BpuMI CCSGG 3 cut(s) 204, 205, 1424
Bsa29I ATCGAT 2 cut(s) 803, 2110
BsaBI GATNNNNATC 1 cut(s) 995
BsaHI GRCGYC 1 cut(s) 3315
BsaI GGTCTC 1 cut(s) 1822
BsaJI CCNNGG 5 cut(s) 203, 1810, 2572, 2867, 3054
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 5 cut(s) 251, 778, 2129, 2338, 2670
Bse118I RCCGGY 3 cut(s) 251, 3248, 3267
Bse1I ACTGG 7 cut(s) 132, 438, 650, 1018, 1216, 1615, 2533
Bse3DI GCAATG 5 cut(s) 145, 186, 864, 1308, 3232
Bse8I GATNNNNATC 1 cut(s) 995
BseBI CCWGG 5 cut(s) 152, 302, 1760, 2280, 2868
BseCI ATCGAT 2 cut(s) 803, 2110
BseDI CCNNGG 5 cut(s) 203, 1810, 2572, 2867, 3054
BseGI GGATG 4 cut(s) 559, 768, 1421, 1768
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 5 cut(s) 251, 778, 2129, 2338, 2670
BseMI GCAATG 5 cut(s) 145, 186, 864, 1308, 3232
BseMII CTCAG 9 cut(s) 63, 1071, 1910, 2096, 2106, 2162, 2627, 2856, 3129
BseNI ACTGG 7 cut(s) 132, 438, 650, 1018, 1216, 1615, 2533
BseRI GAGGAG 5 cut(s) 1442, 1956, 2993, 3307, 3532
BseYI CCCAGC 2 cut(s) 380, 1324
BsgI GTGCAG 2 cut(s) 858, 1185
Bsh1236I CGCG 2 cut(s) 2586, 3180
BshFI GGCC 4 cut(s) 208, 1815, 2133, 3216
BshNI GGYRCC 2 cut(s) 405, 1028
BshVI ATCGAT 2 cut(s) 803, 2110
BsiHKAI GWGCWC 2 cut(s) 1129, 2876
BsiHKCI CYCGRG 2 cut(s) 203, 3526
BsiSI CCGG 5 cut(s) 204, 252, 1424, 3249, 3268
BslFI GGGAC 3 cut(s) 186, 660, 1831
BslI CCNNNNNNNGG 5 cut(s) 251, 778, 2129, 2338, 2670
BsmAI GTCTC 6 cut(s) 268, 1822, 1944, 2019, 2382, 3434
BsmFI GGGAC 3 cut(s) 186, 660, 1831
BsmI GAATGC 1 cut(s) 1626
BsnI GGCC 4 cut(s) 208, 1815, 2133, 3216
Bso31I GGTCTC 1 cut(s) 1822
BsoBI CYCGRG 2 cut(s) 203, 3526
Bsp119I TTCGAA 1 cut(s) 2344
Bsp1286I GDGCHC 2 cut(s) 1129, 2876
Bsp1407I TGTACA 1 cut(s) 597
Bsp143I GATC 7 cut(s) 556, 1539, 1616, 2111, 2354, 3083, 3175
Bsp19I CCATGG 2 cut(s) 2572, 3054
Bsp68I TCGCGA 1 cut(s) 3180
BspACI CCGC 4 cut(s) 55, 644, 1603, 3022
BspANI GGCC 4 cut(s) 208, 1815, 2133, 3216
BspCNI CTCAG 9 cut(s) 64, 1072, 1911, 2095, 2107, 2163, 2628, 2855, 3130
BspDI ATCGAT 2 cut(s) 803, 2110
BspFNI CGCG 2 cut(s) 2586, 3180
BspLI GGNNCC 6 cut(s) 407, 522, 648, 1030, 2134, 2439
BspMAI CTGCAG 3 cut(s) 1188, 1315, 2310
BspMI ACCTGC 1 cut(s) 1141
BspPI GGATC 1 cut(s) 3078
BspQI GCTCTTC 1 cut(s) 3110
BspT104I TTCGAA 1 cut(s) 2344
BspT107I GGYRCC 2 cut(s) 405, 1028
BspTI CTTAAG 3 cut(s) 1907, 2627, 2945
BspTNI GGTCTC 1 cut(s) 1822
BsrDI GCAATG 5 cut(s) 145, 186, 864, 1308, 3232
BsrFI RCCGGY 3 cut(s) 251, 3248, 3267
BsrGI TGTACA 1 cut(s) 597
BsrI ACTGG 7 cut(s) 132, 438, 650, 1018, 1216, 1615, 2533
BssAI RCCGGY 3 cut(s) 251, 3248, 3267
BssECI CCNNGG 5 cut(s) 203, 1810, 2572, 2867, 3054
BssMI GATC 7 cut(s) 556, 1539, 1616, 2111, 2354, 3083, 3175
BssNI GRCGYC 1 cut(s) 3315
BssT1I CCWWGG 3 cut(s) 1810, 2572, 3054
Bst2UI CCWGG 5 cut(s) 152, 302, 1760, 2280, 2868
Bst4CI ACNGT 9 cut(s) 262, 469, 1399, 1833, 1845, 1966, 3012, 3188, 3484
Bst6I CTCTTC 6 cut(s) 1154, 1854, 2635, 2970, 3110, 3468
BstACI GRCGYC 1 cut(s) 3315
BstAFI CTTAAG 3 cut(s) 1907, 2627, 2945
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstAUI TGTACA 1 cut(s) 597
BstBI TTCGAA 1 cut(s) 2344
BstC8I GCNNGC 3 cut(s) 38, 1308, 1440
BstDSI CCRYGG 2 cut(s) 2572, 3054
BstENI CCTNNNNNAGG 1 cut(s) 2668
BstF5I GGATG 4 cut(s) 559, 768, 1421, 1768
BstFNI CGCG 2 cut(s) 2586, 3180
BstKTI GATC 7 cut(s) 559, 1542, 1619, 2114, 2357, 3086, 3178
BstMAI GTCTC 6 cut(s) 268, 1822, 1944, 2019, 2382, 3434
BstMBI GATC 7 cut(s) 556, 1539, 1616, 2111, 2354, 3083, 3175
BstMWI GCNNNNNNNGC 6 cut(s) 854, 1133, 1307, 2592, 2957, 3222
BstNI CCWGG 5 cut(s) 152, 302, 1760, 2280, 2868
BstNSI RCATGY 2 cut(s) 746, 1551
BstSCI CCNGG 8 cut(s) 150, 202, 203, 300, 1422, 1758, 2278, 2866
BstSFI CTRYAG 6 cut(s) 1184, 1311, 2124, 2306, 2708, 3184
BstUI CGCG 2 cut(s) 2586, 3180
BstV2I GAAGAC 4 cut(s) 279, 1287, 1800, 3241
BstX2I RGATCY 3 cut(s) 1616, 2354, 3083
BstXI CCANNNNNNTGG 1 cut(s) 3325
BstYI RGATCY 3 cut(s) 1616, 2354, 3083
Bsu15I ATCGAT 2 cut(s) 803, 2110
BsuI GTATCC 2 cut(s) 2545, 3187
BsuRI GGCC 4 cut(s) 208, 1815, 2133, 3216
BsuTUI ATCGAT 2 cut(s) 803, 2110
BtgI CCRYGG 2 cut(s) 2572, 3054
BtrI CACGTC 1 cut(s) 372
BtsCI GGATG 4 cut(s) 559, 768, 1421, 1768
BtsI GCAGTG 1 cut(s) 476
BtsIMutI CAGTG 7 cut(s) 258, 267, 459, 476, 597, 657, 1077
BtuMI TCGCGA 1 cut(s) 3180
BveI ACCTGC 1 cut(s) 1141
Cac8I GCNNGC 3 cut(s) 38, 1308, 1440
CaiI CAGNNNCTG 2 cut(s) 461, 2170
Cfr10I RCCGGY 3 cut(s) 251, 3248, 3267
Cfr13I GGNCC 6 cut(s) 116, 206, 223, 298, 647, 2132
Cfr9I CCCGGG 1 cut(s) 203
ClaI ATCGAT 2 cut(s) 803, 2110
CseI GACGC 1 cut(s) 3323
Csp6I GTAC 7 cut(s) 406, 598, 1174, 1608, 2526, 2565, 3480
CviQI GTAC 7 cut(s) 406, 598, 1174, 1608, 2526, 2565, 3480
DpnI GATC 7 cut(s) 558, 1541, 1618, 2113, 2356, 3085, 3177
DpnII GATC 7 cut(s) 556, 1539, 1616, 2111, 2354, 3083, 3175
DraIII CACNNNGTG 1 cut(s) 2447
DrdI GACNNNNNNGTC 1 cut(s) 1481
DriI GACNNNNNGTC 2 cut(s) 280, 370
DseDI GACNNNNNNGTC 1 cut(s) 1481
Eam1104I CTCTTC 6 cut(s) 1154, 1854, 2635, 2970, 3110, 3468
Eam1105I GACNNNNNGTC 2 cut(s) 280, 370
EarI CTCTTC 6 cut(s) 1154, 1854, 2635, 2970, 3110, 3468
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 1127
Eco130I CCWWGG 3 cut(s) 1810, 2572, 3054
Eco24I GRGCYC 1 cut(s) 1129
Eco31I GGTCTC 1 cut(s) 1822
Eco32I GATATC 3 cut(s) 538, 1023, 1636
Eco47I GGWCC 4 cut(s) 116, 223, 298, 647
Eco53kI GAGCTC 1 cut(s) 1127
Eco57I CTGAAG 7 cut(s) 213, 291, 438, 531, 590, 2973, 3297
Eco88I CYCGRG 2 cut(s) 203, 3526
EcoICRI GAGCTC 1 cut(s) 1127
EcoNI CCTNNNNNAGG 1 cut(s) 2668
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 5 cut(s) 150, 300, 1758, 2278, 2866
EcoRV GATATC 3 cut(s) 538, 1023, 1636
EcoT14I CCWWGG 3 cut(s) 1810, 2572, 3054
EcoT22I ATGCAT 3 cut(s) 1549, 2794, 3157
EcoT38I GRGCYC 1 cut(s) 1129
ErhI CCWWGG 3 cut(s) 1810, 2572, 3054
FalI AAGNNNNNCTT 2 cut(s) 1794, 1826
FaqI GGGAC 3 cut(s) 186, 660, 1831
FauI CCCGC 1 cut(s) 637
FauNDI CATATG 2 cut(s) 376, 1993
FokI GGATG 4 cut(s) 566, 755, 1428, 1775
FriOI GRGCYC 1 cut(s) 1129
FspBI CTAG 7 cut(s) 113, 929, 1062, 1256, 1739, 2097, 2921
GsaI CCCAGC 2 cut(s) 384, 1328
GsuI CTGGAG 5 cut(s) 1143, 1233, 2001, 2262, 2438
HaeIII GGCC 4 cut(s) 208, 1815, 2133, 3216
HapII CCGG 5 cut(s) 204, 252, 1424, 3249, 3268
HgaI GACGC 1 cut(s) 3323
Hin1I GRCGYC 1 cut(s) 3315
HincII GTYRAC 3 cut(s) 450, 3273, 3456
HindII GTYRAC 3 cut(s) 450, 3273, 3456
HindIII AAGCTT 2 cut(s) 1904, 3461
HpaII CCGG 5 cut(s) 204, 252, 1424, 3249, 3268
HphI GGTGA 7 cut(s) 182, 227, 234, 374, 2372, 2641, 3320
Hpy166II GTNNAC 9 cut(s) 119, 173, 450, 740, 1395, 1866, 2567, 3273, 3456
Hpy8I GTNNAC 9 cut(s) 119, 173, 450, 740, 1395, 1866, 2567, 3273, 3456
Hpy99I CGWCG 2 cut(s) 357, 2940
HpyCH4III ACNGT 9 cut(s) 262, 469, 1399, 1833, 1845, 1966, 3012, 3188, 3484
HpyCH4IV ACGT 5 cut(s) 352, 371, 2218, 2248, 2935
HpyF10VI GCNNNNNNNGC 6 cut(s) 854, 1133, 1307, 2592, 2957, 3222
HpySE526I ACGT 5 cut(s) 352, 371, 2218, 2248, 2935
Hsp92I GRCGYC 1 cut(s) 3315
KpnI GGTACC 1 cut(s) 409
Kzo9I GATC 7 cut(s) 556, 1539, 1616, 2111, 2354, 3083, 3175
LguI GCTCTTC 1 cut(s) 3110
LmnI GCTCC 9 cut(s) 827, 1124, 1132, 1429, 2153, 2437, 2632, 2871, 3519
MaeI CTAG 7 cut(s) 113, 929, 1062, 1256, 1739, 2097, 2921
MaeII ACGT 5 cut(s) 352, 371, 2218, 2248, 2935
MaeIII GTNAC 8 cut(s) 101, 215, 310, 362, 457, 572, 1987, 2621
MalI GATC 7 cut(s) 558, 1541, 1618, 2113, 2356, 3085, 3177
MboI GATC 7 cut(s) 556, 1539, 1616, 2111, 2354, 3083, 3175
MfeI CAATTG 3 cut(s) 1195, 2198, 3105
MflI RGATCY 3 cut(s) 1616, 2354, 3083
MhlI GDGCHC 2 cut(s) 1129, 2876
MluI ACGCGT 1 cut(s) 2584
MlyI GAGTC 6 cut(s) 1074, 1871, 2153, 2337, 2897, 3304
MmeI TCCRAC 2 cut(s) 1393, 3031
Mph1103I ATGCAT 3 cut(s) 1549, 2794, 3157
MroXI GAANNNNTTC 4 cut(s) 780, 1233, 2340, 2771
MslI CAYNNNNRTG 3 cut(s) 747, 1005, 2068
MspA1I CMGCKG 2 cut(s) 1114, 3024
MspCI CTTAAG 3 cut(s) 1907, 2627, 2945
MspI CCGG 5 cut(s) 204, 252, 1424, 3249, 3268
MspR9I CCNGG 8 cut(s) 152, 204, 205, 302, 1424, 1760, 2280, 2868
MunI CAATTG 3 cut(s) 1195, 2198, 3105
Mva1269I GAATGC 1 cut(s) 1626
MvaI CCWGG 5 cut(s) 152, 302, 1760, 2280, 2868
MvnI CGCG 2 cut(s) 2586, 3180
MwoI GCNNNNNNNGC 6 cut(s) 854, 1133, 1307, 2592, 2957, 3222
NciI CCSGG 3 cut(s) 204, 205, 1424
NcoI CCATGG 2 cut(s) 2572, 3054
NdeI CATATG 2 cut(s) 376, 1993
NdeII GATC 7 cut(s) 556, 1539, 1616, 2111, 2354, 3083, 3175
NlaIV GGNNCC 6 cut(s) 407, 522, 648, 1030, 2134, 2439
NmuCI GTSAC 4 cut(s) 101, 215, 362, 457
NruI TCGCGA 1 cut(s) 3180
NsiI ATGCAT 3 cut(s) 1549, 2794, 3157
NspI RCATGY 2 cut(s) 746, 1551
NspV TTCGAA 1 cut(s) 2344
OliI CACNNNNGTG 1 cut(s) 1005
PaeR7I CTCGAG 1 cut(s) 3526
PaqCI CACCTGC 1 cut(s) 1141
PciI ACATGT 1 cut(s) 742
PciSI GCTCTTC 1 cut(s) 3110
PcsI WCGNNNNNNNCGW 2 cut(s) 349, 1459
PctI GAATGC 1 cut(s) 1626
PdmI GAANNNNTTC 4 cut(s) 780, 1233, 2340, 2771
PfeI GAWTC 8 cut(s) 58, 239, 305, 1649, 2042, 2646, 3134, 3364
PfoI TCCNGGA 2 cut(s) 1758, 2278
PleI GAGTC 6 cut(s) 1073, 1870, 2153, 2336, 2896, 3303
PpsI GAGTC 6 cut(s) 1073, 1870, 2153, 2336, 2896, 3303
PpuMI RGGWCCY 1 cut(s) 298
PscI ACATGT 1 cut(s) 742
PshBI ATTAAT 2 cut(s) 1686, 2858
PsiI TTATAA 3 cut(s) 497, 812, 3263
Psp124BI GAGCTC 1 cut(s) 1129
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 5 cut(s) 150, 300, 1758, 2278, 2866
PspFI CCCAGC 2 cut(s) 380, 1324
PspGI CCWGG 5 cut(s) 150, 300, 1758, 2278, 2866
PspN4I GGNNCC 6 cut(s) 407, 522, 648, 1030, 2134, 2439
PspPI GGNCC 6 cut(s) 116, 206, 223, 298, 647, 2132
PspPPI RGGWCCY 1 cut(s) 298
PspXI VCTCGAGB 1 cut(s) 3526
PstI CTGCAG 3 cut(s) 1188, 1315, 2310
PstNI CAGNNNCTG 2 cut(s) 461, 2170
PsuI RGATCY 3 cut(s) 1616, 2354, 3083
PvuII CAGCTG 1 cut(s) 1114
RruI TCGCGA 1 cut(s) 3180
RsaI GTAC 7 cut(s) 407, 599, 1175, 1609, 2527, 2566, 3481
RsaNI GTAC 7 cut(s) 406, 598, 1174, 1608, 2526, 2565, 3480
RseI CAYNNNNRTG 3 cut(s) 747, 1005, 2068
SacI GAGCTC 1 cut(s) 1129
SapI GCTCTTC 1 cut(s) 3110
Sau3AI GATC 7 cut(s) 556, 1539, 1616, 2111, 2354, 3083, 3175
Sau96I GGNCC 6 cut(s) 116, 206, 223, 298, 647, 2132
SchI GAGTC 6 cut(s) 1074, 1871, 2153, 2337, 2897, 3304
ScrFI CCNGG 8 cut(s) 152, 204, 205, 302, 1424, 1760, 2280, 2868
SduI GDGCHC 2 cut(s) 1129, 2876
SfcI CTRYAG 6 cut(s) 1184, 1311, 2124, 2306, 2708, 3184
Sfr274I CTCGAG 1 cut(s) 3526
SfuI TTCGAA 1 cut(s) 2344
SinI GGWCC 4 cut(s) 116, 223, 298, 647
SlaI CTCGAG 1 cut(s) 3526
SmaI CCCGGG 1 cut(s) 205
SmiMI CAYNNNNRTG 3 cut(s) 747, 1005, 2068
SmlI CTYRAG 8 cut(s) 970, 1907, 2222, 2546, 2627, 2945, 2962, 3526
SmoI CTYRAG 8 cut(s) 970, 1907, 2222, 2546, 2627, 2945, 2962, 3526
SsiI CCGC 4 cut(s) 55, 644, 1603, 3022
SspI AATATT 4 cut(s) 444, 866, 909, 1480
SspMI CTAG 7 cut(s) 113, 929, 1062, 1256, 1739, 2097, 2921
SstI GAGCTC 1 cut(s) 1129
StyD4I CCNGG 8 cut(s) 150, 202, 203, 300, 1422, 1758, 2278, 2866
StyI CCWWGG 3 cut(s) 1810, 2572, 3054
TaaI ACNGT 9 cut(s) 262, 469, 1399, 1833, 1845, 1966, 3012, 3188, 3484
TaiI ACGT 5 cut(s) 355, 374, 2221, 2251, 2938
TaqII GACCGA 2 cut(s) 3148, 3387
TatI WGTACW 2 cut(s) 597, 1173
TauI GCSGC 1 cut(s) 1606
TfiI GAWTC 8 cut(s) 58, 239, 305, 1649, 2042, 2646, 3134, 3364
TscAI CASTG 7 cut(s) 265, 274, 466, 483, 597, 657, 1084
TseFI GTSAC 4 cut(s) 101, 215, 362, 457
Tsp45I GTSAC 4 cut(s) 101, 215, 362, 457
TspGWI ACGGA 1 cut(s) 2513
TspMI CCCGGG 1 cut(s) 203
TspRI CASTG 7 cut(s) 265, 274, 466, 483, 597, 657, 1084
Vha464I CTTAAG 3 cut(s) 1907, 2627, 2945
VpaK11BI GGWCC 4 cut(s) 116, 223, 298, 647
VspI ATTAAT 2 cut(s) 1686, 2858
XagI CCTNNNNNAGG 1 cut(s) 2668
XapI RAATTY 6 cut(s) 1232, 1787, 2186, 2771, 3145, 3281
XbaI TCTAGA 2 cut(s) 1061, 1255
XceI RCATGY 2 cut(s) 746, 1551
XcmI CCANNNNNNNNNTGG 6 cut(s) 416, 423, 775, 1808, 3224, 3325
XhoI CTCGAG 1 cut(s) 3526
XmaI CCCGGG 1 cut(s) 203
XmnI GAANNNNTTC 4 cut(s) 780, 1233, 2340, 2771
XspI CTAG 7 cut(s) 113, 929, 1062, 1256, 1739, 2097, 2921
Zsp2I ATGCAT 3 cut(s) 1549, 2794, 3157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.