MD13G1013000.v1.1

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005217
Physical Location & Seq
Forward (+)
794861 .. 799415
4555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1013000.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGCTACCGCCGCAGCCACAGGCACAGCCCCACCACCTCAAAAGAAAAGGAGAAATCAACCAGGAAATCCAAGCAAATCCAGATGCGGAGGTTATAGCACTATCTCCCAAGACGCTAATGGCGACAAACAGGTTCATATGTGAGGTGTGCAACAAAGGGTTCCAAAGGGAGCAAAACCTACAGCTCCACAGAAGAGGACACAACCTGCCTTGGAAGCTCAAGCAGAAGACTAACAAAGAACCTAGACGCAAGGTCTATCTATGTCCGGAGCCGTCATGCGTTCACCATGACCCCTCTCGAGCTCTCGGAGACCTCACCGGCATAAAAAAACACTACTCTAGAAAGCACGGCGAGAAGAAGTGGAAGTGCGACAAGTGCTCCAAGAGGTATGCCGTTCAATCGGATTGGAAGGCTCATTCTAAAACGTGCGGAACTAGGGAATACAGATGCGACTGTGGCACTCTATTCTCAAGGCGGGACAGTTTTATCACTCATAGGGCTTTCTGTGATGCACTGGCTCAGGAAAGTGCAAGGCATCCTTCAAGCTTAAGCACAATGGGCGGTAGCCTCTATGGAAACAACCATATGAGCTTAGGTCCATCCCAAATCCCCTCACTCCAAAACTCAACCCACCTCCCTAACAACGTGTTGAGGTTAGGGAGTAGCGGCAGCGCAAAGTTTGAGCACCTCATCCCGCCATCAAATCCGTCTTCGTTTGGATCACAAGCCATGCCTAATTCTCCCAATTTCTTCATGAGAGACATTGCTAACCAGCAAGGGTTTCAAGATCAGCACCAATTTCCAAACAAGGTATTACATGGACTAATGCAACTTCCTGATCTCCAGAGCAACCCTAACAACAACAGCAGCTCACCCTCATCGAATAGCGCTGCCAATCTCTTTAACCTGAGCTTCTTTTCTAACAATGGTACCAGTCGCAGCATAACTCCTACTGATCATCAGTTTAGTGATGGTAATGGCGGTGGCCAAGGGTTTAGCATGAGTCATGACCATCAAATTGGGTCGGGTTCTCTACCATCTCTCTATGGGAATTCGATGCAACACGAAGGCGGCATGGCTCCGCACATGTCTGCCACTGCATTGCTTCAGAAAGCTGCTCAAATGGGTTCGACTACAAGCACCGAAAGCTCGTCTTTGCTTAGGGGATTAGGTAATTCGTCAACAAGAGCAGCTAAATCCGGAAGGCAACTAGTGTCAGCGCCCTCGAGCGTTGGAGTCAGTTTTAGTGGTGATCATCATCACAGTACTAATGGGGGTGAACAGTACCACTTGAGATCGTCACAGCAGGTGGAAAATGAAAACCATCTTCAAGGATTAATGAACTCTCTTGCAAATGGCAACTCCTCGATTTTTGGAGGCGGGGGACTGGATCATAACAACTTTGGCGGGTTTAGTAGCGGCACTACTGCTACTAGAGTTAGTGCAATGGATCAACAGCATAATAACCCTAATTATGATGAGGCTAAGTTGCACCAAAATTTGAGTGTAAATTTTGGAGGGTCTGATAAACTGACCTTGGATTTTCTTGGTGTTGGAGGCATGGTAAGAAACATGAATGGTGGCCATGGATACTCACAGAGAGATCATCAACAACAACAACATCATGGCATCAATATGATCAGCTCCTTAGACCCAGAGTTGAAGTCAACGCAGGCAAGTCAACTCTTTGGAGGCACCACACTACAGTGA

Protein Analysis

570

Amino Acids

62.04

Weight (kDa)

9.2

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 82 - 116 1.5e-22 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 120 - 144 1.2e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 147 - 185 3.3e-24 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1297
Acc36I ACCTGC 2 cut(s) 213, 1297
Acc65I GGTACC 1 cut(s) 929
AccB1I GGYRCC 2 cut(s) 929, 1694
AccIII TCCGGA 2 cut(s) 265, 1199
AclWI GGATC 3 cut(s) 727, 1398, 1458
AcoI YGGCCR 2 cut(s) 985, 1582
AcsI RAATTY 3 cut(s) 1051, 1498, 1510
AcuI CTGAAG 1 cut(s) 1091
AfaI GTAC 3 cut(s) 931, 1267, 1286
AfeI AGCGCT 1 cut(s) 889
AflII CTTAAG 1 cut(s) 547
AflIII ACRYGT 2 cut(s) 645, 1086
AgsI TTSAA 5 cut(s) 398, 543, 785, 1331, 1663
AhdI GACNNNNNGTC 1 cut(s) 251
AhlI ACTAGT 1 cut(s) 1210
AjnI CCWGG 1 cut(s) 60
AleI CACNNNNGTG 1 cut(s) 1705
Alw21I GWGCWC 3 cut(s) 304, 380, 687
Alw26I GTCTC 2 cut(s) 303, 753
AlwI GGATC 3 cut(s) 727, 1398, 1458
Ama87I CYCGRG 2 cut(s) 297, 1225
Aor13HI TCCGGA 2 cut(s) 265, 1199
Aor51HI AGCGCT 1 cut(s) 889
AoxI GGCC 2 cut(s) 985, 1582
ApeKI GCWGC 7 cut(s) 13, 669, 867, 890, 939, 1115, 1190
ApoI RAATTY 3 cut(s) 1051, 1498, 1510
AseI ATTAAT 1 cut(s) 1337
Asp718I GGTACC 1 cut(s) 929
AspLEI GCGC 3 cut(s) 674, 890, 1222
AspS9I GGNCC 1 cut(s) 596
AsuHPI GGTGA 5 cut(s) 275, 307, 864, 1262, 1289
AvaI CYCGRG 2 cut(s) 297, 1225
AvaII GGWCC 1 cut(s) 596
BaeI ACNNNNGTAYC 2 cut(s) 913, 946
BalI TGGCCA 2 cut(s) 987, 1584
BanI GGYRCC 2 cut(s) 929, 1694
BanII GRGCYC 1 cut(s) 304
BbsI GAAGAC 2 cut(s) 233, 702
Bbv12I GWGCWC 3 cut(s) 304, 380, 687
BbvI GCAGC 7 cut(s) 25, 681, 877, 879, 951, 1102, 1202
BccI CCATC 6 cut(s) 607, 706, 965, 1020, 1045, 1332
BceAI ACGGC 3 cut(s) 256, 364, 377
BciT130I CCWGG 1 cut(s) 62
BciVI GTATCC 1 cut(s) 1583
BclI TGATCA 3 cut(s) 955, 1252, 1638
BcoDI GTCTC 2 cut(s) 303, 753
BcuI ACTAGT 1 cut(s) 1210
BfaI CTAG 5 cut(s) 243, 339, 435, 1211, 1434
BfmI CTRYAG 2 cut(s) 179, 1703
BfoI RGCGCY 2 cut(s) 891, 1223
BfrI CTTAAG 1 cut(s) 547
BfuAI ACCTGC 2 cut(s) 213, 1297
BfuI GTATCC 1 cut(s) 1583
BmcAI AGTACT 1 cut(s) 1267
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 1 cut(s) 596
BmeRI GACNNNNNGTC 1 cut(s) 251
BmeT110I CYCGRG 2 cut(s) 297, 1225
BmgT120I GGNCC 1 cut(s) 596
BmiI GGNNCC 5 cut(s) 161, 270, 931, 1080, 1696
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 6 cut(s) 73, 437, 499, 544, 1047, 1638
BpiI GAAGAC 2 cut(s) 233, 702
BpmI CTGGAG 1 cut(s) 827
Bpu10I CCTNAGC 4 cut(s) 519, 592, 908, 1160
BpuEI CTTGAG 3 cut(s) 203, 454, 1312
BsaBI GATNNNNATC 1 cut(s) 1257
BsaI GGTCTC 1 cut(s) 303
BsaJI CCNNGG 4 cut(s) 209, 988, 1536, 1585
BsaWI WCCGGW 2 cut(s) 265, 1199
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 1548, 1578
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 3 cut(s) 519, 933, 1392
Bse3DI GCAATG 3 cut(s) 762, 1100, 1452
Bse8I GATNNNNATC 1 cut(s) 1257
BseAI TCCGGA 2 cut(s) 265, 1199
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 4 cut(s) 209, 988, 1536, 1585
BseGI GGATG 3 cut(s) 535, 599, 690
BseJI GATNNNNATC 1 cut(s) 1257
BseMI GCAATG 3 cut(s) 762, 1100, 1452
BseMII CTCAG 2 cut(s) 533, 899
BseNI ACTGG 3 cut(s) 519, 933, 1392
BseRI GAGGAG 1 cut(s) 1354
BseXI GCAGC 7 cut(s) 25, 681, 877, 879, 951, 1102, 1202
BshFI GGCC 2 cut(s) 987, 1584
BshNI GGYRCC 2 cut(s) 929, 1694
BsiHKAI GWGCWC 3 cut(s) 304, 380, 687
BsiHKCI CYCGRG 2 cut(s) 297, 1225
BsiSI CCGG 3 cut(s) 266, 318, 1200
BslFI GGGAC 2 cut(s) 491, 1398
BsmAI GTCTC 2 cut(s) 303, 753
BsmFI GGGAC 2 cut(s) 491, 1398
BsnI GGCC 2 cut(s) 987, 1584
Bso31I GGTCTC 1 cut(s) 303
BsoBI CYCGRG 2 cut(s) 297, 1225
Bsp1286I GDGCHC 3 cut(s) 304, 380, 687
Bsp13I TCCGGA 2 cut(s) 265, 1199
Bsp19I CCATGG 1 cut(s) 1585
BspANI GGCC 2 cut(s) 987, 1584
BspCNI CTCAG 2 cut(s) 532, 900
BspEI TCCGGA 2 cut(s) 265, 1199
BspHI TCATGA 2 cut(s) 753, 1006
BspLI GGNNCC 5 cut(s) 161, 270, 931, 1080, 1696
BspMI ACCTGC 2 cut(s) 213, 1297
BspPI GGATC 3 cut(s) 727, 1398, 1458
BspT107I GGYRCC 2 cut(s) 929, 1694
BspTI CTTAAG 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 303
BsrDI GCAATG 3 cut(s) 762, 1100, 1452
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 3 cut(s) 519, 933, 1392
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 4 cut(s) 209, 988, 1536, 1585
BssT1I CCWWGG 4 cut(s) 209, 988, 1536, 1585
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 5 cut(s) 455, 482, 1265, 1284, 1707
Bst6I CTCTTC 1 cut(s) 187
BstAFI CTTAAG 1 cut(s) 547
BstC8I GCNNGC 1 cut(s) 1674
BstDEI CTNAG 6 cut(s) 519, 592, 908, 1160, 1485, 1648
BstDSI CCRYGG 1 cut(s) 1585
BstF5I GGATG 3 cut(s) 535, 599, 690
BstH2I RGCGCY 2 cut(s) 891, 1223
BstHHI GCGC 3 cut(s) 674, 890, 1222
BstMAI GTCTC 2 cut(s) 303, 753
BstMWI GCNNNNNNNGC 6 cut(s) 10, 214, 375, 456, 558, 1146
BstNI CCWGG 1 cut(s) 62
BstNSI RCATGY 1 cut(s) 1090
BstSCI CCNGG 1 cut(s) 60
BstSFI CTRYAG 2 cut(s) 179, 1703
BstV1I GCAGC 7 cut(s) 25, 681, 877, 879, 951, 1102, 1202
BstV2I GAAGAC 2 cut(s) 233, 702
BstXI CCANNNNNNTGG 1 cut(s) 1019
BsuI GTATCC 1 cut(s) 1583
BsuRI GGCC 2 cut(s) 987, 1584
BtgI CCRYGG 1 cut(s) 1585
BtsCI GGATG 3 cut(s) 535, 599, 690
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 2 cut(s) 512, 1095
BveI ACCTGC 2 cut(s) 213, 1297
Cac8I GCNNGC 1 cut(s) 1674
CciI TCATGA 2 cut(s) 753, 1006
CfoI GCGC 3 cut(s) 674, 890, 1222
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 596
CseI GACGC 2 cut(s) 121, 255
Csp6I GTAC 3 cut(s) 930, 1266, 1285
CviQI GTAC 3 cut(s) 930, 1266, 1285
DdeI CTNAG 6 cut(s) 519, 592, 908, 1160, 1485, 1648
DriI GACNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 2 cut(s) 985, 1582
Eam1104I CTCTTC 1 cut(s) 187
Eam1105I GACNNNNNGTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 187
Ecl136II GAGCTC 1 cut(s) 302
Eco130I CCWWGG 4 cut(s) 209, 988, 1536, 1585
Eco24I GRGCYC 1 cut(s) 304
Eco31I GGTCTC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 596
Eco47III AGCGCT 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 1091
Eco88I CYCGRG 2 cut(s) 297, 1225
EcoICRI GAGCTC 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 1051
EcoRII CCWGG 1 cut(s) 60
EcoT14I CCWWGG 4 cut(s) 209, 988, 1536, 1585
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 4 cut(s) 209, 988, 1536, 1585
FalI AAGNNNNNCTT 4 cut(s) 523, 555, 1138, 1170
FaqI GGGAC 2 cut(s) 491, 1398
FauI CCCGC 4 cut(s) 468, 702, 1373, 1400
FauNDI CATATG 2 cut(s) 137, 585
FbaI TGATCA 3 cut(s) 955, 1252, 1638
FokI GGATG 3 cut(s) 522, 586, 677
FriOI GRGCYC 1 cut(s) 304
FspBI CTAG 5 cut(s) 243, 339, 435, 1211, 1434
GlaI GCGC 3 cut(s) 673, 889, 1221
GsuI CTGGAG 1 cut(s) 827
HaeII RGCGCY 2 cut(s) 891, 1223
HaeIII GGCC 2 cut(s) 987, 1584
HapII CCGG 3 cut(s) 266, 318, 1200
HgaI GACGC 2 cut(s) 121, 255
HhaI GCGC 3 cut(s) 674, 890, 1222
Hin6I GCGC 3 cut(s) 672, 888, 1220
HinP1I GCGC 3 cut(s) 672, 888, 1220
HincII GTYRAC 3 cut(s) 1182, 1668, 1682
HindII GTYRAC 3 cut(s) 1182, 1668, 1682
HindIII AAGCTT 1 cut(s) 544
HinfI GANTC 2 cut(s) 1003, 1236
HpaII CCGG 3 cut(s) 266, 318, 1200
HphI GGTGA 5 cut(s) 275, 307, 864, 1262, 1289
Hpy166II GTNNAC 5 cut(s) 283, 1182, 1280, 1668, 1682
Hpy188I TCNGA 4 cut(s) 308, 403, 1110, 1525
Hpy8I GTNNAC 5 cut(s) 283, 1182, 1280, 1668, 1682
HpyAV CCTTC 4 cut(s) 403, 549, 1061, 1197
HpyCH4III ACNGT 5 cut(s) 455, 482, 1265, 1284, 1707
HpyCH4IV ACGT 2 cut(s) 425, 645
HpyCH4V TGCA 9 cut(s) 150, 512, 530, 829, 1060, 1100, 1352, 1445, 1492
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 214, 375, 456, 558, 1146
HpyF3I CTNAG 6 cut(s) 519, 592, 908, 1160, 1485, 1648
HpySE526I ACGT 2 cut(s) 425, 645
HspAI GCGC 3 cut(s) 672, 888, 1220
Kpn2I TCCGGA 2 cut(s) 265, 1199
KpnI GGTACC 1 cut(s) 933
Ksp22I TGATCA 3 cut(s) 955, 1252, 1638
LmnI GCTCC 6 cut(s) 169, 189, 268, 383, 1084, 1649
Lsp1109I GCAGC 7 cut(s) 25, 681, 877, 879, 951, 1102, 1202
LweI GCATC 6 cut(s) 73, 437, 499, 544, 1047, 1638
MaeI CTAG 5 cut(s) 243, 339, 435, 1211, 1434
MaeII ACGT 2 cut(s) 425, 645
MaeIII GTNAC 1 cut(s) 1299
MboII GAAGA 6 cut(s) 204, 238, 367, 702, 742, 1319
MhlI GDGCHC 3 cut(s) 304, 380, 687
MlsI TGGCCA 2 cut(s) 987, 1584
MluCI AATT 9 cut(s) 736, 745, 797, 1017, 1051, 1174, 1471, 1498, 1510
MluNI TGGCCA 2 cut(s) 987, 1584
MlyI GAGTC 2 cut(s) 1012, 1245
MmeI TCCRAC 2 cut(s) 1213, 1534
Mox20I TGGCCA 2 cut(s) 987, 1584
MroI TCCGGA 2 cut(s) 265, 1199
MscI TGGCCA 2 cut(s) 987, 1584
MseI TTAA 3 cut(s) 548, 903, 1337
MslI CAYNNNNRTG 2 cut(s) 1634, 1705
Msp20I TGGCCA 2 cut(s) 987, 1584
MspCI CTTAAG 1 cut(s) 547
MspI CCGG 3 cut(s) 266, 318, 1200
MspR9I CCNGG 1 cut(s) 62
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 6 cut(s) 10, 214, 375, 456, 558, 1146
NcoI CCATGG 1 cut(s) 1585
NdeI CATATG 2 cut(s) 137, 585
NlaIV GGNNCC 5 cut(s) 161, 270, 931, 1080, 1696
NmuCI GTSAC 1 cut(s) 1299
NspI RCATGY 1 cut(s) 1090
OliI CACNNNNGTG 1 cut(s) 1705
PaeR7I CTCGAG 2 cut(s) 297, 1225
PagI TCATGA 2 cut(s) 753, 1006
PaqCI CACCTGC 1 cut(s) 1297
PciI ACATGT 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PleI GAGTC 2 cut(s) 1011, 1244
PpsI GAGTC 2 cut(s) 1011, 1244
PscI ACATGT 1 cut(s) 1086
PshBI ATTAAT 1 cut(s) 1337
Psp124BI GAGCTC 1 cut(s) 304
Psp6I CCWGG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 5 cut(s) 161, 270, 931, 1080, 1696
PspPI GGNCC 1 cut(s) 596
PspXI VCTCGAGB 1 cut(s) 1225
RsaI GTAC 3 cut(s) 931, 1267, 1286
RsaNI GTAC 3 cut(s) 930, 1266, 1285
RseI CAYNNNNRTG 2 cut(s) 1634, 1705
SacI GAGCTC 1 cut(s) 304
SaqAI TTAA 3 cut(s) 548, 903, 1337
Sau96I GGNCC 1 cut(s) 596
ScaI AGTACT 1 cut(s) 1267
SchI GAGTC 2 cut(s) 1012, 1245
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 3 cut(s) 304, 380, 687
SfaNI GCATC 6 cut(s) 73, 437, 499, 544, 1047, 1638
SfcI CTRYAG 2 cut(s) 179, 1703
Sfr274I CTCGAG 2 cut(s) 297, 1225
SinI GGWCC 1 cut(s) 596
SlaI CTCGAG 2 cut(s) 297, 1225
SmiMI CAYNNNNRTG 2 cut(s) 1634, 1705
SmlI CTYRAG 6 cut(s) 218, 297, 469, 547, 1225, 1291
SmoI CTYRAG 6 cut(s) 218, 297, 469, 547, 1225, 1291
SpeI ACTAGT 1 cut(s) 1210
Sse9I AATT 9 cut(s) 736, 745, 797, 1017, 1051, 1174, 1471, 1498, 1510
SspMI CTAG 5 cut(s) 243, 339, 435, 1211, 1434
SstI GAGCTC 1 cut(s) 304
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 4 cut(s) 209, 988, 1536, 1585
TaaI ACNGT 5 cut(s) 455, 482, 1265, 1284, 1707
TaiI ACGT 2 cut(s) 428, 648
TaqI TCGA 6 cut(s) 298, 881, 1055, 1130, 1226, 1367
TasI AATT 9 cut(s) 736, 745, 797, 1017, 1051, 1174, 1471, 1498, 1510
TatI WGTACW 1 cut(s) 1265
TauI GCSGC 4 cut(s) 13, 669, 1074, 1422
Tru1I TTAA 3 cut(s) 548, 903, 1337
Tru9I TTAA 3 cut(s) 548, 903, 1337
TscAI CASTG 2 cut(s) 519, 1102
TseFI GTSAC 1 cut(s) 1299
TseI GCWGC 7 cut(s) 13, 669, 867, 890, 939, 1115, 1190
Tsp45I GTSAC 1 cut(s) 1299
TspDTI ATGAA 5 cut(s) 124, 742, 1332, 1355, 1589
TspGWI ACGGA 1 cut(s) 696
TspRI CASTG 2 cut(s) 519, 1102
Vha464I CTTAAG 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 596
VspI ATTAAT 1 cut(s) 1337
XapI RAATTY 3 cut(s) 1051, 1498, 1510
XbaI TCTAGA 1 cut(s) 338
XceI RCATGY 1 cut(s) 1090
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XhoI CTCGAG 2 cut(s) 297, 1225
XspI CTAG 5 cut(s) 243, 339, 435, 1211, 1434
ZrmI AGTACT 1 cut(s) 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.