MD13G1018100.v1.1

Vacuolar fusion protein CCZ1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005810
Physical Location & Seq
Forward (+)
1158310 .. 1162011
3702 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1018100.v1.1.491

Sequence Viewer

Length: 1539 bp
ATGGGCTTGTCGTCTGCTACTACTACGGCTACCAATCCAGAGGGAGTGAAATTGTGCGTATTCGATTTGAGGAGGGGTCAGAACGAAGGGCAGGAGTTGGACAAGATACTCTTCTTCTTTCCAGCCGATTTGCCCTTCTCCGCCCAATTCTCTGTGATCGGGCTCAGTGAAGGACTCATCACATTTACAAGAATCTTCTCTCCGGAGGCTGCTTGTGAGGCCATTGAAGCAGAGAGGCATTCCCATGTCTTTTACGAGGCGGAACCTGATATCTGGATGGTCATGGTAGTGGAGAAAAACAAGGAGTTTGAACCGATTTGGAGAAGTGATGCATTGAGAAAGGTTCTCAAGGAAGTACATTCTCTTTTCGTGATGTTTCATGGATCGATAAGAGCGTTGCTTGATAAAGACCCCGGTGGAGGACTAACTCGACCTCATTTGTACCATTTCATCATGGACTATCTCAGTGATTTTCTGGTTGGGAAGAAACTACTGCTACCCTCATTCCGTGACTCTTTGAAGGAGCGTGGAACTGTACAGATGCTTACTGTAGGGAGGGAAGCAGCGATCGAAGTTCAGTCACTTGTTAGGGTACTTGAATCTTGTGCTGGGAACATGCCATGTCACTCATTAATCTTGTTCCAGGACCTGTTGGTGTCCACGACACTTTCTCCTGATGATACGGTAAACCTCTTTGCATATGCTGTTTTAAGGTTGACTCCCCGTGCTTTATCCTCTGGAGGAAGTTCCTGGTCCTATTTACGGAAGGGAACTACATCCTCAAGCTCTAGTTTGGTCAATCCTGGTGCTGTTCCAGAACAATTGCATGGCTCACTTGACAACTCTCCTGCTGGAGATAACAGTTCTCGTGTTGTACGGCCCTTGCAGCCTGATAAGTGGTCCAAAGGAAAGGACGGTTTTCTTGTCAGTGATATTTGGGGTGCAGAGGCTAGTACCGGAGCTTCTGCCACTCCAACAGTTTTGCTCCACCAGACAGAGGAGAGAATGTATCTGTGTACCCATCAGCATAAGAGCCTTACCTTAATTTTTCTCCTTCCTGTTTCTTCGGTACTAAACGGTGATCAAGGTGTTTCTGCAGTGAAGCAGCAAGTTCTTGAAAACGTATCCCTTAAGCTGTTGAAAGTTGAAGAGAAATTATCCAAAGGATGGGCTGGTGAGAATGCGTATCATGTTAGTGGATATCGGTATTTACTTGTGGATGGAGACAGAAATGTATCCAGGGCTTCTCCACCGGGAAAAGTCACAACCCTTACCAAGGACTCGTTACTTGCCTTAAGTAAGGTCAGAGAAGAAGTTGATTTGGAAAAAAGTAGAGCGAAATCGGATAATGCTGGTCATGAGACAGAATTGGAAGTTAGCATCAGAGCGAAAAACAATGCTTGGGTTATTGCTCGGGTTACAAGAGGGAAGGAGCTTTATATGGTTCTAGAGAAAGCCAATGAAACACTTCTCTATGCCTCTGATGCTGTTGAGAAGTTCAGCAACAGGTACTGCAATGGAGCTTTCTCGTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0031982 GO:0043226 GO:0043227 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1712

Protein Analysis

513

Amino Acids

56.51

Weight (kDa)

6.26

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intu_longin_1 PF19031 19 - 128 1.8e-19 First Longin domain of INTU, CCZ1 and HPS4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1167
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 2 cut(s) 141, 260
AclWI GGATC 1 cut(s) 391
AfaI GTAC 9 cut(s) 357, 443, 537, 594, 876, 955, 1018, 1071, 1511
AfiI CCNNNNNNNGG 5 cut(s) 419, 762, 997, 1167, 1276
AflII CTTAAG 2 cut(s) 1130, 1294
AgsI TTSAA 8 cut(s) 227, 311, 520, 599, 1118, 1141, 1148, 1534
AjnI CCWGG 4 cut(s) 642, 749, 802, 1238
AluBI AGCT 5 cut(s) 786, 962, 1135, 1435, 1523
AluI AGCT 5 cut(s) 786, 962, 1135, 1435, 1523
Alw26I GTCTC 2 cut(s) 1218, 1355
AlwI GGATC 1 cut(s) 391
AlwNI CAGNNNCTG 2 cut(s) 649, 1512
Ama87I CYCGRG 1 cut(s) 1413
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 2 cut(s) 219, 878
ApeKI GCWGC 4 cut(s) 209, 563, 886, 1105
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 1467
AspS9I GGNCC 4 cut(s) 646, 753, 879, 900
AsuC2I CCSGG 2 cut(s) 414, 1254
AsuHPI GGTGA 2 cut(s) 1091, 1187
AvaI CYCGRG 1 cut(s) 1413
AvaII GGWCC 3 cut(s) 646, 753, 900
BanII GRGCYC 1 cut(s) 165
BarI GAAGNNNNNNTAC 2 cut(s) 946, 978
BauI CACGAG 1 cut(s) 867
BbvI GCAGC 4 cut(s) 196, 575, 898, 1117
BccI CCATC 4 cut(s) 271, 1029, 1161, 1214
BceAI ACGGC 2 cut(s) 42, 893
BciT130I CCWGG 4 cut(s) 644, 751, 804, 1240
BciVI GTATCC 2 cut(s) 1135, 1246
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 2 cut(s) 414, 1254
BcoDI GTCTC 2 cut(s) 1218, 1355
BfaI CTAG 3 cut(s) 789, 951, 1448
BfmI CTRYAG 2 cut(s) 549, 1095
BfrI CTTAAG 2 cut(s) 1130, 1294
BfuI GTATCC 2 cut(s) 1135, 1246
BisI GCNGC 4 cut(s) 210, 564, 887, 1106
BlsI GCNGC 4 cut(s) 211, 565, 888, 1107
Bme1390I CCNGG 6 cut(s) 414, 644, 751, 804, 1240, 1254
Bme18I GGWCC 3 cut(s) 646, 753, 900
BmeT110I CYCGRG 1 cut(s) 1413
BmgT120I GGNCC 4 cut(s) 646, 753, 879, 900
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 6 cut(s) 414, 644, 751, 804, 1240, 1254
BmsI GCATC 4 cut(s) 319, 531, 1389, 1474
BpmI CTGGAG 2 cut(s) 759, 873
BpuEI CTTGAG 2 cut(s) 332, 766
BpuMI CCSGG 2 cut(s) 414, 1254
Bsa29I ATCGAT 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 412, 1239, 1275
BsaWI WCCGGW 2 cut(s) 202, 956
BsaXI ACNNNNNCTCC 4 cut(s) 655, 685, 992, 1022
Bsc4I CCNNNNNNNGG 5 cut(s) 419, 762, 997, 1167, 1276
Bse3DI GCAATG 1 cut(s) 1522
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 4 cut(s) 644, 751, 804, 1240
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 412, 1239, 1275
BseGI GGATG 4 cut(s) 282, 776, 1172, 1225
BseLI CCNNNNNNNGG 5 cut(s) 419, 762, 997, 1167, 1276
BseMI GCAATG 1 cut(s) 1522
BseMII CTCAG 2 cut(s) 178, 478
BseRI GAGGAG 2 cut(s) 85, 1013
BseXI GCAGC 4 cut(s) 196, 575, 898, 1117
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 963
Bsh1285I CGRYCG 1 cut(s) 570
BshFI GGCC 2 cut(s) 221, 880
BshVI ATCGAT 1 cut(s) 386
BsiEI CGRYCG 1 cut(s) 570
BsiHKCI CYCGRG 1 cut(s) 1413
BsiSI CCGG 4 cut(s) 203, 414, 957, 1253
BslI CCNNNNNNNGG 5 cut(s) 419, 762, 997, 1167, 1276
BsmAI GTCTC 2 cut(s) 1218, 1355
BsmI GAATGC 2 cut(s) 238, 1186
BsnI GGCC 2 cut(s) 221, 880
BsoBI CYCGRG 1 cut(s) 1413
Bsp1286I GDGCHC 1 cut(s) 165
Bsp13I TCCGGA 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 535
Bsp143I GATC 4 cut(s) 156, 383, 567, 1081
BspACI CCGC 2 cut(s) 141, 260
BspANI GGCC 2 cut(s) 221, 880
BspCNI CTCAG 2 cut(s) 177, 477
BspDI ATCGAT 1 cut(s) 386
BspEI TCCGGA 1 cut(s) 202
BspHI TCATGA 1 cut(s) 1357
BspLI GGNNCC 1 cut(s) 264
BspMAI CTGCAG 1 cut(s) 1099
BspPI GGATC 1 cut(s) 391
BspTI CTTAAG 2 cut(s) 1130, 1294
BsrDI GCAATG 1 cut(s) 1522
BsrGI TGTACA 1 cut(s) 535
BssECI CCNNGG 3 cut(s) 412, 1239, 1275
BssMI GATC 4 cut(s) 156, 383, 567, 1081
BssSI CACGAG 1 cut(s) 867
BssT1I CCWWGG 1 cut(s) 1275
Bst2BI CACGAG 1 cut(s) 867
Bst2UI CCWGG 4 cut(s) 644, 751, 804, 1240
Bst4CI ACNGT 7 cut(s) 535, 550, 685, 863, 917, 979, 1079
Bst6I CTCTTC 2 cut(s) 116, 1143
BstAFI CTTAAG 2 cut(s) 1130, 1294
BstAUI TGTACA 1 cut(s) 535
BstDEI CTNAG 2 cut(s) 164, 464
BstENI CCTNNNNNAGG 1 cut(s) 1274
BstF5I GGATG 4 cut(s) 282, 776, 1172, 1225
BstKTI GATC 4 cut(s) 159, 386, 570, 1084
BstMAI GTCTC 2 cut(s) 1218, 1355
BstMBI GATC 4 cut(s) 156, 383, 567, 1081
BstMCI CGRYCG 1 cut(s) 570
BstMWI GCNNNNNNNGC 4 cut(s) 218, 227, 886, 1484
BstNI CCWGG 4 cut(s) 644, 751, 804, 1240
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 6 cut(s) 412, 642, 749, 802, 1238, 1252
BstSFI CTRYAG 2 cut(s) 549, 1095
BstV1I GCAGC 4 cut(s) 196, 575, 898, 1117
Bsu15I ATCGAT 1 cut(s) 386
BsuI GTATCC 2 cut(s) 1135, 1246
BsuRI GGCC 2 cut(s) 221, 880
BsuTUI ATCGAT 1 cut(s) 386
BtsCI GGATG 4 cut(s) 282, 776, 1172, 1225
BtsI GCAGTG 1 cut(s) 1104
BtsIMutI CAGTG 4 cut(s) 172, 472, 934, 1104
CaiI CAGNNNCTG 2 cut(s) 649, 1512
CciI TCATGA 1 cut(s) 1357
Cfr13I GGNCC 4 cut(s) 646, 753, 879, 900
ClaI ATCGAT 1 cut(s) 386
Csp6I GTAC 9 cut(s) 356, 442, 536, 593, 875, 954, 1017, 1070, 1510
CviAII CATG 9 cut(s) 245, 283, 380, 454, 616, 621, 827, 1190, 1358
CviQI GTAC 9 cut(s) 356, 442, 536, 593, 875, 954, 1017, 1070, 1510
DdeI CTNAG 2 cut(s) 164, 464
DpnI GATC 4 cut(s) 158, 385, 569, 1083
DpnII GATC 4 cut(s) 156, 383, 567, 1081
Eam1104I CTCTTC 2 cut(s) 116, 1143
EarI CTCTTC 2 cut(s) 116, 1143
EciI GGCGGA 2 cut(s) 130, 275
Eco130I CCWWGG 1 cut(s) 1275
Eco24I GRGCYC 1 cut(s) 165
Eco32I GATATC 2 cut(s) 271, 1202
Eco47I GGWCC 3 cut(s) 646, 753, 900
Eco88I CYCGRG 1 cut(s) 1413
EcoNI CCTNNNNNAGG 1 cut(s) 1274
EcoO109I RGGNCCY 1 cut(s) 646
EcoRII CCWGG 4 cut(s) 642, 749, 802, 1238
EcoRV GATATC 2 cut(s) 271, 1202
EcoT14I CCWWGG 1 cut(s) 1275
EcoT22I ATGCAT 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 1275
FaeI CATG 9 cut(s) 248, 286, 383, 457, 619, 624, 830, 1193, 1361
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FatI CATG 9 cut(s) 244, 282, 379, 453, 615, 620, 826, 1189, 1357
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 1081
Fnu4HI GCNGC 4 cut(s) 210, 564, 887, 1106
FokI GGATG 4 cut(s) 289, 763, 1179, 1232
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 4 cut(s) 210, 564, 887, 1106
FspBI CTAG 3 cut(s) 789, 951, 1448
GluI GCNGC 4 cut(s) 210, 564, 887, 1106
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 2 cut(s) 759, 873
HaeIII GGCC 2 cut(s) 221, 880
HapII CCGG 4 cut(s) 203, 414, 957, 1253
Hin1II CATG 9 cut(s) 248, 286, 383, 457, 619, 624, 830, 1193, 1361
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 6 cut(s) 174, 192, 512, 599, 718, 1280
HpaII CCGG 4 cut(s) 203, 414, 957, 1253
HphI GGTGA 2 cut(s) 1091, 1187
Hpy166II GTNNAC 4 cut(s) 660, 688, 717, 1017
Hpy188I TCNGA 5 cut(s) 81, 1307, 1345, 1385, 1483
Hpy8I GTNNAC 4 cut(s) 660, 688, 717, 1017
HpyAV CCTTC 7 cut(s) 80, 145, 164, 514, 760, 1064, 1423
HpyCH4III ACNGT 7 cut(s) 535, 550, 685, 863, 917, 979, 1079
HpyCH4IV ACGT 1 cut(s) 1122
HpyCH4V TGCA 7 cut(s) 332, 698, 826, 886, 944, 1097, 1515
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 227, 886, 1484
HpyF3I CTNAG 2 cut(s) 164, 464
HpySE526I ACGT 1 cut(s) 1122
Hsp92II CATG 9 cut(s) 248, 286, 383, 457, 619, 624, 830, 1193, 1361
Kpn2I TCCGGA 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 4 cut(s) 156, 383, 567, 1081
LmnI GCTCC 5 cut(s) 523, 959, 990, 1432, 1520
Lsp1109I GCAGC 4 cut(s) 196, 575, 898, 1117
LweI GCATC 4 cut(s) 319, 531, 1389, 1474
MaeI CTAG 3 cut(s) 789, 951, 1448
MaeII ACGT 1 cut(s) 1122
MaeIII GTNAC 6 cut(s) 509, 579, 623, 1261, 1284, 1417
MalI GATC 4 cut(s) 158, 385, 569, 1083
MboI GATC 4 cut(s) 156, 383, 567, 1081
MboII GAAGA 7 cut(s) 103, 106, 187, 496, 1056, 1160, 1322
MfeI CAATTG 1 cut(s) 821
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 7 cut(s) 50, 146, 821, 1044, 1154, 1367, 1534
MlyI GAGTC 4 cut(s) 168, 506, 712, 1274
MmeI TCCRAC 2 cut(s) 78, 998
Mph1103I ATGCAT 1 cut(s) 334
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 1467
MseI TTAA 5 cut(s) 632, 710, 1043, 1131, 1295
MslI CAYNNNNRTG 3 cut(s) 243, 287, 1194
MspCI CTTAAG 2 cut(s) 1130, 1294
MspI CCGG 4 cut(s) 203, 414, 957, 1253
MspR9I CCNGG 6 cut(s) 414, 644, 751, 804, 1240, 1254
MunI CAATTG 1 cut(s) 821
Mva1269I GAATGC 2 cut(s) 238, 1186
MvaI CCWGG 4 cut(s) 644, 751, 804, 1240
MwoI GCNNNNNNNGC 4 cut(s) 218, 227, 886, 1484
NciI CCSGG 2 cut(s) 414, 1254
NdeI CATATG 1 cut(s) 700
NdeII GATC 4 cut(s) 156, 383, 567, 1081
NlaIII CATG 9 cut(s) 248, 286, 383, 457, 619, 624, 830, 1193, 1361
NlaIV GGNNCC 1 cut(s) 264
NmuCI GTSAC 4 cut(s) 509, 579, 623, 1261
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 1 cut(s) 619
PagI TCATGA 1 cut(s) 1357
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PctI GAATGC 2 cut(s) 238, 1186
PdmI GAANNNNTTC 1 cut(s) 1467
PfeI GAWTC 2 cut(s) 192, 599
PflMI CCANNNNNTGG 1 cut(s) 1167
PfoI TCCNGGA 1 cut(s) 642
PkrI GCNGC 4 cut(s) 211, 565, 888, 1107
Ple19I CGATCG 1 cut(s) 570
PleI GAGTC 4 cut(s) 168, 506, 712, 1274
PpsI GAGTC 4 cut(s) 168, 506, 712, 1274
PpuMI RGGWCCY 1 cut(s) 646
PshBI ATTAAT 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 4 cut(s) 642, 749, 802, 1238
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 4 cut(s) 642, 749, 802, 1238
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 4 cut(s) 646, 753, 879, 900
PspPPI RGGWCCY 1 cut(s) 646
PstI CTGCAG 1 cut(s) 1099
PstNI CAGNNNCTG 2 cut(s) 649, 1512
PvuI CGATCG 1 cut(s) 570
RsaI GTAC 9 cut(s) 357, 443, 537, 594, 876, 955, 1018, 1071, 1511
RsaNI GTAC 9 cut(s) 356, 442, 536, 593, 875, 954, 1017, 1070, 1510
RseI CAYNNNNRTG 3 cut(s) 243, 287, 1194
SaqAI TTAA 5 cut(s) 632, 710, 1043, 1131, 1295
SatI GCNGC 4 cut(s) 210, 564, 887, 1106
Sau3AI GATC 4 cut(s) 156, 383, 567, 1081
Sau96I GGNCC 4 cut(s) 646, 753, 879, 900
SchI GAGTC 4 cut(s) 168, 506, 712, 1274
ScrFI CCNGG 6 cut(s) 414, 644, 751, 804, 1240, 1254
SduI GDGCHC 1 cut(s) 165
SfaNI GCATC 4 cut(s) 319, 531, 1389, 1474
SfcI CTRYAG 2 cut(s) 549, 1095
SinI GGWCC 3 cut(s) 646, 753, 900
SmiMI CAYNNNNRTG 3 cut(s) 243, 287, 1194
SmlI CTYRAG 4 cut(s) 347, 781, 1130, 1294
SmoI CTYRAG 4 cut(s) 347, 781, 1130, 1294
Sse9I AATT 7 cut(s) 50, 146, 821, 1044, 1154, 1367, 1534
SsiI CCGC 2 cut(s) 141, 260
SspMI CTAG 3 cut(s) 789, 951, 1448
StyD4I CCNGG 6 cut(s) 412, 642, 749, 802, 1238, 1252
StyI CCWWGG 1 cut(s) 1275
TaaI ACNGT 7 cut(s) 535, 550, 685, 863, 917, 979, 1079
TaiI ACGT 1 cut(s) 1125
TaqI TCGA 4 cut(s) 63, 386, 430, 570
TasI AATT 7 cut(s) 50, 146, 821, 1044, 1154, 1367, 1534
TatI WGTACW 2 cut(s) 355, 535
TfiI GAWTC 2 cut(s) 192, 599
Tru1I TTAA 5 cut(s) 632, 710, 1043, 1131, 1295
Tru9I TTAA 5 cut(s) 632, 710, 1043, 1131, 1295
TscAI CASTG 4 cut(s) 172, 472, 934, 1104
TseFI GTSAC 4 cut(s) 509, 579, 623, 1261
TseI GCWGC 4 cut(s) 209, 563, 886, 1105
Tsp45I GTSAC 4 cut(s) 509, 579, 623, 1261
TspDTI ATGAA 3 cut(s) 368, 439, 1476
TspGWI ACGGA 2 cut(s) 497, 778
TspRI CASTG 4 cut(s) 172, 472, 934, 1104
Van91I CCANNNNNTGG 1 cut(s) 1167
Vha464I CTTAAG 2 cut(s) 1130, 1294
VpaK11BI GGWCC 3 cut(s) 646, 753, 900
VspI ATTAAT 1 cut(s) 632
XagI CCTNNNNNAGG 1 cut(s) 1274
XbaI TCTAGA 1 cut(s) 1447
XceI RCATGY 1 cut(s) 619
XmnI GAANNNNTTC 1 cut(s) 1467
XspI CTAG 3 cut(s) 789, 951, 1448
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.