RchiOBHm_Chr4g0442431

Vacuolar fusion protein CCZ1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005810
Physical Location & Seq
Forward (+)
64010465 .. 64014437
3973 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41019

Sequence Viewer

Length: 1548 bp
ATGGGTTTGGCATCCGCTAGTACTGCCACTACTGGCCCAGAAGCTATGCAATTGTGCATATTTGATTTGCGGAGGGGTCAAAACGAAGGCCAAGAGTTGGATAAGATACTGTTTTTCTATCCTGCTGATTTGCCTTTCTCAGCTCAGTTCTCTGTGATTGGGCTCAGTGAAGGACTCATCACATTTACCAGAATCTTCTCTCCGGAGGCGGCTTGTGAGGCCATAGAAGCAGATAGGCATTCACATGTTTTTTACGAGGCCGAACCCGATATCTGGATGGTTATGGTAGTGGAGAAAAGCATGGAGTACGAAGCGAAATGGAGAACTGATGCATTAAGAAAGGTTCTTAAGGAAGTGCATTCTCTTTTTGTAATGTTTCATGGTTCTGTAAGAGCGATGCTTGATAAAGACCCGGGGGGAGTGCTAGCTAGATCTCATTTGTACTCTTTCGTCATGGATTATCTTGGTGATTTTCTTGTTGGGAAGAAGCTACTGATGCCATCATTCCGTGACTCTTTAAAGGAGCGTGGAACTGTGCAGATGCTTACTGTAGGGCGGGAAGCCGCAATTGAAGTTCAGTCACTTGTCAGGGTACTTGAATCTAGTGCTGGGAACTTGCCCTGTCATTCACTAATCTTGTTTCAGGACCTGTTGGTATCCACAACACTTTCTCCTGATGACACCGTAAACCTATTTGCATATGCTGTTTTAAGGTTGACACCCCATGTTTTGTCCTCTGGAGCAAGTTCCTGGTCTTATTTACGCAAAGGAGCTACAGCTAACGTTTCCTCAAGTTCTATCTTGTCCGATTCTGGTACTGTTCCTGAGCAGTATCATGGCTCACACAACAATTCTTCTGCTGGAGACAACAGTTCTCGCGTCATACGGCCATTGAAGCTGGACAAGTGGTCCAAAGGGAACGATGGTTTTCTTGCCACTGATATTTGGGGTGCAGAGCCTAGTACCGGGGTGTCGGCCACTCCAACAGTATTTCTCCATCAGTCAGAGGAAAGGATGTATCTGTGTGCCCATCAGCATAAAAGCCTCACCTTACTTTTTCTCATTCCTGTTTCTTCAGTACTGAATGGGGAGCAAGGTCTTTCTACAGTGAAGCAGCAAGTTCTTGAGAATGTATCCCTAAAGCTGTTGAAAGTAGAAGAAAAACTATCAAAAGGATGGGGCGGTGAGAACGCATATCATGTTAGTGGATATCGGTATTTACTAGTGGATGGAGACAGAAACGTATCCAGGGCTTCTCCAACAGGAAAGGTCACAACGCTTAGTAAGGAGTCCTTACTTGCCTTAAGTAAGCTTAGAGAAGAAGTTGATAGAGAAAAGAGTAGAGCACAGTGGGATAGCCCCGGTCATGAAAAAGAGTTGGAAGTTAGCATCAGGGCAAAAAACAATGCTTGGGTTATTGCTCGGGTAACAAGAGGGAAGGAGCTTTACATGGTTCTGGAAAAAGCCAATGAAACACTTCTTTACGCCTCCGATGCTGTTGAAAAGTTCAGCAACAGGTACTGCGGCGGAGCTTTCTCGTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0031982 GO:0043226 GO:0043227 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1712

Protein Analysis

515

Amino Acids

56.98

Weight (kDa)

6.19

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intu_longin_1 PF19031 19 - 128 1.2e-19 First Longin domain of INTU, CCZ1 and HPS4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 97
AccII CGCG 1 cut(s) 879
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 8 cut(s) 15, 70, 209, 556, 564, 1182, 1524, 1527
AclI AACGTT 1 cut(s) 783
AcoI YGGCCR 2 cut(s) 887, 975
AcuI CTGAAG 1 cut(s) 1059
AfaI GTAC 8 cut(s) 22, 308, 443, 594, 817, 964, 1080, 1520
AfiI CCNNNNNNNGG 3 cut(s) 97, 273, 965
AflII CTTAAG 2 cut(s) 347, 1303
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 6 cut(s) 572, 599, 895, 1150, 1502, 1543
AhdI GACNNNNNGTC 1 cut(s) 907
AhlI ACTAGT 1 cut(s) 1222
AjnI CCWGG 2 cut(s) 749, 1247
Alw21I GWGCWC 1 cut(s) 1348
Alw26I GTCTC 2 cut(s) 858, 1227
AlwNI CAGNNNCTG 2 cut(s) 649, 1521
Ama87I CYCGRG 2 cut(s) 412, 1422
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 6 cut(s) 34, 88, 219, 258, 887, 975
ApeKI GCWGC 1 cut(s) 1114
Asp700I GAANNNNTTC 1 cut(s) 1476
AspS9I GGNCC 3 cut(s) 35, 646, 909
AsuC2I CCSGG 4 cut(s) 413, 414, 967, 1362
AsuHPI GGTGA 3 cut(s) 479, 1039, 1196
AsuNHI GCTAGC 1 cut(s) 424
AvaI CYCGRG 2 cut(s) 412, 1422
AvaII GGWCC 2 cut(s) 646, 909
BaeGI GKGCMC 1 cut(s) 1030
BanII GRGCYC 1 cut(s) 165
BarI GAAGNNNNNNTAC 2 cut(s) 1430, 1462
Bbv12I GWGCWC 1 cut(s) 1348
BbvI GCAGC 1 cut(s) 1126
BccI CCATC 7 cut(s) 271, 508, 917, 1005, 1038, 1170, 1223
BceAI ACGGC 1 cut(s) 902
BciT130I CCWGG 2 cut(s) 751, 1249
BciVI GTATCC 3 cut(s) 667, 1144, 1255
BcnI CCSGG 4 cut(s) 413, 414, 967, 1362
BcoDI GTCTC 2 cut(s) 858, 1227
BcuI ACTAGT 1 cut(s) 1222
BfaI CTAG 6 cut(s) 18, 425, 429, 603, 960, 1223
BfmI CTRYAG 3 cut(s) 549, 774, 1104
BfrI CTTAAG 2 cut(s) 347, 1303
BfuI GTATCC 3 cut(s) 667, 1144, 1255
BglII AGATCT 1 cut(s) 431
BisI GCNGC 4 cut(s) 210, 564, 1115, 1525
BlsI GCNGC 4 cut(s) 211, 565, 1116, 1526
BmcAI AGTACT 2 cut(s) 22, 1080
Bme1390I CCNGG 6 cut(s) 413, 414, 751, 967, 1249, 1362
Bme18I GGWCC 2 cut(s) 646, 909
BmeRI GACNNNNNGTC 1 cut(s) 907
BmeT110I CYCGRG 2 cut(s) 412, 1422
BmgT120I GGNCC 3 cut(s) 35, 646, 909
BmrFI CCNGG 6 cut(s) 413, 414, 751, 967, 1249, 1362
BmsI GCATC 7 cut(s) 20, 319, 387, 486, 531, 1398, 1483
BmtI GCTAGC 1 cut(s) 428
BpmI CTGGAG 2 cut(s) 759, 882
Bpu10I CCTNAGC 1 cut(s) 825
BpuEI CTTGAG 2 cut(s) 775, 1145
BpuMI CCSGG 4 cut(s) 413, 414, 967, 1362
BsaJI CCNNGG 5 cut(s) 412, 413, 966, 1248, 1360
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 273, 965
Bse1I ACTGG 1 cut(s) 37
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 2 cut(s) 751, 1249
BseDI CCNNGG 5 cut(s) 412, 413, 966, 1248, 1360
BseGI GGATG 5 cut(s) 11, 282, 1020, 1181, 1234
BseLI CCNNNNNNNGG 3 cut(s) 97, 273, 965
BseMII CTCAG 4 cut(s) 153, 158, 178, 816
BseNI ACTGG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 1 cut(s) 1126
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 2 cut(s) 557, 972
Bsh1236I CGCG 1 cut(s) 879
BshFI GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
BsiHKAI GWGCWC 1 cut(s) 1348
BsiHKCI CYCGRG 2 cut(s) 412, 1422
BsiSI CCGG 4 cut(s) 203, 413, 966, 1362
BslI CCNNNNNNNGG 3 cut(s) 97, 273, 965
BsmAI GTCTC 2 cut(s) 858, 1227
BsmI GAATGC 2 cut(s) 238, 358
BsnI GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
BsoBI CYCGRG 2 cut(s) 412, 1422
Bsp1286I GDGCHC 3 cut(s) 165, 1030, 1348
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 1 cut(s) 431
BspACI CCGC 8 cut(s) 15, 70, 209, 556, 564, 1182, 1524, 1527
BspANI GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
BspCNI CTCAG 4 cut(s) 152, 157, 177, 817
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 1 cut(s) 879
BspHI TCATGA 1 cut(s) 1366
BspOI GCTAGC 1 cut(s) 428
BspTI CTTAAG 2 cut(s) 347, 1303
BsrI ACTGG 1 cut(s) 37
BssECI CCNNGG 5 cut(s) 412, 413, 966, 1248, 1360
BssMI GATC 1 cut(s) 431
Bst2UI CCWGG 2 cut(s) 751, 1249
Bst4CI ACNGT 9 cut(s) 111, 535, 550, 685, 820, 872, 988, 1108, 1350
BstAFI CTTAAG 2 cut(s) 347, 1303
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 6 cut(s) 139, 144, 164, 825, 1280, 1313
BstF5I GGATG 5 cut(s) 11, 282, 1020, 1181, 1234
BstFNI CGCG 1 cut(s) 879
BstKTI GATC 1 cut(s) 434
BstMAI GTCTC 2 cut(s) 858, 1227
BstMBI GATC 1 cut(s) 431
BstMWI GCNNNNNNNGC 6 cut(s) 23, 218, 227, 496, 895, 1493
BstNI CCWGG 2 cut(s) 751, 1249
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 6 cut(s) 411, 412, 749, 965, 1247, 1360
BstSFI CTRYAG 3 cut(s) 549, 774, 1104
BstSLI GKGCMC 1 cut(s) 1030
BstUI CGCG 1 cut(s) 879
BstV1I GCAGC 1 cut(s) 1126
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BsuI GTATCC 3 cut(s) 667, 1144, 1255
BsuRI GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
BtgZI GCGATG 1 cut(s) 410
BtsCI GGATG 5 cut(s) 11, 282, 1020, 1181, 1234
BtsIMutI CAGTG 4 cut(s) 172, 936, 1113, 1355
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 2 cut(s) 649, 1521
CciI TCATGA 1 cut(s) 1366
Cfr13I GGNCC 3 cut(s) 35, 646, 909
Cfr9I CCCGGG 1 cut(s) 412
CseI GACGC 1 cut(s) 868
Csp6I GTAC 8 cut(s) 21, 307, 442, 593, 816, 963, 1079, 1519
CviAII CATG 9 cut(s) 245, 301, 380, 454, 725, 836, 1199, 1367, 1450
CviQI GTAC 8 cut(s) 21, 307, 442, 593, 816, 963, 1079, 1519
DdeI CTNAG 6 cut(s) 139, 144, 164, 825, 1280, 1313
DpnI GATC 1 cut(s) 433
DpnII GATC 1 cut(s) 431
DraI TTTAAA 1 cut(s) 519
DriI GACNNNNNGTC 1 cut(s) 907
EaeI YGGCCR 2 cut(s) 887, 975
Eam1105I GACNNNNNGTC 1 cut(s) 907
EciI GGCGGA 1 cut(s) 1542
Eco24I GRGCYC 1 cut(s) 165
Eco32I GATATC 2 cut(s) 271, 1211
Eco47I GGWCC 2 cut(s) 646, 909
Eco57I CTGAAG 1 cut(s) 1059
Eco88I CYCGRG 2 cut(s) 412, 1422
EcoO109I RGGNCCY 1 cut(s) 646
EcoRII CCWGG 2 cut(s) 749, 1247
EcoRV GATATC 2 cut(s) 271, 1211
EcoT22I ATGCAT 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 165
FaeI CATG 9 cut(s) 248, 304, 383, 457, 728, 839, 1202, 1370, 1453
FalI AAGNNNNNCTT 2 cut(s) 1277, 1309
FatI CATG 9 cut(s) 244, 300, 379, 453, 724, 835, 1198, 1366, 1449
FauI CCCGC 1 cut(s) 549
FauNDI CATATG 1 cut(s) 700
Fnu4HI GCNGC 4 cut(s) 210, 564, 1115, 1525
FokI GGATG 4 cut(s) 289, 1027, 1188, 1241
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 4 cut(s) 210, 564, 1115, 1525
FspBI CTAG 6 cut(s) 18, 425, 429, 603, 960, 1223
GluI GCNGC 4 cut(s) 210, 564, 1115, 1525
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 2 cut(s) 759, 882
HaeIII GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
HapII CCGG 4 cut(s) 203, 413, 966, 1362
HgaI GACGC 1 cut(s) 868
Hin1II CATG 9 cut(s) 248, 304, 383, 457, 728, 839, 1202, 1370, 1453
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HindIII AAGCTT 1 cut(s) 1310
HinfI GANTC 6 cut(s) 174, 192, 512, 599, 809, 1289
HpaII CCGG 4 cut(s) 203, 413, 966, 1362
HphI GGTGA 3 cut(s) 479, 1039, 1196
Hpy166II GTNNAC 2 cut(s) 688, 717
Hpy188I TCNGA 3 cut(s) 808, 1006, 1492
Hpy188III TCNNGA 9 cut(s) 203, 274, 644, 674, 738, 824, 1124, 1367, 1457
Hpy8I GTNNAC 2 cut(s) 688, 717
HpyAV CCTTC 3 cut(s) 80, 164, 1432
HpyCH4III ACNGT 9 cut(s) 111, 535, 550, 685, 820, 872, 988, 1108, 1350
HpyCH4IV ACGT 2 cut(s) 783, 1242
HpyCH4V TGCA 7 cut(s) 49, 57, 332, 358, 538, 698, 953
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 218, 227, 496, 895, 1493
HpyF3I CTNAG 6 cut(s) 139, 144, 164, 825, 1280, 1313
HpySE526I ACGT 2 cut(s) 783, 1242
Hsp92II CATG 9 cut(s) 248, 304, 383, 457, 728, 839, 1202, 1370, 1453
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 1 cut(s) 431
LmnI GCTCC 6 cut(s) 523, 740, 770, 1090, 1441, 1529
Lsp1109I GCAGC 1 cut(s) 1126
LweI GCATC 7 cut(s) 20, 319, 387, 486, 531, 1398, 1483
MaeI CTAG 6 cut(s) 18, 425, 429, 603, 960, 1223
MaeII ACGT 2 cut(s) 783, 1242
MaeIII GTNAC 4 cut(s) 509, 579, 1270, 1426
MalI GATC 1 cut(s) 433
MboI GATC 1 cut(s) 431
MboII GAAGA 6 cut(s) 187, 496, 846, 1065, 1169, 1331
MfeI CAATTG 2 cut(s) 50, 567
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 3 cut(s) 165, 1030, 1348
MluCI AATT 4 cut(s) 50, 567, 850, 1543
MlyI GAGTC 3 cut(s) 168, 506, 1298
MmeI TCCRAC 4 cut(s) 78, 1007, 1283, 1359
Mph1103I ATGCAT 1 cut(s) 334
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 1476
MseI TTAA 5 cut(s) 335, 348, 518, 710, 1304
MslI CAYNNNNRTG 2 cut(s) 243, 1203
MspCI CTTAAG 2 cut(s) 347, 1303
MspI CCGG 4 cut(s) 203, 413, 966, 1362
MspR9I CCNGG 6 cut(s) 413, 414, 751, 967, 1249, 1362
MunI CAATTG 2 cut(s) 50, 567
Mva1269I GAATGC 2 cut(s) 238, 358
MvaI CCWGG 2 cut(s) 751, 1249
MvnI CGCG 1 cut(s) 879
MwoI GCNNNNNNNGC 6 cut(s) 23, 218, 227, 496, 895, 1493
NciI CCSGG 4 cut(s) 413, 414, 967, 1362
NdeI CATATG 1 cut(s) 700
NdeII GATC 1 cut(s) 431
NheI GCTAGC 1 cut(s) 424
NlaIII CATG 9 cut(s) 248, 304, 383, 457, 728, 839, 1202, 1370, 1453
NmuCI GTSAC 3 cut(s) 509, 579, 1270
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 1 cut(s) 248
PagI TCATGA 1 cut(s) 1366
PciI ACATGT 1 cut(s) 244
PctI GAATGC 2 cut(s) 238, 358
PdmI GAANNNNTTC 1 cut(s) 1476
PfeI GAWTC 3 cut(s) 192, 599, 809
PflMI CCANNNNNTGG 1 cut(s) 97
PkrI GCNGC 4 cut(s) 211, 565, 1116, 1526
PleI GAGTC 3 cut(s) 168, 506, 1297
PpsI GAGTC 3 cut(s) 168, 506, 1297
PpuMI RGGWCCY 1 cut(s) 646
PscI ACATGT 1 cut(s) 244
Psp1406I AACGTT 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 2 cut(s) 749, 1247
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 2 cut(s) 749, 1247
PspPI GGNCC 3 cut(s) 35, 646, 909
PspPPI RGGWCCY 1 cut(s) 646
PstNI CAGNNNCTG 2 cut(s) 649, 1521
PsuI RGATCY 1 cut(s) 431
RsaI GTAC 8 cut(s) 22, 308, 443, 594, 817, 964, 1080, 1520
RsaNI GTAC 8 cut(s) 21, 307, 442, 593, 816, 963, 1079, 1519
RseI CAYNNNNRTG 2 cut(s) 243, 1203
SaqAI TTAA 5 cut(s) 335, 348, 518, 710, 1304
SatI GCNGC 4 cut(s) 210, 564, 1115, 1525
Sau3AI GATC 1 cut(s) 431
Sau96I GGNCC 3 cut(s) 35, 646, 909
ScaI AGTACT 2 cut(s) 22, 1080
SchI GAGTC 3 cut(s) 168, 506, 1298
ScrFI CCNGG 6 cut(s) 413, 414, 751, 967, 1249, 1362
SduI GDGCHC 3 cut(s) 165, 1030, 1348
SfaNI GCATC 7 cut(s) 20, 319, 387, 486, 531, 1398, 1483
SfcI CTRYAG 3 cut(s) 549, 774, 1104
SinI GGWCC 2 cut(s) 646, 909
SmaI CCCGGG 1 cut(s) 414
SmiMI CAYNNNNRTG 2 cut(s) 243, 1203
SmlI CTYRAG 4 cut(s) 347, 790, 1124, 1303
SmoI CTYRAG 4 cut(s) 347, 790, 1124, 1303
SpeI ACTAGT 1 cut(s) 1222
Sse9I AATT 4 cut(s) 50, 567, 850, 1543
SsiI CCGC 8 cut(s) 15, 70, 209, 556, 564, 1182, 1524, 1527
SspMI CTAG 6 cut(s) 18, 425, 429, 603, 960, 1223
StyD4I CCNGG 6 cut(s) 411, 412, 749, 965, 1247, 1360
TaaI ACNGT 9 cut(s) 111, 535, 550, 685, 820, 872, 988, 1108, 1350
TaiI ACGT 2 cut(s) 786, 1245
TasI AATT 4 cut(s) 50, 567, 850, 1543
TatI WGTACW 3 cut(s) 20, 441, 1078
TauI GCSGC 3 cut(s) 212, 566, 1527
TfiI GAWTC 3 cut(s) 192, 599, 809
Tru1I TTAA 5 cut(s) 335, 348, 518, 710, 1304
Tru9I TTAA 5 cut(s) 335, 348, 518, 710, 1304
TscAI CASTG 4 cut(s) 172, 943, 1113, 1355
TseFI GTSAC 3 cut(s) 509, 579, 1270
TseI GCWGC 1 cut(s) 1114
Tsp45I GTSAC 3 cut(s) 509, 579, 1270
TspDTI ATGAA 3 cut(s) 368, 1383, 1485
TspGWI ACGGA 1 cut(s) 497
TspMI CCCGGG 1 cut(s) 412
TspRI CASTG 4 cut(s) 172, 943, 1113, 1355
Van91I CCANNNNNTGG 1 cut(s) 97
Vha464I CTTAAG 2 cut(s) 347, 1303
VpaK11BI GGWCC 2 cut(s) 646, 909
XceI RCATGY 1 cut(s) 248
XmaI CCCGGG 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 1476
XspI CTAG 6 cut(s) 18, 425, 429, 603, 960, 1223
ZrmI AGTACT 2 cut(s) 22, 1080
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.