Rw4G033930

Vacuolar fusion protein CCZ1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005810
Physical Location & Seq
Forward (+)
61072832 .. 61076879
4048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033930.1

Sequence Viewer

Length: 1140 bp
ATGGGTTTGGCATCCGCTAGTACTGCCACTACTGGCCCAGAAGCTATGCAATTGTGCATATTTGATTTGCGGAGGGGTCAAAACGAAGGCCAAGAGTTGGATAAGATACTGTTTTTCTATCCTGCTGATTTGCCTTTCTCAGCTCAGTTCTCTGTGATTGGGCTCAGTGAAGGACTCATCACATTTACCAGAATCTTCTCTCCGGAGGCGGCTTGTGAGGCCATAGAAGCAGATAGGCATTCACATGTTTTTTACGAGGCCGAACCCGATATCTGGATGGTTATGGTAGTGGAGAAAAGCATGGAGTACGAAGCGAAATGGAGAACTGATGCATTAAGAAAGGTTCTTAAGGAAGTGCATTCTCTTTTTGTAATGTTTCATGGTTCTGTAAGAGCGATGCTTGATAAAGACCCGGGGGGAGTGCTAGCTAGATCTCATTTGTACTCTTTCGTCATGGATTATCTTGGTGATTTTCTTGTGGGGAAGAAGTTACTGATGCCATCATTCCGTGACTCTTTAAAGGAGCGTGGAACTGTGCAGATGCTTACTGTAGGGCGGGAAGCCGCAATTGAAGTTCAGTCACTTGTCAGGGTACTTGAATCTAGTGCTGGGAACTTGCCCTGTCATTCACTAATCTTGTTTCAGGACCTGTTGGTATCCACAACACTTTCTCCTGATGACACCGTAAACCTATTTGCATATGCTGTTTTAAGGTTGACACCCCATGTTTTGTCCTCTGGAGCAAGTTCCTGGTCTTATTTACGCAAAGGAGCTACAGCTAACGTTTCCTCAAGTTCTATCTTGTCCGATTCTGGTACTGTTCCAGAGCAGTATCATGGCTCACACAACAATTCTTCTGCTGGAGACAACAGTTCTCGCGTCATACGGCCATTGAAGCTGGACAAGTGGTCCAAAGGGAACGATGGTTTTCTTGCCACTGATATTTGGGGTGCAGAGCCTAGTACCGGGGTGTCGGCCACTCCAACAGTATTTCTCCATCAGTCAGAGGAAAGGATGTATCTGTGTGCCCATCAGCATAAAAGCCTCACCTTACTTTTTCTCATTCCTGTTTCTTCAGTACTAAATGGGGAGCAAGGTCTTTCTACAGTGAGCAGCAAGTTCTTGAGAATGTATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0031982 GO:0043226 GO:0043227 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1712

Protein Analysis

379

Amino Acids

41.76

Weight (kDa)

5.85

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intu_longin_1 PF19031 19 - 128 6.7e-20 First Longin domain of INTU, CCZ1 and HPS4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 97
AccII CGCG 1 cut(s) 879
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 5 cut(s) 15, 70, 209, 556, 564
AclI AACGTT 1 cut(s) 783
AcoI YGGCCR 2 cut(s) 887, 975
AcuI CTGAAG 1 cut(s) 1059
AfaI GTAC 7 cut(s) 22, 308, 443, 594, 817, 964, 1080
AfiI CCNNNNNNNGG 3 cut(s) 97, 273, 965
AflII CTTAAG 1 cut(s) 347
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 3 cut(s) 572, 599, 895
AhdI GACNNNNNGTC 1 cut(s) 907
AjnI CCWGG 1 cut(s) 749
AluBI AGCT 6 cut(s) 44, 143, 428, 773, 779, 898
AluI AGCT 6 cut(s) 44, 143, 428, 773, 779, 898
Alw26I GTCTC 1 cut(s) 858
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 1 cut(s) 412
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 6 cut(s) 34, 88, 219, 258, 887, 975
ApeKI GCWGC 1 cut(s) 1113
AspS9I GGNCC 3 cut(s) 35, 646, 909
AsuC2I CCSGG 3 cut(s) 413, 414, 967
AsuHPI GGTGA 2 cut(s) 479, 1039
AsuNHI GCTAGC 1 cut(s) 424
AvaI CYCGRG 1 cut(s) 412
AvaII GGWCC 2 cut(s) 646, 909
BaeGI GKGCMC 1 cut(s) 1030
BanII GRGCYC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 1125
BccI CCATC 5 cut(s) 271, 508, 917, 1005, 1038
BceAI ACGGC 1 cut(s) 902
BciT130I CCWGG 1 cut(s) 751
BciVI GTATCC 1 cut(s) 667
BcnI CCSGG 3 cut(s) 413, 414, 967
BcoDI GTCTC 1 cut(s) 858
BfaI CTAG 5 cut(s) 18, 425, 429, 603, 960
BfmI CTRYAG 3 cut(s) 549, 774, 1104
BfrI CTTAAG 1 cut(s) 347
BfuI GTATCC 1 cut(s) 667
BglII AGATCT 1 cut(s) 431
BisI GCNGC 3 cut(s) 210, 564, 1114
BlsI GCNGC 3 cut(s) 211, 565, 1115
BmcAI AGTACT 2 cut(s) 22, 1080
Bme1390I CCNGG 4 cut(s) 413, 414, 751, 967
Bme18I GGWCC 2 cut(s) 646, 909
BmeRI GACNNNNNGTC 1 cut(s) 907
BmeT110I CYCGRG 1 cut(s) 412
BmgT120I GGNCC 3 cut(s) 35, 646, 909
BmrFI CCNGG 4 cut(s) 413, 414, 751, 967
BmsI GCATC 5 cut(s) 20, 319, 387, 486, 531
BmtI GCTAGC 1 cut(s) 428
BpmI CTGGAG 2 cut(s) 759, 882
BpuEI CTTGAG 1 cut(s) 775
BpuMI CCSGG 3 cut(s) 413, 414, 967
BsaJI CCNNGG 3 cut(s) 412, 413, 966
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 273, 965
Bse1I ACTGG 1 cut(s) 37
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 751
BseDI CCNNGG 3 cut(s) 412, 413, 966
BseGI GGATG 3 cut(s) 11, 282, 1020
BseLI CCNNNNNNNGG 3 cut(s) 97, 273, 965
BseMII CTCAG 3 cut(s) 153, 158, 178
BseNI ACTGG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 1 cut(s) 1125
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 2 cut(s) 557, 972
Bsh1236I CGCG 1 cut(s) 879
BshFI GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
BsiHKCI CYCGRG 1 cut(s) 412
BsiSI CCGG 3 cut(s) 203, 413, 966
BslI CCNNNNNNNGG 3 cut(s) 97, 273, 965
BsmAI GTCTC 1 cut(s) 858
BsmI GAATGC 2 cut(s) 238, 358
BsnI GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
BsoBI CYCGRG 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 165, 1030
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 1 cut(s) 431
BspACI CCGC 5 cut(s) 15, 70, 209, 556, 564
BspANI GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
BspCNI CTCAG 3 cut(s) 152, 157, 177
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 1 cut(s) 879
BspOI GCTAGC 1 cut(s) 428
BspTI CTTAAG 1 cut(s) 347
BsrI ACTGG 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 412, 413, 966
BssMI GATC 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 751
Bst4CI ACNGT 8 cut(s) 111, 535, 550, 685, 820, 872, 988, 1108
BstAFI CTTAAG 1 cut(s) 347
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 3 cut(s) 139, 144, 164
BstF5I GGATG 3 cut(s) 11, 282, 1020
BstFNI CGCG 1 cut(s) 879
BstKTI GATC 1 cut(s) 434
BstMAI GTCTC 1 cut(s) 858
BstMBI GATC 1 cut(s) 431
BstMWI GCNNNNNNNGC 4 cut(s) 23, 218, 227, 895
BstNI CCWGG 1 cut(s) 751
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 4 cut(s) 411, 412, 749, 965
BstSFI CTRYAG 3 cut(s) 549, 774, 1104
BstSLI GKGCMC 1 cut(s) 1030
BstUI CGCG 1 cut(s) 879
BstV1I GCAGC 1 cut(s) 1125
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
BtgZI GCGATG 1 cut(s) 410
BtsCI GGATG 3 cut(s) 11, 282, 1020
BtsIMutI CAGTG 3 cut(s) 172, 936, 1113
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 649
Cfr13I GGNCC 3 cut(s) 35, 646, 909
Cfr9I CCCGGG 1 cut(s) 412
CseI GACGC 1 cut(s) 868
Csp6I GTAC 7 cut(s) 21, 307, 442, 593, 816, 963, 1079
CviAII CATG 6 cut(s) 245, 301, 380, 454, 725, 836
CviQI GTAC 7 cut(s) 21, 307, 442, 593, 816, 963, 1079
DdeI CTNAG 3 cut(s) 139, 144, 164
DpnI GATC 1 cut(s) 433
DpnII GATC 1 cut(s) 431
DraI TTTAAA 1 cut(s) 519
DriI GACNNNNNGTC 1 cut(s) 907
EaeI YGGCCR 2 cut(s) 887, 975
Eam1105I GACNNNNNGTC 1 cut(s) 907
Eco24I GRGCYC 1 cut(s) 165
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 646, 909
Eco57I CTGAAG 1 cut(s) 1059
Eco88I CYCGRG 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 646
EcoRII CCWGG 1 cut(s) 749
EcoRV GATATC 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 165
FaeI CATG 6 cut(s) 248, 304, 383, 457, 728, 839
FatI CATG 6 cut(s) 244, 300, 379, 453, 724, 835
FauI CCCGC 1 cut(s) 549
FauNDI CATATG 1 cut(s) 700
Fnu4HI GCNGC 3 cut(s) 210, 564, 1114
FokI GGATG 2 cut(s) 289, 1027
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 3 cut(s) 210, 564, 1114
FspBI CTAG 5 cut(s) 18, 425, 429, 603, 960
GluI GCNGC 3 cut(s) 210, 564, 1114
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 2 cut(s) 759, 882
HaeIII GGCC 6 cut(s) 36, 90, 221, 260, 889, 977
HapII CCGG 3 cut(s) 203, 413, 966
HgaI GACGC 1 cut(s) 868
Hin1II CATG 6 cut(s) 248, 304, 383, 457, 728, 839
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 5 cut(s) 174, 192, 512, 599, 809
HpaII CCGG 3 cut(s) 203, 413, 966
HphI GGTGA 2 cut(s) 479, 1039
Hpy166II GTNNAC 2 cut(s) 688, 717
Hpy188I TCNGA 2 cut(s) 808, 1006
Hpy188III TCNNGA 7 cut(s) 203, 274, 644, 674, 738, 824, 1123
Hpy8I GTNNAC 2 cut(s) 688, 717
HpyAV CCTTC 2 cut(s) 80, 164
HpyCH4III ACNGT 8 cut(s) 111, 535, 550, 685, 820, 872, 988, 1108
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 7 cut(s) 49, 57, 332, 358, 538, 698, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 218, 227, 895
HpyF3I CTNAG 3 cut(s) 139, 144, 164
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 6 cut(s) 248, 304, 383, 457, 728, 839
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 1 cut(s) 431
LmnI GCTCC 4 cut(s) 523, 740, 770, 1090
Lsp1109I GCAGC 1 cut(s) 1125
LweI GCATC 5 cut(s) 20, 319, 387, 486, 531
MaeI CTAG 5 cut(s) 18, 425, 429, 603, 960
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 3 cut(s) 489, 509, 579
MalI GATC 1 cut(s) 433
MboI GATC 1 cut(s) 431
MboII GAAGA 4 cut(s) 187, 496, 846, 1065
MfeI CAATTG 2 cut(s) 50, 567
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 2 cut(s) 165, 1030
MluCI AATT 3 cut(s) 50, 567, 850
MlyI GAGTC 2 cut(s) 168, 506
MmeI TCCRAC 2 cut(s) 78, 1007
MnlI CCTC 8 cut(s) 66, 199, 211, 250, 745, 799, 1000, 1055
Mph1103I ATGCAT 1 cut(s) 334
MroI TCCGGA 1 cut(s) 202
MseI TTAA 4 cut(s) 335, 348, 518, 710
MslI CAYNNNNRTG 1 cut(s) 243
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 3 cut(s) 203, 413, 966
MspR9I CCNGG 4 cut(s) 413, 414, 751, 967
MunI CAATTG 2 cut(s) 50, 567
Mva1269I GAATGC 2 cut(s) 238, 358
MvaI CCWGG 1 cut(s) 751
MvnI CGCG 1 cut(s) 879
MwoI GCNNNNNNNGC 4 cut(s) 23, 218, 227, 895
NciI CCSGG 3 cut(s) 413, 414, 967
NdeI CATATG 1 cut(s) 700
NdeII GATC 1 cut(s) 431
NheI GCTAGC 1 cut(s) 424
NlaIII CATG 6 cut(s) 248, 304, 383, 457, 728, 839
NmuCI GTSAC 2 cut(s) 509, 579
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 1 cut(s) 248
PciI ACATGT 1 cut(s) 244
PctI GAATGC 2 cut(s) 238, 358
PfeI GAWTC 3 cut(s) 192, 599, 809
PflMI CCANNNNNTGG 1 cut(s) 97
PkrI GCNGC 3 cut(s) 211, 565, 1115
PleI GAGTC 2 cut(s) 168, 506
PpsI GAGTC 2 cut(s) 168, 506
PpuMI RGGWCCY 1 cut(s) 646
PscI ACATGT 1 cut(s) 244
Psp1406I AACGTT 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 1 cut(s) 749
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 1 cut(s) 749
PspPI GGNCC 3 cut(s) 35, 646, 909
PspPPI RGGWCCY 1 cut(s) 646
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 431
RsaI GTAC 7 cut(s) 22, 308, 443, 594, 817, 964, 1080
RsaNI GTAC 7 cut(s) 21, 307, 442, 593, 816, 963, 1079
RseI CAYNNNNRTG 1 cut(s) 243
SaqAI TTAA 4 cut(s) 335, 348, 518, 710
SatI GCNGC 3 cut(s) 210, 564, 1114
Sau3AI GATC 1 cut(s) 431
Sau96I GGNCC 3 cut(s) 35, 646, 909
ScaI AGTACT 2 cut(s) 22, 1080
SchI GAGTC 2 cut(s) 168, 506
ScrFI CCNGG 4 cut(s) 413, 414, 751, 967
SduI GDGCHC 2 cut(s) 165, 1030
SfaNI GCATC 5 cut(s) 20, 319, 387, 486, 531
SfcI CTRYAG 3 cut(s) 549, 774, 1104
SinI GGWCC 2 cut(s) 646, 909
SmaI CCCGGG 1 cut(s) 414
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 3 cut(s) 347, 790, 1123
SmoI CTYRAG 3 cut(s) 347, 790, 1123
Sse9I AATT 3 cut(s) 50, 567, 850
SsiI CCGC 5 cut(s) 15, 70, 209, 556, 564
SspMI CTAG 5 cut(s) 18, 425, 429, 603, 960
StyD4I CCNGG 4 cut(s) 411, 412, 749, 965
TaaI ACNGT 8 cut(s) 111, 535, 550, 685, 820, 872, 988, 1108
TaiI ACGT 1 cut(s) 786
TasI AATT 3 cut(s) 50, 567, 850
TatI WGTACW 3 cut(s) 20, 441, 1078
TauI GCSGC 2 cut(s) 212, 566
TfiI GAWTC 3 cut(s) 192, 599, 809
Tru1I TTAA 4 cut(s) 335, 348, 518, 710
Tru9I TTAA 4 cut(s) 335, 348, 518, 710
TscAI CASTG 3 cut(s) 172, 943, 1113
TseFI GTSAC 2 cut(s) 509, 579
TseI GCWGC 1 cut(s) 1113
Tsp45I GTSAC 2 cut(s) 509, 579
TspDTI ATGAA 1 cut(s) 368
TspGWI ACGGA 1 cut(s) 497
TspMI CCCGGG 1 cut(s) 412
TspRI CASTG 3 cut(s) 172, 943, 1113
Van91I CCANNNNNTGG 1 cut(s) 97
Vha464I CTTAAG 1 cut(s) 347
VpaK11BI GGWCC 2 cut(s) 646, 909
XceI RCATGY 1 cut(s) 248
XmaI CCCGGG 1 cut(s) 412
XspI CTAG 5 cut(s) 18, 425, 429, 603, 960
ZrmI AGTACT 2 cut(s) 22, 1080
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.