MD16G1016000.v1.1

Vacuolar fusion protein CCZ1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005810
Physical Location & Seq
Forward (+)
1227294 .. 1231385
4092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1016000.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGGGCCTGTCGTCGGCCACTACTACGGCTGCCACTCCAGAAGGGGTGCAATTGTGCATATTCGATTTGAGGAGGGGTCAGAACGAAGGGCAGGAGTTGGACAAGATAATCTTCTTCTTTCCAGCTGATTTGCCCTTCTCCGCCCAGTTCTCTGTGATCGGGCTCAGTGAAGGACTCATCACATTTACAAGAATCTTCTCTCCTGAGGCTGCTTGCGACGCCATTGAAGCAGAGAGGCATTCGCATGTCTTTTACGAGGCGGAACCCGATATCTGGATGGTCATGGTAGTGGAGAAAAACAAGGAGTTCGAACCAATTTGGAGAAGTGATGCATTGAGAAAGGTTCTCAAGGAAGTACATTCGCTTTTCGTGATGTTTCATGGATCGATAAGAGCGTTGCTTGATAAGGACCCCGGTGGGGGACTAACTCGTTCCCATCTGTACCATTTCATCATTGACTATCTCGGTGATTTTCTGGTTGGGAAGAAACTGCTGCTTCCCTCATTCCGTGACTCTTTAAAGGAGCGTGGAACTGTACAGATGCTTACCGTAGGGCGGGAAGCGGCAATCGAAGTTCAGTCGCTTGTCAGGGTACTTGAATCTTGTGCTGGGAACATGCCATGTCACTCACTAATCTTGTTTCAGGACCTCTTGGTGTCCACGACACTTTCTCCTGATGATACCGTAAACCTCTTTGCATATGCTGTTTTAAGGCTGAGTCCCCGTGCTTTATCCTCTGGAGTAAGTTCCTGGTCCTATTTACGCAAGGGAGCTACATCGCATGTTTCCTCAAGTTCTAGCTTGGCCCGTCCTGGTTCTATTCCAGAACAATTGCATGGCTCACTTGGCAACTCTCCTGCTGGAGATAAGAGTTCTCAAGTAGTACGGCCCTTGCAGCCTGATAAGTGGTCCAAAGGAAAGGACGGTTTCCTTGTCAGTGATATTTGGGGTGCAGACGCTAGTACTGGAACGTCTGCCACCCCAACAGTTTTTCTCCATCAAACAGAGGAGAGAATGTATCTGTGTGCCCATCAGCATAAGAGCCTTACCTTAATTTTTCTTCTTCCTGTTTCTTCAGTACTAAAAGGTGATCAAGGTGTTTCTGCAGTGAAGCAGCAAGTTCTTGAAAATGTATCCCGAAAGCTGTTGAAAGTTGAAGAGAAATTATCAAAAGGATGGGGTGGTGAGAATGCATATCATGTTAGTGGATATCGGTATTTACTAGCAGATGGAGACAGAAATGTATCCAGGGCTTCTCCACCAGGAAAAGTCACAACCCTTACCAAGGGCTCCTTGCTTGCCTTAAGTAAGGTCAGAGAAGAAGTTGATTTGGAAAAAAGTAGAGCGAAATCGGATAATGCTGGTCACGAGAAAGAATTGGAAATTAGCATCAGGGCGAAAAACAATGCTTGGGTTATCGCTCGGGTTACAAGAGGGAAGGAGCTTTATATGGTTCTAGAGAAAGCCAACGAAACACTTCTTTATGCCTCCGATGCTGTTGAGAAGTTCAGCAACAGGTACTGCAATGGAGCTTTCTCGTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0031982 GO:0043226 GO:0043227 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1712

Protein Analysis

516

Amino Acids

56.73

Weight (kDa)

7.24

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intu_longin_1 PF19031 19 - 128 1.4e-18 First Longin domain of INTU, CCZ1 and HPS4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 141, 260, 556, 563
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 1 cut(s) 15
AcuI CTGAAG 1 cut(s) 1059
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 8 cut(s) 357, 443, 537, 594, 885, 964, 1080, 1520
AfiI CCNNNNNNNGG 6 cut(s) 13, 273, 418, 419, 555, 1285
AflII CTTAAG 1 cut(s) 1303
AgsI TTSAA 6 cut(s) 227, 599, 1127, 1150, 1157, 1543
AjnI CCWGG 4 cut(s) 749, 811, 1247, 1261
AluBI AGCT 6 cut(s) 125, 773, 801, 1144, 1444, 1532
AluI AGCT 6 cut(s) 125, 773, 801, 1144, 1444, 1532
Alw26I GTCTC 1 cut(s) 1227
AlwI GGATC 1 cut(s) 391
AlwNI CAGNNNCTG 1 cut(s) 1521
Ama87I CYCGRG 1 cut(s) 1422
AoxI GGCC 4 cut(s) 4, 15, 804, 887
ApeKI GCWGC 5 cut(s) 29, 209, 493, 895, 1114
Asp700I GAANNNNTTC 1 cut(s) 1476
AspS9I GGNCC 7 cut(s) 4, 409, 646, 753, 805, 888, 909
AsuC2I CCSGG 1 cut(s) 414
AsuHPI GGTGA 3 cut(s) 479, 1100, 1196
AsuII TTCGAA 1 cut(s) 309
AvaI CYCGRG 1 cut(s) 1422
AvaII GGWCC 4 cut(s) 409, 646, 753, 909
AxyI CCTNAGG 1 cut(s) 204
BaeGI GKGCMC 1 cut(s) 1030
BanII GRGCYC 2 cut(s) 165, 1292
BauI CACGAG 1 cut(s) 1367
BbvI GCAGC 5 cut(s) 16, 196, 480, 907, 1126
BccI CCATC 6 cut(s) 271, 444, 1005, 1038, 1170, 1223
BceAI ACGGC 2 cut(s) 42, 902
BciT130I CCWGG 4 cut(s) 751, 813, 1249, 1263
BciVI GTATCC 2 cut(s) 1144, 1255
BclI TGATCA 1 cut(s) 1090
BcnI CCSGG 1 cut(s) 414
BcoDI GTCTC 1 cut(s) 1227
BfaI CTAG 4 cut(s) 798, 960, 1223, 1457
BfmI CTRYAG 1 cut(s) 1104
BfrI CTTAAG 1 cut(s) 1303
BfuI GTATCC 2 cut(s) 1144, 1255
BisI GCNGC 6 cut(s) 30, 210, 494, 564, 896, 1115
BlsI GCNGC 6 cut(s) 31, 211, 495, 565, 897, 1116
BmcAI AGTACT 2 cut(s) 964, 1080
Bme1390I CCNGG 5 cut(s) 414, 751, 813, 1249, 1263
Bme18I GGWCC 4 cut(s) 409, 646, 753, 909
BmeT110I CYCGRG 1 cut(s) 1422
BmgT120I GGNCC 7 cut(s) 4, 409, 646, 753, 805, 888, 909
BmiI GGNNCC 3 cut(s) 264, 411, 1291
BmrFI CCNGG 5 cut(s) 414, 751, 813, 1249, 1263
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 4 cut(s) 319, 531, 1398, 1483
BmuI ACTGGG 1 cut(s) 139
BpmI CTGGAG 3 cut(s) 21, 759, 882
Bpu14I TTCGAA 1 cut(s) 309
BpuEI CTTGAG 3 cut(s) 332, 775, 861
BpuMI CCSGG 1 cut(s) 414
Bsa29I ATCGAT 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 412, 1248, 1284
BsaXI ACNNNNNCTCC 4 cut(s) 655, 685, 1001, 1031
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 273, 418, 419, 555, 1285
Bse1I ACTGG 2 cut(s) 145, 970
Bse21I CCTNAGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 1531
BseBI CCWGG 4 cut(s) 751, 813, 1249, 1263
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 412, 1248, 1284
BseGI GGATG 2 cut(s) 282, 1181
BseLI CCNNNNNNNGG 6 cut(s) 13, 273, 418, 419, 555, 1285
BseMI GCAATG 1 cut(s) 1531
BseMII CTCAG 3 cut(s) 178, 195, 707
BseNI ACTGG 2 cut(s) 145, 970
BseRI GAGGAG 2 cut(s) 85, 1022
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 5 cut(s) 16, 196, 480, 907, 1126
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 972
BshFI GGCC 4 cut(s) 6, 17, 806, 889
BshVI ATCGAT 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 1422
BsiSI CCGG 1 cut(s) 414
BslFI GGGAC 2 cut(s) 435, 705
BslI CCNNNNNNNGG 6 cut(s) 13, 273, 418, 419, 555, 1285
BsmAI GTCTC 1 cut(s) 1227
BsmFI GGGAC 2 cut(s) 435, 705
BsmI GAATGC 2 cut(s) 238, 1195
BsnI GGCC 4 cut(s) 6, 17, 806, 889
BsoBI CYCGRG 1 cut(s) 1422
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 3 cut(s) 165, 1030, 1292
Bsp1407I TGTACA 1 cut(s) 535
Bsp143I GATC 3 cut(s) 156, 383, 1090
BspACI CCGC 4 cut(s) 141, 260, 556, 563
BspANI GGCC 4 cut(s) 6, 17, 806, 889
BspCNI CTCAG 3 cut(s) 177, 196, 708
BspDI ATCGAT 1 cut(s) 386
BspLI GGNNCC 3 cut(s) 264, 411, 1291
BspMAI CTGCAG 1 cut(s) 1108
BspPI GGATC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 309
BspTI CTTAAG 1 cut(s) 1303
BsrDI GCAATG 1 cut(s) 1531
BsrGI TGTACA 1 cut(s) 535
BsrI ACTGG 2 cut(s) 145, 970
BssECI CCNNGG 3 cut(s) 412, 1248, 1284
BssMI GATC 3 cut(s) 156, 383, 1090
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 1367
BssT1I CCWWGG 1 cut(s) 1284
Bst2BI CACGAG 1 cut(s) 1367
Bst2UI CCWGG 4 cut(s) 751, 813, 1249, 1263
Bst4CI ACNGT 5 cut(s) 535, 550, 685, 926, 988
Bst6I CTCTTC 1 cut(s) 1152
BstACI GRCGYC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 1303
BstAUI TGTACA 1 cut(s) 535
BstBI TTCGAA 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 214, 1299
BstDEI CTNAG 3 cut(s) 164, 204, 716
BstENI CCTNNNNNAGG 1 cut(s) 1283
BstF5I GGATG 2 cut(s) 282, 1181
BstKTI GATC 3 cut(s) 159, 386, 1093
BstMAI GTCTC 1 cut(s) 1227
BstMBI GATC 3 cut(s) 156, 383, 1090
BstMWI GCNNNNNNNGC 5 cut(s) 218, 227, 846, 895, 1493
BstNI CCWGG 4 cut(s) 751, 813, 1249, 1263
BstNSI RCATGY 3 cut(s) 248, 619, 785
BstSCI CCNGG 5 cut(s) 412, 749, 811, 1247, 1261
BstSFI CTRYAG 1 cut(s) 1104
BstSLI GKGCMC 1 cut(s) 1030
BstV1I GCAGC 5 cut(s) 16, 196, 480, 907, 1126
Bsu15I ATCGAT 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 204
BsuI GTATCC 2 cut(s) 1144, 1255
BsuRI GGCC 4 cut(s) 6, 17, 806, 889
BsuTUI ATCGAT 1 cut(s) 386
BtgZI GCGATG 1 cut(s) 762
BtsCI GGATG 2 cut(s) 282, 1181
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 3 cut(s) 172, 943, 1113
Cac8I GCNNGC 2 cut(s) 214, 1299
CaiI CAGNNNCTG 1 cut(s) 1521
Cfr13I GGNCC 7 cut(s) 4, 409, 646, 753, 805, 888, 909
ClaI ATCGAT 1 cut(s) 386
CseI GACGC 2 cut(s) 227, 965
Csp6I GTAC 8 cut(s) 356, 442, 536, 593, 884, 963, 1079, 1519
CviAII CATG 8 cut(s) 245, 283, 380, 616, 621, 782, 836, 1199
CviQI GTAC 8 cut(s) 356, 442, 536, 593, 884, 963, 1079, 1519
DdeI CTNAG 3 cut(s) 164, 204, 716
DpnI GATC 3 cut(s) 158, 385, 1092
DpnII GATC 3 cut(s) 156, 383, 1090
DraI TTTAAA 1 cut(s) 519
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 1152
EarI CTCTTC 1 cut(s) 1152
EciI GGCGGA 2 cut(s) 130, 275
Eco130I CCWWGG 1 cut(s) 1284
Eco24I GRGCYC 2 cut(s) 165, 1292
Eco32I GATATC 2 cut(s) 271, 1211
Eco47I GGWCC 4 cut(s) 409, 646, 753, 909
Eco57I CTGAAG 1 cut(s) 1059
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 1422
EcoNI CCTNNNNNAGG 1 cut(s) 1283
EcoO109I RGGNCCY 2 cut(s) 409, 646
EcoRII CCWGG 4 cut(s) 749, 811, 1247, 1261
EcoRV GATATC 2 cut(s) 271, 1211
EcoT14I CCWWGG 1 cut(s) 1284
EcoT22I ATGCAT 2 cut(s) 334, 1195
EcoT38I GRGCYC 2 cut(s) 165, 1292
ErhI CCWWGG 1 cut(s) 1284
FaeI CATG 8 cut(s) 248, 286, 383, 619, 624, 785, 839, 1202
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 1277, 1309
FaqI GGGAC 2 cut(s) 435, 705
FatI CATG 8 cut(s) 244, 282, 379, 615, 620, 781, 835, 1198
FauI CCCGC 1 cut(s) 549
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 1090
Fnu4HI GCNGC 6 cut(s) 30, 210, 494, 564, 896, 1115
FokI GGATG 2 cut(s) 289, 1188
FriOI GRGCYC 2 cut(s) 165, 1292
Fsp4HI GCNGC 6 cut(s) 30, 210, 494, 564, 896, 1115
FspBI CTAG 4 cut(s) 798, 960, 1223, 1457
GluI GCNGC 6 cut(s) 30, 210, 494, 564, 896, 1115
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 3 cut(s) 21, 759, 882
HaeIII GGCC 4 cut(s) 6, 17, 806, 889
HapII CCGG 1 cut(s) 414
HgaI GACGC 2 cut(s) 227, 965
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 8 cut(s) 248, 286, 383, 619, 624, 785, 839, 1202
HinfI GANTC 5 cut(s) 174, 192, 512, 599, 718
HpaII CCGG 1 cut(s) 414
HphI GGTGA 3 cut(s) 479, 1100, 1196
Hpy166II GTNNAC 2 cut(s) 660, 688
Hpy188I TCNGA 4 cut(s) 81, 1316, 1354, 1492
Hpy8I GTNNAC 2 cut(s) 660, 688
Hpy99I CGWCG 2 cut(s) 16, 221
HpyAV CCTTC 5 cut(s) 35, 80, 145, 164, 1432
HpyCH4III ACNGT 5 cut(s) 535, 550, 685, 926, 988
HpyCH4IV ACGT 1 cut(s) 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 227, 846, 895, 1493
HpyF3I CTNAG 3 cut(s) 164, 204, 716
HpySE526I ACGT 1 cut(s) 971
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 8 cut(s) 248, 286, 383, 619, 624, 785, 839, 1202
Ksp22I TGATCA 1 cut(s) 1090
Kzo9I GATC 3 cut(s) 156, 383, 1090
LmnI GCTCC 5 cut(s) 523, 770, 1295, 1441, 1529
Lsp1109I GCAGC 5 cut(s) 16, 196, 480, 907, 1126
LweI GCATC 4 cut(s) 319, 531, 1398, 1483
MaeI CTAG 4 cut(s) 798, 960, 1223, 1457
MaeII ACGT 1 cut(s) 971
MaeIII GTNAC 5 cut(s) 509, 623, 1270, 1364, 1426
MalI GATC 3 cut(s) 158, 385, 1092
MboI GATC 3 cut(s) 156, 383, 1090
MboII GAAGA 9 cut(s) 103, 106, 187, 496, 1052, 1055, 1065, 1169, 1331
MfeI CAATTG 2 cut(s) 50, 830
MhlI GDGCHC 3 cut(s) 165, 1030, 1292
MluCI AATT 8 cut(s) 50, 315, 830, 1053, 1163, 1376, 1383, 1543
MlyI GAGTC 3 cut(s) 168, 506, 727
MmeI TCCRAC 1 cut(s) 78
Mph1103I ATGCAT 2 cut(s) 334, 1195
MroXI GAANNNNTTC 1 cut(s) 1476
MseI TTAA 4 cut(s) 518, 710, 1052, 1304
MslI CAYNNNNRTG 3 cut(s) 243, 287, 1203
MspA1I CMGCKG 1 cut(s) 125
MspCI CTTAAG 1 cut(s) 1303
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 5 cut(s) 414, 751, 813, 1249, 1263
MunI CAATTG 2 cut(s) 50, 830
Mva1269I GAATGC 2 cut(s) 238, 1195
MvaI CCWGG 4 cut(s) 751, 813, 1249, 1263
MwoI GCNNNNNNNGC 5 cut(s) 218, 227, 846, 895, 1493
NciI CCSGG 1 cut(s) 414
NdeI CATATG 1 cut(s) 700
NdeII GATC 3 cut(s) 156, 383, 1090
NlaIII CATG 8 cut(s) 248, 286, 383, 619, 624, 785, 839, 1202
NlaIV GGNNCC 3 cut(s) 264, 411, 1291
NmuCI GTSAC 4 cut(s) 509, 623, 1270, 1364
NsiI ATGCAT 2 cut(s) 334, 1195
NspI RCATGY 3 cut(s) 248, 619, 785
NspV TTCGAA 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PctI GAATGC 2 cut(s) 238, 1195
PdmI GAANNNNTTC 1 cut(s) 1476
PfeI GAWTC 2 cut(s) 192, 599
PkrI GCNGC 6 cut(s) 31, 211, 495, 565, 897, 1116
PleI GAGTC 3 cut(s) 168, 506, 726
PpsI GAGTC 3 cut(s) 168, 506, 726
PpuMI RGGWCCY 2 cut(s) 409, 646
Psp5II RGGWCCY 2 cut(s) 409, 646
Psp6I CCWGG 4 cut(s) 749, 811, 1247, 1261
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 4 cut(s) 749, 811, 1247, 1261
PspN4I GGNNCC 3 cut(s) 264, 411, 1291
PspPI GGNCC 7 cut(s) 4, 409, 646, 753, 805, 888, 909
PspPPI RGGWCCY 2 cut(s) 409, 646
PstI CTGCAG 1 cut(s) 1108
PstNI CAGNNNCTG 1 cut(s) 1521
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 8 cut(s) 357, 443, 537, 594, 885, 964, 1080, 1520
RsaNI GTAC 8 cut(s) 356, 442, 536, 593, 884, 963, 1079, 1519
RseI CAYNNNNRTG 3 cut(s) 243, 287, 1203
SaqAI TTAA 4 cut(s) 518, 710, 1052, 1304
SatI GCNGC 6 cut(s) 30, 210, 494, 564, 896, 1115
Sau3AI GATC 3 cut(s) 156, 383, 1090
Sau96I GGNCC 7 cut(s) 4, 409, 646, 753, 805, 888, 909
ScaI AGTACT 2 cut(s) 964, 1080
SchI GAGTC 3 cut(s) 168, 506, 727
ScrFI CCNGG 5 cut(s) 414, 751, 813, 1249, 1263
SduI GDGCHC 3 cut(s) 165, 1030, 1292
SfaNI GCATC 4 cut(s) 319, 531, 1398, 1483
SfcI CTRYAG 1 cut(s) 1104
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 4 cut(s) 409, 646, 753, 909
SmiMI CAYNNNNRTG 3 cut(s) 243, 287, 1203
SmlI CTYRAG 4 cut(s) 347, 790, 876, 1303
SmoI CTYRAG 4 cut(s) 347, 790, 876, 1303
Sse9I AATT 8 cut(s) 50, 315, 830, 1053, 1163, 1376, 1383, 1543
SsiI CCGC 4 cut(s) 141, 260, 556, 563
SspMI CTAG 4 cut(s) 798, 960, 1223, 1457
StyD4I CCNGG 5 cut(s) 412, 749, 811, 1247, 1261
StyI CCWWGG 1 cut(s) 1284
TaaI ACNGT 5 cut(s) 535, 550, 685, 926, 988
TaiI ACGT 1 cut(s) 974
TaqI TCGA 4 cut(s) 63, 309, 386, 570
TasI AATT 8 cut(s) 50, 315, 830, 1053, 1163, 1376, 1383, 1543
TatI WGTACW 4 cut(s) 355, 535, 962, 1078
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 2 cut(s) 192, 599
Tru1I TTAA 4 cut(s) 518, 710, 1052, 1304
Tru9I TTAA 4 cut(s) 518, 710, 1052, 1304
TscAI CASTG 3 cut(s) 172, 943, 1113
TseFI GTSAC 4 cut(s) 509, 623, 1270, 1364
TseI GCWGC 5 cut(s) 29, 209, 493, 895, 1114
Tsp45I GTSAC 4 cut(s) 509, 623, 1270, 1364
TspDTI ATGAA 2 cut(s) 368, 439
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 3 cut(s) 172, 943, 1113
Vha464I CTTAAG 1 cut(s) 1303
VpaK11BI GGWCC 4 cut(s) 409, 646, 753, 909
XagI CCTNNNNNAGG 1 cut(s) 1283
XbaI TCTAGA 1 cut(s) 1456
XceI RCATGY 3 cut(s) 248, 619, 785
XmnI GAANNNNTTC 1 cut(s) 1476
XspI CTAG 4 cut(s) 798, 960, 1223, 1457
ZrmI AGTACT 2 cut(s) 964, 1080
Zsp2I ATGCAT 2 cut(s) 334, 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.