RLG00000006010

Vacuolar fusion protein CCZ1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005810
Physical Location & Seq
Reverse (-)
3489394 .. 3493291
3898 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006010

Sequence Viewer

Length: 1629 bp
ATGTTTAATCTTCGTTCCAGCCTCCGGCTCAAGGGCATTGCTATGGGTTTGGCATCCGCTAGTACTGCCACTACTGCCCCAGAAGCTATGCAATTGTGCATATTTGATTTGCGGAGGGGTCAAAACGAAGGCCAAGAGTTGGATAAGATACTGTTTTTCTATCCTGCTGATTTGCCTTTCTCAGCTCAGTTCTCTGTGATTGGGCTCAGTGAAGGACTCATCACATTTACCAGAATCTTCTCTCCGGAGGCGGCTTGTGAGGCCATAGAAGCAGATAGGCATTCACATGTTTTTTACGAGGCCGAACCCGATATCTGGATGGTTATGGTAGTGGAGAAAAGCATGGAGTACGAAGCGAAATGGAGAACTGATGCATTAAGAAGGGTTCTTAAGGAAGTGCATTCTCTTTTTGTAATGTTTCATGGTTCTGTAAGAGCGATGCTTGATAAAGACCCGGGGGGAGTGCTAGCTAGATCTCATTTGTACTCTTTCGTCATGGATTATCTTGGTGCATTTGAAAAGCGGTCTCCATTCGATGAGTGCTGCTGGGATTTTCTTGTGGGGAAGAAGCTACTGATGCCATCATTCCGTGACTCTTTAAAGGAGCGTGGAACTGTGCAGATGCTTACTGTAGGGCGGGAAGCCGCAATTGAAGTTCAGTCGCTTGTCAGGGTACTTGAATCTAGTGCTGGGAACTTGGCCTGTCATTCACTAATCTTGTTTCAGGACCTTTTGGTATCCACAACACTTTCTCCTGATGACACCGTAAACCTATTTGCATATGCTGTTTTAAGGTTGACACCCCATGTTTTGTCCTCTGGAGCAAGTTCCTGGTCTTATTTACGCAAAGGAGCTACAGCTAACGTTTCCTCAAGTTCTATCTTGTCCCATTCTGGTGCTGTTCCAGAGCAGTATCATGGCTCACACAACAATTCTTCTGCTGGAGACGACAGTTCTTGCGTCATACGGCCATTGAAGCTGGACAAGTGGTCCAAAGGGAACGATGGTTTTCTTGCCACTGATATTTGGGGTGCGGAGCCTAGTACTGGGGTGTCGGCCACTCCAACAGTATTTCTCCATCAGTCAGAGGAAAGGATGTATCTGTGTGCCCATCAGCATAAAAGCCTCACCTTAATTTTTCTCATTCCTGTTTCTTCAGTACTAAATGGGGAGCAAGGTCTTTCTACAGTGAAGCAGCAAGTTCTTGAGAATGTATCCCTAAAGCTGTTGAAAGTTGAAGAAAAACTATCAAAAGGATGGGGCGGTGAGAACGCATATCATGTTAGTGGATATCGGTATTTACTAGTGGATGGAGACAGAAAGGTATCCAGGGCTTCTCCACCAGGAAAGGTCACAACGCTTAGTAAGGAGTCCTTACTTGCCTTAAGTAAGCTTAGAGAAGAAGTTGATAGAGAAAAGAGTAGAGCACAGTGGGATAGCCCCGGTCATGAAAAAGAGTTGGAAGTTAGCATCAGGGCGAAAAACAATGCTTGGGTTATTGCTCGGGTAACAAGAGGGAAGGAGCTTTACATGGTTCTGGAAAAAGCCAATGAAACACTTCTTTACGCCTCCGATGCTGTTGAGAAGTTCAGCAACAGGTACTGCGGCGGAGCTTTCTCGTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0031982 GO:0043226 GO:0043227 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1712

Protein Analysis

543

Amino Acids

60.13

Weight (kDa)

6.45

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intu_longin_1 PF19031 33 - 142 1.3e-19 First Longin domain of INTU, CCZ1 and HPS4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 139
AccIII TCCGGA 1 cut(s) 244
AclI AACGTT 1 cut(s) 864
AcoI YGGCCR 2 cut(s) 968, 1056
AcuI CTGAAG 1 cut(s) 1140
AfaI GTAC 7 cut(s) 64, 350, 485, 675, 1045, 1161, 1601
AfiI CCNNNNNNNGG 5 cut(s) 24, 31, 139, 315, 1046
AflII CTTAAG 2 cut(s) 389, 1384
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 7 cut(s) 518, 653, 680, 976, 1231, 1238, 1624
AhdI GACNNNNNGTC 1 cut(s) 988
AhlI ACTAGT 1 cut(s) 1303
AjnI CCWGG 3 cut(s) 830, 1328, 1342
Alw21I GWGCWC 1 cut(s) 1429
Alw26I GTCTC 3 cut(s) 531, 939, 1308
AlwNI CAGNNNCTG 1 cut(s) 1602
Ama87I CYCGRG 2 cut(s) 454, 1503
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 6 cut(s) 130, 261, 300, 699, 968, 1056
ApeKI GCWGC 2 cut(s) 543, 1195
Asp700I GAANNNNTTC 1 cut(s) 1557
AspS9I GGNCC 2 cut(s) 727, 990
AsuC2I CCSGG 3 cut(s) 455, 456, 1443
AsuHPI GGTGA 2 cut(s) 1120, 1277
AsuNHI GCTAGC 1 cut(s) 466
AvaI CYCGRG 2 cut(s) 454, 1503
AvaII GGWCC 2 cut(s) 727, 990
BaeGI GKGCMC 1 cut(s) 1111
BanII GRGCYC 1 cut(s) 207
BarI GAAGNNNNNNTAC 2 cut(s) 1511, 1543
Bbv12I GWGCWC 1 cut(s) 1429
BbvI GCAGC 2 cut(s) 530, 1207
BccI CCATC 7 cut(s) 313, 589, 998, 1086, 1119, 1251, 1304
BceAI ACGGC 1 cut(s) 983
BciT130I CCWGG 3 cut(s) 832, 1330, 1344
BciVI GTATCC 3 cut(s) 748, 1225, 1336
BcnI CCSGG 3 cut(s) 455, 456, 1443
BcoDI GTCTC 3 cut(s) 531, 939, 1308
BcuI ACTAGT 1 cut(s) 1303
BfaI CTAG 6 cut(s) 60, 467, 471, 684, 1041, 1304
BfmI CTRYAG 3 cut(s) 630, 855, 1185
BfrI CTTAAG 2 cut(s) 389, 1384
BfuI GTATCC 3 cut(s) 748, 1225, 1336
BglII AGATCT 1 cut(s) 473
BisI GCNGC 5 cut(s) 252, 544, 645, 1196, 1606
BlsI GCNGC 5 cut(s) 253, 545, 646, 1197, 1607
BmcAI AGTACT 3 cut(s) 64, 1045, 1161
Bme1390I CCNGG 6 cut(s) 455, 456, 832, 1330, 1344, 1443
Bme18I GGWCC 2 cut(s) 727, 990
BmeRI GACNNNNNGTC 1 cut(s) 988
BmeT110I CYCGRG 2 cut(s) 454, 1503
BmgT120I GGNCC 2 cut(s) 727, 990
BmiI GGNNCC 1 cut(s) 1038
BmrFI CCNGG 6 cut(s) 455, 456, 832, 1330, 1344, 1443
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 7 cut(s) 62, 361, 429, 567, 612, 1479, 1564
BmtI GCTAGC 1 cut(s) 470
BmuI ACTGGG 1 cut(s) 1056
BpmI CTGGAG 2 cut(s) 840, 963
BpuEI CTTGAG 3 cut(s) 14, 856, 1226
BpuMI CCSGG 3 cut(s) 455, 456, 1443
BsaI GGTCTC 1 cut(s) 531
BsaJI CCNNGG 4 cut(s) 454, 455, 1329, 1441
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 736, 766
Bsc4I CCNNNNNNNGG 5 cut(s) 24, 31, 139, 315, 1046
Bse1I ACTGG 1 cut(s) 1051
Bse3DI GCAATG 1 cut(s) 36
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 3 cut(s) 832, 1330, 1344
BseDI CCNNGG 4 cut(s) 454, 455, 1329, 1441
BseGI GGATG 5 cut(s) 53, 324, 1101, 1262, 1315
BseLI CCNNNNNNNGG 5 cut(s) 24, 31, 139, 315, 1046
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 3 cut(s) 195, 200, 220
BseNI ACTGG 1 cut(s) 1051
BseSI GKGCMC 1 cut(s) 1111
BseXI GCAGC 2 cut(s) 530, 1207
BseYI CCCAGC 2 cut(s) 546, 689
BsgI GTGCAG 1 cut(s) 638
BshFI GGCC 6 cut(s) 132, 263, 302, 701, 970, 1058
BsiHKAI GWGCWC 1 cut(s) 1429
BsiHKCI CYCGRG 2 cut(s) 454, 1503
BsiSI CCGG 4 cut(s) 25, 245, 455, 1443
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 5 cut(s) 24, 31, 139, 315, 1046
BsmAI GTCTC 3 cut(s) 531, 939, 1308
BsmBI CGTCTC 1 cut(s) 939
BsmFI GGGAC 1 cut(s) 871
BsmI GAATGC 2 cut(s) 280, 400
BsnI GGCC 6 cut(s) 132, 263, 302, 701, 970, 1058
Bso31I GGTCTC 1 cut(s) 531
BsoBI CYCGRG 2 cut(s) 454, 1503
Bsp1286I GDGCHC 3 cut(s) 207, 1111, 1429
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 1 cut(s) 473
BspANI GGCC 6 cut(s) 132, 263, 302, 701, 970, 1058
BspCNI CTCAG 3 cut(s) 194, 199, 219
BspEI TCCGGA 1 cut(s) 244
BspHI TCATGA 1 cut(s) 1447
BspLI GGNNCC 1 cut(s) 1038
BspOI GCTAGC 1 cut(s) 470
BspTI CTTAAG 2 cut(s) 389, 1384
BspTNI GGTCTC 1 cut(s) 531
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 1 cut(s) 1051
BssECI CCNNGG 4 cut(s) 454, 455, 1329, 1441
BssMI GATC 1 cut(s) 473
Bst2UI CCWGG 3 cut(s) 832, 1330, 1344
Bst4CI ACNGT 8 cut(s) 153, 616, 631, 766, 953, 1069, 1189, 1431
BstAFI CTTAAG 2 cut(s) 389, 1384
BstC8I GCNNGC 1 cut(s) 468
BstDEI CTNAG 5 cut(s) 181, 186, 206, 1361, 1394
BstF5I GGATG 5 cut(s) 53, 324, 1101, 1262, 1315
BstKTI GATC 1 cut(s) 476
BstMAI GTCTC 3 cut(s) 531, 939, 1308
BstMBI GATC 1 cut(s) 473
BstMWI GCNNNNNNNGC 8 cut(s) 65, 74, 83, 260, 269, 577, 976, 1574
BstNI CCWGG 3 cut(s) 832, 1330, 1344
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 6 cut(s) 453, 454, 830, 1328, 1342, 1441
BstSFI CTRYAG 3 cut(s) 630, 855, 1185
BstSLI GKGCMC 1 cut(s) 1111
BstV1I GCAGC 2 cut(s) 530, 1207
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
BsuI GTATCC 3 cut(s) 748, 1225, 1336
BsuRI GGCC 6 cut(s) 132, 263, 302, 701, 970, 1058
BtgZI GCGATG 1 cut(s) 452
BtsCI GGATG 5 cut(s) 53, 324, 1101, 1262, 1315
BtsIMutI CAGTG 4 cut(s) 214, 1017, 1194, 1436
Cac8I GCNNGC 1 cut(s) 468
CaiI CAGNNNCTG 1 cut(s) 1602
CciI TCATGA 1 cut(s) 1447
Cfr13I GGNCC 2 cut(s) 727, 990
Cfr9I CCCGGG 1 cut(s) 454
CseI GACGC 1 cut(s) 949
Csp6I GTAC 7 cut(s) 63, 349, 484, 674, 1044, 1160, 1600
CviAII CATG 9 cut(s) 287, 343, 422, 496, 806, 917, 1280, 1448, 1531
CviQI GTAC 7 cut(s) 63, 349, 484, 674, 1044, 1160, 1600
DdeI CTNAG 5 cut(s) 181, 186, 206, 1361, 1394
DpnI GATC 1 cut(s) 475
DpnII GATC 1 cut(s) 473
DraI TTTAAA 1 cut(s) 600
DriI GACNNNNNGTC 1 cut(s) 988
EaeI YGGCCR 2 cut(s) 968, 1056
Eam1105I GACNNNNNGTC 1 cut(s) 988
EciI GGCGGA 1 cut(s) 1623
Eco24I GRGCYC 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 531
Eco32I GATATC 2 cut(s) 313, 1292
Eco47I GGWCC 2 cut(s) 727, 990
Eco57I CTGAAG 1 cut(s) 1140
Eco88I CYCGRG 2 cut(s) 454, 1503
EcoO109I RGGNCCY 1 cut(s) 727
EcoRII CCWGG 3 cut(s) 830, 1328, 1342
EcoRV GATATC 2 cut(s) 313, 1292
EcoT22I ATGCAT 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 207
Esp3I CGTCTC 1 cut(s) 939
FaeI CATG 9 cut(s) 290, 346, 425, 499, 809, 920, 1283, 1451, 1534
FalI AAGNNNNNCTT 2 cut(s) 1358, 1390
FaqI GGGAC 1 cut(s) 871
FatI CATG 9 cut(s) 286, 342, 421, 495, 805, 916, 1279, 1447, 1530
FauI CCCGC 1 cut(s) 630
FauNDI CATATG 1 cut(s) 781
Fnu4HI GCNGC 5 cut(s) 252, 544, 645, 1196, 1606
FokI GGATG 5 cut(s) 40, 331, 1108, 1269, 1322
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 5 cut(s) 252, 544, 645, 1196, 1606
FspBI CTAG 6 cut(s) 60, 467, 471, 684, 1041, 1304
GluI GCNGC 5 cut(s) 252, 544, 645, 1196, 1606
GsaI CCCAGC 2 cut(s) 550, 693
GsuI CTGGAG 2 cut(s) 840, 963
HaeIII GGCC 6 cut(s) 132, 263, 302, 701, 970, 1058
HapII CCGG 4 cut(s) 25, 245, 455, 1443
HgaI GACGC 1 cut(s) 949
Hin1II CATG 9 cut(s) 290, 346, 425, 499, 809, 920, 1283, 1451, 1534
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
HindIII AAGCTT 1 cut(s) 1391
HinfI GANTC 5 cut(s) 216, 234, 593, 680, 1370
HpaII CCGG 4 cut(s) 25, 245, 455, 1443
HphI GGTGA 2 cut(s) 1120, 1277
Hpy166II GTNNAC 2 cut(s) 769, 798
Hpy188I TCNGA 2 cut(s) 1087, 1573
Hpy188III TCNNGA 9 cut(s) 245, 316, 725, 755, 819, 905, 1205, 1448, 1538
Hpy8I GTNNAC 2 cut(s) 769, 798
HpyAV CCTTC 4 cut(s) 122, 206, 375, 1513
HpyCH4III ACNGT 8 cut(s) 153, 616, 631, 766, 953, 1069, 1189, 1431
HpyCH4IV ACGT 1 cut(s) 864
HpyCH4V TGCA 7 cut(s) 91, 99, 374, 400, 512, 619, 779
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 74, 83, 260, 269, 577, 976, 1574
HpyF3I CTNAG 5 cut(s) 181, 186, 206, 1361, 1394
HpySE526I ACGT 1 cut(s) 864
Hsp92II CATG 9 cut(s) 290, 346, 425, 499, 809, 920, 1283, 1451, 1534
Kpn2I TCCGGA 1 cut(s) 244
Kzo9I GATC 1 cut(s) 473
LmnI GCTCC 7 cut(s) 604, 821, 851, 1036, 1171, 1522, 1610
Lsp1109I GCAGC 2 cut(s) 530, 1207
LweI GCATC 7 cut(s) 62, 361, 429, 567, 612, 1479, 1564
MaeI CTAG 6 cut(s) 60, 467, 471, 684, 1041, 1304
MaeII ACGT 1 cut(s) 864
MaeIII GTNAC 3 cut(s) 590, 1351, 1507
MalI GATC 1 cut(s) 475
MboI GATC 1 cut(s) 473
MboII GAAGA 6 cut(s) 229, 577, 927, 1146, 1250, 1412
MfeI CAATTG 2 cut(s) 92, 648
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 3 cut(s) 207, 1111, 1429
MluCI AATT 5 cut(s) 92, 648, 931, 1134, 1624
MlyI GAGTC 3 cut(s) 210, 587, 1379
MmeI TCCRAC 3 cut(s) 120, 1088, 1440
Mph1103I ATGCAT 1 cut(s) 376
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 1557
MseI TTAA 7 cut(s) 6, 377, 390, 599, 791, 1133, 1385
MslI CAYNNNNRTG 4 cut(s) 41, 285, 894, 1284
MspCI CTTAAG 2 cut(s) 389, 1384
MspI CCGG 4 cut(s) 25, 245, 455, 1443
MspR9I CCNGG 6 cut(s) 455, 456, 832, 1330, 1344, 1443
MunI CAATTG 2 cut(s) 92, 648
Mva1269I GAATGC 2 cut(s) 280, 400
MvaI CCWGG 3 cut(s) 832, 1330, 1344
MwoI GCNNNNNNNGC 8 cut(s) 65, 74, 83, 260, 269, 577, 976, 1574
NciI CCSGG 3 cut(s) 455, 456, 1443
NdeI CATATG 1 cut(s) 781
NdeII GATC 1 cut(s) 473
NheI GCTAGC 1 cut(s) 466
NlaIII CATG 9 cut(s) 290, 346, 425, 499, 809, 920, 1283, 1451, 1534
NlaIV GGNNCC 1 cut(s) 1038
NmuCI GTSAC 2 cut(s) 590, 1351
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 1 cut(s) 290
PagI TCATGA 1 cut(s) 1447
PciI ACATGT 1 cut(s) 286
PctI GAATGC 2 cut(s) 280, 400
PdmI GAANNNNTTC 1 cut(s) 1557
PfeI GAWTC 2 cut(s) 234, 680
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 5 cut(s) 253, 545, 646, 1197, 1607
PleI GAGTC 3 cut(s) 210, 587, 1378
PpsI GAGTC 3 cut(s) 210, 587, 1378
PpuMI RGGWCCY 1 cut(s) 727
PscI ACATGT 1 cut(s) 286
Psp1406I AACGTT 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 727
Psp6I CCWGG 3 cut(s) 830, 1328, 1342
PspFI CCCAGC 2 cut(s) 546, 689
PspGI CCWGG 3 cut(s) 830, 1328, 1342
PspN4I GGNNCC 1 cut(s) 1038
PspPI GGNCC 2 cut(s) 727, 990
PspPPI RGGWCCY 1 cut(s) 727
PstNI CAGNNNCTG 1 cut(s) 1602
PsuI RGATCY 1 cut(s) 473
RsaI GTAC 7 cut(s) 64, 350, 485, 675, 1045, 1161, 1601
RsaNI GTAC 7 cut(s) 63, 349, 484, 674, 1044, 1160, 1600
RseI CAYNNNNRTG 4 cut(s) 41, 285, 894, 1284
SaqAI TTAA 7 cut(s) 6, 377, 390, 599, 791, 1133, 1385
SatI GCNGC 5 cut(s) 252, 544, 645, 1196, 1606
Sau3AI GATC 1 cut(s) 473
Sau96I GGNCC 2 cut(s) 727, 990
ScaI AGTACT 3 cut(s) 64, 1045, 1161
SchI GAGTC 3 cut(s) 210, 587, 1379
ScrFI CCNGG 6 cut(s) 455, 456, 832, 1330, 1344, 1443
SduI GDGCHC 3 cut(s) 207, 1111, 1429
SfaNI GCATC 7 cut(s) 62, 361, 429, 567, 612, 1479, 1564
SfcI CTRYAG 3 cut(s) 630, 855, 1185
SinI GGWCC 2 cut(s) 727, 990
SmaI CCCGGG 1 cut(s) 456
SmiMI CAYNNNNRTG 4 cut(s) 41, 285, 894, 1284
SmlI CTYRAG 5 cut(s) 29, 389, 871, 1205, 1384
SmoI CTYRAG 5 cut(s) 29, 389, 871, 1205, 1384
SpeI ACTAGT 1 cut(s) 1303
Sse9I AATT 5 cut(s) 92, 648, 931, 1134, 1624
SspMI CTAG 6 cut(s) 60, 467, 471, 684, 1041, 1304
StyD4I CCNGG 6 cut(s) 453, 454, 830, 1328, 1342, 1441
TaaI ACNGT 8 cut(s) 153, 616, 631, 766, 953, 1069, 1189, 1431
TaiI ACGT 1 cut(s) 867
TaqI TCGA 1 cut(s) 534
TasI AATT 5 cut(s) 92, 648, 931, 1134, 1624
TatI WGTACW 4 cut(s) 62, 483, 1043, 1159
TauI GCSGC 3 cut(s) 254, 647, 1608
TfiI GAWTC 2 cut(s) 234, 680
Tru1I TTAA 7 cut(s) 6, 377, 390, 599, 791, 1133, 1385
Tru9I TTAA 7 cut(s) 6, 377, 390, 599, 791, 1133, 1385
TscAI CASTG 4 cut(s) 214, 1024, 1194, 1436
TseFI GTSAC 2 cut(s) 590, 1351
TseI GCWGC 2 cut(s) 543, 1195
Tsp45I GTSAC 2 cut(s) 590, 1351
TspDTI ATGAA 3 cut(s) 410, 1464, 1566
TspGWI ACGGA 1 cut(s) 578
TspMI CCCGGG 1 cut(s) 454
TspRI CASTG 4 cut(s) 214, 1024, 1194, 1436
Van91I CCANNNNNTGG 1 cut(s) 139
Vha464I CTTAAG 2 cut(s) 389, 1384
VpaK11BI GGWCC 2 cut(s) 727, 990
XceI RCATGY 1 cut(s) 290
XmaI CCCGGG 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 1557
XspI CTAG 6 cut(s) 60, 467, 471, 684, 1041, 1304
ZrmI AGTACT 3 cut(s) 64, 1045, 1161
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.