MD13G1022300.v1.1

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014102
Physical Location & Seq
Reverse (-)
1607451 .. 1607797
347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1022300.v1.1.491

Sequence Viewer

Length: 282 bp
ATGTTGCTGTACAAGCAGAAGAAGAACCAGAGCACCGCCAAGGGCAACAGGCTCTTGATCAGCATCACTGTGCTCGGCAGTGCCGGCCCGATCCGCTTTGTTGTCAATGAGCAGGAGGTCATCGCCGCGGTCATGGACACCGCCTTGAAATCCTATGCGCGTGAGGGCCGCTTCCCGGTTCTCGGCTCCGATCTCAATGACTTCCTGCTCTACTGCCCCGCCGTAGGACCTGAAGGTAACCAATCCTTAATCACTTTTGAATTCACCAATCTAATCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

94

Amino Acids

10.21

Weight (kDa)

7.86

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 15 - 76 6.2e-20 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23150 AT1G70780
fragaria_vesca FvH4_4g32830
malus_domestica MD13G1022300.v1.1 MD16G1023400.v1.1
prunus_persica Prupe.1G329500_v2.0.a1
pyrus_communis pycom13g02080
rosa_chinensis RchiOBHm_Chr4g0441841
rosa_laevigata RLG00000006061
rosa_multiflora Rmu_sc0008316.1_g000006
rosa_roxburghii Rroxscaffold_5G00382670
rosa_rugosa Rorug04G0336600
rosa_samantha Rh4AG388000 Rh4BG401700 Rh4CG416700 Rh4DG395100
rosa_wichuraiana Rw4G033500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 128, 160
AciI CCGC 7 cut(s) 36, 94, 126, 128, 141, 169, 219
AclWI GGATC 1 cut(s) 85
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 252
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 3 cut(s) 175, 182, 224
AgsI TTSAA 2 cut(s) 148, 260
Alw21I GWGCWC 2 cut(s) 35, 75
AlwI GGATC 1 cut(s) 85
AoxI GGCC 2 cut(s) 85, 166
ApoI RAATTY 1 cut(s) 260
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 3 cut(s) 86, 166, 227
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 256
AvaII GGWCC 1 cut(s) 227
Bbv12I GWGCWC 2 cut(s) 35, 75
BceAI ACGGC 1 cut(s) 206
BclI TGATCA 1 cut(s) 57
BcnI CCSGG 1 cut(s) 176
BisI GCNGC 2 cut(s) 126, 169
BlsI GCNGC 2 cut(s) 127, 170
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 3 cut(s) 86, 166, 227
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 39, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 182, 224
Bse118I RCCGGY 1 cut(s) 83
Bse8I GATNNNNATC 1 cut(s) 62
BseDI CCNNGG 2 cut(s) 39, 126
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 3 cut(s) 175, 182, 224
Bsh1236I CGCG 2 cut(s) 128, 160
BshFI GGCC 2 cut(s) 87, 168
BsiHKAI GWGCWC 2 cut(s) 35, 75
BsiSI CCGG 2 cut(s) 84, 176
BslI CCNNNNNNNGG 3 cut(s) 175, 182, 224
BsnI GGCC 2 cut(s) 87, 168
Bsp1286I GDGCHC 2 cut(s) 35, 75
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 3 cut(s) 57, 90, 190
BspACI CCGC 7 cut(s) 36, 94, 126, 128, 141, 169, 219
BspANI GGCC 2 cut(s) 87, 168
BspFNI CGCG 2 cut(s) 128, 160
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 83
BsrGI TGTACA 1 cut(s) 9
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 39, 126
BssMI GATC 3 cut(s) 57, 90, 190
BssT1I CCWWGG 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 70
BstAUI TGTACA 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 85
BstDSI CCRYGG 1 cut(s) 126
BstEII GGTNACC 1 cut(s) 236
BstFNI CGCG 2 cut(s) 128, 160
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 60, 93, 193
BstMBI GATC 3 cut(s) 57, 90, 190
BstMWI GCNNNNNNNGC 3 cut(s) 13, 84, 93
BstPI GGTNACC 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 174
BstUI CGCG 2 cut(s) 128, 160
BsuRI GGCC 2 cut(s) 87, 168
BtgI CCRYGG 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 2 cut(s) 66, 85
Cac8I GCNNGC 1 cut(s) 85
CfoI GCGC 1 cut(s) 160
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 3 cut(s) 86, 166, 227
Cfr42I CCGCGG 1 cut(s) 129
Csp6I GTAC 1 cut(s) 10
CviAII CATG 1 cut(s) 133
CviJI RGCY 4 cut(s) 52, 87, 168, 186
CviKI_1 RGCY 4 cut(s) 52, 87, 168, 186
CviQI GTAC 1 cut(s) 10
DpnI GATC 3 cut(s) 59, 92, 192
DpnII GATC 3 cut(s) 57, 90, 190
Eco130I CCWWGG 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 252
Eco91I GGTNACC 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 1 cut(s) 136
FaiI YATR 2 cut(s) 134, 156
FatI CATG 1 cut(s) 132
FauI CCCGC 1 cut(s) 226
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 2 cut(s) 126, 169
Fsp4HI GCNGC 2 cut(s) 126, 169
GlaI GCGC 1 cut(s) 159
GluI GCNGC 2 cut(s) 126, 169
HaeIII GGCC 2 cut(s) 87, 168
HapII CCGG 2 cut(s) 84, 176
HhaI GCGC 1 cut(s) 160
Hin1II CATG 1 cut(s) 136
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HpaII CCGG 2 cut(s) 84, 176
HphI GGTGA 1 cut(s) 256
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 1 cut(s) 55
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 84, 93
Hsp92II CATG 1 cut(s) 136
HspAI GCGC 1 cut(s) 158
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 57
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 3 cut(s) 57, 90, 190
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 7 cut(s) 34, 41, 97, 98, 189, 218, 243
LweI GCATC 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 236
MalI GATC 3 cut(s) 59, 92, 192
MboI GATC 3 cut(s) 57, 90, 190
MboII GAAGA 2 cut(s) 31, 34
MhlI GDGCHC 2 cut(s) 35, 75
MluCI AATT 1 cut(s) 260
MnlI CCTC 2 cut(s) 109, 157
MroNI GCCGGC 1 cut(s) 83
MseI TTAA 2 cut(s) 248, 280
MslI CAYNNNNRTG 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 2 cut(s) 84, 176
MspR9I CCNGG 1 cut(s) 176
MvnI CGCG 2 cut(s) 128, 160
MwoI GCNNNNNNNGC 3 cut(s) 13, 84, 93
NaeI GCCGGC 1 cut(s) 85
NciI CCSGG 1 cut(s) 176
NdeII GATC 3 cut(s) 57, 90, 190
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 1 cut(s) 187
NmeAIII GCCGAG 2 cut(s) 54, 162
PdiI GCCGGC 1 cut(s) 85
PkrI GCNGC 2 cut(s) 127, 170
PpuMI RGGWCCY 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 227
PspEI GGTNACC 1 cut(s) 236
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 3 cut(s) 86, 166, 227
PspPPI RGGWCCY 1 cut(s) 227
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 68
SacII CCGCGG 1 cut(s) 129
SaqAI TTAA 2 cut(s) 248, 280
SatI GCNGC 2 cut(s) 126, 169
Sau3AI GATC 3 cut(s) 57, 90, 190
Sau96I GGNCC 3 cut(s) 86, 166, 227
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 2 cut(s) 35, 75
SetI ASST 3 cut(s) 120, 232, 238
SfaNI GCATC 1 cut(s) 72
Sfr303I CCGCGG 1 cut(s) 129
SgrBI CCGCGG 1 cut(s) 129
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 68
Sse9I AATT 1 cut(s) 260
SsiI CCGC 7 cut(s) 36, 94, 126, 128, 141, 169, 219
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 70
TasI AATT 1 cut(s) 260
TatI WGTACW 1 cut(s) 9
TauI GCSGC 2 cut(s) 128, 171
Tru1I TTAA 2 cut(s) 248, 280
Tru9I TTAA 2 cut(s) 248, 280
TscAI CASTG 2 cut(s) 73, 85
TspRI CASTG 2 cut(s) 73, 85
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.