Rorug04G0336600

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014102
Physical Location & Seq
Forward (+)
51047636 .. 51048346
711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0336600.1

Sequence Viewer

Length: 684 bp
ATGGAACCACAAAACCGGCATCCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTGTAGGCGGAGCAGGTGGACTCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTATAGAGCAGGTTCAGAGGATCTATCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACGCTCGGGAGAAGCAGAAGAAGAAGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGAAAATGTTGCCAATTCATATGCACCTGCTTCTGCTTCTTCTGCTGGTGTGATGATTGCTTTTAACGGGAAGATGGGGAACTATGGCGGTTATGGATCCATGAACATGGAGAAGAGTGCTAGGGATTGTTCAATCTTAGCTGGAGGGGGAACTAGTTCTACTTTCTTTGACATGAATGTAGAACAAACTTTCATGGAACAAATAGGAGAAGATCACCAGGAAATTGAAACCCTTTCACTGTTTCCCGTGCATGGTGAGGACATCTTTGGCAACATGAAGACTACTTCCGATGGAGGTGGCGGTCACGGTGGTGGCTCTCAAACTTCCCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTGGAAAAAGTTTCCGGAGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

24.75

Weight (kDa)

8.37

Isoelectric Point (pI)

57.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23150 AT1G70780
fragaria_vesca FvH4_4g32830
malus_domestica MD13G1022300.v1.1 MD16G1023400.v1.1
prunus_persica Prupe.1G329500_v2.0.a1
pyrus_communis pycom13g02080
rosa_chinensis RchiOBHm_Chr4g0441841
rosa_laevigata RLG00000006061
rosa_multiflora Rmu_sc0008316.1_g000006
rosa_roxburghii Rroxscaffold_5G00382670
rosa_rugosa Rorug04G0336600
rosa_samantha Rh4AG388000 Rh4BG401700 Rh4CG416700 Rh4DG395100
rosa_wichuraiana Rw4G033500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 78, 334
Acc36I ACCTGC 3 cut(s) 78, 160, 334
AccIII TCCGGA 1 cut(s) 672
AciI CCGC 4 cut(s) 82, 387, 600, 679
AclWI GGATC 3 cut(s) 188, 390, 403
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 551
AgsI TTSAA 3 cut(s) 220, 432, 527
AhlI ACTAGT 1 cut(s) 452
AjnI CCWGG 1 cut(s) 516
AleI CACNNNNGTG 1 cut(s) 609
AluBI AGCT 5 cut(s) 130, 193, 440, 634, 659
AluI AGCT 5 cut(s) 130, 193, 440, 634, 659
Alw21I GWGCWC 1 cut(s) 636
AlwI GGATC 3 cut(s) 188, 390, 403
Ama87I CYCGRG 1 cut(s) 228
Aor13HI TCCGGA 1 cut(s) 672
ApeKI GCWGC 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 308, 506, 566
AvaI CYCGRG 1 cut(s) 228
AvaII GGWCC 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 273
BamHI GGATCC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 636
BbsI GAAGAC 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 1 cut(s) 62
BccI CCATC 3 cut(s) 26, 367, 584
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 518
BcnI CCSGG 1 cut(s) 55
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 2 cut(s) 420, 453
BfmI CTRYAG 2 cut(s) 102, 162
BfuAI ACCTGC 3 cut(s) 78, 160, 334
BisI GCNGC 2 cut(s) 51, 679
BlpI GCTNAGC 1 cut(s) 635
BlsI GCNGC 2 cut(s) 52, 680
Bme1390I CCNGG 2 cut(s) 55, 518
Bme18I GGWCC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 4 cut(s) 6, 155, 397, 677
BmrFI CCNGG 2 cut(s) 55, 518
BmsI GCATC 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 584
BpmI CTGGAG 2 cut(s) 173, 462
Bpu1102I GCTNAGC 1 cut(s) 635
BpuEI CTTGAG 2 cut(s) 107, 650
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 672
Bsc4I CCNNNNNNNGG 1 cut(s) 551
Bse118I RCCGGY 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 672
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 3 cut(s) 19, 37, 265
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 551
BseMII CTCAG 1 cut(s) 649
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 636
BsiHKCI CYCGRG 1 cut(s) 228
BsiSI CCGG 3 cut(s) 16, 54, 673
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 551
BsmFI GGGAC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 228
Bsp1286I GDGCHC 2 cut(s) 273, 636
Bsp13I TCCGGA 1 cut(s) 672
Bsp143I GATC 6 cut(s) 106, 180, 210, 281, 395, 511
Bsp1720I GCTNAGC 1 cut(s) 635
BspACI CCGC 4 cut(s) 82, 387, 600, 679
BspCNI CTCAG 1 cut(s) 648
BspEI TCCGGA 1 cut(s) 672
BspLI GGNNCC 4 cut(s) 6, 155, 397, 677
BspMI ACCTGC 3 cut(s) 78, 160, 334
BspPI GGATC 3 cut(s) 188, 390, 403
BsrFI RCCGGY 1 cut(s) 15
BssAI RCCGGY 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 6 cut(s) 106, 180, 210, 281, 395, 511
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 2 cut(s) 540, 608
Bst6I CTCTTC 1 cut(s) 407
BstDEI CTNAG 2 cut(s) 436, 635
BstF5I GGATG 3 cut(s) 19, 37, 265
BstKTI GATC 6 cut(s) 109, 183, 213, 284, 398, 514
BstMBI GATC 6 cut(s) 106, 180, 210, 281, 395, 511
BstMWI GCNNNNNNNGC 3 cut(s) 79, 341, 645
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 53, 516
BstSFI CTRYAG 2 cut(s) 102, 162
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 2 cut(s) 180, 395
BstXI CCANNNNNNTGG 1 cut(s) 406
BstYI RGATCY 2 cut(s) 180, 395
BtsCI GGATG 3 cut(s) 19, 37, 265
BtsIMutI CAGTG 1 cut(s) 536
BveI ACCTGC 3 cut(s) 78, 160, 334
Cfr10I RCCGGY 1 cut(s) 15
Cfr13I GGNCC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 202
CviAII CATG 9 cut(s) 70, 266, 274, 400, 406, 472, 493, 551, 574
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 436, 635
DpnI GATC 6 cut(s) 108, 182, 212, 283, 397, 513
DpnII GATC 6 cut(s) 106, 180, 210, 281, 395, 511
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 634
Eco24I GRGCYC 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 634
Eco88I CYCGRG 1 cut(s) 228
EcoICRI GAGCTC 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 636
FaeI CATG 9 cut(s) 73, 269, 277, 403, 409, 475, 496, 554, 577
FaqI GGGAC 1 cut(s) 139
FatI CATG 9 cut(s) 69, 265, 273, 399, 405, 471, 492, 550, 573
FauNDI CATATG 1 cut(s) 319
Fnu4HI GCNGC 2 cut(s) 51, 679
FokI GGATG 3 cut(s) 6, 44, 272
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 2 cut(s) 51, 679
FspBI CTAG 2 cut(s) 420, 453
GluI GCNGC 2 cut(s) 51, 679
GsuI CTGGAG 2 cut(s) 173, 462
HapII CCGG 3 cut(s) 16, 54, 673
Hin1II CATG 9 cut(s) 73, 269, 277, 403, 409, 475, 496, 554, 577
HinfI GANTC 2 cut(s) 94, 117
HpaII CCGG 3 cut(s) 16, 54, 673
HphI GGTGA 3 cut(s) 308, 506, 566
Hpy166II GTNNAC 2 cut(s) 93, 252
Hpy188I TCNGA 4 cut(s) 111, 177, 259, 589
Hpy188III TCNNGA 3 cut(s) 220, 230, 673
Hpy8I GTNNAC 2 cut(s) 93, 252
HpyCH4III ACNGT 2 cut(s) 540, 608
HpyCH4V TGCA 3 cut(s) 277, 323, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 341, 645
HpyF3I CTNAG 2 cut(s) 436, 635
Hsp92II CATG 9 cut(s) 73, 269, 277, 403, 409, 475, 496, 554, 577
Kpn2I TCCGGA 1 cut(s) 672
Kzo9I GATC 6 cut(s) 106, 180, 210, 281, 395, 511
LmnI GCTCC 3 cut(s) 84, 127, 675
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 28
MaeI CTAG 2 cut(s) 420, 453
MaeIII GTNAC 1 cut(s) 602
MalI GATC 6 cut(s) 108, 182, 212, 283, 397, 513
MboI GATC 6 cut(s) 106, 180, 210, 281, 395, 511
MboII GAAGA 7 cut(s) 253, 256, 330, 382, 424, 521, 589
MflI RGATCY 2 cut(s) 180, 395
MhlI GDGCHC 2 cut(s) 273, 636
MluCI AATT 2 cut(s) 313, 522
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 8 cut(s) 22, 29, 131, 171, 437, 550, 587, 643
MroI TCCGGA 1 cut(s) 672
MroXI GAANNNNTTC 1 cut(s) 454
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 2 cut(s) 404, 609
MspA1I CMGCKG 2 cut(s) 193, 681
MspI CCGG 3 cut(s) 16, 54, 673
MspR9I CCNGG 2 cut(s) 55, 518
MvaI CCWGG 1 cut(s) 518
MwoI GCNNNNNNNGC 3 cut(s) 79, 341, 645
NciI CCSGG 1 cut(s) 55
NdeI CATATG 1 cut(s) 319
NdeII GATC 6 cut(s) 106, 180, 210, 281, 395, 511
NlaIII CATG 9 cut(s) 73, 269, 277, 403, 409, 475, 496, 554, 577
NlaIV GGNNCC 4 cut(s) 6, 155, 397, 677
NmuCI GTSAC 1 cut(s) 602
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 609
PaqCI CACCTGC 2 cut(s) 78, 334
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 117
PkrI GCNGC 2 cut(s) 52, 680
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 636
Psp5II RGGWCCY 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 4 cut(s) 6, 155, 397, 677
PspPI GGNCC 1 cut(s) 153
PspPPI RGGWCCY 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 442, 474
PsuI RGATCY 2 cut(s) 180, 395
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 2 cut(s) 404, 609
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 2 cut(s) 51, 679
Sau3AI GATC 6 cut(s) 106, 180, 210, 281, 395, 511
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 55, 518
SduI GDGCHC 2 cut(s) 273, 636
SfaNI GCATC 1 cut(s) 28
SfcI CTRYAG 2 cut(s) 102, 162
SinI GGWCC 1 cut(s) 153
SmiMI CAYNNNNRTG 2 cut(s) 404, 609
SmlI CTYRAG 2 cut(s) 122, 629
SmoI CTYRAG 2 cut(s) 122, 629
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 2 cut(s) 313, 522
SsiI CCGC 4 cut(s) 82, 387, 600, 679
SspMI CTAG 2 cut(s) 420, 453
SstI GAGCTC 1 cut(s) 636
StyD4I CCNGG 2 cut(s) 53, 516
TaaI ACNGT 2 cut(s) 540, 608
TaqI TCGA 1 cut(s) 213
TasI AATT 2 cut(s) 313, 522
TauI GCSGC 1 cut(s) 681
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 602
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 7 cut(s) 254, 306, 315, 416, 481, 488, 590
TspRI CASTG 1 cut(s) 543
VpaK11BI GGWCC 1 cut(s) 153
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 454
XspI CTAG 2 cut(s) 420, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.