Rw4G033500

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014102
Physical Location & Seq
Forward (+)
60672655 .. 60673220
566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033500.1

Sequence Viewer

Length: 408 bp
ATGTTGCTGTACAAGCAGAAGAAGAGTCCCAAGGGCAACAGGTTCCTGATCAGCATCACTGTGCTGGGCAGTGCCGGCCCGATCCGATTCGTTGTCAACGAGGAGGCGCTTGTCGCTGCGGTCATCGACACCGCCTTGAAATCCTATGCTCGTGAAGGTCGCCTTCCCATTCTCGGCTCCGATCTCAATGAGTTCATGCTCTACTGCCCCACTGCTGGACCTGATGCTCTCAGTCCATGGGACACAATTGGATCGCAAGGAGCTCGGAACTTTATGCTATGCAAGAAGCCTCAGCCGGTGAAAATGGCCGACAGTGAAATACCGGCAGCTGCAATTTCTCGCAAGGGGAGTGGGAGCTGGAAGGCATGGATCAACAAGTCCCTCAACCTCAAAATCTCTTCCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.66

Weight (kDa)

9.74

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 12 - 94 1.6e-29 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23150 AT1G70780
fragaria_vesca FvH4_4g32830
malus_domestica MD13G1022300.v1.1 MD16G1023400.v1.1
prunus_persica Prupe.1G329500_v2.0.a1
pyrus_communis pycom13g02080
rosa_chinensis RchiOBHm_Chr4g0441841
rosa_laevigata RLG00000006061
rosa_multiflora Rmu_sc0008316.1_g000006
rosa_roxburghii Rroxscaffold_5G00382670
rosa_rugosa Rorug04G0336600
rosa_samantha Rh4AG388000 Rh4BG401700 Rh4CG416700 Rh4DG395100
rosa_wichuraiana Rw4G033500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 119, 132
AclWI GGATC 3 cut(s) 76, 259, 377
AcoI YGGCCR 1 cut(s) 306
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 173, 215
AgsI TTSAA 1 cut(s) 139
AluBI AGCT 3 cut(s) 263, 329, 357
AluI AGCT 3 cut(s) 263, 329, 357
Alw21I GWGCWC 1 cut(s) 265
AlwI GGATC 3 cut(s) 76, 259, 377
AoxI GGCC 2 cut(s) 76, 306
ApeKI GCWGC 3 cut(s) 116, 326, 329
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 2 cut(s) 77, 218
AsuHPI GGTGA 1 cut(s) 310
AvaII GGWCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 265
BauI CACGAG 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 265
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 3 cut(s) 103, 316, 338
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BclI TGATCA 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 110
BisI GCNGC 3 cut(s) 117, 327, 330
BlsI GCNGC 3 cut(s) 118, 328, 331
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 2 cut(s) 77, 218
BmiI GGNNCC 2 cut(s) 44, 178
BmsI GCATC 2 cut(s) 63, 214
Bpu10I CCTNAGC 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 30, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 215
Bse118I RCCGGY 3 cut(s) 74, 295, 322
Bse8I GATNNNNATC 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 30, 236
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 2 cut(s) 173, 215
BseMII CTCAG 2 cut(s) 244, 305
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 3 cut(s) 103, 316, 338
BseYI CCCAGC 1 cut(s) 64
BshFI GGCC 2 cut(s) 78, 308
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 3 cut(s) 75, 296, 323
BslFI GGGAC 3 cut(s) 12, 254, 364
BslI CCNNNNNNNGG 2 cut(s) 173, 215
BsmFI GGGAC 3 cut(s) 12, 254, 364
BsnI GGCC 2 cut(s) 78, 308
Bsp1286I GDGCHC 1 cut(s) 265
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 5 cut(s) 48, 81, 181, 251, 369
Bsp19I CCATGG 1 cut(s) 236
BspACI CCGC 2 cut(s) 119, 132
BspANI GGCC 2 cut(s) 78, 308
BspCNI CTCAG 2 cut(s) 243, 304
BspLI GGNNCC 2 cut(s) 44, 178
BspPI GGATC 3 cut(s) 76, 259, 377
BsrFI RCCGGY 3 cut(s) 74, 295, 322
BsrGI TGTACA 1 cut(s) 9
BssAI RCCGGY 3 cut(s) 74, 295, 322
BssECI CCNNGG 2 cut(s) 30, 236
BssMI GATC 5 cut(s) 48, 81, 181, 251, 369
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 2 cut(s) 30, 236
Bst2BI CACGAG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 61, 314
Bst6I CTCTTC 2 cut(s) 17, 403
BstAUI TGTACA 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 2 cut(s) 230, 291
BstDSI CCRYGG 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 5 cut(s) 51, 84, 184, 254, 372
BstMBI GATC 5 cut(s) 48, 81, 181, 251, 369
BstMWI GCNNNNNNNGC 3 cut(s) 13, 75, 113
BstV1I GCAGC 3 cut(s) 103, 316, 338
BsuRI GGCC 2 cut(s) 78, 308
BtgI CCRYGG 1 cut(s) 236
BtsI GCAGTG 2 cut(s) 76, 210
BtsIMutI CAGTG 4 cut(s) 57, 76, 210, 319
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 3 cut(s) 74, 295, 322
Cfr13I GGNCC 2 cut(s) 77, 218
Csp6I GTAC 1 cut(s) 10
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 3 cut(s) 196, 237, 366
CviJI RGCY 8 cut(s) 78, 177, 263, 289, 295, 308, 329, 357
CviKI_1 RGCY 8 cut(s) 78, 177, 263, 289, 295, 308, 329, 357
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 2 cut(s) 230, 291
DpnI GATC 5 cut(s) 50, 83, 183, 253, 371
DpnII GATC 5 cut(s) 48, 81, 181, 251, 369
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 2 cut(s) 17, 403
EarI CTCTTC 2 cut(s) 17, 403
Ecl136II GAGCTC 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 30, 236
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 218
Eco53kI GAGCTC 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 263
EcoT14I CCWWGG 2 cut(s) 30, 236
EcoT38I GRGCYC 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 30, 236
FaeI CATG 3 cut(s) 199, 240, 369
FaiI YATR 6 cut(s) 147, 197, 238, 275, 280, 367
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FaqI GGGAC 3 cut(s) 12, 254, 364
FatI CATG 3 cut(s) 195, 236, 365
FbaI TGATCA 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 117, 327, 330
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 3 cut(s) 117, 327, 330
GlaI GCGC 1 cut(s) 108
GluI GCNGC 3 cut(s) 117, 327, 330
GsaI CCCAGC 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 2 cut(s) 78, 308
HapII CCGG 3 cut(s) 75, 296, 323
HhaI GCGC 1 cut(s) 109
Hin1II CATG 3 cut(s) 199, 240, 369
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 2 cut(s) 25, 87
HpaII CCGG 3 cut(s) 75, 296, 323
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 3 cut(s) 86, 181, 267
Hpy188III TCNNGA 2 cut(s) 46, 152
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 3 cut(s) 149, 173, 355
HpyCH4III ACNGT 2 cut(s) 61, 314
HpyCH4V TGCA 2 cut(s) 282, 332
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 75, 113
HpyF3I CTNAG 2 cut(s) 230, 291
Hsp92II CATG 3 cut(s) 199, 240, 369
HspAI GCGC 1 cut(s) 107
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 5 cut(s) 48, 81, 181, 251, 369
LmnI GCTCC 3 cut(s) 182, 260, 354
LpnPI CCDG 9 cut(s) 25, 50, 59, 88, 201, 234, 309, 336, 343
Lsp1109I GCAGC 3 cut(s) 103, 316, 338
LweI GCATC 2 cut(s) 63, 214
MalI GATC 5 cut(s) 50, 83, 183, 253, 371
MboI GATC 5 cut(s) 48, 81, 181, 251, 369
MboII GAAGA 3 cut(s) 31, 34, 390
MfeI CAATTG 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 2 cut(s) 246, 333
MlyI GAGTC 1 cut(s) 34
MnlI CCTC 5 cut(s) 94, 97, 300, 392, 398
MroNI GCCGGC 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 3 cut(s) 75, 296, 323
MunI CAATTG 1 cut(s) 246
MwoI GCNNNNNNNGC 3 cut(s) 13, 75, 113
NaeI GCCGGC 1 cut(s) 76
NcoI CCATGG 1 cut(s) 236
NdeII GATC 5 cut(s) 48, 81, 181, 251, 369
NgoMIV GCCGGC 1 cut(s) 74
NlaIII CATG 3 cut(s) 199, 240, 369
NlaIV GGNNCC 2 cut(s) 44, 178
NmeAIII GCCGAG 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 76
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 3 cut(s) 118, 328, 331
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 64
PspN4I GGNNCC 2 cut(s) 44, 178
PspPI GGNCC 2 cut(s) 77, 218
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 265
SatI GCNGC 3 cut(s) 117, 327, 330
Sau3AI GATC 5 cut(s) 48, 81, 181, 251, 369
Sau96I GGNCC 2 cut(s) 77, 218
SchI GAGTC 1 cut(s) 34
SduI GDGCHC 1 cut(s) 265
SetI ASST 7 cut(s) 44, 160, 223, 265, 331, 359, 390
SfaNI GCATC 2 cut(s) 63, 214
SinI GGWCC 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 59
Sse9I AATT 2 cut(s) 246, 333
SsiI CCGC 2 cut(s) 119, 132
SstI GAGCTC 1 cut(s) 265
StyI CCWWGG 2 cut(s) 30, 236
TaaI ACNGT 2 cut(s) 61, 314
TaqI TCGA 1 cut(s) 126
TasI AATT 2 cut(s) 246, 333
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 1 cut(s) 87
TscAI CASTG 4 cut(s) 64, 76, 217, 319
TseI GCWGC 3 cut(s) 116, 326, 329
TspDTI ATGAA 1 cut(s) 184
TspRI CASTG 4 cut(s) 64, 76, 217, 319
VpaK11BI GGWCC 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.