MD16G1023400.v1.1

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014102
Physical Location & Seq
Reverse (-)
1709332 .. 1711273
1942 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1023400.v1.1.491

Sequence Viewer

Length: 417 bp
ATGTTGCTGTACAAGCAGAAGAAGAACCAGAGCACCGCCAAGGGCAACAGGCTCTTGATCAGCATCACTGTGCTCGGCAGTGCTGGCCCGATCCGCTTCGTTGTCAACGAGCAGGAGCTCGTCGCCGCCGTCATGGACACCGCTTTGAAATCCTATGCGCGTGAGGGGCGCCTCCCGGTTCTTGGCTCCGATCTCAATGACTTCCTGCTCTACTGCCCCGCCGTAGGACCTGAAGCTCTGAGCCCGTGGGACACAATCGGGGCGCAAGGAGCTCGTAACTTTATGCTGTGCAAGAAGCCTCAGCCAGTGAAGATGGAGAAGGATGAGATACCAGCAGCTGCAATTATTCGCAAGGGTAGTGGGAGCTGGAAGGCATGGATCAACAAGTCCCTCAATCTCAAGGTCTCTTCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.08

Weight (kDa)

9.7

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 15 - 97 7.3e-29 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23150 AT1G70780
fragaria_vesca FvH4_4g32830
malus_domestica MD13G1022300.v1.1 MD16G1023400.v1.1
prunus_persica Prupe.1G329500_v2.0.a1
pyrus_communis pycom13g02080
rosa_chinensis RchiOBHm_Chr4g0441841
rosa_laevigata RLG00000006061
rosa_multiflora Rmu_sc0008316.1_g000006
rosa_roxburghii Rroxscaffold_5G00382670
rosa_rugosa Rorug04G0336600
rosa_samantha Rh4AG388000 Rh4BG401700 Rh4CG416700 Rh4DG395100
rosa_wichuraiana Rw4G033500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 168
AccII CGCG 1 cut(s) 160
AciI CCGC 5 cut(s) 36, 94, 126, 141, 219
AclWI GGATC 2 cut(s) 85, 386
AcuI CTGAAG 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 169
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 182, 224
AgsI TTSAA 1 cut(s) 148
AluBI AGCT 5 cut(s) 118, 236, 272, 338, 366
AluI AGCT 5 cut(s) 118, 236, 272, 338, 366
Alw21I GWGCWC 4 cut(s) 35, 75, 120, 274
Alw26I GTCTC 1 cut(s) 409
AlwI GGATC 2 cut(s) 85, 386
AlwNI CAGNNNCTG 1 cut(s) 338
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 2 cut(s) 335, 338
AspLEI GCGC 3 cut(s) 160, 171, 265
AspS9I GGNCC 2 cut(s) 86, 227
AsuC2I CCSGG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 227
BanI GGYRCC 1 cut(s) 168
BanII GRGCYC 3 cut(s) 120, 245, 274
Bbv12I GWGCWC 4 cut(s) 35, 75, 120, 274
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 325, 347
BccI CCATC 1 cut(s) 307
BceAI ACGGC 2 cut(s) 113, 206
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BclI TGATCA 1 cut(s) 57
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 1 cut(s) 409
BfoI RGCGCY 1 cut(s) 172
BisI GCNGC 3 cut(s) 126, 336, 339
BlsI GCNGC 3 cut(s) 127, 337, 340
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 86, 227
BmiI GGNNCC 2 cut(s) 170, 187
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 1 cut(s) 72
Bpu10I CCTNAGC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 62
BsaHI GRCGYC 1 cut(s) 169
BsaI GGTCTC 1 cut(s) 409
BsaJI CCNNGG 2 cut(s) 39, 245
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 224
Bse1I ACTGG 1 cut(s) 305
Bse8I GATNNNNATC 1 cut(s) 62
BseDI CCNNGG 2 cut(s) 39, 245
BseGI GGATG 1 cut(s) 328
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 2 cut(s) 182, 224
BseMII CTCAG 2 cut(s) 230, 314
BseNI ACTGG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 325, 347
Bsh1236I CGCG 1 cut(s) 160
BshFI GGCC 1 cut(s) 87
BshNI GGYRCC 1 cut(s) 168
BsiHKAI GWGCWC 4 cut(s) 35, 75, 120, 274
BsiSI CCGG 1 cut(s) 176
BslFI GGGAC 2 cut(s) 263, 373
BslI CCNNNNNNNGG 2 cut(s) 182, 224
BsmAI GTCTC 1 cut(s) 409
BsmFI GGGAC 2 cut(s) 263, 373
BsnI GGCC 1 cut(s) 87
Bso31I GGTCTC 1 cut(s) 409
Bsp1286I GDGCHC 5 cut(s) 35, 75, 120, 245, 274
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 4 cut(s) 57, 90, 190, 378
BspACI CCGC 5 cut(s) 36, 94, 126, 141, 219
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 2 cut(s) 231, 313
BspFNI CGCG 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 170, 187
BspPI GGATC 2 cut(s) 85, 386
BspT107I GGYRCC 1 cut(s) 168
BspTNI GGTCTC 1 cut(s) 409
BsrGI TGTACA 1 cut(s) 9
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 2 cut(s) 39, 245
BssMI GATC 4 cut(s) 57, 90, 190, 378
BssNI GRCGYC 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 412
BstACI GRCGYC 1 cut(s) 169
BstAUI TGTACA 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 239, 300
BstDSI CCRYGG 1 cut(s) 245
BstF5I GGATG 1 cut(s) 328
BstFNI CGCG 1 cut(s) 160
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 3 cut(s) 160, 171, 265
BstKTI GATC 4 cut(s) 60, 93, 193, 381
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 4 cut(s) 57, 90, 190, 378
BstMWI GCNNNNNNNGC 5 cut(s) 13, 84, 93, 166, 269
BstSCI CCNGG 1 cut(s) 174
BstUI CGCG 1 cut(s) 160
BstV1I GCAGC 2 cut(s) 325, 347
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 245
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 3 cut(s) 66, 85, 312
Cac8I GCNNGC 1 cut(s) 85
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 3 cut(s) 160, 171, 265
Cfr13I GGNCC 2 cut(s) 86, 227
Csp6I GTAC 1 cut(s) 10
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 2 cut(s) 133, 375
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 2 cut(s) 239, 300
DinI GGCGCC 1 cut(s) 170
DpnI GATC 4 cut(s) 59, 92, 192, 380
DpnII GATC 4 cut(s) 57, 90, 190, 378
Eam1104I CTCTTC 1 cut(s) 412
EarI CTCTTC 1 cut(s) 412
Ecl136II GAGCTC 2 cut(s) 118, 272
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 3 cut(s) 120, 245, 274
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 227
Eco53kI GAGCTC 2 cut(s) 118, 272
Eco57I CTGAAG 1 cut(s) 252
EcoICRI GAGCTC 2 cut(s) 118, 272
EcoO109I RGGNCCY 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 3 cut(s) 120, 245, 274
EgeI GGCGCC 1 cut(s) 170
EheI GGCGCC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 2 cut(s) 136, 378
FaiI YATR 4 cut(s) 134, 156, 284, 376
FaqI GGGAC 2 cut(s) 263, 373
FatI CATG 2 cut(s) 132, 374
FauI CCCGC 1 cut(s) 226
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 3 cut(s) 126, 336, 339
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 3 cut(s) 120, 245, 274
Fsp4HI GCNGC 3 cut(s) 126, 336, 339
GlaI GCGC 3 cut(s) 159, 170, 264
GluI GCNGC 3 cut(s) 126, 336, 339
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 1 cut(s) 87
HapII CCGG 1 cut(s) 176
HhaI GCGC 3 cut(s) 160, 171, 265
Hin1I GRCGYC 1 cut(s) 169
Hin1II CATG 2 cut(s) 136, 378
Hin6I GCGC 3 cut(s) 158, 169, 263
HinP1I GCGC 3 cut(s) 158, 169, 263
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HpaII CCGG 1 cut(s) 176
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 190, 240
Hpy188III TCNNGA 1 cut(s) 55
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 2 cut(s) 313, 364
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 291, 341
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 84, 93, 166, 269
HpyF3I CTNAG 2 cut(s) 239, 300
Hsp92I GRCGYC 1 cut(s) 169
Hsp92II CATG 2 cut(s) 136, 378
HspAI GCGC 3 cut(s) 158, 169, 263
KasI GGCGCC 1 cut(s) 168
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 4 cut(s) 57, 90, 190, 378
LmnI GCTCC 4 cut(s) 115, 191, 269, 363
Lsp1109I GCAGC 2 cut(s) 325, 347
LweI GCATC 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 275
MalI GATC 4 cut(s) 59, 92, 192, 380
MboI GATC 4 cut(s) 57, 90, 190, 378
MboII GAAGA 4 cut(s) 31, 34, 322, 399
MhlI GDGCHC 5 cut(s) 35, 75, 120, 245, 274
MluCI AATT 1 cut(s) 342
Mly113I GGCGCC 1 cut(s) 169
MnlI CCTC 4 cut(s) 157, 182, 309, 401
MslI CAYNNNNRTG 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 1 cut(s) 176
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 5 cut(s) 13, 84, 93, 166, 269
NarI GGCGCC 1 cut(s) 169
NciI CCSGG 1 cut(s) 176
NdeII GATC 4 cut(s) 57, 90, 190, 378
NlaIII CATG 2 cut(s) 136, 378
NlaIV GGNNCC 2 cut(s) 170, 187
NmeAIII GCCGAG 1 cut(s) 54
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 126
PkrI GCNGC 3 cut(s) 127, 337, 340
PluTI GGCGCC 1 cut(s) 172
PpuMI RGGWCCY 1 cut(s) 227
Psp124BI GAGCTC 2 cut(s) 120, 274
Psp5II RGGWCCY 1 cut(s) 227
PspN4I GGNNCC 2 cut(s) 170, 187
PspPI GGNCC 2 cut(s) 86, 227
PspPPI RGGWCCY 1 cut(s) 227
PstNI CAGNNNCTG 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 68
SacI GAGCTC 2 cut(s) 120, 274
SatI GCNGC 3 cut(s) 126, 336, 339
Sau3AI GATC 4 cut(s) 57, 90, 190, 378
Sau96I GGNCC 2 cut(s) 86, 227
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 5 cut(s) 35, 75, 120, 245, 274
SetI ASST 7 cut(s) 120, 232, 238, 274, 340, 368, 405
SfaNI GCATC 1 cut(s) 72
SfoI GGCGCC 1 cut(s) 170
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 1 cut(s) 342
SsiI CCGC 5 cut(s) 36, 94, 126, 141, 219
SspDI GGCGCC 1 cut(s) 168
SstI GAGCTC 2 cut(s) 120, 274
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 70
TasI AATT 1 cut(s) 342
TatI WGTACW 1 cut(s) 9
TauI GCSGC 1 cut(s) 128
TscAI CASTG 3 cut(s) 73, 85, 312
TseI GCWGC 2 cut(s) 335, 338
TspRI CASTG 3 cut(s) 73, 85, 312
VpaK11BI GGWCC 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.