MD13G1027100.v1.1

Xylosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016227
Physical Location & Seq
Reverse (-)
1960031 .. 1962887
2857 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1027100.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGGGAGCTGAGAAGAAATGGCTCCTTACGCTCTTCTCAGCCACATTCCTCTCCCTCCTCCTCCTCCTCCTCTCCTCCATCTCCGCCTTCAGCTCACCCAAACCCTTCCCTTCAATTGTCCAGCACGGCTCTAGCTACCCGCCCGCTTTCGCTTACTACATATGGGGCGGCCGAGGCGATAGGGCTCGGATCTTCCGCCTGCTGCTCGCCGTCTACCATCCCCGGAATCGATATTTGTTGCATCTTTCGGCTGACGAATCTGAAGACGAGCGCCGCCGTCTTGCCGCCGCTATCAACGCTGTGCCGGCAATTCAGGCATTTGGGAATGTGGATGTGGTGGGCAAGCCTGATCGAATCACTTACATGGGGTCTTCCAATATTGCCACCACTTTGCACGCGGCGGCGATTTTTCTTAAGGTTGATAGTGGGTGGGATTGGTTCGTCACTTTGAGCGCCATGGATTATCCACTTATCACTCAGGACGACCTGTCGCATGTTTTCTCCTCTGTTAGAAGAGATCTCAATTTCATTGATCACACCAGCGACCTTGGATGGAAAGAGTTGCACAGGGTCCAGCCAATTGTAGTTGATCCGGGGATATACCTAGCTCGAAGGAGCCAAATTTTTCATGCTACAGAGAAGCGGAAGACCCCTGATGCTTTTAAAGTATTCACAGGCTCCCCATGGGTCATCCTGAGCAGATCATTTCTGGAGTTTTGCATTAATGGTTGGGATAACCTTCCCCGGACCCTGCTTATGTACTTTACAAATGTGATGTTGTCCCAGGAAGGATATTTCCATTCAGTTATATGCAATTCACCAGAGTTCAAGAACACTACTGTGAACAGTGATCTAAGATACGTGATCTGGGACACTCCCCCGAAAATGGAACCCCTTTTTCTCAACACCTTGGATTTTGATCAAATGGCTCAAAGTGGAGCTGCTTTTGCCCGACAATTCAAGAAAGATGACCGTGTGCTGGACATGGTTGATAAAAAGATCCTCAAGCGTGGGAGAAATCGAGCTGCCCCGGGGGCATGGTGCTCAGGCTGGAGAAGTTGGTGGATGGATCCTTGCACTCAATGGAGTGATGCCAATATCTTGAAGCCTGGGCCCCAAGCAAAGAAGCTCGAAGACTCGATTACTAACCTTCTCGATGACTGGAATGCGCAAACAAATCAATGCCAATGA

Protein Analysis

397

Amino Acids

45.17

Weight (kDa)

8.69

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 50 - 309 7.2e-65 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016227)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71070
fragaria_vesca FvH4_4g32220
malus_domestica MD13G1027100.v1.1 MD16G1030200.v1.1
prunus_persica Prupe.1G322800_v2.0.a1
pyrus_communis pycom13g02580 pycom16g02570
rosa_chinensis RchiOBHm_Chr4g0440961
rosa_laevigata RLG00000006136
rosa_multiflora Rmu_sc0000424.1_g000027
rosa_roxburghii Rroxscaffold_5G00381820
rosa_rugosa Rorug04G0329700
rosa_samantha Rh4AG381500 Rh4DG388000
rosa_wichuraiana Rw4G032860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1170
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 398
AclWI GGATC 5 cut(s) 197, 584, 994, 1064, 1077
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 621
AcuI CTGAAG 2 cut(s) 73, 282
AfaI GTAC 1 cut(s) 761
AfiI CCNNNNNNNGG 2 cut(s) 886, 979
AflII CTTAAG 1 cut(s) 413
AgsI TTSAA 4 cut(s) 114, 829, 961, 1105
AhdI GACNNNNNGTC 1 cut(s) 487
AjnI CCWGG 2 cut(s) 783, 1108
AleI CACNNNNGTG 1 cut(s) 839
AluBI AGCT 7 cut(s) 8, 93, 135, 608, 941, 1025, 1129
AluI AGCT 7 cut(s) 8, 93, 135, 608, 941, 1025, 1129
Alw21I GWGCWC 1 cut(s) 1046
AlwI GGATC 5 cut(s) 197, 584, 994, 1064, 1077
Ama87I CYCGRG 1 cut(s) 1030
AoxI GGCC 2 cut(s) 169, 1112
ApaI GGGCCC 1 cut(s) 1116
ApeKI GCWGC 3 cut(s) 202, 941, 1025
ApoI RAATTY 1 cut(s) 621
AseI ATTAAT 1 cut(s) 723
AspLEI GCGC 3 cut(s) 273, 455, 1171
AspS9I GGNCC 4 cut(s) 571, 747, 1112, 1113
AsuC2I CCSGG 5 cut(s) 223, 594, 745, 1031, 1032
AsuHPI GGTGA 2 cut(s) 87, 810
AvaI CYCGRG 1 cut(s) 1030
AvaII GGWCC 2 cut(s) 571, 747
BaeGI GKGCMC 1 cut(s) 1116
BamHI GGATCC 1 cut(s) 1069
BanII GRGCYC 2 cut(s) 187, 1116
BbsI GAAGAC 4 cut(s) 270, 363, 653, 1140
Bbv12I GWGCWC 1 cut(s) 1046
BbvI GCAGC 3 cut(s) 189, 928, 1012
BccI CCATC 4 cut(s) 86, 225, 546, 1060
BceAI ACGGC 3 cut(s) 142, 194, 261
BciT130I CCWGG 2 cut(s) 785, 1110
BclI TGATCA 2 cut(s) 532, 919
BcnI CCSGG 5 cut(s) 223, 594, 745, 1031, 1032
BfaI CTAG 2 cut(s) 132, 605
BfmI CTRYAG 1 cut(s) 633
BfoI RGCGCY 2 cut(s) 274, 456
BfrI CTTAAG 1 cut(s) 413
BglI GCCNNNNNGGC 1 cut(s) 1034
BglII AGATCT 1 cut(s) 517
BisI GCNGC 9 cut(s) 169, 203, 274, 285, 288, 399, 402, 942, 1026
BlsI GCNGC 9 cut(s) 170, 204, 275, 286, 289, 400, 403, 943, 1027
Bme1390I CCNGG 7 cut(s) 223, 594, 745, 785, 1031, 1032, 1110
Bme18I GGWCC 2 cut(s) 571, 747
BmeRI GACNNNNNGTC 1 cut(s) 487
BmeT110I CYCGRG 1 cut(s) 1030
BmgT120I GGNCC 4 cut(s) 571, 747, 1112, 1113
BmiI GGNNCC 9 cut(s) 23, 572, 617, 679, 749, 891, 1071, 1114, 1115
BmrFI CCNGG 7 cut(s) 223, 594, 745, 785, 1031, 1032, 1110
BmsI GCATC 3 cut(s) 250, 646, 1081
BpiI GAAGAC 4 cut(s) 270, 363, 653, 1140
BpmI CTGGAG 2 cut(s) 731, 1072
Bpu10I CCTNAGC 2 cut(s) 695, 1045
BpuEI CTTGAG 1 cut(s) 989
BpuMI CCSGG 5 cut(s) 223, 594, 745, 1031, 1032
Bsa29I ATCGAT 1 cut(s) 229
BsaAI YACGTR 1 cut(s) 862
BsaBI GATNNNNATC 1 cut(s) 918
Bsc4I CCNNNNNNNGG 2 cut(s) 886, 979
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 1 cut(s) 1166
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 2 cut(s) 785, 1110
BseCI ATCGAT 1 cut(s) 229
BseGI GGATG 5 cut(s) 217, 337, 557, 690, 1071
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 2 cut(s) 886, 979
BseMII CTCAG 4 cut(s) 51, 491, 686, 1059
BseNI ACTGG 1 cut(s) 1166
BseRI GAGGAG 7 cut(s) 47, 50, 53, 56, 59, 64, 493
BseSI GKGCMC 1 cut(s) 1116
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 3 cut(s) 189, 928, 1012
Bsh1236I CGCG 1 cut(s) 398
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 2 cut(s) 171, 1114
BshVI ATCGAT 1 cut(s) 229
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 1046
BsiHKCI CYCGRG 1 cut(s) 1030
BsiSI CCGG 5 cut(s) 223, 305, 593, 745, 1031
BslFI GGGAC 2 cut(s) 766, 884
BslI CCNNNNNNNGG 2 cut(s) 886, 979
BsmFI GGGAC 2 cut(s) 766, 884
BsmI GAATGC 1 cut(s) 1171
BsnI GGCC 2 cut(s) 171, 1114
BsoBI CYCGRG 1 cut(s) 1030
Bsp120I GGGCCC 1 cut(s) 1112
Bsp1286I GDGCHC 3 cut(s) 187, 1046, 1116
Bsp19I CCATGG 2 cut(s) 456, 683
BspANI GGCC 2 cut(s) 171, 1114
BspCNI CTCAG 4 cut(s) 50, 490, 687, 1058
BspDI ATCGAT 1 cut(s) 229
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 9 cut(s) 23, 572, 617, 679, 749, 891, 1071, 1114, 1115
BspPI GGATC 5 cut(s) 197, 584, 994, 1064, 1077
BspQI GCTCTTC 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 1 cut(s) 1166
BssAI RCCGGY 1 cut(s) 304
BssT1I CCWWGG 4 cut(s) 456, 547, 683, 909
Bst2UI CCWGG 2 cut(s) 785, 1110
Bst4CI ACNGT 3 cut(s) 841, 848, 974
Bst6I CTCTTC 2 cut(s) 38, 508
BstAFI CTTAAG 1 cut(s) 413
BstBAI YACGTR 1 cut(s) 862
BstC8I GCNNGC 6 cut(s) 144, 200, 207, 306, 344, 396
BstDEI CTNAG 6 cut(s) 9, 37, 477, 695, 854, 1045
BstDSI CCRYGG 2 cut(s) 456, 683
BstF5I GGATG 5 cut(s) 217, 337, 557, 690, 1071
BstFNI CGCG 1 cut(s) 398
BstH2I RGCGCY 2 cut(s) 274, 456
BstHHI GCGC 3 cut(s) 273, 455, 1171
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 7 cut(s) 28, 174, 296, 305, 314, 947, 1034
BstNI CCWGG 2 cut(s) 785, 1110
BstNSI RCATGY 1 cut(s) 497
BstSCI CCNGG 7 cut(s) 221, 592, 743, 783, 1029, 1030, 1108
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 1 cut(s) 1116
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 3 cut(s) 189, 928, 1012
BstV2I GAAGAC 4 cut(s) 270, 363, 653, 1140
BstX2I RGATCY 4 cut(s) 189, 517, 999, 1069
BstYI RGATCY 4 cut(s) 189, 517, 999, 1069
BstZI CGGCCG 1 cut(s) 169
Bsu15I ATCGAT 1 cut(s) 229
BsuRI GGCC 2 cut(s) 171, 1114
BsuTUI ATCGAT 1 cut(s) 229
BtgI CCRYGG 2 cut(s) 456, 683
BtsCI GGATG 5 cut(s) 217, 337, 557, 690, 1071
BtsIMutI CAGTG 1 cut(s) 853
Cac8I GCNNGC 6 cut(s) 144, 200, 207, 306, 344, 396
CfoI GCGC 3 cut(s) 273, 455, 1171
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 4 cut(s) 571, 747, 1112, 1113
Cfr9I CCCGGG 1 cut(s) 1030
ClaI ATCGAT 1 cut(s) 229
Csp6I GTAC 1 cut(s) 760
CviAII CATG 7 cut(s) 364, 457, 494, 629, 684, 985, 1038
CviQI GTAC 1 cut(s) 760
DdeI CTNAG 6 cut(s) 9, 37, 477, 695, 854, 1045
DraI TTTAAA 1 cut(s) 664
DriI GACNNNNNGTC 1 cut(s) 487
EaeI YGGCCR 1 cut(s) 169
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 2 cut(s) 38, 508
Eam1105I GACNNNNNGTC 1 cut(s) 487
EarI CTCTTC 2 cut(s) 38, 508
EciI GGCGGA 2 cut(s) 73, 185
EclXI CGGCCG 1 cut(s) 169
Eco130I CCWWGG 4 cut(s) 456, 547, 683, 909
Eco24I GRGCYC 2 cut(s) 187, 1116
Eco47I GGWCC 2 cut(s) 571, 747
Eco52I CGGCCG 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 73, 282
Eco88I CYCGRG 1 cut(s) 1030
EcoO109I RGGNCCY 1 cut(s) 1113
EcoRII CCWGG 2 cut(s) 783, 1108
EcoT14I CCWWGG 4 cut(s) 456, 547, 683, 909
EcoT38I GRGCYC 2 cut(s) 187, 1116
ErhI CCWWGG 4 cut(s) 456, 547, 683, 909
FaeI CATG 7 cut(s) 367, 460, 497, 632, 687, 988, 1041
FaqI GGGAC 2 cut(s) 766, 884
FatI CATG 7 cut(s) 363, 456, 493, 628, 683, 984, 1037
FauI CCCGC 2 cut(s) 147, 151
FauNDI CATATG 1 cut(s) 161
FbaI TGATCA 2 cut(s) 532, 919
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 9 cut(s) 169, 203, 274, 285, 288, 399, 402, 942, 1026
FokI GGATG 5 cut(s) 204, 344, 564, 677, 1078
FriOI GRGCYC 2 cut(s) 187, 1116
Fsp4HI GCNGC 9 cut(s) 169, 203, 274, 285, 288, 399, 402, 942, 1026
FspBI CTAG 2 cut(s) 132, 605
FspI TGCGCA 1 cut(s) 1170
GlaI GCGC 3 cut(s) 272, 454, 1170
GluI GCNGC 9 cut(s) 169, 203, 274, 285, 288, 399, 402, 942, 1026
GsuI CTGGAG 2 cut(s) 731, 1072
HaeII RGCGCY 2 cut(s) 274, 456
HaeIII GGCC 2 cut(s) 171, 1114
HapII CCGG 5 cut(s) 223, 305, 593, 745, 1031
HhaI GCGC 3 cut(s) 273, 455, 1171
Hin1II CATG 7 cut(s) 367, 460, 497, 632, 687, 988, 1041
Hin6I GCGC 3 cut(s) 271, 453, 1169
HinP1I GCGC 3 cut(s) 271, 453, 1169
HinfI GANTC 4 cut(s) 226, 257, 354, 1136
HpaII CCGG 5 cut(s) 223, 305, 593, 745, 1031
HphI GGTGA 2 cut(s) 87, 810
Hpy166II GTNNAC 2 cut(s) 214, 844
Hpy188I TCNGA 2 cut(s) 189, 262
Hpy188III TCNNGA 7 cut(s) 479, 694, 710, 829, 961, 1102, 1154
Hpy8I GTNNAC 2 cut(s) 214, 844
HpyAV CCTTC 7 cut(s) 97, 115, 120, 606, 749, 782, 1160
HpyCH4III ACNGT 3 cut(s) 841, 848, 974
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 6 cut(s) 241, 394, 565, 720, 813, 1077
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 174, 296, 305, 314, 947, 1034
HpyF3I CTNAG 6 cut(s) 9, 37, 477, 695, 854, 1045
HpySE526I ACGT 1 cut(s) 861
Hsp92II CATG 7 cut(s) 367, 460, 497, 632, 687, 988, 1041
HspAI GCGC 3 cut(s) 271, 453, 1169
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Ksp22I TGATCA 2 cut(s) 532, 919
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 5 cut(s) 5, 27, 615, 683, 938
Lsp1109I GCAGC 3 cut(s) 189, 928, 1012
LweI GCATC 3 cut(s) 250, 646, 1081
MaeI CTAG 2 cut(s) 132, 605
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 1 cut(s) 442
MboII GAAGA 8 cut(s) 25, 25, 184, 275, 363, 525, 658, 1145
MfeI CAATTG 2 cut(s) 114, 579
MflI RGATCY 4 cut(s) 189, 517, 999, 1069
MhlI GDGCHC 3 cut(s) 187, 1046, 1116
MluCI AATT 7 cut(s) 114, 309, 523, 579, 621, 814, 956
MlyI GAGTC 1 cut(s) 1130
MroNI GCCGGC 1 cut(s) 304
MseI TTAA 3 cut(s) 414, 663, 723
MslI CAYNNNNRTG 2 cut(s) 362, 839
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 5 cut(s) 223, 305, 593, 745, 1031
MspR9I CCNGG 7 cut(s) 223, 594, 745, 785, 1031, 1032, 1110
MunI CAATTG 2 cut(s) 114, 579
Mva1269I GAATGC 1 cut(s) 1171
MvaI CCWGG 2 cut(s) 785, 1110
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 7 cut(s) 28, 174, 296, 305, 314, 947, 1034
NaeI GCCGGC 1 cut(s) 306
NciI CCSGG 5 cut(s) 223, 594, 745, 1031, 1032
NcoI CCATGG 2 cut(s) 456, 683
NdeI CATATG 1 cut(s) 161
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 7 cut(s) 367, 460, 497, 632, 687, 988, 1041
NlaIV GGNNCC 9 cut(s) 23, 572, 617, 679, 749, 891, 1071, 1114, 1115
NmeAIII GCCGAG 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 442
NsbI TGCGCA 1 cut(s) 1170
NspI RCATGY 1 cut(s) 497
OliI CACNNNNGTG 1 cut(s) 839
PciSI GCTCTTC 1 cut(s) 38
PctI GAATGC 1 cut(s) 1171
PdiI GCCGGC 1 cut(s) 306
PfeI GAWTC 3 cut(s) 226, 257, 354
PkrI GCNGC 9 cut(s) 170, 204, 275, 286, 289, 400, 403, 943, 1027
PleI GAGTC 1 cut(s) 1130
PpsI GAGTC 1 cut(s) 1130
Ppu21I YACGTR 1 cut(s) 862
PshBI ATTAAT 1 cut(s) 723
Psp6I CCWGG 2 cut(s) 783, 1108
PspGI CCWGG 2 cut(s) 783, 1108
PspN4I GGNNCC 9 cut(s) 23, 572, 617, 679, 749, 891, 1071, 1114, 1115
PspOMI GGGCCC 1 cut(s) 1112
PspPI GGNCC 4 cut(s) 571, 747, 1112, 1113
PsuI RGATCY 4 cut(s) 189, 517, 999, 1069
RsaI GTAC 1 cut(s) 761
RsaNI GTAC 1 cut(s) 760
RseI CAYNNNNRTG 2 cut(s) 362, 839
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 3 cut(s) 414, 663, 723
SatI GCNGC 9 cut(s) 169, 203, 274, 285, 288, 399, 402, 942, 1026
Sau96I GGNCC 4 cut(s) 571, 747, 1112, 1113
SchI GAGTC 1 cut(s) 1130
ScrFI CCNGG 7 cut(s) 223, 594, 745, 785, 1031, 1032, 1110
SduI GDGCHC 3 cut(s) 187, 1046, 1116
SfaNI GCATC 3 cut(s) 250, 646, 1081
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 2 cut(s) 571, 747
SmaI CCCGGG 1 cut(s) 1032
SmiMI CAYNNNNRTG 2 cut(s) 362, 839
SmlI CTYRAG 2 cut(s) 413, 1004
SmoI CTYRAG 2 cut(s) 413, 1004
Sse9I AATT 7 cut(s) 114, 309, 523, 579, 621, 814, 956
SspI AATATT 1 cut(s) 379
SspMI CTAG 2 cut(s) 132, 605
StyD4I CCNGG 7 cut(s) 221, 592, 743, 783, 1029, 1030, 1108
StyI CCWWGG 4 cut(s) 456, 547, 683, 909
TaaI ACNGT 3 cut(s) 841, 848, 974
TaiI ACGT 1 cut(s) 864
TaqI TCGA 7 cut(s) 229, 352, 610, 1021, 1131, 1139, 1155
TasI AATT 7 cut(s) 114, 309, 523, 579, 621, 814, 956
TatI WGTACW 1 cut(s) 759
TauI GCSGC 6 cut(s) 171, 276, 287, 290, 401, 404
TfiI GAWTC 3 cut(s) 226, 257, 354
Tru1I TTAA 3 cut(s) 414, 663, 723
Tru9I TTAA 3 cut(s) 414, 663, 723
TscAI CASTG 1 cut(s) 853
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 202, 941, 1025
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 2 cut(s) 517, 617
TspMI CCCGGG 1 cut(s) 1030
TspRI CASTG 1 cut(s) 853
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 571, 747
VspI ATTAAT 1 cut(s) 723
XapI RAATTY 1 cut(s) 621
XceI RCATGY 1 cut(s) 497
XmaI CCCGGG 1 cut(s) 1030
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 2 cut(s) 132, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.