MD16G1030200.v1.1

Xylosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016227
Physical Location & Seq
Reverse (-)
2159459 .. 2163615
4157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1030200.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGGGAGCTGAGAAGAAATGGCTCCTAACGCTCTTCTCAGCCACATTCCTCTCTCTCCTTCTCCTCCTTCTCTCCTCCATCTCCGCCTTCAGCTCCCCCAAACCCTTCCCTTCAATTGTCCAGCACGGCTCTCACTACCCGCCCGCTTTCGCTTACTACATATGGGGCGGCCGCGGCGATAGGGCTCGGATCTTCCGGCTGCTGCTCGCCGTCTACCATCCCCGAAATCGATACTTGTTGCATCTTTCGGCTGACGAATCTGAAGAAGAGCGCCGCCGCCTTGCCGCCGCTATCAAGGCTGTGCCTGCAATTCAGGCGTTTGGGAATGTGGATGTGGTGGGCAAGCCTGATCGAATCACTTACATGGGGTCCACAAATATTGCCACCACGTTGCGCGCGGCGGCGATTTTGTTGAAGGTTGATAGTGGGTGGGATTGGTTCGTCACTTTGAGCGCCATGGATTATCCACTTATCACCCAGGACGATCTGTCTCATGTATTCTCATCTGTCAGAAGAGATCTCAATTTTATTGATCACACTAGTGACCTTGGATGGAAAGAGTTGCATAGGGTCCAGCCAATAGTAGTTGATCCGGCAACATACCTAGCCAGAAGGAGCCAAATTTTTCATGCTACAGAGAAGCGGAAAACCCCTGATGCTTTTAAAGTATTCACAGGTTCCCCATGGGTCACTCTGAGCAGATCATTTCTGGAATTTTGCATTCTTGGTTGGGATAACCTTCCCCGGACCCTGCTCATGTACTTTACAAATGTGATATTGTCGCAGGAAGGATATTTCCATTCAGTTATATGCAATTCACCAGAGTACAAGAACACAACTGTGAACAGTGATCTAAGATACATGATCTGGGACACACCCCCAAAAATGGAACCCCACTTCCTCAACACCTCGGATTTTGATCAAATGATTCAAAGCGGAGCTGCTTTTGCCCGACAATTCGAGAAAGATGACCCTGTGATGGACATGGTCGATAAAAAGATCCTCAAGCGTGGGAGAAATCGAGCTGCCCCGGGGGCATGGTGCTCGGGCTGGAAAAGCTGGTGGATGGATCCTTGCACTCATTGGGGTGACGCCAATATCTTGAAGCCTGGGCCCCAAGCAAAGAAGTTTGAAGAGTCGATTACTAACCTTCTCGATGACTGGAATGCACAATCAAATCAATGCCAATGA

Protein Analysis

397

Amino Acids

45.25

Weight (kDa)

8.47

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 50 - 309 2e-66 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016227)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71070
fragaria_vesca FvH4_4g32220
malus_domestica MD13G1027100.v1.1 MD16G1030200.v1.1
prunus_persica Prupe.1G322800_v2.0.a1
pyrus_communis pycom13g02580 pycom16g02570
rosa_chinensis RchiOBHm_Chr4g0440961
rosa_laevigata RLG00000006136
rosa_multiflora Rmu_sc0000424.1_g000027
rosa_roxburghii Rroxscaffold_5G00381820
rosa_rugosa Rorug04G0329700
rosa_samantha Rh4AG381500 Rh4DG388000
rosa_wichuraiana Rw4G032860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 213
AccII CGCG 3 cut(s) 174, 396, 398
AclWI GGATC 5 cut(s) 197, 584, 994, 1064, 1077
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 2 cut(s) 621, 713
AcuI CTGAAG 2 cut(s) 73, 282
AcyI GRCGYC 1 cut(s) 1092
AfaI GTAC 2 cut(s) 761, 827
AfiI CCNNNNNNNGG 2 cut(s) 886, 979
AgsI TTSAA 5 cut(s) 114, 415, 932, 1105, 1133
AhdI GACNNNNNGTC 1 cut(s) 487
AhlI ACTAGT 1 cut(s) 539
AjnI CCWGG 2 cut(s) 477, 1108
AleI CACNNNNGTG 2 cut(s) 540, 839
AloI GAACNNNNNNTCC 2 cut(s) 882, 914
AluBI AGCT 5 cut(s) 8, 93, 941, 1025, 1059
AluI AGCT 5 cut(s) 8, 93, 941, 1025, 1059
Alw21I GWGCWC 1 cut(s) 1046
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 5 cut(s) 197, 584, 994, 1064, 1077
Ama87I CYCGRG 2 cut(s) 1030, 1045
AoxI GGCC 2 cut(s) 169, 1112
ApaI GGGCCC 1 cut(s) 1116
ApeKI GCWGC 4 cut(s) 199, 202, 941, 1025
ApoI RAATTY 2 cut(s) 621, 713
AspLEI GCGC 4 cut(s) 273, 396, 398, 455
AspS9I GGNCC 5 cut(s) 369, 571, 747, 1112, 1113
AsuC2I CCSGG 3 cut(s) 745, 1031, 1032
AsuHPI GGTGA 3 cut(s) 466, 810, 1100
AvaI CYCGRG 2 cut(s) 1030, 1045
AvaII GGWCC 3 cut(s) 369, 571, 747
BaeGI GKGCMC 1 cut(s) 1116
BamHI GGATCC 1 cut(s) 1069
BanII GRGCYC 2 cut(s) 187, 1116
Bbv12I GWGCWC 1 cut(s) 1046
BbvI GCAGC 4 cut(s) 186, 189, 928, 1012
BccI CCATC 5 cut(s) 86, 225, 546, 973, 1060
BceAI ACGGC 2 cut(s) 142, 194
BcgI CGANNNNNNTGC 2 cut(s) 1026, 1060
BciT130I CCWGG 2 cut(s) 479, 1110
BclI TGATCA 2 cut(s) 532, 919
BcnI CCSGG 3 cut(s) 745, 1031, 1032
BcoDI GTCTC 1 cut(s) 495
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 2 cut(s) 540, 605
BfmI CTRYAG 1 cut(s) 633
BfoI RGCGCY 2 cut(s) 274, 456
BglI GCCNNNNNGGC 1 cut(s) 1034
BglII AGATCT 1 cut(s) 517
Bme1390I CCNGG 5 cut(s) 479, 745, 1031, 1032, 1110
Bme18I GGWCC 3 cut(s) 369, 571, 747
BmeRI GACNNNNNGTC 1 cut(s) 487
BmeT110I CYCGRG 2 cut(s) 1030, 1045
BmgT120I GGNCC 5 cut(s) 369, 571, 747, 1112, 1113
BmrFI CCNGG 5 cut(s) 479, 745, 1031, 1032, 1110
BmsI GCATC 2 cut(s) 250, 646
BpuEI CTTGAG 1 cut(s) 989
BpuMI CCSGG 3 cut(s) 745, 1031, 1032
Bsa29I ATCGAT 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 1092
Bsc4I CCNNNNNNNGG 2 cut(s) 886, 979
Bse1I ACTGG 1 cut(s) 1166
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 2 cut(s) 479, 1110
BseCI ATCGAT 1 cut(s) 229
BseGI GGATG 4 cut(s) 217, 337, 557, 1071
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 2 cut(s) 886, 979
BseMII CTCAG 2 cut(s) 51, 686
BseNI ACTGG 1 cut(s) 1166
BsePI GCGCGC 1 cut(s) 394
BseRI GAGGAG 2 cut(s) 53, 64
BseSI GKGCMC 1 cut(s) 1116
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 4 cut(s) 186, 189, 928, 1012
Bsh1236I CGCG 3 cut(s) 174, 396, 398
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 2 cut(s) 171, 1114
BshVI ATCGAT 1 cut(s) 229
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 1046
BsiHKCI CYCGRG 2 cut(s) 1030, 1045
BsiSI CCGG 4 cut(s) 196, 593, 745, 1031
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 2 cut(s) 886, 979
BsmAI GTCTC 1 cut(s) 495
BsmFI GGGAC 1 cut(s) 884
BsmI GAATGC 2 cut(s) 720, 1171
BsnI GGCC 2 cut(s) 171, 1114
BsoBI CYCGRG 2 cut(s) 1030, 1045
Bsp120I GGGCCC 1 cut(s) 1112
Bsp1286I GDGCHC 3 cut(s) 187, 1046, 1116
Bsp19I CCATGG 2 cut(s) 456, 683
BspANI GGCC 2 cut(s) 171, 1114
BspCNI CTCAG 2 cut(s) 50, 687
BspDI ATCGAT 1 cut(s) 229
BspFNI CGCG 3 cut(s) 174, 396, 398
BspPI GGATC 5 cut(s) 197, 584, 994, 1064, 1077
BspQI GCTCTTC 2 cut(s) 38, 261
BsrI ACTGG 1 cut(s) 1166
BssHII GCGCGC 1 cut(s) 394
BssNI GRCGYC 1 cut(s) 1092
BssT1I CCWWGG 3 cut(s) 456, 547, 683
Bst2UI CCWGG 2 cut(s) 479, 1110
Bst4CI ACNGT 2 cut(s) 841, 848
Bst6I CTCTTC 4 cut(s) 38, 261, 508, 1128
BstACI GRCGYC 1 cut(s) 1092
BstC8I GCNNGC 5 cut(s) 144, 207, 306, 344, 396
BstDEI CTNAG 4 cut(s) 9, 37, 695, 854
BstDSI CCRYGG 3 cut(s) 172, 456, 683
BstF5I GGATG 4 cut(s) 217, 337, 557, 1071
BstFNI CGCG 3 cut(s) 174, 396, 398
BstH2I RGCGCY 2 cut(s) 274, 456
BstHHI GCGC 4 cut(s) 273, 396, 398, 455
BstMAI GTCTC 1 cut(s) 495
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 8 cut(s) 28, 174, 296, 305, 314, 947, 1034, 1056
BstNI CCWGG 2 cut(s) 479, 1110
BstSCI CCNGG 5 cut(s) 477, 743, 1029, 1030, 1108
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 1 cut(s) 1116
BstUI CGCG 3 cut(s) 174, 396, 398
BstV1I GCAGC 4 cut(s) 186, 189, 928, 1012
BstX2I RGATCY 4 cut(s) 189, 517, 999, 1069
BstYI RGATCY 4 cut(s) 189, 517, 999, 1069
BstZI CGGCCG 1 cut(s) 169
Bsu15I ATCGAT 1 cut(s) 229
BsuRI GGCC 2 cut(s) 171, 1114
BsuTUI ATCGAT 1 cut(s) 229
BtgI CCRYGG 3 cut(s) 172, 456, 683
BtsCI GGATG 4 cut(s) 217, 337, 557, 1071
BtsIMutI CAGTG 1 cut(s) 853
Cac8I GCNNGC 5 cut(s) 144, 207, 306, 344, 396
CciNI GCGGCCGC 1 cut(s) 169
CfoI GCGC 4 cut(s) 273, 396, 398, 455
Cfr13I GGNCC 5 cut(s) 369, 571, 747, 1112, 1113
Cfr42I CCGCGG 1 cut(s) 175
Cfr9I CCCGGG 1 cut(s) 1030
ClaI ATCGAT 1 cut(s) 229
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 2 cut(s) 760, 826
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 9 cut(s) 364, 457, 494, 629, 684, 757, 862, 985, 1038
CviQI GTAC 2 cut(s) 760, 826
DdeI CTNAG 4 cut(s) 9, 37, 695, 854
DraI TTTAAA 1 cut(s) 664
DriI GACNNNNNGTC 1 cut(s) 487
EaeI YGGCCR 1 cut(s) 169
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 4 cut(s) 38, 261, 508, 1128
Eam1105I GACNNNNNGTC 1 cut(s) 487
EarI CTCTTC 4 cut(s) 38, 261, 508, 1128
EciI GGCGGA 1 cut(s) 73
EclXI CGGCCG 1 cut(s) 169
Eco130I CCWWGG 3 cut(s) 456, 547, 683
Eco24I GRGCYC 2 cut(s) 187, 1116
Eco47I GGWCC 3 cut(s) 369, 571, 747
Eco52I CGGCCG 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 73, 282
Eco88I CYCGRG 2 cut(s) 1030, 1045
EcoO109I RGGNCCY 1 cut(s) 1113
EcoRII CCWGG 2 cut(s) 477, 1108
EcoT14I CCWWGG 3 cut(s) 456, 547, 683
EcoT38I GRGCYC 2 cut(s) 187, 1116
ErhI CCWWGG 3 cut(s) 456, 547, 683
FaeI CATG 9 cut(s) 367, 460, 497, 632, 687, 760, 865, 988, 1041
FaqI GGGAC 1 cut(s) 884
FatI CATG 9 cut(s) 363, 456, 493, 628, 683, 756, 861, 984, 1037
FauI CCCGC 2 cut(s) 147, 151
FauNDI CATATG 1 cut(s) 161
FbaI TGATCA 2 cut(s) 532, 919
FblI GTMKAC 1 cut(s) 213
FokI GGATG 4 cut(s) 204, 344, 564, 1078
FriOI GRGCYC 2 cut(s) 187, 1116
FspBI CTAG 2 cut(s) 540, 605
GlaI GCGC 4 cut(s) 272, 395, 397, 454
HaeII RGCGCY 2 cut(s) 274, 456
HaeIII GGCC 2 cut(s) 171, 1114
HapII CCGG 4 cut(s) 196, 593, 745, 1031
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 4 cut(s) 273, 396, 398, 455
Hin1I GRCGYC 1 cut(s) 1092
Hin1II CATG 9 cut(s) 367, 460, 497, 632, 687, 760, 865, 988, 1041
Hin6I GCGC 4 cut(s) 271, 394, 396, 453
HinP1I GCGC 4 cut(s) 271, 394, 396, 453
HinfI GANTC 4 cut(s) 257, 354, 928, 1136
HpaII CCGG 4 cut(s) 196, 593, 745, 1031
HphI GGTGA 3 cut(s) 466, 810, 1100
Hpy166II GTNNAC 3 cut(s) 214, 372, 844
Hpy188I TCNGA 5 cut(s) 189, 262, 512, 696, 913
Hpy188III TCNNGA 4 cut(s) 710, 961, 1102, 1154
Hpy8I GTNNAC 3 cut(s) 214, 372, 844
HpyCH4III ACNGT 2 cut(s) 841, 848
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 7 cut(s) 241, 308, 565, 720, 813, 1077, 1169
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 174, 296, 305, 314, 947, 1034, 1056
HpyF3I CTNAG 4 cut(s) 9, 37, 695, 854
HpySE526I ACGT 1 cut(s) 389
Hsp92I GRCGYC 1 cut(s) 1092
Hsp92II CATG 9 cut(s) 367, 460, 497, 632, 687, 760, 865, 988, 1041
HspAI GCGC 4 cut(s) 271, 394, 396, 453
Ksp22I TGATCA 2 cut(s) 532, 919
KspI CCGCGG 1 cut(s) 175
LguI GCTCTTC 2 cut(s) 38, 261
LmnI GCTCC 5 cut(s) 5, 27, 98, 615, 938
Lsp1109I GCAGC 4 cut(s) 186, 189, 928, 1012
LweI GCATC 2 cut(s) 250, 646
MaeI CTAG 2 cut(s) 540, 605
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 4 cut(s) 442, 542, 688, 1088
MboII GAAGA 7 cut(s) 25, 25, 184, 275, 278, 525, 1145
MfeI CAATTG 1 cut(s) 114
MflI RGATCY 4 cut(s) 189, 517, 999, 1069
MhlI GDGCHC 3 cut(s) 187, 1046, 1116
MluCI AATT 7 cut(s) 114, 309, 523, 621, 713, 814, 956
MlyI GAGTC 1 cut(s) 1145
MnlI CCTC 6 cut(s) 59, 74, 85, 911, 919, 1013
MseI TTAA 1 cut(s) 663
MslI CAYNNNNRTG 4 cut(s) 362, 540, 839, 1086
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 4 cut(s) 196, 593, 745, 1031
MspR9I CCNGG 5 cut(s) 479, 745, 1031, 1032, 1110
MunI CAATTG 1 cut(s) 114
Mva1269I GAATGC 2 cut(s) 720, 1171
MvaI CCWGG 2 cut(s) 479, 1110
MvnI CGCG 3 cut(s) 174, 396, 398
MwoI GCNNNNNNNGC 8 cut(s) 28, 174, 296, 305, 314, 947, 1034, 1056
NciI CCSGG 3 cut(s) 745, 1031, 1032
NcoI CCATGG 2 cut(s) 456, 683
NdeI CATATG 1 cut(s) 161
NlaIII CATG 9 cut(s) 367, 460, 497, 632, 687, 760, 865, 988, 1041
NmuCI GTSAC 4 cut(s) 442, 542, 688, 1088
NotI GCGGCCGC 1 cut(s) 169
OliI CACNNNNGTG 2 cut(s) 540, 839
PauI GCGCGC 1 cut(s) 394
PciSI GCTCTTC 2 cut(s) 38, 261
PctI GAATGC 2 cut(s) 720, 1171
PfeI GAWTC 3 cut(s) 257, 354, 928
PflFI GACNNNGTC 1 cut(s) 986
PleI GAGTC 1 cut(s) 1144
PpsI GAGTC 1 cut(s) 1144
Psp6I CCWGG 2 cut(s) 477, 1108
PspGI CCWGG 2 cut(s) 477, 1108
PspOMI GGGCCC 1 cut(s) 1112
PspPI GGNCC 5 cut(s) 369, 571, 747, 1112, 1113
PsuI RGATCY 4 cut(s) 189, 517, 999, 1069
PsyI GACNNNGTC 1 cut(s) 986
PteI GCGCGC 1 cut(s) 394
RsaI GTAC 2 cut(s) 761, 827
RsaNI GTAC 2 cut(s) 760, 826
RseI CAYNNNNRTG 4 cut(s) 362, 540, 839, 1086
SacII CCGCGG 1 cut(s) 175
SapI GCTCTTC 2 cut(s) 38, 261
SaqAI TTAA 1 cut(s) 663
Sau96I GGNCC 5 cut(s) 369, 571, 747, 1112, 1113
SchI GAGTC 1 cut(s) 1145
ScrFI CCNGG 5 cut(s) 479, 745, 1031, 1032, 1110
SduI GDGCHC 3 cut(s) 187, 1046, 1116
SfaNI GCATC 2 cut(s) 250, 646
SfcI CTRYAG 1 cut(s) 633
Sfr303I CCGCGG 1 cut(s) 175
SgrBI CCGCGG 1 cut(s) 175
SinI GGWCC 3 cut(s) 369, 571, 747
SmaI CCCGGG 1 cut(s) 1032
SmiMI CAYNNNNRTG 4 cut(s) 362, 540, 839, 1086
SmlI CTYRAG 1 cut(s) 1004
SmoI CTYRAG 1 cut(s) 1004
SpeI ACTAGT 1 cut(s) 539
Sse9I AATT 7 cut(s) 114, 309, 523, 621, 713, 814, 956
SspI AATATT 1 cut(s) 379
SspMI CTAG 2 cut(s) 540, 605
StyD4I CCNGG 5 cut(s) 477, 743, 1029, 1030, 1108
StyI CCWWGG 3 cut(s) 456, 547, 683
TaaI ACNGT 2 cut(s) 841, 848
TaiI ACGT 1 cut(s) 392
TaqI TCGA 7 cut(s) 229, 352, 960, 990, 1021, 1139, 1155
TasI AATT 7 cut(s) 114, 309, 523, 621, 713, 814, 956
TatI WGTACW 2 cut(s) 759, 825
TauI GCSGC 9 cut(s) 171, 174, 177, 276, 279, 287, 290, 401, 404
TfiI GAWTC 3 cut(s) 257, 354, 928
Tru1I TTAA 1 cut(s) 663
Tru9I TTAA 1 cut(s) 663
TscAI CASTG 1 cut(s) 853
TseFI GTSAC 4 cut(s) 442, 542, 688, 1088
TseI GCWGC 4 cut(s) 199, 202, 941, 1025
Tsp45I GTSAC 4 cut(s) 442, 542, 688, 1088
TspDTI ATGAA 1 cut(s) 617
TspMI CCCGGG 1 cut(s) 1030
TspRI CASTG 1 cut(s) 853
Tth111I GACNNNGTC 1 cut(s) 986
VpaK11BI GGWCC 3 cut(s) 369, 571, 747
XapI RAATTY 2 cut(s) 621, 713
XmaI CCCGGG 1 cut(s) 1030
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 2 cut(s) 540, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.