MD13G1041700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015056
Physical Location & Seq
Forward (+)
2861639 .. 2862034
396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1041700.v1.1.491

Sequence Viewer

Length: 396 bp
ATGAAGAGGCGGAGACGTAGTGGCGGAGGCGGCGCCGGTGTTGATCAAGCTCCGAGTTTTGAAGTTACAACGACAAAAACAGTGGCGGCTGCGAGCAAGAAGGGTAAGAAAACTGTCGACAAGAGCGTGAAAGAAGAGGCGAGGATCGACGGCGGCAATGATCAGCAATTGGGTTTGGTAGCGGCTGATCAGAATCTTATGGCTGGGGATGAGAATTACTGGGGGAATTTGGGTGGTGGTGTTGCGGTGGATGAGCAAATGTCGTGGGGTTCCGTTTGGTTGCCACTTTGGGATGTTGAGTTTATGGAAGAGTCGCATTACCGATTGTTTAGTGATGTGATTTGGGACGATGACATTTGGGGATTAAGAACCACAAAAGAGATCCCAAATCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.43

Weight (kDa)

4.87

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015056)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25422 AT1G68500
fragaria_vesca FvH4_4g27340
malus_domestica MD13G1041700.v1.1 MD16G1042600.v1.1
prunus_persica Prupe.1G311500_v2.0.a1
pyrus_communis pycom13g03610
rosa_chinensis RchiOBHm_Chr4g0435141
rosa_laevigata RLG00000006616
rosa_multiflora Rmu_ssc0000339.1_g000038
rosa_roxburghii Rroxscaffold_5G00376360
rosa_rugosa Rorug04G0282600
rosa_samantha Rh4BG346400 Rh4CG360900 Rh4DG340800
rosa_wichuraiana Rw4G029490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 117
AciI CCGC 7 cut(s) 10, 24, 30, 86, 153, 182, 245
AclWI GGATC 2 cut(s) 152, 376
AcsI RAATTY 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 33
AgsI TTSAA 1 cut(s) 62
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 7
AlwI GGATC 2 cut(s) 152, 376
ApeKI GCWGC 1 cut(s) 89
ApoI RAATTY 1 cut(s) 226
AspLEI GCGC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 76
BceAI ACGGC 1 cut(s) 166
BclI TGATCA 3 cut(s) 43, 160, 187
BcoDI GTCTC 1 cut(s) 7
BfoI RGCGCY 1 cut(s) 36
BisI GCNGC 5 cut(s) 31, 87, 90, 154, 183
BlsI GCNGC 5 cut(s) 32, 88, 91, 155, 184
BmiI GGNNCC 2 cut(s) 34, 271
BmrI ACTGGG 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 33
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 224
Bse3DI GCAATG 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 192
BseGI GGATG 3 cut(s) 214, 256, 298
BseJI GATNNNNATC 1 cut(s) 192
BseMI GCAATG 1 cut(s) 163
BseNI ACTGG 1 cut(s) 224
BseXI GCAGC 1 cut(s) 76
BseYI CCCAGC 1 cut(s) 203
BshNI GGYRCC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 359
BsmAI GTCTC 1 cut(s) 7
BsmBI CGTCTC 1 cut(s) 7
BsmFI GGGAC 1 cut(s) 359
Bsp143I GATC 5 cut(s) 43, 144, 160, 187, 381
BspACI CCGC 7 cut(s) 10, 24, 30, 86, 153, 182, 245
BspLI GGNNCC 2 cut(s) 34, 271
BspPI GGATC 2 cut(s) 152, 376
BspT107I GGYRCC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 224
BssAI RCCGGY 1 cut(s) 35
BssMI GATC 5 cut(s) 43, 144, 160, 187, 381
BssNI GRCGYC 1 cut(s) 33
Bst4CI ACNGT 2 cut(s) 82, 115
Bst6I CTCTTC 2 cut(s) 129, 303
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 94
BstF5I GGATG 3 cut(s) 214, 256, 298
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 5 cut(s) 46, 147, 163, 190, 384
BstMAI GTCTC 1 cut(s) 7
BstMBI GATC 5 cut(s) 43, 144, 160, 187, 381
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BtsCI GGATG 3 cut(s) 214, 256, 298
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 94
CfoI GCGC 1 cut(s) 35
Cfr10I RCCGGY 1 cut(s) 35
CspCI CAANNNNNGTGG 4 cut(s) 63, 98, 245, 280
CviJI RGCY 4 cut(s) 50, 89, 185, 203
CviKI_1 RGCY 4 cut(s) 50, 89, 185, 203
DinI GGCGCC 1 cut(s) 34
DpnI GATC 5 cut(s) 45, 146, 162, 189, 383
DpnII GATC 5 cut(s) 43, 144, 160, 187, 381
Eam1104I CTCTTC 2 cut(s) 129, 303
EarI CTCTTC 2 cut(s) 129, 303
EciI GGCGGA 2 cut(s) 25, 39
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
Esp3I CGTCTC 1 cut(s) 7
FaiI YATR 2 cut(s) 200, 305
FaqI GGGAC 1 cut(s) 359
FbaI TGATCA 3 cut(s) 43, 160, 187
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 5 cut(s) 31, 87, 90, 154, 183
FokI GGATG 3 cut(s) 221, 263, 305
Fsp4HI GCNGC 5 cut(s) 31, 87, 90, 154, 183
GlaI GCGC 1 cut(s) 34
GluI GCNGC 5 cut(s) 31, 87, 90, 154, 183
GsaI CCCAGC 1 cut(s) 207
HaeII RGCGCY 1 cut(s) 36
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 35
Hin1I GRCGYC 1 cut(s) 33
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HinfI GANTC 2 cut(s) 193, 311
HpaII CCGG 1 cut(s) 36
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 2 cut(s) 54, 192
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 82, 115
HpyCH4IV ACGT 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpySE526I ACGT 1 cut(s) 16
Hsp92I GRCGYC 1 cut(s) 33
HspAI GCGC 1 cut(s) 33
KasI GGCGCC 1 cut(s) 32
Ksp22I TGATCA 3 cut(s) 43, 160, 187
Kzo9I GATC 5 cut(s) 43, 144, 160, 187, 381
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 3 cut(s) 49, 189, 205
Lsp1109I GCAGC 1 cut(s) 76
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 64
MalI GATC 5 cut(s) 45, 146, 162, 189, 383
MboI GATC 5 cut(s) 43, 144, 160, 187, 381
MboII GAAGA 3 cut(s) 16, 146, 320
MfeI CAATTG 1 cut(s) 167
MflI RGATCY 1 cut(s) 381
MluCI AATT 3 cut(s) 167, 214, 226
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 1 cut(s) 320
MnlI CCTC 3 cut(s) 20, 130, 135
MseI TTAA 1 cut(s) 365
MspI CCGG 1 cut(s) 36
MunI CAATTG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 30
NarI GGCGCC 1 cut(s) 33
NdeII GATC 5 cut(s) 43, 144, 160, 187, 381
NlaIV GGNNCC 2 cut(s) 34, 271
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 5 cut(s) 32, 88, 91, 155, 184
PleI GAGTC 1 cut(s) 319
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 319
PspFI CCCAGC 1 cut(s) 203
PspN4I GGNNCC 2 cut(s) 34, 271
PsuI RGATCY 1 cut(s) 381
SalI GTCGAC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 5 cut(s) 31, 87, 90, 154, 183
Sau3AI GATC 5 cut(s) 43, 144, 160, 187, 381
SchI GAGTC 1 cut(s) 320
SetI ASST 2 cut(s) 19, 52
SfoI GGCGCC 1 cut(s) 34
SgrAI CRCCGGYG 1 cut(s) 35
Sse9I AATT 3 cut(s) 167, 214, 226
SsiI CCGC 7 cut(s) 10, 24, 30, 86, 153, 182, 245
SspDI GGCGCC 1 cut(s) 32
TaaI ACNGT 2 cut(s) 82, 115
TaiI ACGT 1 cut(s) 19
TaqI TCGA 2 cut(s) 117, 147
TasI AATT 3 cut(s) 167, 214, 226
TauI GCSGC 4 cut(s) 33, 89, 156, 185
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TscAI CASTG 1 cut(s) 87
TseI GCWGC 1 cut(s) 89
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 262, 381
TspRI CASTG 1 cut(s) 87
XapI RAATTY 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.