Rorug04G0282600

RuBisCO catalyzes two reactions the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002444
Physical Location & Seq
Forward (+)
45665604 .. 45672189
6586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0282600.1

Sequence Viewer

Length: 3096 bp
ATGGCAGATTTGGACGACGATTTTGGAGACATCTACACTGATGTCGAAGCGCAGGCCGCTTCAGCCATCAACGGCGTATCGGACTTCGCTCAGTTCTATACAGAACCCGAAGAAGATAGAAATGATAGTAACAACAATGCAAAGAACGGCGACACTTGCTCCGGCGAGGGTTTCGTCTCCGATTCTAAGAAACTCAAAGAGGAATTGGGTGGTAAAGTTTCGGGTTCGGAGTCGGATGAGGATTACAGCGACAGTGACAGTGAGGATGACCTGAATATAGTTTTGAACGATGGGGGCTGTAAAGGTATACCCCTTCCGGTTTCAGGTGGAGGGGGCATGAGGAGGATTGATGATTATGAAGATGAGGATGATGATGCTCTTGTGCCCATGAAAGATTGCAAGTATGTATGGACTCAAGGATCTACTTGTCCAAGCAATGGAAAAGCCAATGAGTCTGTGGATTTGGTGTCGTCCATGCCGTTTGGAGTAGACCACTCGCAGATTAGTCGTGTTCGTGATGCACCTAATCCAGTGGTGACTCGAAATAGATATGGTTTTCGACTTCCTTGGTATAGGACCATACTGGACGTGGATATTGAATCATTTGAGGAGAAGTCATGGAGGTATCCTGGAGTTGATATAACAGATTACTTCAATTTTGATTTGAACGAGGACAGCTGGAAAGAGTACTGCAACTCCCTGGAACAACTTCGGCAGCTCAAATCCACACAGTATGGCATGCCTGGCTCTTCAAAATTTTATCAGGCTAAAGAAGCTGGATATGAGTATGGCAGATTCACCCAGGAAATGAATTATGTTGGATCAAGCACATATGCTCCTCCGTCATCAACAGTTTCTGATTGCAGACCAAAAGGGAAAGCAATTCAGATAGAAGATAGTATTGTTGAACGCCAACCATCTTTTGATAGCAGGCATCCACGTAGTTGGAATTCTGATGTTGTGATACAGATTTCTGTGCAGGATCCCACCCAGGAAACCTCCAATTCTGGCGAGGAACATGGGCATACAAATAGCAGGGCCCATGAGACATCTGAAAATGCGGAGTTTGATGCAAATGAAAACCAAAATAATATGTCATATAATACTACAAACAATGATGATTCATCATTAGGAAGTCCAGAAGCAAACACAAGAAAATTAGACAGGTGTTCCCAGAAGATGAGTGCATCCCATCTAATGACAATAGACTCTGATGATCACAAAAATAACCAGGTCGTTGATGTGGATGGGAATAACCATAAGGTAGTGAATGGTATCTCATTGGAGGCCATTGAAATTGCAAATAAAGTGACAGAAAGTCTTGACAGGAATACTTCCTGTGCTGATCAATGCATGGTGGAGACGCAATTATCGCTGAGTGATGATGATGATCAACTTAGTCTCATTTCATCATGTTTTGCATCTGATTCTGAAGCTTCCAAGAACAGCGTTCATTTTGATCCAGAAGATATTCATACTCCTGTAAGGCCTGTCGTGAATTCACACACTGAACCACGCAAGTCTGTCACATCGTCTTTTAAGAACTCGAAAAGCAATTACATCAAGAGAAAAAGAGTTGATATTCAAGATTATTCCATCTGTAGAAGTTCTTCTCAAAAGAAGCATAATCATCAGGCCAGGAGTCTTAATGGTGTTGATGAACTGAGTAATCACAGAAACAACGACAATGATGGGTCACCCACCTCAGATAGAGAGGACCCTTATAACAGGAATTGCTCAGAGAATTTTCATGGACAGAAGGACAGGCTGCAAGATTCTGGCCTTATTAATAGAGAAGATAGTTCAGATTATAAAGAACCAAAGTTTTCCTGCCATTATGGTATTAGATATGATGATTACCATGATCAGAATCAAAGAGATAAGAGAAATTATTCAAGCAGAAAAGGTAGTCACCAGTTCCAAGAGAAACTAGATCCATATGCGAAAAGAACTTTCAATGAAAGGCAGACTTTTAGTGAAGATACGGATAGAGATTGGAGTCACTTTGGAAGAACGCAGTTCACTGAGGAGAGGAGTTCTCTTCCCAGCAGGGAGGCCAGGGGGTTGCATTCTAGGTATTCCTCCACTGTTGAAGAAAGAAATGCTCAATGGAGAAGGAACAGCAATAAACTGCTATTTAAGAAAATAACCAATTATAAGTGGCATGAAGATGATTTTCTGGGAGAAAAATCTGGGAGATGCGCATCATTCACTGGTCGAAAAAGGAATAATTTTGATGAATTTGATGAAAGGCAGTTGCCACCTGTTGGGAGAGAATTAAGTAACTGGGAGCAAAGGGGTGGATATGTGGACAGTCCCATTTATTTGGATGATTCACACTCTGAGAAATTTGAAGATGAGTATTGCAAACGCAGAGAAAATCGATACTTGTCCCATCAGTCTTACAGAGACTTATATACAGGTGACGAAGGAAGCTGGAATCACTCCATATCACCAAGAAATAATGCTTCTTACCCAAGAAGAGCTGATGAAAGATATTGGAGACATGTGAGAAAAATAGATGCCGAAGAAGCCAATGAGACTAATTGGTTTGATGATCACTATGATGCTTATGAAATCGAAGATAACATGTATCTTAATGATCATTTAAGGTGGAGAAGATCTAACTGGCGATCTGAAGTTATGCACTGGACTGAAGATCAGTTTACTTTCAGACATCATGGTGATGAATTGTATTCTGAGAAACCATCCTGTTCTTATCGGAAATATGTACGGCGTGAAAAATTTCATGCTAAATATGGTCTTTTGCGTGATGGAATGCCTACAGATATTATGCAGCCAGAGCAGCATAGATTGAAAATACCCAGAAAAGGAATTGGTGCTAACTTTATTAATAGAAGTGCTAAGATGTATAGAGGTAAGCATGAGCAGACGGTGAGGTGCAGGGATTCGATGGACTTGGCTGTTAGGGAACGAAAGGTAAAGTTGGGGAAACCAAGGGTGAAACCTTTCATCACCCTACTTGTATATATGAGTGCTCCAATTCAATCTCTGCAAGATGCTCCAAAGCCAGAATTTCGATGTGCAATGGTAGGCCAGAAAGTATGGGTGCAGAGATTGGTGGAGAGGGGATAA

Protein Analysis

1031

Amino Acids

119.16

Weight (kDa)

5.44

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fip1 PF05182 196 - 238 8.4e-20 Fip1 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1725, 1812, 2157
Acc16I TGCGCA 1 cut(s) 2203
AccB7I CCANNNNNTGG 2 cut(s) 437, 2267
AccI GTMKAC 2 cut(s) 307, 489
AciI CCGC 2 cut(s) 57, 1061
AclWI GGATC 6 cut(s) 427, 829, 977, 990, 1454, 1928
AcsI RAATTY 8 cut(s) 755, 949, 1498, 1744, 2240, 2348, 2744, 3035
AcuI CTGAAG 4 cut(s) 45, 1452, 2658, 2676
AfaI GTAC 2 cut(s) 689, 2733
AfiI CCNNNNNNNGG 4 cut(s) 323, 437, 2267, 2831
AflIII ACRYGT 2 cut(s) 2506, 2589
AhdI GACNNNNNGTC 1 cut(s) 1317
AjiI CACGTC 1 cut(s) 589
AjnI CCWGG 8 cut(s) 628, 699, 742, 801, 990, 1230, 1637, 2057
AluBI AGCT 6 cut(s) 678, 718, 776, 1436, 2436, 2486
AluI AGCT 6 cut(s) 678, 718, 776, 1436, 2436, 2486
Alw21I GWGCWC 1 cut(s) 3001
Alw26I GTCTC 8 cut(s) 21, 181, 1040, 1355, 1406, 2403, 2497, 2534
AlwI GGATC 6 cut(s) 427, 829, 977, 990, 1454, 1928
AlwNI CAGNNNCTG 1 cut(s) 857
AoxI GGCC 8 cut(s) 54, 1038, 1287, 1487, 1635, 1780, 2055, 3055
ApaI GGGCCC 1 cut(s) 1042
ApeKI GCWGC 4 cut(s) 715, 1768, 2797, 2806
ApoI RAATTY 8 cut(s) 755, 949, 1498, 1744, 2240, 2348, 2744, 3035
ArsI GACNNNNNNTTYG 1 cut(s) 37
AseI ATTAAT 2 cut(s) 1788, 2853
Asp700I GAANNNNTTC 6 cut(s) 708, 1470, 1609, 1891, 2745, 2969
AspLEI GCGC 2 cut(s) 52, 2204
AspS9I GGNCC 4 cut(s) 576, 1038, 1039, 1717
AvaII GGWCC 2 cut(s) 576, 1717
BaeGI GKGCMC 2 cut(s) 387, 1042
BamHI GGATCC 1 cut(s) 982
BanII GRGCYC 1 cut(s) 1042
BarI GAAGNNNNNNTAC 2 cut(s) 1594, 1626
Bbv12I GWGCWC 1 cut(s) 3001
BbvI GCAGC 4 cut(s) 727, 1755, 2809, 2818
BceAI ACGGC 4 cut(s) 88, 163, 463, 2750
BcgI CGANNNNNNTGC 2 cut(s) 488, 522
BciT130I CCWGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
BciVI GTATCC 1 cut(s) 636
BclI TGATCA 6 cut(s) 1216, 1345, 1390, 1864, 2557, 2602
BcoDI GTCTC 8 cut(s) 21, 181, 1040, 1355, 1406, 2403, 2497, 2534
BfaI CTAG 2 cut(s) 1931, 2073
BfmI CTRYAG 2 cut(s) 1600, 2784
BfuI GTATCC 1 cut(s) 636
BglII AGATCT 1 cut(s) 2621
BisI GCNGC 5 cut(s) 57, 716, 1769, 2798, 2807
BlsI GCNGC 5 cut(s) 58, 717, 1770, 2799, 2808
BmcAI AGTACT 1 cut(s) 689
Bme1390I CCNGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
Bme18I GGWCC 2 cut(s) 576, 1717
BmeRI GACNNNNNGTC 1 cut(s) 1317
BmgBI CACGTC 1 cut(s) 589
BmgT120I GGNCC 4 cut(s) 576, 1038, 1039, 1717
BmiI GGNNCC 3 cut(s) 984, 1040, 1719
BmrFI CCNGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
BmrI ACTGGG 1 cut(s) 2296
BmuI ACTGGG 1 cut(s) 2296
BplI GAGNNNNNCTC 2 cut(s) 2023, 2055
BpmI CTGGAG 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 399
Bsa29I ATCGAT 1 cut(s) 2383
BsaAI YACGTR 1 cut(s) 941
BsaBI GATNNNNATC 3 cut(s) 1389, 1869, 2203
BsaJI CCNNGG 6 cut(s) 566, 699, 801, 990, 2058, 2957
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 6 cut(s) 143, 173, 680, 710, 820, 850
Bsc4I CCNNNNNNNGG 4 cut(s) 323, 437, 2267, 2831
Bse1I ACTGG 7 cut(s) 530, 588, 1915, 2218, 2291, 2633, 2654
Bse3DI GCAATG 2 cut(s) 442, 3054
Bse8I GATNNNNATC 3 cut(s) 1389, 1869, 2203
BseBI CCWGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
BseCI ATCGAT 1 cut(s) 2383
BseDI CCNNGG 6 cut(s) 566, 699, 801, 990, 2058, 2957
BseGI GGATG 8 cut(s) 241, 271, 373, 934, 1187, 1252, 2335, 2708
BseJI GATNNNNATC 3 cut(s) 1389, 1869, 2203
BseLI CCNNNNNNNGG 4 cut(s) 323, 437, 2267, 2831
BseMI GCAATG 2 cut(s) 442, 3054
BseMII CTCAG 8 cut(s) 104, 1367, 1655, 1719, 1752, 2016, 2334, 2691
BseNI ACTGG 7 cut(s) 530, 588, 1915, 2218, 2291, 2633, 2654
BseRI GAGGAG 5 cut(s) 355, 623, 828, 2042, 2047
BseSI GKGCMC 2 cut(s) 387, 1042
BseXI GCAGC 4 cut(s) 727, 1755, 2809, 2818
BseYI CCCAGC 1 cut(s) 2045
BsgI GTGCAG 3 cut(s) 998, 2923, 3092
BshFI GGCC 8 cut(s) 56, 1040, 1289, 1489, 1637, 1782, 2057, 3057
BshVI ATCGAT 1 cut(s) 2383
BsiHKAI GWGCWC 1 cut(s) 3001
BsiSI CCGG 2 cut(s) 162, 317
BslFI GGGAC 2 cut(s) 2301, 2377
BslI CCNNNNNNNGG 4 cut(s) 323, 437, 2267, 2831
BsmAI GTCTC 8 cut(s) 21, 181, 1040, 1355, 1406, 2403, 2497, 2534
BsmBI CGTCTC 2 cut(s) 181, 1355
BsmFI GGGAC 2 cut(s) 2301, 2377
BsmI GAATGC 2 cut(s) 2068, 2784
BsnI GGCC 8 cut(s) 56, 1040, 1289, 1489, 1637, 1782, 2057, 3057
Bsp120I GGGCCC 1 cut(s) 1038
Bsp1286I GDGCHC 3 cut(s) 387, 1042, 3001
BspACI CCGC 2 cut(s) 57, 1061
BspANI GGCC 8 cut(s) 56, 1040, 1289, 1489, 1637, 1782, 2057, 3057
BspCNI CTCAG 8 cut(s) 103, 1368, 1656, 1718, 1751, 2017, 2335, 2692
BspDI ATCGAT 1 cut(s) 2383
BspLI GGNNCC 3 cut(s) 984, 1040, 1719
BspPI GGATC 6 cut(s) 427, 829, 977, 990, 1454, 1928
BspQI GCTCTTC 2 cut(s) 754, 2476
BsrDI GCAATG 2 cut(s) 442, 3054
BsrI ACTGG 7 cut(s) 530, 588, 1915, 2218, 2291, 2633, 2654
BssECI CCNNGG 6 cut(s) 566, 699, 801, 990, 2058, 2957
BssNAI GTATAC 1 cut(s) 308
BssT1I CCWWGG 2 cut(s) 566, 2957
Bst1107I GTATAC 1 cut(s) 308
Bst2UI CCWGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
Bst4CI ACNGT 7 cut(s) 254, 260, 732, 853, 2089, 2315, 2896
Bst6I CTCTTC 3 cut(s) 754, 2046, 2476
BstBAI YACGTR 1 cut(s) 941
BstC8I GCNNGC 3 cut(s) 54, 740, 932
BstEII GGTNACC 1 cut(s) 1695
BstF5I GGATG 8 cut(s) 241, 271, 373, 934, 1187, 1252, 2335, 2708
BstHHI GCGC 2 cut(s) 52, 2204
BstMAI GTCTC 8 cut(s) 21, 181, 1040, 1355, 1406, 2403, 2497, 2534
BstMWI GCNNNNNNNGC 9 cut(s) 56, 62, 156, 744, 773, 1372, 2531, 2803, 2806
BstNI CCWGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
BstNSI RCATGY 3 cut(s) 742, 2510, 2593
BstPI GGTNACC 1 cut(s) 1695
BstSCI CCNGG 8 cut(s) 628, 699, 742, 801, 990, 1230, 1637, 2057
BstSFI CTRYAG 2 cut(s) 1600, 2784
BstSLI GKGCMC 2 cut(s) 387, 1042
BstV1I GCAGC 4 cut(s) 727, 1755, 2809, 2818
BstX2I RGATCY 4 cut(s) 419, 982, 1933, 2621
BstXI CCANNNNNNTGG 2 cut(s) 945, 2326
BstYI RGATCY 4 cut(s) 419, 982, 1933, 2621
BstZ17I GTATAC 1 cut(s) 308
Bsu15I ATCGAT 1 cut(s) 2383
BsuI GTATCC 1 cut(s) 636
BsuRI GGCC 8 cut(s) 56, 1040, 1289, 1489, 1637, 1782, 2057, 3057
BsuTUI ATCGAT 1 cut(s) 2383
BtrI CACGTC 1 cut(s) 589
BtsCI GGATG 8 cut(s) 241, 271, 373, 934, 1187, 1252, 2335, 2708
BtsIMutI CAGTG 9 cut(s) 36, 259, 265, 537, 1506, 2022, 2085, 2211, 2647
Cac8I GCNNGC 3 cut(s) 54, 740, 932
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 2 cut(s) 52, 2204
Cfr13I GGNCC 4 cut(s) 576, 1038, 1039, 1717
ClaI ATCGAT 1 cut(s) 2383
CseI GACGC 1 cut(s) 1372
CsiI ACCWGGT 1 cut(s) 1230
Csp6I GTAC 2 cut(s) 688, 2732
CviQI GTAC 2 cut(s) 688, 2732
DriI GACNNNNNGTC 1 cut(s) 1317
Eam1104I CTCTTC 3 cut(s) 754, 2046, 2476
Eam1105I GACNNNNNGTC 1 cut(s) 1317
EarI CTCTTC 3 cut(s) 754, 2046, 2476
Eco130I CCWWGG 2 cut(s) 566, 2957
Eco147I AGGCCT 1 cut(s) 1489
Eco24I GRGCYC 1 cut(s) 1042
Eco47I GGWCC 2 cut(s) 576, 1717
Eco57I CTGAAG 4 cut(s) 45, 1452, 2658, 2676
Eco91I GGTNACC 1 cut(s) 1695
EcoO109I RGGNCCY 2 cut(s) 1038, 1717
EcoO65I GGTNACC 1 cut(s) 1695
EcoRI GAATTC 2 cut(s) 949, 1498
EcoRII CCWGG 8 cut(s) 628, 699, 742, 801, 990, 1230, 1637, 2057
EcoT14I CCWWGG 2 cut(s) 566, 2957
EcoT22I ATGCAT 1 cut(s) 1355
EcoT38I GRGCYC 1 cut(s) 1042
ErhI CCWWGG 2 cut(s) 566, 2957
Esp3I CGTCTC 2 cut(s) 181, 1355
FalI AAGNNNNNCTT 2 cut(s) 1954, 1986
FaqI GGGAC 2 cut(s) 2301, 2377
FauNDI CATATG 2 cut(s) 832, 1939
FbaI TGATCA 6 cut(s) 1216, 1345, 1390, 1864, 2557, 2602
FblI GTMKAC 2 cut(s) 307, 489
Fnu4HI GCNGC 5 cut(s) 57, 716, 1769, 2798, 2807
FokI GGATG 8 cut(s) 248, 278, 380, 921, 1174, 1259, 2342, 2695
FriOI GRGCYC 1 cut(s) 1042
Fsp4HI GCNGC 5 cut(s) 57, 716, 1769, 2798, 2807
FspAI RTGCGCAY 1 cut(s) 2203
FspBI CTAG 2 cut(s) 1931, 2073
FspI TGCGCA 1 cut(s) 2203
GlaI GCGC 2 cut(s) 51, 2203
GluI GCNGC 5 cut(s) 57, 716, 1769, 2798, 2807
GsaI CCCAGC 1 cut(s) 2049
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 8 cut(s) 56, 1040, 1289, 1489, 1637, 1782, 2057, 3057
HapII CCGG 2 cut(s) 162, 317
HgaI GACGC 1 cut(s) 1372
HhaI GCGC 2 cut(s) 52, 2204
Hin6I GCGC 2 cut(s) 50, 2202
HinP1I GCGC 2 cut(s) 50, 2202
HindIII AAGCTT 1 cut(s) 1434
HpaII CCGG 2 cut(s) 162, 317
Hpy166II GTNNAC 5 cut(s) 308, 490, 2022, 2311, 2667
Hpy188III TCNNGA 7 cut(s) 515, 1139, 1322, 1463, 1495, 1564, 1586
Hpy8I GTNNAC 5 cut(s) 308, 490, 2022, 2311, 2667
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 4 cut(s) 323, 1753, 2109, 2423
HpyCH4III ACNGT 7 cut(s) 254, 260, 732, 853, 2089, 2315, 2896
HpyCH4IV ACGT 2 cut(s) 588, 940
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 62, 156, 744, 773, 1372, 2531, 2803, 2806
HpySE526I ACGT 2 cut(s) 588, 940
HspAI GCGC 2 cut(s) 50, 2202
Ksp22I TGATCA 6 cut(s) 1216, 1345, 1390, 1864, 2557, 2602
LguI GCTCTTC 2 cut(s) 754, 2476
LmnI GCTCC 5 cut(s) 164, 841, 2290, 3004, 3028
Lsp1109I GCAGC 4 cut(s) 727, 1755, 2809, 2818
MabI ACCWGGT 1 cut(s) 1230
MaeI CTAG 2 cut(s) 1931, 2073
MaeII ACGT 2 cut(s) 588, 940
MflI RGATCY 4 cut(s) 419, 982, 1933, 2621
MhlI GDGCHC 3 cut(s) 387, 1042, 3001
MlyI GAGTC 7 cut(s) 239, 406, 461, 532, 1202, 1651, 2008
MmeI TCCRAC 3 cut(s) 213, 799, 926
Mph1103I ATGCAT 1 cut(s) 1355
MroXI GAANNNNTTC 6 cut(s) 708, 1470, 1609, 1891, 2745, 2969
MseI TTAA 8 cut(s) 1539, 1647, 1788, 2139, 2279, 2598, 2609, 2853
MslI CAYNNNNRTG 4 cut(s) 2169, 2565, 2682, 2685
MspA1I CMGCKG 1 cut(s) 678
MspI CCGG 2 cut(s) 162, 317
MspR9I CCNGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
Mva1269I GAATGC 2 cut(s) 2068, 2784
MvaI CCWGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
MwoI GCNNNNNNNGC 9 cut(s) 56, 62, 156, 744, 773, 1372, 2531, 2803, 2806
NdeI CATATG 2 cut(s) 832, 1939
NlaIV GGNNCC 3 cut(s) 984, 1040, 1719
NmuCI GTSAC 8 cut(s) 254, 535, 1309, 1525, 1695, 1910, 2000, 2423
NsbI TGCGCA 1 cut(s) 2203
NsiI ATGCAT 1 cut(s) 1355
NspI RCATGY 3 cut(s) 742, 2510, 2593
PaeI GCATGC 1 cut(s) 742
PceI AGGCCT 1 cut(s) 1489
PciI ACATGT 2 cut(s) 2506, 2589
PciSI GCTCTTC 2 cut(s) 754, 2476
PcsI WCGNNNNNNNCGW 1 cut(s) 476
PctI GAATGC 2 cut(s) 2068, 2784
PdmI GAANNNNTTC 6 cut(s) 708, 1470, 1609, 1891, 2745, 2969
PflMI CCANNNNNTGG 2 cut(s) 437, 2267
PfoI TCCNGGA 1 cut(s) 628
PkrI GCNGC 5 cut(s) 58, 717, 1770, 2799, 2808
PleI GAGTC 7 cut(s) 238, 406, 460, 532, 1202, 1650, 2007
PpsI GAGTC 7 cut(s) 238, 406, 460, 532, 1202, 1650, 2007
Ppu21I YACGTR 1 cut(s) 941
PpuMI RGGWCCY 1 cut(s) 1717
PscI ACATGT 2 cut(s) 2506, 2589
PshBI ATTAAT 2 cut(s) 1788, 2853
PsiI TTATAA 3 cut(s) 1725, 1812, 2157
Psp5II RGGWCCY 1 cut(s) 1717
Psp6I CCWGG 8 cut(s) 628, 699, 742, 801, 990, 1230, 1637, 2057
PspEI GGTNACC 1 cut(s) 1695
PspFI CCCAGC 1 cut(s) 2045
PspGI CCWGG 8 cut(s) 628, 699, 742, 801, 990, 1230, 1637, 2057
PspN4I GGNNCC 3 cut(s) 984, 1040, 1719
PspOMI GGGCCC 1 cut(s) 1038
PspPI GGNCC 4 cut(s) 576, 1038, 1039, 1717
PspPPI RGGWCCY 1 cut(s) 1717
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 4 cut(s) 419, 982, 1933, 2621
PvuII CAGCTG 1 cut(s) 678
RsaI GTAC 2 cut(s) 689, 2733
RsaNI GTAC 2 cut(s) 688, 2732
RseI CAYNNNNRTG 4 cut(s) 2169, 2565, 2682, 2685
SapI GCTCTTC 2 cut(s) 754, 2476
SaqAI TTAA 8 cut(s) 1539, 1647, 1788, 2139, 2279, 2598, 2609, 2853
SatI GCNGC 5 cut(s) 57, 716, 1769, 2798, 2807
Sau96I GGNCC 4 cut(s) 576, 1038, 1039, 1717
ScaI AGTACT 1 cut(s) 689
SchI GAGTC 7 cut(s) 239, 406, 461, 532, 1202, 1651, 2008
ScrFI CCNGG 8 cut(s) 630, 701, 744, 803, 992, 1232, 1639, 2059
SduI GDGCHC 3 cut(s) 387, 1042, 3001
SexAI ACCWGGT 1 cut(s) 1230
SfcI CTRYAG 2 cut(s) 1600, 2784
SinI GGWCC 2 cut(s) 576, 1717
SmiMI CAYNNNNRTG 4 cut(s) 2169, 2565, 2682, 2685
SmlI CTYRAG 1 cut(s) 414
SmoI CTYRAG 1 cut(s) 414
SphI GCATGC 1 cut(s) 742
SseBI AGGCCT 1 cut(s) 1489
SsiI CCGC 2 cut(s) 57, 1061
SspMI CTAG 2 cut(s) 1931, 2073
StuI AGGCCT 1 cut(s) 1489
StyD4I CCNGG 8 cut(s) 628, 699, 742, 801, 990, 1230, 1637, 2057
StyI CCWWGG 2 cut(s) 566, 2957
TaaI ACNGT 7 cut(s) 254, 260, 732, 853, 2089, 2315, 2896
TaiI ACGT 2 cut(s) 591, 943
TaqI TCGA 9 cut(s) 45, 541, 559, 1547, 2218, 2383, 2580, 2912, 3040
TatI WGTACW 1 cut(s) 687
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 8 cut(s) 1539, 1647, 1788, 2139, 2279, 2598, 2609, 2853
Tru9I TTAA 8 cut(s) 1539, 1647, 1788, 2139, 2279, 2598, 2609, 2853
TscAI CASTG 9 cut(s) 43, 259, 265, 537, 1513, 2029, 2092, 2218, 2654
TseFI GTSAC 8 cut(s) 254, 535, 1309, 1525, 1695, 1910, 2000, 2423
TseI GCWGC 4 cut(s) 715, 1768, 2797, 2806
Tsp45I GTSAC 8 cut(s) 254, 535, 1309, 1525, 1695, 1910, 2000, 2423
TspGWI ACGGA 2 cut(s) 831, 2000
TspRI CASTG 9 cut(s) 43, 259, 265, 537, 1513, 2029, 2092, 2218, 2654
Van91I CCANNNNNTGG 2 cut(s) 437, 2267
VpaK11BI GGWCC 2 cut(s) 576, 1717
VspI ATTAAT 2 cut(s) 1788, 2853
XapI RAATTY 8 cut(s) 755, 949, 1498, 1744, 2240, 2348, 2744, 3035
XceI RCATGY 3 cut(s) 742, 2510, 2593
XcmI CCANNNNNNNNNTGG 2 cut(s) 454, 586
XmiI GTMKAC 2 cut(s) 307, 489
XmnI GAANNNNTTC 6 cut(s) 708, 1470, 1609, 1891, 2745, 2969
XspI CTAG 2 cut(s) 1931, 2073
ZrmI AGTACT 1 cut(s) 689
Zsp2I ATGCAT 1 cut(s) 1355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.