Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002444

Physical Location & Seq

Genomic Coordinates Forward (+)

32385414 .. 32387041

Gene Structure 1628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1252100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 552 bp

ATGGCTTCCTCCATGATTTCCTCCGCTACCGTGTCTACAGTTTACGCCGACCGTGCCGCCCCGGCTCAAGCCAGCATGGTTGCTCCATTCACTGGCCTCAAGTCGTCCTCAGCTTTCCCTGTCACCAGAAAGAGCAACGACATTACCTCTATTGCAAGCAATGGAGGAAGAGTGCAATGTATGCAGGTGTGGCCTCCACTTGGATTGAAGAAGTTCGAGACCCTCTCTTACCTTCCTCCTCTCTCTTCCGAGTCCTTGGCCAAGGAAGTTGATTACCTCCTCCGCAAAAACTGGGTTCCCTGCTTGGAATTTGAGTTGGAGCATGGATTTGTGTACCGTGAGAACCACAAATCCCCAGGATACTACGATGGAAGGTACTGGACAATGTGGAAGCTGCCCATGTTCGGATGCACCGACTCTTCTCAGGTGTTGAAGGAGCTGGAGGAGGCCAAGAAGGCCTATCCCAACGCCTTCATCCGTATCATCGGATTCGACAACGTCCGCCAAGTGCAGTGCATCAGTTTCATCGCCTACAAGCCTGCAGGCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00630 ko00710 ko01100 ko01120 ko01200 map00630 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

RbcS RuBisCO_small

Protein Analysis

184

Amino Acids

20.52

Weight (kDa)

8.83

Isoelectric Point (pI)

35.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RbcS	PF12338	2 - 46	3.5e-22	Ribulose-1,5-bisphosphate carboxylase small subunit
RuBisCO_small	PF00101	72 - 180	1.8e-37	Ribulose bisphosphate carboxylase, small chain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002444)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67090 AT1G67090 AT5G38410 AT5G38410 AT5G38410 AT5G38420 AT5G38430 AT5G38430
fragaria_vesca	FvH4_4g27330 FvH4_6g26030
malus_domestica	MD09G1252100.v1.1 MD16G1042700.v1.1 MD17G1244400.v1.1
prunus_persica	Prupe.1G311400_v2.0.a1 Prupe.3G157200_v2.0.a1 Prupe.3G157200_v2.0.a1
pyrus_communis	pycom09g16870 pycom09g16880 pycom16g03670 pycom17g24720
rosa_chinensis	RchiOBHm_Chr2g0126111 RchiOBHm_Chr4g0435151
rosa_laevigata	RLG00000006615 RLG00000018888
rosa_multiflora	Rmu_co8187892.1_g000001 Rmu_sc0012196.1_g000003 Rmu_ssc0000339.1_g000037
rosa_roxburghii	Rroxscaffold_2G00117430 Rroxscaffold_5G00376370
rosa_rugosa	Rorug02G0261500 Rorug04G0282600
rosa_samantha	Rh2AG318400 Rh2BG326700 Rh2DG344900 Rh2DG346000 Rh4AG338200 Rh4BG346300 Rh4CG360800 Rh4DG340700
rosa_wichuraiana	Rw2G025780 Rw4G029500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	175
Acc36I	ACCTGC	1 cut(s)	175
AccB7I	CCANNNNNTGG	1 cut(s)	92
AccI	GTMKAC	1 cut(s)	35
AciI	CCGC	4 cut(s)	24, 57, 283, 502
AcoI	YGGCCR	1 cut(s)	258
AcsI	RAATTY	1 cut(s)	308
AfaI	GTAC	2 cut(s)	335, 377
AfiI	CCNNNNNNNGG	3 cut(s)	92, 200, 404
AgsI	TTSAA	2 cut(s)	208, 433
AjnI	CCWGG	1 cut(s)	355
AloI	GAACNNNNNNTCC	2 cut(s)	335, 367
AluBI	AGCT	3 cut(s)	113, 394, 439
AluI	AGCT	3 cut(s)	113, 394, 439
Alw26I	GTCTC	1 cut(s)	212
AoxI	GGCC	5 cut(s)	94, 191, 258, 447, 456
ApeKI	GCWGC	1 cut(s)	394
ApoI	RAATTY	1 cut(s)	308
Asp700I	GAANNNNTTC	1 cut(s)	212
AsuC2I	CCSGG	1 cut(s)	62
AsuHPI	GGTGA	1 cut(s)	115
BalI	TGGCCA	1 cut(s)	260
BbvCI	CCTCAGC	1 cut(s)	109
BbvI	GCAGC	1 cut(s)	381
BccI	CCATC	1 cut(s)	362
BciT130I	CCWGG	1 cut(s)	357
BciVI	GTATCC	1 cut(s)	353
BcnI	CCSGG	1 cut(s)	62
BcoDI	GTCTC	1 cut(s)	212
BfmI	CTRYAG	2 cut(s)	36, 540
BfuAI	ACCTGC	1 cut(s)	175
BfuI	GTATCC	1 cut(s)	353
BglI	GCCNNNNNGGC	2 cut(s)	62, 455
BisI	GCNGC	2 cut(s)	57, 395
BlsI	GCNGC	2 cut(s)	58, 396
Bme1390I	CCNGG	2 cut(s)	62, 357
BmiI	GGNNCC	1 cut(s)	297
BmrFI	CCNGG	2 cut(s)	62, 357
BmrI	ACTGGG	1 cut(s)	301
BmsI	GCATC	2 cut(s)	398, 525
BmuI	ACTGGG	1 cut(s)	301
BplI	GAGNNNNNCTC	2 cut(s)	209, 241
BpmI	CTGGAG	1 cut(s)	461
Bpu10I	CCTNAGC	1 cut(s)	109
BpuEI	CTTGAG	2 cut(s)	51, 83
BpuMI	CCSGG	1 cut(s)	62
BsaI	GGTCTC	1 cut(s)	212
BsaJI	CCNNGG	4 cut(s)	60, 255, 261, 355
BsaXI	ACNNNNNCTCC	2 cut(s)	156, 186
Bsc4I	CCNNNNNNNGG	3 cut(s)	92, 200, 404
Bse1I	ACTGG	3 cut(s)	97, 296, 383
Bse3DI	GCAATG	2 cut(s)	166, 182
BseBI	CCWGG	1 cut(s)	357
BseDI	CCNNGG	4 cut(s)	60, 255, 261, 355
BseGI	GGATG	2 cut(s)	413, 474
BseLI	CCNNNNNNNGG	3 cut(s)	92, 200, 404
BseMI	GCAATG	2 cut(s)	166, 182
BseMII	CTCAG	2 cut(s)	123, 437
BseNI	ACTGG	3 cut(s)	97, 296, 383
BseRI	GAGGAG	3 cut(s)	228, 269, 458
BseXI	GCAGC	1 cut(s)	381
BsgI	GTGCAG	1 cut(s)	530
Bsh1285I	CGRYCG	1 cut(s)	52
BshFI	GGCC	5 cut(s)	96, 193, 260, 449, 458
BsiEI	CGRYCG	1 cut(s)	52
BsiSI	CCGG	1 cut(s)	62
BslI	CCNNNNNNNGG	3 cut(s)	92, 200, 404
BsmAI	GTCTC	1 cut(s)	212
BsnI	GGCC	5 cut(s)	96, 193, 260, 449, 458
Bso31I	GGTCTC	1 cut(s)	212
BspACI	CCGC	4 cut(s)	24, 57, 283, 502
BspANI	GGCC	5 cut(s)	96, 193, 260, 449, 458
BspCNI	CTCAG	2 cut(s)	122, 436
BspLI	GGNNCC	1 cut(s)	297
BspMAI	CTGCAG	1 cut(s)	544
BspMI	ACCTGC	1 cut(s)	175
BspTNI	GGTCTC	1 cut(s)	212
BsrDI	GCAATG	2 cut(s)	166, 182
BsrI	ACTGG	3 cut(s)	97, 296, 383
BssECI	CCNNGG	4 cut(s)	60, 255, 261, 355
BssT1I	CCWWGG	2 cut(s)	255, 261
Bst2UI	CCWGG	1 cut(s)	357
Bst4CI	ACNGT	4 cut(s)	31, 40, 53, 338
Bst6I	CTCTTC	3 cut(s)	163, 250, 424
BstAPI	GCANNNNNTGC	1 cut(s)	181
BstC8I	GCNNGC	4 cut(s)	73, 157, 540, 544
BstDEI	CTNAG	2 cut(s)	109, 423
BstF5I	GGATG	2 cut(s)	413, 474
BstMAI	GTCTC	1 cut(s)	212
BstMCI	CGRYCG	1 cut(s)	52
BstMWI	GCNNNNNNNGC	5 cut(s)	53, 62, 181, 190, 455
BstNI	CCWGG	1 cut(s)	357
BstSCI	CCNGG	2 cut(s)	60, 355
BstSFI	CTRYAG	2 cut(s)	36, 540
BstV1I	GCAGC	1 cut(s)	381
BsuI	GTATCC	1 cut(s)	353
BsuRI	GGCC	5 cut(s)	96, 193, 260, 449, 458
BtgZI	GCGATG	1 cut(s)	511
BtsCI	GGATG	2 cut(s)	413, 474
BtsI	GCAGTG	1 cut(s)	518
BtsIMutI	CAGTG	2 cut(s)	90, 518
BveI	ACCTGC	1 cut(s)	175
Cac8I	GCNNGC	4 cut(s)	73, 157, 540, 544
Csp6I	GTAC	2 cut(s)	334, 376
CspCI	CAANNNNNGTGG	2 cut(s)	186, 221
CviAII	CATG	4 cut(s)	13, 76, 323, 400
CviQI	GTAC	2 cut(s)	334, 376
DdeI	CTNAG	2 cut(s)	109, 423
EaeI	YGGCCR	1 cut(s)	258
Eam1104I	CTCTTC	3 cut(s)	163, 250, 424
EarI	CTCTTC	3 cut(s)	163, 250, 424
EciI	GGCGGA	1 cut(s)	491
Eco130I	CCWWGG	2 cut(s)	255, 261
Eco147I	AGGCCT	1 cut(s)	458
Eco31I	GGTCTC	1 cut(s)	212
EcoRII	CCWGG	1 cut(s)	355
EcoT14I	CCWWGG	2 cut(s)	255, 261
ErhI	CCWWGG	2 cut(s)	255, 261
FaeI	CATG	4 cut(s)	16, 79, 326, 403
FaiI	YATR	5 cut(s)	14, 77, 182, 324, 401
FatI	CATG	4 cut(s)	12, 75, 322, 399
FblI	GTMKAC	1 cut(s)	35
Fnu4HI	GCNGC	2 cut(s)	57, 395
FokI	GGATG	2 cut(s)	420, 461
Fsp4HI	GCNGC	2 cut(s)	57, 395
GluI	GCNGC	2 cut(s)	57, 395
GsuI	CTGGAG	1 cut(s)	461
HaeIII	GGCC	5 cut(s)	96, 193, 260, 449, 458
HapII	CCGG	1 cut(s)	62
Hin1II	CATG	4 cut(s)	16, 79, 326, 403
HinfI	GANTC	3 cut(s)	251, 416, 489
HpaII	CCGG	1 cut(s)	62
HphI	GGTGA	1 cut(s)	115
Hpy166II	GTNNAC	3 cut(s)	36, 43, 334
Hpy188I	TCNGA	3 cut(s)	250, 407, 488
Hpy188III	TCNNGA	1 cut(s)	217
Hpy8I	GTNNAC	3 cut(s)	36, 43, 334
HpyAV	CCTTC	5 cut(s)	242, 366, 427, 448, 481
HpyCH4III	ACNGT	4 cut(s)	31, 40, 53, 338
HpyCH4IV	ACGT	1 cut(s)	498
HpyCH4V	TGCA	7 cut(s)	155, 175, 184, 411, 511, 516, 542
HpyF10VI	GCNNNNNNNGC	5 cut(s)	53, 62, 181, 190, 455
HpyF3I	CTNAG	2 cut(s)	109, 423
HpySE526I	ACGT	1 cut(s)	498
Hsp92II	CATG	4 cut(s)	16, 79, 326, 403
LmnI	GCTCC	3 cut(s)	88, 319, 436
Lsp1109I	GCAGC	1 cut(s)	381
LweI	GCATC	2 cut(s)	398, 525
MaeII	ACGT	1 cut(s)	498
MaeIII	GTNAC	1 cut(s)	121
MboII	GAAGA	4 cut(s)	180, 220, 237, 411
MlsI	TGGCCA	1 cut(s)	260
MluCI	AATT	1 cut(s)	308
MluNI	TGGCCA	1 cut(s)	260
MlyI	GAGTC	2 cut(s)	260, 410
MmeI	TCCRAC	1 cut(s)	297
Mox20I	TGGCCA	1 cut(s)	260
MroXI	GAANNNNTTC	1 cut(s)	212
MscI	TGGCCA	1 cut(s)	260
Msp20I	TGGCCA	1 cut(s)	260
MspI	CCGG	1 cut(s)	62
MspR9I	CCNGG	2 cut(s)	62, 357
MvaI	CCWGG	1 cut(s)	357
MwoI	GCNNNNNNNGC	5 cut(s)	53, 62, 181, 190, 455
NciI	CCSGG	1 cut(s)	62
NlaIII	CATG	4 cut(s)	16, 79, 326, 403
NlaIV	GGNNCC	1 cut(s)	297
NmuCI	GTSAC	1 cut(s)	121
PaqCI	CACCTGC	1 cut(s)	175
PceI	AGGCCT	1 cut(s)	458
PcsI	WCGNNNNNNNCGW	1 cut(s)	411
PdmI	GAANNNNTTC	1 cut(s)	212
PfeI	GAWTC	1 cut(s)	489
PflFI	GACNNNGTC	1 cut(s)	497
PflMI	CCANNNNNTGG	1 cut(s)	92
PkrI	GCNGC	2 cut(s)	58, 396
PleI	GAGTC	2 cut(s)	259, 410
PpsI	GAGTC	2 cut(s)	259, 410
Psp6I	CCWGG	1 cut(s)	355
PspGI	CCWGG	1 cut(s)	355
PspN4I	GGNNCC	1 cut(s)	297
PstI	CTGCAG	1 cut(s)	544
PsyI	GACNNNGTC	1 cut(s)	497
RsaI	GTAC	2 cut(s)	335, 377
RsaNI	GTAC	2 cut(s)	334, 376
SatI	GCNGC	2 cut(s)	57, 395
SbfI	CCTGCAGG	1 cut(s)	544
SchI	GAGTC	2 cut(s)	260, 410
ScrFI	CCNGG	2 cut(s)	62, 357
SdaI	CCTGCAGG	1 cut(s)	544
SfaNI	GCATC	2 cut(s)	398, 525
SfcI	CTRYAG	2 cut(s)	36, 540
SfiI	GGCCNNNNNGGCC	1 cut(s)	455
SmlI	CTYRAG	2 cut(s)	66, 98
SmoI	CTYRAG	2 cut(s)	66, 98
Sse8387I	CCTGCAGG	1 cut(s)	544
Sse9I	AATT	1 cut(s)	308
SseBI	AGGCCT	1 cut(s)	458
SsiI	CCGC	4 cut(s)	24, 57, 283, 502
StuI	AGGCCT	1 cut(s)	458
StyD4I	CCNGG	2 cut(s)	60, 355
StyI	CCWWGG	2 cut(s)	255, 261
TaaI	ACNGT	4 cut(s)	31, 40, 53, 338
TaiI	ACGT	1 cut(s)	501
TaqI	TCGA	2 cut(s)	216, 492
TasI	AATT	1 cut(s)	308
TauI	GCSGC	1 cut(s)	59
TfiI	GAWTC	1 cut(s)	489
TscAI	CASTG	2 cut(s)	97, 518
TseFI	GTSAC	1 cut(s)	121
TseI	GCWGC	1 cut(s)	394
Tsp45I	GTSAC	1 cut(s)	121
TspDTI	ATGAA	2 cut(s)	463, 514
TspGWI	ACGGA	1 cut(s)	467
TspRI	CASTG	2 cut(s)	97, 518
Tth111I	GACNNNGTC	1 cut(s)	497
Van91I	CCANNNNNTGG	1 cut(s)	92
XapI	RAATTY	1 cut(s)	308
XmiI	GTMKAC	1 cut(s)	35
XmnI	GAANNNNTTC	1 cut(s)	212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.