Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002444

Physical Location & Seq

Genomic Coordinates Forward (+)

29290761 .. 29292229

Gene Structure 1469 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1244400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 540 bp

ATGGCTTCCTCCATGATTTCCTCCGGTACCGTGGCTACAGTTTCCGCCCCCGCTCAAGCCAGAATGGTTGCTCCATTCACCGGCCTCAAGTCCTCCTCAGCTTTCCCTGTCACCAGAAAAAGCAATGACATTACCTCTATTGCAAGCAATGGAGGAAGAGTGCAATGCATGCAGGTGTGGCCTCCACTTGGACTGAAGAAGTTCGAGACCCTCTCTTACCTTCCTCCCCTTTCTACCGAGTCCTTGGCCAAGGAAGTTGACTACCTCCTCCGCAAAAACTGGGTTCCCTGCTTGGAATTTGAGTTGGAGCATGGATTCGTGTACCGTGAGAACCACAGATCCCCAGGATACTATGATGGAAGGTACTGGACAATGTGGAAGCTGCCCATGTTCGGATGCACCGACTCTTCCCAGGTGTTGAAAGAGCTGGAAGAGGCCAAGAAGGCCTACCCCCAATCCTTCATCCGTATCATCGGATTCGACAATGTCCGTCAAGTGCAGTGCATCAGTTTCATCGCTTACAAGCCTCCAGGCGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00630 ko00710 ko01100 ko01120 ko01200 map00630 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

RbcS RuBisCO_small

Protein Analysis

180

Amino Acids

20.1

Weight (kDa)

9.05

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RbcS	PF12338	2 - 42	1.4e-19	Ribulose-1,5-bisphosphate carboxylase small subunit
RuBisCO_small	PF00101	68 - 176	3e-37	Ribulose bisphosphate carboxylase, small chain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002444)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67090 AT1G67090 AT5G38410 AT5G38410 AT5G38410 AT5G38420 AT5G38430 AT5G38430
fragaria_vesca	FvH4_4g27330 FvH4_6g26030
malus_domestica	MD09G1252100.v1.1 MD16G1042700.v1.1 MD17G1244400.v1.1
prunus_persica	Prupe.1G311400_v2.0.a1 Prupe.3G157200_v2.0.a1 Prupe.3G157200_v2.0.a1
pyrus_communis	pycom09g16870 pycom09g16880 pycom16g03670 pycom17g24720
rosa_chinensis	RchiOBHm_Chr2g0126111 RchiOBHm_Chr4g0435151
rosa_laevigata	RLG00000006615 RLG00000018888
rosa_multiflora	Rmu_co8187892.1_g000001 Rmu_sc0012196.1_g000003 Rmu_ssc0000339.1_g000037
rosa_roxburghii	Rroxscaffold_2G00117430 Rroxscaffold_5G00376370
rosa_rugosa	Rorug02G0261500 Rorug04G0282600
rosa_samantha	Rh2AG318400 Rh2BG326700 Rh2DG344900 Rh2DG346000 Rh4AG338200 Rh4BG346300 Rh4CG360800 Rh4DG340700
rosa_wichuraiana	Rw2G025780 Rw4G029500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	163
Acc36I	ACCTGC	1 cut(s)	163
Acc65I	GGTACC	1 cut(s)	26
AccB1I	GGYRCC	1 cut(s)	26
AccBSI	CCGCTC	1 cut(s)	53
AciI	CCGC	3 cut(s)	45, 51, 271
AclWI	GGATC	1 cut(s)	333
AcoI	YGGCCR	1 cut(s)	246
AcsI	RAATTY	1 cut(s)	296
AcuI	CTGAAG	1 cut(s)	215
AcyI	GRCGYC	1 cut(s)	534
AfaI	GTAC	3 cut(s)	28, 323, 365
AfiI	CCNNNNNNNGG	3 cut(s)	80, 188, 392
AgsI	TTSAA	1 cut(s)	421
AjnI	CCWGG	3 cut(s)	343, 411, 529
AloI	GAACNNNNNNTCC	2 cut(s)	323, 355
AluBI	AGCT	3 cut(s)	101, 382, 427
AluI	AGCT	3 cut(s)	101, 382, 427
Alw26I	GTCTC	1 cut(s)	200
AlwI	GGATC	1 cut(s)	333
AoxI	GGCC	5 cut(s)	82, 179, 246, 435, 444
ApeKI	GCWGC	1 cut(s)	382
ApoI	RAATTY	1 cut(s)	296
Asp700I	GAANNNNTTC	1 cut(s)	200
Asp718I	GGTACC	1 cut(s)	26
AsuHPI	GGTGA	2 cut(s)	70, 103
BalI	TGGCCA	1 cut(s)	248
BanI	GGYRCC	1 cut(s)	26
BbvCI	CCTCAGC	1 cut(s)	97
BbvI	GCAGC	1 cut(s)	369
BccI	CCATC	1 cut(s)	350
BciT130I	CCWGG	3 cut(s)	345, 413, 531
BciVI	GTATCC	1 cut(s)	341
BcoDI	GTCTC	1 cut(s)	200
BfmI	CTRYAG	1 cut(s)	36
BfuAI	ACCTGC	1 cut(s)	163
BfuI	GTATCC	1 cut(s)	341
BglI	GCCNNNNNGGC	1 cut(s)	443
BisI	GCNGC	1 cut(s)	383
BlsI	GCNGC	1 cut(s)	384
Bme1390I	CCNGG	3 cut(s)	345, 413, 531
BmiI	GGNNCC	2 cut(s)	28, 285
BmrFI	CCNGG	3 cut(s)	345, 413, 531
BmrI	ACTGGG	1 cut(s)	289
BmsI	GCATC	2 cut(s)	386, 513
BmuI	ACTGGG	1 cut(s)	289
BplI	GAGNNNNNCTC	2 cut(s)	197, 229
BpmI	CTGGAG	1 cut(s)	513
Bpu10I	CCTNAGC	1 cut(s)	97
BpuEI	CTTGAG	2 cut(s)	39, 71
BsaHI	GRCGYC	1 cut(s)	534
BsaI	GGTCTC	1 cut(s)	200
BsaJI	CCNNGG	5 cut(s)	30, 243, 249, 343, 411
BsaWI	WCCGGW	1 cut(s)	23
BsaXI	ACNNNNNCTCC	2 cut(s)	144, 174
Bsc4I	CCNNNNNNNGG	3 cut(s)	80, 188, 392
Bse118I	RCCGGY	1 cut(s)	80
Bse1I	ACTGG	2 cut(s)	284, 371
Bse3DI	GCAATG	3 cut(s)	130, 154, 170
BseBI	CCWGG	3 cut(s)	345, 413, 531
BseDI	CCNNGG	5 cut(s)	30, 243, 249, 343, 411
BseGI	GGATG	2 cut(s)	401, 462
BseLI	CCNNNNNNNGG	3 cut(s)	80, 188, 392
BseMI	GCAATG	3 cut(s)	130, 154, 170
BseMII	CTCAG	1 cut(s)	111
BseNI	ACTGG	2 cut(s)	284, 371
BseRI	GAGGAG	2 cut(s)	85, 257
BseXI	GCAGC	1 cut(s)	369
BsgI	GTGCAG	1 cut(s)	518
BshFI	GGCC	5 cut(s)	84, 181, 248, 437, 446
BshNI	GGYRCC	1 cut(s)	26
BsiSI	CCGG	2 cut(s)	24, 81
BslI	CCNNNNNNNGG	3 cut(s)	80, 188, 392
BsmAI	GTCTC	1 cut(s)	200
BsnI	GGCC	5 cut(s)	84, 181, 248, 437, 446
Bso31I	GGTCTC	1 cut(s)	200
Bsp143I	GATC	1 cut(s)	338
BspACI	CCGC	3 cut(s)	45, 51, 271
BspANI	GGCC	5 cut(s)	84, 181, 248, 437, 446
BspCNI	CTCAG	1 cut(s)	110
BspLI	GGNNCC	2 cut(s)	28, 285
BspMI	ACCTGC	1 cut(s)	163
BspPI	GGATC	1 cut(s)	333
BspT107I	GGYRCC	1 cut(s)	26
BspTNI	GGTCTC	1 cut(s)	200
BsrBI	CCGCTC	1 cut(s)	53
BsrDI	GCAATG	3 cut(s)	130, 154, 170
BsrFI	RCCGGY	1 cut(s)	80
BsrI	ACTGG	2 cut(s)	284, 371
BssAI	RCCGGY	1 cut(s)	80
BssECI	CCNNGG	5 cut(s)	30, 243, 249, 343, 411
BssMI	GATC	1 cut(s)	338
BssNI	GRCGYC	1 cut(s)	534
BssT1I	CCWWGG	2 cut(s)	243, 249
Bst2UI	CCWGG	3 cut(s)	345, 413, 531
Bst4CI	ACNGT	3 cut(s)	31, 40, 326
Bst6I	CTCTTC	3 cut(s)	151, 412, 426
BstACI	GRCGYC	1 cut(s)	534
BstAPI	GCANNNNNTGC	1 cut(s)	169
BstC8I	GCNNGC	2 cut(s)	145, 170
BstDEI	CTNAG	1 cut(s)	97
BstDSI	CCRYGG	1 cut(s)	30
BstF5I	GGATG	2 cut(s)	401, 462
BstKTI	GATC	1 cut(s)	341
BstMAI	GTCTC	1 cut(s)	200
BstMBI	GATC	1 cut(s)	338
BstMWI	GCNNNNNNNGC	3 cut(s)	169, 178, 443
BstNI	CCWGG	3 cut(s)	345, 413, 531
BstNSI	RCATGY	1 cut(s)	172
BstSCI	CCNGG	3 cut(s)	343, 411, 529
BstSFI	CTRYAG	1 cut(s)	36
BstV1I	GCAGC	1 cut(s)	369
BstX2I	RGATCY	1 cut(s)	338
BstYI	RGATCY	1 cut(s)	338
BsuI	GTATCC	1 cut(s)	341
BsuRI	GGCC	5 cut(s)	84, 181, 248, 437, 446
BtgI	CCRYGG	1 cut(s)	30
BtgZI	GCGATG	1 cut(s)	499
BtsCI	GGATG	2 cut(s)	401, 462
BtsI	GCAGTG	1 cut(s)	506
BtsIMutI	CAGTG	1 cut(s)	506
BveI	ACCTGC	1 cut(s)	163
Cac8I	GCNNGC	2 cut(s)	145, 170
Cfr10I	RCCGGY	1 cut(s)	80
CseI	GACGC	1 cut(s)	523
Csp6I	GTAC	3 cut(s)	27, 322, 364
CviAII	CATG	4 cut(s)	13, 169, 311, 388
CviQI	GTAC	3 cut(s)	27, 322, 364
DdeI	CTNAG	1 cut(s)	97
DpnI	GATC	1 cut(s)	340
DpnII	GATC	1 cut(s)	338
EaeI	YGGCCR	1 cut(s)	246
Eam1104I	CTCTTC	3 cut(s)	151, 412, 426
EarI	CTCTTC	3 cut(s)	151, 412, 426
EciI	GGCGGA	1 cut(s)	34
Eco130I	CCWWGG	2 cut(s)	243, 249
Eco147I	AGGCCT	1 cut(s)	446
Eco31I	GGTCTC	1 cut(s)	200
Eco57I	CTGAAG	1 cut(s)	215
EcoRII	CCWGG	3 cut(s)	343, 411, 529
EcoT14I	CCWWGG	2 cut(s)	243, 249
EcoT22I	ATGCAT	1 cut(s)	170
ErhI	CCWWGG	2 cut(s)	243, 249
FaeI	CATG	4 cut(s)	16, 172, 314, 391
FaiI	YATR	5 cut(s)	14, 170, 312, 354, 389
FatI	CATG	4 cut(s)	12, 168, 310, 387
FauI	CCCGC	1 cut(s)	58
Fnu4HI	GCNGC	1 cut(s)	383
FokI	GGATG	2 cut(s)	408, 449
Fsp4HI	GCNGC	1 cut(s)	383
GluI	GCNGC	1 cut(s)	383
GsuI	CTGGAG	1 cut(s)	513
HaeIII	GGCC	5 cut(s)	84, 181, 248, 437, 446
HapII	CCGG	2 cut(s)	24, 81
HgaI	GACGC	1 cut(s)	523
Hin1I	GRCGYC	1 cut(s)	534
Hin1II	CATG	4 cut(s)	16, 172, 314, 391
HincII	GTYRAC	1 cut(s)	259
HindII	GTYRAC	1 cut(s)	259
HinfI	GANTC	4 cut(s)	239, 315, 404, 477
HpaII	CCGG	2 cut(s)	24, 81
HphI	GGTGA	2 cut(s)	70, 103
Hpy166II	GTNNAC	2 cut(s)	259, 322
Hpy188I	TCNGA	2 cut(s)	395, 476
Hpy188III	TCNNGA	1 cut(s)	205
Hpy8I	GTNNAC	2 cut(s)	259, 322
HpyAV	CCTTC	4 cut(s)	230, 354, 436, 469
HpyCH4III	ACNGT	3 cut(s)	31, 40, 326
HpyCH4V	TGCA	7 cut(s)	143, 163, 168, 172, 399, 499, 504
HpyF10VI	GCNNNNNNNGC	3 cut(s)	169, 178, 443
HpyF3I	CTNAG	1 cut(s)	97
Hsp92I	GRCGYC	1 cut(s)	534
Hsp92II	CATG	4 cut(s)	16, 172, 314, 391
KpnI	GGTACC	1 cut(s)	30
Kzo9I	GATC	1 cut(s)	338
LmnI	GCTCC	2 cut(s)	76, 307
Lsp1109I	GCAGC	1 cut(s)	369
LweI	GCATC	2 cut(s)	386, 513
MaeIII	GTNAC	1 cut(s)	109
MalI	GATC	1 cut(s)	340
MbiI	CCGCTC	1 cut(s)	53
MboI	GATC	1 cut(s)	338
MboII	GAAGA	4 cut(s)	168, 208, 399, 443
MflI	RGATCY	1 cut(s)	338
MlsI	TGGCCA	1 cut(s)	248
MluCI	AATT	1 cut(s)	296
MluNI	TGGCCA	1 cut(s)	248
MlyI	GAGTC	2 cut(s)	248, 398
MmeI	TCCRAC	1 cut(s)	285
Mox20I	TGGCCA	1 cut(s)	248
Mph1103I	ATGCAT	1 cut(s)	170
MroXI	GAANNNNTTC	1 cut(s)	200
MscI	TGGCCA	1 cut(s)	248
MslI	CAYNNNNRTG	1 cut(s)	173
Msp20I	TGGCCA	1 cut(s)	248
MspI	CCGG	2 cut(s)	24, 81
MspR9I	CCNGG	3 cut(s)	345, 413, 531
MvaI	CCWGG	3 cut(s)	345, 413, 531
MwoI	GCNNNNNNNGC	3 cut(s)	169, 178, 443
NdeII	GATC	1 cut(s)	338
NlaIII	CATG	4 cut(s)	16, 172, 314, 391
NlaIV	GGNNCC	2 cut(s)	28, 285
NmuCI	GTSAC	1 cut(s)	109
NsiI	ATGCAT	1 cut(s)	170
NspI	RCATGY	1 cut(s)	172
PaeI	GCATGC	1 cut(s)	172
PaqCI	CACCTGC	1 cut(s)	163
PceI	AGGCCT	1 cut(s)	446
PcsI	WCGNNNNNNNCGW	1 cut(s)	399
PdmI	GAANNNNTTC	1 cut(s)	200
PfeI	GAWTC	2 cut(s)	315, 477
PflFI	GACNNNGTC	1 cut(s)	485
PkrI	GCNGC	1 cut(s)	384
PleI	GAGTC	2 cut(s)	247, 398
PpsI	GAGTC	2 cut(s)	247, 398
Psp6I	CCWGG	3 cut(s)	343, 411, 529
PspGI	CCWGG	3 cut(s)	343, 411, 529
PspN4I	GGNNCC	2 cut(s)	28, 285
PsuI	RGATCY	1 cut(s)	338
PsyI	GACNNNGTC	1 cut(s)	485
RsaI	GTAC	3 cut(s)	28, 323, 365
RsaNI	GTAC	3 cut(s)	27, 322, 364
RseI	CAYNNNNRTG	1 cut(s)	173
SatI	GCNGC	1 cut(s)	383
Sau3AI	GATC	1 cut(s)	338
SchI	GAGTC	2 cut(s)	248, 398
ScrFI	CCNGG	3 cut(s)	345, 413, 531
SetI	ASST	9 cut(s)	103, 137, 177, 222, 267, 365, 384, 417, 429
SfaNI	GCATC	2 cut(s)	386, 513
SfcI	CTRYAG	1 cut(s)	36
SfiI	GGCCNNNNNGGCC	1 cut(s)	443
SmiMI	CAYNNNNRTG	1 cut(s)	173
SmlI	CTYRAG	2 cut(s)	54, 86
SmoI	CTYRAG	2 cut(s)	54, 86
SphI	GCATGC	1 cut(s)	172
Sse9I	AATT	1 cut(s)	296
SseBI	AGGCCT	1 cut(s)	446
SsiI	CCGC	3 cut(s)	45, 51, 271
StuI	AGGCCT	1 cut(s)	446
StyD4I	CCNGG	3 cut(s)	343, 411, 529
StyI	CCWWGG	2 cut(s)	243, 249
TaaI	ACNGT	3 cut(s)	31, 40, 326
TaqI	TCGA	2 cut(s)	204, 480
TasI	AATT	1 cut(s)	296
TfiI	GAWTC	2 cut(s)	315, 477
TscAI	CASTG	1 cut(s)	506
TseFI	GTSAC	1 cut(s)	109
TseI	GCWGC	1 cut(s)	382
Tsp45I	GTSAC	1 cut(s)	109
TspDTI	ATGAA	2 cut(s)	451, 502
TspGWI	ACGGA	2 cut(s)	455, 479
TspRI	CASTG	1 cut(s)	506
Tth111I	GACNNNGTC	1 cut(s)	485
XapI	RAATTY	1 cut(s)	296
XceI	RCATGY	1 cut(s)	172
XmnI	GAANNNNTTC	1 cut(s)	200
Zsp2I	ATGCAT	1 cut(s)	170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.