MD13G1046900.v1.1

Leucoanthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016318
Physical Location & Seq
Forward (+)
3255682 .. 3260014
4333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1046900.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGGCAAAAATAGTAACAAAATCGAAGTATCCTCCTATATATAGACAACATATATTCCTCCCTTACTTCACACACTTCGCATGCCACAATCGTGTCAAAAATCACTTGGGAAAAAAGGACTTTTCAAACTTGAGGGCAAAAAAAAAAAAAAACATACAAAGAAGAAGAAAAGTCAAGGCAGTCTGTAGCAGGAAGGAAGAGCTAGCAGCAGCAGCAGCCATGACTGTTTCACCTTCTCTTTCTGTTGCCAGAAATGGCAGAGTCCTCATTGTCGGTGCCACCGGTTTCATCGGGAGGTTTGTAGCTGAAGCCAGCCTCGCCGCCGGCCGACCCACGTATGTTCTCGTCCGGCCCGGCCCTCTTCATCCTTCTAAGGCTGACACCGTCAAGTCCTTCAAACACAAAGGCGCCATAATCTTACACGGGCTGATTTCTGATAAAACCCTAATGGAGAAGATACTGAGAGAGCATGAAATCGAGACAGTAATATCAGCCGTCGGTGGTGCGACAATATTGGACCAGATTGCCTTAGTCGAGGCCATTGCAGCAGTTGGTACAGTTAAGAGGTTTTTGCCCTCCGAGTTTGGACATGATGTGGACAGGGCTGATCCGGTTGAACCAGGGCTCACCATGTACGAGGAGAAACGCAAGGTTAGACGTTGGGTCGAGAAATCTGGGGTGGCCTACACATACATTTGCTGCAACTCCATTGCCTCTTGGCCCTACTTTGACAACACACACCCTTCTGAGGTTCTCCCACCGTTGGATCGGTTCCAAATCTATGGTGATGGCACCGTCAAAGCCTATTTTGTAGACGGTACGGATATTGGAAAATTCACAATAAAGACCGTGGACGACATCCGAACAATCAACAAAAATATTCATTTCCGACCGCCGAGCAATTTATACGACATCAATGGGCTTGCATCTCTGTGGGAGAAGAAAATTGGCCGGACTCTCCCTAGAATCACCATCACTGAAAATCACTTACTAGCCCTTGCTGCAGAGAATCGTATACCAGAAAGTATAGTGGCATCGTTCACGCATGACATCTTTATCAAAGGATGTCAGGTGAATTTTGCAGTGGAGGGGCCTCGTGATGTGGAAGTGGGAACTCTCTACCCCGGAGATTCTTTCAGGACCTTGGATGAGTGCTTCAATGACTTTCTTCTCAAGTTGAAGGACAACCTAGAGCCAGTGCATGAGGAGAACGTCTCCACCAAAAACGCCGTCGTCGAATCCAGGGCTGTCACTCCCACTTGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
NmrA

Protein Analysis

423

Amino Acids

47.08

Weight (kDa)

9.34

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 86 - 384 1.1e-52 NmrA-like family
Epimerase PF01370 88 - 173 3.7e-06 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 92 - 238 1.4e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016318)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g28110
malus_domestica MD13G1046900.v1.1 MD16G1048500.v1.1
prunus_persica Prupe.1G305100_v2.0.a1
pyrus_communis pycom13g04100 pycom16g04240
rosa_chinensis RchiOBHm_Chr4g0435881
rosa_laevigata RLG00000006553
rosa_multiflora Rmu_sc0003165.1_g000010
rosa_roxburghii Rroxscaffold_5G00377050
rosa_rugosa Rorug04G0290200
rosa_samantha Rh4BG353500 Rh4CG367900 Rh4DG347300
rosa_wichuraiana Rw4G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 275, 407, 791
AccI GTMKAC 2 cut(s) 813, 1015
AciI CCGC 2 cut(s) 321, 893
AclWI GGATC 2 cut(s) 602, 774
AcoI YGGCCR 2 cut(s) 325, 949
AcsI RAATTY 2 cut(s) 833, 1075
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 408
AfaI GTAC 3 cut(s) 556, 635, 820
AfiI CCNNNNNNNGG 2 cut(s) 748, 763
AgeI ACCGGT 1 cut(s) 281
AgsI TTSAA 5 cut(s) 126, 397, 617, 1159, 1180
AhdI GACNNNNNGTC 1 cut(s) 662
AjnI CCWGG 2 cut(s) 619, 1241
AleI CACNNNNGTG 1 cut(s) 90
AluBI AGCT 2 cut(s) 202, 305
AluI AGCT 2 cut(s) 202, 305
Alw26I GTCTC 2 cut(s) 473, 1219
AlwI GGATC 2 cut(s) 602, 774
AoxI GGCC 8 cut(s) 325, 350, 355, 537, 681, 719, 949, 1091
ApeKI GCWGC 7 cut(s) 206, 209, 212, 215, 545, 699, 1001
ApoI RAATTY 2 cut(s) 833, 1075
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
AsiGI ACCGGT 1 cut(s) 281
AspLEI GCGC 1 cut(s) 410
AspS9I GGNCC 6 cut(s) 351, 356, 517, 720, 1091, 1140
AsuC2I CCSGG 2 cut(s) 354, 1125
AsuHPI GGTGA 5 cut(s) 222, 619, 797, 961, 1084
AsuNHI GCTAGC 1 cut(s) 202
AvaII GGWCC 2 cut(s) 517, 1140
BanI GGYRCC 3 cut(s) 275, 407, 791
BanII GRGCYC 1 cut(s) 627
BauI CACGAG 1 cut(s) 1095
BbvI GCAGC 7 cut(s) 218, 221, 224, 227, 557, 686, 988
BccI CCATC 2 cut(s) 782, 980
BceAI ACGGC 2 cut(s) 479, 1214
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 2 cut(s) 621, 1243
BciVI GTATCC 1 cut(s) 39
BcnI CCSGG 2 cut(s) 354, 1125
BcoDI GTCTC 2 cut(s) 473, 1219
BfaI CTAG 4 cut(s) 203, 963, 992, 1190
BfmI CTRYAG 2 cut(s) 184, 1002
BfoI RGCGCY 1 cut(s) 411
BfuI GTATCC 1 cut(s) 39
BisI GCNGC 8 cut(s) 207, 210, 213, 216, 321, 546, 700, 1002
BlsI GCNGC 8 cut(s) 208, 211, 214, 217, 322, 547, 701, 1003
Bme1390I CCNGG 4 cut(s) 354, 621, 1125, 1243
Bme18I GGWCC 2 cut(s) 517, 1140
BmeRI GACNNNNNGTC 1 cut(s) 662
BmgT120I GGNCC 6 cut(s) 351, 356, 517, 720, 1091, 1140
BmiI GGNNCC 5 cut(s) 277, 409, 773, 793, 1092
BmrFI CCNGG 4 cut(s) 354, 621, 1125, 1243
BmsI GCATC 2 cut(s) 935, 1043
BmtI GCTAGC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 1199, 1231
BpuEI CTTGAG 2 cut(s) 151, 1157
BpuMI CCSGG 2 cut(s) 354, 1125
BsaAI YACGTR 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 408
BsaJI CCNNGG 5 cut(s) 620, 849, 1123, 1143, 1242
BsaWI WCCGGW 2 cut(s) 281, 610
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 763
Bse118I RCCGGY 2 cut(s) 281, 323
Bse1I ACTGG 1 cut(s) 1196
Bse3DI GCAATG 2 cut(s) 540, 708
BseBI CCWGG 2 cut(s) 621, 1243
BseDI CCNNGG 5 cut(s) 620, 849, 1123, 1143, 1242
BseGI GGATG 4 cut(s) 364, 858, 1070, 1153
BseLI CCNNNNNNNGG 2 cut(s) 748, 763
BseMI GCAATG 2 cut(s) 540, 708
BseMII CTCAG 2 cut(s) 452, 738
BseNI ACTGG 1 cut(s) 1196
BseRI GAGGAG 2 cut(s) 653, 1220
BseX3I CGGCCG 1 cut(s) 325
BseXI GCAGC 7 cut(s) 218, 221, 224, 227, 557, 686, 988
Bsh1285I CGRYCG 2 cut(s) 328, 893
BshFI GGCC 8 cut(s) 327, 352, 357, 539, 683, 721, 951, 1093
BshNI GGYRCC 3 cut(s) 275, 407, 791
BshTI ACCGGT 1 cut(s) 281
BsiEI CGRYCG 2 cut(s) 328, 893
BsiSI CCGG 7 cut(s) 282, 324, 349, 354, 611, 952, 1125
BslI CCNNNNNNNGG 2 cut(s) 748, 763
BsmAI GTCTC 2 cut(s) 473, 1219
BsmBI CGTCTC 1 cut(s) 1219
BsnI GGCC 8 cut(s) 327, 352, 357, 539, 683, 721, 951, 1093
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 2 cut(s) 607, 766
BspACI CCGC 2 cut(s) 321, 893
BspANI GGCC 8 cut(s) 327, 352, 357, 539, 683, 721, 951, 1093
BspCNI CTCAG 2 cut(s) 453, 739
BspLI GGNNCC 5 cut(s) 277, 409, 773, 793, 1092
BspMAI CTGCAG 1 cut(s) 1006
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 2 cut(s) 602, 774
BspQI GCTCTTC 1 cut(s) 192
BspT107I GGYRCC 3 cut(s) 275, 407, 791
BsrDI GCAATG 2 cut(s) 540, 708
BsrFI RCCGGY 2 cut(s) 281, 323
BsrI ACTGG 1 cut(s) 1196
BssAI RCCGGY 2 cut(s) 281, 323
BssECI CCNNGG 5 cut(s) 620, 849, 1123, 1143, 1242
BssMI GATC 2 cut(s) 607, 766
BssNAI GTATAC 1 cut(s) 1016
BssNI GRCGYC 1 cut(s) 408
BssSI CACGAG 1 cut(s) 1095
BssT1I CCWWGG 1 cut(s) 1143
Bst1107I GTATAC 1 cut(s) 1016
Bst2BI CACGAG 1 cut(s) 1095
Bst2UI CCWGG 2 cut(s) 621, 1243
Bst4CI ACNGT 8 cut(s) 226, 385, 484, 559, 762, 796, 818, 850
Bst6I CTCTTC 2 cut(s) 192, 366
BstACI GRCGYC 1 cut(s) 408
BstBAI YACGTR 1 cut(s) 336
BstC8I GCNNGC 5 cut(s) 82, 204, 313, 325, 924
BstDEI CTNAG 4 cut(s) 372, 461, 529, 747
BstDSI CCRYGG 1 cut(s) 849
BstF5I GGATG 4 cut(s) 364, 858, 1070, 1153
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 1 cut(s) 410
BstKTI GATC 2 cut(s) 610, 769
BstMAI GTCTC 2 cut(s) 473, 1219
BstMBI GATC 2 cut(s) 607, 766
BstMCI CGRYCG 2 cut(s) 328, 893
BstMWI GCNNNNNNNGC 5 cut(s) 212, 215, 317, 545, 1001
BstNI CCWGG 2 cut(s) 621, 1243
BstNSI RCATGY 1 cut(s) 84
BstSCI CCNGG 4 cut(s) 352, 619, 1123, 1241
BstSFI CTRYAG 2 cut(s) 184, 1002
BstV1I GCAGC 7 cut(s) 218, 221, 224, 227, 557, 686, 988
BstXI CCANNNNNNTGG 1 cut(s) 782
BstZ17I GTATAC 1 cut(s) 1016
BstZI CGGCCG 1 cut(s) 325
BsuI GTATCC 1 cut(s) 39
BsuRI GGCC 8 cut(s) 327, 352, 357, 539, 683, 721, 951, 1093
BtgI CCRYGG 1 cut(s) 849
BtsCI GGATG 4 cut(s) 364, 858, 1070, 1153
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 3 cut(s) 975, 1089, 1203
Cac8I GCNNGC 5 cut(s) 82, 204, 313, 325, 924
CfoI GCGC 1 cut(s) 410
Cfr10I RCCGGY 2 cut(s) 281, 323
Cfr13I GGNCC 6 cut(s) 351, 356, 517, 720, 1091, 1140
Csp6I GTAC 3 cut(s) 555, 634, 819
CspAI ACCGGT 1 cut(s) 281
CviAII CATG 7 cut(s) 81, 220, 470, 590, 631, 1046, 1202
CviQI GTAC 3 cut(s) 555, 634, 819
DdeI CTNAG 4 cut(s) 372, 461, 529, 747
DinI GGCGCC 1 cut(s) 409
DpnI GATC 2 cut(s) 609, 768
DpnII GATC 2 cut(s) 607, 766
DriI GACNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 2 cut(s) 325, 949
EagI CGGCCG 1 cut(s) 325
Eam1104I CTCTTC 2 cut(s) 192, 366
Eam1105I GACNNNNNGTC 1 cut(s) 662
EarI CTCTTC 2 cut(s) 192, 366
EclXI CGGCCG 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 1143
Eco24I GRGCYC 1 cut(s) 627
Eco47I GGWCC 2 cut(s) 517, 1140
Eco52I CGGCCG 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 327
EcoO109I RGGNCCY 2 cut(s) 1091, 1140
EcoRII CCWGG 2 cut(s) 619, 1241
EcoT14I CCWWGG 1 cut(s) 1143
EcoT38I GRGCYC 1 cut(s) 627
EgeI GGCGCC 1 cut(s) 409
EheI GGCGCC 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 1143
Esp3I CGTCTC 1 cut(s) 1219
FaeI CATG 7 cut(s) 84, 223, 473, 593, 634, 1049, 1205
FatI CATG 7 cut(s) 80, 219, 469, 589, 630, 1045, 1201
FblI GTMKAC 2 cut(s) 813, 1015
Fnu4HI GCNGC 8 cut(s) 207, 210, 213, 216, 321, 546, 700, 1002
FokI GGATG 4 cut(s) 351, 845, 1077, 1160
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 8 cut(s) 207, 210, 213, 216, 321, 546, 700, 1002
FspBI CTAG 4 cut(s) 203, 963, 992, 1190
GlaI GCGC 1 cut(s) 409
GluI GCNGC 8 cut(s) 207, 210, 213, 216, 321, 546, 700, 1002
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 8 cut(s) 327, 352, 357, 539, 683, 721, 951, 1093
HapII CCGG 7 cut(s) 282, 324, 349, 354, 611, 952, 1125
HhaI GCGC 1 cut(s) 410
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 7 cut(s) 84, 223, 473, 593, 634, 1049, 1205
Hin6I GCGC 1 cut(s) 408
HinP1I GCGC 1 cut(s) 408
HinfI GANTC 6 cut(s) 261, 955, 966, 1009, 1130, 1238
HpaII CCGG 7 cut(s) 282, 324, 349, 354, 611, 952, 1125
HphI GGTGA 5 cut(s) 222, 619, 797, 961, 1084
Hpy166II GTNNAC 5 cut(s) 598, 814, 853, 1016, 1041
Hpy188I TCNGA 5 cut(s) 436, 580, 748, 863, 890
Hpy188III TCNNGA 5 cut(s) 292, 478, 667, 1097, 1138
Hpy8I GTNNAC 5 cut(s) 598, 814, 853, 1016, 1041
Hpy99I CGWCG 3 cut(s) 500, 1235, 1238
HpyAV CCTTC 6 cut(s) 187, 243, 378, 403, 753, 1174
HpyCH4III ACNGT 8 cut(s) 226, 385, 484, 559, 762, 796, 818, 850
HpyCH4IV ACGT 3 cut(s) 335, 658, 1212
HpyCH4V TGCA 6 cut(s) 545, 702, 926, 1004, 1082, 1201
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 215, 317, 545, 1001
HpyF3I CTNAG 4 cut(s) 372, 461, 529, 747
HpySE526I ACGT 3 cut(s) 335, 658, 1212
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 7 cut(s) 84, 223, 473, 593, 634, 1049, 1205
HspAI GCGC 1 cut(s) 408
KasI GGCGCC 1 cut(s) 407
KroI GCCGGC 1 cut(s) 323
KroNI GCCGGC 1 cut(s) 325
Kzo9I GATC 2 cut(s) 607, 766
LguI GCTCTTC 1 cut(s) 192
Lsp1109I GCAGC 7 cut(s) 218, 221, 224, 227, 557, 686, 988
LweI GCATC 2 cut(s) 935, 1043
MaeI CTAG 4 cut(s) 203, 963, 992, 1190
MaeII ACGT 3 cut(s) 335, 658, 1212
MaeIII GTNAC 2 cut(s) 13, 1249
MalI GATC 2 cut(s) 609, 768
MboI GATC 2 cut(s) 607, 766
MboII GAAGA 7 cut(s) 174, 177, 209, 353, 466, 952, 1160
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 4 cut(s) 833, 901, 945, 1075
Mly113I GGCGCC 1 cut(s) 408
MlyI GAGTC 2 cut(s) 270, 949
MmeI TCCRAC 2 cut(s) 744, 913
MroNI GCCGGC 1 cut(s) 323
MseI TTAA 1 cut(s) 561
MslI CAYNNNNRTG 2 cut(s) 90, 931
MspI CCGG 7 cut(s) 282, 324, 349, 354, 611, 952, 1125
MspR9I CCNGG 4 cut(s) 354, 621, 1125, 1243
MvaI CCWGG 2 cut(s) 621, 1243
MwoI GCNNNNNNNGC 5 cut(s) 212, 215, 317, 545, 1001
NaeI GCCGGC 1 cut(s) 325
NarI GGCGCC 1 cut(s) 408
NciI CCSGG 2 cut(s) 354, 1125
NdeII GATC 2 cut(s) 607, 766
NgoMIV GCCGGC 1 cut(s) 323
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 7 cut(s) 84, 223, 473, 593, 634, 1049, 1205
NlaIV GGNNCC 5 cut(s) 277, 409, 773, 793, 1092
NmeAIII GCCGAG 1 cut(s) 921
NmuCI GTSAC 1 cut(s) 1249
NspI RCATGY 1 cut(s) 84
OliI CACNNNNGTG 1 cut(s) 90
PaeI GCATGC 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 1233
PdiI GCCGGC 1 cut(s) 325
PfeI GAWTC 4 cut(s) 966, 1009, 1130, 1238
PflFI GACNNNGTC 1 cut(s) 383
PinAI ACCGGT 1 cut(s) 281
PkrI GCNGC 8 cut(s) 208, 211, 214, 217, 322, 547, 701, 1003
PleI GAGTC 2 cut(s) 269, 949
PluTI GGCGCC 1 cut(s) 411
PpsI GAGTC 2 cut(s) 269, 949
Ppu21I YACGTR 1 cut(s) 336
PpuMI RGGWCCY 1 cut(s) 1140
Psp5II RGGWCCY 1 cut(s) 1140
Psp6I CCWGG 2 cut(s) 619, 1241
PspGI CCWGG 2 cut(s) 619, 1241
PspN4I GGNNCC 5 cut(s) 277, 409, 773, 793, 1092
PspPI GGNCC 6 cut(s) 351, 356, 517, 720, 1091, 1140
PspPPI RGGWCCY 1 cut(s) 1140
PstI CTGCAG 1 cut(s) 1006
PsyI GACNNNGTC 1 cut(s) 383
RsaI GTAC 3 cut(s) 556, 635, 820
RsaNI GTAC 3 cut(s) 555, 634, 819
RseI CAYNNNNRTG 2 cut(s) 90, 931
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 8 cut(s) 207, 210, 213, 216, 321, 546, 700, 1002
Sau3AI GATC 2 cut(s) 607, 766
Sau96I GGNCC 6 cut(s) 351, 356, 517, 720, 1091, 1140
SchI GAGTC 2 cut(s) 270, 949
ScrFI CCNGG 4 cut(s) 354, 621, 1125, 1243
SduI GDGCHC 1 cut(s) 627
SfaNI GCATC 2 cut(s) 935, 1043
SfcI CTRYAG 2 cut(s) 184, 1002
SfoI GGCGCC 1 cut(s) 409
SinI GGWCC 2 cut(s) 517, 1140
SmiMI CAYNNNNRTG 2 cut(s) 90, 931
SmlI CTYRAG 2 cut(s) 130, 1172
SmoI CTYRAG 2 cut(s) 130, 1172
SphI GCATGC 1 cut(s) 84
Sse9I AATT 4 cut(s) 833, 901, 945, 1075
SsiI CCGC 2 cut(s) 321, 893
SspDI GGCGCC 1 cut(s) 407
SspI AATATT 2 cut(s) 513, 880
SspMI CTAG 4 cut(s) 203, 963, 992, 1190
StyD4I CCNGG 4 cut(s) 352, 619, 1123, 1241
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 8 cut(s) 226, 385, 484, 559, 762, 796, 818, 850
TaiI ACGT 3 cut(s) 338, 661, 1215
TaqI TCGA 5 cut(s) 23, 477, 534, 666, 1236
TasI AATT 4 cut(s) 833, 901, 945, 1075
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 4 cut(s) 966, 1009, 1130, 1238
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TscAI CASTG 3 cut(s) 982, 1089, 1203
TseFI GTSAC 1 cut(s) 1249
TseI GCWGC 7 cut(s) 206, 209, 212, 215, 545, 699, 1001
Tsp45I GTSAC 1 cut(s) 1249
TspDTI ATGAA 4 cut(s) 277, 353, 486, 872
TspGWI ACGGA 1 cut(s) 836
TspRI CASTG 3 cut(s) 982, 1089, 1203
Tth111I GACNNNGTC 1 cut(s) 383
VpaK11BI GGWCC 2 cut(s) 517, 1140
XapI RAATTY 2 cut(s) 833, 1075
XceI RCATGY 1 cut(s) 84
XmiI GTMKAC 2 cut(s) 813, 1015
XspI CTAG 4 cut(s) 203, 963, 992, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.