Rw4G030100

Leucoanthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016318
Physical Location & Seq
Forward (+)
56784504 .. 56787537
3034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030100.1

Sequence Viewer

Length: 1053 bp
ATGACGGTGTCACCTTCTATTGCCTCTGCTGCCAAGAGTGGCCGAGTCCTCATAATCGGCGCCACCGGCTTCATCGGCAAGTTCGTCGCCGAAGCCAGCCTAGACTCAGGCCTGCCTACTTACGTCCTCGTCAGACCCGGCCCGTCTCGCCCTCCCAAGTCCGACATCATCAAGTCGTTCAAAGACAGAGGCGCCATCATCCTACATGGAGTGATGAGTGATAGGCCATTGATGGAGAAGCTGCTGAGAGAGCATGAGATCGACATAATCATATCAGCCGTGGGTGGTGCAACAATTTTGGACCAGATTACCCTGATCGAAGCCATCGCTGCAGTCGGAACGGTCAAGAGGTTTCTGCCGTCGGAGTTCGGGCACGATGTGGACCGGGCCGACCCGGTGGAACCGGGTCTGACGATGTACTTGGAGAAGCGCAAGGTGAGGCGTGCGATTGAGAAGACTGAGGTCCCTTACACCTACATTTGTTGCAATTCTATTGCTTCTTGGCCTTACTACGACAACAAGCATCCTTCAGAGGTTGTTCCGCCGTTGGATCAGTTCCAGATCTACGGTGACGGTACGGTCAAAGCCTACTTCGTAGACGGTCCTGATATTGGAAAGTTCACCATGAAGACGGTGGACGACATCCGAGCCATAAACAAAAATGTTCACTTTCGACCCTCAAGCAATCTGTATGATATCAACGGTCTTGCTTCTTTGTGGGAGAAGAAAATTGGTCGGACCCTCCCGAGAGTTACTGTAACTGAACACGACCTATTGTCAATGGCTGCAGAGAACCGTATACCAGAAAGTATTGTAGCCTCATTCACTCATGATATCTTTATCAAAGGCTGTCAAACCAATTTCCCCATTGAAGGTCCTAATGATATTGATGTGGGCACTCTATATCCTGAGGAATCTTTTAGGACCTTGGATGAGTGCTTCAATGACTTCCTTGTCAAAGTTGGGGGGACGCTAGAGACTGATAAAATTGCCACCAAGAACACGGCGGCGGTTGGAGTTGAACCTATGGCAATCACTGCAACTTGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
NmrA

Protein Analysis

350

Amino Acids

38.36

Weight (kDa)

5.59

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 14 - 312 1.4e-53 NmrA-like family
Epimerase PF01370 16 - 107 3.5e-06 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016318)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g28110
malus_domestica MD13G1046900.v1.1 MD16G1048500.v1.1
prunus_persica Prupe.1G305100_v2.0.a1
pyrus_communis pycom13g04100 pycom16g04240
rosa_chinensis RchiOBHm_Chr4g0435881
rosa_laevigata RLG00000006553
rosa_multiflora Rmu_sc0003165.1_g000010
rosa_roxburghii Rroxscaffold_5G00377050
rosa_rugosa Rorug04G0290200
rosa_samantha Rh4BG353500 Rh4CG367900 Rh4DG347300
rosa_wichuraiana Rw4G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 578, 953
AccB1I GGYRCC 2 cut(s) 59, 191
AccI GTMKAC 2 cut(s) 597, 799
AciI CCGC 3 cut(s) 542, 1007, 1010
AclWI GGATC 1 cut(s) 558
AcoI YGGCCR 1 cut(s) 40
AcuI CTGAAG 1 cut(s) 513
AcyI GRCGYC 2 cut(s) 60, 192
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 2 cut(s) 419, 577
AfiI CCNNNNNNNGG 2 cut(s) 611, 872
AgsI TTSAA 4 cut(s) 181, 872, 943, 1022
AhdI GACNNNNNGTC 1 cut(s) 775
AluBI AGCT 1 cut(s) 241
AluI AGCT 1 cut(s) 241
Alw26I GTCTC 2 cut(s) 150, 971
AlwI GGATC 1 cut(s) 558
Ama87I CYCGRG 1 cut(s) 745
AoxI GGCC 6 cut(s) 40, 109, 139, 224, 387, 503
ApeKI GCWGC 4 cut(s) 29, 241, 329, 785
AspLEI GCGC 3 cut(s) 62, 194, 432
AspS9I GGNCC 9 cut(s) 140, 301, 382, 387, 463, 602, 738, 875, 924
AsuC2I CCSGG 4 cut(s) 138, 386, 395, 405
AsuHPI GGTGA 4 cut(s) 3, 448, 581, 613
AvaI CYCGRG 1 cut(s) 745
AvaII GGWCC 7 cut(s) 301, 382, 463, 602, 738, 875, 924
AxyI CCTNAGG 1 cut(s) 909
BaeGI GKGCMC 2 cut(s) 375, 899
BanI GGYRCC 2 cut(s) 59, 191
BbsI GAAGAC 2 cut(s) 461, 635
BbvI GCAGC 4 cut(s) 16, 228, 316, 772
BccI CCATC 3 cut(s) 203, 226, 332
BceAI ACGGC 4 cut(s) 263, 343, 529, 1020
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BcnI CCSGG 4 cut(s) 138, 386, 395, 405
BcoDI GTCTC 2 cut(s) 150, 971
BfaI CTAG 2 cut(s) 101, 974
BfmI CTRYAG 2 cut(s) 330, 786
BfoI RGCGCY 2 cut(s) 63, 195
BglII AGATCT 1 cut(s) 561
BisI GCNGC 5 cut(s) 30, 242, 330, 786, 1008
BlsI GCNGC 5 cut(s) 31, 243, 331, 787, 1009
Bme1390I CCNGG 4 cut(s) 138, 386, 395, 405
Bme18I GGWCC 7 cut(s) 301, 382, 463, 602, 738, 875, 924
BmeRI GACNNNNNGTC 1 cut(s) 775
BmeT110I CYCGRG 1 cut(s) 745
BmgT120I GGNCC 9 cut(s) 140, 301, 382, 387, 463, 602, 738, 875, 924
BmiI GGNNCC 5 cut(s) 61, 193, 402, 465, 740
BmrFI CCNGG 4 cut(s) 138, 386, 395, 405
BmsI GCATC 1 cut(s) 532
BoxI GACNNNNGTC 1 cut(s) 461
BpiI GAAGAC 2 cut(s) 461, 635
BpuEI CTTGAG 1 cut(s) 664
BpuMI CCSGG 4 cut(s) 138, 386, 395, 405
BsaHI GRCGYC 2 cut(s) 60, 192
BsaJI CCNNGG 2 cut(s) 279, 927
Bsc4I CCNNNNNNNGG 2 cut(s) 611, 872
Bse118I RCCGGY 1 cut(s) 65
Bse21I CCTNAGG 1 cut(s) 909
BseDI CCNNGG 2 cut(s) 279, 927
BseGI GGATG 4 cut(s) 198, 523, 642, 937
BseLI CCNNNNNNNGG 2 cut(s) 611, 872
BseMII CTCAG 4 cut(s) 120, 236, 450, 900
BseSI GKGCMC 2 cut(s) 375, 899
BseXI GCAGC 4 cut(s) 16, 228, 316, 772
BshFI GGCC 6 cut(s) 42, 111, 141, 226, 389, 505
BshNI GGYRCC 2 cut(s) 59, 191
BsiHKCI CYCGRG 1 cut(s) 745
BsiSI CCGG 5 cut(s) 66, 138, 385, 395, 404
BslFI GGGAC 2 cut(s) 449, 982
BslI CCNNNNNNNGG 2 cut(s) 611, 872
BsmAI GTCTC 2 cut(s) 150, 971
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 2 cut(s) 449, 982
BsnI GGCC 6 cut(s) 42, 111, 141, 226, 389, 505
BsoBI CYCGRG 1 cut(s) 745
Bsp1286I GDGCHC 2 cut(s) 375, 899
Bsp143I GATC 4 cut(s) 258, 315, 550, 561
BspACI CCGC 3 cut(s) 542, 1007, 1010
BspANI GGCC 6 cut(s) 42, 111, 141, 226, 389, 505
BspCNI CTCAG 4 cut(s) 119, 237, 451, 901
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 5 cut(s) 61, 193, 402, 465, 740
BspMAI CTGCAG 2 cut(s) 334, 790
BspPI GGATC 1 cut(s) 558
BspT107I GGYRCC 2 cut(s) 59, 191
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 279, 927
BssMI GATC 4 cut(s) 258, 315, 550, 561
BssNAI GTATAC 1 cut(s) 800
BssNI GRCGYC 2 cut(s) 60, 192
BssT1I CCWWGG 1 cut(s) 927
Bst1107I GTATAC 1 cut(s) 800
BstACI GRCGYC 2 cut(s) 60, 192
BstAPI GCANNNNNTGC 2 cut(s) 1037, 1046
BstC8I GCNNGC 3 cut(s) 97, 113, 444
BstDEI CTNAG 4 cut(s) 106, 245, 459, 909
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 4 cut(s) 198, 523, 642, 937
BstH2I RGCGCY 2 cut(s) 63, 195
BstHHI GCGC 3 cut(s) 62, 194, 432
BstKTI GATC 4 cut(s) 261, 318, 553, 564
BstMAI GTCTC 2 cut(s) 150, 971
BstMBI GATC 4 cut(s) 258, 315, 550, 561
BstMWI GCNNNNNNNGC 8 cut(s) 29, 66, 75, 147, 250, 329, 1037, 1046
BstPAI GACNNNNGTC 1 cut(s) 461
BstSCI CCNGG 4 cut(s) 136, 384, 393, 403
BstSFI CTRYAG 2 cut(s) 330, 786
BstSLI GKGCMC 2 cut(s) 375, 899
BstV1I GCAGC 4 cut(s) 16, 228, 316, 772
BstV2I GAAGAC 2 cut(s) 461, 635
BstX2I RGATCY 1 cut(s) 561
BstYI RGATCY 1 cut(s) 561
BstZ17I GTATAC 1 cut(s) 800
Bsu36I CCTNAGG 1 cut(s) 909
BsuRI GGCC 6 cut(s) 42, 111, 141, 226, 389, 505
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 310
BtsCI GGATG 4 cut(s) 198, 523, 642, 937
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 1 cut(s) 1035
Cac8I GCNNGC 3 cut(s) 97, 113, 444
CciI TCATGA 1 cut(s) 829
CfoI GCGC 3 cut(s) 62, 194, 432
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 9 cut(s) 140, 301, 382, 387, 463, 602, 738, 875, 924
CseI GACGC 1 cut(s) 979
Csp6I GTAC 2 cut(s) 418, 576
CviAII CATG 4 cut(s) 206, 254, 625, 830
CviQI GTAC 2 cut(s) 418, 576
DdeI CTNAG 4 cut(s) 106, 245, 459, 909
DinI GGCGCC 2 cut(s) 61, 193
DpnI GATC 4 cut(s) 260, 317, 552, 563
DpnII GATC 4 cut(s) 258, 315, 550, 561
DraIII CACNNNGTG 1 cut(s) 379
DrdI GACNNNNNNGTC 2 cut(s) 578, 953
DriI GACNNNNNGTC 1 cut(s) 775
DseDI GACNNNNNNGTC 2 cut(s) 578, 953
EaeI YGGCCR 1 cut(s) 40
Eam1105I GACNNNNNGTC 1 cut(s) 775
EciI GGCGGA 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 927
Eco147I AGGCCT 1 cut(s) 111
Eco32I GATATC 2 cut(s) 697, 835
Eco47I GGWCC 7 cut(s) 301, 382, 463, 602, 738, 875, 924
Eco57I CTGAAG 1 cut(s) 513
Eco81I CCTNAGG 1 cut(s) 909
Eco88I CYCGRG 1 cut(s) 745
EcoO109I RGGNCCY 3 cut(s) 463, 875, 924
EcoRV GATATC 2 cut(s) 697, 835
EcoT14I CCWWGG 1 cut(s) 927
EgeI GGCGCC 2 cut(s) 61, 193
EheI GGCGCC 2 cut(s) 61, 193
ErhI CCWWGG 1 cut(s) 927
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 4 cut(s) 209, 257, 628, 833
FaqI GGGAC 2 cut(s) 449, 982
FatI CATG 4 cut(s) 205, 253, 624, 829
FblI GTMKAC 2 cut(s) 597, 799
Fnu4HI GCNGC 5 cut(s) 30, 242, 330, 786, 1008
FokI GGATG 4 cut(s) 185, 510, 629, 944
Fsp4HI GCNGC 5 cut(s) 30, 242, 330, 786, 1008
FspBI CTAG 2 cut(s) 101, 974
GlaI GCGC 3 cut(s) 61, 193, 431
GluI GCNGC 5 cut(s) 30, 242, 330, 786, 1008
HaeII RGCGCY 2 cut(s) 63, 195
HaeIII GGCC 6 cut(s) 42, 111, 141, 226, 389, 505
HapII CCGG 5 cut(s) 66, 138, 385, 395, 404
HgaI GACGC 1 cut(s) 979
HhaI GCGC 3 cut(s) 62, 194, 432
Hin1I GRCGYC 2 cut(s) 60, 192
Hin1II CATG 4 cut(s) 209, 257, 628, 833
Hin6I GCGC 3 cut(s) 60, 192, 430
HinP1I GCGC 3 cut(s) 60, 192, 430
HinfI GANTC 3 cut(s) 45, 104, 914
HpaII CCGG 5 cut(s) 66, 138, 385, 395, 404
HphI GGTGA 4 cut(s) 3, 448, 581, 613
Hpy166II GTNNAC 6 cut(s) 382, 598, 621, 637, 667, 800
Hpy188I TCNGA 8 cut(s) 134, 163, 338, 364, 411, 532, 647, 738
Hpy188III TCNNGA 6 cut(s) 346, 559, 605, 745, 830, 908
Hpy8I GTNNAC 6 cut(s) 382, 598, 621, 637, 667, 800
Hpy99I CGWCG 2 cut(s) 89, 364
HpyAV CCTTC 3 cut(s) 24, 537, 866
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 290, 332, 486, 788, 1040
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 66, 75, 147, 250, 329, 1037, 1046
HpyF3I CTNAG 4 cut(s) 106, 245, 459, 909
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 2 cut(s) 60, 192
Hsp92II CATG 4 cut(s) 209, 257, 628, 833
HspAI GCGC 3 cut(s) 60, 192, 430
KasI GGCGCC 2 cut(s) 59, 191
Kzo9I GATC 4 cut(s) 258, 315, 550, 561
Lsp1109I GCAGC 4 cut(s) 16, 228, 316, 772
LweI GCATC 1 cut(s) 532
MaeI CTAG 2 cut(s) 101, 974
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 4 cut(s) 9, 569, 751, 757
MalI GATC 4 cut(s) 260, 317, 552, 563
MboI GATC 4 cut(s) 258, 315, 550, 561
MboII GAAGA 3 cut(s) 466, 640, 736
MflI RGATCY 1 cut(s) 561
MhlI GDGCHC 2 cut(s) 375, 899
MluCI AATT 5 cut(s) 294, 487, 729, 859, 987
Mly113I GGCGCC 2 cut(s) 60, 192
MlyI GAGTC 2 cut(s) 54, 98
MmeI TCCRAC 6 cut(s) 186, 316, 342, 528, 716, 994
MspI CCGG 5 cut(s) 66, 138, 385, 395, 404
MspR9I CCNGG 4 cut(s) 138, 386, 395, 405
MwoI GCNNNNNNNGC 8 cut(s) 29, 66, 75, 147, 250, 329, 1037, 1046
NarI GGCGCC 2 cut(s) 60, 192
NciI CCSGG 4 cut(s) 138, 386, 395, 405
NdeII GATC 4 cut(s) 258, 315, 550, 561
NlaIII CATG 4 cut(s) 209, 257, 628, 833
NlaIV GGNNCC 5 cut(s) 61, 193, 402, 465, 740
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 2 cut(s) 9, 569
PagI TCATGA 1 cut(s) 829
PceI AGGCCT 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 1 cut(s) 914
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 5 cut(s) 31, 243, 331, 787, 1009
PleI GAGTC 2 cut(s) 53, 98
PluTI GGCGCC 2 cut(s) 63, 195
PpsI GAGTC 2 cut(s) 53, 98
PpuMI RGGWCCY 3 cut(s) 463, 875, 924
PshAI GACNNNNGTC 1 cut(s) 461
Psp5II RGGWCCY 3 cut(s) 463, 875, 924
PspN4I GGNNCC 5 cut(s) 61, 193, 402, 465, 740
PspPI GGNCC 9 cut(s) 140, 301, 382, 387, 463, 602, 738, 875, 924
PspPPI RGGWCCY 3 cut(s) 463, 875, 924
PstI CTGCAG 2 cut(s) 334, 790
PsuI RGATCY 1 cut(s) 561
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 2 cut(s) 419, 577
RsaNI GTAC 2 cut(s) 418, 576
SatI GCNGC 5 cut(s) 30, 242, 330, 786, 1008
Sau3AI GATC 4 cut(s) 258, 315, 550, 561
Sau96I GGNCC 9 cut(s) 140, 301, 382, 387, 463, 602, 738, 875, 924
SchI GAGTC 2 cut(s) 54, 98
ScrFI CCNGG 4 cut(s) 138, 386, 395, 405
SduI GDGCHC 2 cut(s) 375, 899
SfaNI GCATC 1 cut(s) 532
SfcI CTRYAG 2 cut(s) 330, 786
SfoI GGCGCC 2 cut(s) 61, 193
SinI GGWCC 7 cut(s) 301, 382, 463, 602, 738, 875, 924
SmlI CTYRAG 1 cut(s) 679
SmoI CTYRAG 1 cut(s) 679
Sse9I AATT 5 cut(s) 294, 487, 729, 859, 987
SseBI AGGCCT 1 cut(s) 111
SsiI CCGC 3 cut(s) 542, 1007, 1010
SspDI GGCGCC 2 cut(s) 59, 191
SspMI CTAG 2 cut(s) 101, 974
StuI AGGCCT 1 cut(s) 111
StyD4I CCNGG 4 cut(s) 136, 384, 393, 403
StyI CCWWGG 1 cut(s) 927
TaiI ACGT 1 cut(s) 126
TaqI TCGA 3 cut(s) 261, 318, 673
TasI AATT 5 cut(s) 294, 487, 729, 859, 987
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 1010
TfiI GAWTC 1 cut(s) 914
TscAI CASTG 1 cut(s) 1042
TseFI GTSAC 2 cut(s) 9, 569
TseI GCWGC 4 cut(s) 29, 241, 329, 785
Tsp45I GTSAC 2 cut(s) 9, 569
TspDTI ATGAA 2 cut(s) 61, 641
TspRI CASTG 1 cut(s) 1042
Tth111I GACNNNGTC 1 cut(s) 7
VpaK11BI GGWCC 7 cut(s) 301, 382, 463, 602, 738, 875, 924
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XmiI GTMKAC 2 cut(s) 597, 799
XspI CTAG 2 cut(s) 101, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.