Rroxscaffold_5G00377050

Leucoanthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016318
Physical Location & Seq
Forward (+)
57610523 .. 57613220
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00377050.1

Sequence Viewer

Length: 1059 bp
ATGACGGTGTCACCTTCTATTGCCTCTGCTGCCAAGAGTGGCCGAGTCCTCATAATCGGCGCCACCGGCTTCATCGGCAAGTTCGTCGCCGAAGCCAGTCTAGACTCAGGCCTGCCTACTTACGTCCTCGTCCGATCCGGCCCGTCTCGCCCTCCCAAGTCCGACACCATCAAGTCGTTCAAAGACAGAGGCGCCATCATCCTACATGGAGTGATGAGTGATAGGCCATTGATGGAGAAGCTGCTGAGAGAGCATGAGATCGACATAGTCATATCAGCCGTGGGTGGTGCAACAATTTTGGACCAGATTACCCTGATCGAAGCCATCGCTGCAGTCGGAACGGTCAAGGTAAATCTGCTTTTTGCGAATCAATGTGTTTGTTTCTCTTTGACCAGAAAACAAAAACTGACCACCGTTAGTTTCTTGGTGCAGAGGTTTCTGCCGTCGGAGTTCGGGCACGATGTGGACCGGGCCGACCCGGTGGAACCGGGTCTGACGATGTACTTGGAGAAGCGCCAGGTGAGGCGTGCGATTGAGAAGACTGGAGTCCCTTACACCTACATTTGTTGCAATTCTATTGCTTCTTGGCCTTACTACGACAACAAGCATCCTGCAGAGGTTGTTCCGCCGTTGGATCAGTTCCAGATCTACGGTGACGGTACAGTCAAAGCCTACTTCGTAGATGGTCCTGATATTGGAAAGTTCACCATGAAAACGGTGGACGACGTCCGAGCCATAAACAAAAATGTTCACTTTCGACCCGCAAGCAATTTGTATGATATCAACGGTCTTGCTTCTTTGTGGGAGAAGAAAATTGGTCGGACCCTCCCGAGAGTTACTGTAACTGAACATGACCTATTGGCAATGGCTGCAGAGAACCGTATACCAGAAAGTATTGTAGCCTCATTCACTCATGATATCTTTATCAAAGGCTGTCAAACCAATTTCCCCATTGAAGGTCCTAATGATATTGATGTGGGCACTCTATATCCTGAAGAATCTTTTAGGACCTTGGATGAGTGCTTCAATGACTTTCTTGTCAAAGTTGGGGGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
NmrA

Protein Analysis

352

Amino Acids

38.76

Weight (kDa)

6.19

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 14 - 116 2.4e-18 NmrA-like family
Epimerase PF01370 16 - 110 3.9e-07 NAD dependent epimerase/dehydratase family
NmrA PF05368 143 - 340 3.9e-30 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016318)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g28110
malus_domestica MD13G1046900.v1.1 MD16G1048500.v1.1
prunus_persica Prupe.1G305100_v2.0.a1
pyrus_communis pycom13g04100 pycom16g04240
rosa_chinensis RchiOBHm_Chr4g0435881
rosa_laevigata RLG00000006553
rosa_multiflora Rmu_sc0003165.1_g000010
rosa_roxburghii Rroxscaffold_5G00377050
rosa_rugosa Rorug04G0290200
rosa_samantha Rh4BG353500 Rh4CG367900 Rh4DG347300
rosa_wichuraiana Rw4G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 662, 1037
AatII GACGTC 1 cut(s) 729
AccB1I GGYRCC 2 cut(s) 59, 191
AccI GTMKAC 1 cut(s) 883
AciI CCGC 2 cut(s) 626, 762
AclWI GGATC 2 cut(s) 129, 642
AcoI YGGCCR 1 cut(s) 40
AcuI CTGAAG 1 cut(s) 1014
AcyI GRCGYC 3 cut(s) 60, 192, 726
AdeI CACNNNGTG 1 cut(s) 463
AfaI GTAC 2 cut(s) 503, 661
AfiI CCNNNNNNNGG 2 cut(s) 695, 956
AgsI TTSAA 3 cut(s) 181, 956, 1027
AjnI CCWGG 1 cut(s) 516
AluBI AGCT 2 cut(s) 241, 1056
AluI AGCT 2 cut(s) 241, 1056
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 2 cut(s) 129, 642
Ama87I CYCGRG 1 cut(s) 829
AoxI GGCC 6 cut(s) 40, 109, 139, 224, 471, 587
ApeKI GCWGC 4 cut(s) 29, 241, 329, 869
AspLEI GCGC 3 cut(s) 62, 194, 516
AspS9I GGNCC 8 cut(s) 140, 301, 466, 471, 686, 822, 959, 1008
AsuC2I CCSGG 3 cut(s) 470, 479, 489
AsuHPI GGTGA 4 cut(s) 3, 532, 665, 697
AvaI CYCGRG 1 cut(s) 829
AvaII GGWCC 6 cut(s) 301, 466, 686, 822, 959, 1008
BaeGI GKGCMC 2 cut(s) 459, 983
BanI GGYRCC 2 cut(s) 59, 191
BbsI GAAGAC 1 cut(s) 545
BbvI GCAGC 4 cut(s) 16, 228, 316, 856
BccI CCATC 5 cut(s) 176, 203, 226, 332, 677
BceAI ACGGC 3 cut(s) 263, 427, 613
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 1 cut(s) 518
BcnI CCSGG 3 cut(s) 470, 479, 489
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 2 cut(s) 101, 1057
BfmI CTRYAG 3 cut(s) 330, 612, 870
BfoI RGCGCY 3 cut(s) 63, 195, 517
BglII AGATCT 1 cut(s) 645
BisI GCNGC 4 cut(s) 30, 242, 330, 870
BlsI GCNGC 4 cut(s) 31, 243, 331, 871
Bme1390I CCNGG 4 cut(s) 470, 479, 489, 518
Bme18I GGWCC 6 cut(s) 301, 466, 686, 822, 959, 1008
BmeT110I CYCGRG 1 cut(s) 829
BmgT120I GGNCC 8 cut(s) 140, 301, 466, 471, 686, 822, 959, 1008
BmiI GGNNCC 4 cut(s) 61, 193, 486, 824
BmrFI CCNGG 4 cut(s) 470, 479, 489, 518
BmsI GCATC 1 cut(s) 616
BoxI GACNNNNGTC 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 545
BpmI CTGGAG 1 cut(s) 564
BpuMI CCSGG 3 cut(s) 470, 479, 489
BsaHI GRCGYC 3 cut(s) 60, 192, 726
BsaJI CCNNGG 2 cut(s) 279, 1011
Bsc4I CCNNNNNNNGG 2 cut(s) 695, 956
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 96, 547
Bse3DI GCAATG 1 cut(s) 870
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 2 cut(s) 279, 1011
BseGI GGATG 3 cut(s) 198, 607, 1021
BseLI CCNNNNNNNGG 2 cut(s) 695, 956
BseMI GCAATG 1 cut(s) 870
BseMII CTCAG 2 cut(s) 120, 236
BseNI ACTGG 2 cut(s) 96, 547
BseSI GKGCMC 2 cut(s) 459, 983
BseXI GCAGC 4 cut(s) 16, 228, 316, 856
BsgI GTGCAG 1 cut(s) 449
BshFI GGCC 6 cut(s) 42, 111, 141, 226, 473, 589
BshNI GGYRCC 2 cut(s) 59, 191
BsiHKCI CYCGRG 1 cut(s) 829
BsiSI CCGG 5 cut(s) 66, 138, 469, 479, 488
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 2 cut(s) 695, 956
BsmAI GTCTC 1 cut(s) 150
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 533
BsnI GGCC 6 cut(s) 42, 111, 141, 226, 473, 589
BsoBI CYCGRG 1 cut(s) 829
Bsp1286I GDGCHC 2 cut(s) 459, 983
Bsp143I GATC 5 cut(s) 134, 258, 315, 634, 645
BspACI CCGC 2 cut(s) 626, 762
BspANI GGCC 6 cut(s) 42, 111, 141, 226, 473, 589
BspCNI CTCAG 2 cut(s) 119, 237
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 4 cut(s) 61, 193, 486, 824
BspMAI CTGCAG 3 cut(s) 334, 616, 874
BspPI GGATC 2 cut(s) 129, 642
BspT107I GGYRCC 2 cut(s) 59, 191
BsrDI GCAATG 1 cut(s) 870
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 2 cut(s) 96, 547
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 279, 1011
BssMI GATC 5 cut(s) 134, 258, 315, 634, 645
BssNAI GTATAC 1 cut(s) 884
BssNI GRCGYC 3 cut(s) 60, 192, 726
BssT1I CCWWGG 1 cut(s) 1011
Bst1107I GTATAC 1 cut(s) 884
Bst2UI CCWGG 1 cut(s) 518
BstACI GRCGYC 3 cut(s) 60, 192, 726
BstAPI GCANNNNNTGC 1 cut(s) 869
BstC8I GCNNGC 3 cut(s) 113, 528, 766
BstDEI CTNAG 2 cut(s) 106, 245
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 198, 607, 1021
BstH2I RGCGCY 3 cut(s) 63, 195, 517
BstHHI GCGC 3 cut(s) 62, 194, 516
BstKTI GATC 5 cut(s) 137, 261, 318, 637, 648
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 5 cut(s) 134, 258, 315, 634, 645
BstMWI GCNNNNNNNGC 7 cut(s) 29, 66, 75, 147, 250, 329, 869
BstNI CCWGG 1 cut(s) 518
BstPAI GACNNNNGTC 1 cut(s) 545
BstSCI CCNGG 4 cut(s) 468, 477, 487, 516
BstSFI CTRYAG 3 cut(s) 330, 612, 870
BstSLI GKGCMC 2 cut(s) 459, 983
BstV1I GCAGC 4 cut(s) 16, 228, 316, 856
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 645
BstYI RGATCY 1 cut(s) 645
BstZ17I GTATAC 1 cut(s) 884
BsuRI GGCC 6 cut(s) 42, 111, 141, 226, 473, 589
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 310
BtsCI GGATG 3 cut(s) 198, 607, 1021
Cac8I GCNNGC 3 cut(s) 113, 528, 766
CciI TCATGA 1 cut(s) 913
CfoI GCGC 3 cut(s) 62, 194, 516
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 8 cut(s) 140, 301, 466, 471, 686, 822, 959, 1008
Csp6I GTAC 2 cut(s) 502, 660
CviAII CATG 5 cut(s) 206, 254, 709, 851, 914
CviQI GTAC 2 cut(s) 502, 660
DdeI CTNAG 2 cut(s) 106, 245
DinI GGCGCC 2 cut(s) 61, 193
DpnI GATC 5 cut(s) 136, 260, 317, 636, 647
DpnII GATC 5 cut(s) 134, 258, 315, 634, 645
DraIII CACNNNGTG 1 cut(s) 463
DrdI GACNNNNNNGTC 2 cut(s) 662, 1037
DseDI GACNNNNNNGTC 2 cut(s) 662, 1037
EaeI YGGCCR 1 cut(s) 40
EciI GGCGGA 1 cut(s) 615
Eco130I CCWWGG 1 cut(s) 1011
Eco147I AGGCCT 1 cut(s) 111
Eco32I GATATC 2 cut(s) 781, 919
Eco47I GGWCC 6 cut(s) 301, 466, 686, 822, 959, 1008
Eco57I CTGAAG 1 cut(s) 1014
Eco88I CYCGRG 1 cut(s) 829
EcoO109I RGGNCCY 2 cut(s) 959, 1008
EcoRII CCWGG 1 cut(s) 516
EcoRV GATATC 2 cut(s) 781, 919
EcoT14I CCWWGG 1 cut(s) 1011
EgeI GGCGCC 2 cut(s) 61, 193
EheI GGCGCC 2 cut(s) 61, 193
ErhI CCWWGG 1 cut(s) 1011
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 5 cut(s) 209, 257, 712, 854, 917
FaqI GGGAC 1 cut(s) 533
FatI CATG 5 cut(s) 205, 253, 708, 850, 913
FauI CCCGC 1 cut(s) 769
FblI GTMKAC 1 cut(s) 883
Fnu4HI GCNGC 4 cut(s) 30, 242, 330, 870
FokI GGATG 3 cut(s) 185, 594, 1028
Fsp4HI GCNGC 4 cut(s) 30, 242, 330, 870
FspBI CTAG 2 cut(s) 101, 1057
GlaI GCGC 3 cut(s) 61, 193, 515
GluI GCNGC 4 cut(s) 30, 242, 330, 870
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 3 cut(s) 63, 195, 517
HaeIII GGCC 6 cut(s) 42, 111, 141, 226, 473, 589
HapII CCGG 5 cut(s) 66, 138, 469, 479, 488
HhaI GCGC 3 cut(s) 62, 194, 516
Hin1I GRCGYC 3 cut(s) 60, 192, 726
Hin1II CATG 5 cut(s) 209, 257, 712, 854, 917
Hin6I GCGC 3 cut(s) 60, 192, 514
HinP1I GCGC 3 cut(s) 60, 192, 514
HinfI GANTC 5 cut(s) 45, 104, 367, 546, 998
HpaII CCGG 5 cut(s) 66, 138, 469, 479, 488
HphI GGTGA 4 cut(s) 3, 532, 665, 697
Hpy166II GTNNAC 5 cut(s) 466, 705, 721, 751, 884
Hpy188I TCNGA 7 cut(s) 134, 163, 338, 448, 495, 731, 822
Hpy188III TCNNGA 6 cut(s) 101, 643, 689, 829, 914, 992
Hpy8I GTNNAC 5 cut(s) 466, 705, 721, 751, 884
Hpy99I CGWCG 3 cut(s) 89, 448, 728
HpyAV CCTTC 2 cut(s) 24, 950
HpyCH4IV ACGT 2 cut(s) 123, 726
HpyCH4V TGCA 6 cut(s) 290, 332, 430, 570, 614, 872
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 66, 75, 147, 250, 329, 869
HpyF3I CTNAG 2 cut(s) 106, 245
HpySE526I ACGT 2 cut(s) 123, 726
Hsp92I GRCGYC 3 cut(s) 60, 192, 726
Hsp92II CATG 5 cut(s) 209, 257, 712, 854, 917
HspAI GCGC 3 cut(s) 60, 192, 514
KasI GGCGCC 2 cut(s) 59, 191
Kzo9I GATC 5 cut(s) 134, 258, 315, 634, 645
Lsp1109I GCAGC 4 cut(s) 16, 228, 316, 856
LweI GCATC 1 cut(s) 616
MaeI CTAG 2 cut(s) 101, 1057
MaeII ACGT 2 cut(s) 123, 726
MaeIII GTNAC 4 cut(s) 9, 653, 835, 841
MalI GATC 5 cut(s) 136, 260, 317, 636, 647
MboI GATC 5 cut(s) 134, 258, 315, 634, 645
MboII GAAGA 3 cut(s) 550, 820, 1007
MflI RGATCY 1 cut(s) 645
MhlI GDGCHC 2 cut(s) 459, 983
MluCI AATT 5 cut(s) 294, 571, 769, 813, 943
Mly113I GGCGCC 2 cut(s) 60, 192
MlyI GAGTC 3 cut(s) 54, 98, 555
MmeI TCCRAC 5 cut(s) 186, 316, 426, 612, 800
MspI CCGG 5 cut(s) 66, 138, 469, 479, 488
MspR9I CCNGG 4 cut(s) 470, 479, 489, 518
MvaI CCWGG 1 cut(s) 518
MwoI GCNNNNNNNGC 7 cut(s) 29, 66, 75, 147, 250, 329, 869
NarI GGCGCC 2 cut(s) 60, 192
NciI CCSGG 3 cut(s) 470, 479, 489
NdeII GATC 5 cut(s) 134, 258, 315, 634, 645
NlaIII CATG 5 cut(s) 209, 257, 712, 854, 917
NlaIV GGNNCC 4 cut(s) 61, 193, 486, 824
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 2 cut(s) 9, 653
PagI TCATGA 1 cut(s) 913
PceI AGGCCT 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 2 cut(s) 367, 998
PflFI GACNNNGTC 3 cut(s) 7, 266, 725
PkrI GCNGC 4 cut(s) 31, 243, 331, 871
PleI GAGTC 3 cut(s) 53, 98, 554
PluTI GGCGCC 2 cut(s) 63, 195
PpsI GAGTC 3 cut(s) 53, 98, 554
PpuMI RGGWCCY 2 cut(s) 959, 1008
PshAI GACNNNNGTC 1 cut(s) 545
Psp5II RGGWCCY 2 cut(s) 959, 1008
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 4 cut(s) 61, 193, 486, 824
PspPI GGNCC 8 cut(s) 140, 301, 466, 471, 686, 822, 959, 1008
PspPPI RGGWCCY 2 cut(s) 959, 1008
PstI CTGCAG 3 cut(s) 334, 616, 874
PsuI RGATCY 1 cut(s) 645
PsyI GACNNNGTC 3 cut(s) 7, 266, 725
RsaI GTAC 2 cut(s) 503, 661
RsaNI GTAC 2 cut(s) 502, 660
SatI GCNGC 4 cut(s) 30, 242, 330, 870
Sau3AI GATC 5 cut(s) 134, 258, 315, 634, 645
Sau96I GGNCC 8 cut(s) 140, 301, 466, 471, 686, 822, 959, 1008
SchI GAGTC 3 cut(s) 54, 98, 555
ScrFI CCNGG 4 cut(s) 470, 479, 489, 518
SduI GDGCHC 2 cut(s) 459, 983
SfaNI GCATC 1 cut(s) 616
SfcI CTRYAG 3 cut(s) 330, 612, 870
SfoI GGCGCC 2 cut(s) 61, 193
SinI GGWCC 6 cut(s) 301, 466, 686, 822, 959, 1008
Sse9I AATT 5 cut(s) 294, 571, 769, 813, 943
SseBI AGGCCT 1 cut(s) 111
SsiI CCGC 2 cut(s) 626, 762
SspDI GGCGCC 2 cut(s) 59, 191
SspMI CTAG 2 cut(s) 101, 1057
StuI AGGCCT 1 cut(s) 111
StyD4I CCNGG 4 cut(s) 468, 477, 487, 516
StyI CCWWGG 1 cut(s) 1011
TaiI ACGT 2 cut(s) 126, 729
TaqI TCGA 3 cut(s) 261, 318, 757
TasI AATT 5 cut(s) 294, 571, 769, 813, 943
TatI WGTACW 1 cut(s) 501
TfiI GAWTC 2 cut(s) 367, 998
TseFI GTSAC 2 cut(s) 9, 653
TseI GCWGC 4 cut(s) 29, 241, 329, 869
Tsp45I GTSAC 2 cut(s) 9, 653
TspDTI ATGAA 2 cut(s) 61, 725
Tth111I GACNNNGTC 3 cut(s) 7, 266, 725
VpaK11BI GGWCC 6 cut(s) 301, 466, 686, 822, 959, 1008
XbaI TCTAGA 1 cut(s) 100
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XmiI GTMKAC 1 cut(s) 883
XspI CTAG 2 cut(s) 101, 1057
ZraI GACGTC 1 cut(s) 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.