pycom13g04100

Leucoanthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0016318
Physical Location & Seq
Forward (+)
2746572 .. 2750360
3789 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g04100.1

Sequence Viewer

Length: 1269 bp
ATGGCAAAAATAGTAACAAAATCAAAGTATCCATCTATATATAGACAACATATATTCCTCCCTTACTTCACACACTTCACATGCCCCAATCGTGTCAAAAATAACTTGGGAAAAAAGGACTTTTCAAACTTGAGGGCAAAAAAAAACATACAACGAAGACGAAAAGTCAAGGCACTCTGTAGCAGGAAGGAAGAGCTAGCAGCAGCAGCCATGACTGTTTCACCTTCTCTTTCTGTTGCCATAAATGGCAGAGTCCTCATTGTCGGTGCCACCGGTTTCATCGGGAGGTTTGTCGCTGAAGCCAGCCTCGCCGCCGGCCAACCCACGTATGTTCTCGTCCGGCCTGGCCCTCTTCACCCTTCTAAGGCTGACACCGTCAAGTCCTTCAAACACAAAGGCGCTATAATCTTACACGGGCTGATTTCTGATAAAACCCTAATGGAGAAGATACTGAGAGAGCATGAAATCGAGACAGTAATATCAGCCGTCGGCGGTGCGACAATATTGGACCAGATTGCCCTAGTCGAGGCCATCGCAGCAGTTGGTACAGTTAAGAGGTTTTTGCCTTCGGAGTTTGGACATGATGTGGACAGGGCTGATCCGGTTGAACCAGGGCTCACCATGTATGTGGAGAAACGCAAGGTTAGACGTTGGGTCGAGAAATCTGGGGTGCCCTACACATACATTTGCTGCAACTCCATTGCCTCTTGGCCCTACTTTGACAACACACACCCTTCTGAGGTTCTCCCACCGTTGGATCGGTTCCAAATCTACGGTGATGGCACCGTCAAAGCCTATTTTGTAGACGGTACGGATATTGGAAAATTCACAATAAAGACCGCGAAGGACATCCGAACAATCAACAAAAATGTTCATTTCCGACCGCCGAGCAATTTATACGACATCAACGGGCTTGCATCTCTGTGGGAGAAGAAAATTGGCCGGACTCTCCCTAGAATCACCATCACTGAAAATCACTTACTAGCCCTTGCTGCAGAGAATCGTATACCAGAAAGTATAGTGGCATCGTTCACGCATGACATCTTTATCAAAGGATGTCAGGTGAATTTTGCAGTGGAGGGGCCTCGTGATGTGGAAGTGGGAACTCTCTACCCTGGAGATTCTTTCAGGACCTTGGATGAGTGCTTCAATGACTTTCTTCTCAAGTTGAAGGACAACCTAGAGCTAGTGCAGGAGGAGAAGGATCAGAAAGTCTCCACCAAAAACGCCGTCGTCGAGTCCAGGGCTGTCACTCCCACTTGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01110 map00941 map01110
Pfam Domains
Protein Families
NmrA

Protein Analysis

423

Amino Acids

47.01

Weight (kDa)

9.33

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 83 - 381 8.3e-53 NmrA-like family
Epimerase PF01370 85 - 170 1.9e-06 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 89 - 235 1.4e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016318)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g28110
malus_domestica MD13G1046900.v1.1 MD16G1048500.v1.1
prunus_persica Prupe.1G305100_v2.0.a1
pyrus_communis pycom13g04100 pycom16g04240
rosa_chinensis RchiOBHm_Chr4g0435881
rosa_laevigata RLG00000006553
rosa_multiflora Rmu_sc0003165.1_g000010
rosa_roxburghii Rroxscaffold_5G00377050
rosa_rugosa Rorug04G0290200
rosa_samantha Rh4BG353500 Rh4CG367900 Rh4DG347300
rosa_wichuraiana Rw4G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 266, 670, 782
AccI GTMKAC 2 cut(s) 804, 1006
AccII CGCG 1 cut(s) 842
AciI CCGC 4 cut(s) 312, 492, 840, 884
AclWI GGATC 3 cut(s) 593, 765, 1212
AcoI YGGCCR 2 cut(s) 316, 940
AcsI RAATTY 2 cut(s) 824, 1066
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 2 cut(s) 547, 811
AfiI CCNNNNNNNGG 4 cut(s) 364, 526, 739, 754
AgeI ACCGGT 1 cut(s) 272
AgsI TTSAA 5 cut(s) 126, 388, 608, 1150, 1171
AhdI GACNNNNNGTC 2 cut(s) 164, 653
AjnI CCWGG 4 cut(s) 343, 610, 1114, 1241
AluBI AGCT 2 cut(s) 196, 1186
AluI AGCT 2 cut(s) 196, 1186
Alw26I GTCTC 2 cut(s) 464, 1219
AlwI GGATC 3 cut(s) 593, 765, 1212
AoxI GGCC 7 cut(s) 316, 341, 346, 528, 710, 940, 1082
ApeKI GCWGC 6 cut(s) 200, 203, 206, 536, 690, 992
ApoI RAATTY 2 cut(s) 824, 1066
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
AsiGI ACCGGT 1 cut(s) 272
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 5 cut(s) 347, 508, 711, 1082, 1131
AsuHPI GGTGA 6 cut(s) 213, 347, 610, 788, 952, 1075
AsuNHI GCTAGC 1 cut(s) 196
AvaII GGWCC 2 cut(s) 508, 1131
BaeGI GKGCMC 1 cut(s) 675
BanI GGYRCC 3 cut(s) 266, 670, 782
BanII GRGCYC 1 cut(s) 618
BauI CACGAG 1 cut(s) 1086
BbsI GAAGAC 1 cut(s) 163
BbvI GCAGC 6 cut(s) 212, 215, 218, 548, 677, 979
BccI CCATC 4 cut(s) 40, 539, 773, 971
BceAI ACGGC 2 cut(s) 470, 1214
BciT130I CCWGG 4 cut(s) 345, 612, 1116, 1243
BciVI GTATCC 1 cut(s) 39
BcoDI GTCTC 2 cut(s) 464, 1219
BfaI CTAG 6 cut(s) 197, 521, 954, 983, 1181, 1187
BfmI CTRYAG 2 cut(s) 178, 993
BfoI RGCGCY 1 cut(s) 402
BfuI GTATCC 1 cut(s) 39
BisI GCNGC 7 cut(s) 201, 204, 207, 312, 537, 691, 993
BlsI GCNGC 7 cut(s) 202, 205, 208, 313, 538, 692, 994
Bme1390I CCNGG 4 cut(s) 345, 612, 1116, 1243
Bme18I GGWCC 2 cut(s) 508, 1131
BmeRI GACNNNNNGTC 2 cut(s) 164, 653
BmgT120I GGNCC 5 cut(s) 347, 508, 711, 1082, 1131
BmiI GGNNCC 5 cut(s) 268, 672, 764, 784, 1083
BmrFI CCNGG 4 cut(s) 345, 612, 1116, 1243
BmsI GCATC 2 cut(s) 926, 1034
BmtI GCTAGC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 163
BpmI CTGGAG 1 cut(s) 1137
BpuEI CTTGAG 2 cut(s) 151, 1148
BsaAI YACGTR 1 cut(s) 327
BsaJI CCNNGG 4 cut(s) 611, 1114, 1134, 1242
BsaWI WCCGGW 2 cut(s) 272, 601
Bsc4I CCNNNNNNNGG 4 cut(s) 364, 526, 739, 754
Bse118I RCCGGY 2 cut(s) 272, 314
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 4 cut(s) 345, 612, 1116, 1243
BseDI CCNNGG 4 cut(s) 611, 1114, 1134, 1242
BseGI GGATG 3 cut(s) 849, 1061, 1144
BseLI CCNNNNNNNGG 4 cut(s) 364, 526, 739, 754
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 2 cut(s) 443, 729
BseRI GAGGAG 1 cut(s) 1211
BseSI GKGCMC 1 cut(s) 675
BseXI GCAGC 6 cut(s) 212, 215, 218, 548, 677, 979
BsgI GTGCAG 1 cut(s) 1211
Bsh1236I CGCG 1 cut(s) 842
Bsh1285I CGRYCG 1 cut(s) 884
BshFI GGCC 7 cut(s) 318, 343, 348, 530, 712, 942, 1084
BshNI GGYRCC 3 cut(s) 266, 670, 782
BshTI ACCGGT 1 cut(s) 272
BsiEI CGRYCG 1 cut(s) 884
BsiSI CCGG 5 cut(s) 273, 315, 340, 602, 943
BslI CCNNNNNNNGG 4 cut(s) 364, 526, 739, 754
BsmAI GTCTC 2 cut(s) 464, 1219
BsnI GGCC 7 cut(s) 318, 343, 348, 530, 712, 942, 1084
Bsp1286I GDGCHC 2 cut(s) 618, 675
Bsp143I GATC 3 cut(s) 598, 757, 1204
BspACI CCGC 4 cut(s) 312, 492, 840, 884
BspANI GGCC 7 cut(s) 318, 343, 348, 530, 712, 942, 1084
BspCNI CTCAG 2 cut(s) 444, 730
BspFNI CGCG 1 cut(s) 842
BspLI GGNNCC 5 cut(s) 268, 672, 764, 784, 1083
BspMAI CTGCAG 1 cut(s) 997
BspOI GCTAGC 1 cut(s) 200
BspPI GGATC 3 cut(s) 593, 765, 1212
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 3 cut(s) 266, 670, 782
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 2 cut(s) 272, 314
BssAI RCCGGY 2 cut(s) 272, 314
BssECI CCNNGG 4 cut(s) 611, 1114, 1134, 1242
BssMI GATC 3 cut(s) 598, 757, 1204
BssNAI GTATAC 1 cut(s) 1007
BssSI CACGAG 1 cut(s) 1086
BssT1I CCWWGG 1 cut(s) 1134
Bst1107I GTATAC 1 cut(s) 1007
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 4 cut(s) 345, 612, 1116, 1243
Bst4CI ACNGT 8 cut(s) 217, 376, 475, 550, 753, 776, 787, 809
Bst6I CTCTTC 2 cut(s) 186, 357
BstBAI YACGTR 1 cut(s) 327
BstC8I GCNNGC 4 cut(s) 198, 304, 316, 915
BstDEI CTNAG 3 cut(s) 363, 452, 738
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 3 cut(s) 849, 1061, 1144
BstFNI CGCG 1 cut(s) 842
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 3 cut(s) 601, 760, 1207
BstMAI GTCTC 2 cut(s) 464, 1219
BstMBI GATC 3 cut(s) 598, 757, 1204
BstMCI CGRYCG 1 cut(s) 884
BstMWI GCNNNNNNNGC 4 cut(s) 206, 308, 536, 992
BstNI CCWGG 4 cut(s) 345, 612, 1116, 1243
BstNSI RCATGY 1 cut(s) 84
BstSCI CCNGG 4 cut(s) 343, 610, 1114, 1241
BstSFI CTRYAG 2 cut(s) 178, 993
BstSLI GKGCMC 1 cut(s) 675
BstUI CGCG 1 cut(s) 842
BstV1I GCAGC 6 cut(s) 212, 215, 218, 548, 677, 979
BstV2I GAAGAC 1 cut(s) 163
BstXI CCANNNNNNTGG 1 cut(s) 628
BstZ17I GTATAC 1 cut(s) 1007
BsuI GTATCC 1 cut(s) 39
BsuRI GGCC 7 cut(s) 318, 343, 348, 530, 712, 942, 1084
BtgZI GCGATG 1 cut(s) 517
BtsCI GGATG 3 cut(s) 849, 1061, 1144
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 2 cut(s) 966, 1080
Cac8I GCNNGC 4 cut(s) 198, 304, 316, 915
CfoI GCGC 1 cut(s) 401
Cfr10I RCCGGY 2 cut(s) 272, 314
Cfr13I GGNCC 5 cut(s) 347, 508, 711, 1082, 1131
Csp6I GTAC 2 cut(s) 546, 810
CspAI ACCGGT 1 cut(s) 272
CviAII CATG 6 cut(s) 81, 211, 461, 581, 622, 1037
CviQI GTAC 2 cut(s) 546, 810
DdeI CTNAG 3 cut(s) 363, 452, 738
DpnI GATC 3 cut(s) 600, 759, 1206
DpnII GATC 3 cut(s) 598, 757, 1204
DriI GACNNNNNGTC 2 cut(s) 164, 653
EaeI YGGCCR 2 cut(s) 316, 940
Eam1104I CTCTTC 2 cut(s) 186, 357
Eam1105I GACNNNNNGTC 2 cut(s) 164, 653
EarI CTCTTC 2 cut(s) 186, 357
Eco130I CCWWGG 1 cut(s) 1134
Eco24I GRGCYC 1 cut(s) 618
Eco47I GGWCC 2 cut(s) 508, 1131
Eco57I CTGAAG 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoO109I RGGNCCY 2 cut(s) 1082, 1131
EcoRII CCWGG 4 cut(s) 343, 610, 1114, 1241
EcoT14I CCWWGG 1 cut(s) 1134
EcoT38I GRGCYC 1 cut(s) 618
ErhI CCWWGG 1 cut(s) 1134
FaeI CATG 6 cut(s) 84, 214, 464, 584, 625, 1040
FatI CATG 6 cut(s) 80, 210, 460, 580, 621, 1036
FblI GTMKAC 2 cut(s) 804, 1006
Fnu4HI GCNGC 7 cut(s) 201, 204, 207, 312, 537, 691, 993
FokI GGATG 3 cut(s) 836, 1068, 1151
FriOI GRGCYC 1 cut(s) 618
Fsp4HI GCNGC 7 cut(s) 201, 204, 207, 312, 537, 691, 993
FspBI CTAG 6 cut(s) 197, 521, 954, 983, 1181, 1187
GlaI GCGC 1 cut(s) 400
GluI GCNGC 7 cut(s) 201, 204, 207, 312, 537, 691, 993
GsuI CTGGAG 1 cut(s) 1137
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 7 cut(s) 318, 343, 348, 530, 712, 942, 1084
HapII CCGG 5 cut(s) 273, 315, 340, 602, 943
HhaI GCGC 1 cut(s) 401
Hin1II CATG 6 cut(s) 84, 214, 464, 584, 625, 1040
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HinfI GANTC 6 cut(s) 252, 946, 957, 1000, 1121, 1238
HpaII CCGG 5 cut(s) 273, 315, 340, 602, 943
HphI GGTGA 6 cut(s) 213, 347, 610, 788, 952, 1075
Hpy166II GTNNAC 4 cut(s) 589, 805, 1007, 1032
Hpy188I TCNGA 6 cut(s) 427, 571, 739, 854, 881, 1209
Hpy188III TCNNGA 5 cut(s) 283, 469, 658, 1088, 1129
Hpy8I GTNNAC 4 cut(s) 589, 805, 1007, 1032
Hpy99I CGWCG 3 cut(s) 491, 1235, 1238
HpyAV CCTTC 9 cut(s) 181, 234, 369, 394, 576, 744, 838, 1165, 1195
HpyCH4III ACNGT 8 cut(s) 217, 376, 475, 550, 753, 776, 787, 809
HpyCH4IV ACGT 2 cut(s) 326, 649
HpyCH4V TGCA 5 cut(s) 693, 917, 995, 1073, 1192
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 308, 536, 992
HpyF3I CTNAG 3 cut(s) 363, 452, 738
HpySE526I ACGT 2 cut(s) 326, 649
Hsp92II CATG 6 cut(s) 84, 214, 464, 584, 625, 1040
HspAI GCGC 1 cut(s) 399
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Kzo9I GATC 3 cut(s) 598, 757, 1204
LguI GCTCTTC 1 cut(s) 186
Lsp1109I GCAGC 6 cut(s) 212, 215, 218, 548, 677, 979
LweI GCATC 2 cut(s) 926, 1034
MaeI CTAG 6 cut(s) 197, 521, 954, 983, 1181, 1187
MaeII ACGT 2 cut(s) 326, 649
MaeIII GTNAC 2 cut(s) 13, 1249
MalI GATC 3 cut(s) 600, 759, 1206
MboI GATC 3 cut(s) 598, 757, 1204
MboII GAAGA 6 cut(s) 168, 203, 344, 457, 943, 1151
MhlI GDGCHC 2 cut(s) 618, 675
MluCI AATT 4 cut(s) 824, 892, 936, 1066
MlyI GAGTC 3 cut(s) 261, 940, 1247
MmeI TCCRAC 2 cut(s) 735, 904
MroNI GCCGGC 1 cut(s) 314
MseI TTAA 1 cut(s) 552
MslI CAYNNNNRTG 2 cut(s) 626, 922
MspI CCGG 5 cut(s) 273, 315, 340, 602, 943
MspR9I CCNGG 4 cut(s) 345, 612, 1116, 1243
MvaI CCWGG 4 cut(s) 345, 612, 1116, 1243
MvnI CGCG 1 cut(s) 842
MwoI GCNNNNNNNGC 4 cut(s) 206, 308, 536, 992
NaeI GCCGGC 1 cut(s) 316
NdeII GATC 3 cut(s) 598, 757, 1204
NgoMIV GCCGGC 1 cut(s) 314
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 6 cut(s) 84, 214, 464, 584, 625, 1040
NlaIV GGNNCC 5 cut(s) 268, 672, 764, 784, 1083
NmeAIII GCCGAG 1 cut(s) 912
NmuCI GTSAC 1 cut(s) 1249
NspI RCATGY 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 1233
PdiI GCCGGC 1 cut(s) 316
PfeI GAWTC 3 cut(s) 957, 1000, 1121
PflFI GACNNNGTC 1 cut(s) 374
PinAI ACCGGT 1 cut(s) 272
PkrI GCNGC 7 cut(s) 202, 205, 208, 313, 538, 692, 994
PleI GAGTC 3 cut(s) 260, 940, 1246
PpsI GAGTC 3 cut(s) 260, 940, 1246
Ppu21I YACGTR 1 cut(s) 327
PpuMI RGGWCCY 1 cut(s) 1131
Psp5II RGGWCCY 1 cut(s) 1131
Psp6I CCWGG 4 cut(s) 343, 610, 1114, 1241
PspGI CCWGG 4 cut(s) 343, 610, 1114, 1241
PspN4I GGNNCC 5 cut(s) 268, 672, 764, 784, 1083
PspPI GGNCC 5 cut(s) 347, 508, 711, 1082, 1131
PspPPI RGGWCCY 1 cut(s) 1131
PstI CTGCAG 1 cut(s) 997
PsyI GACNNNGTC 1 cut(s) 374
RsaI GTAC 2 cut(s) 547, 811
RsaNI GTAC 2 cut(s) 546, 810
RseI CAYNNNNRTG 2 cut(s) 626, 922
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 7 cut(s) 201, 204, 207, 312, 537, 691, 993
Sau3AI GATC 3 cut(s) 598, 757, 1204
Sau96I GGNCC 5 cut(s) 347, 508, 711, 1082, 1131
SchI GAGTC 3 cut(s) 261, 940, 1247
ScrFI CCNGG 4 cut(s) 345, 612, 1116, 1243
SduI GDGCHC 2 cut(s) 618, 675
SfaNI GCATC 2 cut(s) 926, 1034
SfcI CTRYAG 2 cut(s) 178, 993
SinI GGWCC 2 cut(s) 508, 1131
SmiMI CAYNNNNRTG 2 cut(s) 626, 922
SmlI CTYRAG 2 cut(s) 130, 1163
SmoI CTYRAG 2 cut(s) 130, 1163
Sse9I AATT 4 cut(s) 824, 892, 936, 1066
SsiI CCGC 4 cut(s) 312, 492, 840, 884
SspI AATATT 1 cut(s) 504
SspMI CTAG 6 cut(s) 197, 521, 954, 983, 1181, 1187
StyD4I CCNGG 4 cut(s) 343, 610, 1114, 1241
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 8 cut(s) 217, 376, 475, 550, 753, 776, 787, 809
TaiI ACGT 2 cut(s) 329, 652
TaqI TCGA 4 cut(s) 468, 525, 657, 1236
TasI AATT 4 cut(s) 824, 892, 936, 1066
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 3 cut(s) 957, 1000, 1121
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 2 cut(s) 973, 1080
TseFI GTSAC 1 cut(s) 1249
TseI GCWGC 6 cut(s) 200, 203, 206, 536, 690, 992
Tsp45I GTSAC 1 cut(s) 1249
TspDTI ATGAA 3 cut(s) 268, 477, 863
TspGWI ACGGA 1 cut(s) 827
TspRI CASTG 2 cut(s) 973, 1080
Tth111I GACNNNGTC 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 508, 1131
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 2 cut(s) 824, 1066
XceI RCATGY 1 cut(s) 84
XmiI GTMKAC 2 cut(s) 804, 1006
XspI CTAG 6 cut(s) 197, 521, 954, 983, 1181, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.