MD13G1048600.v1.1

Piriformospora indica-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012746
Physical Location & Seq
Forward (+)
3404565 .. 3406236
1672 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1048600.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGAAGGGTTTTACTCCCATCAGTGTTGTGATTTTGGTAACCTTTCTGTTCTCTGTGGGTGCTTGGAGTTCTGAGGAAGTAGATGAAGGTGTAGCTCCAATGGAGAAAACGGAGCAATTCGCTCTGTACTCTGCAATCCAAGGCTTTGTGGGTAAATGGTGGAATGGCTCAGACCTCTATCCGGATCCTTGTGGGTGGACTCCTATACAGGGGGTGTCTTGTGATTTGTTTGATGGGGTTTGGTACGTGACCTCCATGAATATTGGACCTATTCTTGACAACTCCCTTGGTTGTTCCCCAAGCGCAAAATTTAGACCACAGCTGTTTGATTTGAAGCACCTAAAGTCCCTGTCCATTTTCAATTGCTTCCTCTCACCCCACAAGCATCCAGTTCCGATCCCCTCAGAAAACTGGTGGAAGGTTGCCGGCAGCTTAGAATCACTAGAGTTTCGATCGAACCCGGGCCTCATTGGACAGATTCCTACAACCTTTGGCAGCCTCAGAAAGCTCCAATCATTGGTACTAGTGGAGAATGGATTAAGAGGTGGATTACCAACAAATATCGGTGAATTAGTCCGATTGAAGCGGCTAGTTCTTACCGGAAACTGGTTTACGGGCCAAATCCCCGATGGGTTTGGTGAATTGAATCAGCTGCTGATCCTTGATTTAAGTAGGAACTCACTGTCTGGGCCTTTGCCAATCACCATTGGACGTTTGACTTCACTCTTGAAGCTTGACTTGAGCAGCAATCAACTGGAAGGGCAGCTGCCAATAGGTTTTGGTAATCTAAAGAAGTTGACTTTATTGGACCTCAGGAGCAACAATTTCTCGGGTGGATTGACCAAATCACTTCAAGAAATGCATTCCTTGGAGGAAATGGTTTTATCCAACAACCCAATTGGTGGAGACCTCAAAACCCTAGAGTGGCAAAACATGGAGAGTTTGGTATTTTTAGACCTCTCCGGATTAGGTCTAATTGGTGAGATTCCAGATTCATTTTCAAACCTAAAAAAGTTGAGATTTTTGGGTTTAAGTGACAACAAGCTCACAGGCAACCTCTTGCCAAAGATTGCAACTTTGCCCTGCCTCAGTGCACTCTACCTCCACGGCAACAACCTGACAGGGGAGCTTAAATTCTCTGAGTCGTTTTATCACAAAATGGGGAATCGTTTTGGTGCTTGGAACAACCCTAATCTCTGCTACACCAGTGGAATGGTGCCAGCAGAGCATGTCCCAATTGGGGTGAGGCCATGTCAGCAGGAGGGGGAGGAGGTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8

Protein Analysis

427

Amino Acids

46.81

Weight (kDa)

5.72

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 164 - 384 9.4e-16 Leucine-rich repeat region
LRR_8 PF13855 193 - 252 1.1e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13230 AT3G25670
fragaria_vesca FvH4_4g28250
malus_domestica MD13G1048600.v1.1 MD16G1049900.v1.1
prunus_persica Prupe.1G303600_v2.0.a1
pyrus_communis pycom13g04290 pycom16g04360
rosa_chinensis RchiOBHm_Chr4g0436111
rosa_laevigata RLG00000006538
rosa_multiflora Rmu_sc0006301.1_g000003
rosa_roxburghii Rroxscaffold_5G00377220
rosa_rugosa Rorug04G0292100
rosa_samantha Rh4AG346100 Rh4BG355200 Rh4CG369800 Rh4DG348800
rosa_wichuraiana Rw4G030250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1216
AccB7I CCANNNNNTGG 2 cut(s) 517, 902
AccIII TCCGGA 2 cut(s) 181, 962
AciI CCGC 1 cut(s) 586
AclWI GGATC 4 cut(s) 179, 192, 391, 652
AcsI RAATTY 2 cut(s) 308, 1133
AfaI GTAC 3 cut(s) 128, 245, 522
AfiI CCNNNNNNNGG 8 cut(s) 181, 209, 517, 606, 902, 924, 1123, 1240
AgsI TTSAA 7 cut(s) 334, 361, 583, 646, 730, 854, 1002
AhlI ACTAGT 1 cut(s) 523
AluBI AGCT 9 cut(s) 95, 322, 432, 508, 652, 733, 766, 1045, 1129
AluI AGCT 9 cut(s) 95, 322, 432, 508, 652, 733, 766, 1045, 1129
Alw21I GWGCWC 1 cut(s) 1096
Alw26I GTCTC 1 cut(s) 900
Alw44I GTGCAC 1 cut(s) 1092
AlwI GGATC 4 cut(s) 179, 192, 391, 652
AlwNI CAGNNNCTG 1 cut(s) 655
Ama87I CYCGRG 2 cut(s) 460, 829
Aor13HI TCCGGA 2 cut(s) 181, 962
AoxI GGCC 4 cut(s) 463, 616, 689, 1247
ApaLI GTGCAC 1 cut(s) 1092
ApeKI GCWGC 6 cut(s) 429, 495, 652, 744, 763, 766
ApoI RAATTY 2 cut(s) 308, 1133
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 5 cut(s) 266, 463, 616, 689, 808
AsuC2I CCSGG 2 cut(s) 461, 462
AsuHPI GGTGA 6 cut(s) 366, 578, 650, 694, 992, 1255
AvaI CYCGRG 2 cut(s) 460, 829
AvaII GGWCC 2 cut(s) 266, 808
AxyI CCTNAGG 1 cut(s) 812
BaeGI GKGCMC 1 cut(s) 1096
BamHI GGATCC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 1216
Bbv12I GWGCWC 1 cut(s) 1096
BbvI GCAGC 6 cut(s) 441, 507, 639, 753, 756, 775
BccI CCATC 3 cut(s) 26, 227, 623
BceAI ACGGC 1 cut(s) 1123
BcnI CCSGG 2 cut(s) 461, 462
BcoDI GTCTC 1 cut(s) 900
BcuI ACTAGT 1 cut(s) 523
BfaI CTAG 4 cut(s) 443, 524, 590, 920
BisI GCNGC 7 cut(s) 430, 496, 587, 653, 745, 764, 767
BlsI GCNGC 7 cut(s) 431, 497, 588, 654, 746, 765, 768
Bme1390I CCNGG 2 cut(s) 461, 462
Bme18I GGWCC 2 cut(s) 266, 808
BmeT110I CYCGRG 2 cut(s) 460, 829
BmgT120I GGNCC 5 cut(s) 266, 463, 616, 689, 808
BmiI GGNNCC 2 cut(s) 186, 1218
BmrFI CCNGG 2 cut(s) 461, 462
BmsI GCATC 1 cut(s) 394
BpuEI CTTGAG 1 cut(s) 760
BpuMI CCSGG 2 cut(s) 461, 462
BsaAI YACGTR 1 cut(s) 247
BsaI GGTCTC 1 cut(s) 900
BsaJI CCNNGG 5 cut(s) 139, 286, 460, 867, 1105
BsaWI WCCGGW 3 cut(s) 181, 599, 962
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 1086, 1116
Bsc4I CCNNNNNNNGG 8 cut(s) 181, 209, 517, 606, 902, 924, 1123, 1240
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 5 cut(s) 389, 416, 611, 759, 1206
Bse21I CCTNAGG 1 cut(s) 812
BseAI TCCGGA 2 cut(s) 181, 962
BseDI CCNNGG 5 cut(s) 139, 286, 460, 867, 1105
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 8 cut(s) 181, 209, 517, 606, 902, 924, 1123, 1240
BseMII CTCAG 7 cut(s) 63, 183, 417, 514, 826, 1102, 1131
BseNI ACTGG 5 cut(s) 389, 416, 611, 759, 1206
BseSI GKGCMC 1 cut(s) 1096
BseXI GCAGC 6 cut(s) 441, 507, 639, 753, 756, 775
Bsh1285I CGRYCG 1 cut(s) 455
BshFI GGCC 4 cut(s) 465, 618, 691, 1249
BshNI GGYRCC 1 cut(s) 1216
BsiEI CGRYCG 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 1096
BsiHKCI CYCGRG 2 cut(s) 460, 829
BsiSI CCGG 5 cut(s) 182, 426, 461, 600, 963
BslFI GGGAC 2 cut(s) 331, 1217
BslI CCNNNNNNNGG 8 cut(s) 181, 209, 517, 606, 902, 924, 1123, 1240
BsmAI GTCTC 1 cut(s) 900
BsmFI GGGAC 2 cut(s) 331, 1217
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 4 cut(s) 465, 618, 691, 1249
Bso31I GGTCTC 1 cut(s) 900
BsoBI CYCGRG 2 cut(s) 460, 829
Bsp1286I GDGCHC 1 cut(s) 1096
Bsp13I TCCGGA 2 cut(s) 181, 962
Bsp143I GATC 4 cut(s) 184, 396, 452, 657
BspACI CCGC 1 cut(s) 586
BspANI GGCC 4 cut(s) 465, 618, 691, 1249
BspCNI CTCAG 7 cut(s) 64, 182, 416, 513, 825, 1101, 1132
BspEI TCCGGA 2 cut(s) 181, 962
BspLI GGNNCC 2 cut(s) 186, 1218
BspPI GGATC 4 cut(s) 179, 192, 391, 652
BspT107I GGYRCC 1 cut(s) 1216
BspTNI GGTCTC 1 cut(s) 900
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 5 cut(s) 389, 416, 611, 759, 1206
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 5 cut(s) 139, 286, 460, 867, 1105
BssMI GATC 4 cut(s) 184, 396, 452, 657
BssT1I CCWWGG 3 cut(s) 139, 286, 867
Bst4CI ACNGT 1 cut(s) 684
BstBAI YACGTR 1 cut(s) 247
BstC8I GCNNGC 2 cut(s) 427, 1221
BstDEI CTNAG 8 cut(s) 72, 169, 403, 433, 500, 812, 1088, 1140
BstDSI CCRYGG 1 cut(s) 1105
BstEII GGTNACC 1 cut(s) 37
BstF5I GGATG 1 cut(s) 385
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 4 cut(s) 187, 399, 455, 660
BstMAI GTCTC 1 cut(s) 900
BstMBI GATC 4 cut(s) 184, 396, 452, 657
BstMCI CGRYCG 1 cut(s) 455
BstMWI GCNNNNNNNGC 2 cut(s) 1225, 1255
BstNSI RCATGY 1 cut(s) 1232
BstPI GGTNACC 1 cut(s) 37
BstSCI CCNGG 2 cut(s) 459, 460
BstSLI GKGCMC 1 cut(s) 1096
BstV1I GCAGC 6 cut(s) 441, 507, 639, 753, 756, 775
BstX2I RGATCY 1 cut(s) 184
BstXI CCANNNNNNTGG 1 cut(s) 1213
BstYI RGATCY 1 cut(s) 184
Bsu36I CCTNAGG 1 cut(s) 812
BsuRI GGCC 4 cut(s) 465, 618, 691, 1249
BtgI CCRYGG 1 cut(s) 1105
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 4 cut(s) 28, 680, 1096, 1213
Cac8I GCNNGC 2 cut(s) 427, 1221
CaiI CAGNNNCTG 1 cut(s) 655
CfoI GCGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 5 cut(s) 266, 463, 616, 689, 808
Cfr9I CCCGGG 1 cut(s) 460
Csp6I GTAC 3 cut(s) 127, 244, 521
CviAII CATG 4 cut(s) 256, 934, 1229, 1251
CviQI GTAC 3 cut(s) 127, 244, 521
DdeI CTNAG 8 cut(s) 72, 169, 403, 433, 500, 812, 1088, 1140
DpnI GATC 4 cut(s) 186, 398, 454, 659
DpnII GATC 4 cut(s) 184, 396, 452, 657
Eco130I CCWWGG 3 cut(s) 139, 286, 867
Eco31I GGTCTC 1 cut(s) 900
Eco47I GGWCC 2 cut(s) 266, 808
Eco81I CCTNAGG 1 cut(s) 812
Eco88I CYCGRG 2 cut(s) 460, 829
Eco91I GGTNACC 1 cut(s) 37
EcoO65I GGTNACC 1 cut(s) 37
EcoT14I CCWWGG 3 cut(s) 139, 286, 867
EcoT22I ATGCAT 1 cut(s) 864
ErhI CCWWGG 3 cut(s) 139, 286, 867
FaeI CATG 4 cut(s) 259, 937, 1232, 1254
FaiI YATR 5 cut(s) 206, 257, 935, 1230, 1252
FalI AAGNNNNNCTT 2 cut(s) 722, 754
FaqI GGGAC 2 cut(s) 331, 1217
FatI CATG 4 cut(s) 255, 933, 1228, 1250
Fnu4HI GCNGC 7 cut(s) 430, 496, 587, 653, 745, 764, 767
FokI GGATG 1 cut(s) 372
Fsp4HI GCNGC 7 cut(s) 430, 496, 587, 653, 745, 764, 767
FspBI CTAG 4 cut(s) 443, 524, 590, 920
GlaI GCGC 1 cut(s) 304
GluI GCNGC 7 cut(s) 430, 496, 587, 653, 745, 764, 767
HaeIII GGCC 4 cut(s) 465, 618, 691, 1249
HapII CCGG 5 cut(s) 182, 426, 461, 600, 963
HhaI GCGC 1 cut(s) 305
Hin1II CATG 4 cut(s) 259, 937, 1232, 1254
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 8 cut(s) 199, 437, 478, 646, 985, 992, 1142, 1165
HpaII CCGG 5 cut(s) 182, 426, 461, 600, 963
HphI GGTGA 6 cut(s) 366, 578, 650, 694, 992, 1255
Hpy166II GTNNAC 4 cut(s) 198, 612, 798, 1094
Hpy188I TCNGA 7 cut(s) 73, 172, 396, 406, 503, 578, 1141
Hpy188III TCNNGA 7 cut(s) 182, 275, 727, 814, 854, 963, 989
Hpy8I GTNNAC 4 cut(s) 198, 612, 798, 1094
HpyAV CCTTC 3 cut(s) 80, 412, 752
HpyCH4III ACNGT 1 cut(s) 684
HpyCH4IV ACGT 2 cut(s) 246, 712
HpyCH4V TGCA 4 cut(s) 134, 862, 1073, 1094
HpyF10VI GCNNNNNNNGC 2 cut(s) 1225, 1255
HpyF3I CTNAG 8 cut(s) 72, 169, 403, 433, 500, 812, 1088, 1140
HpySE526I ACGT 2 cut(s) 246, 712
Hsp92II CATG 4 cut(s) 259, 937, 1232, 1254
HspAI GCGC 1 cut(s) 303
Kpn2I TCCGGA 2 cut(s) 181, 962
KroI GCCGGC 1 cut(s) 425
KroNI GCCGGC 1 cut(s) 427
Kzo9I GATC 4 cut(s) 184, 396, 452, 657
LmnI GCTCC 5 cut(s) 100, 112, 513, 816, 1126
Lsp1109I GCAGC 6 cut(s) 441, 507, 639, 753, 756, 775
LweI GCATC 1 cut(s) 394
MaeI CTAG 4 cut(s) 443, 524, 590, 920
MaeII ACGT 2 cut(s) 246, 712
MaeIII GTNAC 4 cut(s) 37, 247, 1034, 1273
MalI GATC 4 cut(s) 186, 398, 454, 659
MboI GATC 4 cut(s) 184, 396, 452, 657
MfeI CAATTG 3 cut(s) 361, 897, 1236
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 1096
MlyI GAGTC 2 cut(s) 193, 1151
MmeI TCCRAC 1 cut(s) 912
Mph1103I ATGCAT 1 cut(s) 864
MroI TCCGGA 2 cut(s) 181, 962
MroNI GCCGGC 1 cut(s) 425
MseI TTAA 4 cut(s) 539, 668, 1031, 1131
MspA1I CMGCKG 3 cut(s) 322, 652, 766
MspI CCGG 5 cut(s) 182, 426, 461, 600, 963
MspR9I CCNGG 2 cut(s) 461, 462
MunI CAATTG 3 cut(s) 361, 897, 1236
Mva1269I GAATGC 1 cut(s) 862
MwoI GCNNNNNNNGC 2 cut(s) 1225, 1255
NaeI GCCGGC 1 cut(s) 427
NciI CCSGG 2 cut(s) 461, 462
NdeII GATC 4 cut(s) 184, 396, 452, 657
NgoMIV GCCGGC 1 cut(s) 425
NlaIII CATG 4 cut(s) 259, 937, 1232, 1254
NlaIV GGNNCC 2 cut(s) 186, 1218
NmuCI GTSAC 3 cut(s) 247, 1034, 1273
NsiI ATGCAT 1 cut(s) 864
NspI RCATGY 1 cut(s) 1232
PctI GAATGC 1 cut(s) 862
PdiI GCCGGC 1 cut(s) 427
PfeI GAWTC 6 cut(s) 437, 478, 646, 985, 992, 1165
PflMI CCANNNNNTGG 2 cut(s) 517, 902
PkrI GCNGC 7 cut(s) 431, 497, 588, 654, 746, 765, 768
Ple19I CGATCG 1 cut(s) 455
PleI GAGTC 2 cut(s) 193, 1150
PpsI GAGTC 2 cut(s) 193, 1150
Ppu21I YACGTR 1 cut(s) 247
PspEI GGTNACC 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 186, 1218
PspPI GGNCC 5 cut(s) 266, 463, 616, 689, 808
PstNI CAGNNNCTG 1 cut(s) 655
PsuI RGATCY 1 cut(s) 184
PvuI CGATCG 1 cut(s) 455
PvuII CAGCTG 3 cut(s) 322, 652, 766
RsaI GTAC 3 cut(s) 128, 245, 522
RsaNI GTAC 3 cut(s) 127, 244, 521
SaqAI TTAA 4 cut(s) 539, 668, 1031, 1131
SatI GCNGC 7 cut(s) 430, 496, 587, 653, 745, 764, 767
Sau3AI GATC 4 cut(s) 184, 396, 452, 657
Sau96I GGNCC 5 cut(s) 266, 463, 616, 689, 808
SchI GAGTC 2 cut(s) 193, 1151
ScrFI CCNGG 2 cut(s) 461, 462
SduI GDGCHC 1 cut(s) 1096
SfaNI GCATC 1 cut(s) 394
SinI GGWCC 2 cut(s) 266, 808
SmaI CCCGGG 1 cut(s) 462
SmlI CTYRAG 1 cut(s) 739
SmoI CTYRAG 1 cut(s) 739
SpeI ACTAGT 1 cut(s) 523
SsiI CCGC 1 cut(s) 586
SspI AATATT 1 cut(s) 262
SspMI CTAG 4 cut(s) 443, 524, 590, 920
StyD4I CCNGG 2 cut(s) 459, 460
StyI CCWWGG 3 cut(s) 139, 286, 867
TaaI ACNGT 1 cut(s) 684
TaiI ACGT 2 cut(s) 249, 715
TaqI TCGA 2 cut(s) 451, 455
TatI WGTACW 1 cut(s) 126
TauI GCSGC 1 cut(s) 589
TfiI GAWTC 6 cut(s) 437, 478, 646, 985, 992, 1165
Tru1I TTAA 4 cut(s) 539, 668, 1031, 1131
Tru9I TTAA 4 cut(s) 539, 668, 1031, 1131
TscAI CASTG 4 cut(s) 28, 687, 1096, 1213
TseFI GTSAC 3 cut(s) 247, 1034, 1273
TseI GCWGC 6 cut(s) 429, 495, 652, 744, 763, 766
Tsp45I GTSAC 3 cut(s) 247, 1034, 1273
TspDTI ATGAA 4 cut(s) 17, 99, 272, 984
TspGWI ACGGA 1 cut(s) 125
TspMI CCCGGG 1 cut(s) 460
TspRI CASTG 4 cut(s) 28, 687, 1096, 1213
Van91I CCANNNNNTGG 2 cut(s) 517, 902
VneI GTGCAC 1 cut(s) 1092
VpaK11BI GGWCC 2 cut(s) 266, 808
XapI RAATTY 2 cut(s) 308, 1133
XceI RCATGY 1 cut(s) 1232
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XmaI CCCGGG 1 cut(s) 460
XspI CTAG 4 cut(s) 443, 524, 590, 920
Zsp2I ATGCAT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.