Rw4G030250

regulation of response to stimulus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012746
Physical Location & Seq
Forward (+)
56945516 .. 56947602
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030250.1

Sequence Viewer

Length: 1437 bp
ATGAAAAGCTACACTTCCGTCAGTGTTCTCATTTTCGCTTTCTTCATCCTCTTCTTGGCTGCTTCGTGCTCTGAAGAAGCAATAGAACAAGTTTTAGCTCCAATGGAGAAAACAGAGCAAGAAGCTCTCTACTCTGCTATTCAAGGCTTTGTGGGTAAATGGTGGAATGGTTCAGACCTCTATCCGGATCCTTGTGGATGGACTCCTATTCAGGGGGTGTCTTGTGATCTCTTCAATGGGCTTTGGTATGTGACAGTTATGAACATTGGACCTGTTCATGACAACTCTCTTGGTTGTTCCCCAAATGCAAAATTCAGACCCCAAATGTTTGATTTGAAGCACCTAAAATCCTTGTCCTTTTCCAGTTGTTTCATCTCACCACACAAACATCCAGTTCAAATCCCCACAGAGAATTGGTGGAAGCTCTCTGAGAGCTTAGAATCATTGGAGTTTCGATCGAATCCCGGTCTTACTGGGCAAATTCCCAGTAGCTTTGGTGGCCTCAGAAAGCTCCAATCTTTGGTGCTAGTAGAGTGTGGGTTAAATGGGGGGTTACCAAGTAACATTGGTGACTTGGTACAGTTAAAGAGGCTAGTTCTCTTTGGAAACTGGTTTACAGGTCCAATTCCTGATAGTTTTGGTGAATTAAATCAGCTCTTAATCTTCGATTTGAGCCAAAACTCATTATCTGGGCCTCTGCCAGACACTCTTGGAAGCTTGACTTCACTGTTGAAGATTGATTTGAGCAACAACTTATTAGGAGGGTCAATTTCTCAAGTGGGTGATCTGAAAAACCTGACCCTTTTGGACCTGAGGAACAACAACTTCTCAGGTGGGTTGACCAAGTCAATTCAAGAGATGCATTCCTTGGAAGAGATGGCCTTGTCCAACAACCCAATTGGTGGAGACCTCAATACCATAGAATGGCAAAACATGGATAAATTGGTCATTTTGGATCTCTCTAAAACGGGGTTAACAGGGGAGATTCCAGAGTCCATATCAGAGTTAAAGAGGTTGAGGTTTTTGGGTCTAAATGACAACAAGCTCAGAGGTAACCTCTCACCAAAGCTTGCAACTTTGCCTTGCGTTAGTGCACTTTACCTACATGGGAACAACCTGACAGGGGAGCTTAAATTCTCCGAGCCGTTTTACTCCAAAATGGGTAGTCGTTTTGGTGCTTGGAACAACCCTAATCTCTGCTACGTTGATGCATTGGTCACAAAGAGCAATGTACCATTTGGGGTCAAGCCATGCCGGGCTGAGGTCACATTGCTCGAATCGAATTCAAAAACAAGGCTGGAAGATGGGAGTTTGAATCAAAATTCCAACTTCACTGCTTCTTTGGGATTCTCTAGTCGTGGTGGCTTTGATGGGCTTTGGTGGCTTGGTGGGTTGCTGCAATTAATAATAGTGCTGTTGTTGAATTGGTTCATTTAG

Protein Analysis

478

Amino Acids

52.56

Weight (kDa)

5.4

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 166 - 377 1.6e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13230 AT3G25670
fragaria_vesca FvH4_4g28250
malus_domestica MD13G1048600.v1.1 MD16G1049900.v1.1
prunus_persica Prupe.1G303600_v2.0.a1
pyrus_communis pycom13g04290 pycom16g04360
rosa_chinensis RchiOBHm_Chr4g0436111
rosa_laevigata RLG00000006538
rosa_multiflora Rmu_sc0006301.1_g000003
rosa_roxburghii Rroxscaffold_5G00377220
rosa_rugosa Rorug04G0292100
rosa_samantha Rh4AG346100 Rh4BG355200 Rh4CG369800 Rh4DG348800
rosa_wichuraiana Rw4G030250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 520, 902, 924
AccIII TCCGGA 1 cut(s) 184
AclWI GGATC 3 cut(s) 182, 195, 963
AcsI RAATTY 5 cut(s) 311, 480, 1133, 1282, 1321
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 2 cut(s) 579, 1233
AfiI CCNNNNNNNGG 8 cut(s) 55, 184, 212, 520, 902, 924, 1123, 1261
AgsI TTSAA 9 cut(s) 143, 235, 337, 398, 733, 854, 1287, 1315, 1423
Alw21I GWGCWC 2 cut(s) 71, 1096
Alw26I GTCTC 1 cut(s) 900
Alw44I GTGCAC 1 cut(s) 1092
AlwI GGATC 3 cut(s) 182, 195, 963
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 3 cut(s) 499, 692, 879
ApaLI GTGCAC 1 cut(s) 1092
ApeKI GCWGC 2 cut(s) 59, 1396
ApoI RAATTY 5 cut(s) 311, 480, 1133, 1282, 1321
AseI ATTAAT 1 cut(s) 1403
Asp700I GAANNNNTTC 1 cut(s) 1427
AspS9I GGNCC 4 cut(s) 269, 620, 692, 808
AsuC2I CCSGG 2 cut(s) 465, 1256
AsuHPI GGTGA 5 cut(s) 369, 581, 653, 794, 1053
AvaII GGWCC 3 cut(s) 269, 620, 808
AxyI CCTNAGG 1 cut(s) 812
BaeGI GKGCMC 1 cut(s) 1096
BamHI GGATCC 1 cut(s) 187
Bbv12I GWGCWC 2 cut(s) 71, 1096
BbvCI CCTCAGC 1 cut(s) 1260
BbvI GCAGC 2 cut(s) 46, 1383
BccI CCATC 4 cut(s) 192, 871, 1298, 1364
BceAI ACGGC 1 cut(s) 1129
BcnI CCSGG 2 cut(s) 465, 1256
BcoDI GTCTC 1 cut(s) 900
BfaI CTAG 3 cut(s) 527, 593, 1353
BisI GCNGC 2 cut(s) 60, 1397
BlsI GCNGC 2 cut(s) 61, 1398
Bme1390I CCNGG 2 cut(s) 465, 1256
Bme18I GGWCC 3 cut(s) 269, 620, 808
BmgT120I GGNCC 4 cut(s) 269, 620, 692, 808
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 2 cut(s) 465, 1256
BmrI ACTGGG 2 cut(s) 480, 483
BmsI GCATC 2 cut(s) 849, 1198
BmuI ACTGGG 2 cut(s) 480, 483
BplI GAGNNNNNCTC 2 cut(s) 1041, 1073
Bpu10I CCTNAGC 1 cut(s) 1260
BpuEI CTTGAG 1 cut(s) 759
BpuMI CCSGG 2 cut(s) 465, 1256
BsaI GGTCTC 1 cut(s) 900
BsaJI CCNNGG 1 cut(s) 867
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 8 cut(s) 55, 184, 212, 520, 902, 924, 1123, 1261
Bse1I ACTGG 5 cut(s) 363, 392, 478, 486, 614
Bse21I CCTNAGG 1 cut(s) 812
Bse3DI GCAATG 2 cut(s) 1234, 1268
BseAI TCCGGA 1 cut(s) 184
BseDI CCNNGG 1 cut(s) 867
BseGI GGATG 3 cut(s) 45, 203, 388
BseLI CCNNNNNNNGG 8 cut(s) 55, 184, 212, 520, 902, 924, 1123, 1261
BseMI GCAATG 2 cut(s) 1234, 1268
BseMII CTCAG 6 cut(s) 420, 517, 803, 843, 1060, 1251
BseNI ACTGG 5 cut(s) 363, 392, 478, 486, 614
BseSI GKGCMC 1 cut(s) 1096
BseXI GCAGC 2 cut(s) 46, 1383
Bsh1285I CGRYCG 1 cut(s) 458
BshFI GGCC 3 cut(s) 501, 694, 881
BsiEI CGRYCG 1 cut(s) 458
BsiHKAI GWGCWC 2 cut(s) 71, 1096
BsiSI CCGG 3 cut(s) 185, 465, 1255
BslI CCNNNNNNNGG 8 cut(s) 55, 184, 212, 520, 902, 924, 1123, 1261
BsmAI GTCTC 1 cut(s) 900
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 3 cut(s) 501, 694, 881
Bso31I GGTCTC 1 cut(s) 900
Bsp1286I GDGCHC 2 cut(s) 71, 1096
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 5 cut(s) 187, 226, 455, 784, 955
BspANI GGCC 3 cut(s) 501, 694, 881
BspCNI CTCAG 6 cut(s) 421, 516, 804, 842, 1059, 1252
BspEI TCCGGA 1 cut(s) 184
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 3 cut(s) 182, 195, 963
BspTNI GGTCTC 1 cut(s) 900
BsrDI GCAATG 2 cut(s) 1234, 1268
BsrI ACTGG 5 cut(s) 363, 392, 478, 486, 614
BssECI CCNNGG 1 cut(s) 867
BssMI GATC 5 cut(s) 187, 226, 455, 784, 955
BssT1I CCWWGG 1 cut(s) 867
Bst4CI ACNGT 3 cut(s) 256, 582, 729
Bst6I CTCTTC 3 cut(s) 56, 236, 867
BstC8I GCNNGC 1 cut(s) 1071
BstDEI CTNAG 7 cut(s) 429, 436, 503, 812, 829, 1046, 1260
BstEII GGTNACC 2 cut(s) 552, 1052
BstF5I GGATG 3 cut(s) 45, 203, 388
BstKTI GATC 5 cut(s) 190, 229, 458, 787, 958
BstMAI GTCTC 1 cut(s) 900
BstMBI GATC 5 cut(s) 187, 226, 455, 784, 955
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 2 cut(s) 498, 1381
BstPI GGTNACC 2 cut(s) 552, 1052
BstSCI CCNGG 2 cut(s) 463, 1254
BstSLI GKGCMC 1 cut(s) 1096
BstV1I GCAGC 2 cut(s) 46, 1383
BstX2I RGATCY 2 cut(s) 187, 955
BstYI RGATCY 2 cut(s) 187, 955
Bsu36I CCTNAGG 1 cut(s) 812
BsuRI GGCC 3 cut(s) 501, 694, 881
BtsCI GGATG 3 cut(s) 45, 203, 388
BtsI GCAGTG 1 cut(s) 1332
BtsIMutI CAGTG 3 cut(s) 28, 725, 1332
Cac8I GCNNGC 1 cut(s) 1071
CciI TCATGA 1 cut(s) 277
Cfr13I GGNCC 4 cut(s) 269, 620, 692, 808
Csp6I GTAC 2 cut(s) 578, 1232
CviAII CATG 4 cut(s) 278, 934, 1106, 1251
CviQI GTAC 2 cut(s) 578, 1232
DdeI CTNAG 7 cut(s) 429, 436, 503, 812, 829, 1046, 1260
DpnI GATC 5 cut(s) 189, 228, 457, 786, 957
DpnII GATC 5 cut(s) 187, 226, 455, 784, 955
Eam1104I CTCTTC 3 cut(s) 56, 236, 867
EarI CTCTTC 3 cut(s) 56, 236, 867
Eco130I CCWWGG 1 cut(s) 867
Eco31I GGTCTC 1 cut(s) 900
Eco47I GGWCC 3 cut(s) 269, 620, 808
Eco57I CTGAAG 1 cut(s) 93
Eco81I CCTNAGG 1 cut(s) 812
Eco91I GGTNACC 2 cut(s) 552, 1052
EcoO65I GGTNACC 2 cut(s) 552, 1052
EcoRI GAATTC 1 cut(s) 1282
EcoT14I CCWWGG 1 cut(s) 867
EcoT22I ATGCAT 2 cut(s) 864, 1213
ErhI CCWWGG 1 cut(s) 867
FaeI CATG 4 cut(s) 281, 937, 1109, 1254
FaiI YATR 8 cut(s) 249, 260, 279, 920, 935, 998, 1107, 1252
FalI AAGNNNNNCTT 3 cut(s) 30, 706, 738
FatI CATG 4 cut(s) 277, 933, 1105, 1250
Fnu4HI GCNGC 2 cut(s) 60, 1397
FokI GGATG 3 cut(s) 32, 210, 375
Fsp4HI GCNGC 2 cut(s) 60, 1397
FspBI CTAG 3 cut(s) 527, 593, 1353
GluI GCNGC 2 cut(s) 60, 1397
HaeIII GGCC 3 cut(s) 501, 694, 881
HapII CCGG 3 cut(s) 185, 465, 1255
Hin1II CATG 4 cut(s) 281, 937, 1109, 1254
HincII GTYRAC 2 cut(s) 840, 975
HindII GTYRAC 2 cut(s) 840, 975
HindIII AAGCTT 2 cut(s) 715, 1067
HinfI GANTC 8 cut(s) 202, 440, 460, 985, 992, 1277, 1315, 1347
HpaI GTTAAC 1 cut(s) 975
HpaII CCGG 3 cut(s) 185, 465, 1255
HphI GGTGA 5 cut(s) 369, 581, 653, 794, 1053
Hpy166II GTNNAC 4 cut(s) 615, 840, 975, 1094
Hpy188I TCNGA 9 cut(s) 73, 175, 317, 430, 506, 789, 1003, 1049, 1141
Hpy188III TCNNGA 5 cut(s) 185, 278, 629, 854, 989
Hpy8I GTNNAC 4 cut(s) 615, 840, 975, 1094
HpyCH4III ACNGT 3 cut(s) 256, 582, 729
HpyCH4IV ACGT 1 cut(s) 1203
HpyCH4V TGCA 6 cut(s) 308, 862, 1073, 1094, 1211, 1399
HpyF10VI GCNNNNNNNGC 2 cut(s) 498, 1381
HpyF3I CTNAG 7 cut(s) 429, 436, 503, 812, 829, 1046, 1260
HpySE526I ACGT 1 cut(s) 1203
Hsp92II CATG 4 cut(s) 281, 937, 1109, 1254
Kpn2I TCCGGA 1 cut(s) 184
KspAI GTTAAC 1 cut(s) 975
Kzo9I GATC 5 cut(s) 187, 226, 455, 784, 955
LmnI GCTCC 3 cut(s) 103, 516, 1126
Lsp1109I GCAGC 2 cut(s) 46, 1383
LweI GCATC 2 cut(s) 849, 1198
MaeI CTAG 3 cut(s) 527, 593, 1353
MaeII ACGT 1 cut(s) 1203
MaeIII GTNAC 7 cut(s) 250, 552, 560, 569, 1052, 1216, 1264
MalI GATC 5 cut(s) 189, 228, 457, 786, 957
MboI GATC 5 cut(s) 187, 226, 455, 784, 955
MboII GAAGA 8 cut(s) 34, 43, 86, 223, 655, 745, 884, 1313
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 2 cut(s) 187, 955
MhlI GDGCHC 2 cut(s) 71, 1096
MlyI GAGTC 2 cut(s) 196, 1001
MmeI TCCRAC 2 cut(s) 912, 1350
Mph1103I ATGCAT 2 cut(s) 864, 1213
MroI TCCGGA 1 cut(s) 184
MroXI GAANNNNTTC 1 cut(s) 1427
MseI TTAA 8 cut(s) 542, 584, 647, 659, 974, 1007, 1131, 1403
MspI CCGG 3 cut(s) 185, 465, 1255
MspR9I CCNGG 2 cut(s) 465, 1256
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 1 cut(s) 862
MwoI GCNNNNNNNGC 2 cut(s) 498, 1381
NciI CCSGG 2 cut(s) 465, 1256
NdeII GATC 5 cut(s) 187, 226, 455, 784, 955
NlaIII CATG 4 cut(s) 281, 937, 1109, 1254
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 4 cut(s) 250, 569, 1216, 1264
NsiI ATGCAT 2 cut(s) 864, 1213
PagI TCATGA 1 cut(s) 277
PctI GAATGC 1 cut(s) 862
PdmI GAANNNNTTC 1 cut(s) 1427
PfeI GAWTC 6 cut(s) 440, 460, 985, 1277, 1315, 1347
PflFI GACNNNGTC 1 cut(s) 844
PflMI CCANNNNNTGG 3 cut(s) 520, 902, 924
PkrI GCNGC 2 cut(s) 61, 1398
Ple19I CGATCG 1 cut(s) 458
PleI GAGTC 2 cut(s) 196, 1000
PpsI GAGTC 2 cut(s) 196, 1000
PshBI ATTAAT 1 cut(s) 1403
PspEI GGTNACC 2 cut(s) 552, 1052
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 4 cut(s) 269, 620, 692, 808
PsuI RGATCY 2 cut(s) 187, 955
PsyI GACNNNGTC 1 cut(s) 844
PvuI CGATCG 1 cut(s) 458
RsaI GTAC 2 cut(s) 579, 1233
RsaNI GTAC 2 cut(s) 578, 1232
SaqAI TTAA 8 cut(s) 542, 584, 647, 659, 974, 1007, 1131, 1403
SatI GCNGC 2 cut(s) 60, 1397
Sau3AI GATC 5 cut(s) 187, 226, 455, 784, 955
Sau96I GGNCC 4 cut(s) 269, 620, 692, 808
SchI GAGTC 2 cut(s) 196, 1001
ScrFI CCNGG 2 cut(s) 465, 1256
SduI GDGCHC 2 cut(s) 71, 1096
SfaNI GCATC 2 cut(s) 849, 1198
SinI GGWCC 3 cut(s) 269, 620, 808
SmlI CTYRAG 1 cut(s) 774
SmoI CTYRAG 1 cut(s) 774
SspMI CTAG 3 cut(s) 527, 593, 1353
StyD4I CCNGG 2 cut(s) 463, 1254
StyI CCWWGG 1 cut(s) 867
TaaI ACNGT 3 cut(s) 256, 582, 729
TaiI ACGT 1 cut(s) 1206
TaqI TCGA 5 cut(s) 454, 458, 666, 1275, 1280
TfiI GAWTC 6 cut(s) 440, 460, 985, 1277, 1315, 1347
Tru1I TTAA 8 cut(s) 542, 584, 647, 659, 974, 1007, 1131, 1403
Tru9I TTAA 8 cut(s) 542, 584, 647, 659, 974, 1007, 1131, 1403
TscAI CASTG 3 cut(s) 28, 732, 1339
TseFI GTSAC 4 cut(s) 250, 569, 1216, 1264
TseI GCWGC 2 cut(s) 59, 1396
Tsp45I GTSAC 4 cut(s) 250, 569, 1216, 1264
TspDTI ATGAA 6 cut(s) 17, 34, 266, 275, 361, 1420
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 3 cut(s) 28, 732, 1339
Tth111I GACNNNGTC 1 cut(s) 844
Van91I CCANNNNNTGG 3 cut(s) 520, 902, 924
VneI GTGCAC 1 cut(s) 1092
VpaK11BI GGWCC 3 cut(s) 269, 620, 808
VspI ATTAAT 1 cut(s) 1403
XapI RAATTY 5 cut(s) 311, 480, 1133, 1282, 1321
XmnI GAANNNNTTC 1 cut(s) 1427
XspI CTAG 3 cut(s) 527, 593, 1353
Zsp2I ATGCAT 2 cut(s) 864, 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.