Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014403

Physical Location & Seq

Genomic Coordinates Reverse (-)

4681134 .. 4684912

Gene Structure 3779 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1068200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3060 bp

ATGGAAGACACAGAAAACTCCAATGGCGACCAGAAATACCACCCCAACACCAGAAGTCTGAGGGGCGGCAGCAGAGCCAATCCTCAGAAGTACCACCACCACCACCAACTAATCCGCCACACAAACCAGTTCGGTTTCTGCAACCAGAACCAGTATAATCAGAGATACTCCTCTTCCTACCCAGCTCTTCTTCCTCTCCCTCCTCTCCTCCCTCTGCAACTCGCTCTAACTCCACCTCTGCCTCCAAACCACCACTTCCGATCAAAAACCCACCTCCAGAAACATTCCTGCAGGCTCAATACTCCTCCTTTCGCGGCCTCCTCCGAAACCCAGCTGGCCCAACTCACAATTTCACCAGAAGCTTTAGAGGGGTATCAGAAGCAAACCAGTTCACCCTTTAAAGGAGAAGATGGAAGGAAGTTGCTCACAGCTGCAAGAACAGGAAAAGCAATAGTGACTGCAAGGAGGCCAGATTCTGGTGGTGTGGAGGGCACTGTAATCTCCCTCTTGGCTAACCATTTTCTTGTCCAATTCGATTTATCGCAACAAATTTTCCATTACAATGTCGACATTTCCCCTAACCCGTCGAAGGAAGTTGCTAGAATGATCAAACAGAAGCTGATCGAGGATAACTCGGCTGTGCTATCCGGTGCAATTCCAGCTTATGATGGCCGGAAGAATCTTTACAGTGCAGTCGAATTCCAAGACGATAGGCTTGAGTTCTATATAAGCCTTCCCATCCCCACAAGCAAGCCAACATTGCCATATGGAGCATTCAGTGACTTACAGGAGAAGCATCAAGAACATAAACTTTTTCGTATAAATATCAGACTTGTGTCGAAGATAGATGCGAAGGAATTGAGTAGTTACTTGAGCAAGGAGGGCAATGAATGGAAGCCGATTCCTCAGGATTATCTCCATGCCTTGGATGTCGTCTTGAGGGAGGCGCCGTTGGAGAAGTGTGTACCTGTGGGAAGATCACTCTATTCGAGTTCAATGGGAGGAAGTAAAGAAATTGGAGGAGGGGCTGTAGGACTGAGAGGGTTCTTCCAGAGCCTTCGACCAACGCAACAAGGACTAGCTCTCAACGTTGATTTCTCCGTGACTGCATTCCACGAAAGCGTTGGAGTGATCCCATACTTGCAGAAGCGCCTCGAGTTTCTTCAAGACCTTCCTCAAAGGAAGACAAGGGGCTTAACTGATAAAGAAAGGAAAGAAGTGGAGAAGGCATTGAAGAACATCAGGGTCTTTGTTTGCCACAGAGAAACTGTCCAGAGATACCGCGTTTGTGGCTTGACCGAAGAAGCCACAGAAAATCTTTGGTTTGCAGACAGGGATGGGAAGAATTTGAGGCTGTTGACTTACTTCAAGGATCACTATAATTATGATATACAATTCAGGAATTTGCCTTGTTTGCAGATTAGTAGGAGTAAACCGTGCTATCTTCCGATGGAGCTATGCATGATCTGTGAAGGCCAGAAGTTTCTCGGGAAACTGTCCGATGATCAAACTGCAAGGATACTTAAGATGGGCTGCCAACGCCCCAAAGAACGAAAAGCCATTATAGATGGAGTCATGAGAGGTCCTGTTGGGCCAACAAGTGGCATTCAGGAAAGAGAATTCAAGCTCCATGTTTCGAGAGACATGACTCGATTAAAAGGAAGAGTTCTTCAACCTCCAAAGCTAAAGCTTGGTGATGGTGGTCACGTAAGAGACCTAGTTCCCTCTCGTCTTGATCGGCAATGGAATCTTATAAACAGCCATGTGTTTGAAGGTACTCGAATCGAGAGGTGGGCGTTGATTGGTTTCGGTGGCACCCCTGATCAGAAGAATAGCATCCCAAAATTCATACGCCAGCTATCTCAAAGATGTGAACAATTGGGAATCTTCCTCAACAAGAACACGATTGTTAGCCCCCAATTCGAACCATTCCAAGTGCTTAACAATGTGTCGCTGTTAGAATCCAAGCTCAAAAGAATCCAAAGAGCTGCATCAAACAACCTCCAGCTGCTTATGTGTGTGATGGAAAGAAAGCACAAAGGCTACGCAGATCTCAAGCGAATTGCGGATACAAGCGTTGGGATTTTAAGCCAATGCTGCTTGTACTCAAACCTCGGGAAGATGGGTTCACAGTTTTTGGCAAATCTTGCTCTCAAGATCAATGCAAAAGTTGGAGGATGCACGGTTTCTTTGTACAATTCATTACCAACACAAATCCCGCGGCTGCTTCAGGCTGGTGAACCGGTGATCTTTATGGGTGCGGATGTGACTCATCCGCACCCACTCGATGATTTTAGTCCTTCTGTTGCTGCTGTGGTTGGTAGCATGAACTGGCCGGCGGCAAATAAGTATGTGTCAAGAATGAGGTCTCAGACGCATCGACAAGAAATCATCCAGGATCTCGACGCAATGGTGGAAGAATTATTGAATGAGTTTCGCCAGGAAGTTGGCAAACTTCCCAAGAGAATCATTTTCTTCAGAGACGGGGTAAGCGAAACGCAATTCTACAAAGTGCTTCAAGAGGAGTTGCAAGCCATTAAAGGGGCATGTTCAAATTTTCCCGGTTATGCACCTCCCATAACATTTGCAGTGGTTCAAAAGAGGCATCACACAAGGCTGTTCCCTTTCAAAATTGATCTGTCTTCGAAGCAGAACCAGTTACTCGACGAAAACATTCCCCCTGGAACCGTTGTGGATACCGTGATCACTCACCCGAAAGAATTCGACTTCTATCTTTGCAGCCATTGGGGTGTTAAGGGGACAAGCAGGCCAACTCATTACCACATATTGTGGGATGAAAACCTGTTCACTTCAGATGAACTGCAGAAGCTGGTCAACATTCTGTGCTACACTTACGCAAGGTGCACGAAGCCGGTTTCTCTGGTGCCCCCGGCTTACTACGCTCACTTGGCGGCATATCGAGGCAGGCTTTACCTTGAGAGGTCAGAATCCACAACTTATACCAGAAGTGGTTCGACACTCACCAGAGCTGGCCCTCCGAAGGAAATGGCTTTACCAAAACTTAGTGAGAATGTTAAGAAACTCATGTTTTACTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009888 GO:0009892 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0019827 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035821 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043207 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0098542 GO:0098586 GO:0098727 GO:1901360 GO:1901698 GO:1901699

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

1020

Amino Acids

115.59

Weight (kDa)

9.36

Isoelectric Point (pI)

45.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	166 - 313	9.9e-29	N-terminal domain of argonaute
ArgoL1	PF08699	323 - 372	2.5e-16	Argonaute linker 1 domain
PAZ	PF02170	385 - 507	1.8e-28	PAZ domain
ArgoL2	PF16488	517 - 563	6.2e-07	Argonaute linker 2 domain
Piwi	PF02171	671 - 977	1.1e-93	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69440
fragaria_vesca	FvH4_4g30730
malus_domestica	MD13G1068200.v1.1 MD16G1067000.v1.1
prunus_persica	Prupe.1G279900_v2.0.a1
pyrus_communis	pycom13g06000 pycom16g05840
rosa_chinensis	RchiOBHm_Chr4g0439121
rosa_laevigata	RLG00000006296
rosa_roxburghii	Rroxscaffold_5G00380110
rosa_rugosa	Rorug04G0315900
rosa_samantha	Rh4AG366700 Rh4BG378200 Rh4CG392300 Rh4DG372800
rosa_wichuraiana	Rw0G007490 Rw0G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1754
AccB1I	GGYRCC	3 cut(s)	946, 1814, 2884
AccB7I	CCANNNNNTGG	3 cut(s)	476, 925, 1601
AccI	GTMKAC	1 cut(s)	567
AccII	CGCG	3 cut(s)	314, 1284, 2221
AclI	AACGTT	1 cut(s)	1089
AclWI	GGATC	3 cut(s)	1126, 1380, 2406
AcoI	YGGCCR	2 cut(s)	670, 2333
AcsI	RAATTY	8 cut(s)	549, 698, 1345, 1402, 1619, 1844, 2554, 2720
AcuI	CTGAAG	3 cut(s)	2213, 2461, 2796
AcyI	GRCGYC	1 cut(s)	947
AfaI	GTAC	5 cut(s)	92, 966, 1777, 2105, 2195
AfiI	CCNNNNNNNGG	7 cut(s)	401, 476, 589, 925, 1601, 2541, 3001
AflII	CTTAAG	1 cut(s)	1523
AgeI	ACCGGT	1 cut(s)	2242
AjnI	CCWGG	3 cut(s)	2394, 2439, 2680
AloI	GAACNNNNNNTCC	2 cut(s)	2110, 2142
Alw21I	GWGCWC	1 cut(s)	2867
Alw26I	GTCTC	4 cut(s)	1635, 1707, 2373, 2475
Alw44I	GTGCAC	1 cut(s)	2863
AlwI	GGATC	3 cut(s)	1126, 1380, 2406
AlwNI	CAGNNNCTG	3 cut(s)	476, 619, 2830
Ama87I	CYCGRG	3 cut(s)	1154, 1487, 2114
AoxI	GGCC	9 cut(s)	315, 336, 467, 670, 1474, 1592, 2333, 2768, 2992
ApaLI	GTGCAC	1 cut(s)	2863
ApeKI	GCWGC	9 cut(s)	69, 431, 1533, 1988, 2008, 2097, 2224, 2309, 2739
ApoI	RAATTY	8 cut(s)	549, 698, 1345, 1402, 1619, 1844, 2554, 2720
AsiGI	ACCGGT	1 cut(s)	2242
Asp700I	GAANNNNTTC	3 cut(s)	2673, 2720, 2971
AspLEI	GCGC	2 cut(s)	949, 1152
AspS9I	GGNCC	4 cut(s)	337, 1583, 1592, 2993
AsuC2I	CCSGG	2 cut(s)	2562, 2891
AsuHPI	GGTGA	7 cut(s)	345, 384, 1706, 2249, 2257, 2702, 2974
AsuII	TTCGAA	2 cut(s)	1923, 2645
AvaI	CYCGRG	3 cut(s)	1154, 1487, 2114
AvaII	GGWCC	1 cut(s)	1583
AxyI	CCTNAGG	1 cut(s)	906
BaeGI	GKGCMC	3 cut(s)	494, 2867, 2889
BaeI	ACNNNNGTAYC	2 cut(s)	2061, 2094
BanI	GGYRCC	3 cut(s)	946, 1814, 2884
BarI	GAAGNNNNNNTAC	2 cut(s)	668, 700
BbsI	GAAGAC	3 cut(s)	12, 1190, 2634
Bbv12I	GWGCWC	1 cut(s)	2867
BbvI	GCAGC	9 cut(s)	81, 418, 1520, 1975, 1995, 2084, 2211, 2296, 2751
BceAI	ACGGC	1 cut(s)	934
BciT130I	CCWGG	3 cut(s)	2396, 2441, 2682
BciVI	GTATCC	3 cut(s)	1512, 2062, 2689
BclI	TGATCA	4 cut(s)	606, 1504, 1822, 2703
BcnI	CCSGG	2 cut(s)	2562, 2891
BcoDI	GTCTC	4 cut(s)	1635, 1707, 2373, 2475
BfaI	CTAG	3 cut(s)	600, 1079, 1718
BfmI	CTRYAG	3 cut(s)	289, 1029, 2822
BfoI	RGCGCY	2 cut(s)	950, 1153
BfrI	CTTAAG	1 cut(s)	1523
BfuI	GTATCC	3 cut(s)	1512, 2062, 2689
BglII	AGATCT	1 cut(s)	2050
Bme1390I	CCNGG	5 cut(s)	2396, 2441, 2562, 2682, 2891
Bme18I	GGWCC	1 cut(s)	1583
BmeT110I	CYCGRG	3 cut(s)	1154, 1487, 2114
BmgT120I	GGNCC	4 cut(s)	337, 1583, 1592, 2993
BmiI	GGNNCC	4 cut(s)	948, 1816, 2686, 2886
BmrFI	CCNGG	5 cut(s)	2396, 2441, 2562, 2682, 2891
BmsI	GCATC	7 cut(s)	805, 838, 1845, 2000, 2168, 2386, 2614
BoxI	GACNNNNGTC	1 cut(s)	835
BpiI	GAAGAC	3 cut(s)	12, 1190, 2634
BplI	GAGNNNNNCTC	2 cut(s)	617, 649
BpmI	CTGGAG	2 cut(s)	260, 1988
Bpu14I	TTCGAA	2 cut(s)	1923, 2645
BpuEI	CTTGAG	6 cut(s)	737, 892, 958, 2039, 2138, 2957
BpuMI	CCSGG	2 cut(s)	2562, 2891
BsaAI	YACGTR	1 cut(s)	1708
BsaHI	GRCGYC	1 cut(s)	947
BsaI	GGTCTC	2 cut(s)	1707, 2373
BsaJI	CCNNGG	5 cut(s)	924, 2113, 2219, 2680, 2889
BsaWI	WCCGGW	2 cut(s)	647, 2242
BsaXI	ACNNNNNCTCC	4 cut(s)	935, 965, 1014, 1044
Bsc4I	CCNNNNNNNGG	7 cut(s)	401, 476, 589, 925, 1601, 2541, 3001
Bse118I	RCCGGY	3 cut(s)	2242, 2335, 2872
Bse1I	ACTGG	5 cut(s)	127, 151, 387, 2336, 2656
Bse21I	CCTNAGG	1 cut(s)	906
Bse3DI	GCAATG	4 cut(s)	758, 892, 1748, 2415
BseBI	CCWGG	3 cut(s)	2396, 2441, 2682
BseDI	CCNNGG	5 cut(s)	924, 2113, 2219, 2680, 2889
BseGI	GGATG	9 cut(s)	738, 934, 1342, 1836, 2183, 2269, 2272, 2391, 2800
BseLI	CCNNNNNNNGG	7 cut(s)	401, 476, 589, 925, 1601, 2541, 3001
BseMI	GCAATG	4 cut(s)	758, 892, 1748, 2415
BseMII	CTCAG	5 cut(s)	50, 98, 920, 1028, 2384
BseNI	ACTGG	5 cut(s)	127, 151, 387, 2336, 2656
BseRI	GAGGAG	7 cut(s)	160, 192, 197, 294, 310, 1035, 2537
BseSI	GKGCMC	3 cut(s)	494, 2867, 2889
BseXI	GCAGC	9 cut(s)	81, 418, 1520, 1975, 1995, 2084, 2211, 2296, 2751
BseYI	CCCAGC	2 cut(s)	181, 330
BsgI	GTGCAG	1 cut(s)	711
Bsh1236I	CGCG	3 cut(s)	314, 1284, 2221
BshFI	GGCC	9 cut(s)	317, 338, 469, 672, 1476, 1594, 2335, 2770, 2994
BshNI	GGYRCC	3 cut(s)	946, 1814, 2884
BshTI	ACCGGT	1 cut(s)	2242
BsiHKAI	GWGCWC	1 cut(s)	2867
BsiHKCI	CYCGRG	3 cut(s)	1154, 1487, 2114
BsiSI	CCGG	7 cut(s)	648, 673, 2243, 2336, 2562, 2873, 2891
BslFI	GGGAC	1 cut(s)	2773
BslI	CCNNNNNNNGG	7 cut(s)	401, 476, 589, 925, 1601, 2541, 3001
BsmAI	GTCTC	4 cut(s)	1635, 1707, 2373, 2475
BsmBI	CGTCTC	1 cut(s)	2475
BsmFI	GGGAC	1 cut(s)	2773
BsmI	GAATGC	3 cut(s)	773, 1109, 1605
BsnI	GGCC	9 cut(s)	317, 338, 469, 672, 1476, 1594, 2335, 2770, 2994
Bso31I	GGTCTC	2 cut(s)	1707, 2373
BsoBI	CYCGRG	3 cut(s)	1154, 1487, 2114
Bsp119I	TTCGAA	2 cut(s)	1923, 2645
Bsp1286I	GDGCHC	3 cut(s)	494, 2867, 2889
Bsp1407I	TGTACA	1 cut(s)	2193
BspANI	GGCC	9 cut(s)	317, 338, 469, 672, 1476, 1594, 2335, 2770, 2994
BspCNI	CTCAG	5 cut(s)	51, 97, 919, 1029, 2383
BspFNI	CGCG	3 cut(s)	314, 1284, 2221
BspHI	TCATGA	1 cut(s)	1575
BspLI	GGNNCC	4 cut(s)	948, 1816, 2686, 2886
BspMAI	CTGCAG	2 cut(s)	293, 2826
BspPI	GGATC	3 cut(s)	1126, 1380, 2406
BspQI	GCTCTTC	1 cut(s)	192
BspT104I	TTCGAA	2 cut(s)	1923, 2645
BspT107I	GGYRCC	3 cut(s)	946, 1814, 2884
BspTI	CTTAAG	1 cut(s)	1523
BspTNI	GGTCTC	2 cut(s)	1707, 2373
BsrDI	GCAATG	4 cut(s)	758, 892, 1748, 2415
BsrFI	RCCGGY	3 cut(s)	2242, 2335, 2872
BsrGI	TGTACA	1 cut(s)	2193
BsrI	ACTGG	5 cut(s)	127, 151, 387, 2336, 2656
BssAI	RCCGGY	3 cut(s)	2242, 2335, 2872
BssECI	CCNNGG	5 cut(s)	924, 2113, 2219, 2680, 2889
BssNI	GRCGYC	1 cut(s)	947
BssT1I	CCWWGG	1 cut(s)	924
Bst2UI	CCWGG	3 cut(s)	2396, 2441, 2682
Bst4CI	ACNGT	9 cut(s)	496, 689, 1270, 1437, 1497, 2133, 2185, 2689, 2701
Bst6I	CTCTTC	3 cut(s)	178, 192, 1657
BstACI	GRCGYC	1 cut(s)	947
BstAFI	CTTAAG	1 cut(s)	1523
BstAPI	GCANNNNNTGC	1 cut(s)	2147
BstAUI	TGTACA	1 cut(s)	2193
BstBAI	YACGTR	1 cut(s)	1708
BstBI	TTCGAA	2 cut(s)	1923, 2645
BstC8I	GCNNGC	9 cut(s)	293, 336, 752, 1856, 2337, 2532, 2768, 2927, 2992
BstDEI	CTNAG	6 cut(s)	59, 84, 906, 1037, 2370, 3023
BstDSI	CCRYGG	1 cut(s)	2219
BstF5I	GGATG	9 cut(s)	738, 934, 1342, 1836, 2183, 2269, 2272, 2391, 2800
BstFNI	CGCG	3 cut(s)	314, 1284, 2221
BstH2I	RGCGCY	2 cut(s)	950, 1153
BstHHI	GCGC	2 cut(s)	949, 1152
BstMAI	GTCTC	4 cut(s)	1635, 1707, 2373, 2475
BstNI	CCWGG	3 cut(s)	2396, 2441, 2682
BstNSI	RCATGY	1 cut(s)	2550
BstPAI	GACNNNNGTC	1 cut(s)	835
BstSCI	CCNGG	5 cut(s)	2394, 2439, 2560, 2680, 2889
BstSFI	CTRYAG	3 cut(s)	289, 1029, 2822
BstSLI	GKGCMC	3 cut(s)	494, 2867, 2889
BstUI	CGCG	3 cut(s)	314, 1284, 2221
BstV1I	GCAGC	9 cut(s)	81, 418, 1520, 1975, 1995, 2084, 2211, 2296, 2751
BstV2I	GAAGAC	3 cut(s)	12, 1190, 2634
BstX2I	RGATCY	2 cut(s)	2050, 2398
BstXI	CCANNNNNNTGG	1 cut(s)	2447
BstYI	RGATCY	2 cut(s)	2050, 2398
Bsu36I	CCTNAGG	1 cut(s)	906
BsuI	GTATCC	3 cut(s)	1512, 2062, 2689
BsuRI	GGCC	9 cut(s)	317, 338, 469, 672, 1476, 1594, 2335, 2770, 2994
BtgI	CCRYGG	1 cut(s)	2219
BtsCI	GGATG	9 cut(s)	738, 934, 1342, 1836, 2183, 2269, 2272, 2391, 2800
BtsI	GCAGTG	1 cut(s)	2595
BtsIMutI	CAGTG	4 cut(s)	492, 694, 784, 2595
Cac8I	GCNNGC	9 cut(s)	293, 336, 752, 1856, 2337, 2532, 2768, 2927, 2992
CaiI	CAGNNNCTG	3 cut(s)	476, 619, 2830
CciI	TCATGA	1 cut(s)	1575
CfoI	GCGC	2 cut(s)	949, 1152
Cfr10I	RCCGGY	3 cut(s)	2242, 2335, 2872
Cfr13I	GGNCC	4 cut(s)	337, 1583, 1592, 2993
Cfr42I	CCGCGG	1 cut(s)	2222
CseI	GACGC	2 cut(s)	2383, 2414
Csp6I	GTAC	5 cut(s)	91, 965, 1776, 2104, 2194
CspAI	ACCGGT	1 cut(s)	2242
CviAII	CATG	9 cut(s)	920, 1462, 1576, 1631, 1645, 1763, 2326, 2547, 3046
CviQI	GTAC	5 cut(s)	91, 965, 1776, 2104, 2194
DdeI	CTNAG	6 cut(s)	59, 84, 906, 1037, 2370, 3023
DinI	GGCGCC	1 cut(s)	948
DraI	TTTAAA	1 cut(s)	400
EaeI	YGGCCR	2 cut(s)	670, 2333
Eam1104I	CTCTTC	3 cut(s)	178, 192, 1657
EarI	CTCTTC	3 cut(s)	178, 192, 1657
EciI	GGCGGA	1 cut(s)	104
Eco130I	CCWWGG	1 cut(s)	924
Eco31I	GGTCTC	2 cut(s)	1707, 2373
Eco47I	GGWCC	1 cut(s)	1583
Eco57I	CTGAAG	3 cut(s)	2213, 2461, 2796
Eco81I	CCTNAGG	1 cut(s)	906
Eco88I	CYCGRG	3 cut(s)	1154, 1487, 2114
EcoO109I	RGGNCCY	1 cut(s)	1583
EcoRI	GAATTC	3 cut(s)	698, 1619, 2720
EcoRII	CCWGG	3 cut(s)	2394, 2439, 2680
EcoT14I	CCWWGG	1 cut(s)	924
EcoT22I	ATGCAT	1 cut(s)	1463
EgeI	GGCGCC	1 cut(s)	948
EheI	GGCGCC	1 cut(s)	948
ErhI	CCWWGG	1 cut(s)	924
Esp3I	CGTCTC	1 cut(s)	2475
FaeI	CATG	9 cut(s)	923, 1465, 1579, 1634, 1648, 1766, 2329, 2550, 3049
FaqI	GGGAC	1 cut(s)	2773
FatI	CATG	9 cut(s)	919, 1461, 1575, 1630, 1644, 1762, 2325, 2546, 3045
FauI	CCCGC	1 cut(s)	2226
FauNDI	CATATG	1 cut(s)	766
FbaI	TGATCA	4 cut(s)	606, 1504, 1822, 2703
FblI	GTMKAC	1 cut(s)	567
FokI	GGATG	9 cut(s)	725, 941, 1349, 1823, 2190, 2259, 2276, 2378, 2807
FspBI	CTAG	3 cut(s)	600, 1079, 1718
GlaI	GCGC	2 cut(s)	948, 1151
GsaI	CCCAGC	2 cut(s)	185, 334
GsuI	CTGGAG	2 cut(s)	260, 1988
HaeII	RGCGCY	2 cut(s)	950, 1153
HaeIII	GGCC	9 cut(s)	317, 338, 469, 672, 1476, 1594, 2335, 2770, 2994
HapII	CCGG	7 cut(s)	648, 673, 2243, 2336, 2562, 2873, 2891
HgaI	GACGC	2 cut(s)	2383, 2414
HhaI	GCGC	2 cut(s)	949, 1152
Hin1I	GRCGYC	1 cut(s)	947
Hin1II	CATG	9 cut(s)	923, 1465, 1579, 1634, 1648, 1766, 2329, 2550, 3049
Hin6I	GCGC	2 cut(s)	947, 1150
HinP1I	GCGC	2 cut(s)	947, 1150
HincII	GTYRAC	3 cut(s)	568, 1359, 2836
HindII	GTYRAC	3 cut(s)	568, 1359, 2836
HindIII	AAGCTT	2 cut(s)	360, 1688
HpaII	CCGG	7 cut(s)	648, 673, 2243, 2336, 2562, 2873, 2891
HphI	GGTGA	7 cut(s)	345, 384, 1706, 2249, 2257, 2702, 2974
Hpy99I	CGWCG	3 cut(s)	589, 2408, 2669
HpyCH4III	ACNGT	9 cut(s)	496, 689, 1270, 1437, 1497, 2133, 2185, 2689, 2701
HpyCH4IV	ACGT	2 cut(s)	1089, 1707
HpyF3I	CTNAG	6 cut(s)	59, 84, 906, 1037, 2370, 3023
HpySE526I	ACGT	2 cut(s)	1089, 1707
Hsp92I	GRCGYC	1 cut(s)	947
Hsp92II	CATG	9 cut(s)	923, 1465, 1579, 1634, 1648, 1766, 2329, 2550, 3049
HspAI	GCGC	2 cut(s)	947, 1150
KasI	GGCGCC	1 cut(s)	946
KroI	GCCGGC	1 cut(s)	2335
KroNI	GCCGGC	1 cut(s)	2337
Ksp22I	TGATCA	4 cut(s)	606, 1504, 1822, 2703
KspI	CCGCGG	1 cut(s)	2222
LguI	GCTCTTC	1 cut(s)	192
LmnI	GCTCC	3 cut(s)	770, 1453, 1632
Lsp1109I	GCAGC	9 cut(s)	81, 418, 1520, 1975, 1995, 2084, 2211, 2296, 2751
LweI	GCATC	7 cut(s)	805, 838, 1845, 2000, 2168, 2386, 2614
MaeI	CTAG	3 cut(s)	600, 1079, 1718
MaeII	ACGT	2 cut(s)	1089, 1707
MaeIII	GTNAC	7 cut(s)	454, 779, 866, 1102, 1703, 2266, 2658
MfeI	CAATTG	1 cut(s)	1877
MflI	RGATCY	2 cut(s)	2050, 2398
MhlI	GDGCHC	3 cut(s)	494, 2867, 2889
Mly113I	GGCGCC	1 cut(s)	947
MlyI	GAGTC	3 cut(s)	1581, 1642, 2263
MmeI	TCCRAC	3 cut(s)	933, 1105, 2152
Mph1103I	ATGCAT	1 cut(s)	1463
MroNI	GCCGGC	1 cut(s)	2335
MroXI	GAANNNNTTC	3 cut(s)	2673, 2720, 2971
MseI	TTAA	9 cut(s)	399, 1196, 1524, 1655, 1941, 2087, 2538, 2754, 3036
MslI	CAYNNNNRTG	2 cut(s)	561, 2748
MspA1I	CMGCKG	4 cut(s)	334, 431, 2008, 2221
MspCI	CTTAAG	1 cut(s)	1523
MspI	CCGG	7 cut(s)	648, 673, 2243, 2336, 2562, 2873, 2891
MspR9I	CCNGG	5 cut(s)	2396, 2441, 2562, 2682, 2891
MunI	CAATTG	1 cut(s)	1877
Mva1269I	GAATGC	3 cut(s)	773, 1109, 1605
MvaI	CCWGG	3 cut(s)	2396, 2441, 2682
MvnI	CGCG	3 cut(s)	314, 1284, 2221
NaeI	GCCGGC	1 cut(s)	2337
NarI	GGCGCC	1 cut(s)	947
NciI	CCSGG	2 cut(s)	2562, 2891
NdeI	CATATG	1 cut(s)	766
NgoMIV	GCCGGC	1 cut(s)	2335
NlaIII	CATG	9 cut(s)	923, 1465, 1579, 1634, 1648, 1766, 2329, 2550, 3049
NlaIV	GGNNCC	4 cut(s)	948, 1816, 2686, 2886
NmeAIII	GCCGAG	1 cut(s)	614
NmuCI	GTSAC	5 cut(s)	454, 779, 1102, 1703, 2266
NsiI	ATGCAT	1 cut(s)	1463
NspI	RCATGY	1 cut(s)	2550
NspV	TTCGAA	2 cut(s)	1923, 2645
PaeR7I	CTCGAG	1 cut(s)	1154
PagI	TCATGA	1 cut(s)	1575
PciSI	GCTCTTC	1 cut(s)	192
PcsI	WCGNNNNNNNCGW	1 cut(s)	2490
PctI	GAATGC	3 cut(s)	773, 1109, 1605
PdiI	GCCGGC	1 cut(s)	2337
PdmI	GAANNNNTTC	3 cut(s)	2673, 2720, 2971
PflMI	CCANNNNNTGG	3 cut(s)	476, 925, 1601
PfoI	TCCNGGA	1 cut(s)	2394
PinAI	ACCGGT	1 cut(s)	2242
PleI	GAGTC	3 cut(s)	1580, 1642, 2263
PluTI	GGCGCC	1 cut(s)	950
PpsI	GAGTC	3 cut(s)	1580, 1642, 2263
Ppu21I	YACGTR	1 cut(s)	1708
PpuMI	RGGWCCY	1 cut(s)	1583
PshAI	GACNNNNGTC	1 cut(s)	835
PsiI	TTATAA	1 cut(s)	1754
Psp1406I	AACGTT	1 cut(s)	1089
Psp5II	RGGWCCY	1 cut(s)	1583
Psp6I	CCWGG	3 cut(s)	2394, 2439, 2680
PspFI	CCCAGC	2 cut(s)	181, 330
PspGI	CCWGG	3 cut(s)	2394, 2439, 2680
PspN4I	GGNNCC	4 cut(s)	948, 1816, 2686, 2886
PspPI	GGNCC	4 cut(s)	337, 1583, 1592, 2993
PspPPI	RGGWCCY	1 cut(s)	1583
PspXI	VCTCGAGB	1 cut(s)	1154
PstI	CTGCAG	2 cut(s)	293, 2826
PstNI	CAGNNNCTG	3 cut(s)	476, 619, 2830
PsuI	RGATCY	2 cut(s)	2050, 2398
PvuII	CAGCTG	3 cut(s)	334, 431, 2008
RsaI	GTAC	5 cut(s)	92, 966, 1777, 2105, 2195
RsaNI	GTAC	5 cut(s)	91, 965, 1776, 2104, 2194
RseI	CAYNNNNRTG	2 cut(s)	561, 2748
SacII	CCGCGG	1 cut(s)	2222
SalI	GTCGAC	1 cut(s)	566
SapI	GCTCTTC	1 cut(s)	192
SaqAI	TTAA	9 cut(s)	399, 1196, 1524, 1655, 1941, 2087, 2538, 2754, 3036
Sau96I	GGNCC	4 cut(s)	337, 1583, 1592, 2993
SbfI	CCTGCAGG	1 cut(s)	293
SchI	GAGTC	3 cut(s)	1581, 1642, 2263
ScrFI	CCNGG	5 cut(s)	2396, 2441, 2562, 2682, 2891
SdaI	CCTGCAGG	1 cut(s)	293
SduI	GDGCHC	3 cut(s)	494, 2867, 2889
SfaNI	GCATC	7 cut(s)	805, 838, 1845, 2000, 2168, 2386, 2614
SfcI	CTRYAG	3 cut(s)	289, 1029, 2822
SfoI	GGCGCC	1 cut(s)	948
Sfr274I	CTCGAG	1 cut(s)	1154
Sfr303I	CCGCGG	1 cut(s)	2222
SfuI	TTCGAA	2 cut(s)	1923, 2645
SgrBI	CCGCGG	1 cut(s)	2222
SinI	GGWCC	1 cut(s)	1583
SlaI	CTCGAG	1 cut(s)	1154
SmiMI	CAYNNNNRTG	2 cut(s)	561, 2748
SmlI	CTYRAG	8 cut(s)	716, 871, 937, 1154, 1523, 2054, 2153, 2936
SmoI	CTYRAG	8 cut(s)	716, 871, 937, 1154, 1523, 2054, 2153, 2936
Sse8387I	CCTGCAGG	1 cut(s)	293
SspDI	GGCGCC	1 cut(s)	946
SspMI	CTAG	3 cut(s)	600, 1079, 1718
StyD4I	CCNGG	5 cut(s)	2394, 2439, 2560, 2680, 2889
StyI	CCWWGG	1 cut(s)	924
TaaI	ACNGT	9 cut(s)	496, 689, 1270, 1437, 1497, 2133, 2185, 2689, 2701
TaiI	ACGT	2 cut(s)	1092, 1710
TaqII	GACCGA	1 cut(s)	1313
TatI	WGTACW	2 cut(s)	2103, 2193
TauI	GCSGC	5 cut(s)	69, 317, 2224, 2342, 2915
Tru1I	TTAA	9 cut(s)	399, 1196, 1524, 1655, 1941, 2087, 2538, 2754, 3036
Tru9I	TTAA	9 cut(s)	399, 1196, 1524, 1655, 1941, 2087, 2538, 2754, 3036
TscAI	CASTG	4 cut(s)	499, 694, 784, 2595
TseFI	GTSAC	5 cut(s)	454, 779, 1102, 1703, 2266
TseI	GCWGC	9 cut(s)	69, 431, 1533, 1988, 2008, 2097, 2224, 2309, 2739
Tsp45I	GTSAC	5 cut(s)	454, 779, 1102, 1703, 2266
TspDTI	ATGAA	6 cut(s)	903, 1837, 2190, 2342, 2811, 2832
TspGWI	ACGGA	1 cut(s)	1090
TspRI	CASTG	4 cut(s)	499, 694, 784, 2595
Van91I	CCANNNNNTGG	3 cut(s)	476, 925, 1601
Vha464I	CTTAAG	1 cut(s)	1523
VneI	GTGCAC	1 cut(s)	2863
VpaK11BI	GGWCC	1 cut(s)	1583
XapI	RAATTY	8 cut(s)	549, 698, 1345, 1402, 1619, 1844, 2554, 2720
XceI	RCATGY	1 cut(s)	2550
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1121
XhoI	CTCGAG	1 cut(s)	1154
XmiI	GTMKAC	1 cut(s)	567
XmnI	GAANNNNTTC	3 cut(s)	2673, 2720, 2971
XspI	CTAG	3 cut(s)	600, 1079, 1718
Zsp2I	ATGCAT	1 cut(s)	1463

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.