Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014403

Physical Location & Seq

Genomic Coordinates Reverse (-)

4718745 .. 4722564

Gene Structure 3820 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1067000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3069 bp

ATGGCAGACACAGAAAACTCCAATGGCAACCAGAAATGCCACCCCAAGACCAGAACCCTGAGGGGCGGCACCAGAGCCAATCCTCACAACCACCACCACCACCACCACCACCAACAACTAATCCACCACGCAAACCAGTTCGGTTTCTGCAACCAGAACCAGTATTATCAGAGATACTCCTCCTCCTACCCAGCTCTTCTTCCTCTCCCTCCTCTCCTCCCTCTGCAACTCGCTCTAACTCCACCGCTGCCTCCAAACCACCACTTCCGATCAAAAACCCACCTCCAGAAACCTTCATGCAGGCTCAATACTCCTCCTTTTGCCGCCTCCTCCAAAACCCAGGTGCCCCAACTCACAATTTCACCAAAAGCTACAGAGGGGTATCAGAAGCAAACCAGTTCACCATTTATAGGAGAAGATGGAAGGAAGTTGCTCACAGCTGCAAGAACAGGAAAAGCAATAGTGACTGCAAGGAGGCCAGATTCTGGTGGTGTGGAAGGGACTGTAATCTCCCTCTTGGCTAACCATTTTCTTGTCCAATTCGATTCATCGCAGCGAATATTCCATTACCATGTTGACATTTCCCCTAACCCCTCGAAGGAAGTTGCTAGAATGATCAAACAGAAGCTGATGGAGGATAACTCGGCTGTGCTATCCGGTGCAATTCCGGCTTATGATGGCCGGAAGAATCTTTACAGCGCCCTTGAATTCCGAGACGATAGGCTTGAATTCTATGTAAGCCTCCTCATCCCCACAAGTAAGCCAACATTGCCATATGGGGCATTCAATGACTTACAAGAGAAGCTTCAAGAACATAAACTTTTTCGGGTCAATATCAAACTCGTGTCAAAGCTAGATGCGAAGGAATTGAGTAGTTACTTGAACAATGAGGGTGATGAATGGAAGCCGCTTCCTCAGGATTATCTCCACGCCTTGGATGTCGTCTTGAGGGAGGCACCATTGGAGAAGTGTGTGCCTGTGGGAAGATCACTCTACTCGAGCTCAATGGGTGGAAGTAAAGAAATTGGAGGAGGGGCCGTAGGACTGAGAGGGTTCTTTCAGAGCCTTCGACTAACTCAACAAGGACTAGCTCTCAATGTTGATTTCTCCGTGACTGCATTCCACGAAAGCGTTGGAGTGATCCCATACTTACAGAAGCGCCTCGAGTTTCTTCAAGACCTTCCTCAAAGGAAGACAAGGGGTTTAACTGAGAAAGAAAGGAAAGAAGTGGAGAAGGCATTGAAGAATATCAGGGTCTTTGTTTGCCACCGAGAAACTGTTCAGAGATACCGCGTTTTTGGCTTAACCGAGGAAGCCACTGAAGATCTTTGGTTTGCAGACAGGGATGGGAAGAATTTGAGGCTGTTGACTTACTTTAAGGATCACTATAACTATGATATACAATTCAGGAATTTGCCTTGTTTGCAGATTAGTAGGAGTAAACCATGCTATCTTCCAATGGAGCTATGTATGATCTGTGAAGGCCAGAAGTTCCTCGGGAAGCTGTCTGATGATCAGACTGCAAGGATACTTAAGATGGGCTGCCAACGACCGAAAGAACGAAAAGCCATTATAGATGGAGTTATGAGAGGTCCTGTTGGGCCAACAAGTGGCATTCAGGAAAGAGAATTCAAGCTCCATGTTTCGAGAGACATGACGCGATTAAAAGGAAGAGTTCTTCAACCTCCAAAGCTAAAGCTTGGCGATGGTGGTCATGTAAGGGACCTAATTCCCTCTCGTCTTGACCGGCAGTGGAATCTTATGGACAGCCATGTGTTTGAAGGTACTAGAATCGAGAGGTGGGCATTGATTAGTTTTGGTGGCACCCCTGATCAGAAGAATAGCATCCCGAAATTCATACGCCAGCTATCTCAAAGATGTGAACAACTGGGAATCTTCCTTAACAATAACACGATTGTTAGCCCCCAATTCCAACCATCCCAAGTGCTTAACAATGTGTCGCTGTTAGAATCTAAGCTCAAAAGAATCCAAAGAGCTGCATCAAACAATCTCCAGCTGCTTATATGTGTAATGGAAAGAAAGCACAAAGGCTACGCAGATCTCAAGCGAATTGCGGACACAAGCGTTGGGGTTTTAAGCCAATGCTGCTTGTACTCAAACCTCGGGAAGTTGGGTTCGCAGTTTTTGGCGAACCTAGCTCTGAAGATCAATGCTAAAGTTGGAGGATGCACGGTTTCCTTGTACAATTCGTTACCATCTCAAATCCCGCGGCTGCTTCTTGCTGATGAGCCAGTGATCTTCATGGGCGCAGATGTGACCCATCCACATCCACTTGATGACTTTAGTCCTTCTGTTGCTGCTGTGGTTGGTAGCATGAATTGGCCAGCGGCAAACAAGTATGTTTCGAGAATGAGATCTCAGACACATCGACAAGAAATCATCCAGGATCTCGACGCAATGGTGGAAGAATTATTGAATGAGTTTTGCCAGGAAGTTGGCAAACTCCCCAAGAGAATCATTTTCTTCAGAGACGGGGTAAGCGAAACGCAATTCTACAAAGTGCTTCAAGAGGAGCTGCAATCTATTAAAAGGGCATGTTCTAGGCTTCCCGGTTATGCGCCTCCCATAACATTTGCAGTGGTTCAGAAGAGGCATCACACAAGGCTGTTCCCTTTCAAAATTGATCCGTCTTCGAAGCAGAACCAATTACTCGATGAAAACATTCCCCCTGGAACCGTTGTGGATACCGTGATCACTCACCCGAAAGAATTCGACTTCTATCTTTGCAGTCATTGGGGAGTTAAGGGAACAAGCAGGCCAACTCATTACCACATTTTGCGGGATGAAAACGAGTTCACCTCAGATGAACTGCAGAAGCTGGTTAACATTCTGTGCTACACGTACGCAAGGTGCACGAAGCCGGTTTCGCTGGTGCCCCCAGCTTACTACGCTCACTTGGCAGCGTATCGAGGCAGGCTTTACCTTGAGAGATCAGAATCCACAACTTATACCAGAAGTGGTTCTACGCTCTCCAGAGCCGGCCCTCCGAAGGAAATGGCTCTACCAAAACTTAGTGAGAATGTTAAGAAACTCATGTTTTATTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009888 GO:0009892 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0019827 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035821 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043207 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0098542 GO:0098586 GO:0098727 GO:1901360 GO:1901698 GO:1901699

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

1023

Amino Acids

115.95

Weight (kDa)

9.39

Isoelectric Point (pI)

47.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	169 - 316	3.8e-28	N-terminal domain of argonaute
ArgoL1	PF08699	326 - 375	1.5e-15	Argonaute linker 1 domain
PAZ	PF02170	388 - 510	1.2e-27	PAZ domain
ArgoL2	PF16488	520 - 566	6.2e-07	Argonaute linker 2 domain
Piwi	PF02171	674 - 980	2.2e-92	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69440
fragaria_vesca	FvH4_4g30730
malus_domestica	MD13G1068200.v1.1 MD16G1067000.v1.1
prunus_persica	Prupe.1G279900_v2.0.a1
pyrus_communis	pycom13g06000 pycom16g05840
rosa_chinensis	RchiOBHm_Chr4g0439121
rosa_laevigata	RLG00000006296
rosa_roxburghii	Rroxscaffold_5G00380110
rosa_rugosa	Rorug04G0315900
rosa_samantha	Rh4AG366700 Rh4BG378200 Rh4CG392300 Rh4DG372800
rosa_wichuraiana	Rw0G007490 Rw0G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	5 cut(s)	68, 343, 955, 1823, 2893
AccB7I	CCANNNNNTGG	3 cut(s)	485, 934, 1610
AccII	CGCG	3 cut(s)	1293, 1660, 2230
AclWI	GGATC	4 cut(s)	1135, 1389, 2415, 2639
AcoI	YGGCCR	2 cut(s)	679, 2342
AcsI	RAATTY	7 cut(s)	707, 728, 1354, 1411, 1628, 1853, 2729
AcuI	CTGAAG	3 cut(s)	1341, 2183, 2470
AfaI	GTAC	4 cut(s)	1786, 2114, 2204, 2864
AfiI	CCNNNNNNNGG	6 cut(s)	410, 485, 598, 934, 1610, 3010
AflII	CTTAAG	1 cut(s)	1532
AflIII	ACRYGT	1 cut(s)	2859
AjnI	CCWGG	4 cut(s)	339, 2403, 2448, 2689
AloI	GAACNNNNNNTCC	2 cut(s)	2119, 2151
Alw21I	GWGCWC	2 cut(s)	1004, 2876
Alw26I	GTCTC	3 cut(s)	708, 1644, 2484
Alw44I	GTGCAC	1 cut(s)	2872
AlwI	GGATC	4 cut(s)	1135, 1389, 2415, 2639
AlwNI	CAGNNNCTG	3 cut(s)	485, 628, 2839
Ama87I	CYCGRG	4 cut(s)	997, 1163, 1496, 2123
AoxI	GGCC	8 cut(s)	476, 679, 1035, 1483, 1601, 2342, 2777, 3001
ApaLI	GTGCAC	1 cut(s)	2872
ApoI	RAATTY	7 cut(s)	707, 728, 1354, 1411, 1628, 1853, 2729
Asp700I	GAANNNNTTC	4 cut(s)	1278, 2682, 2729, 2980
AspLEI	GCGC	4 cut(s)	701, 1161, 2270, 2581
AspS9I	GGNCC	5 cut(s)	1035, 1592, 1601, 1723, 3002
AsuC2I	CCSGG	1 cut(s)	2571
AsuHPI	GGTGA	5 cut(s)	354, 393, 905, 2711, 2809
AsuII	TTCGAA	1 cut(s)	2654
AvaI	CYCGRG	4 cut(s)	997, 1163, 1496, 2123
AvaII	GGWCC	2 cut(s)	1592, 1723
AxyI	CCTNAGG	2 cut(s)	59, 915
BaeGI	GKGCMC	3 cut(s)	348, 2876, 2898
BalI	TGGCCA	1 cut(s)	2344
BanI	GGYRCC	5 cut(s)	68, 343, 955, 1823, 2893
BanII	GRGCYC	1 cut(s)	1004
BarI	GAAGNNNNNNTAC	4 cut(s)	677, 709, 2968, 3000
BauI	CACGAG	1 cut(s)	842
BbsI	GAAGAC	2 cut(s)	1199, 2643
Bbv12I	GWGCWC	2 cut(s)	1004, 2876
BceAI	ACGGC	1 cut(s)	1022
BciT130I	CCWGG	4 cut(s)	341, 2405, 2450, 2691
BciVI	GTATCC	2 cut(s)	1521, 2698
BclI	TGATCA	4 cut(s)	615, 1513, 1831, 2712
BcnI	CCSGG	1 cut(s)	2571
BcoDI	GTCTC	3 cut(s)	708, 1644, 2484
BfaI	CTAG	6 cut(s)	609, 854, 1088, 1788, 2156, 2562
BfmI	CTRYAG	2 cut(s)	372, 2831
BfoI	RGCGCY	2 cut(s)	702, 1162
BfrI	CTTAAG	1 cut(s)	1532
BfuI	GTATCC	2 cut(s)	1521, 2698
BglII	AGATCT	3 cut(s)	1324, 2059, 2375
Bme1390I	CCNGG	5 cut(s)	341, 2405, 2450, 2571, 2691
Bme18I	GGWCC	2 cut(s)	1592, 1723
BmeT110I	CYCGRG	4 cut(s)	997, 1163, 1496, 2123
BmgT120I	GGNCC	5 cut(s)	1035, 1592, 1601, 1723, 3002
BmiI	GGNNCC	8 cut(s)	70, 345, 957, 1036, 1724, 1825, 2695, 2895
BmrFI	CCNGG	5 cut(s)	341, 2405, 2450, 2571, 2691
BmrI	ACTGGG	1 cut(s)	1898
BmsI	GCATC	5 cut(s)	847, 1854, 2009, 2177, 2623
BmuI	ACTGGG	1 cut(s)	1898
BoxI	GACNNNNGTC	1 cut(s)	2304
BpiI	GAAGAC	2 cut(s)	1199, 2643
BplI	GAGNNNNNCTC	4 cut(s)	626, 658, 2804, 2836
BpmI	CTGGAG	3 cut(s)	269, 1997, 2977
Bpu14I	TTCGAA	1 cut(s)	2654
BpuEI	CTTGAG	3 cut(s)	967, 2048, 2966
BpuMI	CCSGG	1 cut(s)	2571
BsaAI	YACGTR	1 cut(s)	2862
BsaBI	GATNNNNATC	1 cut(s)	2956
BsaJI	CCNNGG	7 cut(s)	339, 933, 1308, 1495, 2122, 2228, 2689
BsaWI	WCCGGW	1 cut(s)	656
BsaXI	ACNNNNNCTCC	4 cut(s)	167, 197, 1023, 1053
Bsc4I	CCNNNNNNNGG	6 cut(s)	410, 485, 598, 934, 1610, 3010
Bse118I	RCCGGY	3 cut(s)	1746, 2881, 2999
Bse1I	ACTGG	5 cut(s)	136, 160, 396, 1893, 2252
Bse21I	CCTNAGG	2 cut(s)	59, 915
Bse3DI	GCAATG	2 cut(s)	767, 2424
Bse8I	GATNNNNATC	1 cut(s)	2956
BseBI	CCWGG	4 cut(s)	341, 2405, 2450, 2691
BseDI	CCNNGG	7 cut(s)	339, 933, 1308, 1495, 2122, 2228, 2689
BseJI	GATNNNNATC	1 cut(s)	2956
BseLI	CCNNNNNNNGG	6 cut(s)	410, 485, 598, 934, 1610, 3010
BseMI	GCAATG	2 cut(s)	767, 2424
BseMII	CTCAG	6 cut(s)	50, 929, 1037, 1200, 2393, 2835
BseNI	ACTGG	5 cut(s)	136, 160, 396, 1893, 2252
BseRI	GAGGAG	9 cut(s)	169, 172, 201, 206, 303, 319, 734, 1044, 2546
BseSI	GKGCMC	3 cut(s)	348, 2876, 2898
BseYI	CCCAGC	2 cut(s)	190, 2899
Bsh1236I	CGCG	3 cut(s)	1293, 1660, 2230
Bsh1285I	CGRYCG	1 cut(s)	1553
BshFI	GGCC	8 cut(s)	478, 681, 1037, 1485, 1603, 2344, 2779, 3003
BshNI	GGYRCC	5 cut(s)	68, 343, 955, 1823, 2893
BsiEI	CGRYCG	1 cut(s)	1553
BsiHKAI	GWGCWC	2 cut(s)	1004, 2876
BsiHKCI	CYCGRG	4 cut(s)	997, 1163, 1496, 2123
BsiSI	CCGG	7 cut(s)	657, 668, 682, 1747, 2571, 2882, 3000
BsiWI	CGTACG	1 cut(s)	2862
BslFI	GGGAC	2 cut(s)	514, 1736
BslI	CCNNNNNNNGG	6 cut(s)	410, 485, 598, 934, 1610, 3010
BsmAI	GTCTC	3 cut(s)	708, 1644, 2484
BsmBI	CGTCTC	2 cut(s)	708, 2484
BsmFI	GGGAC	2 cut(s)	514, 1736
BsmI	GAATGC	3 cut(s)	782, 1118, 1614
BsnI	GGCC	8 cut(s)	478, 681, 1037, 1485, 1603, 2344, 2779, 3003
BsoBI	CYCGRG	4 cut(s)	997, 1163, 1496, 2123
Bsp119I	TTCGAA	1 cut(s)	2654
Bsp1286I	GDGCHC	4 cut(s)	348, 1004, 2876, 2898
Bsp1407I	TGTACA	1 cut(s)	2202
BspANI	GGCC	8 cut(s)	478, 681, 1037, 1485, 1603, 2344, 2779, 3003
BspCNI	CTCAG	6 cut(s)	51, 928, 1038, 1201, 2392, 2834
BspFNI	CGCG	3 cut(s)	1293, 1660, 2230
BspLI	GGNNCC	8 cut(s)	70, 345, 957, 1036, 1724, 1825, 2695, 2895
BspMAI	CTGCAG	1 cut(s)	2835
BspPI	GGATC	4 cut(s)	1135, 1389, 2415, 2639
BspQI	GCTCTTC	1 cut(s)	201
BspT104I	TTCGAA	1 cut(s)	2654
BspT107I	GGYRCC	5 cut(s)	68, 343, 955, 1823, 2893
BspTI	CTTAAG	1 cut(s)	1532
BsrDI	GCAATG	2 cut(s)	767, 2424
BsrFI	RCCGGY	3 cut(s)	1746, 2881, 2999
BsrGI	TGTACA	1 cut(s)	2202
BsrI	ACTGG	5 cut(s)	136, 160, 396, 1893, 2252
BssAI	RCCGGY	3 cut(s)	1746, 2881, 2999
BssECI	CCNNGG	7 cut(s)	339, 933, 1308, 1495, 2122, 2228, 2689
BssSI	CACGAG	1 cut(s)	842
BssT1I	CCWWGG	1 cut(s)	933
Bst2BI	CACGAG	1 cut(s)	842
Bst2UI	CCWGG	4 cut(s)	341, 2405, 2450, 2691
Bst4CI	ACNGT	5 cut(s)	505, 1279, 2194, 2698, 2710
Bst6I	CTCTTC	3 cut(s)	201, 1666, 2603
BstAFI	CTTAAG	1 cut(s)	1532
BstAUI	TGTACA	1 cut(s)	2202
BstBAI	YACGTR	1 cut(s)	2862
BstBI	TTCGAA	1 cut(s)	2654
BstC8I	GCNNGC	6 cut(s)	302, 1865, 2346, 2777, 2936, 3001
BstDEI	CTNAG	8 cut(s)	59, 915, 1046, 1209, 1974, 2379, 2821, 3032
BstDSI	CCRYGG	1 cut(s)	2228
BstFNI	CGCG	3 cut(s)	1293, 1660, 2230
BstH2I	RGCGCY	2 cut(s)	702, 1162
BstHHI	GCGC	4 cut(s)	701, 1161, 2270, 2581
BstMAI	GTCTC	3 cut(s)	708, 1644, 2484
BstMCI	CGRYCG	1 cut(s)	1553
BstMWI	GCNNNNNNNGC	9 cut(s)	668, 769, 1299, 1423, 2106, 2156, 2887, 2909, 2918
BstNI	CCWGG	4 cut(s)	341, 2405, 2450, 2691
BstNSI	RCATGY	1 cut(s)	2559
BstPAI	GACNNNNGTC	1 cut(s)	2304
BstSCI	CCNGG	5 cut(s)	339, 2403, 2448, 2569, 2689
BstSFI	CTRYAG	2 cut(s)	372, 2831
BstSLI	GKGCMC	3 cut(s)	348, 2876, 2898
BstUI	CGCG	3 cut(s)	1293, 1660, 2230
BstV2I	GAAGAC	2 cut(s)	1199, 2643
BstX2I	RGATCY	4 cut(s)	1324, 2059, 2375, 2407
BstXI	CCANNNNNNTGG	1 cut(s)	2456
BstYI	RGATCY	4 cut(s)	1324, 2059, 2375, 2407
Bsu36I	CCTNAGG	2 cut(s)	59, 915
BsuI	GTATCC	2 cut(s)	1521, 2698
BsuRI	GGCC	8 cut(s)	478, 681, 1037, 1485, 1603, 2344, 2779, 3003
BtgI	CCRYGG	1 cut(s)	2228
BtgZI	GCGATG	2 cut(s)	534, 1719
BtsI	GCAGTG	2 cut(s)	1757, 2604
BtsIMutI	CAGTG	4 cut(s)	1317, 1757, 2259, 2604
Cac8I	GCNNGC	6 cut(s)	302, 1865, 2346, 2777, 2936, 3001
CaiI	CAGNNNCTG	3 cut(s)	485, 628, 2839
CfoI	GCGC	4 cut(s)	701, 1161, 2270, 2581
Cfr10I	RCCGGY	3 cut(s)	1746, 2881, 2999
Cfr13I	GGNCC	5 cut(s)	1035, 1592, 1601, 1723, 3002
Cfr42I	CCGCGG	1 cut(s)	2231
CseI	GACGC	2 cut(s)	1666, 2423
Csp6I	GTAC	4 cut(s)	1785, 2113, 2203, 2863
CviQI	GTAC	4 cut(s)	1785, 2113, 2203, 2863
DdeI	CTNAG	8 cut(s)	59, 915, 1046, 1209, 1974, 2379, 2821, 3032
EaeI	YGGCCR	2 cut(s)	679, 2342
Eam1104I	CTCTTC	3 cut(s)	201, 1666, 2603
EarI	CTCTTC	3 cut(s)	201, 1666, 2603
Ecl136II	GAGCTC	1 cut(s)	1002
Eco130I	CCWWGG	1 cut(s)	933
Eco24I	GRGCYC	1 cut(s)	1004
Eco47I	GGWCC	2 cut(s)	1592, 1723
Eco53kI	GAGCTC	1 cut(s)	1002
Eco57I	CTGAAG	3 cut(s)	1341, 2183, 2470
Eco81I	CCTNAGG	2 cut(s)	59, 915
Eco88I	CYCGRG	4 cut(s)	997, 1163, 1496, 2123
EcoICRI	GAGCTC	1 cut(s)	1002
EcoO109I	RGGNCCY	2 cut(s)	1592, 1723
EcoRI	GAATTC	4 cut(s)	707, 728, 1628, 2729
EcoRII	CCWGG	4 cut(s)	339, 2403, 2448, 2689
EcoT14I	CCWWGG	1 cut(s)	933
EcoT38I	GRGCYC	1 cut(s)	1004
ErhI	CCWWGG	1 cut(s)	933
Esp3I	CGTCTC	2 cut(s)	708, 2484
FalI	AAGNNNNNCTT	2 cut(s)	789, 821
FaqI	GGGAC	2 cut(s)	514, 1736
FauI	CCCGC	2 cut(s)	2235, 2793
FauNDI	CATATG	1 cut(s)	775
FbaI	TGATCA	4 cut(s)	615, 1513, 1831, 2712
FriOI	GRGCYC	1 cut(s)	1004
FspBI	CTAG	6 cut(s)	609, 854, 1088, 1788, 2156, 2562
GlaI	GCGC	4 cut(s)	700, 1160, 2269, 2580
GsaI	CCCAGC	2 cut(s)	194, 2903
GsuI	CTGGAG	3 cut(s)	269, 1997, 2977
HaeII	RGCGCY	2 cut(s)	702, 1162
HaeIII	GGCC	8 cut(s)	478, 681, 1037, 1485, 1603, 2344, 2779, 3003
HapII	CCGG	7 cut(s)	657, 668, 682, 1747, 2571, 2882, 3000
HgaI	GACGC	2 cut(s)	1666, 2423
HhaI	GCGC	4 cut(s)	701, 1161, 2270, 2581
Hin6I	GCGC	4 cut(s)	699, 1159, 2268, 2579
HinP1I	GCGC	4 cut(s)	699, 1159, 2268, 2579
HincII	GTYRAC	3 cut(s)	577, 1368, 2845
HindII	GTYRAC	3 cut(s)	577, 1368, 2845
HindIII	AAGCTT	2 cut(s)	803, 1697
HpaI	GTTAAC	1 cut(s)	2845
HpaII	CCGG	7 cut(s)	657, 668, 682, 1747, 2571, 2882, 3000
HphI	GGTGA	5 cut(s)	354, 393, 905, 2711, 2809
Hpy166II	GTNNAC	8 cut(s)	401, 577, 1368, 1442, 1883, 2817, 2845, 2874
Hpy8I	GTNNAC	8 cut(s)	401, 577, 1368, 1442, 1883, 2817, 2845, 2874
Hpy99I	CGWCG	1 cut(s)	2417
HpyCH4III	ACNGT	5 cut(s)	505, 1279, 2194, 2698, 2710
HpyCH4IV	ACGT	1 cut(s)	2861
HpyF10VI	GCNNNNNNNGC	9 cut(s)	668, 769, 1299, 1423, 2106, 2156, 2887, 2909, 2918
HpyF3I	CTNAG	8 cut(s)	59, 915, 1046, 1209, 1974, 2379, 2821, 3032
HpySE526I	ACGT	1 cut(s)	2861
HspAI	GCGC	4 cut(s)	699, 1159, 2268, 2579
KroI	GCCGGC	1 cut(s)	2999
KroNI	GCCGGC	1 cut(s)	3001
Ksp22I	TGATCA	4 cut(s)	615, 1513, 1831, 2712
KspAI	GTTAAC	1 cut(s)	2845
KspI	CCGCGG	1 cut(s)	2231
LguI	GCTCTTC	1 cut(s)	201
LmnI	GCTCC	3 cut(s)	1462, 1641, 2533
LweI	GCATC	5 cut(s)	847, 1854, 2009, 2177, 2623
MaeI	CTAG	6 cut(s)	609, 854, 1088, 1788, 2156, 2562
MaeII	ACGT	1 cut(s)	2861
MaeIII	GTNAC	5 cut(s)	463, 875, 1111, 2211, 2275
MflI	RGATCY	4 cut(s)	1324, 2059, 2375, 2407
MhlI	GDGCHC	4 cut(s)	348, 1004, 2876, 2898
MlsI	TGGCCA	1 cut(s)	2344
MluNI	TGGCCA	1 cut(s)	2344
MmeI	TCCRAC	3 cut(s)	1114, 1957, 2161
Mox20I	TGGCCA	1 cut(s)	2344
MroNI	GCCGGC	1 cut(s)	2999
MroXI	GAANNNNTTC	4 cut(s)	1278, 2682, 2729, 2980
MscI	TGGCCA	1 cut(s)	2344
MslI	CAYNNNNRTG	1 cut(s)	570
Msp20I	TGGCCA	1 cut(s)	2344
MspA1I	CMGCKG	5 cut(s)	247, 440, 2017, 2230, 2348
MspCI	CTTAAG	1 cut(s)	1532
MspI	CCGG	7 cut(s)	657, 668, 682, 1747, 2571, 2882, 3000
MspR9I	CCNGG	5 cut(s)	341, 2405, 2450, 2571, 2691
Mva1269I	GAATGC	3 cut(s)	782, 1118, 1614
MvaI	CCWGG	4 cut(s)	341, 2405, 2450, 2691
MvnI	CGCG	3 cut(s)	1293, 1660, 2230
MwoI	GCNNNNNNNGC	9 cut(s)	668, 769, 1299, 1423, 2106, 2156, 2887, 2909, 2918
NaeI	GCCGGC	1 cut(s)	3001
NciI	CCSGG	1 cut(s)	2571
NdeI	CATATG	1 cut(s)	775
NgoMIV	GCCGGC	1 cut(s)	2999
NlaIV	GGNNCC	8 cut(s)	70, 345, 957, 1036, 1724, 1825, 2695, 2895
NmeAIII	GCCGAG	1 cut(s)	623
NmuCI	GTSAC	3 cut(s)	463, 1111, 2275
NspI	RCATGY	1 cut(s)	2559
NspV	TTCGAA	1 cut(s)	2654
PaeR7I	CTCGAG	2 cut(s)	997, 1163
PciSI	GCTCTTC	1 cut(s)	201
PcsI	WCGNNNNNNNCGW	1 cut(s)	2499
PctI	GAATGC	3 cut(s)	782, 1118, 1614
PdiI	GCCGGC	1 cut(s)	3001
PdmI	GAANNNNTTC	4 cut(s)	1278, 2682, 2729, 2980
Pfl23II	CGTACG	1 cut(s)	2862
PflMI	CCANNNNNTGG	3 cut(s)	485, 934, 1610
PfoI	TCCNGGA	1 cut(s)	2403
Ppu21I	YACGTR	1 cut(s)	2862
PpuMI	RGGWCCY	2 cut(s)	1592, 1723
PshAI	GACNNNNGTC	1 cut(s)	2304
Psp124BI	GAGCTC	1 cut(s)	1004
Psp5II	RGGWCCY	2 cut(s)	1592, 1723
Psp6I	CCWGG	4 cut(s)	339, 2403, 2448, 2689
PspFI	CCCAGC	2 cut(s)	190, 2899
PspGI	CCWGG	4 cut(s)	339, 2403, 2448, 2689
PspLI	CGTACG	1 cut(s)	2862
PspN4I	GGNNCC	8 cut(s)	70, 345, 957, 1036, 1724, 1825, 2695, 2895
PspPI	GGNCC	5 cut(s)	1035, 1592, 1601, 1723, 3002
PspPPI	RGGWCCY	2 cut(s)	1592, 1723
PspXI	VCTCGAGB	2 cut(s)	997, 1163
PstI	CTGCAG	1 cut(s)	2835
PstNI	CAGNNNCTG	3 cut(s)	485, 628, 2839
PsuI	RGATCY	4 cut(s)	1324, 2059, 2375, 2407
PvuII	CAGCTG	2 cut(s)	440, 2017
RsaI	GTAC	4 cut(s)	1786, 2114, 2204, 2864
RsaNI	GTAC	4 cut(s)	1785, 2113, 2203, 2863
RseI	CAYNNNNRTG	1 cut(s)	570
SacI	GAGCTC	1 cut(s)	1004
SacII	CCGCGG	1 cut(s)	2231
SapI	GCTCTTC	1 cut(s)	201
Sau96I	GGNCC	5 cut(s)	1035, 1592, 1601, 1723, 3002
ScrFI	CCNGG	5 cut(s)	341, 2405, 2450, 2571, 2691
SduI	GDGCHC	4 cut(s)	348, 1004, 2876, 2898
SfaNI	GCATC	5 cut(s)	847, 1854, 2009, 2177, 2623
SfcI	CTRYAG	2 cut(s)	372, 2831
Sfr274I	CTCGAG	2 cut(s)	997, 1163
Sfr303I	CCGCGG	1 cut(s)	2231
SfuI	TTCGAA	1 cut(s)	2654
SgrBI	CCGCGG	1 cut(s)	2231
SinI	GGWCC	2 cut(s)	1592, 1723
SlaI	CTCGAG	2 cut(s)	997, 1163
SmiMI	CAYNNNNRTG	1 cut(s)	570
SmlI	CTYRAG	6 cut(s)	946, 997, 1163, 1532, 2063, 2945
SmoI	CTYRAG	6 cut(s)	946, 997, 1163, 1532, 2063, 2945
SspI	AATATT	1 cut(s)	561
SspMI	CTAG	6 cut(s)	609, 854, 1088, 1788, 2156, 2562
SstI	GAGCTC	1 cut(s)	1004
StyD4I	CCNGG	5 cut(s)	339, 2403, 2448, 2569, 2689
StyI	CCWWGG	1 cut(s)	933
TaaI	ACNGT	5 cut(s)	505, 1279, 2194, 2698, 2710
TaiI	ACGT	1 cut(s)	2864
TaqII	GACCGA	1 cut(s)	1567
TatI	WGTACW	2 cut(s)	2112, 2202
TauI	GCSGC	5 cut(s)	69, 326, 910, 2233, 2351
TscAI	CASTG	4 cut(s)	1324, 1757, 2259, 2604
TseFI	GTSAC	3 cut(s)	463, 1111, 2275
Tsp45I	GTSAC	3 cut(s)	463, 1111, 2275
TspDTI	ATGAA	9 cut(s)	285, 537, 912, 1846, 2251, 2351, 2691, 2820, 2841
TspGWI	ACGGA	2 cut(s)	1099, 2637
TspRI	CASTG	4 cut(s)	1324, 1757, 2259, 2604
Van91I	CCANNNNNTGG	3 cut(s)	485, 934, 1610
Vha464I	CTTAAG	1 cut(s)	1532
VneI	GTGCAC	1 cut(s)	2872
VpaK11BI	GGWCC	2 cut(s)	1592, 1723
XapI	RAATTY	7 cut(s)	707, 728, 1354, 1411, 1628, 1853, 2729
XceI	RCATGY	1 cut(s)	2559
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1130
XhoI	CTCGAG	2 cut(s)	997, 1163
XmnI	GAANNNNTTC	4 cut(s)	1278, 2682, 2729, 2980
XspI	CTAG	6 cut(s)	609, 854, 1088, 1788, 2156, 2562

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.