Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0014403

Physical Location & Seq

Genomic Coordinates Reverse (-)

64235857 .. 64239445

Gene Structure 3589 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG392300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3063 bp

ATGGAAGAGACAGAAGAGTCCAATGGTAACAAGAAATGCAACACCAAAATCATAAGGGGCAGAACCAACAACCCACCTCAGAAGCACCATCACCATCACCATCACCATCACCAGCTAATACAATACTCAAATCAGTTTGGGTTCTTCAACCAGAACCAGTACTATCAGAGATACTACTATCCAGCTCTTCTTCCCCTCCCTCCTCTTATACCTCTGCCTCTGGCTTTAACTCCACCCTTGCCTCAGAACCACAGCTTCAGAACAAAAACCCATTTGCAGAAACCTTCATGCAAGCACAACAACTCTCCTTTTGCTGCTTCCTCAGAAACCCAAGTGCCCAAAGTCTCGATTTCACCAGAAGCTTCAAAAGGGTTTCAAAAGCAAACCAATTTGCCCCTTAAAGGAGAAAATGGAAGGAAATTCACAAGTGCAAGAACAGGGAAAGCAATAGTGACAGCAAAGAGGCCAGACTATGGTGGGGTGGAAGGGACTCCTATTTCTCTCTTAGCTAACCATTTCCTTGTCCAATTCAATTCATCGGAGCGAATTTTTCACTACAATGTTGAAATCTCTCCTAGTCCCTCCAAGGACATTTCTCGAATGATCAAGCAAAAACTGGTGGAGGATAACTCAGCTTTGCTCTGTGGTGCTAGTCCGGCTTATGATGGCCGCAAGAATCTTTACAGCTCAATCGAATTCCAACATGATAAGCTTGAAATGTATGTAAGCCTCCCCATCCCTACAACCAAGCCAACCTTGCCATGTGGGGAATTTGGTGACTTTCAAGAGAAGGATCATCAAATACTTAAACTTTTTCGTGTAAGCATCAAACTGGTTTCGAAGTTTGATGGAAAGGAATTGAGTAGCTACTTGGGCAAGGATGGAGATGAGTGGAAACCTATCCCTCAGGATTATCTTCATGCCTTGGATGTCGTTTTGAGAGAGAATCCATTGGAGAAGTGTGTGCCTGTGGGAAGATCACTTTATTCCAGTTCAATGGGAGGAACTAAAGACATTGGAGGAGGAGCTGTAGGATATAGAGGGTTCTTTCAGAGCCTTAGACCAACCCAACAAGGACTAGCTCTCAATGTTGATTTCTCCGTGACCGCTTTCCATGAAAGCATTGGAATGATCCCATACTTGCAGAAGCGTCTCGAGTTTCTTCGAGACCTTGCTCAGAGGAAGACAAGGGGTTTAAGTAGTGAAGAAAGGAAAGAAGTGGAGAAGGCATTGAAGAACATCAGGGTCTTTGTTTGCCATAGAGAAACAGTTCAGAGATACCGAGTTTTTGGCTTAACCGAGGAAGCCACTGAGAATCTTTGGTTTCCAGACAGGGATGGGAAGAATTTGAGGCTGCTGACTTACTTCAAGGATCACTACAACTATGATATACAGTTCAGGAATTTGCCATGTTTGCAGATTAGTAGGAGTAAACCATGTTATCTTCCTATGGAGCTATGTATGATCTGTGAAGGCCAGAAGTTCCTTGGGAAGCTTTCAGATGATCAGACTGCAAGGATACTTAAGATGGGCTGCCAAGGACCGAAAGAACGAAAATCCATCATAGATGGAGTCATGAGAGGACCTGTTGGGCCAATAAGTGGCATTCAGGAAAGGGAATTCAAACTCCGTGTTTCTAGAGAAATGACGCGATTAAATGGAAGAATTCTTCAGCCTCCGAAGCTAAAGCTTGGCGATGGTGGCCATGTAACAGACCTCATTCCTTCCCGTCATGATCGGCAGTGGAATCTTCTGGACAGCCATGTGTTTGAAGGAACTTGCGTTGAGAGGTGGGCATTGATTAGTTTTGGTGGCACCCCAGAACAGAAGCACTGCATTCCCAAATTCATACACCAGCTATCTCAAAGATGTGAACAACTAGGCATCTTTCTTAACAAAACCACAATTGTTAGCCCCCAATTCGAACCATCCCAAGTGCTTAACAATGTATCTCTTTTAGAATCTAAGATTAAGAAAATCCAAAGAGCTGCATCAAACAATCTCCAGCTGCTTATCTGTGTAATGGAAAGAAAACACAAAGGGTATGCAGACCTGAAGCGAATTGCGGATACAGGCGTTGGTGTTCTAACACAATGCTGCTTGTACTCAAACCTTTGCAGATTGAGTTCACAGTTTTTGGCTAACTTGGCTCTCAAGATCAATGCCAAAGTTGGAGGATGCACAGTTTCTTTGTACAACTCTTTGCCATCTCAAATCCCCCGGCTGCTTCAGAGTGATGAGCCAGTCATCTTTATGGGTGCTGATGTTACTCATCCTCACCCACTCGATGATTTCAGCCCTTCTGTTGCTGCTGTGGTTGGTAGCATGAATTGGCCTGCAGCAAACAAGTATGTTTCAAGAATGAGGTCACAAACACATAGGCAAGAAATCATTGAGGATCTTGGTGCAATGGTGGAAGAATTGTTGGATGAGTTTCACCAAGAAGTAGGCAAACTCCCAAAGAGAATAGTTTTCTTCAGAGATGGGGTAAGCGAAACACAATTCTACAAAGTGCTTCATGAAGAATTGCAAGCCATTAAAAAAGGTTGTTCTAAATTCCCTGGTTACACACCTCCCATAACCTTTGCAGTGGTTCAGAAGAGGCATCACACAAGATTGTTCCCTTTCAAAATTGATCCGTCTTCCAAACAGAACCAGTTCCTGGACGAAAACATTCCCCCGGGAACTGTGGTGGACACGGTGATCACTCACCCGAGAGAGTTTGATTTCTATCTTTGTAGCCATTGGGGTGTTAAGGGAACAAGCCGGCCAACACATTACCACATTCTGTGGGATGAAAACGAGTTCACTTCAGATGAACTGCAGAAACTGGTGAACATTCTATGCTACACATACGTAAGGTGCACCAAGCCGGTTTCTCTGGTGCCCCCGGCTTATTATGCTCACCTAGCTGCGTATAGAGGAAGGCTTTACCTTGAGCGATCGGAATCCACAACAGCTTACACAAGAAGTGGCTCCATACTCTCCAGAGCTGCCCCTCCAAAGGCAATGGCTCTACCAAAACTTAGTGAGAATGTTAGGAAGCTTATGTTTTACTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009888 GO:0009892 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0019827 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035821 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043207 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0098542 GO:0098586 GO:0098727 GO:1901360 GO:1901698 GO:1901699

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

1020

Amino Acids

116.04

Weight (kDa)

9.23

Isoelectric Point (pI)

43.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	164 - 313	6.3e-28	N-terminal domain of argonaute
ArgoL1	PF08699	323 - 372	7.8e-17	Argonaute linker 1 domain
PAZ	PF02170	385 - 507	4.1e-28	PAZ domain
ArgoL2	PF16488	517 - 563	1.7e-06	Argonaute linker 2 domain
Piwi	PF02171	671 - 977	7.9e-91	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69440
fragaria_vesca	FvH4_4g30730
malus_domestica	MD13G1068200.v1.1 MD16G1067000.v1.1
prunus_persica	Prupe.1G279900_v2.0.a1
pyrus_communis	pycom13g06000 pycom16g05840
rosa_chinensis	RchiOBHm_Chr4g0439121
rosa_laevigata	RLG00000006296
rosa_roxburghii	Rroxscaffold_5G00380110
rosa_rugosa	Rorug04G0315900
rosa_samantha	Rh4AG366700 Rh4BG378200 Rh4CG392300 Rh4DG372800
rosa_wichuraiana	Rw0G007490 Rw0G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	16
AccB1I	GGYRCC	2 cut(s)	1814, 2884
AccB7I	CCANNNNNTGG	3 cut(s)	473, 1601, 2662
AccII	CGCG	1 cut(s)	1651
AciI	CCGC	3 cut(s)	670, 1107, 2066
AclWI	GGATC	5 cut(s)	801, 1126, 1380, 2406, 2630
AcoI	YGGCCR	3 cut(s)	667, 1702, 2768
AcuI	CTGAAG	6 cut(s)	241, 1655, 2075, 2213, 2461, 2796
AfaI	GTAC	3 cut(s)	161, 2105, 2195
AfiI	CCNNNNNNNGG	5 cut(s)	401, 473, 1601, 2662, 3004
AflII	CTTAAG	1 cut(s)	1523
AjnI	CCWGG	2 cut(s)	2560, 2661
Alw21I	GWGCWC	1 cut(s)	2867
Alw26I	GTCTC	4 cut(s)	2, 349, 1157, 1161
Alw44I	GTGCAC	1 cut(s)	2863
AlwI	GGATC	5 cut(s)	801, 1126, 1380, 2406, 2630
AlwNI	CAGNNNCTG	2 cut(s)	2662, 2830
Ama87I	CYCGRG	3 cut(s)	1154, 2680, 2713
AoxI	GGCC	7 cut(s)	464, 667, 1474, 1592, 1702, 2333, 2768
ApaLI	GTGCAC	1 cut(s)	2863
Asp700I	GAANNNNTTC	3 cut(s)	1269, 2657, 2673
AspS9I	GGNCC	3 cut(s)	1541, 1583, 1592
AsuC2I	CCSGG	4 cut(s)	2221, 2681, 2682, 2891
AsuII	TTCGAA	2 cut(s)	839, 1923
AvaI	CYCGRG	3 cut(s)	1154, 2680, 2713
AvaII	GGWCC	2 cut(s)	1541, 1583
AxyI	CCTNAGG	1 cut(s)	906
BaeGI	GKGCMC	3 cut(s)	339, 2867, 2889
BaeI	ACNNNNGTAYC	2 cut(s)	2061, 2094
BalI	TGGCCA	1 cut(s)	1704
BanI	GGYRCC	2 cut(s)	1814, 2884
BbsI	GAAGAC	2 cut(s)	1190, 2634
Bbv12I	GWGCWC	1 cut(s)	2867
BciT130I	CCWGG	2 cut(s)	2562, 2663
BciVI	GTATCC	2 cut(s)	1512, 2062
BclI	TGATCA	3 cut(s)	603, 1504, 2703
BcnI	CCSGG	4 cut(s)	2221, 2681, 2682, 2891
BcoDI	GTCTC	4 cut(s)	2, 349, 1157, 1161
BfaI	CTAG	6 cut(s)	576, 651, 1079, 1638, 1880, 2909
BfmI	CTRYAG	3 cut(s)	1029, 2337, 2822
BfrI	CTTAAG	1 cut(s)	1523
BfuI	GTATCC	2 cut(s)	1512, 2062
BmcAI	AGTACT	1 cut(s)	161
Bme1390I	CCNGG	6 cut(s)	2221, 2562, 2663, 2681, 2682, 2891
Bme18I	GGWCC	2 cut(s)	1541, 1583
BmeT110I	CYCGRG	3 cut(s)	1154, 2680, 2713
BmgT120I	GGNCC	3 cut(s)	1541, 1583, 1592
BmiI	GGNNCC	3 cut(s)	1816, 2886, 2977
BmrFI	CCNGG	6 cut(s)	2221, 2562, 2663, 2681, 2682, 2891
BmsI	GCATC	5 cut(s)	834, 1893, 2000, 2168, 2614
BpiI	GAAGAC	2 cut(s)	1190, 2634
BplI	GAGNNNNNCTC	2 cut(s)	614, 646
BpmI	CTGGAG	2 cut(s)	1988, 2971
Bpu14I	TTCGAA	2 cut(s)	839, 1923
BpuEI	CTTGAG	2 cut(s)	2138, 2957
BpuMI	CCSGG	4 cut(s)	2221, 2681, 2682, 2891
BsaAI	YACGTR	1 cut(s)	2857
BsaBI	GATNNNNATC	2 cut(s)	2730, 2947
BsaI	GGTCTC	1 cut(s)	1161
BsaXI	ACNNNNNCTCC	2 cut(s)	1014, 1044
Bsc4I	CCNNNNNNNGG	5 cut(s)	401, 473, 1601, 2662, 3004
Bse118I	RCCGGY	2 cut(s)	2766, 2872
Bse1I	ACTGG	7 cut(s)	157, 621, 837, 990, 2243, 2656, 2835
Bse21I	CCTNAGG	1 cut(s)	906
Bse3DI	GCAATG	2 cut(s)	2415, 3015
Bse8I	GATNNNNATC	2 cut(s)	2730, 2947
BseBI	CCWGG	2 cut(s)	2562, 2663
BseGI	GGATG	9 cut(s)	735, 886, 934, 1342, 1928, 2183, 2272, 2434, 2800
BseJI	GATNNNNATC	2 cut(s)	2730, 2947
BseLI	CCNNNNNNNGG	5 cut(s)	401, 473, 1601, 2662, 3004
BseMI	GCAATG	2 cut(s)	2415, 3015
BseMII	CTCAG	7 cut(s)	92, 257, 336, 645, 920, 1190, 1302
BseNI	ACTGG	7 cut(s)	157, 621, 837, 990, 2243, 2656, 2835
BseRI	GAGGAG	3 cut(s)	192, 1035, 1038
BseSI	GKGCMC	3 cut(s)	339, 2867, 2889
Bsh1236I	CGCG	1 cut(s)	1651
Bsh1285I	CGRYCG	1 cut(s)	2945
BshFI	GGCC	7 cut(s)	466, 669, 1476, 1594, 1704, 2335, 2770
BshNI	GGYRCC	2 cut(s)	1814, 2884
BsiEI	CGRYCG	1 cut(s)	2945
BsiHKAI	GWGCWC	1 cut(s)	2867
BsiHKCI	CYCGRG	3 cut(s)	1154, 2680, 2713
BsiSI	CCGG	6 cut(s)	656, 2221, 2681, 2767, 2873, 2891
BslFI	GGGAC	2 cut(s)	502, 564
BslI	CCNNNNNNNGG	5 cut(s)	401, 473, 1601, 2662, 3004
BsmAI	GTCTC	4 cut(s)	2, 349, 1157, 1161
BsmBI	CGTCTC	1 cut(s)	1157
BsmFI	GGGAC	2 cut(s)	502, 564
BsmI	GAATGC	2 cut(s)	1605, 1836
BsnI	GGCC	7 cut(s)	466, 669, 1476, 1594, 1704, 2335, 2770
Bso31I	GGTCTC	1 cut(s)	1161
BsoBI	CYCGRG	3 cut(s)	1154, 2680, 2713
Bsp119I	TTCGAA	2 cut(s)	839, 1923
Bsp1286I	GDGCHC	3 cut(s)	339, 2867, 2889
Bsp1407I	TGTACA	1 cut(s)	2193
BspACI	CCGC	3 cut(s)	670, 1107, 2066
BspANI	GGCC	7 cut(s)	466, 669, 1476, 1594, 1704, 2335, 2770
BspCNI	CTCAG	7 cut(s)	91, 256, 335, 644, 919, 1189, 1303
BspFNI	CGCG	1 cut(s)	1651
BspHI	TCATGA	3 cut(s)	1575, 1732, 2518
BspLI	GGNNCC	3 cut(s)	1816, 2886, 2977
BspMAI	CTGCAG	2 cut(s)	2341, 2826
BspPI	GGATC	5 cut(s)	801, 1126, 1380, 2406, 2630
BspQI	GCTCTTC	1 cut(s)	192
BspT104I	TTCGAA	2 cut(s)	839, 1923
BspT107I	GGYRCC	2 cut(s)	1814, 2884
BspTI	CTTAAG	1 cut(s)	1523
BspTNI	GGTCTC	1 cut(s)	1161
BsrDI	GCAATG	2 cut(s)	2415, 3015
BsrFI	RCCGGY	2 cut(s)	2766, 2872
BsrGI	TGTACA	1 cut(s)	2193
BsrI	ACTGG	7 cut(s)	157, 621, 837, 990, 2243, 2656, 2835
BssAI	RCCGGY	2 cut(s)	2766, 2872
BssT1I	CCWWGG	4 cut(s)	585, 924, 1486, 1537
Bst2UI	CCWGG	2 cut(s)	2562, 2663
Bst4CI	ACNGT	6 cut(s)	1270, 1395, 2133, 2185, 2689, 2701
Bst6I	CTCTTC	3 cut(s)	9, 192, 2594
BstAFI	CTTAAG	1 cut(s)	1523
BstAUI	TGTACA	1 cut(s)	2193
BstBAI	YACGTR	1 cut(s)	2857
BstBI	TTCGAA	2 cut(s)	839, 1923
BstC8I	GCNNGC	4 cut(s)	293, 2337, 2532, 2768
BstF5I	GGATG	9 cut(s)	735, 886, 934, 1342, 1928, 2183, 2272, 2434, 2800
BstFNI	CGCG	1 cut(s)	1651
BstMAI	GTCTC	4 cut(s)	2, 349, 1157, 1161
BstMCI	CGRYCG	1 cut(s)	2945
BstMWI	GCNNNNNNNGC	9 cut(s)	656, 757, 873, 1414, 1681, 1701, 2147, 2900, 2909
BstNI	CCWGG	2 cut(s)	2562, 2663
BstSCI	CCNGG	6 cut(s)	2219, 2560, 2661, 2679, 2680, 2889
BstSFI	CTRYAG	3 cut(s)	1029, 2337, 2822
BstSLI	GKGCMC	3 cut(s)	339, 2867, 2889
BstSNI	TACGTA	1 cut(s)	2857
BstUI	CGCG	1 cut(s)	1651
BstV2I	GAAGAC	2 cut(s)	1190, 2634
BstX2I	RGATCY	1 cut(s)	2398
BstXI	CCANNNNNNTGG	1 cut(s)	997
BstYI	RGATCY	1 cut(s)	2398
Bsu36I	CCTNAGG	1 cut(s)	906
BsuI	GTATCC	2 cut(s)	1512, 2062
BsuRI	GGCC	7 cut(s)	466, 669, 1476, 1594, 1704, 2335, 2770
BtgZI	GCGATG	1 cut(s)	1710
BtsCI	GGATG	9 cut(s)	735, 886, 934, 1342, 1928, 2183, 2272, 2434, 2800
BtsI	GCAGTG	3 cut(s)	1748, 1831, 2595
BtsIMutI	CAGTG	4 cut(s)	1308, 1748, 1831, 2595
Cac8I	GCNNGC	4 cut(s)	293, 2337, 2532, 2768
CaiI	CAGNNNCTG	2 cut(s)	2662, 2830
CciI	TCATGA	3 cut(s)	1575, 1732, 2518
Cfr10I	RCCGGY	2 cut(s)	2766, 2872
Cfr13I	GGNCC	3 cut(s)	1541, 1583, 1592
Cfr9I	CCCGGG	1 cut(s)	2680
CseI	GACGC	2 cut(s)	1139, 1657
Csp6I	GTAC	3 cut(s)	160, 2104, 2194
CviQI	GTAC	3 cut(s)	160, 2104, 2194
DrdI	GACNNNNNNGTC	1 cut(s)	16
DseDI	GACNNNNNNGTC	1 cut(s)	16
EaeI	YGGCCR	3 cut(s)	667, 1702, 2768
Eam1104I	CTCTTC	3 cut(s)	9, 192, 2594
EarI	CTCTTC	3 cut(s)	9, 192, 2594
Eco105I	TACGTA	1 cut(s)	2857
Eco130I	CCWWGG	4 cut(s)	585, 924, 1486, 1537
Eco31I	GGTCTC	1 cut(s)	1161
Eco47I	GGWCC	2 cut(s)	1541, 1583
Eco57I	CTGAAG	6 cut(s)	241, 1655, 2075, 2213, 2461, 2796
Eco81I	CCTNAGG	1 cut(s)	906
Eco88I	CYCGRG	3 cut(s)	1154, 2680, 2713
EcoO109I	RGGNCCY	1 cut(s)	1583
EcoRI	GAATTC	3 cut(s)	695, 1619, 1665
EcoRII	CCWGG	2 cut(s)	2560, 2661
EcoT14I	CCWWGG	4 cut(s)	585, 924, 1486, 1537
ErhI	CCWWGG	4 cut(s)	585, 924, 1486, 1537
Esp3I	CGTCTC	1 cut(s)	1157
FalI	AAGNNNNNCTT	2 cut(s)	740, 772
FaqI	GGGAC	2 cut(s)	502, 564
FbaI	TGATCA	3 cut(s)	603, 1504, 2703
FokI	GGATG	9 cut(s)	722, 893, 941, 1349, 1915, 2190, 2259, 2441, 2807
FspBI	CTAG	6 cut(s)	576, 651, 1079, 1638, 1880, 2909
GsuI	CTGGAG	2 cut(s)	1988, 2971
HaeIII	GGCC	7 cut(s)	466, 669, 1476, 1594, 1704, 2335, 2770
HapII	CCGG	6 cut(s)	656, 2221, 2681, 2767, 2873, 2891
HgaI	GACGC	2 cut(s)	1139, 1657
HindIII	AAGCTT	5 cut(s)	360, 710, 1493, 1688, 3044
HinfI	GANTC	9 cut(s)	17, 490, 676, 946, 1315, 1572, 1747, 1961, 2948
HpaII	CCGG	6 cut(s)	656, 2221, 2681, 2767, 2873, 2891
Hpy166II	GTNNAC	7 cut(s)	1433, 1874, 2129, 2695, 2808, 2836, 2865
Hpy8I	GTNNAC	7 cut(s)	1433, 1874, 2129, 2695, 2808, 2836, 2865
HpyCH4III	ACNGT	6 cut(s)	1270, 1395, 2133, 2185, 2689, 2701
HpyCH4IV	ACGT	1 cut(s)	2856
HpyF10VI	GCNNNNNNNGC	9 cut(s)	656, 757, 873, 1414, 1681, 1701, 2147, 2900, 2909
HpySE526I	ACGT	1 cut(s)	2856
KroI	GCCGGC	1 cut(s)	2766
KroNI	GCCGGC	1 cut(s)	2768
Ksp22I	TGATCA	3 cut(s)	603, 1504, 2703
LguI	GCTCTTC	1 cut(s)	192
LmnI	GCTCC	4 cut(s)	541, 1025, 1453, 2981
LweI	GCATC	5 cut(s)	834, 1893, 2000, 2168, 2614
MaeI	CTAG	6 cut(s)	576, 651, 1079, 1638, 1880, 2909
MaeII	ACGT	1 cut(s)	2856
MaeIII	GTNAC	8 cut(s)	26, 451, 776, 1102, 1708, 2266, 2367, 2564
MfeI	CAATTG	1 cut(s)	1905
MflI	RGATCY	1 cut(s)	2398
MhlI	GDGCHC	3 cut(s)	339, 2867, 2889
MlsI	TGGCCA	1 cut(s)	1704
MluNI	TGGCCA	1 cut(s)	1704
MlyI	GAGTC	3 cut(s)	26, 484, 1581
MmeI	TCCRAC	3 cut(s)	724, 2152, 2406
Mox20I	TGGCCA	1 cut(s)	1704
MroNI	GCCGGC	1 cut(s)	2766
MroXI	GAANNNNTTC	3 cut(s)	1269, 2657, 2673
MscI	TGGCCA	1 cut(s)	1704
MslI	CAYNNNNRTG	4 cut(s)	558, 1127, 2252, 2748
Msp20I	TGGCCA	1 cut(s)	1704
MspA1I	CMGCKG	1 cut(s)	2008
MspCI	CTTAAG	1 cut(s)	1523
MspI	CCGG	6 cut(s)	656, 2221, 2681, 2767, 2873, 2891
MspR9I	CCNGG	6 cut(s)	2221, 2562, 2663, 2681, 2682, 2891
MunI	CAATTG	1 cut(s)	1905
Mva1269I	GAATGC	2 cut(s)	1605, 1836
MvaI	CCWGG	2 cut(s)	2562, 2663
MvnI	CGCG	1 cut(s)	1651
MwoI	GCNNNNNNNGC	9 cut(s)	656, 757, 873, 1414, 1681, 1701, 2147, 2900, 2909
NaeI	GCCGGC	1 cut(s)	2768
NciI	CCSGG	4 cut(s)	2221, 2681, 2682, 2891
NgoMIV	GCCGGC	1 cut(s)	2766
NlaIV	GGNNCC	3 cut(s)	1816, 2886, 2977
NmuCI	GTSAC	4 cut(s)	451, 776, 1102, 2367
NspV	TTCGAA	2 cut(s)	839, 1923
PaeR7I	CTCGAG	1 cut(s)	1154
PagI	TCATGA	3 cut(s)	1575, 1732, 2518
PciSI	GCTCTTC	1 cut(s)	192
PctI	GAATGC	2 cut(s)	1605, 1836
PdiI	GCCGGC	1 cut(s)	2768
PdmI	GAANNNNTTC	3 cut(s)	1269, 2657, 2673
PfeI	GAWTC	6 cut(s)	676, 946, 1315, 1747, 1961, 2948
PflMI	CCANNNNNTGG	3 cut(s)	473, 1601, 2662
PfoI	TCCNGGA	1 cut(s)	2661
Ple19I	CGATCG	1 cut(s)	2945
PleI	GAGTC	3 cut(s)	25, 484, 1580
PpsI	GAGTC	3 cut(s)	25, 484, 1580
Ppu21I	YACGTR	1 cut(s)	2857
PpuMI	RGGWCCY	1 cut(s)	1583
Psp5II	RGGWCCY	1 cut(s)	1583
Psp6I	CCWGG	2 cut(s)	2560, 2661
PspGI	CCWGG	2 cut(s)	2560, 2661
PspN4I	GGNNCC	3 cut(s)	1816, 2886, 2977
PspPI	GGNCC	3 cut(s)	1541, 1583, 1592
PspPPI	RGGWCCY	1 cut(s)	1583
PstI	CTGCAG	2 cut(s)	2341, 2826
PstNI	CAGNNNCTG	2 cut(s)	2662, 2830
PsuI	RGATCY	1 cut(s)	2398
PvuI	CGATCG	1 cut(s)	2945
PvuII	CAGCTG	1 cut(s)	2008
RsaI	GTAC	3 cut(s)	161, 2105, 2195
RsaNI	GTAC	3 cut(s)	160, 2104, 2194
RseI	CAYNNNNRTG	4 cut(s)	558, 1127, 2252, 2748
SapI	GCTCTTC	1 cut(s)	192
Sau96I	GGNCC	3 cut(s)	1541, 1583, 1592
ScaI	AGTACT	1 cut(s)	161
SchI	GAGTC	3 cut(s)	26, 484, 1581
ScrFI	CCNGG	6 cut(s)	2221, 2562, 2663, 2681, 2682, 2891
SduI	GDGCHC	3 cut(s)	339, 2867, 2889
SfaNI	GCATC	5 cut(s)	834, 1893, 2000, 2168, 2614
SfcI	CTRYAG	3 cut(s)	1029, 2337, 2822
Sfr274I	CTCGAG	1 cut(s)	1154
SfuI	TTCGAA	2 cut(s)	839, 1923
SinI	GGWCC	2 cut(s)	1541, 1583
SlaI	CTCGAG	1 cut(s)	1154
SmaI	CCCGGG	1 cut(s)	2682
SmiMI	CAYNNNNRTG	4 cut(s)	558, 1127, 2252, 2748
SmlI	CTYRAG	4 cut(s)	1154, 1523, 2153, 2936
SmoI	CTYRAG	4 cut(s)	1154, 1523, 2153, 2936
SnaBI	TACGTA	1 cut(s)	2857
SsiI	CCGC	3 cut(s)	670, 1107, 2066
SspMI	CTAG	6 cut(s)	576, 651, 1079, 1638, 1880, 2909
StyD4I	CCNGG	6 cut(s)	2219, 2560, 2661, 2679, 2680, 2889
StyI	CCWWGG	4 cut(s)	585, 924, 1486, 1537
TaaI	ACNGT	6 cut(s)	1270, 1395, 2133, 2185, 2689, 2701
TaiI	ACGT	1 cut(s)	2859
TaqI	TCGA	8 cut(s)	347, 598, 693, 839, 1155, 1165, 1923, 2286
TaqII	GACCGA	1 cut(s)	1558
TatI	WGTACW	3 cut(s)	159, 2103, 2193
TauI	GCSGC	1 cut(s)	672
TfiI	GAWTC	6 cut(s)	676, 946, 1315, 1747, 1961, 2948
TscAI	CASTG	4 cut(s)	1315, 1748, 1838, 2595
TseFI	GTSAC	4 cut(s)	451, 776, 1102, 2367
Tsp45I	GTSAC	4 cut(s)	451, 776, 1102, 2367
TspGWI	ACGGA	3 cut(s)	1090, 1619, 2628
TspMI	CCCGGG	1 cut(s)	2680
TspRI	CASTG	4 cut(s)	1315, 1748, 1838, 2595
Van91I	CCANNNNNTGG	3 cut(s)	473, 1601, 2662
Vha464I	CTTAAG	1 cut(s)	1523
VneI	GTGCAC	1 cut(s)	2863
VpaK11BI	GGWCC	2 cut(s)	1541, 1583
XbaI	TCTAGA	1 cut(s)	1637
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1121, 1484
XhoI	CTCGAG	1 cut(s)	1154
XmaI	CCCGGG	1 cut(s)	2680
XmnI	GAANNNNTTC	3 cut(s)	1269, 2657, 2673
XspI	CTAG	6 cut(s)	576, 651, 1079, 1638, 1880, 2909
ZrmI	AGTACT	1 cut(s)	161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.