Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0014403

Physical Location & Seq

Genomic Coordinates Reverse (-)

59086152 .. 59090095

Gene Structure 3944 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG372800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3054 bp

ATGGAAGAGACAGAAGAGTCCAATGGCAACAAGAAATGCAACACCAAAATCATAAGGGGCAGAACCAACAACCCACCTCAGAAGCAGCATCACCATCACCATCACCAGCTAATACAATACTCAAATCAGTTTGGGTTCTTCAACCAGAACCAGTACTATCAGAGATACTACTATCCAGCTCTTCTTCCCCTCCCTCCTCTTATACCTCTGCCTCTGGCTTTAACTCCACCCTTGCCTCAGAACCACAGCTTCAGAACAAAAGCCCATTTGCAGAAACCTTCATGCAAGCACAAGAACTCTCCTTTTGCTGCTTCCTCAGAAACCCAAGTGCCCAAAGTCTCGATTTCACCAGCTTCAAAAGGGTTTCAAAAGCAAACCAATTTGCCCCTTAAAGGAGAAAATGGAAGGAAATTCACAAGTGCAAGAACAGGGAAAGCAATAGTGACAGCAAAGAGGCCAGACTCTGGTGGGGTGGAAGGGACTGCTATTTCTCTCTTAGCTAACCATTTCCTTGTCCAATTCAATTCATCGGAGCGAATTTTTCACTACAATGTTGAAATCTCTCCTAGTCCCTCCAAGGACATTTCTCGAATGATCAAGCAAAAACTGGTGGAGGATAACTCAGCTTTGCTCTGTGGTGCTAGTCCGGCTTATGATGGCCGCAAGAATCTTTACAGCTCAATCGAATTCCAACATGATAAGCTTGAAATGTATGTAAGCCTCCCCATCCCTACAACCAAGCCAACCTTGCCATGTGGGGAATTTGGTGACTTTCAAGAGAAGGATCATCAAATACTTAAACTTTTTCGTGTAAGCATCAAACTGGTTTCGAAGTTTGATGGAAAGGAATTGAGTAGCTACTTGAGCAAGGATGGAGATGAGTGGAAACCTATCCCTCAGGATTATCTTCATGCCTTGGATGTCGTTTTGAGAGAGAGTCCATTGGAGAAGTGTGTGCCTGTGGGAAGATCACTTTATTCCAGTTCAATGGGAGGAACTAAAGACATTGGAGGAGGAGCTGTAGGATATAGAGGGTTCTTTCAGAGCCTTAGACCAACCCAACAAGGACTAGCTCTCAATGTTGATTTCTCCGTGACCGCTTTCCATGAAAGCATTGGAATGATCCCATACTTGCAGAAGCGTCTCGAGTTTCTTCGAGACCTTCCTCAGAGGAAGACAAGGGGTTTAAGTAGTGAAGAAAGGAAAGAAGTGGAGAAGGCATTGAAGAACATCAGGGTCTTTGTTTGCCATAGAGAAACAGTTCAGAGATACCGAGTTTTTGGCTTAACCGAGGAAGCCACTGAGAATCTTTGGTTTCCAGACAGGGATGGGAAGAATTTGAGGCTGCTGACTTACTTCAAGGATCACTACAACTATGATATACAGTTCAGGAATTTGCCATGTTTGCAGATTAGTAGGAGTAAACCATGTTATCTTCCTATGGAGCTATGCATGATCTGTGAAGGCCAGAAGTTCCTTGGGAAGCTTTCAGATGATCAGACTGCAAGGATACTTAAGATGGGCTGCCAAGGACCGAAAGAACGAAAATCCATCATTGATGGAGTCATGAGAGGACCTGTTGGGCCAATAAGTGGCATTCAGGAAAGGGAATTCAAACTCCGTGTTTCTAGAGAAATGACGCGATTAAATGGAAGAATTCTGCAGCCTCCAAAGCTAAAGCTTGGCGATGGTGGCCATGTAACAGACCTCATTCCTTCCCGTCATGATCGGCAGTGGAATCTTCTGGACAGCCATGTGTTTGAAGGAACTTGCGTTGAGAGGTGGGCATTGATTAGTTTTGGTGGCACCCCAGAACAGAAGCACTGCATTCCCAAATTCATACACCAGCTATCTCAAAGATGTGAACAACTAGGCATCTTTCTTAACAAAAACACAATTGTTAGCCCCCAATTCGAACCATCCCAAGTGCTTAACAATGTATCTCTTTTAGAATCTAAGATTAAGAAAATCCAAAGAGCTGCATCAAACAATCTCCAGCTGCTTATCTGTGTAATGGAAAGAAAACACAAAGGGTATGCAGACCTGAAGCGAATTGCGGATACAGGCGTTGGTGTTCTAACACAATGCTGCTTGTACTCAAACCTTTGCAGATTGAGTTCACAGTTTTTGGCTAACTTGGCTCTCAAGATCAATGCCAAAGTTGGAGGATGCACAGTTTCTTTGTACAACTCTTTGCCATCTCAAATCCCCCGGCTGCTTCAGAGTGATGAGCCAGTCATCTTTATGGGTGCTGATGTTACTCATCCTCACCCACTCGATGATTTCAGCCCTTCTGTTGCTGCTGTGGTTGGTAGCATGAATTGGCCTGCAGCAAACAAGTATGTTTCAAGAATGAGGTCACAAACACATAGGCAAGAAATCATTGAGGATCTTGGTGAAATGGTGGAAGAATTGTTGGATGAGTTTCACCAAGAAGTAGGCAAACTCCCAAGGAGAATAGTTTTCTTCAGAGATGGGGTAAGCGAAACACAATTCTACAAAGTGCTTCATGAAGAATTGCAAGCCATTAAAAAAGGTTGTTCTAAATTCCCTGGTTACACACCTCCCATAACCTTTGCAGTGGTTCAGAAGAGGCATCACACAAGATTGTTCCCTTTCAAAATTGATCCGTCTTCCAAACAGAACCAGTTCCTCAACGAAAACATTCCCCCGGGAACTGTGGTGGACACGGTGATCACTCACCCGAGAGAGTTTGATTTCTATCTTTGTAGCCATTGGGGTGTTAAGGGAACAAGCCGGCCAACACATTACCACATTCTGTGGGATGAAAACGAGTTCACTTCAGATGAACTGCAGAAACTGGTGAACATTCTGTGCTACACATACGTAAGGTGCACCAAGCCGGTTTCTCTGGTGCCCCCGGCTTATTATGCTCACCTAGCTGCGTATAGAGGAAGGCTTTACCTTGAGCGATCGGAATCCACAACAGCTTACACAAGAAGTGGGTCCATCCTCTCCAGAGCTGCCCCTCTAAAGGCAATGGCTCTACCAAAACTTAGTGAGAATGTTAGGAAGCTTATGTTTTACTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009888 GO:0009892 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0019827 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035821 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043207 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0098542 GO:0098586 GO:0098727 GO:1901360 GO:1901698 GO:1901699

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

1017

Amino Acids

115.66

Weight (kDa)

9.28

Isoelectric Point (pI)

45.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	162 - 310	1.2e-27	N-terminal domain of argonaute
ArgoL1	PF08699	320 - 369	7.8e-17	Argonaute linker 1 domain
PAZ	PF02170	382 - 504	3.5e-28	PAZ domain
ArgoL2	PF16488	514 - 560	1.7e-06	Argonaute linker 2 domain
Piwi	PF02171	668 - 974	5.3e-91	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69440
fragaria_vesca	FvH4_4g30730
malus_domestica	MD13G1068200.v1.1 MD16G1067000.v1.1
prunus_persica	Prupe.1G279900_v2.0.a1
pyrus_communis	pycom13g06000 pycom16g05840
rosa_chinensis	RchiOBHm_Chr4g0439121
rosa_laevigata	RLG00000006296
rosa_roxburghii	Rroxscaffold_5G00380110
rosa_rugosa	Rorug04G0315900
rosa_samantha	Rh4AG366700 Rh4BG378200 Rh4CG392300 Rh4DG372800
rosa_wichuraiana	Rw0G007490 Rw0G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	16
AccB1I	GGYRCC	2 cut(s)	1805, 2875
AccB7I	CCANNNNNTGG	2 cut(s)	464, 1592
AccII	CGCG	1 cut(s)	1642
AciI	CCGC	3 cut(s)	661, 1098, 2057
AclWI	GGATC	5 cut(s)	792, 1117, 1371, 2397, 2621
AcoI	YGGCCR	3 cut(s)	658, 1693, 2759
AcuI	CTGAAG	5 cut(s)	235, 2066, 2204, 2452, 2787
AfaI	GTAC	3 cut(s)	155, 2096, 2186
AfiI	CCNNNNNNNGG	4 cut(s)	392, 464, 1592, 2995
AflII	CTTAAG	1 cut(s)	1514
AjnI	CCWGG	1 cut(s)	2551
Alw21I	GWGCWC	1 cut(s)	2858
Alw26I	GTCTC	4 cut(s)	2, 343, 1148, 1152
Alw44I	GTGCAC	1 cut(s)	2854
AlwI	GGATC	5 cut(s)	792, 1117, 1371, 2397, 2621
AlwNI	CAGNNNCTG	2 cut(s)	464, 2821
Ama87I	CYCGRG	3 cut(s)	1145, 2671, 2704
AoxI	GGCC	7 cut(s)	455, 658, 1465, 1583, 1693, 2324, 2759
ApaLI	GTGCAC	1 cut(s)	2854
Asp700I	GAANNNNTTC	3 cut(s)	1260, 2648, 2664
AspS9I	GGNCC	4 cut(s)	1532, 1574, 1583, 2967
AsuC2I	CCSGG	4 cut(s)	2212, 2672, 2673, 2882
AsuII	TTCGAA	2 cut(s)	830, 1914
AvaI	CYCGRG	3 cut(s)	1145, 2671, 2704
AvaII	GGWCC	3 cut(s)	1532, 1574, 2967
AxyI	CCTNAGG	1 cut(s)	897
BaeGI	GKGCMC	3 cut(s)	333, 2858, 2880
BaeI	ACNNNNGTAYC	2 cut(s)	2052, 2085
BalI	TGGCCA	1 cut(s)	1695
BanI	GGYRCC	2 cut(s)	1805, 2875
BbsI	GAAGAC	2 cut(s)	1181, 2625
Bbv12I	GWGCWC	1 cut(s)	2858
BciT130I	CCWGG	1 cut(s)	2553
BciVI	GTATCC	2 cut(s)	1503, 2053
BclI	TGATCA	3 cut(s)	594, 1495, 2694
BcnI	CCSGG	4 cut(s)	2212, 2672, 2673, 2882
BcoDI	GTCTC	4 cut(s)	2, 343, 1148, 1152
BfaI	CTAG	6 cut(s)	567, 642, 1070, 1629, 1871, 2900
BfmI	CTRYAG	4 cut(s)	1020, 1661, 2328, 2813
BfrI	CTTAAG	1 cut(s)	1514
BfuI	GTATCC	2 cut(s)	1503, 2053
BmcAI	AGTACT	1 cut(s)	155
Bme1390I	CCNGG	5 cut(s)	2212, 2553, 2672, 2673, 2882
Bme18I	GGWCC	3 cut(s)	1532, 1574, 2967
BmeT110I	CYCGRG	3 cut(s)	1145, 2671, 2704
BmgT120I	GGNCC	4 cut(s)	1532, 1574, 1583, 2967
BmiI	GGNNCC	3 cut(s)	1807, 2877, 2968
BmrFI	CCNGG	5 cut(s)	2212, 2553, 2672, 2673, 2882
BmsI	GCATC	6 cut(s)	97, 825, 1884, 1991, 2159, 2605
BpiI	GAAGAC	2 cut(s)	1181, 2625
BplI	GAGNNNNNCTC	2 cut(s)	605, 637
BpmI	CTGGAG	2 cut(s)	1979, 2962
Bpu14I	TTCGAA	2 cut(s)	830, 1914
BpuEI	CTTGAG	3 cut(s)	883, 2129, 2948
BpuMI	CCSGG	4 cut(s)	2212, 2672, 2673, 2882
BsaAI	YACGTR	1 cut(s)	2848
BsaBI	GATNNNNATC	2 cut(s)	2721, 2938
BsaI	GGTCTC	1 cut(s)	1152
BsaXI	ACNNNNNCTCC	2 cut(s)	1005, 1035
Bsc4I	CCNNNNNNNGG	4 cut(s)	392, 464, 1592, 2995
Bse118I	RCCGGY	2 cut(s)	2757, 2863
Bse1I	ACTGG	7 cut(s)	151, 612, 828, 981, 2234, 2647, 2826
Bse21I	CCTNAGG	1 cut(s)	897
Bse3DI	GCAATG	1 cut(s)	3006
Bse8I	GATNNNNATC	2 cut(s)	2721, 2938
BseBI	CCWGG	1 cut(s)	2553
BseJI	GATNNNNATC	2 cut(s)	2721, 2938
BseLI	CCNNNNNNNGG	4 cut(s)	392, 464, 1592, 2995
BseMI	GCAATG	1 cut(s)	3006
BseMII	CTCAG	7 cut(s)	92, 251, 330, 636, 911, 1181, 1293
BseNI	ACTGG	7 cut(s)	151, 612, 828, 981, 2234, 2647, 2826
BseRI	GAGGAG	3 cut(s)	186, 1026, 1029
BseSI	GKGCMC	3 cut(s)	333, 2858, 2880
Bsh1236I	CGCG	1 cut(s)	1642
Bsh1285I	CGRYCG	1 cut(s)	2936
BshFI	GGCC	7 cut(s)	457, 660, 1467, 1585, 1695, 2326, 2761
BshNI	GGYRCC	2 cut(s)	1805, 2875
BsiEI	CGRYCG	1 cut(s)	2936
BsiHKAI	GWGCWC	1 cut(s)	2858
BsiHKCI	CYCGRG	3 cut(s)	1145, 2671, 2704
BsiSI	CCGG	6 cut(s)	647, 2212, 2672, 2758, 2864, 2882
BslFI	GGGAC	2 cut(s)	493, 555
BslI	CCNNNNNNNGG	4 cut(s)	392, 464, 1592, 2995
BsmAI	GTCTC	4 cut(s)	2, 343, 1148, 1152
BsmBI	CGTCTC	1 cut(s)	1148
BsmFI	GGGAC	2 cut(s)	493, 555
BsmI	GAATGC	2 cut(s)	1596, 1827
BsnI	GGCC	7 cut(s)	457, 660, 1467, 1585, 1695, 2326, 2761
Bso31I	GGTCTC	1 cut(s)	1152
BsoBI	CYCGRG	3 cut(s)	1145, 2671, 2704
Bsp119I	TTCGAA	2 cut(s)	830, 1914
Bsp1286I	GDGCHC	3 cut(s)	333, 2858, 2880
Bsp1407I	TGTACA	1 cut(s)	2184
BspACI	CCGC	3 cut(s)	661, 1098, 2057
BspANI	GGCC	7 cut(s)	457, 660, 1467, 1585, 1695, 2326, 2761
BspCNI	CTCAG	7 cut(s)	91, 250, 329, 635, 910, 1180, 1294
BspFNI	CGCG	1 cut(s)	1642
BspHI	TCATGA	3 cut(s)	1566, 1723, 2509
BspLI	GGNNCC	3 cut(s)	1807, 2877, 2968
BspMAI	CTGCAG	3 cut(s)	1665, 2332, 2817
BspPI	GGATC	5 cut(s)	792, 1117, 1371, 2397, 2621
BspQI	GCTCTTC	1 cut(s)	186
BspT104I	TTCGAA	2 cut(s)	830, 1914
BspT107I	GGYRCC	2 cut(s)	1805, 2875
BspTI	CTTAAG	1 cut(s)	1514
BspTNI	GGTCTC	1 cut(s)	1152
BsrDI	GCAATG	1 cut(s)	3006
BsrFI	RCCGGY	2 cut(s)	2757, 2863
BsrGI	TGTACA	1 cut(s)	2184
BsrI	ACTGG	7 cut(s)	151, 612, 828, 981, 2234, 2647, 2826
BssAI	RCCGGY	2 cut(s)	2757, 2863
BssT1I	CCWWGG	5 cut(s)	576, 915, 1477, 1528, 2450
Bst2UI	CCWGG	1 cut(s)	2553
Bst4CI	ACNGT	6 cut(s)	1261, 1386, 2124, 2176, 2680, 2692
Bst6I	CTCTTC	3 cut(s)	9, 186, 2585
BstAFI	CTTAAG	1 cut(s)	1514
BstAUI	TGTACA	1 cut(s)	2184
BstBAI	YACGTR	1 cut(s)	2848
BstBI	TTCGAA	2 cut(s)	830, 1914
BstC8I	GCNNGC	4 cut(s)	287, 2328, 2523, 2759
BstFNI	CGCG	1 cut(s)	1642
BstMAI	GTCTC	4 cut(s)	2, 343, 1148, 1152
BstMCI	CGRYCG	1 cut(s)	2936
BstMWI	GCNNNNNNNGC	9 cut(s)	647, 748, 864, 1405, 1672, 1692, 2138, 2891, 2900
BstNI	CCWGG	1 cut(s)	2553
BstSCI	CCNGG	5 cut(s)	2210, 2551, 2670, 2671, 2880
BstSFI	CTRYAG	4 cut(s)	1020, 1661, 2328, 2813
BstSLI	GKGCMC	3 cut(s)	333, 2858, 2880
BstSNI	TACGTA	1 cut(s)	2848
BstUI	CGCG	1 cut(s)	1642
BstV2I	GAAGAC	2 cut(s)	1181, 2625
BstX2I	RGATCY	1 cut(s)	2389
BstXI	CCANNNNNNTGG	1 cut(s)	988
BstYI	RGATCY	1 cut(s)	2389
Bsu36I	CCTNAGG	1 cut(s)	897
BsuI	GTATCC	2 cut(s)	1503, 2053
BsuRI	GGCC	7 cut(s)	457, 660, 1467, 1585, 1695, 2326, 2761
BtgZI	GCGATG	1 cut(s)	1701
BtsI	GCAGTG	3 cut(s)	1739, 1822, 2586
BtsIMutI	CAGTG	4 cut(s)	1299, 1739, 1822, 2586
Cac8I	GCNNGC	4 cut(s)	287, 2328, 2523, 2759
CaiI	CAGNNNCTG	2 cut(s)	464, 2821
CciI	TCATGA	3 cut(s)	1566, 1723, 2509
Cfr10I	RCCGGY	2 cut(s)	2757, 2863
Cfr13I	GGNCC	4 cut(s)	1532, 1574, 1583, 2967
Cfr9I	CCCGGG	1 cut(s)	2671
CseI	GACGC	2 cut(s)	1130, 1648
Csp6I	GTAC	3 cut(s)	154, 2095, 2185
CviQI	GTAC	3 cut(s)	154, 2095, 2185
DrdI	GACNNNNNNGTC	1 cut(s)	16
DseDI	GACNNNNNNGTC	1 cut(s)	16
EaeI	YGGCCR	3 cut(s)	658, 1693, 2759
Eam1104I	CTCTTC	3 cut(s)	9, 186, 2585
EarI	CTCTTC	3 cut(s)	9, 186, 2585
Eco105I	TACGTA	1 cut(s)	2848
Eco130I	CCWWGG	5 cut(s)	576, 915, 1477, 1528, 2450
Eco31I	GGTCTC	1 cut(s)	1152
Eco47I	GGWCC	3 cut(s)	1532, 1574, 2967
Eco57I	CTGAAG	5 cut(s)	235, 2066, 2204, 2452, 2787
Eco81I	CCTNAGG	1 cut(s)	897
Eco88I	CYCGRG	3 cut(s)	1145, 2671, 2704
EcoO109I	RGGNCCY	1 cut(s)	1574
EcoRI	GAATTC	3 cut(s)	686, 1610, 1656
EcoRII	CCWGG	1 cut(s)	2551
EcoT14I	CCWWGG	5 cut(s)	576, 915, 1477, 1528, 2450
EcoT22I	ATGCAT	1 cut(s)	1454
ErhI	CCWWGG	5 cut(s)	576, 915, 1477, 1528, 2450
Esp3I	CGTCTC	1 cut(s)	1148
FalI	AAGNNNNNCTT	2 cut(s)	731, 763
FaqI	GGGAC	2 cut(s)	493, 555
FbaI	TGATCA	3 cut(s)	594, 1495, 2694
FspBI	CTAG	6 cut(s)	567, 642, 1070, 1629, 1871, 2900
GsuI	CTGGAG	2 cut(s)	1979, 2962
HaeIII	GGCC	7 cut(s)	457, 660, 1467, 1585, 1695, 2326, 2761
HapII	CCGG	6 cut(s)	647, 2212, 2672, 2758, 2864, 2882
HgaI	GACGC	2 cut(s)	1130, 1648
HindIII	AAGCTT	4 cut(s)	701, 1484, 1679, 3035
HinfI	GANTC	9 cut(s)	17, 461, 667, 937, 1306, 1563, 1738, 1952, 2939
HpaII	CCGG	6 cut(s)	647, 2212, 2672, 2758, 2864, 2882
Hpy166II	GTNNAC	7 cut(s)	1424, 1865, 2120, 2686, 2799, 2827, 2856
Hpy8I	GTNNAC	7 cut(s)	1424, 1865, 2120, 2686, 2799, 2827, 2856
HpyCH4III	ACNGT	6 cut(s)	1261, 1386, 2124, 2176, 2680, 2692
HpyCH4IV	ACGT	1 cut(s)	2847
HpyF10VI	GCNNNNNNNGC	9 cut(s)	647, 748, 864, 1405, 1672, 1692, 2138, 2891, 2900
HpySE526I	ACGT	1 cut(s)	2847
KroI	GCCGGC	1 cut(s)	2757
KroNI	GCCGGC	1 cut(s)	2759
Ksp22I	TGATCA	3 cut(s)	594, 1495, 2694
LguI	GCTCTTC	1 cut(s)	186
LmnI	GCTCC	3 cut(s)	532, 1016, 1444
LweI	GCATC	6 cut(s)	97, 825, 1884, 1991, 2159, 2605
MaeI	CTAG	6 cut(s)	567, 642, 1070, 1629, 1871, 2900
MaeII	ACGT	1 cut(s)	2847
MaeIII	GTNAC	7 cut(s)	442, 767, 1093, 1699, 2257, 2358, 2555
MfeI	CAATTG	1 cut(s)	1896
MflI	RGATCY	1 cut(s)	2389
MhlI	GDGCHC	3 cut(s)	333, 2858, 2880
MlsI	TGGCCA	1 cut(s)	1695
MluNI	TGGCCA	1 cut(s)	1695
MlyI	GAGTC	4 cut(s)	26, 455, 946, 1572
MmeI	TCCRAC	3 cut(s)	715, 2143, 2397
Mox20I	TGGCCA	1 cut(s)	1695
Mph1103I	ATGCAT	1 cut(s)	1454
MroNI	GCCGGC	1 cut(s)	2757
MroXI	GAANNNNTTC	3 cut(s)	1260, 2648, 2664
MscI	TGGCCA	1 cut(s)	1695
MslI	CAYNNNNRTG	4 cut(s)	549, 1118, 2243, 2739
Msp20I	TGGCCA	1 cut(s)	1695
MspA1I	CMGCKG	1 cut(s)	1999
MspCI	CTTAAG	1 cut(s)	1514
MspI	CCGG	6 cut(s)	647, 2212, 2672, 2758, 2864, 2882
MspR9I	CCNGG	5 cut(s)	2212, 2553, 2672, 2673, 2882
MunI	CAATTG	1 cut(s)	1896
Mva1269I	GAATGC	2 cut(s)	1596, 1827
MvaI	CCWGG	1 cut(s)	2553
MvnI	CGCG	1 cut(s)	1642
MwoI	GCNNNNNNNGC	9 cut(s)	647, 748, 864, 1405, 1672, 1692, 2138, 2891, 2900
NaeI	GCCGGC	1 cut(s)	2759
NciI	CCSGG	4 cut(s)	2212, 2672, 2673, 2882
NgoMIV	GCCGGC	1 cut(s)	2757
NlaIV	GGNNCC	3 cut(s)	1807, 2877, 2968
NmuCI	GTSAC	4 cut(s)	442, 767, 1093, 2358
NsiI	ATGCAT	1 cut(s)	1454
NspV	TTCGAA	2 cut(s)	830, 1914
PaeR7I	CTCGAG	1 cut(s)	1145
PagI	TCATGA	3 cut(s)	1566, 1723, 2509
PciSI	GCTCTTC	1 cut(s)	186
PctI	GAATGC	2 cut(s)	1596, 1827
PdiI	GCCGGC	1 cut(s)	2759
PdmI	GAANNNNTTC	3 cut(s)	1260, 2648, 2664
PfeI	GAWTC	5 cut(s)	667, 1306, 1738, 1952, 2939
PflMI	CCANNNNNTGG	2 cut(s)	464, 1592
Ple19I	CGATCG	1 cut(s)	2936
PleI	GAGTC	4 cut(s)	25, 455, 945, 1571
PpsI	GAGTC	4 cut(s)	25, 455, 945, 1571
Ppu21I	YACGTR	1 cut(s)	2848
PpuMI	RGGWCCY	1 cut(s)	1574
Psp5II	RGGWCCY	1 cut(s)	1574
Psp6I	CCWGG	1 cut(s)	2551
PspGI	CCWGG	1 cut(s)	2551
PspN4I	GGNNCC	3 cut(s)	1807, 2877, 2968
PspPI	GGNCC	4 cut(s)	1532, 1574, 1583, 2967
PspPPI	RGGWCCY	1 cut(s)	1574
PstI	CTGCAG	3 cut(s)	1665, 2332, 2817
PstNI	CAGNNNCTG	2 cut(s)	464, 2821
PsuI	RGATCY	1 cut(s)	2389
PvuI	CGATCG	1 cut(s)	2936
PvuII	CAGCTG	1 cut(s)	1999
RsaI	GTAC	3 cut(s)	155, 2096, 2186
RsaNI	GTAC	3 cut(s)	154, 2095, 2185
RseI	CAYNNNNRTG	4 cut(s)	549, 1118, 2243, 2739
SapI	GCTCTTC	1 cut(s)	186
Sau96I	GGNCC	4 cut(s)	1532, 1574, 1583, 2967
ScaI	AGTACT	1 cut(s)	155
SchI	GAGTC	4 cut(s)	26, 455, 946, 1572
ScrFI	CCNGG	5 cut(s)	2212, 2553, 2672, 2673, 2882
SduI	GDGCHC	3 cut(s)	333, 2858, 2880
SfaNI	GCATC	6 cut(s)	97, 825, 1884, 1991, 2159, 2605
SfcI	CTRYAG	4 cut(s)	1020, 1661, 2328, 2813
Sfr274I	CTCGAG	1 cut(s)	1145
SfuI	TTCGAA	2 cut(s)	830, 1914
SinI	GGWCC	3 cut(s)	1532, 1574, 2967
SlaI	CTCGAG	1 cut(s)	1145
SmaI	CCCGGG	1 cut(s)	2673
SmiMI	CAYNNNNRTG	4 cut(s)	549, 1118, 2243, 2739
SmlI	CTYRAG	5 cut(s)	862, 1145, 1514, 2144, 2927
SmoI	CTYRAG	5 cut(s)	862, 1145, 1514, 2144, 2927
SnaBI	TACGTA	1 cut(s)	2848
SsiI	CCGC	3 cut(s)	661, 1098, 2057
SspMI	CTAG	6 cut(s)	567, 642, 1070, 1629, 1871, 2900
StyD4I	CCNGG	5 cut(s)	2210, 2551, 2670, 2671, 2880
StyI	CCWWGG	5 cut(s)	576, 915, 1477, 1528, 2450
TaaI	ACNGT	6 cut(s)	1261, 1386, 2124, 2176, 2680, 2692
TaiI	ACGT	1 cut(s)	2850
TaqI	TCGA	8 cut(s)	341, 589, 684, 830, 1146, 1156, 1914, 2277
TaqII	GACCGA	1 cut(s)	1549
TatI	WGTACW	3 cut(s)	153, 2094, 2184
TauI	GCSGC	1 cut(s)	663
TfiI	GAWTC	5 cut(s)	667, 1306, 1738, 1952, 2939
TscAI	CASTG	4 cut(s)	1306, 1739, 1829, 2586
TseFI	GTSAC	4 cut(s)	442, 767, 1093, 2358
Tsp45I	GTSAC	4 cut(s)	442, 767, 1093, 2358
TspGWI	ACGGA	3 cut(s)	1081, 1610, 2619
TspMI	CCCGGG	1 cut(s)	2671
TspRI	CASTG	4 cut(s)	1306, 1739, 1829, 2586
Van91I	CCANNNNNTGG	2 cut(s)	464, 1592
Vha464I	CTTAAG	1 cut(s)	1514
VneI	GTGCAC	1 cut(s)	2854
VpaK11BI	GGWCC	3 cut(s)	1532, 1574, 2967
XbaI	TCTAGA	1 cut(s)	1628
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1112, 1475
XhoI	CTCGAG	1 cut(s)	1145
XmaI	CCCGGG	1 cut(s)	2671
XmnI	GAANNNNTTC	3 cut(s)	1260, 2648, 2664
XspI	CTAG	6 cut(s)	567, 642, 1070, 1629, 1871, 2900
ZrmI	AGTACT	1 cut(s)	155
Zsp2I	ATGCAT	1 cut(s)	1454

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.