MD13G1074000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015491
Physical Location & Seq
Reverse (-)
5208302 .. 5208883
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1074000.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCTATTGCCGATCATACTCCGGCTAAACAGCAGAGGGATGAAACCCTAACCCCCGTTGACGACAGTGTTCGTCAAGCTACGTCTAAACGCCAAAGGGACGAAACACTAACCCTAGATGAGGATTCCGTTGATCTCAAGCGTCAGAAGCCCTACACCGACATACTCTCTGTCCTCGACGAAGAAGAAGTTGAAACTTCGGAGGACTTGTCCTCTCTAATCACATCCCTGCAGCAAGAAATCTCCTCTGAGCCTCTCAACACATTCCACGGCTCCGAAACCCTAGCTGCTGAGTCGGCTCTCGAAGGCTATGCCTCGTCCTCTGGCTCTTCCACTCCTACTTCTTCTAATGCATGCTTTTTGAAGGAGGATGAGAGCGAGAGGGTTTTGCGACGTCTTTTAGAAGCTTCCGATGACGAGCTCGGGATTCCGCAGAGGGAGGCTGACGGGTTCGATGACGAGGCGGGTAACGGGTTTAAATTCAATGGTCTGGATGGGTTTACTTTTGGTGATGGGTTGTGGGAGCTAGAGGACGAAGCTGCCAACTACTATACCTTGATGCAATCTGAACTGTTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

21.39

Weight (kDa)

4.07

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015491)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26920 AT1G69760
malus_domestica MD13G1074000.v1.1 MD16G1075200.v1.1
prunus_persica Prupe.1G272000_v2.0.a1
pyrus_communis pycom16g06530
rosa_chinensis RchiOBHm_Chr4g0440081
rosa_laevigata RLG00000006207
rosa_multiflora Rmu_sc0001685.1_g000006
rosa_roxburghii Rroxscaffold_5G00381020
rosa_rugosa Rorug04G0324200
rosa_samantha Rh4AG374900 Rh4BG387200 Rh4CG401700 Rh4DG381500
rosa_wichuraiana Rw4G032070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 397
AciI CCGC 2 cut(s) 431, 464
AcsI RAATTY 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 394
AfiI CCNNNNNNNGG 1 cut(s) 121
AgsI TTSAA 3 cut(s) 194, 364, 484
AluBI AGCT 6 cut(s) 80, 287, 407, 421, 526, 539
AluI AGCT 6 cut(s) 80, 287, 407, 421, 526, 539
Alw21I GWGCWC 1 cut(s) 423
Ama87I CYCGRG 1 cut(s) 422
ApeKI GCWGC 3 cut(s) 232, 287, 539
ApoI RAATTY 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 422
BanII GRGCYC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 244, 274, 526
BccI CCATC 2 cut(s) 488, 506
BceAI ACGGC 1 cut(s) 286
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BfaI CTAG 3 cut(s) 116, 284, 527
BfmI CTRYAG 1 cut(s) 230
BisI GCNGC 3 cut(s) 233, 288, 540
BlsI GCNGC 3 cut(s) 234, 289, 541
BmeT110I CYCGRG 1 cut(s) 422
BmiI GGNNCC 1 cut(s) 274
BmsI GCATC 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 394
BsaJI CCNNGG 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 268
BseGI GGATG 4 cut(s) 46, 224, 376, 499
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMII CTCAG 2 cut(s) 240, 282
BseRI GAGGAG 1 cut(s) 235
BseXI GCAGC 3 cut(s) 244, 274, 526
BsiHKAI GWGCWC 1 cut(s) 423
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 2 cut(s) 13, 133
BspACI CCGC 2 cut(s) 431, 464
BspCNI CTCAG 2 cut(s) 241, 283
BspLI GGNNCC 1 cut(s) 274
BspMAI CTGCAG 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 2 cut(s) 13, 133
BssNI GRCGYC 1 cut(s) 394
Bst4CI ACNGT 2 cut(s) 68, 573
Bst6I CTCTTC 1 cut(s) 334
BstACI GRCGYC 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 2 cut(s) 249, 291
BstDSI CCRYGG 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 4 cut(s) 46, 224, 376, 499
BstKTI GATC 2 cut(s) 16, 136
BstMBI GATC 2 cut(s) 13, 133
BstMWI GCNNNNNNNGC 2 cut(s) 148, 296
BstNSI RCATGY 1 cut(s) 357
BstSFI CTRYAG 1 cut(s) 230
BstV1I GCAGC 3 cut(s) 244, 274, 526
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 4 cut(s) 46, 224, 376, 499
BtsIMutI CAGTG 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 355
CseI GACGC 1 cut(s) 131
CviAII CATG 2 cut(s) 354, 577
DdeI CTNAG 2 cut(s) 249, 291
DpnI GATC 2 cut(s) 15, 135
DpnII GATC 2 cut(s) 13, 133
DraI TTTAAA 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 421
Eco24I GRGCYC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 421
Eco88I CYCGRG 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 421
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 423
FaeI CATG 2 cut(s) 357, 580
FaiI YATR 6 cut(s) 18, 164, 312, 355, 552, 578
FaqI GGGAC 1 cut(s) 113
FatI CATG 2 cut(s) 353, 576
FauI CCCGC 1 cut(s) 457
Fnu4HI GCNGC 3 cut(s) 233, 288, 540
FokI GGATG 4 cut(s) 53, 211, 383, 506
FriOI GRGCYC 1 cut(s) 423
Fsp4HI GCNGC 3 cut(s) 233, 288, 540
FspBI CTAG 3 cut(s) 116, 284, 527
GluI GCNGC 3 cut(s) 233, 288, 540
HapII CCGG 1 cut(s) 23
HgaI GACGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 394
Hin1II CATG 2 cut(s) 357, 580
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 3 cut(s) 125, 293, 427
HpaII CCGG 1 cut(s) 23
HphI GGTGA 1 cut(s) 521
Hpy166II GTNNAC 2 cut(s) 61, 501
Hpy188I TCNGA 6 cut(s) 147, 202, 250, 277, 412, 568
Hpy188III TCNNGA 3 cut(s) 302, 424, 491
Hpy8I GTNNAC 2 cut(s) 61, 501
Hpy99I CGWCG 2 cut(s) 182, 396
HpyAV CCTTC 2 cut(s) 299, 358
HpyCH4III ACNGT 2 cut(s) 68, 573
HpyCH4IV ACGT 2 cut(s) 83, 394
HpyCH4V TGCA 3 cut(s) 232, 353, 562
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 296
HpyF3I CTNAG 2 cut(s) 249, 291
HpySE526I ACGT 2 cut(s) 83, 394
Hsp92I GRCGYC 1 cut(s) 394
Hsp92II CATG 2 cut(s) 357, 580
Kzo9I GATC 2 cut(s) 13, 133
LguI GCTCTTC 1 cut(s) 334
LmnI GCTCC 2 cut(s) 278, 523
LpnPI CCDG 4 cut(s) 36, 242, 309, 476
Lsp1109I GCAGC 3 cut(s) 244, 274, 526
LweI GCATC 1 cut(s) 549
MaeI CTAG 3 cut(s) 116, 284, 527
MaeII ACGT 2 cut(s) 83, 394
MaeIII GTNAC 1 cut(s) 467
MalI GATC 2 cut(s) 15, 135
MboI GATC 2 cut(s) 13, 133
MboII GAAGA 4 cut(s) 194, 197, 321, 336
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 1 cut(s) 479
MlyI GAGTC 1 cut(s) 302
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 1 cut(s) 477
MspI CCGG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 148, 296
NdeII GATC 2 cut(s) 13, 133
NlaIII CATG 2 cut(s) 357, 580
NlaIV GGNNCC 1 cut(s) 274
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 1 cut(s) 357
PaeI GCATGC 1 cut(s) 357
PciSI GCTCTTC 1 cut(s) 334
PfeI GAWTC 2 cut(s) 125, 427
PkrI GCNGC 3 cut(s) 234, 289, 541
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
Psp124BI GAGCTC 1 cut(s) 423
PspN4I GGNNCC 1 cut(s) 274
PstI CTGCAG 1 cut(s) 234
SacI GAGCTC 1 cut(s) 423
SapI GCTCTTC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 3 cut(s) 233, 288, 540
Sau3AI GATC 2 cut(s) 13, 133
SchI GAGTC 1 cut(s) 302
SduI GDGCHC 1 cut(s) 423
SetI ASST 9 cut(s) 82, 86, 289, 397, 409, 423, 528, 541, 557
SfaNI GCATC 1 cut(s) 549
SfcI CTRYAG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 1 cut(s) 357
Sse9I AATT 1 cut(s) 479
SsiI CCGC 2 cut(s) 431, 464
SspMI CTAG 3 cut(s) 116, 284, 527
SstI GAGCTC 1 cut(s) 423
TaaI ACNGT 2 cut(s) 68, 573
TaiI ACGT 2 cut(s) 86, 397
TaqI TCGA 3 cut(s) 177, 303, 453
TasI AATT 1 cut(s) 479
TfiI GAWTC 2 cut(s) 125, 427
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 1 cut(s) 73
TseI GCWGC 3 cut(s) 232, 287, 539
TspDTI ATGAA 2 cut(s) 57, 565
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 1 cut(s) 73
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 1 cut(s) 479
XceI RCATGY 1 cut(s) 357
XspI CTAG 3 cut(s) 116, 284, 527
ZraI GACGTC 1 cut(s) 395
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.