Rw4G032070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015491
Physical Location & Seq
Reverse (-)
59197618 .. 59199565
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032070.1

Sequence Viewer

Length: 558 bp
ATGGCCATGGTTGAAACACAATCCTCAAAGCGCCAAAGAGATGACGAACAAGAAACCCAGATGTTAGAGGATCCTAAGCGCCAAAAGTCTTACAACCACATACTCTCTCTTCTTGATGAAGATGAAGAAGAACCCACCCAGGATTTGAACTCTCTCATCACAACCTTCCAGCAAGAGCTCTCCTCCGACTCCGACCTTGTTTTGAATCCGGACCACGTTGGTCACCAAACCATAGCAGATGCTTTGGATGCTTCCGAAGGGTACAACTCCTCTTCCTCCGGCTCTTCCTCACCTTCTTCTTCTTCGGCCCTGTTCTTGAAGGAGGGTGACGAGCAGGAGGACGAGAGTAGCGTCGAGAGGGTTATGAGACACCTTCTAGAAGCCTCCGACGACGAGCTAGGGATTCCGAATCGAGAGGCTGGTAATGGGTTTGAGGATGCGGCGGAGGCGGTGTTTAATTTTGGGGATAATGGGCTTTCACTTTGTGATGGGTTGTGGGAGCTTGAAGATGAAGCTGCCAATTACTATGCCCTGGTGCAGTCTGAACTGTTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.51

Weight (kDa)

4.05

Isoelectric Point (pI)

58.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015491)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26920 AT1G69760
malus_domestica MD13G1074000.v1.1 MD16G1075200.v1.1
prunus_persica Prupe.1G272000_v2.0.a1
pyrus_communis pycom16g06530
rosa_chinensis RchiOBHm_Chr4g0440081
rosa_laevigata RLG00000006207
rosa_multiflora Rmu_sc0001685.1_g000006
rosa_roxburghii Rroxscaffold_5G00381020
rosa_rugosa Rorug04G0324200
rosa_samantha Rh4AG374900 Rh4BG387200 Rh4CG401700 Rh4DG381500
rosa_wichuraiana Rw4G032070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 3 cut(s) 440, 443, 449
AclWI GGATC 2 cut(s) 65, 78
AcoI YGGCCR 1 cut(s) 3
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 1 cut(s) 263
AgsI TTSAA 5 cut(s) 14, 148, 205, 319, 506
AjnI CCWGG 2 cut(s) 138, 531
AluBI AGCT 4 cut(s) 178, 397, 502, 515
AluI AGCT 4 cut(s) 178, 397, 502, 515
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 2 cut(s) 65, 78
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 2 cut(s) 3, 306
ApeKI GCWGC 1 cut(s) 515
AspLEI GCGC 2 cut(s) 33, 81
AspS9I GGNCC 2 cut(s) 211, 307
AsuHPI GGTGA 3 cut(s) 215, 282, 338
AvaII GGWCC 1 cut(s) 211
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 502
BccI CCATC 1 cut(s) 482
BciT130I CCWGG 2 cut(s) 140, 533
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 2 cut(s) 377, 398
BfoI RGCGCY 2 cut(s) 34, 82
BisI GCNGC 2 cut(s) 441, 516
BlsI GCNGC 2 cut(s) 442, 517
Bme1390I CCNGG 2 cut(s) 140, 533
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 211, 307
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 140, 533
BmsI GCATC 3 cut(s) 229, 238, 427
BplI GAGNNNNNCTC 2 cut(s) 167, 199
Bpu10I CCTNAGC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 6, 138, 531
BsaWI WCCGGW 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 2 cut(s) 140, 533
BseDI CCNNGG 3 cut(s) 6, 138, 531
BseGI GGATG 2 cut(s) 253, 442
BseRI GAGGAG 2 cut(s) 172, 259
BseXI GCAGC 1 cut(s) 502
BsgI GTGCAG 1 cut(s) 557
BshFI GGCC 2 cut(s) 5, 308
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 2 cut(s) 209, 279
BsmAI GTCTC 1 cut(s) 361
BsnI GGCC 2 cut(s) 5, 308
Bsp1286I GDGCHC 1 cut(s) 180
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 1 cut(s) 70
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 3 cut(s) 440, 443, 449
BspANI GGCC 2 cut(s) 5, 308
BspEI TCCGGA 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 2 cut(s) 65, 78
BspQI GCTCTTC 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 6, 138, 531
BssMI GATC 1 cut(s) 70
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 140, 533
Bst4CI ACNGT 1 cut(s) 549
Bst6I CTCTTC 3 cut(s) 114, 277, 289
BstDEI CTNAG 1 cut(s) 75
BstDSI CCRYGG 1 cut(s) 6
BstEII GGTNACC 1 cut(s) 221
BstF5I GGATG 2 cut(s) 253, 442
BstH2I RGCGCY 2 cut(s) 34, 82
BstHHI GCGC 2 cut(s) 33, 81
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 1 cut(s) 70
BstMWI GCNNNNNNNGC 2 cut(s) 248, 446
BstNI CCWGG 2 cut(s) 140, 533
BstPI GGTNACC 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 138, 531
BstV1I GCAGC 1 cut(s) 502
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 2 cut(s) 5, 308
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 2 cut(s) 253, 442
CfoI GCGC 2 cut(s) 33, 81
Cfr13I GGNCC 2 cut(s) 211, 307
CseI GACGC 1 cut(s) 340
Csp6I GTAC 1 cut(s) 262
CviAII CATG 2 cut(s) 7, 553
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 1 cut(s) 75
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
DraIII CACNNNGTG 1 cut(s) 485
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 114, 277, 289
EarI CTCTTC 3 cut(s) 114, 277, 289
EciI GGCGGA 1 cut(s) 458
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 178
Eco91I GGTNACC 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 138, 531
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 2 cut(s) 10, 556
FaiI YATR 6 cut(s) 8, 101, 233, 365, 528, 554
FatI CATG 2 cut(s) 6, 552
Fnu4HI GCNGC 2 cut(s) 441, 516
FokI GGATG 2 cut(s) 260, 449
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 2 cut(s) 441, 516
FspBI CTAG 2 cut(s) 377, 398
GlaI GCGC 2 cut(s) 32, 80
GluI GCNGC 2 cut(s) 441, 516
HaeII RGCGCY 2 cut(s) 34, 82
HaeIII GGCC 2 cut(s) 5, 308
HapII CCGG 2 cut(s) 209, 279
HgaI GACGC 1 cut(s) 340
HhaI GCGC 2 cut(s) 33, 81
Hin1II CATG 2 cut(s) 10, 556
Hin6I GCGC 2 cut(s) 31, 79
HinP1I GCGC 2 cut(s) 31, 79
HinfI GANTC 4 cut(s) 188, 205, 403, 409
HpaII CCGG 2 cut(s) 209, 279
HphI GGTGA 3 cut(s) 215, 282, 338
Hpy188I TCNGA 6 cut(s) 187, 193, 256, 388, 408, 544
Hpy188III TCNNGA 6 cut(s) 113, 209, 316, 355, 377, 413
Hpy99I CGWCG 3 cut(s) 356, 392, 395
HpyAV CCTTC 5 cut(s) 175, 251, 303, 313, 383
HpyCH4III ACNGT 1 cut(s) 549
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 1 cut(s) 538
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 446
HpyF3I CTNAG 1 cut(s) 75
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 2 cut(s) 10, 556
HspAI GCGC 2 cut(s) 31, 79
Kpn2I TCCGGA 1 cut(s) 208
Kzo9I GATC 1 cut(s) 70
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 1 cut(s) 499
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 3 cut(s) 229, 238, 427
MaeI CTAG 2 cut(s) 377, 398
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 2 cut(s) 221, 326
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 1 cut(s) 180
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 457, 520
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 3 cut(s) 210, 216, 411
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 208
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 209, 279
MspR9I CCNGG 2 cut(s) 140, 533
MvaI CCWGG 2 cut(s) 140, 533
MwoI GCNNNNNNNGC 2 cut(s) 248, 446
NcoI CCATGG 1 cut(s) 6
NdeII GATC 1 cut(s) 70
NlaIII CATG 2 cut(s) 10, 556
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 2 cut(s) 221, 326
PciSI GCTCTTC 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PfeI GAWTC 3 cut(s) 205, 403, 409
PkrI GCNGC 2 cut(s) 442, 517
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 138, 531
PspEI GGTNACC 1 cut(s) 221
PspGI CCWGG 2 cut(s) 138, 531
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 211, 307
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SacI GAGCTC 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 289
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 2 cut(s) 441, 516
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 2 cut(s) 211, 307
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 140, 533
SduI GDGCHC 1 cut(s) 180
SetI ASST 9 cut(s) 167, 180, 198, 219, 295, 375, 399, 504, 517
SfaNI GCATC 3 cut(s) 229, 238, 427
SinI GGWCC 1 cut(s) 211
Sse9I AATT 2 cut(s) 457, 520
SsiI CCGC 3 cut(s) 440, 443, 449
SspMI CTAG 2 cut(s) 377, 398
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 138, 531
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 549
TaiI ACGT 1 cut(s) 219
TaqI TCGA 2 cut(s) 354, 412
TasI AATT 2 cut(s) 457, 520
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 3 cut(s) 205, 403, 409
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TseFI GTSAC 2 cut(s) 221, 326
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 2 cut(s) 221, 326
TspDTI ATGAA 4 cut(s) 132, 138, 525, 541
VpaK11BI GGWCC 1 cut(s) 211
XbaI TCTAGA 1 cut(s) 376
XspI CTAG 2 cut(s) 377, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.